FvH4_3g12360

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008257
Physical Location & Seq
Reverse (-)
7335415 .. 7338857
3443 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g12360.t2

Sequence Viewer

Length: 1479 bp
ATGAGCCCCACTGCTGAGCAGATTCTGGACATTGTACAGAACAAAGCTGATAGAGTCAAAATTTGGGAAGTTCTTGACACTCTTCCAGCCTCTCATATCATATGGGATGACATCATTAATGTAGCTGTTCAACTTCGTCTCAATAAAATGTGGGATTCCATAATTTTGATATGTGAGTGGATCTTGCACAAGAGCTCCTACAAGCCAGATGTAATCTGCTACAATTTACTGCTTGATGCTTATGGGCAGAAATCACAGCATAAAGATGCAGAATCCACATATTTAGAACTTCTTGAAGCTCGATGCATTCCCACCGAGGACACTTATGCCCTTCTTATAAAGGCCTACTGCAAGTCTGGATTGCTTGATAGGGCTGAAGCCACTTTTGGTGAAATGCGAAAGTATGGTCTTCCTCCAAGTGCAATTGTGTTCGATGCTTATATTAATGGGTTGATAAAAGGAGGAAACCCACAAAAAGCAGAAGAGATCTTTCAGAGGATGAAAAGAGATCAATGTGAACCTTCTGCTGACACTTATACTATGTTAATCAATATATATGGGAAGGCAAGAAAATCCTATATGGCTCTAAAACTGTTCCGTGAAATGAGAAGTCAGAAATGTAAACCGAACATATGCACATATACTGCTTTGATAAATGCATTTGCTAGGGAAGGACATTGTGAGAAAGCAGAAGAAATATTTGAGCAGCTACAAGAGGCAGGGCTTGAGCCTGATGTATATGCTTATAACGCCCTTATGGAAGCCTACAGTCGAGCAGGATATCCTTATGGAGCTGCAGAAATTTTCTCTTTGATGCAACACATGGGATGTGAACCAGATAGAGCTTCATATAATATCATGGTAGATGCATATGGTAGAGGTGGTCTGCATGAAGACGCGCAAGCGGCATTTGAAGAGATGAAGCGATTAGGCATAACCCCAACAATCAAATCCCACATGCTACTTTTATCCGCGTACTCCAAAGCTGGGAATGTAGCTAAGTGTGAAGAAATTGTGAACCAGATGCAAGAATCTGGTTTGGCACTAGACACATTTGTGATTAACAGTATGCTAAACCTGTATGGCCGGTTAGGACAGCTAGAAAAAATGGAGGAAGTTTTGACTGCCCTGGAAAAAGGACCATATGCAGCTGATATCAGTACATACAATATCTTGATCAACATATATGGAAGGGCAGGATTTTTTGACAAGATGGAAGAGCTCTTTCAATCACTTCCATTAAAAAAGTTAAAACCTGATGTTGTGACATGGACTTCTCGTCTTGGAGCCTACTCTAAGAAGAAACTATACACAAGATGCTTGGAAATCTTTGAAGAAATGATTGATGCCGGTTGCTATCCAGATGGCGGAACTGCCAAAGTACTTCTTGCTTCCTGCTCAAGCAAAGATCAAACCCAGCAGGTTAGTACTGTTATTAGAACAATGCACAAGGATGTGAGGACTGTCTTACCTATCTGA

Protein Analysis

493

Amino Acids

55.61

Weight (kDa)

5.68

Isoelectric Point (pI)

