MD10G1285200.v1.1

Apoptosis inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004487
Physical Location & Seq
Reverse (-)
37512689 .. 37519779
7091 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1285200.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGGCTGACCCCTCCGGCGAGGCCAATCACATCGAGAAGCTTTACGAGTTCGGCGAACGACTGAACGAGTCCAAGGACAAGTCCCAGAATGTTAAGGATTACCAGGGGATTATCAATGCGGCCAAGACCAGTGTCAAGGCCAAGCAGCTGGCTGCCCAGCTTATTCCGAGGTTCTTCAAGTTCTTCCCCGAACTTTCGCAGCCCGCCATCGACACGCACCTCGATTTGATTGAAGAAGAAGAGCTCGGGGTACGAGTCCAAGCTATTCGGGGCCTTCCCCTGTTCTGTAAGGATACGCCTGAACATATTGCGAAAATTGTAGACATTCTTGTGCAGCTCCTTGCATCTGAGGAATTTGTGGAGCGTGATGCTGTGCATAAAGCACTTATGGCTCTTTTGAGGCAAGATGTTAAAGCTTCTTTGACAGCATTATTCAAGCACATTGGGAGTGCTGATGAACCAAGCACAGATGAGTTCATTCGGGAGAAGGTTTTAAGTTTCATTAGAGATAAGGTATTTCCTATTAAAGCTGAACTTTTGAAGCCTCAGGAAGAAATGGAAAGGCATATTACTGATTTGGTAAAAAAGAGTCTGGAAGATGTGACTGGTGCAGAGTTTAGAATGTTTATGGACTTCTTAAAAAGTTTGAGCATATTTGGGGAGAAAGCGCCTGCTGAACGCATGAAAGAACTTATTGGAATTATTGAAGGACAAGCTGATTTAGATGCCCAATTCAATGTTTCAGATGCTGATCATATTGATAGGTTGATATCCTGCCTATTCATGGCTCTGCCATTTGTTGTGAGGGGTGCGTCAAGCAGCAAATTCCTAAATTATCTGGACAAACACATCCTTCCGGTTTCGGACAAGCTTCCTGATGAAAGAAGGCTAGATTTGCTCAAAGCCCTTGCAGAAGTTTCACCTTTCACAGCACCACAAGATTCTCGTCAGATCCTTCCTTCTGTTGTTCAGCTGTTAAAGAAAAACATGCCTCGGAGAAAGACTGGAGATGAGTTTAACTTCACTTACGTAGAATGCTTATTGTTCACGTTCCACCATCTTGCTCACAAGGTTCCTAATGCTACTAACAGTCTATGCGGTTACAAGATAGTGACTGGGCAACCATCAGATAGACTTGGTGAGGACTTTTCAGAGCAATATAAGGAGTTTACTGAGAGGTTGAGTTATGTTGAGGAGCTAACTAGGGCCACCATGAAGAAATTAACACAGGGAATGGCTGAAAAGAACAAAGCGATGGCAGCTGCAAAATCTGATGAAGCAAAGGATGCCATTAAAACTGAAAAGCAGAATACTACAACTGGGTTGCGAACCTGCAATAACATATTGGCAATGACAAAGACTCTACATTCAAAAACACCCTCATTCATTGGAGACAAGAGCATCAACCTGTCTTGGAAAGAAGCAACAAAACCCGCTGTGCCCTCTAGCACACCTGTTGCAGGAGCAAAAAGAGCTGCTAATTTTTCCAATGGATCCGGAAATATGCCTTCAAAAAAGGGGCGTGGGGGCGGATTACAAAATCAACTTGTTAATAGAGCATTTGAAGGTTTATCTCAAGGTGGAAGAAGCGGGGGAAGGAGCGGTGGTGGTGGAAGGAGCAGAGGCAGAGGGTGGGGTGGCCGTGGAAGGGGTAGGGGTTACAGATAG

Protein Analysis

556

Amino Acids

61.66

Weight (kDa)

9.03

Isoelectric Point (pI)

