Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0006156

Physical Location & Seq

Genomic Coordinates Reverse (-)

11943552 .. 11945886

Gene Structure 2335 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g18650.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGACAAAGCAGGTCTCTCTTGACCGTCGCCTCCAATCGCTCTTCCCTGTTGCATTGGCTTCTCTGCTTGTCCTCGGGACTACCCGACTATTCCTGGACACGTTGAAGAGCAACCATAGCTTTGTGTTTCAGCTATATGGCAGGCAAAGAGGTAAAGCAGAGAGACCAGCAGTGATTGTTTCCCCTGAGGATCGCATTGATGAAAGTTGCAATGTTTTTGAAGGAAATTGGGTATGGAATAACGAGTCAGGTCCTCCTCACTATACAGAAGAGAGCTGCCCCTACTTGGTGAAACAGGTTACATGCCAAAAGAATGGTAGGCCTGATTCTTATTACAAGAATTGGAGATGGCAACCCAATGACTGCAACCTCCCAAGGTTTGATGCATTGAAGCTACTGCAAATTCTGAGGGGCAAAAGACTGATGTTTGTTGGAGACTCGGTACAAAGAGGCCAGTTTGAATCCATGGTCTGCATGGTACAATCCATACTTCGTCAAGGAAAGAAATCCCTCCAAAGGGTTCCACCTAGGAAGATATTCAAAATCAAGGAGTTTGATGCCTCCATTGAGTACTACTGGGCTCCATTCATTGTGGAGTCAATTTCGGATCATGCAACGAAGCATACTGTACTAAAAAGGCTGGTGAAGCTTGACTCTATAGCCAAGCATGGCAAGCACTGGGAAGGAGTAGATATTTTGGTGTTTGAGAGCTATGTATGGTGGATGCACAAACCTACGATCAATGCTACATATGGAGCTCCAAATGATGTCCAAGAATATAATGTAACTACTGCTTATAAACTGGCATTACAAACTTGGGCAGAATGGTTAGAATCAAGCATAAACCCAGAAAGGCAAAAGGTGTTCTTCATGAGTATGTCTCCAACACATCTATGGAGCTGGGAATGGAGGCCTGGCAGTGATGAAAACTGCTATAATGAGTCCTATCCAATTCAAGGTTCATATTGGGGTACTGGTTCGAACACCAAAATAATGGATATTATAGACGACACTTTACAGGATTTGAAAGTTAAGGTCACATTTCTGAATATCACACAATTGTCTGAGTACAGAAAAGATGCACACACATCAGTTTATGGGGAACGCAAGGGCAAGCTCCTGACGAAGGAACAAAGATCTGATCCGAAAAACTTTGCCGATTGCATTCACTGGTGCTTACCTGGAGTTCCAGATACATGGAATGAAATTTTGTACGCACATTTGTTAAAGGGCCATCAAAATTTTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009987 GO:0010383 GO:0010410 GO:0012505 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0070589 GO:0070592 GO:0071554 GO:0071704 GO:0071840 GO:1901576 GO:1990538

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

417

Amino Acids

48.5

Weight (kDa)

8.71

Isoelectric Point (pI)