39.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 154 - 203 6.9e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 155 - 204 3.8e-10 PPR repeat family
PPR_1 PF12854 188 - 219 2.8e-07 PPR repeat
PPR_2 PF13041 194 - 228 9.9e-06 PPR repeat family
PPR PF01535 194 - 223 1.9e-06 PPR repeat
PPR_3 PF13812 217 - 274 1.5e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 225 - 271 6.7e-10 PPR repeat family
PPR_long PF17177 245 - 346 4.2e-12 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 264 - 309 9.8e-13 PPR repeat family
PPR PF01535 264 - 293 5.3e-06 PPR repeat
PPR_3 PF13812 275 - 307 9.1e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 293 - 322 5.6e-08 PPR repeat
PPR_2 PF13041 295 - 341 2.4e-12 PPR repeat family
PPR PF01535 299 - 328 3e-07 PPR repeat
TPR_24 PF23276 308 - 390 2.1e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 319 - 378 6.4e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 330 - 377 7.7e-12 PPR repeat family
PPR_long PF17177 335 - 465 1.2e-09 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 343 - 455 2.3e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 361 - 394 7.6e-07 PPR repeat
PPR PF01535 369 - 398 3.7e-07 PPR repeat
PPR_2 PF13041 369 - 407 4.9e-06 PPR repeat family
PPR_2 PF13041 471 - 512 6.9e-07 PPR repeat family
PPR_2 PF13041 505 - 552 2.6e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 338, 747
Acc36I ACCTGC 1 cut(s) 1411
AccII CGCG 2 cut(s) 899, 974
AciI CCGC 3 cut(s) 905, 972, 1368
AclWI GGATC 1 cut(s) 188
AcoI YGGCCR 1 cut(s) 1084
AcsI RAATTY 2 cut(s) 60, 801
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 5 cut(s) 36, 977, 1162, 1383, 1429
AfiI CCNNNNNNNGG 2 cut(s) 987, 1367
AgsI TTSAA 5 cut(s) 131, 296, 914, 1229, 1334
AhdI GACNNNNNGTC 1 cut(s) 1278
AjnI CCWGG 1 cut(s) 1128
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
AleI CACNNNNGTG 1 cut(s) 1055
Alw21I GWGCWC 2 cut(s) 197, 1224
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 1 cut(s) 188
AlwNI CAGNNNCTG 1 cut(s) 25
AoxI GGCC 2 cut(s) 342, 1084
ApeKI GCWGC 3 cut(s) 706, 794, 1148
ApoI RAATTY 2 cut(s) 60, 801
ArsI GACNNNNNNTTYG 2 cut(s) 391, 423
AseI ATTAAT 2 cut(s) 117, 444
AspLEI GCGC 1 cut(s) 901
AspS9I GGNCC 1 cut(s) 1139
AsuHPI GGTGA 1 cut(s) 401
AvaII GGWCC 1 cut(s) 1139
BanII GRGCYC 3 cut(s) 8, 197, 1224
BarI GAAGNNNNNNTAC 2 cut(s) 1292, 1324
BbsI GAAGAC 2 cut(s) 401, 900
Bbv12I GWGCWC 2 cut(s) 197, 1224
BbvI GCAGC 3 cut(s) 718, 781, 1160
BccI CCATC 2 cut(s) 1207, 1358
BciT130I CCWGG 1 cut(s) 1130
BclI TGATCA 1 cut(s) 1176
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 3 cut(s) 666, 1046, 1100
BfmI CTRYAG 2 cut(s) 766, 795
BfuAI ACCTGC 1 cut(s) 1411
BglII AGATCT 1 cut(s) 486
BisI GCNGC 4 cut(s) 707, 795, 906, 1149
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 4 cut(s) 708, 796, 907, 1150
