Rh5AG079300

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0008848
Physical Location & Seq
Forward (+)
6799890 .. 6803607
3718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG079300.1

Sequence Viewer

Length: 1332 bp
ATGTGCAGAAATCATGTATCTTTGGTCTCAATGACTGGAGTTACAGGAACTGCAGAAACTGCAGAAGCAATGGCTACAATGAACATTGGCATGGTTGCAGGGTCAGCAATCATGCTTCTTACATTAGTCTGGGGTTCTGTTGTTGCATTTGGCAGCTATGACATCTCCAAGTCTCCAACTTCTTCAAATCGAGGACATAAGAAGCGCTTCAGTATCACTGGTTATGTAACAACTGATGCTGAAACCAGCTGTACCGCAAGAATCATGATGGTGTCATTGATCCCCTTTCTTATTCTTCAAGTGGCTAAAGTCCTCAATTCAACCTCAGGGATTCGTATTGTGATCTTAATTTCTCTACTAGTTTCATTTGCATTCCTTGTCGCTTACTGCATCTATCAGGTCTTTGAGCCATGGATTCAGAACAGGAGACTTGAATTTTTGATGAGTAAATATGTACAGAGAACCTTGTCACCGAGTTTTCTCACTGCAAGCGGCGAGCCTAATGTATCAAATATACTAGAGGTGTTTTCTAGAATCGACCAGAATCATAATCAGTCTATATCAGCAAAAGAACTGAGAACATTGATCCTAGGAATACAAATAGGGGAAGTAGGTCTGGAGAGTGATTATGTAGAAAAGGTGATGGAGGACTTCGATATATCTGGTGATTCTCAAATATCTGAGCAAGAGTTTGTTAATGGAATCTCAAAATGGGTTAATGCAAGGCCCTCTGTCAATCGTCAAGGTCAAGGAGATAGAAGGTTTTTAAGCAGCAAGTCCGAGGAAGCTTTGGAAGGGCAGCAGAAGCAGGTGGCTAGAAAAAAGAAGAGTAAGCTTACCGATAAATCTTGGACAAATCAAATCAAGGCTGCTTGTCTCTTGATCCTCGGTATTGGCATTATGATTTTGCTTGCAAATCCCCTCATGGAGACTATCCAAGACTTCTCTACTGCTGCAAGCATCCCTTCATTCTTCACCTCTTATGTTTTGATACCCTTGGCTATCAACTATGGACTGGCATTGCAAATAATAACTTCTGCTCAAGATAAGACTGAGAATGCCATCTCTTTAGCACTTTCAGAGATTTATAACAGCGTGTTCATGAACAACGTTATGGGATTGACCATCTTCTTGGCACTTGTTTACATACGTAATTTATCTTGGGATGTCTCTGCATCAGTTTTAGTTGTTCTGATCATTTGCTCAGTAATGGGTCTTTATGCCAGCTTCAGCAGAAAGTTCCCATTTTGGACCTGTATTATAGCATACATTTTGTACCCCATATCACTGTTGATGGTTTATATTCTGACCACTTTTCTTGGATGGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_7 Na_Ca_ex

Protein Analysis

443

Amino Acids

49.05

Weight (kDa)

8.41

Isoelectric Point (pI)

