Rh5DG073800

Apoptosis inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004487
Physical Location & Seq
Forward (+)
6448679 .. 6455952
7274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG073800.1

Sequence Viewer

Length: 1212 bp
ATGGCGGCACCAGCAGCTGACCCCTCCGACGAGGCCAAGCACATCGAGAAGCTCTACGAGTTCGGCGAGCGACTCAACGAGTCCAAGGACAAATCCCAGAATGTCGAGGACTACGAGGGGATTATCAGTGCGGCAAAGACGAGCATGAAGGCCAAGCAGCTGGCTGCACAGCTTATTCCCAGGTTCTTCAAGTTCTTCCCCGAGCTTTCGTCGCCGGCGATAGATACTCACCTTGATTTGATTGAAGAAGAAGAGCTTGGGGTTCGAGTGCAAGCTATTCGGGGGCTTCCCCTTTTCTGCAAGGACACCCCTGAACACATCTCGAAGATTGTAGACATTCTTGTGCAACTCCTGCAAGCTGATGAGTTTGTGGAGCGTGATGCTGTGCATAAGGCTCTTATGGCCCTTTTGAGGCAGGATGTGAAAGCTTCTTTGACAGCCTTGTTCAAGCACATTGGAAGTGTTGATGAACCTACCCAAGAGGATGTTATTCGTGAGAAAGTCCTAAGCTTTATCAGAGATAAGGTATTTCCTATTAAAGCTGAAGTTTTGAAGCCTCAGGAGGAGATGGAGAGGCATATTACTGACTTGATAAAAAAGAGTTTAGAAGATGTAACTGGAGCAGAGTTTAGAATGTTTATGGACTTTTTAAAAGGTCTGAGCATCTTTGGGGAGAAAGCTCCGAAAGAACGGATGGTAGAACTTATTGGAATTATTGAAGGACAAGCCGATTTAGATGCTCAATTCAATGTATCAGATGCAGACCATATCGACAGGTTGATATCTTGCCTATTTATGGCTCTGCCATTTATTGTGAGAGGTGCATCAAGCAGCAAATTCCTCAACTATCTGAACAAACACATCCTTCCTGTTTCGGATAAGCTTCCTGATGAACGAAGGCTAGATTTGCTCAAGGCCCTTGCAGAAGTTGCACCATTCACCACACCACAAGATTCACGTCAGATTCTTCCTTCTGTGGTTCAGCTGTTAAAGAAAAACATGCCTCGGAGAAAGACTGGAGAGGAGTTCAACTTCACTTATGTCGAATGCTTGTTGTACACATTTCACCATCTTGCTCACAAGGTTCCAAATGCTACCAACAGTTTATGCGGTTACAAGATAGTAACCGGACAACCATCAGATAGGCTTGGTGAGGACTTTACAGAGCAGTATAAGGAATTCACAGAGAGGTATCTACCCTTTTATGTGTAG

Protein Analysis

403

Amino Acids

45.91

Weight (kDa)