44.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
API5 PF05918 11 - 553 3.7e-245 Apoptosis inhibitory protein 5 (API5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1340
AccBSI CCGCTC 1 cut(s) 1602
AccI GTMKAC 1 cut(s) 321
AccIII TCCGGA 1 cut(s) 1496
AciI CCGC 7 cut(s) 119, 204, 1098, 1434, 1530, 1590, 1602
AclWI GGATC 3 cut(s) 946, 1488, 1501
AcoI YGGCCR 2 cut(s) 120, 1639
AcsI RAATTY 2 cut(s) 353, 824
AfaI GTAC 1 cut(s) 252
AfiI CCNNNNNNNGG 3 cut(s) 784, 1460, 1648
AgsI TTSAA 9 cut(s) 178, 233, 436, 541, 707, 736, 1371, 1512, 1565
AjnI CCWGG 1 cut(s) 102
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 1 cut(s) 1386
AlwI GGATC 3 cut(s) 946, 1488, 1501
AlwNI CAGNNNCTG 1 cut(s) 749
Ama87I CYCGRG 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 1496
AoxI GGCC 6 cut(s) 21, 120, 138, 271, 1206, 1639
ApeKI GCWGC 8 cut(s) 145, 152, 199, 334, 819, 1259, 1262, 1475
ApoI RAATTY 2 cut(s) 353, 824
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 2 cut(s) 271, 1206
AsuHPI GGTGA 2 cut(s) 912, 1151
AvaI CYCGRG 1 cut(s) 245
AxyI CCTNAGG 1 cut(s) 546
BaeGI GKGCMC 1 cut(s) 1443
BamHI GGATCC 1 cut(s) 1493
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 8 cut(s) 139, 157, 211, 346, 831, 1249, 1271, 1462
BccI CCATC 4 cut(s) 215, 1065, 1132, 1249
BceAI ACGGC 1 cut(s) 1626
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 1 cut(s) 286
BclI TGATCA 1 cut(s) 751
BcoDI GTCTC 1 cut(s) 1386
BfaI CTAG 3 cut(s) 890, 1203, 1446
BfoI RGCGCY 1 cut(s) 671
BfuAI ACCTGC 1 cut(s) 1340
BfuI GTATCC 1 cut(s) 286
BisI GCNGC 9 cut(s) 120, 146, 153, 200, 335, 820, 1260, 1263, 1476
BlsI GCNGC 9 cut(s) 121, 147, 154, 201, 336, 821, 1261, 1264, 1477
Bme1390I CCNGG 1 cut(s) 104
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 2 cut(s) 271, 1206
BmiI GGNNCC 3 cut(s) 272, 1074, 1495
BmrFI CCNGG 1 cut(s) 104
BmrI ACTGGG 2 cut(s) 1125, 1329
BmsI GCATC 6 cut(s) 353, 358, 715, 736, 1276, 1410
BmuI ACTGGG 2 cut(s) 1125, 1329
BoxI GACNNNNGTC 1 cut(s) 131
BpmI CTGGAG 1 cut(s) 1026
BpuEI CTTGAG 1 cut(s) 1560
BsaAI YACGTR 1 cut(s) 1030
BsaBI GATNNNNATC 1 cut(s) 750
BsaJI CCNNGG 5 cut(s) 72, 103, 167, 992, 1642
BsaWI WCCGGW 2 cut(s) 856, 1496
BsaXI ACNNNNNCTCC 2 cut(s) 1591, 1621
Bsc4I CCNNNNNNNGG 3 cut(s) 784, 1460, 1648
Bse1I ACTGG 5 cut(s) 129, 610, 1009, 1120, 1324
Bse21I CCTNAGG 1 cut(s) 546
Bse3DI GCAATG 1 cut(s) 1356
Bse8I GATNNNNATC 1 cut(s) 750
BseAI TCCGGA 1 cut(s) 1496
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 5 cut(s) 72, 103, 167, 992, 1642
BseGI GGATG 2 cut(s) 849, 1291
BseJI GATNNNNATC 1 cut(s) 750
BseLI CCNNNNNNNGG 3 cut(s) 784, 1460, 1648
BseMI GCAATG 1 cut(s) 1356
BseMII CTCAG 3 cut(s) 339, 560, 1164
