Rh5AG078400

Apoptosis inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004487
Physical Location & Seq
Forward (+)
6712644 .. 6719916
7273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG078400.1

Sequence Viewer

Length: 1668 bp
ATGGCGGCACCAGCAGCTGACCCCTCCGACGAGGCCAAGCACATCGAGAAGCTCTACGAGTTCGGCGAGCGACTCAACGAGTCCAAGGACAAATCCCAGAATGTCGAGGACTACGAGGGGATTATCAGTGCGGCAAAGACGAGCATGAAGGCCAAGCAGCTGGCTGCACAGCTTATTCCCAGGTTCTTCAAGTTCTTCCCCGAGCTTTCGTCGCCGGCGATAGATACTCACCTTGATTTGATTGAAGAAGAAGAGCTTGGGGTTCGAGTGCAAGCTATTCGGGGGCTTCCCCTTTTCTGCAAGGACACCCCTGAACACATCTCGAAGATTGTAGACATTCTTGTGCAACTCCTGCAAGCTGATGAGTTTGTGGAGCGTGATGCTGTGCATAAGGCTCTTATGGCCCTTTTGAGGCAGGATGTGAAAGCTTCTTTGACAGCCTTGTTCAAGCACATTGGAAGTGTTGATGAACCTACCCAAGAGGATGTTATTCGTGAGAAAGTCCTAAGCTTTATCAGAGATAAGGTATTTCCTATTAAAGCTGAAGTTTTGAAGCCTCAGGAGGAGATGGAGAGGCATATTACTGACTTGATAAAAAAGAGTTTAGAAGATGTAACTGGAGCAGAGTTTAGAATGTTTATGGACTTTTTAAAAGGTCTGAGCATCTTTGGGGAGAAAGCTCCGAAAGAACGGATGGTAGAACTTATTGGAATTATTGAAGGACAAGCCGATTTAGATGCTCAATTCAATGTATCAGATGCAGACCATATCGACAGGTTGATATCTTGCCTATTTATGGCTCTGCCATTTATTGTGAGAGGTGCATCAAGCAGCAAATTCCTCAACTATCTGAACAAACACATCCTTCCTGTTTCGGATAAGCTTCCTGATGAACGAAGGCTAGATTTGCTCAAGGCCCTTGCAGAAGTTGCACCATTCACCACACCACAAGATTCACGTCAGATTCTTCCTTCTGTGGTTCAGCTGTTAAAGAAAAACATGCCTCGGAGAAAGACTGGAGAGGAGTTCAACTTCACTTATGTCGAATGCTTGTTGTACACATTTCACCATCTTGCTCACAAGGTTCCAAATGCTACCAACAGTTTATGCGGTTACAAGATAGTAACCGGACAACCATCAGATAGGCTTGGTGAGGACTTTACAGAGCAGTATAAGGAATTCACAGAGAGGTTGAGTTACGTGGAGGAGTTAACCAGGGCTACCATGAAGAAATTAACCCAAGGAATGGCCGAAAAGAACAAGGCGATGGCAGCTGCAAAATCTGATGAAGCAAAGGACAACATCAAAACTGAAAAGCAGAATACTACAACTGGTTTGCGAACCTGCAATAACATATTGGCAATGACAAAGGCACTGCATTCAAAAACACCTTCATTTATTGGAGACAAGAGCATCAACCTATCTTGGAAAGAAGTGCCAAAACCTTCTGTGCCCTCAAGCACACCAGCCACAGGTGGAAAACGACCTGCTAATGCTGCCAATGGATCTGGCAATCCTTCAAAGAAGGGCCGTGGTGCTGGAGGATTACAACATCAGCTTGTTAATAGAGCATTTGAAGGGTTATCTCATGGCGGAAGGAGTGGCGGGACAAGGGGCCGAGGCAGAGGGTGGGGTGGACGCGGAAGAGGAAGGGGTTACAACAGATAG

Protein Analysis

555

Amino Acids

61.8

Weight (kDa)