42.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	68 - 123	8.6e-21	PMR5 N terminal Domain
PC-Esterase	PF13839	124 - 298	2.4e-50	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006156)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73140
fragaria_vesca	FvH4_3g18650 FvH4_3g18650
malus_domestica	MD03G1240300.v1.1
prunus_persica	Prupe.4G170300_v2.0.a1 Prupe.4G170300_v2.0.a1
pyrus_communis	pycom03g18550
rosa_chinensis	RchiOBHm_Chr5g0031471 RchiOBHm_Chr7g0217351
rosa_laevigata	RLG00000033269 RLG00000033270 RLG00000033310
rosa_multiflora	Rmu_sc0000404.1_g000003 Rmu_sc0011824.1_g000011 Rmu_sc0038917.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048860 Rroxscaffold_3G00242310
rosa_rugosa	Rorug05G0126000 Rorug05G0126100 Rorug07G0168300
rosa_samantha	Rh5AG218900 Rh5BG218200 Rh5CG241900 Rh5DG223500 Rh7BG298600
rosa_wichuraiana	Rw5G019970 Rw7G026110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	798
AclWI	GGATC	3 cut(s)	198, 615, 1136
AcsI	RAATTY	3 cut(s)	402, 1206, 1240
AfaI	GTAC	7 cut(s)	444, 480, 572, 630, 973, 1070, 1214
AfiI	CCNNNNNNNGG	5 cut(s)	286, 516, 517, 956, 1126
AflIII	ACRYGT	1 cut(s)	99
AgsI	TTSAA	7 cut(s)	106, 221, 391, 461, 541, 956, 1027
AjnI	CCWGG	3 cut(s)	93, 913, 1180
AjuI	GAANNNNNNNTTGG	2 cut(s)	943, 975
AluBI	AGCT	9 cut(s)	120, 133, 276, 394, 649, 711, 758, 900, 1117
AluI	AGCT	9 cut(s)	120, 133, 276, 394, 649, 711, 758, 900, 1117
Alw21I	GWGCWC	1 cut(s)	760
Alw26I	GTCTC	4 cut(s)	19, 157, 429, 885
AlwI	GGATC	3 cut(s)	198, 615, 1136
Ama87I	CYCGRG	1 cut(s)	74
AoxI	GGCC	4 cut(s)	320, 451, 911, 1231
ApeKI	GCWGC	1 cut(s)	276
ApoI	RAATTY	3 cut(s)	402, 1206, 1240
Asp700I	GAANNNNTTC	1 cut(s)	536
AspA2I	CCTAGG	1 cut(s)	527
AspS9I	GGNCC	2 cut(s)	251, 1231
AsuHPI	GGTGA	2 cut(s)	301, 655
AsuII	TTCGAA	1 cut(s)	980
AvaI	CYCGRG	1 cut(s)	74
AvaII	GGWCC	1 cut(s)	251
AvrII	CCTAGG	1 cut(s)	527
AxyI	CCTNAGG	1 cut(s)	186
BanII	GRGCYC	2 cut(s)	583, 760
Bbv12I	GWGCWC	1 cut(s)	760
BbvI	GCAGC	1 cut(s)	263
BccI	CCATC	2 cut(s)	342, 1242
BciT130I	CCWGG	3 cut(s)	95, 915, 1182
BcoDI	GTCTC	4 cut(s)	19, 157, 429, 885
BfaI	CTAG	1 cut(s)	528
BfmI	CTRYAG	1 cut(s)	657
