Rh5AG229500

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010543
Physical Location & Seq
Reverse (-)
27580587 .. 27581342
756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG229500.1

Sequence Viewer

Length: 756 bp
ATGGAGTACACATCAAGGCCTCAGGAACAAACCCGAGATCATCATCATCATCATCATCAGAAGCCGCCTGCTTCATATTCTCCTCTAAACTCCCCCACCAGCAATGGCAGCAGCAACAACAACAACAACTCCGGAGTTCCAACTCCAACAACTCCAAAGCTGACTCCCAGATCTGACTCCAACCCTTACGCCACTACCTTCGTCCAGGCCGACTCCTCCAACTTCAAGCACGTAGTCCAAATGCTCACCGGCTCATCGGAGACCACAACGAAGCAAACTAACAAGCACACACAGGAGTCATCGTCGTCCCCACTGTCGTCCAAGAACTTCACCATCCCTCCGGTGAAGACCGCGCCGAAGAAGCAGCAGGGCTTCAAGCTCTACGAGAGGAGGAGCCAGAACCTCAAGAACAGCCTGATGATCAATACCCTGATGCCCAATTTTGCCCACCACCGGAGCCAAGCCTCTTCTGGGTTCTCGCCGCGTCACCCGGCCGAGATCTTGTCGCCGAGTCTGCTCGACTTTCCGTCGCTGACGCTGAGCCCGGTAACCCCATTGAACGACGACCATTTTGACAAGTCGGCGTCGTCGCCGTTGGGGAACTCGTCGTCGGAGGAGGATCGGGCTATAGCCGAGAAGGGTTTTTACTTGCACCCGTCTCCGAGAACGAGCACCAGTACTCCCAGAGAGCCAGAGCCCCGGCTTTTGCCATTGTTTCCTGTCACTTCGCCGAGAGTTTCAGGGTCGTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

251

Amino Acids

27.58

Weight (kDa)

9.58

Isoelectric Point (pI)

