Rmu_ssc0000299.1_g000004

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000299.1
OG0010543
Physical Location & Seq
Reverse (-)
24612 .. 25370
759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000299.1_g000004.1.cds

Sequence Viewer

Length: 759 bp
atggagtacacatcaaggcctcaggaacaaacccgagatcatcatcatcatcatcatcagaagccgcctgcttcatattctcctctaaactcccccaccagcaatggcagcagcagcaacaacaacaacaactccggagttcaaactccgacaactccaaagctgactcccagatctgactccaacccttacgccaccaccttcgtccaggccgactcctccaacttcaagcacgtagtccaaatgctcaccggctcatcggagaccacaacgaagcaaactaacaagcacacacaggagtcatcgtcgtccccactgtcgtccaagaacttcgccatccctccggtgaagaccgcgccgaagaagcagcagggcttcaagctctacgagaggaggagccagaacctcaagaacagcctgatgatcaataccctgatgcccaattttgcccaccaccggagccaagcctcttctgggttctcgccgcgtcacccggccgagatcttgtcgccgagcctgctcgactttccgtcgctgacgctgagcccggtgaccccattgaacgacgaccattttgacaagtcggcgtcgtcgccgttggggaactcgtcgtcggaggaggatcgggctatagccgagaagggtttttacttgcacccgtctccgagaacgagcaccagcactcccagagagccagagccccggcttttgccattgtttccggtcacttcgccgagagtttcagggtcgtcttcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

252

Amino Acids

27.67

Weight (kDa)

9.58

Isoelectric Point (pI)