8.77

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
API5 PF05918 15 - 552 4.4e-249 Apoptosis inhibitory protein 5 (API5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1352, 1495
AccB1I GGYRCC 1 cut(s) 7
AccB7I CCANNNNNTGG 1 cut(s) 1246
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 1641
AciI CCGC 6 cut(s) 5, 131, 1110, 1593, 1605, 1641
AclWI GGATC 1 cut(s) 1513
AcoI YGGCCR 1 cut(s) 1248
AcsI RAATTY 2 cut(s) 836, 1178
AcuI CTGAAG 1 cut(s) 564
AfaI GTAC 1 cut(s) 1058
AfiI CCNNNNNNNGG 4 cut(s) 411, 796, 1246, 1472
AjiI CACGTC 1 cut(s) 959
AjnI CCWGG 2 cut(s) 179, 1214
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
Alw26I GTCTC 1 cut(s) 1398
AlwI GGATC 1 cut(s) 1513
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 7 cut(s) 33, 150, 402, 915, 1248, 1528, 1615
ApeKI GCWGC 7 cut(s) 14, 157, 164, 831, 1271, 1274, 1496
ApoI RAATTY 2 cut(s) 836, 1178
AspS9I GGNCC 4 cut(s) 403, 916, 1528, 1615
AsuHPI GGTGA 4 cut(s) 221, 931, 1058, 1163
AvaI CYCGRG 1 cut(s) 200
AxyI CCTNAGG 1 cut(s) 558
BaeGI GKGCMC 1 cut(s) 1455
BanI GGYRCC 1 cut(s) 7
BbvI GCAGC 7 cut(s) 26, 151, 169, 843, 1261, 1283, 1483
BccI CCATC 5 cut(s) 562, 688, 1077, 1144, 1261
BceAI ACGGC 1 cut(s) 1515
BcgI CGANNNNNNTGC 4 cut(s) 260, 294, 719, 753
BciT130I CCWGG 2 cut(s) 181, 1216
BcoDI GTCTC 1 cut(s) 1398
BfaI CTAG 1 cut(s) 902
BfuAI ACCTGC 2 cut(s) 1352, 1495
BisI GCNGC 9 cut(s) 6, 15, 132, 158, 165, 832, 1272, 1275, 1497
BlsI GCNGC 9 cut(s) 7, 16, 133, 159, 166, 833, 1273, 1276, 1498
Bme1390I CCNGG 2 cut(s) 181, 1216
BmeT110I CYCGRG 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 959
BmgT120I GGNCC 4 cut(s) 403, 916, 1528, 1615
BmiI GGNNCC 3 cut(s) 9, 1086, 1616
BmrFI CCNGG 2 cut(s) 181, 1216
BmsI GCATC 6 cut(s) 370, 672, 727, 748, 833, 1422
BpmI CTGGAG 3 cut(s) 639, 1038, 1560
Bpu10I CCTNAGC 1 cut(s) 506
BpuEI CTTGAG 2 cut(s) 896, 1441
BsaAI YACGTR 1 cut(s) 1201
BsaJI CCNNGG 7 cut(s) 84, 179, 1004, 1215, 1240, 1531, 1618
BsaWI WCCGGW 1 cut(s) 1127
Bsc4I CCNNNNNNNGG 4 cut(s) 411, 796, 1246, 1472
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 3 cut(s) 622, 1021, 1336
Bse21I CCTNAGG 1 cut(s) 558
Bse3DI GCAATG 1 cut(s) 1368
BseBI CCWGG 2 cut(s) 181, 1216
BseDI CCNNGG 7 cut(s) 84, 179, 1004, 1215, 1240, 1531, 1618
BseGI GGATG 4 cut(s) 424, 490, 699, 861
BseLI CCNNNNNNNGG 4 cut(s) 411, 796, 1246, 1472
BseMI GCAATG 1 cut(s) 1368
BseMII CTCAG 2 cut(s) 572, 650
BseNI ACTGG 3 cut(s) 622, 1021, 1336
BseRI GAGGAG 3 cut(s) 578, 1037, 1220
BseSI GKGCMC 1 cut(s) 1455
BseXI GCAGC 7 cut(s) 26, 151, 169, 843, 1261, 1283, 1483