BseNI ACTGG 5 cut(s) 129, 610, 1009, 1120, 1324
BseRI GAGGAG 1 cut(s) 1208
BseSI GKGCMC 1 cut(s) 1443
BseXI GCAGC 8 cut(s) 139, 157, 211, 346, 831, 1249, 1271, 1462
BseYI CCCAGC 1 cut(s) 156
BsgI GTGCAG 2 cut(s) 353, 630
BshFI GGCC 6 cut(s) 23, 122, 140, 273, 1208, 1641
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 3 cut(s) 15, 857, 1497
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 3 cut(s) 784, 1460, 1648
BsmAI GTCTC 1 cut(s) 1386
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 1040
BsnI GGCC 6 cut(s) 23, 122, 140, 273, 1208, 1641
BsoBI CYCGRG 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 246, 1443
Bsp13I TCCGGA 1 cut(s) 1496
Bsp143I GATC 3 cut(s) 751, 951, 1493
BspACI CCGC 7 cut(s) 119, 204, 1098, 1434, 1530, 1590, 1602
BspANI GGCC 6 cut(s) 23, 122, 140, 273, 1208, 1641
BspCNI CTCAG 3 cut(s) 340, 559, 1165
BspEI TCCGGA 1 cut(s) 1496
BspLI GGNNCC 3 cut(s) 272, 1074, 1495
BspMI ACCTGC 1 cut(s) 1340
BspPI GGATC 3 cut(s) 946, 1488, 1501
BspQI GCTCTTC 1 cut(s) 234
BsrBI CCGCTC 1 cut(s) 1602
BsrDI GCAATG 1 cut(s) 1356
BsrI ACTGG 5 cut(s) 129, 610, 1009, 1120, 1324
BssECI CCNNGG 5 cut(s) 72, 103, 167, 992, 1642
BssMI GATC 3 cut(s) 751, 951, 1493
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 1091
Bst6I CTCTTC 1 cut(s) 234
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstBAI YACGTR 1 cut(s) 1030
BstC8I GCNNGC 3 cut(s) 150, 204, 672
BstDEI CTNAG 3 cut(s) 348, 546, 1173
BstDSI CCRYGG 1 cut(s) 1642
BstENI CCTNNNNNAGG 1 cut(s) 1458
BstF5I GGATG 2 cut(s) 849, 1291
BstH2I RGCGCY 1 cut(s) 671
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 3 cut(s) 754, 954, 1496
BstMAI GTCTC 1 cut(s) 1386
BstMBI GATC 3 cut(s) 751, 951, 1493
BstMWI GCNNNNNNNGC 5 cut(s) 389, 895, 1259, 1286, 1472
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 991
BstPAI GACNNNNGTC 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 102
BstSLI GKGCMC 1 cut(s) 1443
BstSNI TACGTA 1 cut(s) 1030
BstV1I GCAGC 8 cut(s) 139, 157, 211, 346, 831, 1249, 1271, 1462
BstX2I RGATCY 2 cut(s) 951, 1493
BstXI CCANNNNNNTGG 1 cut(s) 148
BstYI RGATCY 2 cut(s) 951, 1493
Bsu36I CCTNAGG 1 cut(s) 546
BsuI GTATCC 1 cut(s) 286
BsuRI GGCC 6 cut(s) 23, 122, 140, 273, 1208, 1641
BtgI CCRYGG 1 cut(s) 1642
BtgZI GCGATG 1 cut(s) 1268
BtsCI GGATG 2 cut(s) 849, 1291
BtsIMutI CAGTG 1 cut(s) 136
BveI ACCTGC 1 cut(s) 1340
Cac8I GCNNGC 3 cut(s) 150, 204, 672
CaiI CAGNNNCTG 1 cut(s) 749
CfoI GCGC 1 cut(s) 670
Cfr13I GGNCC 2 cut(s) 271, 1206
CseI GACGC 1 cut(s) 801
Csp6I GTAC 1 cut(s) 251
CviAII CATG 4 cut(s) 682, 784, 988, 1213
CviQI GTAC 1 cut(s) 251
DdeI CTNAG 3 cut(s) 348, 546, 1173
DpnI GATC 3 cut(s) 753, 953, 1495
