Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0008257

Physical Location & Seq

Genomic Coordinates Forward (+)

79970358 .. 79973153

Gene Structure 2796 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00058050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGTTCAAAATTGCGGACGGTCTTCTTTATATAGGTGGTTCTCTAAGGAGCCGAGGATGGAAGTATGGATCCGGGTTTGTTGATGGAATTTTCCCAGTGATGAGCCCCACTGCCGAGCAGCTTCTGGACATCGTACAGAACAAAGCAGATAAAATTAAAATTTGGGAAGTTCTTGACACTCTTCCGGCCTCTCATATTATATGGGATGACATCGTTAATGTAGCTGTTCAACTTCGTCTCAATAAAATGTGGGATTCCATAATCTTGATATGTGAGTGGATATTGCACAAGAGCTCCTACAAGCCAGATGTAATCTGCTACAATTTACTGCTAGATGCTTATGGGCAGAAATCACTGCATAAAGAGGCAGAATCCACATATTTGGAACTTCTTGAAGCTCGATGCATTCCCACCGAGGACACTTATGCCCTTCTTATAAAGGCCTACTGCAAGTCTGGATTGCTAGATAGGGCTGAAGCCATTTTTGGTGAAATGCGGAAGTATGGTCTTCCTCCAAGTGCAATTGTGTTCGATGCTTATATTAATGGGTTGATAAAAGGAGGAAACCCTCAAAAAGCAGAAGAGATCTTTCAGAGGATGAAAAGAGACCAATGCGAACCATCTGCCGACACTTATACTATGTTAATCAATATATATGGGAAGGCAAGAAAATCCTATATGGCCCTGAAACTGTTCCGCGAAATGAGAAGTCAGAAATGTAAACCGAACATATGCACATATACTGCTTTGATAAATGCATTTGCTAGGGAAGGAAATTGTGAGAAAGCAGAAGAAATATTTGAGCAGCTACAAGAGGCAGGGATTGAGCCTGATGTGTATACTTACAATGCCCTTATGGAAGCTTACAGTCGAGCAGGATATCCTTATGGAGCTGCAGAAATTTTCTCTTTAATGCAGCACATGGGATGTGAACCAGATAGAGCATCATATAATATCATGGTAGATGCATATGGTAGAGGTGGTCTGCATGAAGATGCGCAAGCTGCGTTTGAAGAGATGAAGCGATTAGGAATAACCCCAACAATAAAATCCCACATGCTACTTTTATCTGCCTACTCCAAAGCTGGCAATGTAGCTAGATGTGAAGACATTGTGAACCAGATGCAAGAATCCGGGCTGGAGCTAGACACATTTGTGATTAACAGTATGCTAAACCTGTATGGCCGGCTAGGTCAGCTAGAAAAAATGGAGGAAGTTTTGACAGCACTGGAAAAAGGACCGTATGCAGCTGATATCAGTACATATAACATCTTGATTAACATTTATGGAAGGGCAGGATTTTTCGACAAGATGGAAGAGCTCTTCCAATCACTTCCAGCAAAGAAATTAAAACCTGATGTTGTGACATGGACTTCTCGTCTTGGAGCATACTCTAGGAAGAAACTATACACAAGATGCTTGGAAATCTTTGAAGAAATGATTGATGCGGGTTGCTATCCAGATGGCGGAACTGCCAAAGTACTTCTCGCTTCCTGCTCAAGCGAAGATCAAACCGAGCAGTTTTACACTTTCTGTAATTATGTTGATTTCCAAGAAACTGAAGAAGGCATTCACACTAGTGCAAAGAAGGATCCAAATTCTTATAATAAAGTATTGGAATTTTACACTGACATGATCCAGATTTGCAATGTAGAAAAGGGTATAAGAAAAACCAAAATACCACAAGTGGGTCAAAATGAAAAACACAAAATGTCGGTCCACTCTCTAGTGCTAAAGATACCCATGGTATTATGGAAATTCCATGCAACCTTTGAACCACCTCGGCGACGTCTCGATTTAGGATCCAACACTGTCCACCTCACTCTGCCACTCACCAACTTCAGCTTTTCAGTGCTTATTGGGTTTGTTGACAAAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

626

Amino Acids

71.01

Weight (kDa)