BglII	AGATCT	1 cut(s)	1136
BisI	GCNGC	1 cut(s)	277
BlnI	CCTAGG	1 cut(s)	527
BlsI	GCNGC	1 cut(s)	278
BmcAI	AGTACT	1 cut(s)	572
Bme1390I	CCNGG	3 cut(s)	95, 915, 1182
Bme18I	GGWCC	1 cut(s)	251
BmeT110I	CYCGRG	1 cut(s)	74
BmgT120I	GGNCC	2 cut(s)	251, 1231
BmiI	GGNNCC	2 cut(s)	522, 582
BmrFI	CCNGG	3 cut(s)	95, 915, 1182
BmrI	ACTGGG	2 cut(s)	586, 688
BmsI	GCATC	4 cut(s)	373, 547, 714, 1069
BmuI	ACTGGG	2 cut(s)	586, 688
BplI	GAGNNNNNCTC	2 cut(s)	865, 897
BpmI	CTGGAG	1 cut(s)	1203
Bpu14I	TTCGAA	1 cut(s)	980
BsaI	GGTCTC	2 cut(s)	19, 157
BsaJI	CCNNGG	4 cut(s)	73, 374, 465, 527
BsaXI	ACNNNNNCTCC	2 cut(s)	426, 456
Bsc4I	CCNNNNNNNGG	5 cut(s)	286, 516, 517, 956, 1126
Bse1I	ACTGG	6 cut(s)	454, 581, 683, 807, 979, 1175
Bse21I	CCTNAGG	1 cut(s)	186
Bse3DI	GCAATG	1 cut(s)	217
BseBI	CCWGG	3 cut(s)	95, 915, 1182
BseDI	CCNNGG	4 cut(s)	73, 374, 465, 527
BseGI	GGATG	1 cut(s)	729
BseLI	CCNNNNNNNGG	5 cut(s)	286, 516, 517, 956, 1126
BseMI	GCAATG	1 cut(s)	217
BseMII	CTCAG	3 cut(s)	177, 398, 1056
BseNI	ACTGG	6 cut(s)	454, 581, 683, 807, 979, 1175
BseRI	GAGGAG	1 cut(s)	246
BseXI	GCAGC	1 cut(s)	263
BseYI	CCCAGC	1 cut(s)	900
BshFI	GGCC	4 cut(s)	322, 453, 913, 1233
BsiHKAI	GWGCWC	1 cut(s)	760
BsiHKCI	CYCGRG	1 cut(s)	74
BslFI	GGGAC	1 cut(s)	91
BslI	CCNNNNNNNGG	5 cut(s)	286, 516, 517, 956, 1126
BsmAI	GTCTC	4 cut(s)	19, 157, 429, 885
BsmFI	GGGAC	1 cut(s)	91
BsmI	GAATGC	1 cut(s)	1164
BsnI	GGCC	4 cut(s)	322, 453, 913, 1233
Bso31I	GGTCTC	2 cut(s)	19, 157
BsoBI	CYCGRG	1 cut(s)	74
Bsp119I	TTCGAA	1 cut(s)	980
Bsp1286I	GDGCHC	2 cut(s)	583, 760
Bsp143I	GATC	5 cut(s)	190, 607, 738, 1136, 1141
Bsp19I	CCATGG	1 cut(s)	465
BspANI	GGCC	4 cut(s)	322, 453, 913, 1233
BspCNI	CTCAG	3 cut(s)	178, 399, 1057
BspHI	TCATGA	1 cut(s)	870
BspLI	GGNNCC	2 cut(s)	522, 582
BspPI	GGATC	3 cut(s)	198, 615, 1136
BspQI	GCTCTTC	2 cut(s)	47, 101
BspT104I	TTCGAA	1 cut(s)	980
BspTNI	GGTCTC	2 cut(s)	19, 157
BsrDI	GCAATG	1 cut(s)	217
BsrI	ACTGG	6 cut(s)	454, 581, 683, 807, 979, 1175
BssECI	CCNNGG	4 cut(s)	73, 374, 465, 527
BssMI	GATC	5 cut(s)	190, 607, 738, 1136, 1141
BssT1I	CCWWGG	3 cut(s)	374, 465, 527
Bst2UI	CCWGG	3 cut(s)	95, 915, 1182
Bst4CI	ACNGT	2 cut(s)	26, 628
