Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006156

Physical Location & Seq

Genomic Coordinates Forward (+)

32561041 .. 32563095

Gene Structure 2055 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1240300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1245 bp

ATGACAAAGCAGCAGCCCTCCCTCGACCGGCGAATCCAGTCCCTCTTCCCGTTTGCACTGGCCTCGCTGCTTGTCCTAGGGACCGCACGCCTAGTCCTCGACGCCTTGAAGAGCAATGACAGCTTTGTCTTTCAGATGTATGGCTGGCCAAGAGGCCAGAAACCGAGACCGGCAGTTGTTATTTCACCGGAGGACCGGATTGACGAGAGCTGCAATGTGTTTGAAGGGAAGTGGGTGTGGGATAATTTGTCACATCCTCACTATACAGAAGAGAGCTGCCCCTACTTGGTAAAGCAAGTTACTTGCCAGAGGAATGGGAGGCCTGATTCTTACTACAAGAATTGGAGGTGGCAACCAAATGACTGCAACCTCCCAAGGTTTGATGCTTTGAAGCTAATGCAGATTTTGAGGGGCAAAAGGCTGATGTTTGTTGGAGACTCAGTACAAAGAGGCCAGTTTGAATCCCTGGTCTGCATGGTTCAATCTATAACACAAGGAAAAAGGTCCCTCCAAAGAGTCCCTCCTCGAAAGATCTTCAGAGTAGAGGAGTTTGATGCATCGATTGAGTACTACTGGGCTCCGTTTCTCGTGGAGTCTATTTCGGACCACGCGACGAAACATACTGTGCTGAAACGGCTGGTGAACCTTGACTCCATAACCAACCACGGCAAGCACTGGGAAGGAGTAGATATCTTGGTGTTTGAGAGCTATGTGTGGTGGATGCACAGCCCTACAGTCAATGCTACATATGGATCTCCAGATGTACAAGAATATAAAGTCACTACAGCATACAAACTAGCCTTACAAACTTGGGCAGACTGGTTAGAATCCAGCATCGACCCTGAAAGGCAAAAGGTCTTCTTCATGAGCATGTCTCCAACACATTTATGGAGTTGGGAATGGAAGCCGGGAAGTGACGAAAACTGCTTCAATGAGTCATACCCGATTGAGGGTTCATACTGGGGAACTGGTTCAAACATGGAAATCATGGATATTATACACGACCAAATGCAGGATCTGAAAGCGAAGGTAACATTTTTGAATATCACCCAATTGTCAGAGTACCGGAAAGATGCACACACATCGGTTTATGGAGAACGTCGGGGAAAGCTCTTGACGAAGAAACAAAGATCGGATCCTAAAAAATTCGCCGACTGCATTCACTGGTGCCTACCTGGGGTTCCTGATACATGGAATGAAATTTTGTATGCACATTTGATGAAGAGTCACCAAAATATTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009987 GO:0010383 GO:0010410 GO:0012505 GO:0016051 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044038 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045492 GO:0070589 GO:0070592 GO:0071554 GO:0071704 GO:0071840 GO:1901576 GO:1990538

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

415

Amino Acids

48.4

Weight (kDa)

7.63

Isoelectric Point (pI)

