Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008257

Physical Location & Seq

Genomic Coordinates Reverse (-)

32824696 .. 32828175

Gene Structure 3480 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1231700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGCCATCAAACACTATCTTCTTCTCACAAACTGGAAAGCTCCTCCTTCTTCTCCAATTTCCAAACATTCATAGTGATGCAAGATGGAAAGCCAAGAGTTCCAGTAGTAGAGCTGTTGAGAAGAGCAAGGGCGACGGACTTTACATTGACAAACGCGGAAAATTCAGAAGCTTCAATAACAAGAAACTGTCTAGGAAACGATGTGGTTCTCTAAGGGGCCGAGGATGGAAATATGGATCGGGGTTTGTGGACGGAGTTTTCCCTGTGATGAGCCCCACTGGTCAGCAGATTCTGGACTTTGTGCAGAAGGAAGTCGATCGAAACAGTATTTGGGAAATTCTTGACACTCTTCCAGCCTCTCATACCATATGGGACGATATCATTAACGTAGCTGTTCAGCTTCGTCTCAACAAACAGTGGGGTTCCATAATCTTGATATGTGAGTGGATATTGTACAAGAGCTCCTTCAAGCCAGATGTCATCTGCTACAATTTGCTTATCGACGCTTATGGGCAGAAGTCGCAACATAAAGATGCAGAATCGACATATTTGGAACTTCTTGAAGCTCGATGCATACCTACTGAAGACACCTATGCCCTTCTTTTAAAGGCCTACTGCAAGTCTGGGTTGTTCGATAAGGCTGAAGCCATATTTGGCGAAATGCGGAAGTATGGTCTTCCACCAAGTGCAATTGTGTTCGATGCTTACATCAACGGGTTACTAAAAGGCGGAGACCCTCAAAAAGCAGTAGAGATCTTTCAGAGGATGAAGAGAGATCAATGCCAGCCATCTACTGAGACGTATACGATGCTAATCAATTTATACGGAAAGGAAAGGAAATCTTTTATGGCTCTAAAGATGTTTCGAGAAATGAAAAGCCAGAAATGTAGACCCAACATATGCACGTATACAGCTTTGGTAAATGCATTTGCGAGGGACGGACTTTGTGAGAAAGCGGAAGAAATTTTTGAGCAGATGCAAGGAGCAGGGTATGAGCCTGATGTGTATGCGTACAATGCCCTTATGGAAGCTTACAGTCGTGCAGGGTTTCCTTACGGAGCTGCAGAAATTTTCTCTCTAATGCAGCACATGGGATGTGAACCCGATAGAGCTTCGTATAATATCATGGTAGATGCATATGGGAGAGCTGGTCTTCATGAAGATGCACAAGCTGCGTTTGAAGAGATGAAGCGGTTAGGAATAACCCCGACAGTGAAATCCCATATGCTACTTTTATCCGCCTACACCAAAGCTGGCAACGTATCAAAATGCGAGGACATTGTGAACCAGATGCAAGAGTCCGGGGTGGAGCTAGACACTTTCGTCATTAACAGCATGTTAAATCTGTATGGCCGGTTAGGCCAGTTAGAGAAGATGGAGGAAGTTTTGACAGCAATGGAAACAGGACCCTACGCAGCTGATATCAGTACATACAACATCTTGATTAACATATATGGACGGGCAGGATTTTTCGACAAGATGGAAGAGCTCTTTCAGTCGCTTCCACTCAAAAACTTGAAACCGGATGTTGTGACATGGACTTCGCGTCTTGGAGCATACTCGAGAAAGAAACTATACACGAGATGCTTGGAAATTTTCGAAGAAATGATTGATGCCGGTTGCAATCCAGATGCCGGAACTGCCAGGGTACTCCTTGGAGCGTGTTCAAGCGACGATCAAACCGAGCAGGTTAGTACTGTTATTAGAAGCATGCACAAGGAGGTCAGGACTGTTTTACCTATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

582

Amino Acids

65.66

Weight (kDa)

7.44

Isoelectric Point (pI)

39.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	155 - 206	3.9e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	158 - 207	2.9e-10	PPR repeat family
