Rh5BG074100

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008848
Physical Location & Seq
Forward (+)
6245801 .. 6249421
3621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG074100.1

Sequence Viewer

Length: 1482 bp
ATGGCAAGAGCAGCTTTGTTCATCATTTTTCTGGTTCTCCAATTACTCCATTTGGGAAGCAGCCGGTCTGTTAAAGACTCTAATCTGCTCATATCCGATGGCTTAGACGACAAGTTGATCAGCCAGTCTTCTTCACTCCTACAAGTAGTGGATGATCTTAAAGCAGATACAGTGACTTGCGACCCCATTTATGGCTTCTTGCCTTGTGCAACTTCAGTCTGGGGGCTGGCTTTCATGGTAATCGTCTATGAGATTTTGCTCTACCTTGCAAATAGCTATATCACAAGTGGATCTGAACTCTTCTTCCACATGTATGGGACCGGATTCTTTGGTGCTAGTGTCTTCCACTTGCTCGGCACAATACCACAAGTGGGAATTGTTGTTGTGACTGGAGTTACAGGAACTGCAGAAACTGCAGAAGCAATGGCTACAATGAATATTGGCATGGTTGCAGGGTCAGCAATCATGCTTCTTACACTAGTGTGGGGTTCTGTTGTTGCATTTGGCAGCTATGACATCTCGGAGTCTTCAACTTCTTCAAATCGAGGACATAAGAAGCGCTTCAGTATCACTGGTTATGGTGTAACAACTGATGCTGAAACCCGCTCTACTGCAAGAATCATGATGGTGTCATTGATCCCCTTTCTCATTCTTCAAGTGGCTAAAGTCCTCCATTCAACCTCAGGGATTCGTATCGTGATCTTAGTTTCTCTACTTGTTTCATTTGCATTCCTTGTCGCTTACTTCATCTATCAGGTCTTTGAGCCATGGATTCAGAACCGGAGACTTGAATTTTTGATGAGTAAATATATACAGAAAACCTTTCTCACTGCAAGCGGCGACCCTAATGTATCAAATATACAAGAGGTGTACGCTAGAATCGACCAGAATCATAATCAGTCTATATCAGCAAAAGAACTGAGAACATTGATCCTAGGAATACAAATAGGGGAAGTAGGTCTGGAGAGTGATTATGTAGAAAAAGCGATGGAGGACTTCGATATATCTGGTGATTCTCAAATATCTGAGCATGAGTTTGTTCATGGAATCTCAAAATGGGTTAATGCAAGGCCCTCTGTCAATCGTCAAGGTCAAGGACATAGAAGGTTTTTAAGCAGCAAGTCCGAGGAAGCTTTGGAAGGGCAGCAGAAGCAGGTGGCTAGAAAAAAGAAGAGTAAGCTTACCAATAAATCTTGGACAAATCAAATCAAGGCTGCTTGTCTCTTGATCCTCGGTATTGGCATTATGATTTTGCTTGCAAATCCCCTCATGGAGACTATCCAAGACTTCTCTACTGCTGCAAGCATCCCTTCATTCTTCACCTCTTATGTTTTGATACCCTTGGCTATCAACTATGGACTGGCATTGCAAATAATAACTTCTGCTCAAGATAAGACTGAGAATGCCATCTCTTTAGCACTTTCAGAGGTCTGTTTCTCTCTTGCACTCATATCTTCTTTCATTTTTGCATCTCTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

493

Amino Acids

53.99

Weight (kDa)

6.16

Isoelectric Point (pI)