5.44

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
API5 PF05918 15 - 397 1.1e-196 Apoptosis inhibitory protein 5 (API5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AccI GTMKAC 1 cut(s) 333
AciI CCGC 3 cut(s) 5, 131, 1110
AcsI RAATTY 2 cut(s) 836, 1178
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 1 cut(s) 1058
AfiI CCNNNNNNNGG 2 cut(s) 411, 796
AgsI TTSAA 7 cut(s) 190, 245, 448, 553, 719, 748, 1030
AjiI CACGTC 1 cut(s) 959
AjnI CCWGG 1 cut(s) 179
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 4 cut(s) 33, 150, 402, 915
ApeKI GCWGC 4 cut(s) 14, 157, 164, 831
ApoI RAATTY 2 cut(s) 836, 1178
AspS9I GGNCC 2 cut(s) 403, 916
AsuHPI GGTGA 4 cut(s) 221, 931, 1058, 1163
AvaI CYCGRG 1 cut(s) 200
AxyI CCTNAGG 1 cut(s) 558
BanI GGYRCC 1 cut(s) 7
BbvI GCAGC 4 cut(s) 26, 151, 169, 843
BccI CCATC 4 cut(s) 562, 688, 1077, 1144
BcgI CGANNNNNNTGC 4 cut(s) 260, 294, 719, 753
BciT130I CCWGG 1 cut(s) 181
BfaI CTAG 1 cut(s) 902
BisI GCNGC 6 cut(s) 6, 15, 132, 158, 165, 832
BlsI GCNGC 6 cut(s) 7, 16, 133, 159, 166, 833
Bme1390I CCNGG 1 cut(s) 181
BmeT110I CYCGRG 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 959
BmgT120I GGNCC 2 cut(s) 403, 916
BmiI GGNNCC 2 cut(s) 9, 1086
BmrFI CCNGG 1 cut(s) 181
BmsI GCATC 5 cut(s) 370, 672, 727, 748, 833
BpmI CTGGAG 2 cut(s) 639, 1038
Bpu10I CCTNAGC 1 cut(s) 506
BpuEI CTTGAG 1 cut(s) 896
BsaJI CCNNGG 3 cut(s) 84, 179, 1004
BsaWI WCCGGW 1 cut(s) 1127
Bsc4I CCNNNNNNNGG 2 cut(s) 411, 796
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 622, 1021
Bse21I CCTNAGG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 181
BseDI CCNNGG 3 cut(s) 84, 179, 1004
BseGI GGATG 4 cut(s) 424, 490, 699, 861
BseLI CCNNNNNNNGG 2 cut(s) 411, 796
BseMII CTCAG 2 cut(s) 572, 650
BseNI ACTGG 2 cut(s) 622, 1021
BseRI GAGGAG 2 cut(s) 578, 1037
BseXI GCAGC 4 cut(s) 26, 151, 169, 843
BsgI GTGCAG 1 cut(s) 150
BshFI GGCC 4 cut(s) 35, 152, 404, 917
BshNI GGYRCC 1 cut(s) 7
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 2 cut(s) 215, 1128
BslI CCNNNNNNNGG 2 cut(s) 411, 796
BsmI GAATGC 1 cut(s) 1052
BsnI GGCC 4 cut(s) 35, 152, 404, 917
BsoBI CYCGRG 1 cut(s) 200
Bsp1407I TGTACA 1 cut(s) 1056
BspACI CCGC 3 cut(s) 5, 131, 1110
BspANI GGCC 4 cut(s) 35, 152, 404, 917
BspCNI CTCAG 2 cut(s) 571, 651
BspLI GGNNCC 2 cut(s) 9, 1086
BspQI GCTCTTC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 7
BsrFI RCCGGY 1 cut(s) 214
BsrGI TGTACA 1 cut(s) 1056
BsrI ACTGG 2 cut(s) 622, 1021
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 3 cut(s) 84, 179, 1004
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 181
Bst4CI ACNGT 1 cut(s) 1103
Bst6I CTCTTC 1 cut(s) 246
BstAPI GCANNNNNTGC 2 cut(s) 352, 929
BstAUI TGTACA 1 cut(s) 1056
BstC8I GCNNGC 5 cut(s) 68, 162, 216, 273, 357
BstDEI CTNAG 3 cut(s) 506, 558, 659
BstF5I GGATG 4 cut(s) 424, 490, 699, 861
BstMWI GCNNNNNNNGC 7 cut(s) 11, 14, 211, 352, 401, 907, 929
BstNI CCWGG 1 cut(s) 181
BstNSI RCATGY 1 cut(s) 1003
BstSCI CCNGG 1 cut(s) 179
BstV1I GCAGC 4 cut(s) 26, 151, 169, 843
BstXI CCANNNNNNTGG 1 cut(s) 160
Bsu36I CCTNAGG 1 cut(s) 558
BsuRI GGCC 4 cut(s) 35, 152, 404, 917
BtrI CACGTC 1 cut(s) 959
BtsCI GGATG 4 cut(s) 424, 490, 699, 861
BtsIMutI CAGTG 1 cut(s) 133
Cac8I GCNNGC 5 cut(s) 68, 162, 216, 273, 357
CaiI CAGNNNCTG 1 cut(s) 17
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 403, 916
Csp6I GTAC 1 cut(s) 1057
CviAII CATG 2 cut(s) 145, 1000
CviQI GTAC 1 cut(s) 1057
DdeI CTNAG 3 cut(s) 506, 558, 659
DraI TTTAAA 1 cut(s) 651
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 84
Eco32I GATATC 1 cut(s) 783
Eco57I CTGAAG 1 cut(s) 564
Eco81I CCTNAGG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 916
