pycom05g28280

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008848
Physical Location & Seq
Reverse (-)
29052996 .. 29054778
1783 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g28280.1

Sequence Viewer

Length: 846 bp
ATGCTTGGCTTGATTCCACAAGTTGGAATGGTTCTTGTTACCGGAGTTACAGCAACTTCAACAACTGTGGAGGAAATGGCAGAAATGAGCATGGGCATGCTTGCAGGATCAACAATCATGATTCTTACCCTAATTTGGGGTTCTGTTGTTGCTTTCGGCAGCTACGACCTCTCGGATTCTCAAACTTCATCAAATCCAGAAAATAAGAAACCCTTCAGTTTAACTGGTTCTGGTGTAAGAACTGATGTCGAAACCCAAGATACTGCAAGAATCATGATGCTGTCGTTGATCCCATTTCTCATTCTTCAACTTGCAAAAATCTTGACTTCAACCAGAGCAGTTCGAGTTGTGATCTTAGTTTCTTTACTGATTGCATCTGCATTCCTTGTCAATTACTTCATCTACCAGTTCTTTCAGCCATGGATTCAGAGCAGAAGGCATGAATATTTGATGCGTAAATACAGACCGGAAAACATACTGCCAAGTCTTCTGACAGTACGCGGAAACCCTAATGTATCCAACATAAAAGGGCTGTTCCATAGAATAGACAAGAATCATAGCACATACATATCAGCAGATGAACTTAGAGCATCGATCCTAGGAATACAAATAGAAGAATTTGGTCTGGATGACGATGATTATGTAGCAGAAGTGATGAAGCAATTTGATATCTCTGGCGACGCTCAAATAGCTGAGACGGACTTTGTGAATGGAATCTCAAAATGGATTAATCCAGTTATGCCCTCTGCCAACAGTAAAGGTCATGAACATAAATCGCTTTTCAGAAGAAATAATTCAAAGAATGAGAAAACTAAGGAAGATCAGCAGACGCCGCTCGATCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

282

Amino Acids

31.43

Weight (kDa)

