FvH4_3g19800

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010543
Physical Location & Seq
Reverse (-)
12956668 .. 12957453
786 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19800.t1

Sequence Viewer

Length: 786 bp
ATGGAGTACACATCAAGGCCTCAGGAACAAATCAGAGATCACCACCACCACCATCATCAGAAGCCGCCTCCTTCCTATTCTCCTCTAAACTCCCCCACCAGCAATGGCAGCAGCAATAACAATAACAACAACAACAACCACAATAATTCCGGAGTTCAAACTCCGACGACCCCCAAGCTCATGACCCCCAGATCTGACTCCAACCCTTACGCCACCACCTTCGTCCAGGCTGACTCCTCCAACTTCAAACACGTCGTCCAAATGCTCACCGGCTCATCCGAGACCACAACCAAGCAGCAAACCAACAAAGACCCCTCATCCTTATCCTCATCACCGCTCTCGTCGTCCAAGAACTTCACCATCCCGCCGGTGAAGACCGCCCCGAAGAAGCAGCAGGGCTTCAAGCTCTACGAGAGGAGGAGCCAGAACCTCAAGAACAGCCTCATGATCAATACCCTGATGCCCAATTTCGCCCACCACCGCACCTCCTCTGGGTTCTCGCCACGTCACCCGGCAGAGATCTTATCTCCGAGTCTGCTTGATTTTCCGTCGCTGACTCTCAGTCCGGTGACCCCATTGAACGACGAACATTTCGACAAGTCGTCGTCGTCGCCGATGGGGAACTCGTCGTCGGAGGAGGATCGGGCTATAGCCGAGAAGGGGTTTTACTTGCACCCTTCTCCGAGAACGAGCAGCAGCACTCCCAGAGAGCCCGAGCCCCGGCTTCTGCCATTGTTTCCGGTCACTTCGCCGAGAGTTTCGGGGTCGACGACGTCTACTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

262

Amino Acids

28.77

Weight (kDa)

9.58

Isoelectric Point (pI)