PPR_1	PF12854	191 - 222	2.4e-06	PPR repeat
PPR_2	PF13041	197 - 231	3.2e-06	PPR repeat family
PPR	PF01535	197 - 226	4.4e-07	PPR repeat
PPR_3	PF13812	217 - 278	4.1e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	228 - 275	3.2e-10	PPR repeat family
PPR_long	PF17177	248 - 349	2.2e-11	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	265 - 312	2e-12	PPR repeat family
PPR_3	PF13812	267 - 310	3.3e-09	Pentatricopeptide repeat domain
PPR	PF01535	267 - 296	5.1e-06	PPR repeat
PPR_1	PF12854	296 - 327	4.8e-08	PPR repeat
PPR_2	PF13041	300 - 346	1e-09	PPR repeat family
PPR	PF01535	302 - 330	3.2e-07	PPR repeat
PPR_3	PF13812	322 - 381	4e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	333 - 380	6.1e-11	PPR repeat family
TPR_24	PF23276	347 - 459	5.3e-08	Fungal tetratrico peptide repeats
PPR_long	PF17177	354 - 468	1.3e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	364 - 397	6.7e-08	PPR repeat
PPR_2	PF13041	371 - 413	1.6e-07	PPR repeat family
PPR	PF01535	372 - 401	9.6e-08	PPR repeat
PPR_2	PF13041	474 - 515	2.8e-07	PPR repeat family
PPR_3	PF13812	497 - 555	8.1e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	508 - 556	1.1e-08	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35130 AT2G35130 AT2G35130
fragaria_vesca	FvH4_3g12360
malus_domestica	MD05G1251300.v1.1 MD10G1231700.v1.1 MD10G1231800.v1.1
prunus_persica	Prupe.4G112600_v2.0.a1
pyrus_communis	pycom05g20750 pycom05g22900 pycom10g19500 pycom10g19510
rosa_chinensis	RchiOBHm_Chr5g0020151
rosa_laevigata	RLG00000032505
rosa_roxburghii	Rroxscaffold_1G00058050
rosa_rugosa	Rorug05G0057400 Rorug05G0057500
rosa_samantha	Rh5AG147600 Rh5AG147700 Rh5BG147200 Rh5CG158600 Rh5DG146500
rosa_wichuraiana	Rw5G013050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1322
Acc36I	ACCTGC	1 cut(s)	1678
AccI	GTMKAC	3 cut(s)	803, 889, 908
AccII	CGCG	2 cut(s)	156, 1546
AciI	CCGC	6 cut(s)	156, 664, 729, 956, 1192, 1239
AclWI	GGATC	1 cut(s)	244
AcoI	YGGCCR	1 cut(s)	1351
AcsI	RAATTY	5 cut(s)	161, 336, 963, 1068, 1593
AcuI	CTGAAG	2 cut(s)	603, 663
AdeI	CACNNNGTG	1 cut(s)	686
AfaI	GTAC	5 cut(s)	455, 1013, 1429, 1650, 1696
AfiI	CCNNNNNNNGG	1 cut(s)	1634
AgsI	TTSAA	6 cut(s)	175, 469, 563, 1181, 1519, 1668
AhdI	GACNNNNNGTC	1 cut(s)	1545
AjnI	CCWGG	1 cut(s)	1643