36.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 171 - 235 3.3e-11 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1089
AarI CACCTGC 1 cut(s) 799
Acc36I ACCTGC 1 cut(s) 799
AciI CCGC 2 cut(s) 255, 492
AclI AACGTT 1 cut(s) 1110
AclWI GGATC 3 cut(s) 274, 580, 877
AcsI RAATTY 1 cut(s) 434
AcuI CTGAAG 2 cut(s) 193, 1213
AfaI GTAC 3 cut(s) 253, 456, 1277
AfeI AGCGCT 1 cut(s) 206
AgsI TTSAA 4 cut(s) 186, 299, 321, 434
AhlI ACTAGT 1 cut(s) 358
AluBI AGCT 5 cut(s) 156, 249, 788, 835, 1227
AluI AGCT 5 cut(s) 156, 249, 788, 835, 1227
Alw26I GTCTC 6 cut(s) 31, 177, 421, 881, 923, 1174
AlwI GGATC 3 cut(s) 274, 580, 877
AlwNI CAGNNNCTG 2 cut(s) 50, 59
Aor51HI AGCGCT 1 cut(s) 206
AoxI GGCC 1 cut(s) 725
ApeKI GCWGC 5 cut(s) 153, 771, 799, 869, 953
ApoI RAATTY 1 cut(s) 434
Asp700I GAANNNNTTC 1 cut(s) 206
AspA2I CCTAGG 1 cut(s) 589
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 726, 1251
AsuHPI GGTGA 4 cut(s) 462, 652, 677, 967
AvaII GGWCC 1 cut(s) 1251
AvrII CCTAGG 1 cut(s) 589
AxyI CCTNAGG 1 cut(s) 325
BbvI GCAGC 5 cut(s) 165, 783, 811, 856, 940
BccI CCATC 6 cut(s) 262, 637, 1070, 1133, 1288, 1317
BclI TGATCA 1 cut(s) 1194
BcoDI GTCTC 6 cut(s) 31, 177, 421, 881, 923, 1174
BcuI ACTAGT 1 cut(s) 358
BfaI CTAG 5 cut(s) 359, 518, 531, 590, 816
BfmI CTRYAG 2 cut(s) 51, 60
BfoI RGCGCY 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 799
BisI GCNGC 6 cut(s) 154, 493, 772, 800, 870, 954
BlnI CCTAGG 1 cut(s) 589
BlsI GCNGC 6 cut(s) 155, 494, 773, 801, 871, 955
Bme18I GGWCC 1 cut(s) 1251
BmgT120I GGNCC 2 cut(s) 726, 1251
BmsI GCATC 4 cut(s) 226, 399, 969, 1184
BpmI CTGGAG 2 cut(s) 57, 638
BpuEI CTTGAG 1 cut(s) 1026
BsaAI YACGTR 1 cut(s) 1151
BsaI GGTCTC 1 cut(s) 31
BsaJI CCNNGG 5 cut(s) 410, 589, 780, 886, 996
Bse1I ACTGG 3 cut(s) 40, 223, 1020
Bse21I CCTNAGG 1 cut(s) 325
Bse3DI GCAATG 2 cut(s) 75, 1019
BseDI CCNNGG 5 cut(s) 410, 589, 780, 886, 996
BseGI GGATG 3 cut(s) 960, 1171, 1328
BseMI GCAATG 2 cut(s) 75, 1019
BseMII CTCAG 5 cut(s) 339, 566, 672, 1044, 1218
BseNI ACTGG 3 cut(s) 40, 223, 1020
BseXI GCAGC 5 cut(s) 165, 783, 811, 856, 940
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 1 cut(s) 727
BsmAI GTCTC 6 cut(s) 31, 177, 421, 881, 923, 1174
BsmI GAATGC 2 cut(s) 371, 1063
BsnI GGCC 1 cut(s) 727
Bso31I GGTCTC 1 cut(s) 31
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 5 cut(s) 279, 342, 585, 882, 1194
Bsp19I CCATGG 1 cut(s) 410
BspACI CCGC 2 cut(s) 255, 492
BspANI GGCC 1 cut(s) 727
BspCNI CTCAG 5 cut(s) 338, 567, 673, 1045, 1217
BspHI TCATGA 2 cut(s) 264, 1101
BspMAI CTGCAG 2 cut(s) 55, 64
BspMI ACCTGC 1 cut(s) 799
BspPI GGATC 3 cut(s) 274, 580, 877
BspTNI GGTCTC 1 cut(s) 31
BsrDI GCAATG 2 cut(s) 75, 1019
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 3 cut(s) 40, 223, 1020
BssECI CCNNGG 5 cut(s) 410, 589, 780, 886, 996
BssMI GATC 5 cut(s) 279, 342, 585, 882, 1194
BssT1I CCWWGG 3 cut(s) 410, 589, 996
