Rh5BG230500

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010543
Physical Location & Seq
Reverse (-)
27075398 .. 27077460
2063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG230500.1

Sequence Viewer

Length: 762 bp
ATGGAGTACACATCAAGGCCTCAGGAACAAACCCGAGATCATCATCATCATCATCATCATCAGAAGCCGCCTGCTTCATATTCTCCTCTAAACTCCCCCACCAGCAATGGCAGCAGCAGCAACAACAACAACAACTCCGGAGTTCAAACTCCGACAACTCCAAAGCTGACTCCCAGATCTGACTCCAACCCTTACGCCACCACCTTCGTCCAGGCCGACTCCTCCAACTTCAAGCACGTAGTCCAAATGCTCACCGGCTCATCGGAGACCACAACGAAGCAAACTAACAAGCACACACAGGAGTCATCGTCGTCCCCACTGTCGTCCAAGAACTTCACCATCCCTCCGGTGAAGACCGCGCCGAAGAAGCAGCAGGGCTTCAAGCTCTACGAGAGGAGGAGCCAGAACCTCAAGAACAGCCTGATGATCAATACCCTGATGCCCAATTTTGCCCACCACCGGAGCCAAGCCTCTTCTGGGTTCTCGCCGCGTCACCCGGCCGAGATCTTGTCTCCGAGTCTGCTCGACTTTCCGTCGCTGACGCTGAGCCCGGTGACCCCATTGAACGACGACCATTTTGACAAGTCGGCGTCGTCGCCGTTGGGGAACTCGTCGTCGGAGGAGGATCGGGCTATAGCCGAGAAGGGTTTTTACTTGCACCCGTCTCCGAGAACGAGCACCAGCACTCCCAGAGAGCCAGAGCCCCGGCTTTTGCCATTGTTTCCGGTCACTTCGCCGAGAGTTTCAGGGTCGTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

253

Amino Acids

27.83

Weight (kDa)

9.58

Isoelectric Point (pI)