66.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 62 - 88 2.7e-09 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 353, 484
AccIII TCCGGA 1 cut(s) 131
AciI CCGC 3 cut(s) 65, 351, 482
AclWI GGATC 1 cut(s) 627
AcoI YGGCCR 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 584
AfaI GTAC 2 cut(s) 8, 679
AfiI CCNNNNNNNGG 2 cut(s) 453, 471
AgsI TTSAA 3 cut(s) 226, 376, 559
AhdI GACNNNNNGTC 1 cut(s) 526
AjnI CCWGG 1 cut(s) 204
AluBI AGCT 2 cut(s) 160, 379
AluI AGCT 2 cut(s) 160, 379
Alw21I GWGCWC 1 cut(s) 674
Alw26I GTCTC 2 cut(s) 254, 663
AlwI GGATC 1 cut(s) 627
Ama87I CYCGRG 1 cut(s) 33
Aor13HI TCCGGA 1 cut(s) 131
AoxI GGCC 3 cut(s) 17, 207, 492
ApeKI GCWGC 3 cut(s) 108, 111, 364
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
AspLEI GCGC 1 cut(s) 355
AsuC2I CCSGG 3 cut(s) 491, 545, 700
AsuHPI GGTGA 4 cut(s) 238, 322, 355, 479
AvaI CYCGRG 1 cut(s) 33
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 2 cut(s) 545, 699
BarI GAAGNNNNNNTAC 2 cut(s) 629, 661
BbsI GAAGAC 2 cut(s) 353, 741
Bbv12I GWGCWC 1 cut(s) 674
BbvI GCAGC 3 cut(s) 120, 123, 376
BccI CCATC 1 cut(s) 341
BceAI ACGGC 1 cut(s) 577
BciT130I CCWGG 1 cut(s) 206
BclI TGATCA 1 cut(s) 420
BcnI CCSGG 3 cut(s) 491, 545, 700
BcoDI GTCTC 2 cut(s) 254, 663
BfmI CTRYAG 1 cut(s) 627
BglII AGATCT 2 cut(s) 170, 498
BisI GCNGC 5 cut(s) 65, 109, 112, 365, 482
BlpI GCTNAGC 1 cut(s) 539
BlsI GCNGC 5 cut(s) 66, 110, 113, 366, 483
BmcAI AGTACT 1 cut(s) 679
Bme1390I CCNGG 4 cut(s) 206, 491, 545, 700
BmeRI GACNNNNNGTC 1 cut(s) 526
BmeT110I CYCGRG 1 cut(s) 33
BmiI GGNNCC 2 cut(s) 395, 458
BmrFI CCNGG 4 cut(s) 206, 491, 545, 700
BmsI GCATC 1 cut(s) 423
BpiI GAAGAC 2 cut(s) 353, 741
Bpu1102I GCTNAGC 1 cut(s) 539
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 3 cut(s) 491, 545, 700
BsaAI YACGTR 1 cut(s) 232
BsaBI GATNNNNATC 1 cut(s) 42
BsaHI GRCGYC 1 cut(s) 584
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 698
BsaWI WCCGGW 3 cut(s) 131, 340, 453
BsaXI ACNNNNNCTCC 8 cut(s) 113, 143, 287, 317, 322, 352, 664, 694
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 471
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 1 cut(s) 675
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 42
BseAI TCCGGA 1 cut(s) 131
BseBI CCWGG 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 698
BseGI GGATG 1 cut(s) 333
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 453, 471
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 35, 530
BseNI ACTGG 1 cut(s) 675
BseRI GAGGAG 5 cut(s) 72, 205, 403, 406, 629
BseX3I CGGCCG 1 cut(s) 492
BseXI GCAGC 3 cut(s) 120, 123, 376
Bsh1236I CGCG 2 cut(s) 353, 484
Bsh1285I CGRYCG 1 cut(s) 495
BshFI GGCC 3 cut(s) 19, 209, 494
BsiEI CGRYCG 1 cut(s) 495
BsiHKAI GWGCWC 1 cut(s) 674
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 7 cut(s) 132, 249, 341, 454, 491, 545, 700
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 2 cut(s) 453, 471
BsmAI GTCTC 2 cut(s) 254, 663
BsmBI CGTCTC 1 cut(s) 663
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 3 cut(s) 19, 209, 494