Bst6I	CTCTTC	3 cut(s)	47, 101, 264
BstBI	TTCGAA	1 cut(s)	980
BstC8I	GCNNGC	3 cut(s)	143, 674, 1115
BstDEI	CTNAG	3 cut(s)	186, 407, 1065
BstDSI	CCRYGG	1 cut(s)	465
BstENI	CCTNNNNNAGG	1 cut(s)	1124
BstF5I	GGATG	1 cut(s)	729
BstKTI	GATC	5 cut(s)	193, 610, 741, 1139, 1144
BstMAI	GTCTC	4 cut(s)	19, 157, 429, 885
BstMBI	GATC	5 cut(s)	190, 607, 738, 1136, 1141
BstMWI	GCNNNNNNNGC	3 cut(s)	117, 646, 673
BstNI	CCWGG	3 cut(s)	95, 915, 1182
BstNSI	RCATGY	1 cut(s)	306
BstSCI	CCNGG	3 cut(s)	93, 913, 1180
BstSFI	CTRYAG	1 cut(s)	657
BstV1I	GCAGC	1 cut(s)	263
BstX2I	RGATCY	1 cut(s)	1136
BstXI	CCANNNNNNTGG	3 cut(s)	314, 994, 1197
BstYI	RGATCY	1 cut(s)	1136
Bsu36I	CCTNAGG	1 cut(s)	186
BsuRI	GGCC	4 cut(s)	322, 453, 913, 1233
BtgI	CCRYGG	1 cut(s)	465
BtsCI	GGATG	1 cut(s)	729
BtsI	GCAGTG	2 cut(s)	177, 925
BtsIMutI	CAGTG	4 cut(s)	177, 676, 925, 1168
Cac8I	GCNNGC	3 cut(s)	143, 674, 1115
CciI	TCATGA	1 cut(s)	870
Cfr13I	GGNCC	2 cut(s)	251, 1231
Csp6I	GTAC	7 cut(s)	443, 479, 571, 629, 972, 1069, 1213
CviAII	CATG	7 cut(s)	303, 466, 475, 611, 668, 871, 1197
CviQI	GTAC	7 cut(s)	443, 479, 571, 629, 972, 1069, 1213
DdeI	CTNAG	3 cut(s)	186, 407, 1065
DpnI	GATC	5 cut(s)	192, 609, 740, 1138, 1143
DpnII	GATC	5 cut(s)	190, 607, 738, 1136, 1141
Eam1104I	CTCTTC	3 cut(s)	47, 101, 264
EarI	CTCTTC	3 cut(s)	47, 101, 264
Ecl136II	GAGCTC	1 cut(s)	758
Eco130I	CCWWGG	3 cut(s)	374, 465, 527
Eco147I	AGGCCT	2 cut(s)	322, 913
Eco24I	GRGCYC	2 cut(s)	583, 760
Eco31I	GGTCTC	2 cut(s)	19, 157
Eco47I	GGWCC	1 cut(s)	251
Eco53kI	GAGCTC	1 cut(s)	758
Eco81I	CCTNAGG	1 cut(s)	186
Eco88I	CYCGRG	1 cut(s)	74
EcoICRI	GAGCTC	1 cut(s)	758
EcoNI	CCTNNNNNAGG	1 cut(s)	1124
EcoO109I	RGGNCCY	1 cut(s)	251
EcoRII	CCWGG	3 cut(s)	93, 913, 1180
EcoT14I	CCWWGG	3 cut(s)	374, 465, 527
EcoT22I	ATGCAT	1 cut(s)	388
EcoT38I	GRGCYC	2 cut(s)	583, 760
ErhI	CCWWGG	3 cut(s)	374, 465, 527
FaeI	CATG	7 cut(s)	306, 469, 478, 614, 671, 874, 1200
FalI	AAGNNNNNCTT	2 cut(s)	851, 883
FaqI	GGGAC	1 cut(s)	91
FatI	CATG	7 cut(s)	302, 465, 474, 610, 667, 870, 1196
FauNDI	CATATG	1 cut(s)	751
Fnu4HI	GCNGC	1 cut(s)	277
FokI	GGATG	1 cut(s)	736
FriOI	GRGCYC	2 cut(s)	583, 760