EcoRI GAATTC 1 cut(s) 1178
EcoRII CCWGG 1 cut(s) 179
EcoRV GATATC 1 cut(s) 783
EcoT14I CCWWGG 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 2 cut(s) 148, 1003
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 2 cut(s) 144, 999
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 6 cut(s) 6, 15, 132, 158, 165, 832
FokI GGATG 4 cut(s) 431, 497, 706, 848
Fsp4HI GCNGC 6 cut(s) 6, 15, 132, 158, 165, 832
FspBI CTAG 1 cut(s) 902
GluI GCNGC 6 cut(s) 6, 15, 132, 158, 165, 832
GsuI CTGGAG 2 cut(s) 639, 1038
HaeIII GGCC 4 cut(s) 35, 152, 404, 917
HapII CCGG 2 cut(s) 215, 1128
Hin1II CATG 2 cut(s) 148, 1003
HindIII AAGCTT 3 cut(s) 426, 508, 881
HinfI GANTC 4 cut(s) 72, 80, 953, 964
HpaII CCGG 2 cut(s) 215, 1128
HphI GGTGA 4 cut(s) 221, 931, 1058, 1163
Hpy166II GTNNAC 2 cut(s) 334, 1059
Hpy188III TCNNGA 5 cut(s) 46, 322, 494, 560, 887
Hpy8I GTNNAC 2 cut(s) 334, 1059
Hpy99I CGWCG 2 cut(s) 32, 214
HpyAV CCTTC 5 cut(s) 142, 713, 875, 891, 981
HpyCH4III ACNGT 1 cut(s) 1103
HpyCH4IV ACGT 1 cut(s) 958
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 14, 211, 352, 401, 907, 929
HpyF3I CTNAG 3 cut(s) 506, 558, 659
HpySE526I ACGT 1 cut(s) 958
Hsp92II CATG 2 cut(s) 148, 1003
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 3 cut(s) 373, 620, 685
Lsp1109I GCAGC 4 cut(s) 26, 151, 169, 843
LweI GCATC 5 cut(s) 370, 672, 727, 748, 833
MaeI CTAG 1 cut(s) 902
MaeII ACGT 1 cut(s) 958
MaeIII GTNAC 3 cut(s) 613, 1112, 1123
MboII GAAGA 8 cut(s) 178, 187, 257, 260, 263, 337, 620, 959
MluCI AATT 4 cut(s) 711, 743, 836, 1178
MlyI GAGTC 2 cut(s) 66, 89
MmeI TCCRAC 1 cut(s) 51
MreI CGCCGGCG 1 cut(s) 214
MroNI GCCGGC 1 cut(s) 214
MseI TTAA 3 cut(s) 537, 650, 989
MslI CAYNNNNRTG 1 cut(s) 341
MspA1I CMGCKG 3 cut(s) 17, 160, 985
MspI CCGG 2 cut(s) 215, 1128
MspR9I CCNGG 1 cut(s) 181
Mva1269I GAATGC 1 cut(s) 1052
MvaI CCWGG 1 cut(s) 181
MwoI GCNNNNNNNGC 7 cut(s) 11, 14, 211, 352, 401, 907, 929
NaeI GCCGGC 1 cut(s) 216
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 2 cut(s) 148, 1003
NlaIV GGNNCC 2 cut(s) 9, 1086
NspI RCATGY 1 cut(s) 1003
PciSI GCTCTTC 1 cut(s) 246
PcsI WCGNNNNNNNCGW 3 cut(s) 63, 111, 215
PctI GAATGC 1 cut(s) 1052
PdiI GCCGGC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 953, 964
PkrI GCNGC 6 cut(s) 7, 16, 133, 159, 166, 833
PleI GAGTC 2 cut(s) 66, 88
PpsI GAGTC 2 cut(s) 66, 88
Psp6I CCWGG 1 cut(s) 179
PspGI CCWGG 1 cut(s) 179
PspN4I GGNNCC 2 cut(s) 9, 1086
PspPI GGNCC 2 cut(s) 403, 916
PstNI CAGNNNCTG 1 cut(s) 17
PvuII CAGCTG 3 cut(s) 17, 160, 985
RsaI GTAC 1 cut(s) 1058
RsaNI GTAC 1 cut(s) 1057
RseI CAYNNNNRTG 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 3 cut(s) 537, 650, 989
SatI GCNGC 6 cut(s) 6, 15, 132, 158, 165, 832
Sau96I GGNCC 2 cut(s) 403, 916
SchI GAGTC 2 cut(s) 66, 89
ScrFI CCNGG 1 cut(s) 181
SfaNI GCATC 5 cut(s) 370, 672, 727, 748, 833
SgrAI CRCCGGYG 1 cut(s) 214
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
Sse9I AATT 4 cut(s) 711, 743, 836, 1178
SsiI CCGC 3 cut(s) 5, 131, 1110
SspMI CTAG 1 cut(s) 902
StyD4I CCNGG 1 cut(s) 179
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 1 cut(s) 1103
TaiI ACGT 1 cut(s) 961
TaqI TCGA 6 cut(s) 45, 105, 265, 323, 771, 1044
TasI AATT 4 cut(s) 711, 743, 836, 1178
TatI WGTACW 1 cut(s) 1056
TauI GCSGC 2 cut(s) 8, 134
TfiI GAWTC 2 cut(s) 953, 964
Tru1I TTAA 3 cut(s) 537, 650, 989
Tru9I TTAA 3 cut(s) 537, 650, 989
TscAI CASTG 1 cut(s) 133
TseI GCWGC 4 cut(s) 14, 157, 164, 831
TspDTI ATGAA 3 cut(s) 161, 483, 906
TspGWI ACGGA 1 cut(s) 706
TspRI CASTG 1 cut(s) 133
XapI RAATTY 2 cut(s) 836, 1178
XceI RCATGY 1 cut(s) 1003
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.