Bst4CI ACNGT 1 cut(s) 1290
Bst6I CTCTTC 1 cut(s) 821
BstAPI GCANNNNNTGC 1 cut(s) 59
BstAUI TGTACA 1 cut(s) 454
BstBAI YACGTR 1 cut(s) 1151
BstC8I GCNNGC 5 cut(s) 490, 497, 912, 958, 1225
BstDEI CTNAG 6 cut(s) 325, 575, 681, 1053, 1204, 1329
BstDSI CCRYGG 1 cut(s) 410
BstF5I GGATG 3 cut(s) 960, 1171, 1328
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 5 cut(s) 282, 345, 588, 885, 1197
BstMAI GTCTC 6 cut(s) 31, 177, 421, 881, 923, 1174
BstMBI GATC 5 cut(s) 279, 342, 585, 882, 1194
BstMWI GCNNNNNNNGC 3 cut(s) 59, 104, 805
BstSFI CTRYAG 2 cut(s) 51, 60
BstSNI TACGTA 1 cut(s) 1151
BstV1I GCAGC 5 cut(s) 165, 783, 811, 856, 940
BstXI CCANNNNNNTGG 1 cut(s) 1132
Bsu36I CCTNAGG 1 cut(s) 325
BsuRI GGCC 1 cut(s) 727
BtgI CCRYGG 1 cut(s) 410
BtsCI GGATG 3 cut(s) 960, 1171, 1328
BtsI GCAGTG 1 cut(s) 483
BtsIMutI CAGTG 3 cut(s) 216, 483, 1286
BveI ACCTGC 1 cut(s) 799
Cac8I GCNNGC 5 cut(s) 490, 497, 912, 958, 1225
CaiI CAGNNNCTG 2 cut(s) 50, 59
CciI TCATGA 2 cut(s) 264, 1101
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 2 cut(s) 726, 1251
Csp6I GTAC 3 cut(s) 252, 455, 1276
CspCI CAANNNNNGTGG 1 cut(s) 1300
CviAII CATG 7 cut(s) 14, 91, 112, 265, 411, 925, 1102
CviQI GTAC 3 cut(s) 252, 455, 1276
DdeI CTNAG 6 cut(s) 325, 575, 681, 1053, 1204, 1329
DpnI GATC 5 cut(s) 281, 344, 587, 884, 1196
DpnII GATC 5 cut(s) 279, 342, 585, 882, 1194
Eam1104I CTCTTC 1 cut(s) 821
EarI CTCTTC 1 cut(s) 821
Eco105I TACGTA 1 cut(s) 1151
Eco130I CCWWGG 3 cut(s) 410, 589, 996
Eco31I GGTCTC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 1251
Eco47III AGCGCT 1 cut(s) 206
Eco57I CTGAAG 2 cut(s) 193, 1213
Eco81I CCTNAGG 1 cut(s) 325
EcoO109I RGGNCCY 1 cut(s) 726
EcoT14I CCWWGG 3 cut(s) 410, 589, 996
ErhI CCWWGG 3 cut(s) 410, 589, 996
FaeI CATG 7 cut(s) 17, 94, 115, 268, 414, 928, 1105
FalI AAGNNNNNCTT 4 cut(s) 191, 223, 949, 981
FatI CATG 7 cut(s) 13, 90, 111, 264, 410, 924, 1101
FbaI TGATCA 1 cut(s) 1194
Fnu4HI GCNGC 6 cut(s) 154, 493, 772, 800, 870, 954
FokI GGATG 2 cut(s) 947, 1178
Fsp4HI GCNGC 6 cut(s) 154, 493, 772, 800, 870, 954
FspBI CTAG 5 cut(s) 359, 518, 531, 590, 816
GlaI GCGC 1 cut(s) 206
GluI GCNGC 6 cut(s) 154, 493, 772, 800, 870, 954
GsuI CTGGAG 2 cut(s) 57, 638
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 1 cut(s) 727
HhaI GCGC 1 cut(s) 207
Hin1II CATG 7 cut(s) 17, 94, 115, 268, 414, 928, 1105
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HindIII AAGCTT 2 cut(s) 786, 833
HinfI GANTC 7 cut(s) 261, 331, 415, 534, 544, 668, 702
HphI GGTGA 4 cut(s) 462, 652, 677, 967
Hpy166II GTNNAC 1 cut(s) 1144
Hpy188I TCNGA 6 cut(s) 420, 682, 781, 1081, 1194, 1308
Hpy188III TCNNGA 6 cut(s) 265, 531, 617, 880, 1043, 1102
Hpy8I GTNNAC 1 cut(s) 1144
HpyAV CCTTC 3 cut(s) 753, 788, 975
HpyCH4III ACNGT 1 cut(s) 1290
HpyCH4IV ACGT 2 cut(s) 1110, 1150
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 104, 805