66.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 356, 487
AccIII TCCGGA 1 cut(s) 134
AciI CCGC 3 cut(s) 65, 354, 485
AclWI GGATC 1 cut(s) 630
AcoI YGGCCR 1 cut(s) 495
AcyI GRCGYC 1 cut(s) 587
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 2 cut(s) 456, 474
AgsI TTSAA 4 cut(s) 143, 229, 379, 562
AhdI GACNNNNNGTC 1 cut(s) 529
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 2 cut(s) 163, 382
AluI AGCT 2 cut(s) 163, 382
Alw21I GWGCWC 1 cut(s) 677
Alw26I GTCTC 2 cut(s) 257, 666
AlwI GGATC 1 cut(s) 630
Ama87I CYCGRG 1 cut(s) 33
Aor13HI TCCGGA 1 cut(s) 134
AoxI GGCC 3 cut(s) 17, 210, 495
ApeKI GCWGC 4 cut(s) 108, 111, 114, 367
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
AspLEI GCGC 1 cut(s) 358
AsuC2I CCSGG 3 cut(s) 494, 548, 703
AsuHPI GGTGA 4 cut(s) 241, 358, 482, 562
AvaI CYCGRG 1 cut(s) 33
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 2 cut(s) 548, 702
BarI GAAGNNNNNNTAC 2 cut(s) 632, 664
BbsI GAAGAC 2 cut(s) 356, 744
Bbv12I GWGCWC 1 cut(s) 677
BbvI GCAGC 4 cut(s) 120, 123, 126, 379
BccI CCATC 1 cut(s) 344
BceAI ACGGC 1 cut(s) 580
BciT130I CCWGG 1 cut(s) 209
BclI TGATCA 1 cut(s) 423
BcnI CCSGG 3 cut(s) 494, 548, 703
BcoDI GTCTC 2 cut(s) 257, 666
BfmI CTRYAG 1 cut(s) 630
BglII AGATCT 2 cut(s) 173, 501
BisI GCNGC 6 cut(s) 65, 109, 112, 115, 368, 485
BlpI GCTNAGC 1 cut(s) 542
BlsI GCNGC 6 cut(s) 66, 110, 113, 116, 369, 486
Bme1390I CCNGG 4 cut(s) 209, 494, 548, 703
BmeRI GACNNNNNGTC 1 cut(s) 529
BmeT110I CYCGRG 1 cut(s) 33
BmiI GGNNCC 2 cut(s) 398, 461
BmrFI CCNGG 4 cut(s) 209, 494, 548, 703
BmsI GCATC 1 cut(s) 426
BpiI GAAGAC 2 cut(s) 356, 744
Bpu1102I GCTNAGC 1 cut(s) 542
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 3 cut(s) 494, 548, 703
BsaAI YACGTR 1 cut(s) 235
BsaBI GATNNNNATC 1 cut(s) 42
BsaHI GRCGYC 1 cut(s) 587
BsaI GGTCTC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 701
BsaWI WCCGGW 4 cut(s) 134, 343, 456, 721
BsaXI ACNNNNNCTCC 6 cut(s) 116, 146, 290, 320, 667, 697
Bsc4I CCNNNNNNNGG 2 cut(s) 456, 474
Bse118I RCCGGY 1 cut(s) 251
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 42
BseAI TCCGGA 1 cut(s) 134
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 1 cut(s) 701
BseGI GGATG 1 cut(s) 336
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 456, 474
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 35, 533
BseRI GAGGAG 5 cut(s) 72, 208, 406, 409, 632
BseX3I CGGCCG 1 cut(s) 495
BseXI GCAGC 4 cut(s) 120, 123, 126, 379
Bsh1236I CGCG 2 cut(s) 356, 487
Bsh1285I CGRYCG 1 cut(s) 498
BshFI GGCC 3 cut(s) 19, 212, 497
BsiEI CGRYCG 1 cut(s) 498
BsiHKAI GWGCWC 1 cut(s) 677
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 8 cut(s) 135, 252, 344, 457, 494, 548, 703, 722
BslFI GGGAC 1 cut(s) 295
BslI CCNNNNNNNGG 2 cut(s) 456, 474
BsmAI GTCTC 2 cut(s) 257, 666
BsmBI CGTCTC 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 295
BsnI GGCC 3 cut(s) 19, 212, 497
Bso31I GGTCTC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 33
Bsp1286I GDGCHC 3 cut(s) 548, 677, 702
Bsp13I TCCGGA 1 cut(s) 134
Bsp143I GATC 5 cut(s) 37, 173, 423, 501, 622
Bsp1720I GCTNAGC 1 cut(s) 542
BspACI CCGC 3 cut(s) 65, 354, 485
BspANI GGCC 3 cut(s) 19, 212, 497
BspCNI CTCAG 2 cut(s) 34, 534
BspEI TCCGGA 1 cut(s) 134
BspFNI CGCG 2 cut(s) 356, 487
BspLI GGNNCC 2 cut(s) 398, 461
BspPI GGATC 1 cut(s) 630
BspTNI GGTCTC 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 251
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 1 cut(s) 701
BssMI GATC 5 cut(s) 37, 173, 423, 501, 622
BssNI GRCGYC 1 cut(s) 587
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 318
Bst6I CTCTTC 1 cut(s) 475
BstACI GRCGYC 1 cut(s) 587
BstBAI YACGTR 1 cut(s) 235
BstC8I GCNNGC 2 cut(s) 69, 518
BstDEI CTNAG 2 cut(s) 21, 542
BstEII GGTNACC 1 cut(s) 550
BstF5I GGATG 1 cut(s) 336
BstFNI CGCG 2 cut(s) 356, 487
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 5 cut(s) 40, 176, 426, 504, 625
BstMAI GTCTC 2 cut(s) 257, 666
BstMBI GATC 5 cut(s) 37, 173, 423, 501, 622
BstMCI CGRYCG 1 cut(s) 498
BstMWI GCNNNNNNNGC 4 cut(s) 108, 114, 364, 517
BstNI CCWGG 1 cut(s) 209
BstPI GGTNACC 1 cut(s) 550
BstSCI CCNGG 4 cut(s) 207, 492, 546, 701
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 2 cut(s) 356, 487
BstV1I GCAGC 4 cut(s) 120, 123, 126, 379
BstV2I GAAGAC 2 cut(s) 356, 744
BstX2I RGATCY 2 cut(s) 173, 501
BstYI RGATCY 2 cut(s) 173, 501
BstZI CGGCCG 1 cut(s) 495
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 3 cut(s) 19, 212, 497
BtsCI GGATG 1 cut(s) 336
