FvH4_3g08240

Apoptosis inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004487
Physical Location & Seq
Reverse (-)
4827825 .. 4834957
7133 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08240.t1

Sequence Viewer

Length: 1659 bp
ATGGCGGCTGACCCCTCCGACGAGGCCAAGCACATCGAGAAGCTCTACGAGTTCGGCGAGCGCCTCAATGAGTCCAAGGACAAGTCCCAGAACGTGGAGGACTACCAGGGGATTATAAGTGCGGCAAAGACGAGCATGAAGGCCAAGCAGCTCGCTGCACAGCTTATTCCCAGGTTCTTCAAGTTCTTCCCCGCGCTTTCGTCGCCGGCGATTGACACTCACCTTGATTTGATTGAAGAAGAAGAGCTTGGGGTTCGAGTGCAAGCTATTCGGGGGCTTCCCCTTTTCTGCAAGGACACCCCTGAACACATCTCGAAGATTGTTGACATTCTTGTGCAACTCCTTCACGCTGATGAGTTTGTGGAGCGTGATGCTGTGCATAAGGCTCTTATGGCTCTTTTGAGGCAGGATGTGAAAGCTTCTTTGACAGCCTTGTTCAAGCACATTGGAAGTGTTGATGAACCAAGCCAAGATGATATTATTCGTGAGAAAGTCCTAAGCTTCATCAGAGATAAGGTATTTCCTATTAAAGCTGAAGTTTTGAAGCCTCAGGAGGAAATGGAGAGGCATATTACTGACTTAATAAAAAAGAGTTTAGAAGATGTAACTGGAGCAGAATTTAGAATGTTCATGGACTTCTTAAAAGGTTTGAGCATCTTTGGGGACAAAGCTCTGAAAGAACGGATGGTAGAACTTATTGGAATTATTGAAGGACAAGCTGATTTAGATGCTCAATTCAATGTTTCAGACGCAGACCATATTGACAGGTTGATATCTTGCCTATTTATGGCTCTGCCATTTATTGTGAGAGGGGCATCAAGCAGCAAATTCCTCAACTATCTGAACAAACACATCCTTCCTGTTTCGGATAAGCTTCCTGATGAACGAAGGCTAGATTTGCTCAAGGCCCTTGCAGAAGTTGCACCATTCACCACACCACAAGATTCACGTCAGATTCTTCCTTCTGTGGTTCAGCTGTTAAAGAAAAACATGCCCCGGAGAAAGACTGGAGAGGAGTTCAATTTCACTTATGTCGAATGCTTGTTGTACACATTTCACCATCTTGCTCACAAGGTTCCAAATGCTACCAACAGTTTATGCGGTTACAAGATAGTAACTGGACAACCATCGGATAGGCTTGGTGAGGACTTCACAGAACAGTATAAGGAGTTCACAGAGAGGTTGAGTTACGTGGAGGAGTTAACTAGGGCTACCATGAAGAAATTAACACAGGGGATGGCCGAAAAGAACAAGGCGATGGCAGCTGCAAAATCTGATGAAGCAAAGGATACCATTAAAACTGAAAAGCAGAATACTACAACTGGGTTGCGAACCTGCAACAACATATTGGCAATGACAAAGGCACTGCATTCAAAAGCACCTTCGTTTATTGGTGACAAGAGCATCAACCTATCTTGGAAAGAAGTGCCAAAACCTGCTGTGACTTCAAGCACACCTGCCACAGGTGGAAAGCGACCTGGTAGTGCTGCCAATGGATCTGGCAATCCTTCAAAGAAGGGCCGTGGAGCTGGTGGAATACAACATCAGCTTGTTAATAGAGCATTTGAAGGGTTATCTCATGGTGGAAGGAGTGGCGGGACAAGGGGCAGAGGCAGAGGGTGGGGTGGACGTGGAAGAGGAAGGGGTTACAACAGATAG

Protein Analysis

553

Amino Acids

61.41

Weight (kDa)