5.81

Isoelectric Point (pI)

37.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	102 - 151	1.6e-09	PPR repeat family
PPR_1	PF12854	135 - 166	6.2e-08	PPR repeat
PPR	PF01535	141 - 170	3.9e-07	PPR repeat
PPR_2	PF13041	141 - 186	1.4e-07	PPR repeat family
PPR_3	PF13812	161 - 221	3e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	172 - 218	7.4e-10	PPR repeat family
PPR_long	PF17177	192 - 293	9.2e-13	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	211 - 256	1.2e-12	PPR repeat family
PPR	PF01535	211 - 240	5.8e-06	PPR repeat
PPR_3	PF13812	222 - 254	8.8e-07	Pentatricopeptide repeat domain
PPR_3	PF13812	230 - 289	6.7e-17	Pentatricopeptide repeat domain
PPR_1	PF12854	240 - 269	3.1e-08	PPR repeat
PPR_2	PF13041	242 - 290	1.7e-14	PPR repeat family
PPR	PF01535	246 - 275	9.3e-08	PPR repeat
TPR_24	PF23276	251 - 322	5.8e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	266 - 325	3.8e-13	Pentatricopeptide repeat domain
PPR_2	PF13041	279 - 324	8.7e-11	PPR repeat family
TPR_24	PF23276	291 - 403	5.3e-07	Fungal tetratrico peptide repeats
PPR_long	PF17177	298 - 413	2.9e-08	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	308 - 341	8.3e-07	PPR repeat
PPR_2	PF13041	315 - 351	6.1e-06	PPR repeat family
PPR	PF01535	316 - 345	4.1e-07	PPR repeat
PPR_2	PF13041	418 - 459	3.7e-07	PPR repeat family
PPR_2	PF13041	452 - 499	5e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008257)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35130 AT2G35130 AT2G35130
fragaria_vesca	FvH4_3g12360
malus_domestica	MD05G1251300.v1.1 MD10G1231700.v1.1 MD10G1231800.v1.1
prunus_persica	Prupe.4G112600_v2.0.a1
pyrus_communis	pycom05g20750 pycom05g22900 pycom10g19500 pycom10g19510
rosa_chinensis	RchiOBHm_Chr5g0020151
rosa_laevigata	RLG00000032505
rosa_roxburghii	Rroxscaffold_1G00058050
rosa_rugosa	Rorug05G0057400 Rorug05G0057500
rosa_samantha	Rh5AG147600 Rh5AG147700 Rh5BG147200 Rh5CG158600 Rh5DG146500
rosa_wichuraiana	Rw5G013050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	437, 1605
AatII	GACGTC	1 cut(s)	1792
Acc16I	TGCGCA	1 cut(s)	999
AccI	GTMKAC	1 cut(s)	839
AccII	CGCG	1 cut(s)	699
AciI	CCGC	5 cut(s)	14, 496, 697, 1448, 1467
AclWI	GGATC	7 cut(s)	63, 76, 1586, 1599, 1630, 1797, 1810
AcoI	YGGCCR	1 cut(s)	1183
