MD05G1306200.v1.1

Apoptosis inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0004487
Physical Location & Seq
Reverse (-)
43833824 .. 43840495
6672 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1306200.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGGCCGACCCCTCCGACGAGGCTAAGCATATCGAGAAGCTTTACGAGTTTGGCGAACGCCTCAACGAGTCCAAGGACAAGTCCCAGAATGTCAAGGACTACCAGGGGATTATCAATGCGGCCAAGACGAGTGTCAAGGCCAAGCAGCTGGCTGCCCAGCTTATTCCGAGGTTCTTCAAGTTCTTCCCCGAACTTTCGCAGCCCGCCATCGATACCCACCTCGATTTGATTGAAGAAGAAGAGCTCGGGGTACGAGTACAAGCTATTCGGGGCCTTCCCCTTTTTTGCAAGGATACGCCTGAACACATTGCGAAAATTGTAGACATTCTTGTGCAACTCCTTGCATCTGAGGAATTTGTGGAGCGTGATGCTGTGCATAAAGCACTTATGGCCCTTTTGAGGCAAGATGTTAAAGCTTCTTTGACAGCATTATTCAAGCACATTGGGAGTGTTGATGAACCAAGCACAGATGAGTTCATTCGCGAGAAGGTTTTAAGCTTCATTAGAGATAAGGTATTTCCTATTAAAGCTGAACTTTTGAAGCCTCAGGAGGAAATGGAAAGGCATATTACTGATTTAATAAAAAAGAGCTTGGAAGATGTAACTGGTGCAGAGTTTAGAATGTTTATGGACTTCTTAAAAAGTTTGAGCATATTTGGGGAGAAAGCTCCTGCCGAACGCATGAAAGAACTTATTGGAATTATTGAAGGACAAGCTGATTTGGATGCCCAATTCAATGTTTCAGATGCTGATCATATTGACAGGTTGATATCCTGCCTATTCATGGCTCTACCATTTGTTGTGAGGGGTGCATCAAGCAGCAAGTTCCTAAACTATCTGGACAAACACATCCTTCCTGTTTCGGATAAGCTTCCTGATGAAAGAAGGCTAGATTTGCTAAAAGCCCTTGCAGAAGTTTCACCTTTCACAGCACCACAAGATTCTCGTCAGATTCTTCCTTCTGTTGTTCAGCTGTTGAAGAAAAACATGCCTCGGAGAAAGACTGGAGAGGAGTTTAACTTCACTTACGTAGAATGCTTGTTGTACACTTTTCACCATCTTGCTCACAAGGTTCCTAATGCTACTAACAGTCTATGCGGTTACAAGATAGTGACTGGGCAACCATCAGATAGGCTTGGCGAGGACTTTTCGGAGCAATATAAGGAGTTTACTGAGAGGTTGAGCTATGTTGAGGAGCTAACTAGGGCCACCATGAAGAAGTTAACACAGGGAATGGCTGAAAAGAACAAAGCGATGGCAGCTGCAAAGTCTGAAGAAGCAAAGAATACCATTAAAACTGAAAAGCAGAATACTACAACTGGGTTGCGAACCTGCAATAACATATTGACAATGACAAAGACTCTGCATTCAAAAACACCCTCGTTCATTGGAGATAAGAGCATCAATCTGTCTTGGAAAGAAGCAACAAAACCTGCTGTGCCCTCTAGCTCGCCGACCACAGGAGCAAAACGACCTGCTAATTTTTCCAATGGATCTGGAAATATGCCTTCAAAAAAGAGCCGTGGAGGTGGCGGTTTACAAAACCAGCTTGTTAATAGAGCATTTGAAGGTCTATCTCAAGGTGGAAGAAGCGGGGGAAGGAGCGGTGGAGGAAGGAGCAGAGGCAGAGGGTGGGGTGGACGTGGAAGGGGAAGGGGTTACAGATAG

Protein Analysis

556

Amino Acids

61.95

Weight (kDa)