BsgI GTGCAG 1 cut(s) 150
Bsh1236I CGCG 1 cut(s) 1641
BshFI GGCC 7 cut(s) 35, 152, 404, 917, 1250, 1530, 1617
BshNI GGYRCC 1 cut(s) 7
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 2 cut(s) 215, 1128
BslFI GGGAC 1 cut(s) 1621
BslI CCNNNNNNNGG 4 cut(s) 411, 796, 1246, 1472
BsmAI GTCTC 1 cut(s) 1398
BsmFI GGGAC 1 cut(s) 1621
BsmI GAATGC 2 cut(s) 1052, 1378
BsnI GGCC 7 cut(s) 35, 152, 404, 917, 1250, 1530, 1617
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 1 cut(s) 1455
Bsp1407I TGTACA 1 cut(s) 1056
Bsp143I GATC 1 cut(s) 1505
BspACI CCGC 6 cut(s) 5, 131, 1110, 1593, 1605, 1641
BspANI GGCC 7 cut(s) 35, 152, 404, 917, 1250, 1530, 1617
BspCNI CTCAG 2 cut(s) 571, 651
BspFNI CGCG 1 cut(s) 1641
BspLI GGNNCC 3 cut(s) 9, 1086, 1616
BspMI ACCTGC 2 cut(s) 1352, 1495
BspPI GGATC 1 cut(s) 1513
BspQI GCTCTTC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 1368
BsrFI RCCGGY 1 cut(s) 214
BsrGI TGTACA 1 cut(s) 1056
BsrI ACTGG 3 cut(s) 622, 1021, 1336
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 7 cut(s) 84, 179, 1004, 1215, 1240, 1531, 1618
BssMI GATC 1 cut(s) 1505
BssT1I CCWWGG 2 cut(s) 84, 1240
Bst2UI CCWGG 2 cut(s) 181, 1216
Bst4CI ACNGT 1 cut(s) 1103
Bst6I CTCTTC 2 cut(s) 246, 1639
BstAPI GCANNNNNTGC 2 cut(s) 352, 929
BstAUI TGTACA 1 cut(s) 1056
BstBAI YACGTR 1 cut(s) 1201
BstC8I GCNNGC 5 cut(s) 68, 162, 216, 273, 357
BstDEI CTNAG 3 cut(s) 506, 558, 659
BstDSI CCRYGG 1 cut(s) 1531
BstF5I GGATG 4 cut(s) 424, 490, 699, 861
BstFNI CGCG 1 cut(s) 1641
BstKTI GATC 1 cut(s) 1508
BstMAI GTCTC 1 cut(s) 1398
BstMBI GATC 1 cut(s) 1505
BstMWI GCNNNNNNNGC 9 cut(s) 11, 14, 211, 352, 401, 907, 929, 1271, 1496
BstNI CCWGG 2 cut(s) 181, 1216
BstNSI RCATGY 1 cut(s) 1003
BstSCI CCNGG 2 cut(s) 179, 1214
BstSLI GKGCMC 1 cut(s) 1455
BstUI CGCG 1 cut(s) 1641
BstV1I GCAGC 7 cut(s) 26, 151, 169, 843, 1261, 1283, 1483
BstX2I RGATCY 1 cut(s) 1505
BstXI CCANNNNNNTGG 1 cut(s) 160
BstYI RGATCY 1 cut(s) 1505
Bsu36I CCTNAGG 1 cut(s) 558
BsuRI GGCC 7 cut(s) 35, 152, 404, 917, 1250, 1530, 1617
BtgI CCRYGG 1 cut(s) 1531
BtgZI GCGATG 1 cut(s) 1280
BtrI CACGTC 1 cut(s) 959
BtsCI GGATG 4 cut(s) 424, 490, 699, 861
BtsI GCAGTG 1 cut(s) 1373
BtsIMutI CAGTG 2 cut(s) 133, 1373
BveI ACCTGC 2 cut(s) 1352, 1495
Cac8I GCNNGC 5 cut(s) 68, 162, 216, 273, 357
CaiI CAGNNNCTG 1 cut(s) 17
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 4 cut(s) 403, 916, 1528, 1615
CseI GACGC 1 cut(s) 1647
Csp6I GTAC 1 cut(s) 1057
CviAII CATG 4 cut(s) 145, 1000, 1225, 1589
CviQI GTAC 1 cut(s) 1057
DdeI CTNAG 3 cut(s) 506, 558, 659
DpnI GATC 1 cut(s) 1507