AjuI	GAANNNNNNNTTGG	4 cut(s)	313, 345, 636, 668
Alw21I	GWGCWC	2 cut(s)	464, 1491
Alw26I	GTCTC	3 cut(s)	410, 726, 791
AlwI	GGATC	1 cut(s)	244
AlwNI	CAGNNNCTG	1 cut(s)	292
Ama87I	CYCGRG	1 cut(s)	1561
AoxI	GGCC	4 cut(s)	217, 609, 1351, 1360
ApeKI	GCWGC	4 cut(s)	1061, 1084, 1172, 1415
ApoI	RAATTY	5 cut(s)	161, 336, 963, 1068, 1593
ArsI	GACNNNNNNTTYG	2 cut(s)	1525, 1557
AspS9I	GGNCC	2 cut(s)	217, 1406
AsuC2I	CCSGG	1 cut(s)	1303
AsuII	TTCGAA	1 cut(s)	1599
AvaI	CYCGRG	1 cut(s)	1561
AvaII	GGWCC	1 cut(s)	1406
BanII	GRGCYC	3 cut(s)	275, 464, 1491
BauI	CACGAG	1 cut(s)	1579
BbsI	GAAGAC	3 cut(s)	591, 668, 1145
Bbv12I	GWGCWC	2 cut(s)	464, 1491
BbvI	GCAGC	4 cut(s)	1048, 1096, 1159, 1427
BccI	CCATC	6 cut(s)	13, 78, 219, 796, 1369, 1474
BciT130I	CCWGG	1 cut(s)	1645
BcnI	CCSGG	1 cut(s)	1303
BcoDI	GTCTC	3 cut(s)	410, 726, 791
BfaI	CTAG	2 cut(s)	192, 1313
BfmI	CTRYAG	1 cut(s)	1062
BfuAI	ACCTGC	1 cut(s)	1678
BglI	GCCNNNNNGGC	1 cut(s)	1359
BglII	AGATCT	1 cut(s)	753
BisI	GCNGC	4 cut(s)	1062, 1085, 1173, 1416
BlsI	GCNGC	4 cut(s)	1063, 1086, 1174, 1417
BmcAI	AGTACT	1 cut(s)	1696
Bme1390I	CCNGG	2 cut(s)	1303, 1645
Bme18I	GGWCC	1 cut(s)	1406
BmeRI	GACNNNNNGTC	1 cut(s)	1545
BmeT110I	CYCGRG	1 cut(s)	1561
BmgT120I	GGNCC	2 cut(s)	217, 1406
BmiI	GGNNCC	3 cut(s)	218, 424, 1408
BmrFI	CCNGG	2 cut(s)	1303, 1645
BpiI	GAAGAC	3 cut(s)	591, 668, 1145
Bpu14I	TTCGAA	1 cut(s)	1599
BpuMI	CCSGG	1 cut(s)	1303
BsaAI	YACGTR	1 cut(s)	906
BsaBI	GATNNNNATC	1 cut(s)	812
BsaI	GGTCTC	1 cut(s)	726
BsaJI	CCNNGG	4 cut(s)	220, 1302, 1644, 1654
BsaWI	WCCGGW	1 cut(s)	1522
BsaXI	ACNNNNNCTCC	4 cut(s)	446, 476, 1135, 1165
Bsc4I	CCNNNNNNNGG	1 cut(s)	1634
Bse118I	RCCGGY	2 cut(s)	1353, 1616
Bse1I	ACTGG	4 cut(s)	37, 102, 283, 1363
Bse3DI	GCAATG	1 cut(s)	1401
Bse8I	GATNNNNATC	1 cut(s)	812
BseBI	CCWGG	1 cut(s)	1645
BseDI	CCNNGG	4 cut(s)	220, 1302, 1644, 1654
BseGI	GGATG	4 cut(s)	230, 771, 1100, 1531
BseJI	GATNNNNATC	1 cut(s)	812
BseLI	CCNNNNNNNGG	1 cut(s)	1634
BseMI	GCAATG	1 cut(s)	1401
BseMII	CTCAG	1 cut(s)	786
BseNI	ACTGG	4 cut(s)	37, 102, 283, 1363
BseRI	GAGGAG	1 cut(s)	32
BseXI	GCAGC	4 cut(s)	1048, 1096, 1159, 1427
BsgI	GTGCAG	2 cut(s)	323, 1062
Bsh1236I	CGCG	2 cut(s)	156, 1546
Bsh1285I	CGRYCG	1 cut(s)	319
BshFI	GGCC	4 cut(s)	219, 611, 1353, 1362
BsiEI	CGRYCG	1 cut(s)	319
BsiHKAI	GWGCWC	2 cut(s)	464, 1491