9.08

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
API5 PF05918 11 - 553 1.4e-247 Apoptosis inhibitory protein 5 (API5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1340, 1441, 1483
AccBSI CCGCTC 1 cut(s) 1605
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 483
AciI CCGC 6 cut(s) 119, 204, 1098, 1533, 1593, 1605
AclWI GGATC 1 cut(s) 1501
AcoI YGGCCR 2 cut(s) 3, 120
AcsI RAATTY 1 cut(s) 353
AcuI CTGAAG 1 cut(s) 1293
AfaI GTAC 3 cut(s) 252, 258, 1046
AfiI CCNNNNNNNGG 3 cut(s) 399, 784, 1460
AjiI CACGTC 1 cut(s) 1643
AjnI CCWGG 1 cut(s) 102
Alw21I GWGCWC 1 cut(s) 246
AlwI GGATC 1 cut(s) 1501
AlwNI CAGNNNCTG 1 cut(s) 749
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 6 cut(s) 3, 120, 138, 271, 390, 1206
ApeKI GCWGC 6 cut(s) 145, 152, 199, 819, 1259, 1262
ApoI RAATTY 1 cut(s) 353
AspS9I GGNCC 3 cut(s) 271, 391, 1206
AsuHPI GGTGA 2 cut(s) 912, 1046
AvaI CYCGRG 1 cut(s) 245
AxyI CCTNAGG 1 cut(s) 546
BaeGI GKGCMC 1 cut(s) 1443
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 6 cut(s) 139, 157, 211, 831, 1249, 1271
BccI CCATC 4 cut(s) 215, 1065, 1132, 1249
BceAI ACGGC 1 cut(s) 1506
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 1 cut(s) 286
BclI TGATCA 1 cut(s) 751
BfaI CTAG 3 cut(s) 890, 1203, 1446
BfuAI ACCTGC 3 cut(s) 1340, 1441, 1483
BfuI GTATCC 1 cut(s) 286
BisI GCNGC 7 cut(s) 120, 146, 153, 200, 820, 1260, 1263
BlpI GCTNAGC 1 cut(s) 24
BlsI GCNGC 7 cut(s) 121, 147, 154, 201, 821, 1261, 1264
Bme1390I CCNGG 1 cut(s) 104
BmeT110I CYCGRG 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 1643
BmgT120I GGNCC 3 cut(s) 271, 391, 1206
BmiI GGNNCC 2 cut(s) 272, 1074
BmrFI CCNGG 1 cut(s) 104
BmrI ACTGGG 2 cut(s) 1125, 1329
BmsI GCATC 6 cut(s) 353, 358, 715, 736, 821, 1410
BmuI ACTGGG 2 cut(s) 1125, 1329
BoxI GACNNNNGTC 1 cut(s) 131
BpmI CTGGAG 1 cut(s) 1026
Bpu1102I GCTNAGC 1 cut(s) 24
BpuEI CTTGAG 1 cut(s) 1563
Bsa29I ATCGAT 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 1030
BsaBI GATNNNNATC 1 cut(s) 750
BsaJI CCNNGG 5 cut(s) 72, 103, 167, 992, 1522
Bsc4I CCNNNNNNNGG 3 cut(s) 399, 784, 1460
Bse1I ACTGG 4 cut(s) 610, 1009, 1120, 1324
Bse21I CCTNAGG 1 cut(s) 546
Bse3DI GCAATG 1 cut(s) 306
Bse8I GATNNNNATC 1 cut(s) 750
BseBI CCWGG 1 cut(s) 104
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 5 cut(s) 72, 103, 167, 992, 1522
BseGI GGATG 2 cut(s) 730, 849
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 3 cut(s) 399, 784, 1460
BseMI GCAATG 1 cut(s) 306
BseMII CTCAG 3 cut(s) 339, 560, 1164
BseNI ACTGG 4 cut(s) 610, 1009, 1120, 1324
BseRI GAGGAG 2 cut(s) 1025, 1208
BseSI GKGCMC 1 cut(s) 1443
BseXI GCAGC 6 cut(s) 139, 157, 211, 831, 1249, 1271
BseYI CCCAGC 1 cut(s) 156
BsgI GTGCAG 1 cut(s) 630
Bsh1236I CGCG 1 cut(s) 483
BshFI GGCC 6 cut(s) 5, 122, 140, 273, 392, 1208
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 245
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 3 cut(s) 399, 784, 1460
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 2 cut(s) 1040, 1366
BsnI GGCC 6 cut(s) 5, 122, 140, 273, 392, 1208
BsoBI CYCGRG 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 246, 1443
Bsp1407I TGTACA 1 cut(s) 1044
Bsp143I GATC 2 cut(s) 751, 1493
Bsp1720I GCTNAGC 1 cut(s) 24
Bsp68I TCGCGA 1 cut(s) 483
BspACI CCGC 6 cut(s) 119, 204, 1098, 1533, 1593, 1605
BspANI GGCC 6 cut(s) 5, 122, 140, 273, 392, 1208
BspCNI CTCAG 3 cut(s) 340, 559, 1165