66.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 64 - 90 2.8e-09 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 359, 490
AccIII TCCGGA 1 cut(s) 137
AciI CCGC 3 cut(s) 68, 357, 488
AclWI GGATC 1 cut(s) 633
AcoI YGGCCR 1 cut(s) 498
AcyI GRCGYC 1 cut(s) 590
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 2 cut(s) 459, 477
AgsI TTSAA 4 cut(s) 146, 232, 382, 565
AhdI GACNNNNNGTC 1 cut(s) 532
AjnI CCWGG 1 cut(s) 210
AluBI AGCT 2 cut(s) 166, 385
AluI AGCT 2 cut(s) 166, 385
Alw21I GWGCWC 1 cut(s) 680
Alw26I GTCTC 3 cut(s) 260, 516, 669
AlwI GGATC 1 cut(s) 633
Ama87I CYCGRG 1 cut(s) 33
Aor13HI TCCGGA 1 cut(s) 137
AoxI GGCC 3 cut(s) 17, 213, 498
ApeKI GCWGC 4 cut(s) 111, 114, 117, 370
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
AspLEI GCGC 1 cut(s) 361
AsuC2I CCSGG 3 cut(s) 497, 551, 706
AsuHPI GGTGA 5 cut(s) 244, 328, 361, 485, 565
AvaI CYCGRG 1 cut(s) 33
AxyI CCTNAGG 1 cut(s) 21
BanII GRGCYC 2 cut(s) 551, 705
BarI GAAGNNNNNNTAC 2 cut(s) 635, 667
BbsI GAAGAC 2 cut(s) 359, 747
Bbv12I GWGCWC 1 cut(s) 680
BbvI GCAGC 4 cut(s) 123, 126, 129, 382
BccI CCATC 1 cut(s) 347
BceAI ACGGC 1 cut(s) 583
BciT130I CCWGG 1 cut(s) 212
BclI TGATCA 1 cut(s) 426
BcnI CCSGG 3 cut(s) 497, 551, 706
BcoDI GTCTC 3 cut(s) 260, 516, 669
BfmI CTRYAG 1 cut(s) 633
BglII AGATCT 2 cut(s) 176, 504
BisI GCNGC 6 cut(s) 68, 112, 115, 118, 371, 488
BlpI GCTNAGC 1 cut(s) 545
BlsI GCNGC 6 cut(s) 69, 113, 116, 119, 372, 489
Bme1390I CCNGG 4 cut(s) 212, 497, 551, 706
BmeRI GACNNNNNGTC 1 cut(s) 532
BmeT110I CYCGRG 1 cut(s) 33
BmiI GGNNCC 2 cut(s) 401, 464
BmrFI CCNGG 4 cut(s) 212, 497, 551, 706
BmsI GCATC 1 cut(s) 429
BpiI GAAGAC 2 cut(s) 359, 747
Bpu1102I GCTNAGC 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 3 cut(s) 497, 551, 706
BsaAI YACGTR 1 cut(s) 238
BsaBI GATNNNNATC 1 cut(s) 42
BsaHI GRCGYC 1 cut(s) 590
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 1 cut(s) 704
BsaWI WCCGGW 4 cut(s) 137, 346, 459, 724
BsaXI ACNNNNNCTCC 8 cut(s) 119, 149, 293, 323, 328, 358, 670, 700
Bsc4I CCNNNNNNNGG 2 cut(s) 459, 477
Bse118I RCCGGY 1 cut(s) 254
Bse21I CCTNAGG 1 cut(s) 21
Bse3DI GCAATG 1 cut(s) 112
Bse8I GATNNNNATC 1 cut(s) 42
BseAI TCCGGA 1 cut(s) 137
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 1 cut(s) 704
BseGI GGATG 1 cut(s) 339
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 459, 477
BseMI GCAATG 1 cut(s) 112
BseMII CTCAG 2 cut(s) 35, 536
BseRI GAGGAG 5 cut(s) 75, 211, 409, 412, 635
BseX3I CGGCCG 1 cut(s) 498
BseXI GCAGC 4 cut(s) 123, 126, 129, 382
Bsh1236I CGCG 2 cut(s) 359, 490
Bsh1285I CGRYCG 1 cut(s) 501
BshFI GGCC 3 cut(s) 19, 215, 500
BsiEI CGRYCG 1 cut(s) 501
BsiHKAI GWGCWC 1 cut(s) 680
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 8 cut(s) 138, 255, 347, 460, 497, 551, 706, 725
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 2 cut(s) 459, 477
BsmAI GTCTC 3 cut(s) 260, 516, 669
BsmBI CGTCTC 1 cut(s) 669
BsmFI GGGAC 1 cut(s) 298
BsnI GGCC 3 cut(s) 19, 215, 500
Bso31I GGTCTC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 33