AcsI	RAATTY	6 cut(s)	87, 159, 900, 1597, 1619, 1757
AcuI	CTGAAG	3 cut(s)	495, 1581, 1825
AcyI	GRCGYC	1 cut(s)	1789
AfaI	GTAC	3 cut(s)	135, 1261, 1482
AfiI	CCNNNNNNNGG	2 cut(s)	1466, 1688
AgsI	TTSAA	6 cut(s)	7, 230, 395, 1013, 1433, 1775
AhdI	GACNNNNNGTC	1 cut(s)	1377
AhlI	ACTAGT	1 cut(s)	1577
AjuI	GAANNNNNNNTTGG	2 cut(s)	468, 500
AleI	CACNNNNGTG	2 cut(s)	1154, 1578
Alw21I	GWGCWC	2 cut(s)	296, 1323
Alw26I	GTCTC	3 cut(s)	242, 600, 1796
AlwI	GGATC	7 cut(s)	63, 76, 1586, 1599, 1630, 1797, 1810
AlwNI	CAGNNNCTG	1 cut(s)	124
AoxI	GGCC	4 cut(s)	186, 441, 681, 1183
ApeKI	GCWGC	6 cut(s)	118, 805, 893, 916, 1004, 1247
ApoI	RAATTY	6 cut(s)	87, 159, 900, 1597, 1619, 1757
ArsI	GACNNNNNNTTYG	2 cut(s)	1701, 1733
AseI	ATTAAT	1 cut(s)	543
Asp700I	GAANNNNTTC	2 cut(s)	692, 1569
AspLEI	GCGC	1 cut(s)	1000
AspS9I	GGNCC	3 cut(s)	682, 1238, 1717
AsuC2I	CCSGG	2 cut(s)	73, 1135
AsuHPI	GGTGA	2 cut(s)	500, 1825
AvaII	GGWCC	2 cut(s)	1238, 1717
BamHI	GGATCC	3 cut(s)	68, 1591, 1802
BanII	GRGCYC	3 cut(s)	107, 296, 1323
BarI	GAAGNNNNNNTAC	2 cut(s)	1391, 1423
BbsI	GAAGAC	3 cut(s)	14, 500, 1113
Bbv12I	GWGCWC	2 cut(s)	296, 1323
BbvI	GCAGC	6 cut(s)	130, 817, 880, 928, 991, 1259
BccI	CCATC	5 cut(s)	51, 77, 628, 1306, 1457
BcgI	CGANNNNNNTGC	2 cut(s)	605, 639
BcnI	CCSGG	2 cut(s)	73, 1135
BcoDI	GTCTC	3 cut(s)	242, 600, 1796
BcuI	ACTAGT	1 cut(s)	1577
BfmI	CTRYAG	1 cut(s)	894
BglII	AGATCT	1 cut(s)	585
BisI	GCNGC	6 cut(s)	119, 806, 894, 917, 1005, 1248
BlsI	GCNGC	6 cut(s)	120, 807, 895, 918, 1006, 1249
BmcAI	AGTACT	1 cut(s)	1482
Bme1390I	CCNGG	2 cut(s)	73, 1135
Bme18I	GGWCC	2 cut(s)	1238, 1717
BmeRI	GACNNNNNGTC	1 cut(s)	1377
BmgT120I	GGNCC	3 cut(s)	682, 1238, 1717
BmiI	GGNNCC	4 cut(s)	50, 70, 1593, 1804
BmrFI	CCNGG	2 cut(s)	73, 1135
BmrI	ACTGGG	1 cut(s)	89
BmsI	GCATC	9 cut(s)	325, 392, 523, 953, 955, 985, 1113, 1406, 1435
BmuI	ACTGGG	1 cut(s)	89
BpiI	GAAGAC	3 cut(s)	14, 500, 1113
BpmI	CTGGAG	1 cut(s)	1160
BpuEI	CTTGAG	1 cut(s)	1483
BpuMI	CCSGG	2 cut(s)	73, 1135
BsaHI	GRCGYC	1 cut(s)	1789
BsaI	GGTCTC	1 cut(s)	600
BsaJI	CCNNGG	4 cut(s)	52, 414, 1743, 1781
BsaXI	ACNNNNNCTCC	2 cut(s)	278, 308
Bsc4I	CCNNNNNNNGG	2 cut(s)	1466, 1688
Bse118I	RCCGGY	1 cut(s)	1185
Bse1I	ACTGG	2 cut(s)	95, 1233
Bse3DI	GCAATG	2 cut(s)	1096, 1654
BseDI	CCNNGG	4 cut(s)	52, 414, 1743, 1781