50.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	69 - 123	9.5e-22	PMR5 N terminal Domain
PC-Esterase	PF13839	124 - 404	4.2e-87	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006156)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73140
fragaria_vesca	FvH4_3g18650 FvH4_3g18650
malus_domestica	MD03G1240300.v1.1
prunus_persica	Prupe.4G170300_v2.0.a1 Prupe.4G170300_v2.0.a1
pyrus_communis	pycom03g18550
rosa_chinensis	RchiOBHm_Chr5g0031471 RchiOBHm_Chr7g0217351
rosa_laevigata	RLG00000033269 RLG00000033270 RLG00000033310
rosa_multiflora	Rmu_sc0000404.1_g000003 Rmu_sc0011824.1_g000011 Rmu_sc0038917.1_g000001
rosa_roxburghii	Rroxscaffold_1G00048860 Rroxscaffold_3G00242310
rosa_rugosa	Rorug05G0126000 Rorug05G0126100 Rorug07G0168300
rosa_samantha	Rh5AG218900 Rh5BG218200 Rh5CG241900 Rh5DG223500 Rh7BG298600
rosa_wichuraiana	Rw5G019970 Rw7G026110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	125
AccB1I	GGYRCC	1 cut(s)	1167
AccII	CGCG	1 cut(s)	611
AciI	CCGC	1 cut(s)	84
AclWI	GGATC	4 cut(s)	760, 1023, 1130, 1143
AcoI	YGGCCR	1 cut(s)	146
AcsI	RAATTY	2 cut(s)	1145, 1200
AcuI	CTGAAG	1 cut(s)	520
AcyI	GRCGYC	1 cut(s)	102
AfaI	GTAC	4 cut(s)	444, 569, 765, 1064
AfiI	CCNNNNNNNGG	6 cut(s)	27, 286, 949, 950, 1012, 1177
AgsI	TTSAA	8 cut(s)	109, 224, 391, 461, 482, 931, 975, 1042
AjnI	CCWGG	2 cut(s)	465, 1174
AloI	GAACNNNNNNTCC	2 cut(s)	635, 667
AluBI	AGCT	6 cut(s)	123, 210, 276, 394, 708, 1111
AluI	AGCT	6 cut(s)	123, 210, 276, 394, 708, 1111
Alw26I	GTCTC	3 cut(s)	160, 429, 879
AlwI	GGATC	4 cut(s)	760, 1023, 1130, 1143
AlwNI	CAGNNNCTG	1 cut(s)	1018
AoxI	GGCC	5 cut(s)	60, 146, 154, 320, 451
ApeKI	GCWGC	5 cut(s)	10, 13, 67, 210, 276
ApoI	RAATTY	2 cut(s)	1145, 1200
Asp700I	GAANNNNTTC	1 cut(s)	970
AspA2I	CCTAGG	1 cut(s)	76
AspS9I	GGNCC	4 cut(s)	81, 193, 504, 604
AsuC2I	CCSGG	1 cut(s)	909
AsuHPI	GGTGA	4 cut(s)	177, 652, 1039, 1220
AvaII	GGWCC	4 cut(s)	81, 193, 504, 604
AvrII	CCTAGG	1 cut(s)	76
BalI	TGGCCA	1 cut(s)	148
BamHI	GGATCC	1 cut(s)	1135
BanI	GGYRCC	1 cut(s)	1167
BanII	GRGCYC	1 cut(s)	580
BauI	CACGAG	1 cut(s)	587
BbsI	GAAGAC	1 cut(s)	850
BbvI	GCAGC	5 cut(s)	22, 25, 54, 197, 263
BceAI	ACGGC	2 cut(s)	650, 682
BcgI	CGANNNNNNTGC	4 cut(s)	45, 79, 1065, 1099
BciT130I	CCWGG	2 cut(s)	467, 1176
BcnI	CCSGG	1 cut(s)	909
BcoDI	GTCTC	3 cut(s)	160, 429, 879
BfaI	CTAG	3 cut(s)	77, 92, 797
BfmI	CTRYAG	2 cut(s)	732, 783
BglII	AGATCT	1 cut(s)	531
BisI	GCNGC	5 cut(s)	11, 14, 68, 211, 277