Bsp1286I GDGCHC 3 cut(s) 551, 680, 705
Bsp13I TCCGGA 1 cut(s) 137
Bsp143I GATC 5 cut(s) 37, 176, 426, 504, 625
Bsp1720I GCTNAGC 1 cut(s) 545
BspACI CCGC 3 cut(s) 68, 357, 488
BspANI GGCC 3 cut(s) 19, 215, 500
BspCNI CTCAG 2 cut(s) 34, 537
BspEI TCCGGA 1 cut(s) 137
BspFNI CGCG 2 cut(s) 359, 490
BspLI GGNNCC 2 cut(s) 401, 464
BspPI GGATC 1 cut(s) 633
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 254
BssAI RCCGGY 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 704
BssMI GATC 5 cut(s) 37, 176, 426, 504, 625
BssNI GRCGYC 1 cut(s) 590
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 321
Bst6I CTCTTC 1 cut(s) 478
BstACI GRCGYC 1 cut(s) 590
BstBAI YACGTR 1 cut(s) 238
BstC8I GCNNGC 1 cut(s) 72
BstDEI CTNAG 2 cut(s) 21, 545
BstEII GGTNACC 1 cut(s) 553
BstF5I GGATG 1 cut(s) 339
BstFNI CGCG 2 cut(s) 359, 490
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 5 cut(s) 40, 179, 429, 507, 628
BstMAI GTCTC 3 cut(s) 260, 516, 669
BstMBI GATC 5 cut(s) 37, 176, 426, 504, 625
BstMCI CGRYCG 1 cut(s) 501
BstMWI GCNNNNNNNGC 3 cut(s) 111, 117, 367
BstNI CCWGG 1 cut(s) 212
BstPI GGTNACC 1 cut(s) 553
BstSCI CCNGG 4 cut(s) 210, 495, 549, 704
BstSFI CTRYAG 1 cut(s) 633
BstUI CGCG 2 cut(s) 359, 490
BstV1I GCAGC 4 cut(s) 123, 126, 129, 382
BstV2I GAAGAC 2 cut(s) 359, 747
BstX2I RGATCY 2 cut(s) 176, 504
BstYI RGATCY 2 cut(s) 176, 504
BstZI CGGCCG 1 cut(s) 498
Bsu36I CCTNAGG 1 cut(s) 21
BsuRI GGCC 3 cut(s) 19, 215, 500
BtsCI GGATG 1 cut(s) 339
BtsIMutI CAGTG 1 cut(s) 317
Cac8I GCNNGC 1 cut(s) 72
CfoI GCGC 1 cut(s) 361
Cfr10I RCCGGY 1 cut(s) 254
CseI GACGC 3 cut(s) 479, 550, 579
Csp6I GTAC 1 cut(s) 7
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 2 cut(s) 21, 545
DpnI GATC 5 cut(s) 39, 178, 428, 506, 627
DpnII GATC 5 cut(s) 37, 176, 426, 504, 625
DriI GACNNNNNGTC 1 cut(s) 532
EaeI YGGCCR 1 cut(s) 498
EagI CGGCCG 1 cut(s) 498
Eam1104I CTCTTC 1 cut(s) 478
Eam1105I GACNNNNNGTC 1 cut(s) 532
EarI CTCTTC 1 cut(s) 478
EclXI CGGCCG 1 cut(s) 498
Eco147I AGGCCT 1 cut(s) 19
Eco24I GRGCYC 2 cut(s) 551, 705
Eco31I GGTCTC 1 cut(s) 260
Eco52I CGGCCG 1 cut(s) 498
Eco81I CCTNAGG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 33
Eco91I GGTNACC 1 cut(s) 553
EcoO65I GGTNACC 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 210
EcoT38I GRGCYC 2 cut(s) 551, 705
Esp3I CGTCTC 1 cut(s) 669
FaiI YATR 2 cut(s) 79, 635
FaqI GGGAC 1 cut(s) 298
FbaI TGATCA 1 cut(s) 426
Fnu4HI GCNGC 6 cut(s) 68, 112, 115, 118, 371, 488
FokI GGATG 1 cut(s) 326
FriOI GRGCYC 2 cut(s) 551, 705
Fsp4HI GCNGC 6 cut(s) 68, 112, 115, 118, 371, 488
GlaI GCGC 1 cut(s) 360
GluI GCNGC 6 cut(s) 68, 112, 115, 118, 371, 488
HaeIII GGCC 3 cut(s) 19, 215, 500
HapII CCGG 8 cut(s) 138, 255, 347, 460, 497, 551, 706, 725
HgaI GACGC 3 cut(s) 479, 550, 579
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 590
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HinfI GANTC 5 cut(s) 169, 182, 218, 302, 517
HpaII CCGG 8 cut(s) 138, 255, 347, 460, 497, 551, 706, 725