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 1 cut(s) 483
BspLI GGNNCC 2 cut(s) 272, 1074
BspMI ACCTGC 3 cut(s) 1340, 1441, 1483
BspPI GGATC 1 cut(s) 1501
BspQI GCTCTTC 1 cut(s) 234
BsrBI CCGCTC 1 cut(s) 1605
BsrDI GCAATG 1 cut(s) 306
BsrGI TGTACA 1 cut(s) 1044
BsrI ACTGG 4 cut(s) 610, 1009, 1120, 1324
BssECI CCNNGG 5 cut(s) 72, 103, 167, 992, 1522
BssMI GATC 2 cut(s) 751, 1493
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 1091
Bst6I CTCTTC 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 1044
BstBAI YACGTR 1 cut(s) 1030
BstC8I GCNNGC 3 cut(s) 150, 204, 1451
BstDEI CTNAG 4 cut(s) 24, 348, 546, 1173
BstDSI CCRYGG 1 cut(s) 1522
BstF5I GGATG 2 cut(s) 730, 849
BstFNI CGCG 1 cut(s) 483
BstKTI GATC 2 cut(s) 754, 1496
BstMBI GATC 2 cut(s) 751, 1493
BstMWI GCNNNNNNNGC 3 cut(s) 389, 895, 1259
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 991
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 102
BstSLI GKGCMC 1 cut(s) 1443
BstSNI TACGTA 1 cut(s) 1030
BstUI CGCG 1 cut(s) 483
BstV1I GCAGC 6 cut(s) 139, 157, 211, 831, 1249, 1271
BstX2I RGATCY 1 cut(s) 1493
BstXI CCANNNNNNTGG 1 cut(s) 148
BstYI RGATCY 1 cut(s) 1493
Bsu15I ATCGAT 1 cut(s) 210
Bsu36I CCTNAGG 1 cut(s) 546
BsuI GTATCC 1 cut(s) 286
BsuRI GGCC 6 cut(s) 5, 122, 140, 273, 392, 1208
BsuTUI ATCGAT 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 1522
BtgZI GCGATG 1 cut(s) 1268
BtrI CACGTC 1 cut(s) 1643
BtsCI GGATG 2 cut(s) 730, 849
BtuMI TCGCGA 1 cut(s) 483
BveI ACCTGC 3 cut(s) 1340, 1441, 1483
Cac8I GCNNGC 3 cut(s) 150, 204, 1451
CaiI CAGNNNCTG 1 cut(s) 749
Cfr13I GGNCC 3 cut(s) 271, 391, 1206
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 3 cut(s) 251, 257, 1045
CviAII CATG 4 cut(s) 682, 784, 988, 1213
CviQI GTAC 3 cut(s) 251, 257, 1045
DdeI CTNAG 4 cut(s) 24, 348, 546, 1173
DpnI GATC 2 cut(s) 753, 1495
DpnII GATC 2 cut(s) 751, 1493
EaeI YGGCCR 2 cut(s) 3, 120
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 244
Eco105I TACGTA 1 cut(s) 1030
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 246
Eco32I GATATC 1 cut(s) 771
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 1293
Eco81I CCTNAGG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 102
EcoRV GATATC 1 cut(s) 771
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 4 cut(s) 685, 787, 991, 1216
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FaqI GGGAC 1 cut(s) 67
FatI CATG 4 cut(s) 681, 783, 987, 1212
FauI CCCGC 2 cut(s) 211, 1586
FbaI TGATCA 1 cut(s) 751
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 7 cut(s) 120, 146, 153, 200, 820, 1260, 1263
FokI GGATG 2 cut(s) 737, 836
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 7 cut(s) 120, 146, 153, 200, 820, 1260, 1263
FspBI CTAG 3 cut(s) 890, 1203, 1446
GluI GCNGC 7 cut(s) 120, 146, 153, 200, 820, 1260, 1263
GsaI CCCAGC 1 cut(s) 160
GsuI CTGGAG 1 cut(s) 1026
HaeIII GGCC 6 cut(s) 5, 122, 140, 273, 392, 1208
Hin1II CATG 4 cut(s) 685, 787, 991, 1216
HincII GTYRAC 1 cut(s) 1224
HindII GTYRAC 1 cut(s) 1224
HindIII AAGCTT 4 cut(s) 38, 414, 496, 869
HinfI GANTC 4 cut(s) 68, 941, 952, 1360
HpaI GTTAAC 1 cut(s) 1224
HphI GGTGA 2 cut(s) 912, 1046
Hpy166II GTNNAC 6 cut(s) 322, 1047, 1170, 1224, 1538, 1640
Hpy188III TCNNGA 6 cut(s) 34, 482, 548, 839, 875, 1497
Hpy8I GTNNAC 6 cut(s) 322, 1047, 1170, 1224, 1538, 1640
Hpy99I CGWCG 1 cut(s) 20
HpyCH4III ACNGT 1 cut(s) 1091
HpyCH4IV ACGT 2 cut(s) 1029, 1642