BseGI	GGATG	4 cut(s)	62, 211, 603, 932
BseLI	CCNNNNNNNGG	2 cut(s)	1466, 1688
BseMI	GCAATG	2 cut(s)	1096, 1654
BseNI	ACTGG	2 cut(s)	95, 1233
BseXI	GCAGC	6 cut(s)	130, 817, 880, 928, 991, 1259
Bsh1236I	CGCG	1 cut(s)	699
BshFI	GGCC	4 cut(s)	188, 443, 683, 1185
BsiHKAI	GWGCWC	2 cut(s)	296, 1323
BsiSI	CCGG	4 cut(s)	72, 185, 1134, 1186
BslI	CCNNNNNNNGG	2 cut(s)	1466, 1688
BsmAI	GTCTC	3 cut(s)	242, 600, 1796
BsmBI	CGTCTC	2 cut(s)	242, 1796
BsmI	GAATGC	2 cut(s)	405, 1569
BsnI	GGCC	4 cut(s)	188, 443, 683, 1185
Bso31I	GGTCTC	1 cut(s)	600
Bsp1286I	GDGCHC	3 cut(s)	107, 296, 1323
Bsp143I	GATC	6 cut(s)	68, 585, 1507, 1591, 1635, 1802
Bsp19I	CCATGG	1 cut(s)	1743
BspACI	CCGC	5 cut(s)	14, 496, 697, 1448, 1467
BspANI	GGCC	4 cut(s)	188, 443, 683, 1185
BspFNI	CGCG	1 cut(s)	699
BspLI	GGNNCC	4 cut(s)	50, 70, 1593, 1804
BspMAI	CTGCAG	1 cut(s)	898
BspPI	GGATC	7 cut(s)	63, 76, 1586, 1599, 1630, 1797, 1810
BspQI	GCTCTTC	2 cut(s)	1311, 1328
BspTNI	GGTCTC	1 cut(s)	600
BsrDI	GCAATG	2 cut(s)	1096, 1654
BsrFI	RCCGGY	1 cut(s)	1185
BsrI	ACTGG	2 cut(s)	95, 1233
BssAI	RCCGGY	1 cut(s)	1185
BssECI	CCNNGG	4 cut(s)	52, 414, 1743, 1781
BssMI	GATC	6 cut(s)	68, 585, 1507, 1591, 1635, 1802
BssNAI	GTATAC	1 cut(s)	840
BssNI	GRCGYC	1 cut(s)	1789
BssT1I	CCWWGG	1 cut(s)	1743
Bst1107I	GTATAC	1 cut(s)	840
Bst4CI	ACNGT	6 cut(s)	20, 693, 869, 1166, 1242, 1813
Bst6I	CTCTTC	5 cut(s)	186, 576, 1008, 1311, 1328
BstACI	GRCGYC	1 cut(s)	1789
BstC8I	GCNNGC	3 cut(s)	1002, 1087, 1187
BstDEI	CTNAG	1 cut(s)	44
BstDSI	CCRYGG	1 cut(s)	1743
BstF5I	GGATG	4 cut(s)	62, 211, 603, 932
BstFNI	CGCG	1 cut(s)	699
BstHHI	GCGC	1 cut(s)	1000
BstKTI	GATC	6 cut(s)	71, 588, 1510, 1594, 1638, 1805
BstMAI	GTCTC	3 cut(s)	242, 600, 1796
BstMBI	GATC	6 cut(s)	68, 585, 1507, 1591, 1635, 1802
BstMWI	GCNNNNNNNGC	2 cut(s)	1004, 1195
BstNSI	RCATGY	1 cut(s)	1060
BstSCI	CCNGG	2 cut(s)	71, 1133
BstSFI	CTRYAG	1 cut(s)	894
BstUI	CGCG	1 cut(s)	699
BstV1I	GCAGC	6 cut(s)	130, 817, 880, 928, 991, 1259
BstV2I	GAAGAC	3 cut(s)	14, 500, 1113
BstX2I	RGATCY	4 cut(s)	68, 585, 1591, 1802
BstXI	CCANNNNNNTGG	1 cut(s)	382
BstYI	RGATCY	4 cut(s)	68, 585, 1591, 1802
BstZ17I	GTATAC	1 cut(s)	840
BsuRI	GGCC	4 cut(s)	188, 443, 683, 1185
BtgI	CCRYGG	1 cut(s)	1743
BtsCI	GGATG	4 cut(s)	62, 211, 603, 932
BtsI	GCAGTG	2 cut(s)	108, 353