33.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 286 - 350 6.8e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1144
Acc36I ACCTGC 1 cut(s) 1144
AccBSI CCGCTC 1 cut(s) 606
AciI CCGC 2 cut(s) 604, 837
AclWI GGATC 4 cut(s) 298, 631, 925, 1222
AcsI RAATTY 1 cut(s) 791
AcuI CTGAAG 2 cut(s) 198, 547
AfaI GTAC 1 cut(s) 872
AfeI AGCGCT 1 cut(s) 560
AfiI CCNNNNNNNGG 2 cut(s) 191, 371
AflIII ACRYGT 1 cut(s) 309
AgsI TTSAA 5 cut(s) 531, 540, 656, 678, 791
AhlI ACTAGT 1 cut(s) 478
AleI CACNNNNGTG 1 cut(s) 481
AloI GAACNNNNNNTCC 2 cut(s) 288, 320
AluBI AGCT 5 cut(s) 14, 276, 510, 1133, 1180
AluI AGCT 5 cut(s) 14, 276, 510, 1133, 1180
Alw26I GTCTC 3 cut(s) 778, 1226, 1268
AlwI GGATC 4 cut(s) 298, 631, 925, 1222
AlwNI CAGNNNCTG 2 cut(s) 404, 413
Aor51HI AGCGCT 1 cut(s) 560
AoxI GGCC 1 cut(s) 1070
ApeKI GCWGC 7 cut(s) 11, 60, 507, 1116, 1144, 1214, 1298
ApoI RAATTY 1 cut(s) 791
Asp700I GAANNNNTTC 1 cut(s) 560
AspA2I CCTAGG 1 cut(s) 934
AspLEI GCGC 1 cut(s) 561
AspS9I GGNCC 2 cut(s) 318, 1071
AsuHPI GGTGA 2 cut(s) 1022, 1312
AvaII GGWCC 1 cut(s) 318
AvrII CCTAGG 1 cut(s) 934
AxyI CCTNAGG 1 cut(s) 682
BbsI GAAGAC 3 cut(s) 120, 334, 519
BbvI GCAGC 7 cut(s) 23, 72, 519, 1128, 1156, 1201, 1285
BccI CCATC 4 cut(s) 92, 619, 982, 1415
BclI TGATCA 1 cut(s) 117
BcoDI GTCTC 3 cut(s) 778, 1226, 1268
BcuI ACTAGT 1 cut(s) 478
BfaI CTAG 5 cut(s) 336, 479, 876, 935, 1161
BfmI CTRYAG 2 cut(s) 405, 414
BfoI RGCGCY 1 cut(s) 562
BfuAI ACCTGC 1 cut(s) 1144
BisI GCNGC 8 cut(s) 12, 61, 508, 838, 1117, 1145, 1215, 1299
BlnI CCTAGG 1 cut(s) 934
BlsI GCNGC 8 cut(s) 13, 62, 509, 839, 1118, 1146, 1216, 1300
Bme18I GGWCC 1 cut(s) 318
BmgT120I GGNCC 2 cut(s) 318, 1071
BmiI GGNNCC 1 cut(s) 319
BmsI GCATC 3 cut(s) 583, 1314, 1478
BpiI GAAGAC 3 cut(s) 120, 334, 519
BpmI CTGGAG 2 cut(s) 411, 983
BpuEI CTTGAG 1 cut(s) 1371
BsaBI GATNNNNATC 1 cut(s) 692
BsaJI CCNNGG 5 cut(s) 767, 934, 1125, 1231, 1341
BsaWI WCCGGW 2 cut(s) 320, 780
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 371
Bse118I RCCGGY 1 cut(s) 63
Bse1I ACTGG 4 cut(s) 124, 394, 577, 1365
Bse21I CCTNAGG 1 cut(s) 682
Bse3DI GCAATG 2 cut(s) 429, 1364
Bse8I GATNNNNATC 1 cut(s) 692
BseDI CCNNGG 5 cut(s) 767, 934, 1125, 1231, 1341
BseGI GGATG 2 cut(s) 157, 1305
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 2 cut(s) 191, 371
BseMI GCAATG 2 cut(s) 429, 1364
BseMII CTCAG 4 cut(s) 696, 911, 1017, 1389
