Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0004487

Physical Location & Seq

Genomic Coordinates Forward (+)

60247519 .. 60249738

Gene Structure 2220 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0446700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2220 bp

ATGCCTCAGAAAAACACTTTCTCAGTAAACCTGATGATTTCCAGTTATGTCAACTGTGGTAATCTTTATAAGGCCATGGTTGAGCGTACAGCTGTAACATGGATAGGTGGGTATTCGCAAGCTGGTCAATACCGTGAAGCATTTAAACTTTACGCCGAGATGCATCGGTGGGGAACTAAGCCGGATTATGTGACTTTTGCAACTCTGTTATCAGGATGCAGCAACATGGATGCTACAAAGGAAGTAGTTCAGGTTCATTCTCATATCCTTAAATTGGGATATCATTCTACACTCATGGTCTGCAATTCTTTGGTTGATTCCTACTGCAAATCACATTGCCTGGATTTTGCTTTTCTGCTTTTCAAGGAAATGCCTGAAAGAGATGCTGTTACATTCAATGCATTGATAACAGGGTCCTCGAAACATGGGTTGAATGAAGGTGCTATTAACCTCTTTGCTGAGATGCAAAATCTGGGTTACAAGCCTTCTGAATTTACATTTGCAGCAGTACTTTGTGCCGCTATCGGTTTAGATGACACTGTGCTTGGGCAACAGGTTCATGGTTTTGTGGCTAAGACTAATTTTGTTTGGAACGTTTTTGTGGGAAATGCATTGCTCGATTTCTACTCAAAGCACGAATATGTGGTTGAAGCAAGGAAACTTTTTTATGAGATGCCAGAGTTGGATTGTATTTCTTACAATATAATCATCTCCTCCTATGTATGGGATGGGCAATTTAAAGAGGCTCTTGATCTTTTCCGTGAATTGCAGTTTTCCAAATATGACCGGAAGCAATTCCCTTTTGCAACCATGTTGAGCATAGCAGCGAATACTCTGAACATAAATGTGGGCCGGCAGATACATTCCCAGGCCATTGTAGCAACAGCTGATTCAGAATCTCAGGTTGGAAATTCTTTGGTTGACATGTATGGCAAATGTGGTAGATTTACCGAAGCCAGAAGAATATTTGCTAGTCTGGCTGACAAAAGTACAGTGCCATGGACTGCACTCATCTCAGCATATGTTCAGAAGGGATTCCATGAAGAAGGCCTGGAACTGTACAATGAGATGTGCAGAGGAAATGTTAGTCCCGACCAGGCCACTTTCGCAAGCATCTTAAGAGCCTCTTCAAATTTGGCTTCACTCTCACTAGGAAAGCAGTTACACTCATCAGTCATCATATTAGGATTCATTTGCAACGTTTTTCCTGCAAGTGCGCTTCTAGATATGTATGCGAAATGTGGATCCATGAAAGATGCTCTTCAGATTTTTCAGGAGATGCCAAAGAGAAATATTGTATGTTGGAATGCCCTGATCTCAGCTTATGCACAAAATGGTGATGGTAAAGGGACTCTTAGATCGTTTGAATGGATGGTTCAGTCAGGTTTTGCACCAGATTCTGTTAGCTTCTTGAGCATTTTAACTGCTTGTAGCCACTGTGGGCTTGTTGAACAAGGACTATATTACTTCAACTCCTTGATGCATAACTCTAACATTGTTCCAAAGAGAGAACACTATGCATCAATGGTGGATGTGCTGTGTCGAAGTGGGAGATTCGATGAAGGAGAGAAATTAATGGTTCGAATGCCATTTGAGCCTGATGAGATTATGTGGTCGTCTGTACTAAACTCATGCAGAATTCATAAGAATCAAGAATTGGCAGAGAGAGCCGCAAACAGGCTTTTCAATATGGTCGAGCTTAGGGATGCAGCTGCTTATGTCAACATGTCAAACATTTATGCAGAAATAGGTGAGTGGGAGAGTTTAAGGGAGGTGAAGAAGGCGATGAGGGAGAGAGGAGTGAGAAAGGTTACAGCGTATAGTTGGGTAGAAATCAAACACAAGATTCATGTATTTACAGTGAATGATAATAGCCATCCAGAGAGTGGAAAAATTATGTCAAAAATTGATGAGTTGGTGAAGGAGATGGAAAAGGAAGGATACAAGGCTGACACAAGTTGTGCCCTTCACAATGAAGATGAGGAAATAAAAGTGGAGTCTCTCAAATATCACAGTGAACGCTTAGCGATTGCATTTGCATTGATCAGCACACCAGAAGGTTCGCCTATAGTGGTGATGAAGAATTTACGAGCTTGCACAGACTGCCATGCTGCAATCAAAGTGATCACGAAGATTGTTGGGAGGGAAATAACAGTTAGAGACTCGAGCAGGTTCCATCATTTTAGAGATGGGCTTTGCTCCTGTGGGGACTTTTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0009555 GO:0010941 GO:0032501 GO:0032502 GO:0043067 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048229 GO:0048856 GO:0050789 GO:0050794 GO:0065007