BlnI	CCTAGG	1 cut(s)	76
BlsI	GCNGC	5 cut(s)	12, 15, 69, 212, 278
BmcAI	AGTACT	1 cut(s)	569
Bme1390I	CCNGG	3 cut(s)	467, 909, 1176
Bme18I	GGWCC	4 cut(s)	81, 193, 504, 604
BmgT120I	GGNCC	4 cut(s)	81, 193, 504, 604
BmiI	GGNNCC	6 cut(s)	82, 506, 579, 1137, 1169, 1182
BmrFI	CCNGG	3 cut(s)	467, 909, 1176
BmrI	ACTGGG	3 cut(s)	583, 685, 970
BmsI	GCATC	6 cut(s)	373, 544, 566, 711, 843, 1063
BmuI	ACTGGG	3 cut(s)	583, 685, 970
BpiI	GAAGAC	1 cut(s)	850
BplI	GAGNNNNNCTC	2 cut(s)	859, 891
BpmI	CTGGAG	1 cut(s)	741
BpuMI	CCSGG	1 cut(s)	909
Bsa29I	ATCGAT	1 cut(s)	560
BsaHI	GRCGYC	1 cut(s)	102
BsaI	GGTCTC	1 cut(s)	160
BsaJI	CCNNGG	5 cut(s)	76, 374, 465, 664, 1175
BsaWI	WCCGGW	3 cut(s)	187, 195, 1065
BsaXI	ACNNNNNCTCC	4 cut(s)	426, 456, 635, 665
Bsc4I	CCNNNNNNNGG	6 cut(s)	27, 286, 949, 950, 1012, 1177
Bse118I	RCCGGY	2 cut(s)	27, 169
Bse1I	ACTGG	9 cut(s)	37, 63, 454, 578, 680, 824, 965, 973, 1169
Bse3DI	GCAATG	2 cut(s)	121, 220
BseBI	CCWGG	2 cut(s)	467, 1176
BseCI	ATCGAT	1 cut(s)	560
BseDI	CCNNGG	5 cut(s)	76, 374, 465, 664, 1175
BseGI	GGATG	2 cut(s)	253, 726
BseLI	CCNNNNNNNGG	6 cut(s)	27, 286, 949, 950, 1012, 1177
BseMI	GCAATG	2 cut(s)	121, 220
BseMII	CTCAG	1 cut(s)	453
BseNI	ACTGG	9 cut(s)	37, 63, 454, 578, 680, 824, 965, 973, 1169
BseRI	GAGGAG	2 cut(s)	513, 560
BseXI	GCAGC	5 cut(s)	22, 25, 54, 197, 263
Bsh1236I	CGCG	1 cut(s)	611
Bsh1285I	CGRYCG	1 cut(s)	28
BshFI	GGCC	5 cut(s)	62, 148, 156, 322, 453
BshNI	GGYRCC	1 cut(s)	1167
BshVI	ATCGAT	1 cut(s)	560
BsiEI	CGRYCG	1 cut(s)	28
BsiSI	CCGG	6 cut(s)	28, 170, 188, 196, 908, 1066
BslFI	GGGAC	4 cut(s)	25, 94, 490, 503
BslI	CCNNNNNNNGG	6 cut(s)	27, 286, 949, 950, 1012, 1177
BsmAI	GTCTC	3 cut(s)	160, 429, 879
BsmFI	GGGAC	4 cut(s)	25, 94, 490, 503
BsmI	GAATGC	1 cut(s)	1158
BsnI	GGCC	5 cut(s)	62, 148, 156, 322, 453
Bso31I	GGTCTC	1 cut(s)	160
Bsp1286I	GDGCHC	1 cut(s)	580
Bsp1407I	TGTACA	1 cut(s)	763
Bsp143I	GATC	5 cut(s)	531, 752, 1015, 1130, 1135
BspACI	CCGC	1 cut(s)	84
BspANI	GGCC	5 cut(s)	62, 148, 156, 322, 453
BspCNI	CTCAG	1 cut(s)	452
BspDI	ATCGAT	1 cut(s)	560
BspFNI	CGCG	1 cut(s)	611
BspHI	TCATGA	1 cut(s)	864
BspLI	GGNNCC	6 cut(s)	82, 506, 579, 1137, 1169, 1182
BspPI	GGATC	4 cut(s)	760, 1023, 1130, 1143
BspQI	GCTCTTC	1 cut(s)	104
BspT107I	GGYRCC	1 cut(s)	1167
BspTNI	GGTCTC	1 cut(s)	160
BsrDI	GCAATG	2 cut(s)	121, 220
BsrFI	RCCGGY	2 cut(s)	27, 169
BsrGI	TGTACA	1 cut(s)	763
BsrI	ACTGG	9 cut(s)	37, 63, 454, 578, 680, 824, 965, 973, 1169