8.95

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
API5 PF05918 12 - 549 3.2e-246 Apoptosis inhibitory protein 5 (API5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 116
AarI CACCTGC 1 cut(s) 1465
Acc36I ACCTGC 3 cut(s) 1343, 1444, 1465
AccB7I CCANNNNNTGG 1 cut(s) 94
AccII CGCG 1 cut(s) 194
AciI CCGC 5 cut(s) 5, 122, 192, 1101, 1596
AclWI GGATC 1 cut(s) 1504
AcoI YGGCCR 1 cut(s) 1239
AcsI RAATTY 2 cut(s) 617, 827
AcuI CTGAAG 1 cut(s) 555
AfaI GTAC 1 cut(s) 1049
AfiI CCNNNNNNNGG 3 cut(s) 94, 787, 1463
AjiI CACGTC 2 cut(s) 950, 1631
AjnI CCWGG 3 cut(s) 105, 170, 1477
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AlwI GGATC 1 cut(s) 1504
AoxI GGCC 5 cut(s) 24, 141, 906, 1239, 1519
ApeKI GCWGC 6 cut(s) 148, 155, 822, 1262, 1265, 1487
ApoI RAATTY 2 cut(s) 617, 827
AspLEI GCGC 2 cut(s) 63, 196
AspS9I GGNCC 2 cut(s) 907, 1519
AsuC2I CCSGG 1 cut(s) 997
AsuHPI GGTGA 5 cut(s) 212, 922, 1049, 1154, 1406
AxyI CCTNAGG 1 cut(s) 549
BbvI GCAGC 6 cut(s) 142, 160, 834, 1252, 1274, 1474
BccI CCATC 5 cut(s) 679, 1068, 1135, 1231, 1252
BceAI ACGGC 1 cut(s) 1506
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 3 cut(s) 107, 172, 1479
BciVI GTATCC 1 cut(s) 1282
BcnI CCSGG 1 cut(s) 997
BfaI CTAG 2 cut(s) 893, 1206
BfoI RGCGCY 1 cut(s) 64
BfuAI ACCTGC 3 cut(s) 1343, 1444, 1465
BfuI GTATCC 1 cut(s) 1282
BisI GCNGC 8 cut(s) 6, 123, 149, 156, 823, 1263, 1266, 1488
BlsI GCNGC 8 cut(s) 7, 124, 150, 157, 824, 1264, 1267, 1489
Bme1390I CCNGG 4 cut(s) 107, 172, 997, 1479
BmgBI CACGTC 2 cut(s) 950, 1631
BmgT120I GGNCC 2 cut(s) 907, 1519
BmiI GGNNCC 1 cut(s) 1077
BmrFI CCNGG 4 cut(s) 107, 172, 997, 1479
BmrI ACTGGG 1 cut(s) 1332
BmsI GCATC 5 cut(s) 361, 663, 718, 824, 1413
BmuI ACTGGG 1 cut(s) 1332
BpmI CTGGAG 2 cut(s) 630, 1029
Bpu10I CCTNAGC 1 cut(s) 497
BpuEI CTTGAG 1 cut(s) 887
BpuMI CCSGG 1 cut(s) 997
BsaAI YACGTR 1 cut(s) 1192
BsaJI CCNNGG 5 cut(s) 75, 106, 170, 995, 1522
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 787, 1463
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 4 cut(s) 613, 1012, 1123, 1327
Bse21I CCTNAGG 1 cut(s) 549
Bse3DI GCAATG 1 cut(s) 1359
BseBI CCWGG 3 cut(s) 107, 172, 1479
BseDI CCNNGG 5 cut(s) 75, 106, 170, 995, 1522
BseGI GGATG 4 cut(s) 415, 690, 852, 1242
BseLI CCNNNNNNNGG 3 cut(s) 94, 787, 1463
BseMI GCAATG 1 cut(s) 1359
BseMII CTCAG 1 cut(s) 563
BseNI ACTGG 4 cut(s) 613, 1012, 1123, 1327
BseRI GAGGAG 2 cut(s) 1028, 1211
BseXI GCAGC 6 cut(s) 142, 160, 834, 1252, 1274, 1474
BsgI GTGCAG 1 cut(s) 141
Bsh1236I CGCG 1 cut(s) 194
BshFI GGCC 5 cut(s) 26, 143, 908, 1241, 1521
BsiSI CCGG 2 cut(s) 206, 997
