MD11G1249800.v1.1

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010543
Physical Location & Seq
Forward (+)
36203397 .. 36205344
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1249800.v1.1.491

Sequence Viewer

Length: 777 bp
ATGGAATACACAAGCTCAAGGCCTCAAGAATTCAGAGAGAAGCCCTATTCGTATTCCCCCATAAACTCGCCGAGAACCAACAACAATGGCACGGCCACCAACGCCAGCGTCTGCAGCAGCAGCAGCAGCTCGGCGGTGGCGGCTCAAATCCCGACAACTCCGAAAGTCTCAACTCCCAGATCTGACTCCAATCCCTACCCGACAACCTTTGTCCAAGCTGACTCCTCGAACTTCAAACATGTTGTCCAAATGCTCACCGGCTCCTCGGAAACCGTCACTCACCAGTCCTCCCCTAAACCCACCACCGCCACCCACCACCACCACCATCATCAAGATCCAACAATTTTGCCACCCTCCAACAAAAACTTCAACATCCCACCTATCAAAACCGCCCCACCAAAAAAACAGGGCTTCAAGCTCTACGAGCGGAGGAGTACCAATCTCAAAAACACCCTCATGATCAACACCCTCATCCCCAACTTCCAATCGGCTTCTGGGTTTTCGCCACGTCACCAGGAGATCCTGTCCCCGAGCTTGCTCGACTTCCCTTCCCTCACCCTCAGCCCAGTGACGCCGTTGAACGACGACCCCTTCGACAAGTCGGCGGCGTCGCCGTCACTGGGGAACTCTTCGTCGGAGGAGGACAGGGCGATTGCGGAGAAAGGGTTTTACTTGCACCCGTCGCCGAGAGCTACGACGACTCCGAGAGACCAAGAGCCGCGGCTTCTGCCTCTGTTTCCGGTCACGTCACCGAGAGTTTCTGGGTCTTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

259

Amino Acids

28.15

Weight (kDa)

9.35

Isoelectric Point (pI)