Bso31I GGTCTC 1 cut(s) 254
BsoBI CYCGRG 1 cut(s) 33
Bsp1286I GDGCHC 3 cut(s) 545, 674, 699
Bsp13I TCCGGA 1 cut(s) 131
Bsp143I GATC 5 cut(s) 37, 170, 420, 498, 619
Bsp1720I GCTNAGC 1 cut(s) 539
BspACI CCGC 3 cut(s) 65, 351, 482
BspANI GGCC 3 cut(s) 19, 209, 494
BspCNI CTCAG 2 cut(s) 34, 531
BspEI TCCGGA 1 cut(s) 131
BspFNI CGCG 2 cut(s) 353, 484
BspLI GGNNCC 2 cut(s) 395, 458
BspPI GGATC 1 cut(s) 627
BspTNI GGTCTC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 1 cut(s) 675
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 1 cut(s) 698
BssMI GATC 5 cut(s) 37, 170, 420, 498, 619
BssNI GRCGYC 1 cut(s) 584
Bst2UI CCWGG 1 cut(s) 206
Bst4CI ACNGT 1 cut(s) 315
Bst6I CTCTTC 1 cut(s) 472
BstACI GRCGYC 1 cut(s) 584
BstBAI YACGTR 1 cut(s) 232
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 2 cut(s) 21, 539
BstEII GGTNACC 1 cut(s) 547
BstF5I GGATG 1 cut(s) 333
BstFNI CGCG 2 cut(s) 353, 484
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 5 cut(s) 40, 173, 423, 501, 622
BstMAI GTCTC 2 cut(s) 254, 663
BstMBI GATC 5 cut(s) 37, 170, 420, 498, 619
BstMCI CGRYCG 1 cut(s) 495
BstMWI GCNNNNNNNGC 3 cut(s) 108, 361, 514
BstNI CCWGG 1 cut(s) 206
BstPI GGTNACC 1 cut(s) 547
BstSCI CCNGG 4 cut(s) 204, 489, 543, 698
BstSFI CTRYAG 1 cut(s) 627
BstUI CGCG 2 cut(s) 353, 484
BstV1I GCAGC 3 cut(s) 120, 123, 376
BstV2I GAAGAC 2 cut(s) 353, 741
BstX2I RGATCY 2 cut(s) 170, 498
BstYI RGATCY 2 cut(s) 170, 498
BstZI CGGCCG 1 cut(s) 492
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 3 cut(s) 19, 209, 494
BtsCI GGATG 1 cut(s) 333
BtsIMutI CAGTG 1 cut(s) 311
Cac8I GCNNGC 1 cut(s) 69
CfoI GCGC 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 248
CseI GACGC 3 cut(s) 473, 544, 573
Csp6I GTAC 2 cut(s) 7, 678
CviQI GTAC 2 cut(s) 7, 678
DdeI CTNAG 2 cut(s) 21, 539
DpnI GATC 5 cut(s) 39, 172, 422, 500, 621
DpnII GATC 5 cut(s) 37, 170, 420, 498, 619
DriI GACNNNNNGTC 1 cut(s) 526
EaeI YGGCCR 1 cut(s) 492
EagI CGGCCG 1 cut(s) 492
Eam1104I CTCTTC 1 cut(s) 472
Eam1105I GACNNNNNGTC 1 cut(s) 526
EarI CTCTTC 1 cut(s) 472
EclXI CGGCCG 1 cut(s) 492
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 2 cut(s) 545, 699
Eco31I GGTCTC 1 cut(s) 254
Eco52I CGGCCG 1 cut(s) 492
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 33
Eco91I GGTNACC 1 cut(s) 547
EcoO65I GGTNACC 1 cut(s) 547
EcoRII CCWGG 1 cut(s) 204
EcoT38I GRGCYC 2 cut(s) 545, 699
Esp3I CGTCTC 1 cut(s) 663
FaiI YATR 2 cut(s) 76, 629
FaqI GGGAC 1 cut(s) 292
FbaI TGATCA 1 cut(s) 420
Fnu4HI GCNGC 5 cut(s) 65, 109, 112, 365, 482
FokI GGATG 1 cut(s) 320
FriOI GRGCYC 2 cut(s) 545, 699
Fsp4HI GCNGC 5 cut(s) 65, 109, 112, 365, 482
GlaI GCGC 1 cut(s) 354
GluI GCNGC 5 cut(s) 65, 109, 112, 365, 482
HaeIII GGCC 3 cut(s) 19, 209, 494
HapII CCGG 7 cut(s) 132, 249, 341, 454, 491, 545, 700
HgaI GACGC 3 cut(s) 473, 544, 573
HhaI GCGC 1 cut(s) 355
Hin1I GRCGYC 1 cut(s) 584
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HinfI GANTC 5 cut(s) 163, 176, 212, 296, 511
HpaII CCGG 7 cut(s) 132, 249, 341, 454, 491, 545, 700