BslFI GGGAC 3 cut(s) 70, 677, 1612
BslI CCNNNNNNNGG 3 cut(s) 94, 787, 1463
BsmFI GGGAC 3 cut(s) 70, 677, 1612
BsmI GAATGC 2 cut(s) 1043, 1369
BsnI GGCC 5 cut(s) 26, 143, 908, 1241, 1521
Bsp1407I TGTACA 1 cut(s) 1047
Bsp143I GATC 1 cut(s) 1496
BspACI CCGC 5 cut(s) 5, 122, 192, 1101, 1596
BspANI GGCC 5 cut(s) 26, 143, 908, 1241, 1521
BspCNI CTCAG 1 cut(s) 562
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 1077
BspMI ACCTGC 3 cut(s) 1343, 1444, 1465
BspPI GGATC 1 cut(s) 1504
BspQI GCTCTTC 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 1359
BsrFI RCCGGY 1 cut(s) 205
BsrGI TGTACA 1 cut(s) 1047
BsrI ACTGG 4 cut(s) 613, 1012, 1123, 1327
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 5 cut(s) 75, 106, 170, 995, 1522
BssMI GATC 1 cut(s) 1496
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 3 cut(s) 107, 172, 1479
Bst4CI ACNGT 2 cut(s) 1094, 1161
Bst6I CTCTTC 2 cut(s) 237, 1630
BstAPI GCANNNNNTGC 1 cut(s) 920
BstAUI TGTACA 1 cut(s) 1047
BstBAI YACGTR 1 cut(s) 1192
BstC8I GCNNGC 4 cut(s) 59, 153, 207, 264
BstDEI CTNAG 2 cut(s) 497, 549
BstDSI CCRYGG 1 cut(s) 1522
BstENI CCTNNNNNAGG 1 cut(s) 1461
BstF5I GGATG 4 cut(s) 415, 690, 852, 1242
BstFNI CGCG 1 cut(s) 194
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 2 cut(s) 63, 196
BstKTI GATC 1 cut(s) 1499
BstMBI GATC 1 cut(s) 1496
BstMWI GCNNNNNNNGC 5 cut(s) 202, 392, 898, 920, 1262
BstNI CCWGG 3 cut(s) 107, 172, 1479
BstNSI RCATGY 1 cut(s) 994
BstSCI CCNGG 4 cut(s) 105, 170, 995, 1477
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 6 cut(s) 142, 160, 834, 1252, 1274, 1474
BstX2I RGATCY 1 cut(s) 1496
BstYI RGATCY 1 cut(s) 1496
Bsu36I CCTNAGG 1 cut(s) 549
BsuI GTATCC 1 cut(s) 1282
BsuRI GGCC 5 cut(s) 26, 143, 908, 1241, 1521
BtgI CCRYGG 1 cut(s) 1522
BtgZI GCGATG 1 cut(s) 1271
BtrI CACGTC 2 cut(s) 950, 1631
BtsCI GGATG 4 cut(s) 415, 690, 852, 1242
BtsI GCAGTG 1 cut(s) 1364
BtsIMutI CAGTG 1 cut(s) 1364
BveI ACCTGC 3 cut(s) 1343, 1444, 1465
Cac8I GCNNGC 4 cut(s) 59, 153, 207, 264
CfoI GCGC 2 cut(s) 63, 196
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 2 cut(s) 907, 1519
CseI GACGC 1 cut(s) 758
CsiI ACCWGGT 1 cut(s) 1477
Csp6I GTAC 1 cut(s) 1048
CviAII CATG 5 cut(s) 136, 631, 991, 1216, 1580
CviQI GTAC 1 cut(s) 1048
DdeI CTNAG 2 cut(s) 497, 549
DpnI GATC 1 cut(s) 1498
DpnII GATC 1 cut(s) 1496
EaeI YGGCCR 1 cut(s) 1239
Eam1104I CTCTTC 2 cut(s) 237, 1630
EarI CTCTTC 2 cut(s) 237, 1630
Eco130I CCWWGG 1 cut(s) 75
Eco32I GATATC 1 cut(s) 774
Eco57I CTGAAG 1 cut(s) 555
Eco81I CCTNAGG 1 cut(s) 549
EcoNI CCTNNNNNAGG 1 cut(s) 1461
EcoO109I RGGNCCY 1 cut(s) 907