5.77

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 174 - 239 1.9e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 23
AccBSI CCGCTC 1 cut(s) 835
AccII CGCG 1 cut(s) 501
AciI CCGC 2 cut(s) 501, 833
AclWI GGATC 3 cut(s) 115, 283, 589
AcsI RAATTY 1 cut(s) 617
AcuI CTGAAG 1 cut(s) 199
AcyI GRCGYC 1 cut(s) 830
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 3 cut(s) 23, 135, 136
AgsI TTSAA 4 cut(s) 60, 308, 330, 798
AluBI AGCT 2 cut(s) 162, 692
AluI AGCT 2 cut(s) 162, 692
Alw26I GTCTC 1 cut(s) 689
AlwI GGATC 3 cut(s) 115, 283, 589
ApeKI GCWGC 1 cut(s) 159
ApoI RAATTY 1 cut(s) 617
AseI ATTAAT 1 cut(s) 729
Asp700I GAANNNNTTC 2 cut(s) 212, 793
AspA2I CCTAGG 1 cut(s) 598
AvrII CCTAGG 1 cut(s) 598
BbsI GAAGAC 1 cut(s) 479
BbvI GCAGC 1 cut(s) 171
BciVI GTATCC 1 cut(s) 526
BcoDI GTCTC 1 cut(s) 689
BfaI CTAG 1 cut(s) 599
BfuI GTATCC 1 cut(s) 526
BisI GCNGC 2 cut(s) 160, 833
BlnI CCTAGG 1 cut(s) 598
BlsI GCNGC 2 cut(s) 161, 834
BmsI GCATC 4 cut(s) 267, 383, 441, 599
BpiI GAAGAC 1 cut(s) 479
Bsa29I ATCGAT 1 cut(s) 593
BsaHI GRCGYC 1 cut(s) 830
BsaJI CCNNGG 2 cut(s) 419, 598
BsaWI WCCGGW 2 cut(s) 41, 466
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 135, 136
Bse1I ACTGG 3 cut(s) 229, 406, 734
BseCI ATCGAT 1 cut(s) 593
BseDI CCNNGG 2 cut(s) 419, 598
BseGI GGATG 1 cut(s) 634
BseLI CCNNNNNNNGG 3 cut(s) 23, 135, 136
BseMII CTCAG 1 cut(s) 684
BseNI ACTGG 3 cut(s) 229, 406, 734
BseXI GCAGC 1 cut(s) 171
Bsh1236I CGCG 1 cut(s) 501
BshVI ATCGAT 1 cut(s) 593
BsiSI CCGG 2 cut(s) 42, 467
BslI CCNNNNNNNGG 3 cut(s) 23, 135, 136
BsmAI GTCTC 1 cut(s) 689
BsmBI CGTCTC 1 cut(s) 689
BsmI GAATGC 1 cut(s) 380
Bsp143I GATC 6 cut(s) 107, 288, 351, 594, 820, 838
Bsp19I CCATGG 1 cut(s) 419
BspACI CCGC 2 cut(s) 501, 833
BspCNI CTCAG 1 cut(s) 685
BspDI ATCGAT 1 cut(s) 593
BspFNI CGCG 1 cut(s) 501
BspHI TCATGA 3 cut(s) 117, 273, 763
BspPI GGATC 3 cut(s) 115, 283, 589
BsrBI CCGCTC 1 cut(s) 835
BsrI ACTGG 3 cut(s) 229, 406, 734
BssECI CCNNGG 2 cut(s) 419, 598
BssMI GATC 6 cut(s) 107, 288, 351, 594, 820, 838
BssNI GRCGYC 1 cut(s) 830
BssT1I CCWWGG 2 cut(s) 419, 598
Bst4CI ACNGT 3 cut(s) 67, 496, 755
BstACI GRCGYC 1 cut(s) 830
BstC8I GCNNGC 2 cut(s) 98, 102
BstDEI CTNAG 4 cut(s) 355, 584, 693, 813
BstDSI CCRYGG 1 cut(s) 419
BstF5I GGATG 1 cut(s) 634
BstFNI CGCG 1 cut(s) 501
BstKTI GATC 6 cut(s) 110, 291, 354, 597, 823, 841
BstMAI GTCTC 1 cut(s) 689
BstMBI GATC 6 cut(s) 107, 288, 351, 594, 820, 838
BstMWI GCNNNNNNNGC 2 cut(s) 689, 832
BstNSI RCATGY 1 cut(s) 100
BstUI CGCG 1 cut(s) 501
BstV1I GCAGC 1 cut(s) 171
BstV2I GAAGAC 1 cut(s) 479
Bsu15I ATCGAT 1 cut(s) 593
BsuI GTATCC 1 cut(s) 526
BsuTUI ATCGAT 1 cut(s) 593
BtgI CCRYGG 1 cut(s) 419
BtsCI GGATG 1 cut(s) 634
Cac8I GCNNGC 2 cut(s) 98, 102
CciI TCATGA 3 cut(s) 117, 273, 763
ClaI ATCGAT 1 cut(s) 593
CseI GACGC 2 cut(s) 689, 838
Csp6I GTAC 1 cut(s) 497
CspCI CAANNNNNGTGG 2 cut(s) 48, 83
CviAII CATG 7 cut(s) 91, 97, 118, 274, 420, 440, 764
CviJI RGCY 5 cut(s) 9, 162, 418, 532, 692
CviKI_1 RGCY 5 cut(s) 9, 162, 418, 532, 692
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 4 cut(s) 355, 584, 693, 813
DpnI GATC 6 cut(s) 109, 290, 353, 596, 822, 840
DpnII GATC 6 cut(s) 107, 288, 351, 594, 820, 838
Eco130I CCWWGG 2 cut(s) 419, 598