BtsIMutI	CAGTG	7 cut(s)	102, 108, 353, 1226, 1626, 1809, 1857
Cac8I	GCNNGC	3 cut(s)	1002, 1087, 1187
CaiI	CAGNNNCTG	1 cut(s)	124
CfoI	GCGC	1 cut(s)	1000
Cfr10I	RCCGGY	1 cut(s)	1185
Cfr13I	GGNCC	3 cut(s)	682, 1238, 1717
Csp6I	GTAC	3 cut(s)	134, 1260, 1481
CviAII	CATG	8 cut(s)	922, 958, 989, 1057, 1368, 1633, 1744, 1763
CviQI	GTAC	3 cut(s)	134, 1260, 1481
DdeI	CTNAG	1 cut(s)	44
DpnI	GATC	6 cut(s)	70, 587, 1509, 1593, 1637, 1804
DpnII	GATC	6 cut(s)	68, 585, 1507, 1591, 1635, 1802
DriI	GACNNNNNGTC	1 cut(s)	1377
EaeI	YGGCCR	1 cut(s)	1183
Eam1104I	CTCTTC	5 cut(s)	186, 576, 1008, 1311, 1328
Eam1105I	GACNNNNNGTC	1 cut(s)	1377
EarI	CTCTTC	5 cut(s)	186, 576, 1008, 1311, 1328
EciI	GGCGGA	1 cut(s)	1482
Ecl136II	GAGCTC	2 cut(s)	294, 1321
Eco130I	CCWWGG	1 cut(s)	1743
Eco147I	AGGCCT	1 cut(s)	443
Eco24I	GRGCYC	3 cut(s)	107, 296, 1323
Eco31I	GGTCTC	1 cut(s)	600
Eco32I	GATATC	2 cut(s)	881, 1255
Eco47I	GGWCC	2 cut(s)	1238, 1717
Eco53kI	GAGCTC	2 cut(s)	294, 1321
Eco57I	CTGAAG	3 cut(s)	495, 1581, 1825
EcoICRI	GAGCTC	2 cut(s)	294, 1321
EcoRV	GATATC	2 cut(s)	881, 1255
EcoT14I	CCWWGG	1 cut(s)	1743
EcoT22I	ATGCAT	3 cut(s)	407, 760, 970
EcoT38I	GRGCYC	3 cut(s)	107, 296, 1323
ErhI	CCWWGG	1 cut(s)	1743
Esp3I	CGTCTC	2 cut(s)	242, 1796
FaeI	CATG	8 cut(s)	925, 961, 992, 1060, 1371, 1636, 1747, 1766
FatI	CATG	8 cut(s)	921, 957, 988, 1056, 1367, 1632, 1743, 1762
FauI	CCCGC	1 cut(s)	1441
FauNDI	CATATG	2 cut(s)	731, 970
FblI	GTMKAC	1 cut(s)	839
Fnu4HI	GCNGC	6 cut(s)	119, 806, 894, 917, 1005, 1248
FokI	GGATG	4 cut(s)	69, 218, 610, 939
FriOI	GRGCYC	3 cut(s)	107, 296, 1323
Fsp4HI	GCNGC	6 cut(s)	119, 806, 894, 917, 1005, 1248
FspI	TGCGCA	1 cut(s)	999
GlaI	GCGC	1 cut(s)	999
GluI	GCNGC	6 cut(s)	119, 806, 894, 917, 1005, 1248
GsuI	CTGGAG	1 cut(s)	1160
HaeIII	GGCC	4 cut(s)	188, 443, 683, 1185
HapII	CCGG	4 cut(s)	72, 185, 1134, 1186
HhaI	GCGC	1 cut(s)	1000
Hin1I	GRCGYC	1 cut(s)	1789
Hin1II	CATG	8 cut(s)	925, 961, 992, 1060, 1371, 1636, 1747, 1766
Hin6I	GCGC	1 cut(s)	998
HinP1I	GCGC	1 cut(s)	998
HincII	GTYRAC	1 cut(s)	1870
HindII	GTYRAC	1 cut(s)	1870
HindIII	AAGCTT	1 cut(s)	861
HinfI	GANTC	3 cut(s)	254, 371, 1130
HpaII	CCGG	4 cut(s)	72, 185, 1134, 1186
HphI	GGTGA	2 cut(s)	500, 1825
Hpy166II	GTNNAC	7 cut(s)	722, 840, 932, 1117, 1720, 1816, 1870