DpnII GATC 3 cut(s) 751, 951, 1493
EaeI YGGCCR 2 cut(s) 120, 1639
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
EciI GGCGGA 1 cut(s) 1545
Ecl136II GAGCTC 1 cut(s) 244
Eco105I TACGTA 1 cut(s) 1030
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 246
Eco32I GATATC 1 cut(s) 771
Eco53kI GAGCTC 1 cut(s) 244
Eco81I CCTNAGG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 245
EcoICRI GAGCTC 1 cut(s) 244
EcoNI CCTNNNNNAGG 1 cut(s) 1458
EcoO109I RGGNCCY 1 cut(s) 271
EcoRII CCWGG 1 cut(s) 102
EcoRV GATATC 1 cut(s) 771
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 4 cut(s) 685, 787, 991, 1216
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FaqI GGGAC 1 cut(s) 67
FatI CATG 4 cut(s) 681, 783, 987, 1212
FauI CCCGC 3 cut(s) 211, 1441, 1583
FbaI TGATCA 1 cut(s) 751
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 9 cut(s) 120, 146, 153, 200, 335, 820, 1260, 1263, 1476
FokI GGATG 2 cut(s) 836, 1298
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 9 cut(s) 120, 146, 153, 200, 335, 820, 1260, 1263, 1476
FspBI CTAG 3 cut(s) 890, 1203, 1446
GlaI GCGC 1 cut(s) 669
GluI GCNGC 9 cut(s) 120, 146, 153, 200, 335, 820, 1260, 1263, 1476
GsaI CCCAGC 1 cut(s) 160
GsuI CTGGAG 1 cut(s) 1026
HaeII RGCGCY 1 cut(s) 671
HaeIII GGCC 6 cut(s) 23, 122, 140, 273, 1208, 1641
HapII CCGG 3 cut(s) 15, 857, 1497
HgaI GACGC 1 cut(s) 801
HhaI GCGC 1 cut(s) 670
Hin1II CATG 4 cut(s) 685, 787, 991, 1216
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HindIII AAGCTT 3 cut(s) 38, 414, 869
HinfI GANTC 5 cut(s) 68, 255, 589, 941, 1360
HpaII CCGG 3 cut(s) 15, 857, 1497
HphI GGTGA 2 cut(s) 912, 1151
Hpy166II GTNNAC 3 cut(s) 322, 1047, 1170
Hpy188I TCNGA 9 cut(s) 168, 349, 745, 865, 951, 996, 1129, 1153, 1273
Hpy188III TCNNGA 7 cut(s) 34, 482, 548, 593, 839, 875, 1497
Hpy8I GTNNAC 3 cut(s) 322, 1047, 1170
HpyCH4III ACNGT 1 cut(s) 1091
HpyCH4IV ACGT 2 cut(s) 1029, 1049
HpyCH4V TGCA 8 cut(s) 334, 344, 376, 611, 911, 1265, 1335, 1460
HpyF10VI GCNNNNNNNGC 5 cut(s) 389, 895, 1259, 1286, 1472
HpyF3I CTNAG 3 cut(s) 348, 546, 1173
HpySE526I ACGT 2 cut(s) 1029, 1049
Hsp92II CATG 4 cut(s) 685, 787, 991, 1216
HspAI GCGC 1 cut(s) 668
Kpn2I TCCGGA 1 cut(s) 1496
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 3 cut(s) 751, 951, 1493
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 6 cut(s) 342, 361, 1195, 1463, 1599, 1617
Lsp1109I GCAGC 8 cut(s) 139, 157, 211, 346, 831, 1249, 1271, 1462
LweI GCATC 6 cut(s) 353, 358, 715, 736, 1276, 1410
MaeI CTAG 3 cut(s) 890, 1203, 1446
MaeII ACGT 2 cut(s) 1029, 1049
MaeIII GTNAC 4 cut(s) 601, 1100, 1111, 1658
MalI GATC 3 cut(s) 753, 953, 1495
MbiI CCGCTC 1 cut(s) 1602
MboI GATC 3 cut(s) 751, 951, 1493