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

API5

Protein Analysis

739

Amino Acids

83.24

Weight (kDa)

6.38

Isoelectric Point (pI)

41.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	35 - 75	2.8e-11	PPR repeat family
PPR_2	PF13041	128 - 171	1.9e-09	PPR repeat family
PPR_2	PF13041	330 - 375	1.4e-09	PPR repeat family
PPR	PF01535	333 - 360	1.3e-06	PPR repeat
PPR_2	PF13041	430 - 478	2e-10	PPR repeat family
E_motif	PF20431	548 - 611	1.3e-12	E motif
DYW_deaminase	PF14432	647 - 739	2.5e-37	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004487)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34040 AT2G34040 AT2G34040
fragaria_vesca	FvH4_3g08240 FvH4_3g08240 FvH4_3g08240
malus_domestica	MD05G1306200.v1.1 MD10G1285200.v1.1
prunus_persica	Prupe.4G056400_v2.0.a1 Prupe.4G056400_v2.0.a1 Prupe.4G056400_v2.0.a1
pyrus_communis	pycom05g28330 pycom10g23830
rosa_chinensis	RchiOBHm_Chr5g0009911 RchiOBHm_Chr7g0217661
rosa_laevigata	RLG00000002451 RLG00000031709
rosa_multiflora	Rmu_sc0001300.1_g000008 Rmu_sc0001709.1_g000015
rosa_roxburghii	Rroxscaffold_1G00066270
rosa_rugosa	Rorug04G0446700 Rorug04G0446800 Rorug07G0170700
rosa_samantha	Rh5AG078400 Rh5BG073300 Rh5CG085700 Rh5DG073800 Rh7AG310900 Rh7BG302600 Rh7CG329800
rosa_wichuraiana	Rw5G007210 Rw7G026380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	69
Acc36I	ACCTGC	1 cut(s)	2160
AccB7I	CCANNNNNTGG	1 cut(s)	1886
AciI	CCGC	2 cut(s)	519, 1671
AclI	AACGTT	2 cut(s)	594, 1200
AclWI	GGATC	2 cut(s)	1239, 1252
AcsI	RAATTY	5 cut(s)	491, 910, 1132, 1638, 2083
AcuI	CTGAAG	1 cut(s)	1247
AfaI	GTAC	5 cut(s)	88, 510, 991, 1061, 1623
AfiI	CCNNNNNNNGG	4 cut(s)	274, 723, 1677, 1886
AflII	CTTAAG	1 cut(s)	1117
AflIII	ACRYGT	2 cut(s)	924, 1725
AgsI	TTSAA	9 cut(s)	364, 397, 433, 650, 1131, 1367, 1451, 1471, 1687
AjnI	CCWGG	4 cut(s)	339, 867, 1050, 1095
AjuI	GAANNNNNNNTTGG	4 cut(s)	888, 920, 1640, 1672
AluBI	AGCT	8 cut(s)	92, 122, 887, 1322, 1407, 1699, 1712, 2093
AluI	AGCT	8 cut(s)	92, 122, 887, 1322, 1407, 1699, 1712, 2093
Alw26I	GTCTC	2 cut(s)	2004, 2154
AlwI	GGATC	2 cut(s)	1239, 1252
AlwNI	CAGNNNCTG	1 cut(s)	1400
Ama87I	CYCGRG	1 cut(s)	2164
AoxI	GGCC	5 cut(s)	72, 850, 870, 1048, 1098
