MD03G1229000.v1.1

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010543
Physical Location & Seq
Forward (+)
31457696 .. 31458490
795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1229000.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGAATACACCACAAGCTCAAGGCCTCAAGAAATCAGAGAGAAGCCGTATTCGTATTCCCCCATAAACTCACCGAGGAGCAACAACAATGGCACGACCACCAACGCCAACAGCAGCGTCTGCAGCAGCAGCAGCCCGGCGGTGGGGACTCAAATCCCGACAACCCCGAAAGTCTCAACTCCCAGATCTGACTCCAATCCCTACCCGACAACCTTTGTCCAAGCTGACTCCTCGAACTTCAAACATGTTGTCCAAATGCTCACCGGCTCCTCGGAAACTGTCACTCACCAGTCCTCCCCCAAACCCACAGCCGCCGCCCACCACAACAGCCACCACCACCACCACCACCACCAAGATCCAACAATTTTGCCACCCTCCAATAAAAACTTCAACATCCCACCAATCAAAACCGCCCCACCGAAAAAGCAGGGCTTCAAGCTCTACGAGCGGCGGAGCACCAATCTCAAAAACACCCTCATGATCAACACCCTCATCCCCAACTTCCAATCGGCTTCTGGGTTTTCTCCACGTCATCAGGAGATTCTCTCACCGAGCCTACTCGAATTCCCGTCACTCACCCTCAGCCCGGTGACGCCGTTGAACGACCAGTTCGACAAGTCGGCGTCGTCGCCGTCACTGGGAAATTCTTCGTCGGAGGAGGACAGGGCGATTGCGGAGAAAGGATTTTACTTGCACCCGTCGCCGAGGGCTACGACGACTCCGAGAGACCCGGAGCCGCGGCTTCTGCCTCTGTTTCCGGTCACTTCGCCGAGAGTTTCTGGGTCGTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

265

Amino Acids

28.84

Weight (kDa)

9.49

Isoelectric Point (pI)