DpnII GATC 1 cut(s) 1505
DraI TTTAAA 1 cut(s) 651
EaeI YGGCCR 1 cut(s) 1248
Eam1104I CTCTTC 2 cut(s) 246, 1639
EarI CTCTTC 2 cut(s) 246, 1639
EciI GGCGGA 1 cut(s) 1608
Eco130I CCWWGG 2 cut(s) 84, 1240
Eco32I GATATC 1 cut(s) 783
Eco57I CTGAAG 1 cut(s) 564
Eco81I CCTNAGG 1 cut(s) 558
Eco88I CYCGRG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 916
EcoRI GAATTC 1 cut(s) 1178
EcoRII CCWGG 2 cut(s) 179, 1214
EcoRV GATATC 1 cut(s) 783
EcoT14I CCWWGG 2 cut(s) 84, 1240
ErhI CCWWGG 2 cut(s) 84, 1240
FaeI CATG 4 cut(s) 148, 1003, 1228, 1592
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FaqI GGGAC 1 cut(s) 1621
FatI CATG 4 cut(s) 144, 999, 1224, 1588
FauI CCCGC 1 cut(s) 1598
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 9 cut(s) 6, 15, 132, 158, 165, 832, 1272, 1275, 1497
FokI GGATG 4 cut(s) 431, 497, 706, 848
Fsp4HI GCNGC 9 cut(s) 6, 15, 132, 158, 165, 832, 1272, 1275, 1497
FspBI CTAG 1 cut(s) 902
GluI GCNGC 9 cut(s) 6, 15, 132, 158, 165, 832, 1272, 1275, 1497
GsuI CTGGAG 3 cut(s) 639, 1038, 1560
HaeIII GGCC 7 cut(s) 35, 152, 404, 917, 1250, 1530, 1617
HapII CCGG 2 cut(s) 215, 1128
HgaI GACGC 1 cut(s) 1647
Hin1II CATG 4 cut(s) 148, 1003, 1228, 1592
HincII GTYRAC 1 cut(s) 1212
HindII GTYRAC 1 cut(s) 1212
HindIII AAGCTT 3 cut(s) 426, 508, 881
HinfI GANTC 4 cut(s) 72, 80, 953, 964
HpaI GTTAAC 1 cut(s) 1212
HpaII CCGG 2 cut(s) 215, 1128
HphI GGTGA 4 cut(s) 221, 931, 1058, 1163
Hpy166II GTNNAC 4 cut(s) 334, 1059, 1212, 1637
Hpy188III TCNNGA 5 cut(s) 46, 322, 494, 560, 887
Hpy8I GTNNAC 4 cut(s) 334, 1059, 1212, 1637
Hpy99I CGWCG 2 cut(s) 32, 214
HpyCH4III ACNGT 1 cut(s) 1103
HpyCH4IV ACGT 2 cut(s) 958, 1200
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 14, 211, 352, 401, 907, 929, 1271, 1496
HpyF3I CTNAG 3 cut(s) 506, 558, 659
HpySE526I ACGT 2 cut(s) 958, 1200
Hsp92II CATG 4 cut(s) 148, 1003, 1228, 1592
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
KspAI GTTAAC 1 cut(s) 1212
Kzo9I GATC 1 cut(s) 1505
LguI GCTCTTC 1 cut(s) 246
LmnI GCTCC 3 cut(s) 373, 620, 685
Lsp1109I GCAGC 7 cut(s) 26, 151, 169, 843, 1261, 1283, 1483
LweI GCATC 6 cut(s) 370, 672, 727, 748, 833, 1422
MaeI CTAG 1 cut(s) 902
MaeII ACGT 2 cut(s) 958, 1200
MaeIII GTNAC 5 cut(s) 613, 1112, 1123, 1196, 1655
MalI GATC 1 cut(s) 1507
MboI GATC 1 cut(s) 1505
MflI RGATCY 1 cut(s) 1505
MhlI GDGCHC 1 cut(s) 1455
MluCI AATT 5 cut(s) 711, 743, 836, 1178, 1232
MlyI GAGTC 2 cut(s) 66, 89
MmeI TCCRAC 1 cut(s) 51
MreI CGCCGGCG 1 cut(s) 214
MroNI GCCGGC 1 cut(s) 214
MseI TTAA 6 cut(s) 537, 650, 989, 1211, 1235, 1563
MslI CAYNNNNRTG 1 cut(s) 341