BmcAI AGTACT 2 cut(s) 1383, 1429
Bme1390I CCNGG 1 cut(s) 1130
Bme18I GGWCC 1 cut(s) 1139
BmeRI GACNNNNNGTC 1 cut(s) 1278
BmgT120I GGNCC 1 cut(s) 1139
BmiI GGNNCC 1 cut(s) 1288
BmrFI CCNGG 1 cut(s) 1130
BmsI GCATC 9 cut(s) 226, 256, 293, 424, 804, 856, 1014, 1307, 1336
BpiI GAAGAC 2 cut(s) 401, 900
Bpu1102I GCTNAGC 1 cut(s) 15
BpuEI CTTGAG 2 cut(s) 746, 1384
BsaJI CCNNGG 2 cut(s) 315, 1128
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 2 cut(s) 987, 1367
Bse118I RCCGGY 2 cut(s) 1086, 1349
BseBI CCWGG 1 cut(s) 1130
BseDI CCNNGG 2 cut(s) 315, 1128
BseGI GGATG 4 cut(s) 112, 504, 833, 1459
BseLI CCNNNNNNNGG 2 cut(s) 987, 1367
BseMII CTCAG 1 cut(s) 6
BseXI GCAGC 3 cut(s) 718, 781, 1160
BseYI CCCAGC 2 cut(s) 986, 1416
Bsh1236I CGCG 2 cut(s) 899, 974
BshFI GGCC 2 cut(s) 344, 1086
BsiHKAI GWGCWC 2 cut(s) 197, 1224
BsiSI CCGG 2 cut(s) 1087, 1350
BslI CCNNNNNNNGG 2 cut(s) 987, 1367
BsmAI GTCTC 1 cut(s) 143
BsmBI CGTCTC 1 cut(s) 143
BsmI GAATGC 1 cut(s) 306
BsnI GGCC 2 cut(s) 344, 1086
Bsp1286I GDGCHC 3 cut(s) 8, 197, 1224
Bsp1407I TGTACA 1 cut(s) 34
Bsp143I GATC 5 cut(s) 180, 486, 508, 1176, 1408
Bsp1720I GCTNAGC 1 cut(s) 15
BspACI CCGC 3 cut(s) 905, 972, 1368
BspANI GGCC 2 cut(s) 344, 1086
BspCNI CTCAG 1 cut(s) 7
BspFNI CGCG 2 cut(s) 899, 974
BspLI GGNNCC 1 cut(s) 1288
BspMAI CTGCAG 1 cut(s) 799
BspMI ACCTGC 1 cut(s) 1411
BspPI GGATC 1 cut(s) 188
BspQI GCTCTTC 1 cut(s) 1212
BsrFI RCCGGY 2 cut(s) 1086, 1349
BsrGI TGTACA 1 cut(s) 34
BssAI RCCGGY 2 cut(s) 1086, 1349
BssECI CCNNGG 2 cut(s) 315, 1128
BssMI GATC 5 cut(s) 180, 486, 508, 1176, 1408
Bst2UI CCWGG 1 cut(s) 1130
Bst4CI ACNGT 5 cut(s) 594, 770, 1067, 1432, 1465
Bst6I CTCTTC 4 cut(s) 87, 477, 909, 1212
BstAUI TGTACA 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 903
BstDEI CTNAG 3 cut(s) 15, 999, 1296
BstF5I GGATG 4 cut(s) 112, 504, 833, 1459
BstFNI CGCG 2 cut(s) 899, 974
BstHHI GCGC 1 cut(s) 901
BstKTI GATC 5 cut(s) 183, 489, 511, 1179, 1411
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 5 cut(s) 180, 486, 508, 1176, 1408
BstMWI GCNNNNNNNGC 2 cut(s) 749, 905
BstNI CCWGG 1 cut(s) 1130
BstNSI RCATGY 1 cut(s) 961
BstSCI CCNGG 1 cut(s) 1128
BstSFI CTRYAG 2 cut(s) 766, 795
BstUI CGCG 2 cut(s) 899, 974
BstV1I GCAGC 3 cut(s) 718, 781, 1160
BstV2I GAAGAC 2 cut(s) 401, 900
BstX2I RGATCY 2 cut(s) 180, 486
BstYI RGATCY 2 cut(s) 180, 486
BsuRI GGCC 2 cut(s) 344, 1086
BtsCI GGATG 4 cut(s) 112, 504, 833, 1459
BtsI GCAGTG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 9
BveI ACCTGC 1 cut(s) 1411
Cac8I GCNNGC 1 cut(s) 903
CaiI CAGNNNCTG 1 cut(s) 25
CfoI GCGC 1 cut(s) 901
Cfr10I RCCGGY 2 cut(s) 1086, 1349
Cfr13I GGNCC 1 cut(s) 1139
CseI GACGC 1 cut(s) 905
Csp6I GTAC 5 cut(s) 35, 976, 1161, 1382, 1428
CviAII CATG 5 cut(s) 823, 859, 890, 958, 1269
CviQI GTAC 5 cut(s) 35, 976, 1161, 1382, 1428