BsiHKCI	CYCGRG	1 cut(s)	1561
BsiSI	CCGG	5 cut(s)	1302, 1354, 1523, 1617, 1635
BslFI	GGGAC	2 cut(s)	386, 950
BslI	CCNNNNNNNGG	1 cut(s)	1634
BsmAI	GTCTC	3 cut(s)	410, 726, 791
BsmBI	CGTCTC	2 cut(s)	410, 791
BsmFI	GGGAC	2 cut(s)	386, 950
BsnI	GGCC	4 cut(s)	219, 611, 1353, 1362
Bso31I	GGTCTC	1 cut(s)	726
BsoBI	CYCGRG	1 cut(s)	1561
Bsp119I	TTCGAA	1 cut(s)	1599
Bsp1286I	GDGCHC	3 cut(s)	275, 464, 1491
Bsp1407I	TGTACA	1 cut(s)	453
Bsp143I	GATC	5 cut(s)	236, 316, 753, 775, 1675
BspACI	CCGC	6 cut(s)	156, 664, 729, 956, 1192, 1239
BspANI	GGCC	4 cut(s)	219, 611, 1353, 1362
BspCNI	CTCAG	1 cut(s)	787
BspFNI	CGCG	2 cut(s)	156, 1546
BspHI	TCATGA	1 cut(s)	1156
BspLI	GGNNCC	3 cut(s)	218, 424, 1408
BspMAI	CTGCAG	1 cut(s)	1066
BspMI	ACCTGC	1 cut(s)	1678
BspPI	GGATC	1 cut(s)	244
BspQI	GCTCTTC	2 cut(s)	116, 1479
BspT104I	TTCGAA	1 cut(s)	1599
BspTNI	GGTCTC	1 cut(s)	726
BsrDI	GCAATG	1 cut(s)	1401
BsrFI	RCCGGY	2 cut(s)	1353, 1616
BsrGI	TGTACA	1 cut(s)	453
BsrI	ACTGG	4 cut(s)	37, 102, 283, 1363
BssAI	RCCGGY	2 cut(s)	1353, 1616
BssECI	CCNNGG	4 cut(s)	220, 1302, 1644, 1654
BssMI	GATC	5 cut(s)	236, 316, 753, 775, 1675
BssNAI	GTATAC	2 cut(s)	804, 909
BssSI	CACGAG	1 cut(s)	1579
BssT1I	CCWWGG	1 cut(s)	1654
Bst1107I	GTATAC	2 cut(s)	804, 909
Bst2BI	CACGAG	1 cut(s)	1579
Bst2UI	CCWGG	1 cut(s)	1645
Bst4CI	ACNGT	7 cut(s)	189, 326, 417, 1037, 1213, 1699, 1732
Bst6I	CTCTTC	5 cut(s)	116, 354, 764, 1176, 1479
BstAPI	GCANNNNNTGC	1 cut(s)	1172
BstAUI	TGTACA	1 cut(s)	453
BstBAI	YACGTR	1 cut(s)	906
BstBI	TTCGAA	1 cut(s)	1599
BstC8I	GCNNGC	3 cut(s)	785, 1255, 1712
BstDEI	CTNAG	2 cut(s)	212, 795
BstF5I	GGATG	4 cut(s)	230, 771, 1100, 1531
BstFNI	CGCG	2 cut(s)	156, 1546
BstKTI	GATC	5 cut(s)	239, 319, 756, 778, 1678
BstMAI	GTCTC	3 cut(s)	410, 726, 791
BstMBI	GATC	5 cut(s)	236, 316, 753, 775, 1675
BstMCI	CGRYCG	1 cut(s)	319
BstMWI	GCNNNNNNNGC	5 cut(s)	520, 1016, 1172, 1359, 1640
BstNI	CCWGG	1 cut(s)	1645
BstNSI	RCATGY	2 cut(s)	1339, 1714
BstSCI	CCNGG	2 cut(s)	1301, 1643
BstSFI	CTRYAG	1 cut(s)	1062
BstUI	CGCG	2 cut(s)	156, 1546
BstV1I	GCAGC	4 cut(s)	1048, 1096, 1159, 1427
BstV2I	GAAGAC	3 cut(s)	591, 668, 1145
BstX2I	RGATCY	1 cut(s)	753
BstYI	RGATCY	1 cut(s)	753
BstZ17I	GTATAC	2 cut(s)	804, 909
BsuRI	GGCC	4 cut(s)	219, 611, 1353, 1362
BtsCI	GGATG	4 cut(s)	230, 771, 1100, 1531