Fsp4HI	GCNGC	1 cut(s)	277
FspBI	CTAG	1 cut(s)	528
GluI	GCNGC	1 cut(s)	277
GsaI	CCCAGC	1 cut(s)	904
GsuI	CTGGAG	1 cut(s)	1203
HaeIII	GGCC	4 cut(s)	322, 453, 913, 1233
Hin1II	CATG	7 cut(s)	306, 469, 478, 614, 671, 874, 1200
HindIII	AAGCTT	1 cut(s)	647
HinfI	GANTC	8 cut(s)	245, 326, 437, 461, 596, 653, 833, 941
HphI	GGTGA	2 cut(s)	301, 655
Hpy188I	TCNGA	6 cut(s)	408, 607, 1047, 1066, 1141, 1146
Hpy188III	TCNNGA	5 cut(s)	20, 76, 871, 1120, 1190
Hpy99I	CGWCG	1 cut(s)	30
HpyAV	CCTTC	3 cut(s)	215, 677, 1120
HpyCH4III	ACNGT	2 cut(s)	26, 628
HpyCH4IV	ACGT	1 cut(s)	101
HpyF10VI	GCNNNNNNNGC	3 cut(s)	117, 646, 673
HpyF3I	CTNAG	3 cut(s)	186, 407, 1065
HpySE526I	ACGT	1 cut(s)	101
Hsp92II	CATG	7 cut(s)	306, 469, 478, 614, 671, 874, 1200
Kzo9I	GATC	5 cut(s)	190, 607, 738, 1136, 1141
LguI	GCTCTTC	2 cut(s)	47, 101
LmnI	GCTCC	5 cut(s)	586, 755, 763, 897, 1122
Lsp1109I	GCAGC	1 cut(s)	263
LweI	GCATC	4 cut(s)	373, 547, 714, 1069
MaeI	CTAG	1 cut(s)	528
MaeII	ACGT	1 cut(s)	101
MaeIII	GTNAC	3 cut(s)	298, 784, 1036
MalI	GATC	5 cut(s)	192, 609, 740, 1138, 1143
MboI	GATC	5 cut(s)	190, 607, 738, 1136, 1141
MboII	GAAGA	5 cut(s)	34, 118, 281, 544, 859
MfeI	CAATTG	1 cut(s)	1058
MflI	RGATCY	1 cut(s)	1136
MhlI	GDGCHC	2 cut(s)	583, 760
MluCI	AATT	8 cut(s)	226, 340, 402, 600, 951, 1058, 1206, 1240
MlyI	GAGTC	5 cut(s)	254, 431, 605, 647, 950
MmeI	TCCRAC	2 cut(s)	412, 908
Mph1103I	ATGCAT	1 cut(s)	388
MroXI	GAANNNNTTC	1 cut(s)	536
MseI	TTAA	2 cut(s)	1032, 1226
MslI	CAYNNNNRTG	2 cut(s)	875, 892
MspR9I	CCNGG	3 cut(s)	95, 915, 1182
MunI	CAATTG	1 cut(s)	1058
Mva1269I	GAATGC	1 cut(s)	1164
MvaI	CCWGG	3 cut(s)	95, 915, 1182
MwoI	GCNNNNNNNGC	3 cut(s)	117, 646, 673
NcoI	CCATGG	1 cut(s)	465
NdeI	CATATG	1 cut(s)	751
NdeII	GATC	5 cut(s)	190, 607, 738, 1136, 1141
NlaIII	CATG	7 cut(s)	306, 469, 478, 614, 671, 874, 1200
NlaIV	GGNNCC	2 cut(s)	522, 582
NmuCI	GTSAC	1 cut(s)	1036
NsiI	ATGCAT	1 cut(s)	388
NspI	RCATGY	1 cut(s)	306
NspV	TTCGAA	1 cut(s)	980
PagI	TCATGA	1 cut(s)	870
PceI	AGGCCT	2 cut(s)	322, 913
PciSI	GCTCTTC	2 cut(s)	47, 101
PctI	GAATGC	1 cut(s)	1164
PdmI	GAANNNNTTC	1 cut(s)	536
PfeI	GAWTC	3 cut(s)	326, 461, 833
PfoI	TCCNGGA	1 cut(s)	93