HpyF10VI GCNNNNNNNGC 3 cut(s) 389, 895, 1259
HpyF3I CTNAG 4 cut(s) 24, 348, 546, 1173
HpySE526I ACGT 2 cut(s) 1029, 1642
Hsp92II CATG 4 cut(s) 685, 787, 991, 1216
Ksp22I TGATCA 1 cut(s) 751
KspAI GTTAAC 1 cut(s) 1224
Kzo9I GATC 2 cut(s) 751, 1493
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 7 cut(s) 361, 673, 1153, 1195, 1463, 1602, 1617
Lsp1109I GCAGC 6 cut(s) 139, 157, 211, 831, 1249, 1271
LweI GCATC 6 cut(s) 353, 358, 715, 736, 821, 1410
MaeI CTAG 3 cut(s) 890, 1203, 1446
MaeII ACGT 2 cut(s) 1029, 1642
MaeIII GTNAC 4 cut(s) 601, 1100, 1111, 1658
MalI GATC 2 cut(s) 753, 1495
MbiI CCGCTC 1 cut(s) 1605
MboI GATC 2 cut(s) 751, 1493
MflI RGATCY 1 cut(s) 1493
MhlI GDGCHC 2 cut(s) 246, 1443
MluCI AATT 5 cut(s) 315, 353, 699, 731, 1480
MlyI GAGTC 2 cut(s) 77, 1354
MmeI TCCRAC 1 cut(s) 39
MseI TTAA 9 cut(s) 411, 494, 525, 578, 638, 1017, 1223, 1293, 1554
MslI CAYNNNNRTG 1 cut(s) 329
MspA1I CMGCKG 3 cut(s) 148, 973, 1262
MspR9I CCNGG 1 cut(s) 104
Mva1269I GAATGC 2 cut(s) 1040, 1366
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 1 cut(s) 483
MwoI GCNNNNNNNGC 3 cut(s) 389, 895, 1259
NdeII GATC 2 cut(s) 751, 1493
NlaIII CATG 4 cut(s) 685, 787, 991, 1216
NlaIV GGNNCC 2 cut(s) 272, 1074
NmuCI GTSAC 1 cut(s) 1111
NruI TCGCGA 1 cut(s) 483
NspI RCATGY 1 cut(s) 991
PciSI GCTCTTC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 2 cut(s) 1040, 1366
PfeI GAWTC 2 cut(s) 941, 952
PkrI GCNGC 7 cut(s) 121, 147, 154, 201, 821, 1261, 1264
PleI GAGTC 2 cut(s) 76, 1354
PpsI GAGTC 2 cut(s) 76, 1354
Ppu21I YACGTR 1 cut(s) 1030
PshAI GACNNNNGTC 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 102
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 2 cut(s) 272, 1074
PspPI GGNCC 3 cut(s) 271, 391, 1206
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 1 cut(s) 1493
PvuII CAGCTG 3 cut(s) 148, 973, 1262
RruI TCGCGA 1 cut(s) 483
RsaI GTAC 3 cut(s) 252, 258, 1046
RsaNI GTAC 3 cut(s) 251, 257, 1045
RseI CAYNNNNRTG 1 cut(s) 329
SacI GAGCTC 1 cut(s) 246
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 9 cut(s) 411, 494, 525, 578, 638, 1017, 1223, 1293, 1554
SatI GCNGC 7 cut(s) 120, 146, 153, 200, 820, 1260, 1263
Sau3AI GATC 2 cut(s) 751, 1493
Sau96I GGNCC 3 cut(s) 271, 391, 1206
SchI GAGTC 2 cut(s) 77, 1354
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 2 cut(s) 246, 1443
SfaNI GCATC 6 cut(s) 353, 358, 715, 736, 821, 1410
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 1578
SmoI CTYRAG 1 cut(s) 1578
SnaBI TACGTA 1 cut(s) 1030
Sse9I AATT 5 cut(s) 315, 353, 699, 731, 1480
SsiI CCGC 6 cut(s) 119, 204, 1098, 1533, 1593, 1605
SspMI CTAG 3 cut(s) 890, 1203, 1446
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 1091
TaiI ACGT 2 cut(s) 1032, 1645
TaqI TCGA 3 cut(s) 33, 210, 222
TasI AATT 5 cut(s) 315, 353, 699, 731, 1480
TatI WGTACW 2 cut(s) 256, 1044
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 2 cut(s) 941, 952
Tru1I TTAA 9 cut(s) 411, 494, 525, 578, 638, 1017, 1223, 1293, 1554
Tru9I TTAA 9 cut(s) 411, 494, 525, 578, 638, 1017, 1223, 1293, 1554
TseFI GTSAC 1 cut(s) 1111
TseI GCWGC 6 cut(s) 145, 152, 199, 819, 1259, 1262
Tsp45I GTSAC 1 cut(s) 1111
TspDTI ATGAA 8 cut(s) 466, 471, 490, 698, 772, 894, 1229, 1375
XapI RAATTY 1 cut(s) 353
XceI RCATGY 1 cut(s) 991
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 3 cut(s) 890, 1203, 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.