BssAI	RCCGGY	2 cut(s)	27, 169
BssECI	CCNNGG	5 cut(s)	76, 374, 465, 664, 1175
BssMI	GATC	5 cut(s)	531, 752, 1015, 1130, 1135
BssNI	GRCGYC	1 cut(s)	102
BssSI	CACGAG	1 cut(s)	587
BssT1I	CCWWGG	2 cut(s)	76, 374
Bst2BI	CACGAG	1 cut(s)	587
Bst2UI	CCWGG	2 cut(s)	467, 1176
Bst4CI	ACNGT	2 cut(s)	625, 736
Bst6I	CTCTTC	4 cut(s)	50, 104, 264, 1217
BstACI	GRCGYC	1 cut(s)	102
BstAUI	TGTACA	1 cut(s)	763
BstC8I	GCNNGC	3 cut(s)	88, 146, 671
BstDEI	CTNAG	1 cut(s)	439
BstDSI	CCRYGG	1 cut(s)	664
BstF5I	GGATG	2 cut(s)	253, 726
BstFNI	CGCG	1 cut(s)	611
BstKTI	GATC	5 cut(s)	534, 755, 1018, 1133, 1138
BstMAI	GTCTC	3 cut(s)	160, 429, 879
BstMBI	GATC	5 cut(s)	531, 752, 1015, 1130, 1135
BstMCI	CGRYCG	1 cut(s)	28
BstMWI	GCNNNNNNNGC	2 cut(s)	120, 634
BstNI	CCWGG	2 cut(s)	467, 1176
BstNSI	RCATGY	1 cut(s)	874
BstSCI	CCNGG	3 cut(s)	465, 907, 1174
BstSFI	CTRYAG	2 cut(s)	732, 783
BstUI	CGCG	1 cut(s)	611
BstV1I	GCAGC	5 cut(s)	22, 25, 54, 197, 263
BstV2I	GAAGAC	1 cut(s)	850
BstX2I	RGATCY	4 cut(s)	531, 752, 1015, 1135
BstXI	CCANNNNNNTGG	1 cut(s)	314
BstYI	RGATCY	4 cut(s)	531, 752, 1015, 1135
Bsu15I	ATCGAT	1 cut(s)	560
BsuRI	GGCC	5 cut(s)	62, 148, 156, 322, 453
BsuTUI	ATCGAT	1 cut(s)	560
BtgI	CCRYGG	1 cut(s)	664
BtsCI	GGATG	2 cut(s)	253, 726
BtsIMutI	CAGTG	3 cut(s)	56, 673, 1162
Cac8I	GCNNGC	3 cut(s)	88, 146, 671
CaiI	CAGNNNCTG	1 cut(s)	1018
CciI	TCATGA	1 cut(s)	864
Cfr10I	RCCGGY	2 cut(s)	27, 169
Cfr13I	GGNCC	4 cut(s)	81, 193, 504, 604
ClaI	ATCGAT	1 cut(s)	560
CseI	GACGC	1 cut(s)	110
Csp6I	GTAC	4 cut(s)	443, 568, 764, 1063
CviAII	CATG	6 cut(s)	475, 865, 871, 979, 988, 1191
CviQI	GTAC	4 cut(s)	443, 568, 764, 1063
DdeI	CTNAG	1 cut(s)	439
DpnI	GATC	5 cut(s)	533, 754, 1017, 1132, 1137
DpnII	GATC	5 cut(s)	531, 752, 1015, 1130, 1135
DrdI	GACNNNNNNGTC	1 cut(s)	125
DseDI	GACNNNNNNGTC	1 cut(s)	125
EaeI	YGGCCR	1 cut(s)	146
Eam1104I	CTCTTC	4 cut(s)	50, 104, 264, 1217
EarI	CTCTTC	4 cut(s)	50, 104, 264, 1217
Eco130I	CCWWGG	2 cut(s)	76, 374
Eco147I	AGGCCT	1 cut(s)	322
Eco24I	GRGCYC	1 cut(s)	580
Eco31I	GGTCTC	1 cut(s)	160
Eco32I	GATATC	1 cut(s)	691
Eco47I	GGWCC	4 cut(s)	81, 193, 504, 604
Eco57I	CTGAAG	1 cut(s)	520
EcoO109I	RGGNCCY	1 cut(s)	504
EcoRII	CCWGG	2 cut(s)	465, 1174
EcoRV	GATATC	1 cut(s)	691
EcoT14I	CCWWGG	2 cut(s)	76, 374
EcoT22I	ATGCAT	1 cut(s)	559
EcoT38I	GRGCYC	1 cut(s)	580
ErhI	CCWWGG	2 cut(s)	76, 374
FaeI	CATG	6 cut(s)	478, 868, 874, 982, 991, 1194
FalI	AAGNNNNNCTT	2 cut(s)	845, 877