PkrI	GCNGC	1 cut(s)	278
PleI	GAGTC	5 cut(s)	253, 431, 604, 647, 949
PpsI	GAGTC	5 cut(s)	253, 431, 604, 647, 949
PpuMI	RGGWCCY	1 cut(s)	251
PsiI	TTATAA	1 cut(s)	798
Psp124BI	GAGCTC	1 cut(s)	760
Psp5II	RGGWCCY	1 cut(s)	251
Psp6I	CCWGG	3 cut(s)	93, 913, 1180
PspFI	CCCAGC	1 cut(s)	900
PspGI	CCWGG	3 cut(s)	93, 913, 1180
PspN4I	GGNNCC	2 cut(s)	522, 582
PspPI	GGNCC	2 cut(s)	251, 1231
PspPPI	RGGWCCY	1 cut(s)	251
PsuI	RGATCY	1 cut(s)	1136
RsaI	GTAC	7 cut(s)	444, 480, 572, 630, 973, 1070, 1214
RsaNI	GTAC	7 cut(s)	443, 479, 571, 629, 972, 1069, 1213
RseI	CAYNNNNRTG	2 cut(s)	875, 892
SacI	GAGCTC	1 cut(s)	760
SapI	GCTCTTC	2 cut(s)	47, 101
SaqAI	TTAA	2 cut(s)	1032, 1226
SatI	GCNGC	1 cut(s)	277
Sau3AI	GATC	5 cut(s)	190, 607, 738, 1136, 1141
Sau96I	GGNCC	2 cut(s)	251, 1231
ScaI	AGTACT	1 cut(s)	572
SchI	GAGTC	5 cut(s)	254, 431, 605, 647, 950
ScrFI	CCNGG	3 cut(s)	95, 915, 1182
SduI	GDGCHC	2 cut(s)	583, 760
SfaNI	GCATC	4 cut(s)	373, 547, 714, 1069
SfcI	CTRYAG	1 cut(s)	657
SfuI	TTCGAA	1 cut(s)	980
SinI	GGWCC	1 cut(s)	251
SmiMI	CAYNNNNRTG	2 cut(s)	875, 892
Sse9I	AATT	8 cut(s)	226, 340, 402, 600, 951, 1058, 1206, 1240
SseBI	AGGCCT	2 cut(s)	322, 913
SspMI	CTAG	1 cut(s)	528
SstI	GAGCTC	1 cut(s)	760
StuI	AGGCCT	2 cut(s)	322, 913
StyD4I	CCNGG	3 cut(s)	93, 913, 1180
StyI	CCWWGG	3 cut(s)	374, 465, 527
TaaI	ACNGT	2 cut(s)	26, 628
TaiI	ACGT	1 cut(s)	104
TaqI	TCGA	1 cut(s)	980
TasI	AATT	8 cut(s)	226, 340, 402, 600, 951, 1058, 1206, 1240
TatI	WGTACW	3 cut(s)	570, 628, 1068
TfiI	GAWTC	3 cut(s)	326, 461, 833
Tru1I	TTAA	2 cut(s)	1032, 1226
Tru9I	TTAA	2 cut(s)	1032, 1226
TscAI	CASTG	4 cut(s)	177, 683, 925, 1175
TseFI	GTSAC	1 cut(s)	1036
TseI	GCWGC	1 cut(s)	276
Tsp45I	GTSAC	1 cut(s)	1036
TspDTI	ATGAA	6 cut(s)	216, 577, 859, 939, 951, 1218
TspRI	CASTG	4 cut(s)	177, 683, 925, 1175
VpaK11BI	GGWCC	1 cut(s)	251
XagI	CCTNNNNNAGG	1 cut(s)	1124
XapI	RAATTY	3 cut(s)	402, 1206, 1240
XceI	RCATGY	1 cut(s)	306
XcmI	CCANNNNNNNNNTGG	2 cut(s)	472, 891
XmaJI	CCTAGG	1 cut(s)	527
XmnI	GAANNNNTTC	1 cut(s)	536
XspI	CTAG	1 cut(s)	528
ZrmI	AGTACT	1 cut(s)	572
Zsp2I	ATGCAT	1 cut(s)	388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.