DdeI CTNAG 3 cut(s) 15, 999, 1296
DpnI GATC 5 cut(s) 182, 488, 510, 1178, 1410
DpnII GATC 5 cut(s) 180, 486, 508, 1176, 1408
DriI GACNNNNNGTC 1 cut(s) 1278
EaeI YGGCCR 1 cut(s) 1084
Eam1104I CTCTTC 4 cut(s) 87, 477, 909, 1212
Eam1105I GACNNNNNGTC 1 cut(s) 1278
EarI CTCTTC 4 cut(s) 87, 477, 909, 1212
EciI GGCGGA 1 cut(s) 1383
Ecl136II GAGCTC 2 cut(s) 195, 1222
Eco147I AGGCCT 1 cut(s) 344
Eco24I GRGCYC 3 cut(s) 8, 197, 1224
Eco32I GATATC 2 cut(s) 782, 1156
Eco47I GGWCC 1 cut(s) 1139
Eco53kI GAGCTC 2 cut(s) 195, 1222
Eco57I CTGAAG 1 cut(s) 396
EcoICRI GAGCTC 2 cut(s) 195, 1222
EcoRII CCWGG 1 cut(s) 1128
EcoRV GATATC 2 cut(s) 782, 1156
EcoT22I ATGCAT 3 cut(s) 308, 661, 871
EcoT38I GRGCYC 3 cut(s) 8, 197, 1224
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 5 cut(s) 826, 862, 893, 961, 1272
FalI AAGNNNNNCTT 2 cut(s) 1371, 1403
FatI CATG 5 cut(s) 822, 858, 889, 957, 1268
FauNDI CATATG 4 cut(s) 101, 632, 871, 1144
FbaI TGATCA 1 cut(s) 1176
Fnu4HI GCNGC 4 cut(s) 707, 795, 906, 1149
FokI GGATG 4 cut(s) 119, 511, 840, 1466
FriOI GRGCYC 3 cut(s) 8, 197, 1224
Fsp4HI GCNGC 4 cut(s) 707, 795, 906, 1149
FspBI CTAG 3 cut(s) 666, 1046, 1100
GlaI GCGC 1 cut(s) 900
GluI GCNGC 4 cut(s) 707, 795, 906, 1149
GsaI CCCAGC 2 cut(s) 990, 1420
HaeIII GGCC 2 cut(s) 344, 1086
HapII CCGG 2 cut(s) 1087, 1350
HgaI GACGC 1 cut(s) 905
HhaI GCGC 1 cut(s) 901
Hin1II CATG 5 cut(s) 826, 862, 893, 961, 1272
Hin6I GCGC 1 cut(s) 899
HinP1I GCGC 1 cut(s) 899
HinfI GANTC 5 cut(s) 22, 54, 155, 272, 1031
HpaII CCGG 2 cut(s) 1087, 1350
HphI GGTGA 1 cut(s) 401
Hpy166II GTNNAC 4 cut(s) 518, 623, 833, 1018
Hpy188I TCNGA 3 cut(s) 495, 615, 1478
Hpy188III TCNNGA 6 cut(s) 26, 74, 293, 357, 1174, 1361
Hpy8I GTNNAC 4 cut(s) 518, 623, 833, 1018
HpyAV CCTTC 5 cut(s) 341, 531, 556, 665, 1185
HpyCH4III ACNGT 5 cut(s) 594, 770, 1067, 1432, 1465
HpyF10VI GCNNNNNNNGC 2 cut(s) 749, 905
HpyF3I CTNAG 3 cut(s) 15, 999, 1296
Hsp92II CATG 5 cut(s) 826, 862, 893, 961, 1272
HspAI GCGC 1 cut(s) 899
Ksp22I TGATCA 1 cut(s) 1176
Kzo9I GATC 5 cut(s) 180, 486, 508, 1176, 1408
LguI GCTCTTC 1 cut(s) 1212
LmnI GCTCC 3 cut(s) 200, 791, 1286
Lsp1109I GCAGC 3 cut(s) 718, 781, 1160
LweI GCATC 9 cut(s) 226, 256, 293, 424, 804, 856, 1014, 1307, 1336
MaeI CTAG 3 cut(s) 666, 1046, 1100
MaeIII GTNAC 1 cut(s) 1264
MalI GATC 5 cut(s) 182, 488, 510, 1178, 1410
MboI GATC 5 cut(s) 180, 486, 508, 1176, 1408
MfeI CAATTG 1 cut(s) 423
MflI RGATCY 2 cut(s) 180, 486
MhlI GDGCHC 3 cut(s) 8, 197, 1224
MluCI AATT 6 cut(s) 60, 162, 223, 423, 801, 1011
MlyI GAGTC 1 cut(s) 63
MnlI CCTC 9 cut(s) 100, 310, 423, 455, 489, 709, 872, 1105, 1452
Mph1103I ATGCAT 3 cut(s) 308, 661, 871
MseI TTAA 6 cut(s) 117, 444, 545, 1062, 1241, 1250
MslI CAYNNNNRTG 3 cut(s) 264, 1055, 1452
MspA1I CMGCKG 1 cut(s) 1151
MspI CCGG 2 cut(s) 1087, 1350
MspR9I CCNGG 1 cut(s) 1130