FaqI	GGGAC	4 cut(s)	25, 94, 490, 503
FatI	CATG	6 cut(s)	474, 864, 870, 978, 987, 1190
FauNDI	CATATG	1 cut(s)	748
Fnu4HI	GCNGC	5 cut(s)	11, 14, 68, 211, 277
FokI	GGATG	2 cut(s)	240, 733
FriOI	GRGCYC	1 cut(s)	580
Fsp4HI	GCNGC	5 cut(s)	11, 14, 68, 211, 277
FspBI	CTAG	3 cut(s)	77, 92, 797
GluI	GCNGC	5 cut(s)	11, 14, 68, 211, 277
GsuI	CTGGAG	1 cut(s)	741
HaeIII	GGCC	5 cut(s)	62, 148, 156, 322, 453
HapII	CCGG	6 cut(s)	28, 170, 188, 196, 908, 1066
HgaI	GACGC	1 cut(s)	110
Hin1I	GRCGYC	1 cut(s)	102
Hin1II	CATG	6 cut(s)	478, 868, 874, 982, 991, 1194
HpaII	CCGG	6 cut(s)	28, 170, 188, 196, 908, 1066
HphI	GGTGA	4 cut(s)	177, 652, 1039, 1220
Hpy166II	GTNNAC	1 cut(s)	643
Hpy188I	TCNGA	6 cut(s)	135, 539, 604, 1020, 1060, 1135
Hpy188III	TCNNGA	4 cut(s)	758, 865, 1114, 1184
Hpy8I	GTNNAC	1 cut(s)	643
Hpy99I	CGWCG	3 cut(s)	104, 616, 1104
HpyAV	CCTTC	3 cut(s)	218, 674, 1021
HpyCH4III	ACNGT	2 cut(s)	625, 736
HpyCH4IV	ACGT	1 cut(s)	1099
HpyF10VI	GCNNNNNNNGC	2 cut(s)	120, 634
HpyF3I	CTNAG	1 cut(s)	439
HpySE526I	ACGT	1 cut(s)	1099
Hsp92I	GRCGYC	1 cut(s)	102
Hsp92II	CATG	6 cut(s)	478, 868, 874, 982, 991, 1194
Kzo9I	GATC	5 cut(s)	531, 752, 1015, 1130, 1135
LguI	GCTCTTC	1 cut(s)	104
LmnI	GCTCC	1 cut(s)	583
Lsp1109I	GCAGC	5 cut(s)	22, 25, 54, 197, 263
LweI	GCATC	6 cut(s)	373, 544, 566, 711, 843, 1063
MaeI	CTAG	3 cut(s)	77, 92, 797
MaeII	ACGT	1 cut(s)	1099
MaeIII	GTNAC	6 cut(s)	249, 298, 778, 914, 1030, 1226
MalI	GATC	5 cut(s)	533, 754, 1017, 1132, 1137
MboI	GATC	5 cut(s)	531, 752, 1015, 1130, 1135
MboII	GAAGA	8 cut(s)	37, 121, 281, 526, 850, 853, 1132, 1234
MfeI	CAATTG	1 cut(s)	1052
MflI	RGATCY	4 cut(s)	531, 752, 1015, 1135
MhlI	GDGCHC	1 cut(s)	580
MlsI	TGGCCA	1 cut(s)	148
MluCI	AATT	5 cut(s)	244, 340, 1052, 1145, 1200
MluNI	TGGCCA	1 cut(s)	148
MlyI	GAGTC	6 cut(s)	431, 525, 602, 644, 944, 1234
MmeI	TCCRAC	2 cut(s)	412, 902
Mox20I	TGGCCA	1 cut(s)	148
Mph1103I	ATGCAT	1 cut(s)	559
MroXI	GAANNNNTTC	1 cut(s)	970
MscI	TGGCCA	1 cut(s)	148
MslI	CAYNNNNRTG	2 cut(s)	869, 886
Msp20I	TGGCCA	1 cut(s)	148
MspI	CCGG	6 cut(s)	28, 170, 188, 196, 908, 1066
MspR9I	CCNGG	3 cut(s)	467, 909, 1176
MunI	CAATTG	1 cut(s)	1052
Mva1269I	GAATGC	1 cut(s)	1158
MvaI	CCWGG	2 cut(s)	467, 1176
MvnI	CGCG	1 cut(s)	611
MwoI	GCNNNNNNNGC	2 cut(s)	120, 634
NciI	CCSGG	1 cut(s)	909
NdeI	CATATG	1 cut(s)	748
NdeII	GATC	5 cut(s)	531, 752, 1015, 1130, 1135
NlaIII	CATG	6 cut(s)	478, 868, 874, 982, 991, 1194