HphI GGTGA 5 cut(s) 244, 328, 361, 485, 565
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 7 cut(s) 63, 153, 181, 265, 516, 619, 669
Hpy188III TCNNGA 4 cut(s) 23, 138, 412, 759
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 7 cut(s) 313, 538, 572, 595, 598, 616, 619
HpyAV CCTTC 2 cut(s) 214, 637
HpyCH4III ACNGT 1 cut(s) 321
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 1 cut(s) 658
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 117, 367
HpyF3I CTNAG 2 cut(s) 21, 545
HpySE526I ACGT 1 cut(s) 237
Hsp92I GRCGYC 1 cut(s) 590
HspAI GCGC 1 cut(s) 359
Kpn2I TCCGGA 1 cut(s) 137
Ksp22I TGATCA 1 cut(s) 426
Kzo9I GATC 5 cut(s) 37, 176, 426, 504, 625
LmnI GCTCC 2 cut(s) 399, 462
Lsp1109I GCAGC 4 cut(s) 123, 126, 129, 382
LweI GCATC 1 cut(s) 429
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 3 cut(s) 491, 553, 727
MalI GATC 5 cut(s) 39, 178, 428, 506, 627
MboI GATC 5 cut(s) 37, 176, 426, 504, 625
MboII GAAGA 4 cut(s) 364, 376, 465, 747
MflI RGATCY 2 cut(s) 176, 504
MhlI GDGCHC 3 cut(s) 551, 680, 705
MluCI AATT 1 cut(s) 445
MlyI GAGTC 5 cut(s) 163, 176, 212, 311, 526
MmeI TCCRAC 4 cut(s) 176, 210, 249, 597
MroI TCCGGA 1 cut(s) 137
MspI CCGG 8 cut(s) 138, 255, 347, 460, 497, 551, 706, 725
MspR9I CCNGG 4 cut(s) 212, 497, 551, 706
MvaI CCWGG 1 cut(s) 212
MvnI CGCG 2 cut(s) 359, 490
MwoI GCNNNNNNNGC 3 cut(s) 111, 117, 367
NciI CCSGG 3 cut(s) 497, 551, 706
NdeII GATC 5 cut(s) 37, 176, 426, 504, 625
NlaIV GGNNCC 2 cut(s) 401, 464
NmeAIII GCCGAG 3 cut(s) 526, 664, 762
NmuCI GTSAC 3 cut(s) 491, 553, 727
PceI AGGCCT 1 cut(s) 19
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PkrI GCNGC 6 cut(s) 69, 113, 116, 119, 372, 489
PleI GAGTC 5 cut(s) 163, 176, 212, 310, 525
PpsI GAGTC 5 cut(s) 163, 176, 212, 310, 525
Ppu21I YACGTR 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 210
PspEI GGTNACC 1 cut(s) 553
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 2 cut(s) 401, 464
PsuI RGATCY 2 cut(s) 176, 504
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SatI GCNGC 6 cut(s) 68, 112, 115, 118, 371, 488
Sau3AI GATC 5 cut(s) 37, 176, 426, 504, 625
SchI GAGTC 5 cut(s) 163, 176, 212, 311, 526
ScrFI CCNGG 4 cut(s) 212, 497, 551, 706
SduI GDGCHC 3 cut(s) 551, 680, 705
SetI ASST 5 cut(s) 168, 206, 240, 387, 411
SfaNI GCATC 1 cut(s) 429
SfcI CTRYAG 1 cut(s) 633
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 1 cut(s) 445
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 3 cut(s) 68, 357, 488
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 4 cut(s) 210, 495, 549, 704
TaaI ACNGT 1 cut(s) 321
TaiI ACGT 1 cut(s) 240
TaqI TCGA 1 cut(s) 525
TasI AATT 1 cut(s) 445
TatI WGTACW 1 cut(s) 6
TauI GCSGC 2 cut(s) 70, 490
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 3 cut(s) 491, 553, 727
TseI GCWGC 4 cut(s) 111, 114, 117, 370
Tsp45I GTSAC 3 cut(s) 491, 553, 727
TspDTI ATGAA 1 cut(s) 66
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 1 cut(s) 324
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.