61.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 65 - 91 3.3e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 427
AccII CGCG 1 cut(s) 721
AciI CCGC 9 cut(s) 134, 140, 306, 390, 427, 605, 656, 719, 721
AclWI GGATC 2 cut(s) 329, 514
AcoI YGGCCR 1 cut(s) 93
AcsI RAATTY 1 cut(s) 29
AcyI GRCGYC 2 cut(s) 572, 608
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 1 cut(s) 620
AflIII ACRYGT 1 cut(s) 238
AgsI TTSAA 4 cut(s) 235, 370, 415, 580
AjiI CACGTC 2 cut(s) 509, 747
AjnI CCWGG 1 cut(s) 513
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 6 cut(s) 15, 129, 218, 418, 534, 692
AluI AGCT 6 cut(s) 15, 129, 218, 418, 534, 692
Alw26I GTCTC 2 cut(s) 172, 702
AlwI GGATC 2 cut(s) 329, 514
AlwNI CAGNNNCTG 1 cut(s) 111
Ama87I CYCGRG 1 cut(s) 529
AoxI GGCC 2 cut(s) 20, 93
ApeKI GCWGC 5 cut(s) 114, 117, 120, 123, 126
ApoI RAATTY 1 cut(s) 29
ArsI GACNNNNNNTTYG 4 cut(s) 228, 260, 575, 607
AsuHPI GGTGA 5 cut(s) 247, 272, 503, 547, 741
AvaI CYCGRG 1 cut(s) 529
BbsI GAAGAC 1 cut(s) 759
BbvCI CCTCAGC 1 cut(s) 560
BbvI GCAGC 5 cut(s) 126, 129, 132, 135, 138
BccI CCATC 1 cut(s) 333
BceAI ACGGC 3 cut(s) 108, 559, 598
BciT130I CCWGG 1 cut(s) 515
BclI TGATCA 1 cut(s) 459
BcoDI GTCTC 2 cut(s) 172, 702
BfmI CTRYAG 1 cut(s) 112
BglII AGATCT 1 cut(s) 179
BisI GCNGC 9 cut(s) 115, 118, 121, 124, 127, 141, 606, 719, 722
BlsI GCNGC 9 cut(s) 116, 119, 122, 125, 128, 142, 607, 720, 723
Bme1390I CCNGG 1 cut(s) 515
BmeT110I CYCGRG 1 cut(s) 529
BmgBI CACGTC 2 cut(s) 509, 747
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 1 cut(s) 515
BmrI ACTGGG 2 cut(s) 560, 629
BmuI ACTGGG 2 cut(s) 560, 629
BpiI GAAGAC 1 cut(s) 759
Bpu10I CCTNAGC 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 9
BsaHI GRCGYC 2 cut(s) 572, 608
BsaI GGTCTC 1 cut(s) 702
BsaJI CCNNGG 2 cut(s) 264, 719
BsaWI WCCGGW 1 cut(s) 739
BsaXI ACNNNNNCTCC 8 cut(s) 272, 302, 509, 539, 650, 680, 685, 715
Bsc4I CCNNNNNNNGG 1 cut(s) 620
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 3 cut(s) 283, 566, 624
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 2 cut(s) 264, 719
BseGI GGATG 2 cut(s) 372, 471
BseLI CCNNNNNNNGG 1 cut(s) 620
BseMII CTCAG 1 cut(s) 574
BseNI ACTGG 3 cut(s) 283, 566, 624
BseRI GAGGAG 4 cut(s) 214, 253, 445, 653
BseXI GCAGC 5 cut(s) 126, 129, 132, 135, 138
Bsh1236I CGCG 1 cut(s) 721
BshFI GGCC 2 cut(s) 22, 95
BsiHKCI CYCGRG 1 cut(s) 529
BsiSI CCGG 2 cut(s) 258, 740
BslFI GGGAC 1 cut(s) 511
BslI CCNNNNNNNGG 1 cut(s) 620
BsmAI GTCTC 2 cut(s) 172, 702
BsmFI GGGAC 1 cut(s) 511
BsnI GGCC 2 cut(s) 22, 95
Bso31I GGTCTC 1 cut(s) 702
BsoBI CYCGRG 1 cut(s) 529
Bsp143I GATC 4 cut(s) 179, 334, 459, 519
BspACI CCGC 9 cut(s) 134, 140, 306, 390, 427, 605, 656, 719, 721
BspANI GGCC 2 cut(s) 22, 95
BspCNI CTCAG 1 cut(s) 573
BspFNI CGCG 1 cut(s) 721
BspHI TCATGA 1 cut(s) 456
BspLI GGNNCC 1 cut(s) 262
BspMAI CTGCAG 1 cut(s) 116
BspPI GGATC 2 cut(s) 329, 514
BspTNI GGTCTC 1 cut(s) 702
BsrBI CCGCTC 1 cut(s) 427
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 3 cut(s) 283, 566, 624
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 264, 719
BssMI GATC 4 cut(s) 179, 334, 459, 519
BssNI GRCGYC 2 cut(s) 572, 608
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 1 cut(s) 634
BstACI GRCGYC 2 cut(s) 572, 608
BstC8I GCNNGC 2 cut(s) 106, 536
BstDEI CTNAG 1 cut(s) 560
BstDSI CCRYGG 1 cut(s) 719
BstF5I GGATG 2 cut(s) 372, 471
BstFNI CGCG 1 cut(s) 721
BstKTI GATC 4 cut(s) 182, 337, 462, 522
BstMAI GTCTC 2 cut(s) 172, 702
BstMBI GATC 4 cut(s) 179, 334, 459, 519
BstMWI GCNNNNNNNGC 9 cut(s) 101, 114, 120, 123, 126, 140, 424, 682, 727
BstNI CCWGG 1 cut(s) 515
BstNSI RCATGY 1 cut(s) 242
BstSCI CCNGG 1 cut(s) 513
BstSFI CTRYAG 1 cut(s) 112
BstUI CGCG 1 cut(s) 721