BtsIMutI	CAGTG	3 cut(s)	276, 422, 1218
BveI	ACCTGC	1 cut(s)	1678
Cac8I	GCNNGC	3 cut(s)	785, 1255, 1712
CaiI	CAGNNNCTG	1 cut(s)	292
CciI	TCATGA	1 cut(s)	1156
Cfr10I	RCCGGY	2 cut(s)	1353, 1616
Cfr13I	GGNCC	2 cut(s)	217, 1406
CseI	GACGC	2 cut(s)	512, 1535
Csp6I	GTAC	5 cut(s)	454, 1012, 1428, 1649, 1695
CviAII	CATG	6 cut(s)	1090, 1126, 1157, 1336, 1536, 1711
CviQI	GTAC	5 cut(s)	454, 1012, 1428, 1649, 1695
DdeI	CTNAG	2 cut(s)	212, 795
DpnI	GATC	5 cut(s)	238, 318, 755, 777, 1677
DpnII	GATC	5 cut(s)	236, 316, 753, 775, 1675
DraI	TTTAAA	1 cut(s)	606
DraIII	CACNNNGTG	1 cut(s)	686
DrdI	GACNNNNNNGTC	1 cut(s)	1322
DriI	GACNNNNNGTC	1 cut(s)	1545
DseDI	GACNNNNNNGTC	1 cut(s)	1322
EaeI	YGGCCR	1 cut(s)	1351
Eam1104I	CTCTTC	5 cut(s)	116, 354, 764, 1176, 1479
Eam1105I	GACNNNNNGTC	1 cut(s)	1545
EarI	CTCTTC	5 cut(s)	116, 354, 764, 1176, 1479
EciI	GGCGGA	2 cut(s)	744, 1228
Ecl136II	GAGCTC	2 cut(s)	462, 1489
Eco130I	CCWWGG	1 cut(s)	1654
Eco147I	AGGCCT	1 cut(s)	611
Eco24I	GRGCYC	3 cut(s)	275, 464, 1491
Eco31I	GGTCTC	1 cut(s)	726
Eco32I	GATATC	2 cut(s)	379, 1423
Eco47I	GGWCC	1 cut(s)	1406
Eco53kI	GAGCTC	2 cut(s)	462, 1489
Eco57I	CTGAAG	2 cut(s)	603, 663
Eco88I	CYCGRG	1 cut(s)	1561
EcoICRI	GAGCTC	2 cut(s)	462, 1489
EcoO109I	RGGNCCY	1 cut(s)	1406
EcoRII	CCWGG	1 cut(s)	1643
EcoRV	GATATC	2 cut(s)	379, 1423
EcoT14I	CCWWGG	1 cut(s)	1654
EcoT22I	ATGCAT	3 cut(s)	575, 928, 1138
EcoT38I	GRGCYC	3 cut(s)	275, 464, 1491
ErhI	CCWWGG	1 cut(s)	1654
Esp3I	CGTCTC	2 cut(s)	410, 791
FaeI	CATG	6 cut(s)	1093, 1129, 1160, 1339, 1539, 1714
FalI	AAGNNNNNCTT	4 cut(s)	449, 481, 826, 858
FaqI	GGGAC	2 cut(s)	386, 950
FatI	CATG	6 cut(s)	1089, 1125, 1156, 1335, 1535, 1710
FauNDI	CATATG	4 cut(s)	368, 899, 1138, 1224
FblI	GTMKAC	3 cut(s)	803, 889, 908
Fnu4HI	GCNGC	4 cut(s)	1062, 1085, 1173, 1416
FokI	GGATG	4 cut(s)	237, 778, 1107, 1538
FriOI	GRGCYC	3 cut(s)	275, 464, 1491
Fsp4HI	GCNGC	4 cut(s)	1062, 1085, 1173, 1416
FspBI	CTAG	2 cut(s)	192, 1313
GluI	GCNGC	4 cut(s)	1062, 1085, 1173, 1416
HaeIII	GGCC	4 cut(s)	219, 611, 1353, 1362
HapII	CCGG	5 cut(s)	1302, 1354, 1523, 1617, 1635
HgaI	GACGC	2 cut(s)	512, 1535
Hin1II	CATG	6 cut(s)	1093, 1129, 1160, 1339, 1539, 1714
HindIII	AAGCTT	2 cut(s)	169, 1029
HinfI	GANTC	3 cut(s)	289, 539, 1298
HpaII	CCGG	5 cut(s)	1302, 1354, 1523, 1617, 1635