64.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 67 - 93 3.4e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 448
AccII CGCG 1 cut(s) 739
AciI CCGC 9 cut(s) 140, 312, 315, 411, 448, 451, 674, 737, 739
AclWI GGATC 1 cut(s) 350
AcsI RAATTY 2 cut(s) 563, 643
AcyI GRCGYC 2 cut(s) 593, 623
AfiI CCNNNNNNNGG 4 cut(s) 142, 143, 586, 638
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 4 cut(s) 241, 391, 436, 601
AjiI CACGTC 1 cut(s) 530
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 3 cut(s) 18, 224, 439
AluI AGCT 3 cut(s) 18, 224, 439
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 2 cut(s) 178, 720
AlwI GGATC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 120
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 5 cut(s) 114, 123, 126, 129, 132
ApoI RAATTY 2 cut(s) 563, 643
ArsI GACNNNNNNTTYG 2 cut(s) 234, 266
Asp700I GAANNNNTTC 1 cut(s) 646
AsuC2I CCSGG 3 cut(s) 137, 587, 731
AsuHPI GGTGA 6 cut(s) 63, 253, 278, 540, 568, 601
BbsI GAAGAC 1 cut(s) 780
Bbv12I GWGCWC 1 cut(s) 458
BbvCI CCTCAGC 1 cut(s) 581
BbvI GCAGC 5 cut(s) 126, 135, 138, 141, 144
BceAI ACGGC 3 cut(s) 31, 580, 616
BclI TGATCA 1 cut(s) 480
BcnI CCSGG 3 cut(s) 137, 587, 731
BcoDI GTCTC 2 cut(s) 178, 720
BfmI CTRYAG 1 cut(s) 121
BglII AGATCT 1 cut(s) 185
Bme1390I CCNGG 3 cut(s) 137, 587, 731
BmgBI CACGTC 1 cut(s) 530
BmiI GGNNCC 2 cut(s) 268, 735
BmrFI CCNGG 3 cut(s) 137, 587, 731
BmrI ACTGGG 1 cut(s) 647
BmuI ACTGGG 1 cut(s) 647
BpiI GAAGAC 1 cut(s) 780
BplI GAGNNNNNCTC 2 cut(s) 530, 562
Bpu10I CCTNAGC 1 cut(s) 581
BpuEI CTTGAG 2 cut(s) 4, 12
BpuMI CCSGG 3 cut(s) 137, 587, 731
BsaHI GRCGYC 2 cut(s) 593, 623
BsaI GGTCTC 1 cut(s) 720
BsaJI CCNNGG 4 cut(s) 74, 270, 704, 737
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 703, 733
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 143, 586, 638
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 289, 607, 642
BseDI CCNNGG 4 cut(s) 74, 270, 704, 737
BseGI GGATG 2 cut(s) 393, 492
BseLI CCNNNNNNNGG 4 cut(s) 142, 143, 586, 638
BseMII CTCAG 1 cut(s) 595
BseNI ACTGG 3 cut(s) 289, 607, 642
BseRI GAGGAG 4 cut(s) 91, 220, 259, 671
BseXI GCAGC 5 cut(s) 126, 135, 138, 141, 144
Bsh1236I CGCG 1 cut(s) 739
BshFI GGCC 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 458
BsiSI CCGG 5 cut(s) 137, 264, 587, 731, 758
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 4 cut(s) 142, 143, 586, 638
BsmAI GTCTC 2 cut(s) 178, 720
BsmFI GGGAC 1 cut(s) 160
BsnI GGCC 1 cut(s) 25
Bso31I GGTCTC 1 cut(s) 720
Bsp1286I GDGCHC 1 cut(s) 458
Bsp143I GATC 3 cut(s) 185, 355, 480
BspACI CCGC 9 cut(s) 140, 312, 315, 411, 448, 451, 674, 737, 739
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 1 cut(s) 594
BspFNI CGCG 1 cut(s) 739
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 2 cut(s) 268, 735
BspMAI CTGCAG 1 cut(s) 125
BspPI GGATC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 720
BsrBI CCGCTC 1 cut(s) 448
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 3 cut(s) 289, 607, 642
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 4 cut(s) 74, 270, 704, 737
BssMI GATC 3 cut(s) 185, 355, 480
BssNI GRCGYC 2 cut(s) 593, 623
Bst4CI ACNGT 1 cut(s) 280
BstACI GRCGYC 2 cut(s) 593, 623
BstAPI GCANNNNNTGC 1 cut(s) 120
BstDEI CTNAG 1 cut(s) 581
BstDSI CCRYGG 1 cut(s) 737
BstF5I GGATG 2 cut(s) 393, 492
BstFNI CGCG 1 cut(s) 739
BstKTI GATC 3 cut(s) 188, 358, 483
BstMAI GTCTC 2 cut(s) 178, 720
BstMBI GATC 3 cut(s) 185, 355, 480
BstMWI GCNNNNNNNGC 7 cut(s) 120, 123, 129, 132, 445, 700, 745
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 3 cut(s) 135, 585, 729
BstSFI CTRYAG 1 cut(s) 121
BstUI CGCG 1 cut(s) 739
BstV1I GCAGC 5 cut(s) 126, 135, 138, 141, 144
BstV2I GAAGAC 1 cut(s) 780
BstX2I RGATCY 2 cut(s) 185, 355
BstYI RGATCY 2 cut(s) 185, 355
BsuRI GGCC 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 737
BtrI CACGTC 1 cut(s) 530
BtsCI GGATG 2 cut(s) 393, 492