Hpy188I	TCNGA	2 cut(s)	594, 714
Hpy188III	TCNNGA	9 cut(s)	125, 173, 265, 392, 456, 1273, 1460, 1639, 1793
Hpy8I	GTNNAC	7 cut(s)	722, 840, 932, 1117, 1720, 1816, 1870
Hpy99I	CGWCG	1 cut(s)	1791
HpyAV	CCTTC	6 cut(s)	440, 655, 764, 1284, 1559, 1582
HpyCH4III	ACNGT	6 cut(s)	20, 693, 869, 1166, 1242, 1813
HpyCH4IV	ACGT	1 cut(s)	1789
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1004, 1195
HpyF3I	CTNAG	1 cut(s)	44
HpySE526I	ACGT	1 cut(s)	1789
Hsp92I	GRCGYC	1 cut(s)	1789
Hsp92II	CATG	8 cut(s)	925, 961, 992, 1060, 1371, 1636, 1747, 1766
HspAI	GCGC	1 cut(s)	998
KroI	GCCGGC	1 cut(s)	1185
KroNI	GCCGGC	1 cut(s)	1187
Kzo9I	GATC	6 cut(s)	68, 585, 1507, 1591, 1635, 1802
LguI	GCTCTTC	2 cut(s)	1311, 1328
LmnI	GCTCC	5 cut(s)	48, 299, 890, 1141, 1385
Lsp1109I	GCAGC	6 cut(s)	130, 817, 880, 928, 991, 1259
LweI	GCATC	9 cut(s)	325, 392, 523, 953, 955, 985, 1113, 1406, 1435
MaeII	ACGT	1 cut(s)	1789
MaeIII	GTNAC	1 cut(s)	1363
MalI	GATC	6 cut(s)	70, 587, 1509, 1593, 1637, 1804
MboI	GATC	6 cut(s)	68, 585, 1507, 1591, 1635, 1802
MfeI	CAATTG	1 cut(s)	522
MflI	RGATCY	4 cut(s)	68, 585, 1591, 1802
MhlI	GDGCHC	3 cut(s)	107, 296, 1323
MmeI	TCCRAC	1 cut(s)	1830
Mph1103I	ATGCAT	3 cut(s)	407, 760, 970
MroNI	GCCGGC	1 cut(s)	1185
MroXI	GAANNNNTTC	2 cut(s)	692, 1569
MseI	TTAA	8 cut(s)	156, 216, 543, 644, 911, 1161, 1278, 1349
MslI	CAYNNNNRTG	4 cut(s)	993, 1154, 1578, 1631
MspA1I	CMGCKG	1 cut(s)	1250
MspI	CCGG	4 cut(s)	72, 185, 1134, 1186
MspR9I	CCNGG	2 cut(s)	73, 1135
MunI	CAATTG	1 cut(s)	522
Mva1269I	GAATGC	2 cut(s)	405, 1569
MvnI	CGCG	1 cut(s)	699
MwoI	GCNNNNNNNGC	2 cut(s)	1004, 1195
NaeI	GCCGGC	1 cut(s)	1187
NciI	CCSGG	2 cut(s)	73, 1135
NcoI	CCATGG	1 cut(s)	1743
NdeI	CATATG	2 cut(s)	731, 970
NdeII	GATC	6 cut(s)	68, 585, 1507, 1591, 1635, 1802
NgoMIV	GCCGGC	1 cut(s)	1185
NlaIII	CATG	8 cut(s)	925, 961, 992, 1060, 1371, 1636, 1747, 1766
NlaIV	GGNNCC	4 cut(s)	50, 70, 1593, 1804
NmeAIII	GCCGAG	3 cut(s)	77, 139, 1762
NmuCI	GTSAC	1 cut(s)	1363
NsbI	TGCGCA	1 cut(s)	999
NsiI	ATGCAT	3 cut(s)	407, 760, 970
NspI	RCATGY	1 cut(s)	1060
OliI	CACNNNNGTG	2 cut(s)	1154, 1578
PceI	AGGCCT	1 cut(s)	443
PciSI	GCTCTTC	2 cut(s)	1311, 1328
PctI	GAATGC	2 cut(s)	405, 1569
PdiI	GCCGGC	1 cut(s)	1187
PdmI	GAANNNNTTC	2 cut(s)	692, 1569
PfeI	GAWTC	3 cut(s)	254, 371, 1130