Hpy166II	GTNNAC	6 cut(s)	250, 804, 890, 909, 1100, 1285
Hpy188I	TCNGA	3 cut(s)	167, 762, 1745
Hpy8I	GTNNAC	6 cut(s)	250, 804, 890, 909, 1100, 1285
Hpy99I	CGWCG	3 cut(s)	137, 506, 1676
HpyAV	CCTTC	4 cut(s)	56, 301, 475, 608
HpyCH4III	ACNGT	7 cut(s)	189, 326, 417, 1037, 1213, 1699, 1732
HpyCH4IV	ACGT	4 cut(s)	387, 800, 905, 1260
HpyF10VI	GCNNNNNNNGC	5 cut(s)	520, 1016, 1172, 1359, 1640
HpyF3I	CTNAG	2 cut(s)	212, 795
HpySE526I	ACGT	4 cut(s)	387, 800, 905, 1260
Hsp92II	CATG	6 cut(s)	1093, 1129, 1160, 1339, 1539, 1714
Kzo9I	GATC	5 cut(s)	236, 316, 753, 775, 1675
LguI	GCTCTTC	2 cut(s)	116, 1479
LmnI	GCTCC	7 cut(s)	45, 467, 983, 1058, 1309, 1553, 1658
Lsp1109I	GCAGC	4 cut(s)	1048, 1096, 1159, 1427
MaeI	CTAG	2 cut(s)	192, 1313
MaeII	ACGT	4 cut(s)	387, 800, 905, 1260
MaeIII	GTNAC	2 cut(s)	717, 1531
MalI	GATC	5 cut(s)	238, 318, 755, 777, 1677
MboI	GATC	5 cut(s)	236, 316, 753, 775, 1675
MfeI	CAATTG	1 cut(s)	690
MflI	RGATCY	1 cut(s)	753
MhlI	GDGCHC	3 cut(s)	275, 464, 1491
MluCI	AATT	9 cut(s)	56, 161, 336, 490, 690, 817, 963, 1068, 1593
MlyI	GAGTC	1 cut(s)	1307
MnlI	CCTC	9 cut(s)	53, 215, 367, 747, 756, 927, 1267, 1372, 1714
Mph1103I	ATGCAT	3 cut(s)	575, 928, 1138
MseI	TTAA	5 cut(s)	384, 605, 1329, 1340, 1446
MslI	CAYNNNNRTG	3 cut(s)	75, 531, 1161
MspA1I	CMGCKG	1 cut(s)	1418
MspI	CCGG	5 cut(s)	1302, 1354, 1523, 1617, 1635
MspR9I	CCNGG	2 cut(s)	1303, 1645
MunI	CAATTG	1 cut(s)	690
MvaI	CCWGG	1 cut(s)	1645
MvnI	CGCG	2 cut(s)	156, 1546
MwoI	GCNNNNNNNGC	5 cut(s)	520, 1016, 1172, 1359, 1640
NciI	CCSGG	1 cut(s)	1303
NdeI	CATATG	4 cut(s)	368, 899, 1138, 1224
NdeII	GATC	5 cut(s)	236, 316, 753, 775, 1675
NlaIII	CATG	6 cut(s)	1093, 1129, 1160, 1339, 1539, 1714
NlaIV	GGNNCC	3 cut(s)	218, 424, 1408
NmeAIII	GCCGAG	1 cut(s)	245
NmuCI	GTSAC	1 cut(s)	1531
NsiI	ATGCAT	3 cut(s)	575, 928, 1138
NspI	RCATGY	2 cut(s)	1339, 1714
NspV	TTCGAA	1 cut(s)	1599
PaeI	GCATGC	1 cut(s)	1714
PaeR7I	CTCGAG	1 cut(s)	1561
PagI	TCATGA	1 cut(s)	1156
PceI	AGGCCT	1 cut(s)	611
PciSI	GCTCTTC	2 cut(s)	116, 1479
PcsI	WCGNNNNNNNCGW	1 cut(s)	1680
PfeI	GAWTC	2 cut(s)	289, 539
PkrI	GCNGC	4 cut(s)	1063, 1086, 1174, 1417
Ple19I	CGATCG	1 cut(s)	319
PleI	GAGTC	1 cut(s)	1306
PpsI	GAGTC	1 cut(s)	1306
Ppu21I	YACGTR	1 cut(s)	906
PpuMI	RGGWCCY	1 cut(s)	1406
Psp124BI	GAGCTC	2 cut(s)	464, 1491