HpyF3I CTNAG 6 cut(s) 325, 575, 681, 1053, 1204, 1329
HpySE526I ACGT 2 cut(s) 1110, 1150
Hsp92II CATG 7 cut(s) 17, 94, 115, 268, 414, 928, 1105
HspAI GCGC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 1194
Kzo9I GATC 5 cut(s) 279, 342, 585, 882, 1194
Lsp1109I GCAGC 5 cut(s) 165, 783, 811, 856, 940
LweI GCATC 4 cut(s) 226, 399, 969, 1184
MaeI CTAG 5 cut(s) 359, 518, 531, 590, 816
MaeII ACGT 2 cut(s) 1110, 1150
MaeIII GTNAC 3 cut(s) 40, 226, 468
MalI GATC 5 cut(s) 281, 344, 587, 884, 1196
MboI GATC 5 cut(s) 279, 342, 585, 882, 1194
MboII GAAGA 5 cut(s) 174, 287, 838, 964, 1120
MluCI AATT 4 cut(s) 316, 348, 434, 1153
MmeI TCCRAC 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 206
MseI TTAA 4 cut(s) 347, 696, 717, 767
MslI CAYNNNNRTG 2 cut(s) 89, 269
MspA1I CMGCKG 1 cut(s) 249
Mva1269I GAATGC 2 cut(s) 371, 1063
MwoI GCNNNNNNNGC 3 cut(s) 59, 104, 805
NcoI CCATGG 1 cut(s) 410
NdeII GATC 5 cut(s) 279, 342, 585, 882, 1194
NlaIII CATG 7 cut(s) 17, 94, 115, 268, 414, 928, 1105
NmuCI GTSAC 1 cut(s) 468
PagI TCATGA 2 cut(s) 264, 1101
PaqCI CACCTGC 1 cut(s) 799
PctI GAATGC 2 cut(s) 371, 1063
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 7 cut(s) 261, 331, 415, 534, 544, 668, 702
PkrI GCNGC 6 cut(s) 155, 494, 773, 801, 871, 955
Ppu21I YACGTR 1 cut(s) 1151
PsiI TTATAA 1 cut(s) 1089
Psp1406I AACGTT 1 cut(s) 1110
PspPI GGNCC 2 cut(s) 726, 1251
PstI CTGCAG 2 cut(s) 55, 64
PstNI CAGNNNCTG 2 cut(s) 50, 59
PvuII CAGCTG 1 cut(s) 249
RsaI GTAC 3 cut(s) 253, 456, 1277
RsaNI GTAC 3 cut(s) 252, 455, 1276
RseI CAYNNNNRTG 2 cut(s) 89, 269
SaqAI TTAA 4 cut(s) 347, 696, 717, 767
SatI GCNGC 6 cut(s) 154, 493, 772, 800, 870, 954
Sau3AI GATC 5 cut(s) 279, 342, 585, 882, 1194
Sau96I GGNCC 2 cut(s) 726, 1251
SfaNI GCATC 4 cut(s) 226, 399, 969, 1184
SfcI CTRYAG 2 cut(s) 51, 60
SinI GGWCC 1 cut(s) 1251
SmiMI CAYNNNNRTG 2 cut(s) 89, 269
SmlI CTYRAG 1 cut(s) 1041
SmoI CTYRAG 1 cut(s) 1041
SnaBI TACGTA 1 cut(s) 1151
SpeI ACTAGT 1 cut(s) 358
Sse9I AATT 4 cut(s) 316, 348, 434, 1153
SsiI CCGC 2 cut(s) 255, 492
SspMI CTAG 5 cut(s) 359, 518, 531, 590, 816
StyI CCWWGG 3 cut(s) 410, 589, 996
TaaI ACNGT 1 cut(s) 1290
TaiI ACGT 2 cut(s) 1113, 1153
TaqI TCGA 3 cut(s) 190, 537, 654
TasI AATT 4 cut(s) 316, 348, 434, 1153
TatI WGTACW 1 cut(s) 454
TauI GCSGC 1 cut(s) 495
TfiI GAWTC 7 cut(s) 261, 331, 415, 534, 544, 668, 702
Tru1I TTAA 4 cut(s) 347, 696, 717, 767
Tru9I TTAA 4 cut(s) 347, 696, 717, 767
TscAI CASTG 3 cut(s) 223, 490, 1293
TseFI GTSAC 1 cut(s) 468
TseI GCWGC 5 cut(s) 153, 771, 799, 869, 953
Tsp45I GTSAC 1 cut(s) 468
TspDTI ATGAA 5 cut(s) 95, 354, 957, 1090, 1118
TspRI CASTG 3 cut(s) 223, 490, 1293
VpaK11BI GGWCC 1 cut(s) 1251
XapI RAATTY 1 cut(s) 434
XbaI TCTAGA 1 cut(s) 530
XmaJI CCTAGG 1 cut(s) 589
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 5 cut(s) 359, 518, 531, 590, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.