HphI GGTGA 4 cut(s) 238, 322, 355, 479
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 5 cut(s) 60, 175, 259, 613, 663
Hpy188III TCNNGA 4 cut(s) 23, 132, 406, 753
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 7 cut(s) 307, 532, 566, 589, 592, 610, 613
HpyAV CCTTC 2 cut(s) 208, 631
HpyCH4III ACNGT 1 cut(s) 315
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 1 cut(s) 652
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 361, 514
HpyF3I CTNAG 2 cut(s) 21, 539
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 1 cut(s) 584
HspAI GCGC 1 cut(s) 353
Kpn2I TCCGGA 1 cut(s) 131
Ksp22I TGATCA 1 cut(s) 420
Kzo9I GATC 5 cut(s) 37, 170, 420, 498, 619
LmnI GCTCC 2 cut(s) 393, 456
Lsp1109I GCAGC 3 cut(s) 120, 123, 376
LweI GCATC 1 cut(s) 423
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 3 cut(s) 485, 547, 721
MalI GATC 5 cut(s) 39, 172, 422, 500, 621
MboI GATC 5 cut(s) 37, 170, 420, 498, 619
MboII GAAGA 4 cut(s) 358, 370, 459, 741
MflI RGATCY 2 cut(s) 170, 498
MhlI GDGCHC 3 cut(s) 545, 674, 699
MluCI AATT 1 cut(s) 439
MlyI GAGTC 5 cut(s) 157, 170, 206, 305, 520
MmeI TCCRAC 5 cut(s) 164, 170, 204, 243, 591
MroI TCCGGA 1 cut(s) 131
MspI CCGG 7 cut(s) 132, 249, 341, 454, 491, 545, 700
MspR9I CCNGG 4 cut(s) 206, 491, 545, 700
MvaI CCWGG 1 cut(s) 206
MvnI CGCG 2 cut(s) 353, 484
MwoI GCNNNNNNNGC 3 cut(s) 108, 361, 514
NciI CCSGG 3 cut(s) 491, 545, 700
NdeII GATC 5 cut(s) 37, 170, 420, 498, 619
NlaIV GGNNCC 2 cut(s) 395, 458
NmeAIII GCCGAG 4 cut(s) 520, 534, 658, 756
NmuCI GTSAC 2 cut(s) 485, 721
PceI AGGCCT 1 cut(s) 19
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PkrI GCNGC 5 cut(s) 66, 110, 113, 366, 483
PleI GAGTC 5 cut(s) 157, 170, 206, 304, 519
PpsI GAGTC 5 cut(s) 157, 170, 206, 304, 519
Ppu21I YACGTR 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 204
PspEI GGTNACC 1 cut(s) 547
PspGI CCWGG 1 cut(s) 204
PspN4I GGNNCC 2 cut(s) 395, 458
PsuI RGATCY 2 cut(s) 170, 498
RsaI GTAC 2 cut(s) 8, 679
RsaNI GTAC 2 cut(s) 7, 678
SatI GCNGC 5 cut(s) 65, 109, 112, 365, 482
Sau3AI GATC 5 cut(s) 37, 170, 420, 498, 619
ScaI AGTACT 1 cut(s) 679
SchI GAGTC 5 cut(s) 157, 170, 206, 305, 520
ScrFI CCNGG 4 cut(s) 206, 491, 545, 700
SduI GDGCHC 3 cut(s) 545, 674, 699
SetI ASST 5 cut(s) 162, 200, 234, 381, 405
SfaNI GCATC 1 cut(s) 423
SfcI CTRYAG 1 cut(s) 627
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 1 cut(s) 439
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 3 cut(s) 65, 351, 482
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 4 cut(s) 204, 489, 543, 698
TaaI ACNGT 1 cut(s) 315
TaiI ACGT 1 cut(s) 234
TaqI TCGA 1 cut(s) 519
TasI AATT 1 cut(s) 439
TatI WGTACW 2 cut(s) 6, 677
TauI GCSGC 2 cut(s) 67, 484
TscAI CASTG 1 cut(s) 318
TseFI GTSAC 2 cut(s) 485, 721
TseI GCWGC 3 cut(s) 108, 111, 364
Tsp45I GTSAC 2 cut(s) 485, 721
TspDTI ATGAA 1 cut(s) 63
TspGWI ACGGA 1 cut(s) 516
TspRI CASTG 1 cut(s) 318
XcmI CCANNNNNNNNNTGG 1 cut(s) 467
ZrmI AGTACT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.