MunI CAATTG 1 cut(s) 423
Mva1269I GAATGC 1 cut(s) 306
MvaI CCWGG 1 cut(s) 1130
MvnI CGCG 2 cut(s) 899, 974
MwoI GCNNNNNNNGC 2 cut(s) 749, 905
NdeI CATATG 4 cut(s) 101, 632, 871, 1144
NdeII GATC 5 cut(s) 180, 486, 508, 1176, 1408
NlaIII CATG 5 cut(s) 826, 862, 893, 961, 1272
NlaIV GGNNCC 1 cut(s) 1288
NmuCI GTSAC 1 cut(s) 1264
NsiI ATGCAT 3 cut(s) 308, 661, 871
NspI RCATGY 1 cut(s) 961
OliI CACNNNNGTG 1 cut(s) 1055
PceI AGGCCT 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 1212
PctI GAATGC 1 cut(s) 306
PfeI GAWTC 4 cut(s) 22, 155, 272, 1031
PkrI GCNGC 4 cut(s) 708, 796, 907, 1150
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PshBI ATTAAT 2 cut(s) 117, 444
PsiI TTATAA 2 cut(s) 338, 747
Psp124BI GAGCTC 2 cut(s) 197, 1224
Psp6I CCWGG 1 cut(s) 1128
PspFI CCCAGC 2 cut(s) 986, 1416
PspGI CCWGG 1 cut(s) 1128
PspN4I GGNNCC 1 cut(s) 1288
PspPI GGNCC 1 cut(s) 1139
PstI CTGCAG 1 cut(s) 799
PstNI CAGNNNCTG 1 cut(s) 25
PsuI RGATCY 2 cut(s) 180, 486
PvuII CAGCTG 1 cut(s) 1151
RsaI GTAC 5 cut(s) 36, 977, 1162, 1383, 1429
RsaNI GTAC 5 cut(s) 35, 976, 1161, 1382, 1428
RseI CAYNNNNRTG 3 cut(s) 264, 1055, 1452
SacI GAGCTC 2 cut(s) 197, 1224
SapI GCTCTTC 1 cut(s) 1212
SaqAI TTAA 6 cut(s) 117, 444, 545, 1062, 1241, 1250
SatI GCNGC 4 cut(s) 707, 795, 906, 1149
Sau3AI GATC 5 cut(s) 180, 486, 508, 1176, 1408
Sau96I GGNCC 1 cut(s) 1139
ScaI AGTACT 2 cut(s) 1383, 1429
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 1130
SduI GDGCHC 3 cut(s) 8, 197, 1224
SfaNI GCATC 9 cut(s) 226, 256, 293, 424, 804, 856, 1014, 1307, 1336
SfcI CTRYAG 2 cut(s) 766, 795
SinI GGWCC 1 cut(s) 1139
SmiMI CAYNNNNRTG 3 cut(s) 264, 1055, 1452
SmlI CTYRAG 2 cut(s) 725, 1399
SmoI CTYRAG 2 cut(s) 725, 1399
Sse9I AATT 6 cut(s) 60, 162, 223, 423, 801, 1011
SseBI AGGCCT 1 cut(s) 344
SsiI CCGC 3 cut(s) 905, 972, 1368
SspI AATATT 1 cut(s) 699
SspMI CTAG 3 cut(s) 666, 1046, 1100
SstI GAGCTC 2 cut(s) 197, 1224
StuI AGGCCT 1 cut(s) 344
StyD4I CCNGG 1 cut(s) 1128
TaaI ACNGT 5 cut(s) 594, 770, 1067, 1432, 1465
TaqI TCGA 3 cut(s) 301, 432, 772
TasI AATT 6 cut(s) 60, 162, 223, 423, 801, 1011
TatI WGTACW 4 cut(s) 34, 1160, 1381, 1427
TauI GCSGC 1 cut(s) 908
TfiI GAWTC 4 cut(s) 22, 155, 272, 1031
Tru1I TTAA 6 cut(s) 117, 444, 545, 1062, 1241, 1250
Tru9I TTAA 6 cut(s) 117, 444, 545, 1062, 1241, 1250
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 1 cut(s) 1264
TseI GCWGC 3 cut(s) 706, 794, 1148
Tsp45I GTSAC 1 cut(s) 1264
TspDTI ATGAA 4 cut(s) 515, 837, 906, 935
TspGWI ACGGA 1 cut(s) 587
TspRI CASTG 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 1139
VspI ATTAAT 2 cut(s) 117, 444
XapI RAATTY 2 cut(s) 60, 801
XceI RCATGY 1 cut(s) 961
XspI CTAG 3 cut(s) 666, 1046, 1100
ZrmI AGTACT 2 cut(s) 1383, 1429
Zsp2I ATGCAT 3 cut(s) 308, 661, 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.