BtsIMutI CAGTG 1 cut(s) 314
Cac8I GCNNGC 2 cut(s) 69, 518
CfoI GCGC 1 cut(s) 358
Cfr10I RCCGGY 1 cut(s) 251
CseI GACGC 3 cut(s) 476, 547, 576
Csp6I GTAC 1 cut(s) 7
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 2 cut(s) 21, 542
DpnI GATC 5 cut(s) 39, 175, 425, 503, 624
DpnII GATC 5 cut(s) 37, 173, 423, 501, 622
DriI GACNNNNNGTC 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 495
EagI CGGCCG 1 cut(s) 495
Eam1104I CTCTTC 1 cut(s) 475
Eam1105I GACNNNNNGTC 1 cut(s) 529
EarI CTCTTC 1 cut(s) 475
EclXI CGGCCG 1 cut(s) 495
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 2 cut(s) 548, 702
Eco31I GGTCTC 1 cut(s) 257
Eco52I CGGCCG 1 cut(s) 495
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 33
Eco91I GGTNACC 1 cut(s) 550
EcoO65I GGTNACC 1 cut(s) 550
EcoRII CCWGG 1 cut(s) 207
EcoT38I GRGCYC 2 cut(s) 548, 702
Esp3I CGTCTC 1 cut(s) 666
FaiI YATR 2 cut(s) 76, 632
FaqI GGGAC 1 cut(s) 295
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 6 cut(s) 65, 109, 112, 115, 368, 485
FokI GGATG 1 cut(s) 323
FriOI GRGCYC 2 cut(s) 548, 702
Fsp4HI GCNGC 6 cut(s) 65, 109, 112, 115, 368, 485
GlaI GCGC 1 cut(s) 357
GluI GCNGC 6 cut(s) 65, 109, 112, 115, 368, 485
HaeIII GGCC 3 cut(s) 19, 212, 497
HapII CCGG 8 cut(s) 135, 252, 344, 457, 494, 548, 703, 722
HgaI GACGC 3 cut(s) 476, 547, 576
HhaI GCGC 1 cut(s) 358
Hin1I GRCGYC 1 cut(s) 587
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HinfI GANTC 4 cut(s) 166, 179, 215, 299
HpaII CCGG 8 cut(s) 135, 252, 344, 457, 494, 548, 703, 722
HphI GGTGA 4 cut(s) 241, 358, 482, 562
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 6 cut(s) 60, 150, 178, 262, 616, 666
Hpy188III TCNNGA 4 cut(s) 23, 135, 409, 756
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 7 cut(s) 310, 535, 569, 592, 595, 613, 616
HpyAV CCTTC 2 cut(s) 211, 634
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 1 cut(s) 655
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 114, 364, 517
HpyF3I CTNAG 2 cut(s) 21, 542
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 1 cut(s) 587
HspAI GCGC 1 cut(s) 356
Kpn2I TCCGGA 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 5 cut(s) 37, 173, 423, 501, 622
LmnI GCTCC 2 cut(s) 396, 459
Lsp1109I GCAGC 4 cut(s) 120, 123, 126, 379
LweI GCATC 1 cut(s) 426
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 3 cut(s) 488, 550, 724
MalI GATC 5 cut(s) 39, 175, 425, 503, 624
MboI GATC 5 cut(s) 37, 173, 423, 501, 622
MboII GAAGA 4 cut(s) 361, 373, 462, 744
MflI RGATCY 2 cut(s) 173, 501
MhlI GDGCHC 3 cut(s) 548, 677, 702
MluCI AATT 1 cut(s) 442
MlyI GAGTC 4 cut(s) 160, 173, 209, 308
MmeI TCCRAC 4 cut(s) 173, 207, 246, 594
MroI TCCGGA 1 cut(s) 134
MspI CCGG 8 cut(s) 135, 252, 344, 457, 494, 548, 703, 722
MspR9I CCNGG 4 cut(s) 209, 494, 548, 703
MvaI CCWGG 1 cut(s) 209
MvnI CGCG 2 cut(s) 356, 487
MwoI GCNNNNNNNGC 4 cut(s) 108, 114, 364, 517
NciI CCSGG 3 cut(s) 494, 548, 703
NdeII GATC 5 cut(s) 37, 173, 423, 501, 622
NlaIV GGNNCC 2 cut(s) 398, 461
NmeAIII GCCGAG 4 cut(s) 523, 537, 661, 759
NmuCI GTSAC 3 cut(s) 488, 550, 724
PceI AGGCCT 1 cut(s) 19
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PkrI GCNGC 6 cut(s) 66, 110, 113, 116, 369, 486
PleI GAGTC 4 cut(s) 160, 173, 209, 307
PpsI GAGTC 4 cut(s) 160, 173, 209, 307
Ppu21I YACGTR 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 207
PspEI GGTNACC 1 cut(s) 550
PspGI CCWGG 1 cut(s) 207
PspN4I GGNNCC 2 cut(s) 398, 461
PsuI RGATCY 2 cut(s) 173, 501
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SatI GCNGC 6 cut(s) 65, 109, 112, 115, 368, 485
Sau3AI GATC 5 cut(s) 37, 173, 423, 501, 622
SchI GAGTC 4 cut(s) 160, 173, 209, 308
ScrFI CCNGG 4 cut(s) 209, 494, 548, 703
SduI GDGCHC 3 cut(s) 548, 677, 702
SetI ASST 5 cut(s) 165, 203, 237, 384, 408
SfaNI GCATC 1 cut(s) 426
SfcI CTRYAG 1 cut(s) 630
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 1 cut(s) 442
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 3 cut(s) 65, 354, 485
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 4 cut(s) 207, 492, 546, 701
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 1 cut(s) 237
TaqI TCGA 1 cut(s) 522
TasI AATT 1 cut(s) 442
TatI WGTACW 1 cut(s) 6
TauI GCSGC 2 cut(s) 67, 487
TscAI CASTG 1 cut(s) 321
TseFI GTSAC 3 cut(s) 488, 550, 724
TseI GCWGC 4 cut(s) 108, 111, 114, 367
Tsp45I GTSAC 3 cut(s) 488, 550, 724
TspDTI ATGAA 1 cut(s) 63
TspGWI ACGGA 1 cut(s) 519
TspRI CASTG 1 cut(s) 321
XcmI CCANNNNNNNNNTGG 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.