Eco32I GATATC 1 cut(s) 670
Eco57I CTGAAG 1 cut(s) 199
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 2 cut(s) 419, 598
ErhI CCWWGG 2 cut(s) 419, 598
Esp3I CGTCTC 1 cut(s) 689
FaeI CATG 7 cut(s) 94, 100, 121, 277, 423, 443, 767
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FatI CATG 7 cut(s) 90, 96, 117, 273, 419, 439, 763
Fnu4HI GCNGC 2 cut(s) 160, 833
FokI GGATG 1 cut(s) 641
Fsp4HI GCNGC 2 cut(s) 160, 833
FspBI CTAG 1 cut(s) 599
GluI GCNGC 2 cut(s) 160, 833
HapII CCGG 2 cut(s) 42, 467
HgaI GACGC 2 cut(s) 689, 838
Hin1I GRCGYC 1 cut(s) 830
Hin1II CATG 7 cut(s) 94, 100, 121, 277, 423, 443, 767
HinfI GANTC 7 cut(s) 13, 121, 176, 270, 424, 553, 714
HpaII CCGG 2 cut(s) 42, 467
Hpy188I TCNGA 4 cut(s) 175, 429, 492, 785
Hpy188III TCNNGA 6 cut(s) 118, 197, 274, 322, 626, 764
Hpy99I CGWCG 1 cut(s) 683
HpyAV CCTTC 2 cut(s) 223, 429
HpyCH4III ACNGT 3 cut(s) 67, 496, 755
HpyCH4V TGCA 5 cut(s) 104, 266, 314, 374, 380
HpyF10VI GCNNNNNNNGC 2 cut(s) 689, 832
HpyF3I CTNAG 4 cut(s) 355, 584, 693, 813
Hsp92I GRCGYC 1 cut(s) 830
Hsp92II CATG 7 cut(s) 94, 100, 121, 277, 423, 443, 767
Kzo9I GATC 6 cut(s) 107, 288, 351, 594, 820, 838
Lsp1109I GCAGC 1 cut(s) 171
LweI GCATC 4 cut(s) 267, 383, 441, 599
MaeI CTAG 1 cut(s) 599
MaeIII GTNAC 2 cut(s) 37, 46
MalI GATC 6 cut(s) 109, 290, 353, 596, 822, 840
MbiI CCGCTC 1 cut(s) 835
MboI GATC 6 cut(s) 107, 288, 351, 594, 820, 838
MboII GAAGA 5 cut(s) 296, 479, 626, 798, 830
MluCI AATT 5 cut(s) 132, 391, 617, 662, 793
MmeI TCCRAC 2 cut(s) 4, 543
MnlI CCTC 3 cut(s) 64, 179, 754
MroXI GAANNNNTTC 2 cut(s) 212, 793
MseI TTAA 2 cut(s) 221, 729
MslI CAYNNNNRTG 1 cut(s) 95
MspI CCGG 2 cut(s) 42, 467
Mva1269I GAATGC 1 cut(s) 380
MvnI CGCG 1 cut(s) 501
MwoI GCNNNNNNNGC 2 cut(s) 689, 832
NcoI CCATGG 1 cut(s) 419
NdeII GATC 6 cut(s) 107, 288, 351, 594, 820, 838
NlaIII CATG 7 cut(s) 94, 100, 121, 277, 423, 443, 767
NspI RCATGY 1 cut(s) 100
PaeI GCATGC 1 cut(s) 100
PagI TCATGA 3 cut(s) 117, 273, 763
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 1 cut(s) 380
PdmI GAANNNNTTC 2 cut(s) 212, 793
PfeI GAWTC 7 cut(s) 13, 121, 176, 270, 424, 553, 714
PflMI CCANNNNNTGG 1 cut(s) 23
PkrI GCNGC 2 cut(s) 161, 834
PshBI ATTAAT 1 cut(s) 729
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
RseI CAYNNNNRTG 1 cut(s) 95
SaqAI TTAA 2 cut(s) 221, 729
SatI GCNGC 2 cut(s) 160, 833
Sau3AI GATC 6 cut(s) 107, 288, 351, 594, 820, 838
SetI ASST 4 cut(s) 164, 171, 694, 763
SfaNI GCATC 4 cut(s) 267, 383, 441, 599
SmiMI CAYNNNNRTG 1 cut(s) 95
SphI GCATGC 1 cut(s) 100
Sse9I AATT 5 cut(s) 132, 391, 617, 662, 793
SsiI CCGC 2 cut(s) 501, 833
SspI AATATT 1 cut(s) 446
SspMI CTAG 1 cut(s) 599
StyI CCWWGG 2 cut(s) 419, 598
TaaI ACNGT 3 cut(s) 67, 496, 755
TaqI TCGA 4 cut(s) 249, 343, 593, 837
TasI AATT 5 cut(s) 132, 391, 617, 662, 793
TauI GCSGC 1 cut(s) 835
TfiI GAWTC 7 cut(s) 13, 121, 176, 270, 424, 553, 714
Tru1I TTAA 2 cut(s) 221, 729
Tru9I TTAA 2 cut(s) 221, 729
TseI GCWGC 1 cut(s) 159
TspDTI ATGAA 6 cut(s) 177, 388, 456, 594, 671, 780
TspGWI ACGGA 1 cut(s) 713
Van91I CCANNNNNTGG 1 cut(s) 23
VspI ATTAAT 1 cut(s) 729
XapI RAATTY 1 cut(s) 617
XceI RCATGY 1 cut(s) 100
XmaJI CCTAGG 1 cut(s) 598
XmnI GAANNNNTTC 2 cut(s) 212, 793
XspI CTAG 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.