Psp5II	RGGWCCY	1 cut(s)	1406
Psp6I	CCWGG	1 cut(s)	1643
PspGI	CCWGG	1 cut(s)	1643
PspN4I	GGNNCC	3 cut(s)	218, 424, 1408
PspPI	GGNCC	2 cut(s)	217, 1406
PspPPI	RGGWCCY	1 cut(s)	1406
PstI	CTGCAG	1 cut(s)	1066
PstNI	CAGNNNCTG	1 cut(s)	292
PsuI	RGATCY	1 cut(s)	753
PvuI	CGATCG	1 cut(s)	319
PvuII	CAGCTG	1 cut(s)	1418
RsaI	GTAC	5 cut(s)	455, 1013, 1429, 1650, 1696
RsaNI	GTAC	5 cut(s)	454, 1012, 1428, 1649, 1695
RseI	CAYNNNNRTG	3 cut(s)	75, 531, 1161
SacI	GAGCTC	2 cut(s)	464, 1491
SapI	GCTCTTC	2 cut(s)	116, 1479
SaqAI	TTAA	5 cut(s)	384, 605, 1329, 1340, 1446
SatI	GCNGC	4 cut(s)	1062, 1085, 1173, 1416
Sau3AI	GATC	5 cut(s)	236, 316, 753, 775, 1675
Sau96I	GGNCC	2 cut(s)	217, 1406
ScaI	AGTACT	1 cut(s)	1696
SchI	GAGTC	1 cut(s)	1307
ScrFI	CCNGG	2 cut(s)	1303, 1645
SduI	GDGCHC	3 cut(s)	275, 464, 1491
SfcI	CTRYAG	1 cut(s)	1062
SfiI	GGCCNNNNNGGCC	1 cut(s)	1359
Sfr274I	CTCGAG	1 cut(s)	1561
SfuI	TTCGAA	1 cut(s)	1599
SinI	GGWCC	1 cut(s)	1406
SlaI	CTCGAG	1 cut(s)	1561
SmiMI	CAYNNNNRTG	3 cut(s)	75, 531, 1161
SmlI	CTYRAG	1 cut(s)	1561
SmoI	CTYRAG	1 cut(s)	1561
SphI	GCATGC	1 cut(s)	1714
Sse9I	AATT	9 cut(s)	56, 161, 336, 490, 690, 817, 963, 1068, 1593
SseBI	AGGCCT	1 cut(s)	611
SsiI	CCGC	6 cut(s)	156, 664, 729, 956, 1192, 1239
SspMI	CTAG	2 cut(s)	192, 1313
SstI	GAGCTC	2 cut(s)	464, 1491
StuI	AGGCCT	1 cut(s)	611
StyD4I	CCNGG	2 cut(s)	1301, 1643
StyI	CCWWGG	1 cut(s)	1654
TaaI	ACNGT	7 cut(s)	189, 326, 417, 1037, 1213, 1699, 1732
TaiI	ACGT	4 cut(s)	390, 803, 908, 1263
TasI	AATT	9 cut(s)	56, 161, 336, 490, 690, 817, 963, 1068, 1593
TatI	WGTACW	3 cut(s)	453, 1427, 1694
TfiI	GAWTC	2 cut(s)	289, 539
Tru1I	TTAA	5 cut(s)	384, 605, 1329, 1340, 1446
Tru9I	TTAA	5 cut(s)	384, 605, 1329, 1340, 1446
TscAI	CASTG	3 cut(s)	283, 422, 1218
TseFI	GTSAC	1 cut(s)	1531
TseI	GCWGC	4 cut(s)	1061, 1084, 1172, 1415
Tsp45I	GTSAC	1 cut(s)	1531
TspDTI	ATGAA	6 cut(s)	59, 782, 887, 1145, 1173, 1202
TspGWI	ACGGA	5 cut(s)	150, 267, 840, 954, 1071
TspRI	CASTG	3 cut(s)	283, 422, 1218
VpaK11BI	GGWCC	1 cut(s)	1406
XapI	RAATTY	5 cut(s)	161, 336, 963, 1068, 1593
XceI	RCATGY	2 cut(s)	1339, 1714
XhoI	CTCGAG	1 cut(s)	1561
XmiI	GTMKAC	3 cut(s)	803, 889, 908
XspI	CTAG	2 cut(s)	192, 1313
ZrmI	AGTACT	1 cut(s)	1696
Zsp2I	ATGCAT	3 cut(s)	575, 928, 1138

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.