ApeKI	GCWGC	6 cut(s)	219, 503, 824, 1709, 1712, 2111
ApoI	RAATTY	5 cut(s)	491, 910, 1132, 1638, 2083
AseI	ATTAAT	1 cut(s)	1574
Asp700I	GAANNNNTTC	3 cut(s)	246, 794, 1034
AspLEI	GCGC	1 cut(s)	1219
AspS9I	GGNCC	2 cut(s)	414, 850
AsuHPI	GGTGA	5 cut(s)	1349, 1763, 1786, 1930, 2086
AsuII	TTCGAA	1 cut(s)	1582
AvaI	CYCGRG	1 cut(s)	2164
AvaII	GGWCC	1 cut(s)	414
BaeGI	GKGCMC	1 cut(s)	1966
BamHI	GGATCC	1 cut(s)	1244
BbvI	GCAGC	6 cut(s)	231, 515, 836, 1699, 1721, 2098
BccI	CCATC	7 cut(s)	722, 1334, 1366, 1884, 1921, 2183, 2184
BciT130I	CCWGG	4 cut(s)	341, 869, 1052, 1097
BciVI	GTATCC	1 cut(s)	1934
BclI	TGATCA	2 cut(s)	2043, 2124
BcoDI	GTCTC	2 cut(s)	2004, 2154
BfaI	CTAG	3 cut(s)	972, 1151, 1223
BfmI	CTRYAG	1 cut(s)	2067
BfrI	CTTAAG	1 cut(s)	1117
BfuAI	ACCTGC	1 cut(s)	2160
BfuI	GTATCC	1 cut(s)	1934
BisI	GCNGC	8 cut(s)	220, 504, 519, 825, 1671, 1710, 1713, 2112
BlpI	GCTNAGC	1 cut(s)	2023
BlsI	GCNGC	8 cut(s)	221, 505, 520, 826, 1672, 1711, 1714, 2113
BmcAI	AGTACT	1 cut(s)	510
Bme1390I	CCNGG	4 cut(s)	341, 869, 1052, 1097
Bme18I	GGWCC	1 cut(s)	414
BmeT110I	CYCGRG	1 cut(s)	2164
BmgT120I	GGNCC	2 cut(s)	414, 850
BmiI	GGNNCC	3 cut(s)	415, 1246, 2174
BmrFI	CCNGG	4 cut(s)	341, 869, 1052, 1097
Bpu10I	CCTNAGC	1 cut(s)	1700
Bpu1102I	GCTNAGC	1 cut(s)	2023
Bpu14I	TTCGAA	1 cut(s)	1582
BpuEI	CTTGAG	1 cut(s)	1432
BsaJI	CCNNGG	3 cut(s)	75, 867, 998
BsaWI	WCCGGW	1 cut(s)	786
BsaXI	ACNNNNNCTCC	2 cut(s)	1457, 1487
Bsc4I	CCNNNNNNNGG	4 cut(s)	274, 723, 1677, 1886
Bse118I	RCCGGY	1 cut(s)	852
Bse1I	ACTGG	1 cut(s)	42
Bse3DI	GCAATG	2 cut(s)	334, 611
BseBI	CCWGG	4 cut(s)	341, 869, 1052, 1097
BseDI	CCNNGG	3 cut(s)	75, 867, 998
BseGI	GGATG	7 cut(s)	221, 235, 733, 1377, 1537, 1711, 1876
BseLI	CCNNNNNNNGG	4 cut(s)	274, 723, 1677, 1886
BseMI	GCAATG	2 cut(s)	334, 611
BseMII	CTCAG	6 cut(s)	20, 36, 450, 914, 1029, 1332
BseNI	ACTGG	1 cut(s)	42
BseRI	GAGGAG	2 cut(s)	703, 1812
BseSI	GKGCMC	1 cut(s)	1966
BseXI	GCAGC	6 cut(s)	231, 515, 836, 1699, 1721, 2098
BsgI	GTGCAG	2 cut(s)	990, 1093
BshFI	GGCC	5 cut(s)	74, 852, 872, 1050, 1100
BsiHKCI	CYCGRG	1 cut(s)	2164
BsiSI	CCGG	3 cut(s)	182, 787, 853
BslFI	GGGAC	2 cut(s)	1074, 1363
BslI	CCNNNNNNNGG	4 cut(s)	274, 723, 1677, 1886
BsmAI	GTCTC	2 cut(s)	2004, 2154
BsmFI	GGGAC	2 cut(s)	1074, 1363