BseNI ACTGG 4 cut(s) 124, 394, 577, 1365
BseXI GCAGC 7 cut(s) 23, 72, 519, 1128, 1156, 1201, 1285
BshFI GGCC 1 cut(s) 1072
BsiSI CCGG 3 cut(s) 64, 321, 781
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 2 cut(s) 191, 371
BsmAI GTCTC 3 cut(s) 778, 1226, 1268
BsmFI GGGAC 1 cut(s) 331
BsmI GAATGC 2 cut(s) 728, 1408
BsnI GGCC 1 cut(s) 1072
Bsp143I GATC 7 cut(s) 117, 154, 290, 636, 699, 930, 1227
Bsp19I CCATGG 1 cut(s) 767
BspACI CCGC 2 cut(s) 604, 837
BspANI GGCC 1 cut(s) 1072
BspCNI CTCAG 4 cut(s) 695, 912, 1018, 1390
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 1 cut(s) 319
BspMAI CTGCAG 2 cut(s) 409, 418
BspMI ACCTGC 1 cut(s) 1144
BspPI GGATC 4 cut(s) 298, 631, 925, 1222
BsrBI CCGCTC 1 cut(s) 606
BsrDI GCAATG 2 cut(s) 429, 1364
BsrFI RCCGGY 1 cut(s) 63
BsrI ACTGG 4 cut(s) 124, 394, 577, 1365
BssAI RCCGGY 1 cut(s) 63
BssECI CCNNGG 5 cut(s) 767, 934, 1125, 1231, 1341
BssMI GATC 7 cut(s) 117, 154, 290, 636, 699, 930, 1227
BssT1I CCWWGG 3 cut(s) 767, 934, 1341
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 2 cut(s) 305, 1166
BstAPI GCANNNNNTGC 1 cut(s) 413
BstC8I GCNNGC 4 cut(s) 228, 835, 1257, 1303
BstDEI CTNAG 6 cut(s) 103, 682, 703, 920, 1026, 1398
BstDSI CCRYGG 1 cut(s) 767
BstF5I GGATG 2 cut(s) 157, 1305
BstH2I RGCGCY 1 cut(s) 562
BstHHI GCGC 1 cut(s) 561
BstKTI GATC 7 cut(s) 120, 157, 293, 639, 702, 933, 1230
BstMAI GTCTC 3 cut(s) 778, 1226, 1268
BstMBI GATC 7 cut(s) 117, 154, 290, 636, 699, 930, 1227
BstMWI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1150
BstNSI RCATGY 1 cut(s) 313
BstSFI CTRYAG 2 cut(s) 405, 414
BstV1I GCAGC 7 cut(s) 23, 72, 519, 1128, 1156, 1201, 1285
BstV2I GAAGAC 3 cut(s) 120, 334, 519
BstX2I RGATCY 1 cut(s) 290
BstXI CCANNNNNNTGG 1 cut(s) 314
BstYI RGATCY 1 cut(s) 290
Bsu36I CCTNAGG 1 cut(s) 682
BsuRI GGCC 1 cut(s) 1072
BtgI CCRYGG 1 cut(s) 767
BtgZI GCGATG 1 cut(s) 1001
BtsCI GGATG 2 cut(s) 157, 1305
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 3 cut(s) 177, 570, 828
BveI ACCTGC 1 cut(s) 1144
Cac8I GCNNGC 4 cut(s) 228, 835, 1257, 1303
CaiI CAGNNNCTG 2 cut(s) 404, 413
CciI TCATGA 1 cut(s) 621
CfoI GCGC 1 cut(s) 561
Cfr10I RCCGGY 1 cut(s) 63
Cfr13I GGNCC 2 cut(s) 318, 1071
Csp6I GTAC 1 cut(s) 871
CviAII CATG 9 cut(s) 235, 310, 445, 466, 622, 768, 1031, 1043, 1270
CviQI GTAC 1 cut(s) 871
DdeI CTNAG 6 cut(s) 103, 682, 703, 920, 1026, 1398