65.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 69 - 95 2.9e-09 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 776
AccBSI CCGCTC 1 cut(s) 337
AccI GTMKAC 2 cut(s) 767, 776
AccIII TCCGGA 1 cut(s) 149
AciI CCGC 5 cut(s) 65, 335, 365, 378, 481
AclWI GGATC 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 773
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 3 cut(s) 492, 660, 720
AflIII ACRYGT 1 cut(s) 250
AgsI TTSAA 4 cut(s) 158, 247, 403, 580
AhdI GACNNNNNGTC 2 cut(s) 561, 601
AjiI CACGTC 2 cut(s) 253, 506
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 2 cut(s) 178, 406
AluI AGCT 2 cut(s) 178, 406
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 1 cut(s) 648
Ama87I CYCGRG 1 cut(s) 713
Aor13HI TCCGGA 1 cut(s) 149
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 6 cut(s) 108, 111, 295, 391, 693, 696
ArsI GACNNNNNNTTYG 4 cut(s) 240, 272, 575, 607
AsuC2I CCSGG 2 cut(s) 512, 721
AsuHPI GGTGA 7 cut(s) 32, 259, 324, 349, 382, 500, 580
AvaI CYCGRG 1 cut(s) 713
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 2 cut(s) 714, 720
BarI GAAGNNNNNNTAC 2 cut(s) 650, 682
BbsI GAAGAC 1 cut(s) 380
BbvI GCAGC 6 cut(s) 120, 123, 307, 403, 705, 708
BccI CCATC 3 cut(s) 60, 368, 610
BciT130I CCWGG 1 cut(s) 227
BclI TGATCA 1 cut(s) 447
BcnI CCSGG 2 cut(s) 512, 721
BcoDI GTCTC 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 648
BglII AGATCT 2 cut(s) 191, 519
BisI GCNGC 7 cut(s) 65, 109, 112, 296, 392, 694, 697
BlsI GCNGC 7 cut(s) 66, 110, 113, 297, 393, 695, 698
Bme1390I CCNGG 3 cut(s) 227, 512, 721
BmeRI GACNNNNNGTC 2 cut(s) 561, 601
BmeT110I CYCGRG 1 cut(s) 713
BmgBI CACGTC 2 cut(s) 253, 506
BmiI GGNNCC 1 cut(s) 422
BmrFI CCNGG 3 cut(s) 227, 512, 721
BmsI GCATC 1 cut(s) 450
BpiI GAAGAC 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 2 cut(s) 512, 721
BsaHI GRCGYC 1 cut(s) 773
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 1 cut(s) 719
BsaWI WCCGGW 3 cut(s) 149, 565, 739
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 3 cut(s) 492, 660, 720
Bse118I RCCGGY 2 cut(s) 269, 367
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 109
BseAI TCCGGA 1 cut(s) 149
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 719
BseGI GGATG 3 cut(s) 275, 317, 360
BseLI CCNNNNNNNGG 3 cut(s) 492, 660, 720
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 35, 574
BseRI GAGGAG 6 cut(s) 72, 226, 430, 433, 478, 650
BseXI GCAGC 6 cut(s) 120, 123, 307, 403, 705, 708
BshFI GGCC 1 cut(s) 19
BsiHKCI CYCGRG 1 cut(s) 713
BsiSI CCGG 7 cut(s) 150, 270, 368, 512, 566, 721, 740
BslI CCNNNNNNNGG 3 cut(s) 492, 660, 720
BsmAI GTCTC 1 cut(s) 275
BsnI GGCC 1 cut(s) 19
Bso31I GGTCTC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 713
Bsp1286I GDGCHC 2 cut(s) 714, 720
Bsp13I TCCGGA 1 cut(s) 149
Bsp143I GATC 5 cut(s) 37, 191, 447, 519, 640
BspACI CCGC 5 cut(s) 65, 335, 365, 378, 481
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 2 cut(s) 34, 573
BspEI TCCGGA 1 cut(s) 149
BspHI TCATGA 2 cut(s) 180, 444
BspLI GGNNCC 1 cut(s) 422
BspPI GGATC 1 cut(s) 648
BspTNI GGTCTC 1 cut(s) 275
BsrBI CCGCTC 1 cut(s) 337
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 2 cut(s) 269, 367
BssAI RCCGGY 2 cut(s) 269, 367
BssECI CCNNGG 1 cut(s) 719
BssMI GATC 5 cut(s) 37, 191, 447, 519, 640
BssNI GRCGYC 1 cut(s) 773
Bst2UI CCWGG 1 cut(s) 227
BstACI GRCGYC 1 cut(s) 773
BstDEI CTNAG 2 cut(s) 21, 560
BstEII GGTNACC 1 cut(s) 568
BstF5I GGATG 3 cut(s) 275, 317, 360
BstKTI GATC 5 cut(s) 40, 194, 450, 522, 643
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 5 cut(s) 37, 191, 447, 519, 640
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNI CCWGG 1 cut(s) 227
BstPI GGTNACC 1 cut(s) 568
BstSCI CCNGG 3 cut(s) 225, 510, 719
BstSFI CTRYAG 1 cut(s) 648
BstV1I GCAGC 6 cut(s) 120, 123, 307, 403, 705, 708
BstV2I GAAGAC 1 cut(s) 380
BstX2I RGATCY 2 cut(s) 191, 519
BstYI RGATCY 2 cut(s) 191, 519
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 1 cut(s) 19
BtrI CACGTC 2 cut(s) 253, 506
BtsCI GGATG 3 cut(s) 275, 317, 360
CciI TCATGA 2 cut(s) 180, 444
Cfr10I RCCGGY 2 cut(s) 269, 367
Csp6I GTAC 1 cut(s) 7
CviAII CATG 2 cut(s) 181, 445
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 2 cut(s) 21, 560