BsmI	GAATGC	2 cut(s)	1312, 1590
BsnI	GGCC	5 cut(s)	74, 852, 872, 1050, 1100
BsoBI	CYCGRG	1 cut(s)	2164
Bsp119I	TTCGAA	1 cut(s)	1582
Bsp1286I	GDGCHC	1 cut(s)	1966
Bsp1407I	TGTACA	1 cut(s)	1059
Bsp143I	GATC	6 cut(s)	751, 1244, 1314, 1358, 2043, 2124
Bsp1720I	GCTNAGC	1 cut(s)	2023
Bsp19I	CCATGG	2 cut(s)	75, 998
BspACI	CCGC	2 cut(s)	519, 1671
BspANI	GGCC	5 cut(s)	74, 852, 872, 1050, 1100
BspCNI	CTCAG	6 cut(s)	19, 35, 451, 913, 1028, 1331
BspLI	GGNNCC	3 cut(s)	415, 1246, 2174
BspMI	ACCTGC	1 cut(s)	2160
BspPI	GGATC	2 cut(s)	1239, 1252
BspQI	GCTCTTC	1 cut(s)	1266
BspT104I	TTCGAA	1 cut(s)	1582
BspTI	CTTAAG	1 cut(s)	1117
BsrDI	GCAATG	2 cut(s)	334, 611
BsrFI	RCCGGY	1 cut(s)	852
BsrGI	TGTACA	1 cut(s)	1059
BsrI	ACTGG	1 cut(s)	42
BssAI	RCCGGY	1 cut(s)	852
BssECI	CCNNGG	3 cut(s)	75, 867, 998
BssMI	GATC	6 cut(s)	751, 1244, 1314, 1358, 2043, 2124
BssT1I	CCWWGG	2 cut(s)	75, 998
Bst2UI	CCWGG	4 cut(s)	341, 869, 1052, 1097
Bst4CI	ACNGT	9 cut(s)	56, 134, 541, 994, 1059, 1439, 1861, 2015, 2155
Bst6I	CTCTTC	2 cut(s)	1132, 1266
BstAFI	CTTAAG	1 cut(s)	1117
BstAPI	GCANNNNNTGC	1 cut(s)	2103
BstAUI	TGTACA	1 cut(s)	1059
BstBI	TTCGAA	1 cut(s)	1582
BstC8I	GCNNGC	4 cut(s)	120, 854, 1111, 2095
BstDSI	CCRYGG	2 cut(s)	75, 998
BstF5I	GGATG	7 cut(s)	221, 235, 733, 1377, 1537, 1711, 1876
BstHHI	GCGC	1 cut(s)	1219
BstKTI	GATC	6 cut(s)	754, 1247, 1317, 1361, 2046, 2127
BstMAI	GTCTC	2 cut(s)	2004, 2154
BstMBI	GATC	6 cut(s)	751, 1244, 1314, 1358, 2043, 2124
BstMWI	GCNNNNNNNGC	7 cut(s)	878, 977, 1106, 1413, 1594, 1667, 2103
BstNI	CCWGG	4 cut(s)	341, 869, 1052, 1097
BstNSI	RCATGY	2 cut(s)	928, 1729
BstSCI	CCNGG	4 cut(s)	339, 867, 1050, 1095
BstSFI	CTRYAG	1 cut(s)	2067
BstSLI	GKGCMC	1 cut(s)	1966
BstV1I	GCAGC	6 cut(s)	231, 515, 836, 1699, 1721, 2098
BstX2I	RGATCY	1 cut(s)	1244
BstYI	RGATCY	1 cut(s)	1244
BsuI	GTATCC	1 cut(s)	1934
BsuRI	GGCC	5 cut(s)	74, 852, 872, 1050, 1100
BtgI	CCRYGG	2 cut(s)	75, 998
BtgZI	GCGATG	1 cut(s)	1799
BtsCI	GGATG	7 cut(s)	221, 235, 733, 1377, 1537, 1711, 1876
BtsIMutI	CAGTG	5 cut(s)	537, 999, 1435, 1866, 2020
BveI	ACCTGC	1 cut(s)	2160
Cac8I	GCNNGC	4 cut(s)	120, 854, 1111, 2095
CaiI	CAGNNNCTG	1 cut(s)	1400
CfoI	GCGC	1 cut(s)	1219
Cfr10I	RCCGGY	1 cut(s)	852