EcoRII CCWGG 3 cut(s) 105, 170, 1477
EcoRV GATATC 1 cut(s) 774
EcoT14I CCWWGG 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 5 cut(s) 139, 634, 994, 1219, 1583
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 3 cut(s) 70, 677, 1612
FatI CATG 5 cut(s) 135, 630, 990, 1215, 1579
FauI CCCGC 2 cut(s) 199, 1589
Fnu4HI GCNGC 8 cut(s) 6, 123, 149, 156, 823, 1263, 1266, 1488
FokI GGATG 4 cut(s) 422, 697, 839, 1249
Fsp4HI GCNGC 8 cut(s) 6, 123, 149, 156, 823, 1263, 1266, 1488
FspBI CTAG 2 cut(s) 893, 1206
GlaI GCGC 2 cut(s) 62, 195
GluI GCNGC 8 cut(s) 6, 123, 149, 156, 823, 1263, 1266, 1488
GsuI CTGGAG 2 cut(s) 630, 1029
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 5 cut(s) 26, 143, 908, 1241, 1521
HapII CCGG 2 cut(s) 206, 997
HgaI GACGC 1 cut(s) 758
HhaI GCGC 2 cut(s) 63, 196
Hin1II CATG 5 cut(s) 139, 634, 994, 1219, 1583
Hin6I GCGC 2 cut(s) 61, 194
HinP1I GCGC 2 cut(s) 61, 194
HincII GTYRAC 2 cut(s) 325, 1203
HindII GTYRAC 2 cut(s) 325, 1203
HindIII AAGCTT 3 cut(s) 417, 499, 872
HinfI GANTC 3 cut(s) 71, 944, 955
HpaI GTTAAC 1 cut(s) 1203
HpaII CCGG 2 cut(s) 206, 997
HphI GGTGA 5 cut(s) 212, 922, 1049, 1154, 1406
Hpy166II GTNNAC 5 cut(s) 325, 1050, 1173, 1203, 1628
Hpy188I TCNGA 9 cut(s) 19, 509, 675, 748, 843, 868, 954, 1132, 1276
Hpy188III TCNNGA 5 cut(s) 37, 313, 485, 551, 878
Hpy8I GTNNAC 5 cut(s) 325, 1050, 1173, 1203, 1628
Hpy99I CGWCG 2 cut(s) 23, 205
HpyCH4III ACNGT 2 cut(s) 1094, 1161
HpyCH4IV ACGT 4 cut(s) 93, 949, 1191, 1630
HpyF10VI GCNNNNNNNGC 5 cut(s) 202, 392, 898, 920, 1262
HpyF3I CTNAG 2 cut(s) 497, 549
HpySE526I ACGT 4 cut(s) 93, 949, 1191, 1630
Hsp92II CATG 5 cut(s) 139, 634, 994, 1219, 1583
HspAI GCGC 2 cut(s) 61, 194
KroI GCCGGC 1 cut(s) 205
KroNI GCCGGC 1 cut(s) 207
KspAI GTTAAC 1 cut(s) 1203
Kzo9I GATC 1 cut(s) 1496
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 3 cut(s) 364, 611, 1526
Lsp1109I GCAGC 6 cut(s) 142, 160, 834, 1252, 1274, 1474
LweI GCATC 5 cut(s) 361, 663, 718, 824, 1413
MabI ACCWGGT 1 cut(s) 1477
MaeI CTAG 2 cut(s) 893, 1206
MaeII ACGT 4 cut(s) 93, 949, 1191, 1630
MaeIII GTNAC 7 cut(s) 604, 1103, 1114, 1187, 1394, 1441, 1646
MalI GATC 1 cut(s) 1498
MboI GATC 1 cut(s) 1496
MflI RGATCY 1 cut(s) 1496
MluCI AATT 6 cut(s) 617, 702, 734, 827, 1021, 1223
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 1 cut(s) 42
MreI CGCCGGCG 1 cut(s) 205
MroNI GCCGGC 1 cut(s) 205
MseI TTAA 8 cut(s) 528, 581, 641, 980, 1202, 1226, 1296, 1554
MslI CAYNNNNRTG 2 cut(s) 332, 351
MspA1I CMGCKG 2 cut(s) 976, 1265
MspI CCGG 2 cut(s) 206, 997
MspR9I CCNGG 4 cut(s) 107, 172, 997, 1479
Mva1269I GAATGC 2 cut(s) 1043, 1369