DpnI GATC 5 cut(s) 39, 193, 449, 521, 642
DpnII GATC 5 cut(s) 37, 191, 447, 519, 640
DriI GACNNNNNGTC 2 cut(s) 561, 601
Eam1105I GACNNNNNGTC 2 cut(s) 561, 601
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 2 cut(s) 714, 720
Eco31I GGTCTC 1 cut(s) 275
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 713
Eco91I GGTNACC 1 cut(s) 568
EcoO65I GGTNACC 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 225
EcoT38I GRGCYC 2 cut(s) 714, 720
FaeI CATG 2 cut(s) 184, 448
FaiI YATR 3 cut(s) 182, 446, 650
FatI CATG 2 cut(s) 180, 444
FauI CCCGC 1 cut(s) 372
FbaI TGATCA 1 cut(s) 447
FblI GTMKAC 2 cut(s) 767, 776
Fnu4HI GCNGC 7 cut(s) 65, 109, 112, 296, 392, 694, 697
FokI GGATG 3 cut(s) 262, 304, 347
FriOI GRGCYC 2 cut(s) 714, 720
Fsp4HI GCNGC 7 cut(s) 65, 109, 112, 296, 392, 694, 697
GluI GCNGC 7 cut(s) 65, 109, 112, 296, 392, 694, 697
HaeIII GGCC 1 cut(s) 19
HapII CCGG 7 cut(s) 150, 270, 368, 512, 566, 721, 740
Hin1I GRCGYC 1 cut(s) 773
Hin1II CATG 2 cut(s) 184, 448
HincII GTYRAC 1 cut(s) 768
HindII GTYRAC 1 cut(s) 768
HinfI GANTC 4 cut(s) 197, 233, 532, 556
HpaII CCGG 7 cut(s) 150, 270, 368, 512, 566, 721, 740
HphI GGTGA 7 cut(s) 32, 259, 324, 349, 382, 500, 580
Hpy166II GTNNAC 3 cut(s) 9, 768, 777
Hpy188I TCNGA 8 cut(s) 35, 60, 165, 196, 280, 531, 634, 684
Hpy188III TCNNGA 6 cut(s) 23, 150, 181, 433, 445, 783
Hpy8I GTNNAC 3 cut(s) 9, 768, 777
HpyAV CCTTC 4 cut(s) 81, 229, 652, 687
HpyCH4IV ACGT 3 cut(s) 252, 505, 773
HpyCH4V TGCA 1 cut(s) 673
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 2 cut(s) 21, 560
HpySE526I ACGT 3 cut(s) 252, 505, 773
Hsp92I GRCGYC 1 cut(s) 773
Hsp92II CATG 2 cut(s) 184, 448
Kpn2I TCCGGA 1 cut(s) 149
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 5 cut(s) 37, 191, 447, 519, 640
LmnI GCTCC 1 cut(s) 420
Lsp1109I GCAGC 6 cut(s) 120, 123, 307, 403, 705, 708
LweI GCATC 1 cut(s) 450
MaeII ACGT 3 cut(s) 252, 505, 773
MaeIII GTNAC 3 cut(s) 506, 568, 742
MalI GATC 5 cut(s) 39, 193, 449, 521, 642
MbiI CCGCTC 1 cut(s) 337
MboI GATC 5 cut(s) 37, 191, 447, 519, 640
MboII GAAGA 2 cut(s) 385, 397
MflI RGATCY 2 cut(s) 191, 519
MhlI GDGCHC 2 cut(s) 714, 720
MluCI AATT 2 cut(s) 145, 466
MlyI GAGTC 4 cut(s) 191, 227, 541, 550
MmeI TCCRAC 4 cut(s) 188, 225, 264, 612
MroI TCCGGA 1 cut(s) 149
MspI CCGG 7 cut(s) 150, 270, 368, 512, 566, 721, 740
MspR9I CCNGG 3 cut(s) 227, 512, 721
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 1 cut(s) 108
NciI CCSGG 2 cut(s) 512, 721
NdeII GATC 5 cut(s) 37, 191, 447, 519, 640
NlaIII CATG 2 cut(s) 184, 448
NlaIV GGNNCC 1 cut(s) 422
NmeAIII GCCGAG 2 cut(s) 679, 777
NmuCI GTSAC 3 cut(s) 506, 568, 742
PagI TCATGA 2 cut(s) 180, 444
PceI AGGCCT 1 cut(s) 19
PcsI WCGNNNNNNNCGW 3 cut(s) 591, 611, 767
PflFI GACNNNGTC 1 cut(s) 772
PkrI GCNGC 7 cut(s) 66, 110, 113, 297, 393, 695, 698
PleI GAGTC 4 cut(s) 191, 227, 540, 550
PpsI GAGTC 4 cut(s) 191, 227, 540, 550
Psp6I CCWGG 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 568
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 1 cut(s) 422
PsuI RGATCY 2 cut(s) 191, 519
PsyI GACNNNGTC 1 cut(s) 772
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SalI GTCGAC 1 cut(s) 766
SatI GCNGC 7 cut(s) 65, 109, 112, 296, 392, 694, 697
Sau3AI GATC 5 cut(s) 37, 191, 447, 519, 640
SchI GAGTC 4 cut(s) 191, 227, 541, 550
ScrFI CCNGG 3 cut(s) 227, 512, 721
SduI GDGCHC 2 cut(s) 714, 720
SetI ASST 8 cut(s) 180, 221, 255, 408, 432, 488, 508, 776
SfaNI GCATC 1 cut(s) 450
SfcI CTRYAG 1 cut(s) 648
SgrAI CRCCGGYG 1 cut(s) 367
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
Sse9I AATT 2 cut(s) 145, 466
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 5 cut(s) 65, 335, 365, 378, 481
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 3 cut(s) 225, 510, 719
TaiI ACGT 3 cut(s) 255, 508, 776
TaqI TCGA 2 cut(s) 594, 767
TasI AATT 2 cut(s) 145, 466
TatI WGTACW 1 cut(s) 6
TauI GCSGC 1 cut(s) 67
TseFI GTSAC 3 cut(s) 506, 568, 742
TseI GCWGC 6 cut(s) 108, 111, 295, 391, 693, 696
Tsp45I GTSAC 3 cut(s) 506, 568, 742
TspGWI ACGGA 1 cut(s) 537
Tth111I GACNNNGTC 1 cut(s) 772
XmiI GTMKAC 2 cut(s) 767, 776
ZraI GACGTC 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.