Cfr13I	GGNCC	2 cut(s)	414, 850
Csp6I	GTAC	5 cut(s)	87, 509, 990, 1060, 1622
CviQI	GTAC	5 cut(s)	87, 509, 990, 1060, 1622
DpnI	GATC	6 cut(s)	753, 1246, 1316, 1360, 2045, 2126
DpnII	GATC	6 cut(s)	751, 1244, 1314, 1358, 2043, 2124
DraI	TTTAAA	2 cut(s)	145, 739
Eam1104I	CTCTTC	2 cut(s)	1132, 1266
EarI	CTCTTC	2 cut(s)	1132, 1266
Eco130I	CCWWGG	2 cut(s)	75, 998
Eco147I	AGGCCT	1 cut(s)	1050
Eco32I	GATATC	1 cut(s)	281
Eco47I	GGWCC	1 cut(s)	414
Eco57I	CTGAAG	1 cut(s)	1247
Eco88I	CYCGRG	1 cut(s)	2164
EcoO109I	RGGNCCY	1 cut(s)	414
EcoRI	GAATTC	1 cut(s)	1638
EcoRII	CCWGG	4 cut(s)	339, 867, 1050, 1095
EcoRV	GATATC	1 cut(s)	281
EcoT14I	CCWWGG	2 cut(s)	75, 998
EcoT22I	ATGCAT	5 cut(s)	165, 403, 613, 1485, 1522
ErhI	CCWWGG	2 cut(s)	75, 998
FalI	AAGNNNNNCTT	8 cut(s)	732, 764, 1111, 1143, 1245, 1277, 1338, 1370
FaqI	GGGAC	2 cut(s)	1074, 1363
FauNDI	CATATG	1 cut(s)	1021
FbaI	TGATCA	2 cut(s)	2043, 2124
Fnu4HI	GCNGC	8 cut(s)	220, 504, 519, 825, 1671, 1710, 1713, 2112
FokI	GGATG	7 cut(s)	228, 242, 740, 1384, 1544, 1718, 1863
Fsp4HI	GCNGC	8 cut(s)	220, 504, 519, 825, 1671, 1710, 1713, 2112
FspBI	CTAG	3 cut(s)	972, 1151, 1223
GlaI	GCGC	1 cut(s)	1218
GluI	GCNGC	8 cut(s)	220, 504, 519, 825, 1671, 1710, 1713, 2112
HaeIII	GGCC	5 cut(s)	74, 852, 872, 1050, 1100
HapII	CCGG	3 cut(s)	182, 787, 853
HhaI	GCGC	1 cut(s)	1219
Hin6I	GCGC	1 cut(s)	1217
HinP1I	GCGC	1 cut(s)	1217
HincII	GTYRAC	3 cut(s)	52, 922, 1723
HindII	GTYRAC	3 cut(s)	52, 922, 1723
HpaII	CCGG	3 cut(s)	182, 787, 853
HphI	GGTGA	5 cut(s)	1349, 1763, 1786, 1930, 2086
Hpy166II	GTNNAC	5 cut(s)	28, 52, 922, 1723, 2018
Hpy188I	TCNGA	6 cut(s)	9, 490, 837, 895, 1029, 1266
Hpy188III	TCNNGA	9 cut(s)	213, 749, 1091, 1223, 1274, 1411, 1652, 1880, 2128
Hpy8I	GTNNAC	5 cut(s)	28, 52, 922, 1723, 2018
HpyCH4III	ACNGT	9 cut(s)	56, 134, 541, 994, 1059, 1439, 1861, 2015, 2155
HpyCH4IV	ACGT	2 cut(s)	594, 1200
HpyF10VI	GCNNNNNNNGC	7 cut(s)	878, 977, 1106, 1413, 1594, 1667, 2103
HpySE526I	ACGT	2 cut(s)	594, 1200
HspAI	GCGC	1 cut(s)	1217
KroI	GCCGGC	1 cut(s)	852
KroNI	GCCGGC	1 cut(s)	854
Ksp22I	TGATCA	2 cut(s)	2043, 2124
Kzo9I	GATC	6 cut(s)	751, 1244, 1314, 1358, 2043, 2124
LguI	GCTCTTC	1 cut(s)	1266
LmnI	GCTCC	1 cut(s)	2204