MvaI CCWGG 3 cut(s) 107, 172, 1479
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 5 cut(s) 202, 392, 898, 920, 1262
NaeI GCCGGC 1 cut(s) 207
NciI CCSGG 1 cut(s) 997
NdeII GATC 1 cut(s) 1496
NgoMIV GCCGGC 1 cut(s) 205
NlaIII CATG 5 cut(s) 139, 634, 994, 1219, 1583
NlaIV GGNNCC 1 cut(s) 1077
NmuCI GTSAC 2 cut(s) 1394, 1441
NspI RCATGY 1 cut(s) 994
PaqCI CACCTGC 1 cut(s) 1465
PciSI GCTCTTC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 206
PctI GAATGC 2 cut(s) 1043, 1369
PdiI GCCGGC 1 cut(s) 207
PfeI GAWTC 2 cut(s) 944, 955
PflMI CCANNNNNTGG 1 cut(s) 94
PkrI GCNGC 8 cut(s) 7, 124, 150, 157, 824, 1264, 1267, 1489
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Ppu21I YACGTR 1 cut(s) 1192
PsiI TTATAA 1 cut(s) 116
Psp6I CCWGG 3 cut(s) 105, 170, 1477
PspGI CCWGG 3 cut(s) 105, 170, 1477
PspN4I GGNNCC 1 cut(s) 1077
PspPI GGNCC 2 cut(s) 907, 1519
PsrI GAACNNNNNNTAC 2 cut(s) 1154, 1186
PsuI RGATCY 1 cut(s) 1496
PvuII CAGCTG 2 cut(s) 976, 1265
RsaI GTAC 1 cut(s) 1049
RsaNI GTAC 1 cut(s) 1048
RseI CAYNNNNRTG 2 cut(s) 332, 351
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 8 cut(s) 528, 581, 641, 980, 1202, 1226, 1296, 1554
SatI GCNGC 8 cut(s) 6, 123, 149, 156, 823, 1263, 1266, 1488
Sau3AI GATC 1 cut(s) 1496
Sau96I GGNCC 2 cut(s) 907, 1519
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 4 cut(s) 107, 172, 997, 1479
SexAI ACCWGGT 1 cut(s) 1477
SfaNI GCATC 5 cut(s) 361, 663, 718, 824, 1413
SgrAI CRCCGGYG 1 cut(s) 205
SmiMI CAYNNNNRTG 2 cut(s) 332, 351
SmlI CTYRAG 1 cut(s) 902
SmoI CTYRAG 1 cut(s) 902
Sse9I AATT 6 cut(s) 617, 702, 734, 827, 1021, 1223
SsiI CCGC 5 cut(s) 5, 122, 192, 1101, 1596
SspMI CTAG 2 cut(s) 893, 1206
StyD4I CCNGG 4 cut(s) 105, 170, 995, 1477
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 2 cut(s) 1094, 1161
TaiI ACGT 4 cut(s) 96, 952, 1194, 1633
TaqI TCGA 4 cut(s) 36, 256, 314, 1035
TasI AATT 6 cut(s) 617, 702, 734, 827, 1021, 1223
TatI WGTACW 1 cut(s) 1047
TauI GCSGC 2 cut(s) 8, 125
TfiI GAWTC 2 cut(s) 944, 955
Tru1I TTAA 8 cut(s) 528, 581, 641, 980, 1202, 1226, 1296, 1554
Tru9I TTAA 8 cut(s) 528, 581, 641, 980, 1202, 1226, 1296, 1554
TscAI CASTG 1 cut(s) 1371
TseFI GTSAC 2 cut(s) 1394, 1441
TseI GCWGC 6 cut(s) 148, 155, 822, 1262, 1265, 1487
Tsp45I GTSAC 2 cut(s) 1394, 1441
TspDTI ATGAA 7 cut(s) 152, 474, 493, 619, 897, 1232, 1293
TspGWI ACGGA 1 cut(s) 697
TspRI CASTG 1 cut(s) 1371
Van91I CCANNNNNTGG 1 cut(s) 94
XagI CCTNNNNNAGG 1 cut(s) 1461
XapI RAATTY 2 cut(s) 617, 827
XceI RCATGY 1 cut(s) 994
XspI CTAG 2 cut(s) 893, 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.