MboII GAAGA 9 cut(s) 166, 175, 245, 248, 251, 563, 608, 1228, 1596
MflI RGATCY 2 cut(s) 951, 1493
MhlI GDGCHC 2 cut(s) 246, 1443
MluCI AATT 8 cut(s) 315, 353, 699, 731, 824, 832, 1220, 1480
MlyI GAGTC 4 cut(s) 77, 264, 598, 1354
MroI TCCGGA 1 cut(s) 1496
MslI CAYNNNNRTG 1 cut(s) 329
MspA1I CMGCKG 4 cut(s) 148, 973, 1262, 1436
MspI CCGG 3 cut(s) 15, 857, 1497
MspR9I CCNGG 1 cut(s) 104
Mva1269I GAATGC 1 cut(s) 1040
MvaI CCWGG 1 cut(s) 104
MwoI GCNNNNNNNGC 5 cut(s) 389, 895, 1259, 1286, 1472
NdeII GATC 3 cut(s) 751, 951, 1493
NlaIII CATG 4 cut(s) 685, 787, 991, 1216
NlaIV GGNNCC 3 cut(s) 272, 1074, 1495
NmuCI GTSAC 2 cut(s) 601, 1111
NspI RCATGY 1 cut(s) 991
PciSI GCTCTTC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 1 cut(s) 1040
PfeI GAWTC 1 cut(s) 941
PkrI GCNGC 9 cut(s) 121, 147, 154, 201, 336, 821, 1261, 1264, 1477
PleI GAGTC 4 cut(s) 76, 263, 597, 1354
PpsI GAGTC 4 cut(s) 76, 263, 597, 1354
Ppu21I YACGTR 1 cut(s) 1030
PshAI GACNNNNGTC 1 cut(s) 131
Psp124BI GAGCTC 1 cut(s) 246
Psp6I CCWGG 1 cut(s) 102
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 3 cut(s) 272, 1074, 1495
PspPI GGNCC 2 cut(s) 271, 1206
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 2 cut(s) 951, 1493
PvuII CAGCTG 3 cut(s) 148, 973, 1262
RsaI GTAC 1 cut(s) 252
RsaNI GTAC 1 cut(s) 251
RseI CAYNNNNRTG 1 cut(s) 329
SacI GAGCTC 1 cut(s) 246
SapI GCTCTTC 1 cut(s) 234
SatI GCNGC 9 cut(s) 120, 146, 153, 200, 335, 820, 1260, 1263, 1476
Sau3AI GATC 3 cut(s) 751, 951, 1493
Sau96I GGNCC 2 cut(s) 271, 1206
SchI GAGTC 4 cut(s) 77, 264, 598, 1354
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 2 cut(s) 246, 1443
SfaNI GCATC 6 cut(s) 353, 358, 715, 736, 1276, 1410
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 1575
SmoI CTYRAG 1 cut(s) 1575
SnaBI TACGTA 1 cut(s) 1030
Sse9I AATT 8 cut(s) 315, 353, 699, 731, 824, 832, 1220, 1480
SsiI CCGC 7 cut(s) 119, 204, 1098, 1434, 1530, 1590, 1602
SspMI CTAG 3 cut(s) 890, 1203, 1446
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 1091
TaiI ACGT 2 cut(s) 1032, 1052
TaqI TCGA 3 cut(s) 33, 210, 222
TasI AATT 8 cut(s) 315, 353, 699, 731, 824, 832, 1220, 1480
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 1 cut(s) 941
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 2 cut(s) 601, 1111
TseI GCWGC 8 cut(s) 145, 152, 199, 334, 819, 1259, 1262, 1475
Tsp45I GTSAC 2 cut(s) 601, 1111
TspDTI ATGAA 9 cut(s) 466, 471, 490, 698, 772, 894, 1229, 1290, 1375
TspRI CASTG 1 cut(s) 136
XagI CCTNNNNNAGG 1 cut(s) 1458
XapI RAATTY 2 cut(s) 353, 824
XceI RCATGY 1 cut(s) 991
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 3 cut(s) 890, 1203, 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.