Lsp1109I	GCAGC	6 cut(s)	231, 515, 836, 1699, 1721, 2098
MaeI	CTAG	3 cut(s)	972, 1151, 1223
MaeII	ACGT	2 cut(s)	594, 1200
MaeIII	GTNAC	6 cut(s)	94, 190, 388, 476, 1161, 1810
MalI	GATC	6 cut(s)	753, 1246, 1316, 1360, 2045, 2126
MboI	GATC	6 cut(s)	751, 1244, 1314, 1358, 2043, 2124
MboII	GAAGA	8 cut(s)	972, 1055, 1119, 1253, 1789, 1988, 2092, 2143
MflI	RGATCY	1 cut(s)	1244
MhlI	GDGCHC	1 cut(s)	1966
MlyI	GAGTC	3 cut(s)	1345, 2006, 2156
MmeI	TCCRAC	3 cut(s)	663, 886, 1283
Mph1103I	ATGCAT	5 cut(s)	165, 403, 613, 1485, 1522
MroNI	GCCGGC	1 cut(s)	852
MroXI	GAANNNNTTC	3 cut(s)	246, 794, 1034
MseI	TTAA	8 cut(s)	144, 270, 447, 738, 1118, 1421, 1574, 1766
MslI	CAYNNNNRTG	2 cut(s)	639, 845
MspA1I	CMGCKG	3 cut(s)	92, 887, 1712
MspCI	CTTAAG	1 cut(s)	1117
MspI	CCGG	3 cut(s)	182, 787, 853
MspR9I	CCNGG	4 cut(s)	341, 869, 1052, 1097
Mva1269I	GAATGC	2 cut(s)	1312, 1590
MvaI	CCWGG	4 cut(s)	341, 869, 1052, 1097
MwoI	GCNNNNNNNGC	7 cut(s)	878, 977, 1106, 1413, 1594, 1667, 2103
NaeI	GCCGGC	1 cut(s)	854
NcoI	CCATGG	2 cut(s)	75, 998
NdeI	CATATG	1 cut(s)	1021
NdeII	GATC	6 cut(s)	751, 1244, 1314, 1358, 2043, 2124
NgoMIV	GCCGGC	1 cut(s)	852
NlaIV	GGNNCC	3 cut(s)	415, 1246, 2174
NmeAIII	GCCGAG	1 cut(s)	181
NmuCI	GTSAC	1 cut(s)	190
NsiI	ATGCAT	5 cut(s)	165, 403, 613, 1485, 1522
NspI	RCATGY	2 cut(s)	928, 1729
NspV	TTCGAA	1 cut(s)	1582
PaeR7I	CTCGAG	1 cut(s)	2164
PceI	AGGCCT	1 cut(s)	1050
PciI	ACATGT	2 cut(s)	924, 1725
PciSI	GCTCTTC	1 cut(s)	1266
PctI	GAATGC	2 cut(s)	1312, 1590
PdiI	GCCGGC	1 cut(s)	854
PdmI	GAANNNNTTC	3 cut(s)	246, 794, 1034
PfeI	GAWTC	9 cut(s)	317, 890, 896, 1035, 1188, 1397, 1554, 1648, 1846
PflMI	CCANNNNNTGG	1 cut(s)	1886
PkrI	GCNGC	8 cut(s)	221, 505, 520, 826, 1672, 1711, 1714, 2113
PleI	GAGTC	3 cut(s)	1345, 2005, 2156
PpsI	GAGTC	3 cut(s)	1345, 2005, 2156
PpuMI	RGGWCCY	1 cut(s)	414
PscI	ACATGT	2 cut(s)	924, 1725
PshBI	ATTAAT	1 cut(s)	1574
PsiI	TTATAA	1 cut(s)	69
Psp1406I	AACGTT	2 cut(s)	594, 1200
Psp5II	RGGWCCY	1 cut(s)	414
Psp6I	CCWGG	4 cut(s)	339, 867, 1050, 1095
PspGI	CCWGG	4 cut(s)	339, 867, 1050, 1095
PspN4I	GGNNCC	3 cut(s)	415, 1246, 2174
PspPI	GGNCC	2 cut(s)	414, 850
PspPPI	RGGWCCY	1 cut(s)	414
PspXI	VCTCGAGB	1 cut(s)	2164
PsrI	GAACNNNNNNTAC	2 cut(s)	237, 269