DpnI GATC 7 cut(s) 119, 156, 292, 638, 701, 932, 1229
DpnII GATC 7 cut(s) 117, 154, 290, 636, 699, 930, 1227
Eam1104I CTCTTC 2 cut(s) 305, 1166
EarI CTCTTC 2 cut(s) 305, 1166
Eco130I CCWWGG 3 cut(s) 767, 934, 1341
Eco47I GGWCC 1 cut(s) 318
Eco47III AGCGCT 1 cut(s) 560
Eco57I CTGAAG 2 cut(s) 198, 547
Eco81I CCTNAGG 1 cut(s) 682
EcoO109I RGGNCCY 1 cut(s) 1071
EcoT14I CCWWGG 3 cut(s) 767, 934, 1341
ErhI CCWWGG 3 cut(s) 767, 934, 1341
FaeI CATG 9 cut(s) 238, 313, 448, 469, 625, 771, 1034, 1046, 1273
FalI AAGNNNNNCTT 5 cut(s) 30, 545, 577, 1294, 1326
FaqI GGGAC 1 cut(s) 331
FatI CATG 9 cut(s) 234, 309, 444, 465, 621, 767, 1030, 1042, 1269
FauI CCCGC 1 cut(s) 611
FbaI TGATCA 1 cut(s) 117
Fnu4HI GCNGC 8 cut(s) 12, 61, 508, 838, 1117, 1145, 1215, 1299
FokI GGATG 2 cut(s) 164, 1292
Fsp4HI GCNGC 8 cut(s) 12, 61, 508, 838, 1117, 1145, 1215, 1299
FspBI CTAG 5 cut(s) 336, 479, 876, 935, 1161
GlaI GCGC 1 cut(s) 560
GluI GCNGC 8 cut(s) 12, 61, 508, 838, 1117, 1145, 1215, 1299
GsuI CTGGAG 2 cut(s) 411, 983
HaeII RGCGCY 1 cut(s) 562
HaeIII GGCC 1 cut(s) 1072
HapII CCGG 3 cut(s) 64, 321, 781
HhaI GCGC 1 cut(s) 561
Hin1II CATG 9 cut(s) 238, 313, 448, 469, 625, 771, 1034, 1046, 1273
Hin6I GCGC 1 cut(s) 559
HinP1I GCGC 1 cut(s) 559
HindIII AAGCTT 2 cut(s) 1131, 1178
HpaII CCGG 3 cut(s) 64, 321, 781
HphI GGTGA 2 cut(s) 1022, 1312
Hpy166II GTNNAC 1 cut(s) 871
Hpy188I TCNGA 8 cut(s) 97, 295, 523, 777, 1027, 1126, 1426, 1476
Hpy188III TCNNGA 5 cut(s) 622, 697, 962, 1225, 1388
Hpy8I GTNNAC 1 cut(s) 871
HpyAV CCTTC 3 cut(s) 1098, 1133, 1320
HpyCH4III ACNGT 1 cut(s) 172
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1150
HpyF3I CTNAG 6 cut(s) 103, 682, 703, 920, 1026, 1398
Hsp92II CATG 9 cut(s) 238, 313, 448, 469, 625, 771, 1034, 1046, 1273
HspAI GCGC 1 cut(s) 559
Ksp22I TGATCA 1 cut(s) 117
Kzo9I GATC 7 cut(s) 117, 154, 290, 636, 699, 930, 1227
Lsp1109I GCAGC 7 cut(s) 23, 72, 519, 1128, 1156, 1201, 1285
LweI GCATC 3 cut(s) 583, 1314, 1478
MaeI CTAG 5 cut(s) 336, 479, 876, 935, 1161
MaeIII GTNAC 4 cut(s) 172, 385, 394, 583
MalI GATC 7 cut(s) 119, 156, 292, 638, 701, 932, 1229
MbiI CCGCTC 1 cut(s) 606
MboI GATC 7 cut(s) 117, 154, 290, 636, 699, 930, 1227
MflI RGATCY 1 cut(s) 290
MluCI AATT 3 cut(s) 41, 375, 791
MlyI GAGTC 2 cut(s) 71, 533
MroXI GAANNNNTTC 1 cut(s) 560
MseI TTAA 4 cut(s) 72, 159, 1062, 1112
MslI CAYNNNNRTG 3 cut(s) 312, 481, 626
MspI CCGG 3 cut(s) 64, 321, 781