BtsIMutI CAGTG 1 cut(s) 635
CaiI CAGNNNCTG 1 cut(s) 120
CciI TCATGA 1 cut(s) 477
Cfr10I RCCGGY 1 cut(s) 263
Cfr42I CCGCGG 1 cut(s) 740
CseI GACGC 3 cut(s) 106, 601, 612
CviAII CATG 2 cut(s) 245, 478
DdeI CTNAG 1 cut(s) 581
DpnI GATC 3 cut(s) 187, 357, 482
DpnII GATC 3 cut(s) 185, 355, 480
EciI GGCGGA 1 cut(s) 466
Eco147I AGGCCT 1 cut(s) 25
Eco31I GGTCTC 1 cut(s) 720
EcoRI GAATTC 1 cut(s) 563
FaeI CATG 2 cut(s) 248, 481
FaiI YATR 3 cut(s) 65, 246, 479
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 1 cut(s) 160
FatI CATG 2 cut(s) 244, 477
FbaI TGATCA 1 cut(s) 480
FokI GGATG 2 cut(s) 380, 479
HaeIII GGCC 1 cut(s) 25
HapII CCGG 5 cut(s) 137, 264, 587, 731, 758
HgaI GACGC 3 cut(s) 106, 601, 612
Hin1I GRCGYC 2 cut(s) 593, 623
Hin1II CATG 2 cut(s) 248, 481
HinfI GANTC 5 cut(s) 148, 191, 227, 541, 718
HpaII CCGG 5 cut(s) 137, 264, 587, 731, 758
HphI GGTGA 6 cut(s) 63, 253, 278, 540, 568, 601
Hpy188I TCNGA 5 cut(s) 38, 190, 274, 655, 723
Hpy188III TCNNGA 5 cut(s) 29, 157, 478, 536, 792
Hpy99I CGWCG 5 cut(s) 628, 631, 655, 703, 718
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 529
HpyCH4V TGCA 2 cut(s) 123, 694
HpyF10VI GCNNNNNNNGC 7 cut(s) 120, 123, 129, 132, 445, 700, 745
HpyF3I CTNAG 1 cut(s) 581
HpySE526I ACGT 1 cut(s) 529
Hsp92I GRCGYC 2 cut(s) 593, 623
Hsp92II CATG 2 cut(s) 248, 481
Ksp22I TGATCA 1 cut(s) 480
KspI CCGCGG 1 cut(s) 740
Kzo9I GATC 3 cut(s) 185, 355, 480
LmnI GCTCC 4 cut(s) 78, 272, 453, 733
Lsp1109I GCAGC 5 cut(s) 126, 135, 138, 141, 144
MaeII ACGT 1 cut(s) 529
MaeIII GTNAC 5 cut(s) 280, 570, 589, 633, 760
MalI GATC 3 cut(s) 187, 357, 482
MbiI CCGCTC 1 cut(s) 448
MboI GATC 3 cut(s) 185, 355, 480
MboII GAAGA 2 cut(s) 639, 780
MflI RGATCY 2 cut(s) 185, 355
MhlI GDGCHC 1 cut(s) 458
MluCI AATT 3 cut(s) 363, 563, 643
MlyI GAGTC 4 cut(s) 142, 185, 221, 712
MmeI TCCRAC 2 cut(s) 383, 633
MroXI GAANNNNTTC 1 cut(s) 646
MslI CAYNNNNRTG 1 cut(s) 476
MspA1I CMGCKG 1 cut(s) 739
MspI CCGG 5 cut(s) 137, 264, 587, 731, 758
MspR9I CCNGG 3 cut(s) 137, 587, 731
MvnI CGCG 1 cut(s) 739
MwoI GCNNNNNNNGC 7 cut(s) 120, 123, 129, 132, 445, 700, 745
NciI CCSGG 3 cut(s) 137, 587, 731
NdeII GATC 3 cut(s) 185, 355, 480
NlaIII CATG 2 cut(s) 248, 481
NlaIV GGNNCC 2 cut(s) 268, 735
NmeAIII GCCGAG 2 cut(s) 729, 795
NmuCI GTSAC 5 cut(s) 280, 570, 589, 633, 760
NspI RCATGY 1 cut(s) 248
PagI TCATGA 1 cut(s) 477
PceI AGGCCT 1 cut(s) 25
PciI ACATGT 1 cut(s) 244
PcsI WCGNNNNNNNCGW 2 cut(s) 609, 719
PdmI GAANNNNTTC 1 cut(s) 646
PfeI GAWTC 1 cut(s) 541
PleI GAGTC 4 cut(s) 142, 185, 221, 712
PpsI GAGTC 4 cut(s) 142, 185, 221, 712
PscI ACATGT 1 cut(s) 244
PspN4I GGNNCC 2 cut(s) 268, 735
PstI CTGCAG 1 cut(s) 125
PstNI CAGNNNCTG 1 cut(s) 120
PsuI RGATCY 2 cut(s) 185, 355
RseI CAYNNNNRTG 1 cut(s) 476
SacII CCGCGG 1 cut(s) 740
Sau3AI GATC 3 cut(s) 185, 355, 480
SchI GAGTC 4 cut(s) 142, 185, 221, 712
ScrFI CCNGG 3 cut(s) 137, 587, 731
SduI GDGCHC 1 cut(s) 458
SetI ASST 5 cut(s) 20, 215, 226, 441, 532
SfcI CTRYAG 1 cut(s) 121
Sfr303I CCGCGG 1 cut(s) 740
SgrBI CCGCGG 1 cut(s) 740
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 2 cut(s) 19, 27
SmoI CTYRAG 2 cut(s) 19, 27
Sse9I AATT 3 cut(s) 363, 563, 643
SseBI AGGCCT 1 cut(s) 25
SsiI CCGC 9 cut(s) 140, 312, 315, 411, 448, 451, 674, 737, 739
StuI AGGCCT 1 cut(s) 25
StyD4I CCNGG 3 cut(s) 135, 585, 729
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 1 cut(s) 532
TaqI TCGA 3 cut(s) 233, 561, 612
TasI AATT 3 cut(s) 363, 563, 643
TauI GCSGC 5 cut(s) 314, 317, 451, 739, 742
TfiI GAWTC 1 cut(s) 541
TscAI CASTG 1 cut(s) 642
TseFI GTSAC 5 cut(s) 280, 570, 589, 633, 760
TseI GCWGC 5 cut(s) 114, 123, 126, 129, 132
Tsp45I GTSAC 5 cut(s) 280, 570, 589, 633, 760
TspRI CASTG 1 cut(s) 642
XapI RAATTY 2 cut(s) 563, 643
XceI RCATGY 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 512
XmnI GAANNNNTTC 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.