PstNI	CAGNNNCTG	1 cut(s)	1400
PsuI	RGATCY	1 cut(s)	1244
PvuII	CAGCTG	3 cut(s)	92, 887, 1712
RsaI	GTAC	5 cut(s)	88, 510, 991, 1061, 1623
RsaNI	GTAC	5 cut(s)	87, 509, 990, 1060, 1622
RseI	CAYNNNNRTG	2 cut(s)	639, 845
SapI	GCTCTTC	1 cut(s)	1266
SaqAI	TTAA	8 cut(s)	144, 270, 447, 738, 1118, 1421, 1574, 1766
SatI	GCNGC	8 cut(s)	220, 504, 519, 825, 1671, 1710, 1713, 2112
Sau3AI	GATC	6 cut(s)	751, 1244, 1314, 1358, 2043, 2124
Sau96I	GGNCC	2 cut(s)	414, 850
ScaI	AGTACT	1 cut(s)	510
SchI	GAGTC	3 cut(s)	1345, 2006, 2156
ScrFI	CCNGG	4 cut(s)	341, 869, 1052, 1097
SduI	GDGCHC	1 cut(s)	1966
SfcI	CTRYAG	1 cut(s)	2067
Sfr274I	CTCGAG	1 cut(s)	2164
SfuI	TTCGAA	1 cut(s)	1582
SinI	GGWCC	1 cut(s)	414
SlaI	CTCGAG	1 cut(s)	2164
SmiMI	CAYNNNNRTG	2 cut(s)	639, 845
SmlI	CTYRAG	3 cut(s)	1117, 1411, 2164
SmoI	CTYRAG	3 cut(s)	1117, 1411, 2164
SseBI	AGGCCT	1 cut(s)	1050
SsiI	CCGC	2 cut(s)	519, 1671
SspI	AATATT	2 cut(s)	966, 1294
SspMI	CTAG	3 cut(s)	972, 1151, 1223
StuI	AGGCCT	1 cut(s)	1050
StyD4I	CCNGG	4 cut(s)	339, 867, 1050, 1095
StyI	CCWWGG	2 cut(s)	75, 998
TaaI	ACNGT	9 cut(s)	56, 134, 541, 994, 1059, 1439, 1861, 2015, 2155
TaiI	ACGT	2 cut(s)	597, 1203
TaqI	TCGA	7 cut(s)	419, 618, 1543, 1557, 1582, 1695, 2165
TatI	WGTACW	4 cut(s)	508, 989, 1059, 1621
TauI	GCSGC	2 cut(s)	521, 1673
TfiI	GAWTC	9 cut(s)	317, 890, 896, 1035, 1188, 1397, 1554, 1648, 1846
Tru1I	TTAA	8 cut(s)	144, 270, 447, 738, 1118, 1421, 1574, 1766
Tru9I	TTAA	8 cut(s)	144, 270, 447, 738, 1118, 1421, 1574, 1766
TscAI	CASTG	5 cut(s)	544, 999, 1442, 1866, 2020
TseFI	GTSAC	1 cut(s)	190
TseI	GCWGC	6 cut(s)	219, 503, 824, 1709, 1712, 2111
Tsp45I	GTSAC	1 cut(s)	190
TspGWI	ACGGA	1 cut(s)	749
TspRI	CASTG	5 cut(s)	544, 999, 1442, 1866, 2020
Van91I	CCANNNNNTGG	1 cut(s)	1886
Vha464I	CTTAAG	1 cut(s)	1117
VpaK11BI	GGWCC	1 cut(s)	414
VspI	ATTAAT	1 cut(s)	1574
XapI	RAATTY	5 cut(s)	491, 910, 1132, 1638, 2083
XbaI	TCTAGA	1 cut(s)	1222
XceI	RCATGY	2 cut(s)	928, 1729
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1883
XhoI	CTCGAG	1 cut(s)	2164
XmnI	GAANNNNTTC	3 cut(s)	246, 794, 1034
XspI	CTAG	3 cut(s)	972, 1151, 1223
ZrmI	AGTACT	1 cut(s)	510
Zsp2I	ATGCAT	5 cut(s)	165, 403, 613, 1485, 1522

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.