NlaIV	GGNNCC	6 cut(s)	82, 506, 579, 1137, 1169, 1182
NmuCI	GTSAC	4 cut(s)	249, 778, 914, 1226
NsiI	ATGCAT	1 cut(s)	559
NspI	RCATGY	1 cut(s)	874
PagI	TCATGA	1 cut(s)	864
PceI	AGGCCT	1 cut(s)	322
PciSI	GCTCTTC	1 cut(s)	104
PcsI	WCGNNNNNNNCGW	1 cut(s)	608
PctI	GAATGC	1 cut(s)	1158
PdmI	GAANNNNTTC	1 cut(s)	970
PfeI	GAWTC	4 cut(s)	33, 326, 461, 827
PkrI	GCNGC	5 cut(s)	12, 15, 69, 212, 278
PleI	GAGTC	6 cut(s)	431, 524, 601, 644, 943, 1233
PpsI	GAGTC	6 cut(s)	431, 524, 601, 644, 943, 1233
PpuMI	RGGWCCY	1 cut(s)	504
Psp5II	RGGWCCY	1 cut(s)	504
Psp6I	CCWGG	2 cut(s)	465, 1174
PspGI	CCWGG	2 cut(s)	465, 1174
PspN4I	GGNNCC	6 cut(s)	82, 506, 579, 1137, 1169, 1182
PspPI	GGNCC	4 cut(s)	81, 193, 504, 604
PspPPI	RGGWCCY	1 cut(s)	504
PstNI	CAGNNNCTG	1 cut(s)	1018
PsuI	RGATCY	4 cut(s)	531, 752, 1015, 1135
RsaI	GTAC	4 cut(s)	444, 569, 765, 1064
RsaNI	GTAC	4 cut(s)	443, 568, 764, 1063
RseI	CAYNNNNRTG	2 cut(s)	869, 886
SapI	GCTCTTC	1 cut(s)	104
SatI	GCNGC	5 cut(s)	11, 14, 68, 211, 277
Sau3AI	GATC	5 cut(s)	531, 752, 1015, 1130, 1135
Sau96I	GGNCC	4 cut(s)	81, 193, 504, 604
ScaI	AGTACT	1 cut(s)	569
SchI	GAGTC	6 cut(s)	431, 525, 602, 644, 944, 1234
ScrFI	CCNGG	3 cut(s)	467, 909, 1176
SduI	GDGCHC	1 cut(s)	580
SfaNI	GCATC	6 cut(s)	373, 544, 566, 711, 843, 1063
SfcI	CTRYAG	2 cut(s)	732, 783
SinI	GGWCC	4 cut(s)	81, 193, 504, 604
SmiMI	CAYNNNNRTG	2 cut(s)	869, 886
Sse9I	AATT	5 cut(s)	244, 340, 1052, 1145, 1200
SseBI	AGGCCT	1 cut(s)	322
SsiI	CCGC	1 cut(s)	84
SspI	AATATT	1 cut(s)	1237
SspMI	CTAG	3 cut(s)	77, 92, 797
StuI	AGGCCT	1 cut(s)	322
StyD4I	CCNGG	3 cut(s)	465, 907, 1174
StyI	CCWWGG	2 cut(s)	76, 374
TaaI	ACNGT	2 cut(s)	625, 736
TaiI	ACGT	1 cut(s)	1102
TaqI	TCGA	5 cut(s)	24, 99, 526, 560, 837
TasI	AATT	5 cut(s)	244, 340, 1052, 1145, 1200
TatI	WGTACW	3 cut(s)	442, 567, 763
TfiI	GAWTC	4 cut(s)	33, 326, 461, 827
TscAI	CASTG	3 cut(s)	63, 680, 1169
TseFI	GTSAC	4 cut(s)	249, 778, 914, 1226
TseI	GCWGC	5 cut(s)	10, 13, 67, 210, 276
Tsp45I	GTSAC	4 cut(s)	249, 778, 914, 1226
TspDTI	ATGAA	4 cut(s)	853, 945, 1212, 1235
TspGWI	ACGGA	1 cut(s)	570
TspRI	CASTG	3 cut(s)	63, 680, 1169
VpaK11BI	GGWCC	4 cut(s)	81, 193, 504, 604
XapI	RAATTY	2 cut(s)	1145, 1200
XceI	RCATGY	1 cut(s)	874
XcmI	CCANNNNNNNNNTGG	1 cut(s)	885
XmaJI	CCTAGG	1 cut(s)	76
XmnI	GAANNNNTTC	1 cut(s)	970
XspI	CTAG	3 cut(s)	77, 92, 797
ZrmI	AGTACT	1 cut(s)	569
Zsp2I	ATGCAT	1 cut(s)	559

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.