PkrI	GCNGC	6 cut(s)	120, 807, 895, 918, 1006, 1249
PshBI	ATTAAT	1 cut(s)	543
PsiI	TTATAA	2 cut(s)	437, 1605
Psp124BI	GAGCTC	2 cut(s)	296, 1323
PspN4I	GGNNCC	4 cut(s)	50, 70, 1593, 1804
PspPI	GGNCC	3 cut(s)	682, 1238, 1717
PstI	CTGCAG	1 cut(s)	898
PstNI	CAGNNNCTG	1 cut(s)	124
PsuI	RGATCY	4 cut(s)	68, 585, 1591, 1802
PvuII	CAGCTG	1 cut(s)	1250
RsaI	GTAC	3 cut(s)	135, 1261, 1482
RsaNI	GTAC	3 cut(s)	134, 1260, 1481
RseI	CAYNNNNRTG	4 cut(s)	993, 1154, 1578, 1631
SacI	GAGCTC	2 cut(s)	296, 1323
SapI	GCTCTTC	2 cut(s)	1311, 1328
SaqAI	TTAA	8 cut(s)	156, 216, 543, 644, 911, 1161, 1278, 1349
SatI	GCNGC	6 cut(s)	119, 806, 894, 917, 1005, 1248
Sau3AI	GATC	6 cut(s)	68, 585, 1507, 1591, 1635, 1802
Sau96I	GGNCC	3 cut(s)	682, 1238, 1717
ScaI	AGTACT	1 cut(s)	1482
ScrFI	CCNGG	2 cut(s)	73, 1135
SduI	GDGCHC	3 cut(s)	107, 296, 1323
SfaNI	GCATC	9 cut(s)	325, 392, 523, 953, 955, 985, 1113, 1406, 1435
SfcI	CTRYAG	1 cut(s)	894
SinI	GGWCC	2 cut(s)	1238, 1717
SmiMI	CAYNNNNRTG	4 cut(s)	993, 1154, 1578, 1631
SmlI	CTYRAG	1 cut(s)	1498
SmoI	CTYRAG	1 cut(s)	1498
SpeI	ACTAGT	1 cut(s)	1577
SseBI	AGGCCT	1 cut(s)	443
SsiI	CCGC	5 cut(s)	14, 496, 697, 1448, 1467
SspI	AATATT	1 cut(s)	798
SstI	GAGCTC	2 cut(s)	296, 1323
StuI	AGGCCT	1 cut(s)	443
StyD4I	CCNGG	2 cut(s)	71, 1133
StyI	CCWWGG	1 cut(s)	1743
TaaI	ACNGT	6 cut(s)	20, 693, 869, 1166, 1242, 1813
TaiI	ACGT	1 cut(s)	1792
TaqI	TCGA	5 cut(s)	400, 531, 871, 1305, 1794
TaqII	GACCGA	1 cut(s)	1705
TatI	WGTACW	2 cut(s)	1259, 1480
TfiI	GAWTC	3 cut(s)	254, 371, 1130
Tru1I	TTAA	8 cut(s)	156, 216, 543, 644, 911, 1161, 1278, 1349
Tru9I	TTAA	8 cut(s)	156, 216, 543, 644, 911, 1161, 1278, 1349
TscAI	CASTG	7 cut(s)	102, 115, 360, 1233, 1633, 1816, 1857
TseFI	GTSAC	1 cut(s)	1363
TseI	GCWGC	6 cut(s)	118, 805, 893, 916, 1004, 1247
Tsp45I	GTSAC	1 cut(s)	1363
TspDTI	ATGAA	4 cut(s)	614, 1005, 1034, 1713
TspRI	CASTG	7 cut(s)	102, 115, 360, 1233, 1633, 1816, 1857
VpaK11BI	GGWCC	2 cut(s)	1238, 1717
VspI	ATTAAT	1 cut(s)	543
XapI	RAATTY	6 cut(s)	87, 159, 900, 1597, 1619, 1757
XceI	RCATGY	1 cut(s)	1060
XmiI	GTMKAC	1 cut(s)	839
XmnI	GAANNNNTTC	2 cut(s)	692, 1569
ZraI	GACGTC	1 cut(s)	1790
ZrmI	AGTACT	1 cut(s)	1482
Zsp2I	ATGCAT	3 cut(s)	407, 760, 970

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.