MspA1I CMGCKG 4 cut(s) 17, 160, 985, 1274
MspI CCGG 2 cut(s) 215, 1128
MspR9I CCNGG 2 cut(s) 181, 1216
Mva1269I GAATGC 2 cut(s) 1052, 1378
MvaI CCWGG 2 cut(s) 181, 1216
MvnI CGCG 1 cut(s) 1641
MwoI GCNNNNNNNGC 9 cut(s) 11, 14, 211, 352, 401, 907, 929, 1271, 1496
NaeI GCCGGC 1 cut(s) 216
NdeII GATC 1 cut(s) 1505
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 4 cut(s) 148, 1003, 1228, 1592
NlaIV GGNNCC 3 cut(s) 9, 1086, 1616
NmeAIII GCCGAG 1 cut(s) 1643
NspI RCATGY 1 cut(s) 1003
PciSI GCTCTTC 1 cut(s) 246
PcsI WCGNNNNNNNCGW 3 cut(s) 63, 111, 215
PctI GAATGC 2 cut(s) 1052, 1378
PdiI GCCGGC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 953, 964
PflMI CCANNNNNTGG 1 cut(s) 1246
PkrI GCNGC 9 cut(s) 7, 16, 133, 159, 166, 833, 1273, 1276, 1498
PleI GAGTC 2 cut(s) 66, 88
PpsI GAGTC 2 cut(s) 66, 88
Ppu21I YACGTR 1 cut(s) 1201
Psp6I CCWGG 2 cut(s) 179, 1214
PspGI CCWGG 2 cut(s) 179, 1214
PspN4I GGNNCC 3 cut(s) 9, 1086, 1616
PspPI GGNCC 4 cut(s) 403, 916, 1528, 1615
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 1505
PvuII CAGCTG 4 cut(s) 17, 160, 985, 1274
RsaI GTAC 1 cut(s) 1058
RsaNI GTAC 1 cut(s) 1057
RseI CAYNNNNRTG 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 6 cut(s) 537, 650, 989, 1211, 1235, 1563
SatI GCNGC 9 cut(s) 6, 15, 132, 158, 165, 832, 1272, 1275, 1497
Sau3AI GATC 1 cut(s) 1505
Sau96I GGNCC 4 cut(s) 403, 916, 1528, 1615
SchI GAGTC 2 cut(s) 66, 89
ScrFI CCNGG 2 cut(s) 181, 1216
SduI GDGCHC 1 cut(s) 1455
SfaNI GCATC 6 cut(s) 370, 672, 727, 748, 833, 1422
SgrAI CRCCGGYG 1 cut(s) 214
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 2 cut(s) 911, 1456
SmoI CTYRAG 2 cut(s) 911, 1456
Sse9I AATT 5 cut(s) 711, 743, 836, 1178, 1232
SsiI CCGC 6 cut(s) 5, 131, 1110, 1593, 1605, 1641
SspMI CTAG 1 cut(s) 902
StyD4I CCNGG 2 cut(s) 179, 1214
StyI CCWWGG 2 cut(s) 84, 1240
TaaI ACNGT 1 cut(s) 1103
TaiI ACGT 2 cut(s) 961, 1203
TaqI TCGA 6 cut(s) 45, 105, 265, 323, 771, 1044
TasI AATT 5 cut(s) 711, 743, 836, 1178, 1232
TatI WGTACW 1 cut(s) 1056
TauI GCSGC 2 cut(s) 8, 134
TfiI GAWTC 2 cut(s) 953, 964
Tru1I TTAA 6 cut(s) 537, 650, 989, 1211, 1235, 1563
Tru9I TTAA 6 cut(s) 537, 650, 989, 1211, 1235, 1563
TscAI CASTG 2 cut(s) 133, 1380
TseI GCWGC 7 cut(s) 14, 157, 164, 831, 1271, 1274, 1496
TspDTI ATGAA 6 cut(s) 161, 483, 906, 1241, 1302, 1383
TspGWI ACGGA 1 cut(s) 706
TspRI CASTG 2 cut(s) 133, 1380
Van91I CCANNNNNTGG 1 cut(s) 1246
XapI RAATTY 2 cut(s) 836, 1178
XceI RCATGY 1 cut(s) 1003
XmiI GTMKAC 1 cut(s) 333
XspI CTAG 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.