Mva1269I GAATGC 2 cut(s) 728, 1408
MwoI GCNNNNNNNGC 4 cut(s) 11, 413, 458, 1150
NcoI CCATGG 1 cut(s) 767
NdeII GATC 7 cut(s) 117, 154, 290, 636, 699, 930, 1227
NlaIII CATG 9 cut(s) 238, 313, 448, 469, 625, 771, 1034, 1046, 1273
NlaIV GGNNCC 1 cut(s) 319
NmeAIII GCCGAG 1 cut(s) 333
NmuCI GTSAC 2 cut(s) 172, 385
NspI RCATGY 1 cut(s) 313
OliI CACNNNNGTG 1 cut(s) 481
PagI TCATGA 1 cut(s) 621
PaqCI CACCTGC 1 cut(s) 1144
PciI ACATGT 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 879
PctI GAATGC 2 cut(s) 728, 1408
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 8 cut(s) 324, 618, 688, 772, 879, 889, 1013, 1047
PkrI GCNGC 8 cut(s) 13, 62, 509, 839, 1118, 1146, 1216, 1300
PleI GAGTC 2 cut(s) 71, 532
PpsI GAGTC 2 cut(s) 71, 532
PscI ACATGT 1 cut(s) 309
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 2 cut(s) 318, 1071
PstI CTGCAG 2 cut(s) 409, 418
PstNI CAGNNNCTG 2 cut(s) 404, 413
PsuI RGATCY 1 cut(s) 290
RsaI GTAC 1 cut(s) 872
RsaNI GTAC 1 cut(s) 871
RseI CAYNNNNRTG 3 cut(s) 312, 481, 626
SaqAI TTAA 4 cut(s) 72, 159, 1062, 1112
SatI GCNGC 8 cut(s) 12, 61, 508, 838, 1117, 1145, 1215, 1299
Sau3AI GATC 7 cut(s) 117, 154, 290, 636, 699, 930, 1227
Sau96I GGNCC 2 cut(s) 318, 1071
SchI GAGTC 2 cut(s) 71, 533
SfaNI GCATC 3 cut(s) 583, 1314, 1478
SfcI CTRYAG 2 cut(s) 405, 414
SinI GGWCC 1 cut(s) 318
SmiMI CAYNNNNRTG 3 cut(s) 312, 481, 626
SmlI CTYRAG 1 cut(s) 1386
SmoI CTYRAG 1 cut(s) 1386
SpeI ACTAGT 1 cut(s) 478
Sse9I AATT 3 cut(s) 41, 375, 791
SsiI CCGC 2 cut(s) 604, 837
SspI AATATT 1 cut(s) 439
SspMI CTAG 5 cut(s) 336, 479, 876, 935, 1161
StyI CCWWGG 3 cut(s) 767, 934, 1341
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 3 cut(s) 544, 882, 999
TasI AATT 3 cut(s) 41, 375, 791
TauI GCSGC 1 cut(s) 840
TfiI GAWTC 8 cut(s) 324, 618, 688, 772, 879, 889, 1013, 1047
Tru1I TTAA 4 cut(s) 72, 159, 1062, 1112
Tru9I TTAA 4 cut(s) 72, 159, 1062, 1112
TscAI CASTG 3 cut(s) 177, 577, 835
TseFI GTSAC 2 cut(s) 172, 385
TseI GCWGC 7 cut(s) 11, 60, 507, 1116, 1144, 1214, 1298
Tsp45I GTSAC 2 cut(s) 172, 385
TspDTI ATGAA 8 cut(s) 10, 223, 449, 711, 736, 1031, 1302, 1450
TspRI CASTG 3 cut(s) 177, 577, 835
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 1 cut(s) 791
XceI RCATGY 1 cut(s) 313
XmaJI CCTAGG 1 cut(s) 934
XmnI GAANNNNTTC 1 cut(s) 560
XspI CTAG 5 cut(s) 336, 479, 876, 935, 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.