BstV1I GCAGC 5 cut(s) 126, 129, 132, 135, 138
BstV2I GAAGAC 1 cut(s) 759
BstX2I RGATCY 3 cut(s) 179, 334, 519
BstYI RGATCY 3 cut(s) 179, 334, 519
BsuRI GGCC 2 cut(s) 22, 95
BtgI CCRYGG 1 cut(s) 719
BtrI CACGTC 2 cut(s) 509, 747
BtsCI GGATG 2 cut(s) 372, 471
BtsIMutI CAGTG 2 cut(s) 573, 617
Cac8I GCNNGC 2 cut(s) 106, 536
CaiI CAGNNNCTG 1 cut(s) 111
CciI TCATGA 1 cut(s) 456
Cfr10I RCCGGY 1 cut(s) 257
Cfr42I CCGCGG 1 cut(s) 722
CseI GACGC 3 cut(s) 97, 580, 597
Csp6I GTAC 1 cut(s) 435
CviAII CATG 2 cut(s) 239, 457
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 1 cut(s) 560
DpnI GATC 4 cut(s) 181, 336, 461, 521
DpnII GATC 4 cut(s) 179, 334, 459, 519
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 1 cut(s) 634
EarI CTCTTC 1 cut(s) 634
Eco147I AGGCCT 1 cut(s) 22
Eco31I GGTCTC 1 cut(s) 702
Eco88I CYCGRG 1 cut(s) 529
EcoRI GAATTC 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 513
FaeI CATG 2 cut(s) 242, 460
FaiI YATR 3 cut(s) 62, 240, 458
FaqI GGGAC 1 cut(s) 511
FatI CATG 2 cut(s) 238, 456
FbaI TGATCA 1 cut(s) 459
Fnu4HI GCNGC 9 cut(s) 115, 118, 121, 124, 127, 141, 606, 719, 722
FokI GGATG 2 cut(s) 359, 458
Fsp4HI GCNGC 9 cut(s) 115, 118, 121, 124, 127, 141, 606, 719, 722
GluI GCNGC 9 cut(s) 115, 118, 121, 124, 127, 141, 606, 719, 722
HaeIII GGCC 2 cut(s) 22, 95
HapII CCGG 2 cut(s) 258, 740
HgaI GACGC 3 cut(s) 97, 580, 597
Hin1I GRCGYC 2 cut(s) 572, 608
Hin1II CATG 2 cut(s) 242, 460
HinfI GANTC 3 cut(s) 185, 221, 700
HpaII CCGG 2 cut(s) 258, 740
HphI GGTGA 5 cut(s) 247, 272, 503, 547, 741
Hpy188I TCNGA 6 cut(s) 35, 162, 184, 268, 637, 705
Hpy188III TCNNGA 5 cut(s) 26, 151, 332, 457, 774
Hpy99I CGWCG 5 cut(s) 587, 613, 637, 685, 700
HpyAV CCTTC 2 cut(s) 558, 601
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 2 cut(s) 508, 746
HpyCH4V TGCA 2 cut(s) 114, 676
HpyF10VI GCNNNNNNNGC 9 cut(s) 101, 114, 120, 123, 126, 140, 424, 682, 727
HpyF3I CTNAG 1 cut(s) 560
HpySE526I ACGT 2 cut(s) 508, 746
Hsp92I GRCGYC 2 cut(s) 572, 608
Hsp92II CATG 2 cut(s) 242, 460
Ksp22I TGATCA 1 cut(s) 459
KspI CCGCGG 1 cut(s) 722
Kzo9I GATC 4 cut(s) 179, 334, 459, 519
LmnI GCTCC 1 cut(s) 266
Lsp1109I GCAGC 5 cut(s) 126, 129, 132, 135, 138
MaeII ACGT 2 cut(s) 508, 746
MaeIII GTNAC 6 cut(s) 274, 509, 568, 615, 742, 747
MalI GATC 4 cut(s) 181, 336, 461, 521
MbiI CCGCTC 1 cut(s) 427
MboI GATC 4 cut(s) 179, 334, 459, 519
MboII GAAGA 3 cut(s) 621, 759, 762
MflI RGATCY 3 cut(s) 179, 334, 519
MluCI AATT 2 cut(s) 29, 342
MlyI GAGTC 3 cut(s) 179, 215, 694
MmeI TCCRAC 3 cut(s) 362, 381, 615
MslI CAYNNNNRTG 1 cut(s) 455
MspA1I CMGCKG 1 cut(s) 721
MspI CCGG 2 cut(s) 258, 740
MspR9I CCNGG 1 cut(s) 515
MvaI CCWGG 1 cut(s) 515
MvnI CGCG 1 cut(s) 721
MwoI GCNNNNNNNGC 9 cut(s) 101, 114, 120, 123, 126, 140, 424, 682, 727
NdeII GATC 4 cut(s) 179, 334, 459, 519
NlaIII CATG 2 cut(s) 242, 460
NlaIV GGNNCC 1 cut(s) 262
NmeAIII GCCGAG 3 cut(s) 96, 110, 711
NmuCI GTSAC 6 cut(s) 274, 509, 568, 615, 742, 747
NspI RCATGY 1 cut(s) 242
PagI TCATGA 1 cut(s) 456
PceI AGGCCT 1 cut(s) 22
PciI ACATGT 1 cut(s) 238
PcsI WCGNNNNNNNCGW 2 cut(s) 591, 701
PkrI GCNGC 9 cut(s) 116, 119, 122, 125, 128, 142, 607, 720, 723
PleI GAGTC 3 cut(s) 179, 215, 694
PpsI GAGTC 3 cut(s) 179, 215, 694
PscI ACATGT 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 513
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 1 cut(s) 262
PstI CTGCAG 1 cut(s) 116
PstNI CAGNNNCTG 1 cut(s) 111
PsuI RGATCY 3 cut(s) 179, 334, 519
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 455
SacII CCGCGG 1 cut(s) 722
SatI GCNGC 9 cut(s) 115, 118, 121, 124, 127, 141, 606, 719, 722
Sau3AI GATC 4 cut(s) 179, 334, 459, 519
SchI GAGTC 3 cut(s) 179, 215, 694
ScrFI CCNGG 1 cut(s) 515
SfcI CTRYAG 1 cut(s) 112
Sfr303I CCGCGG 1 cut(s) 722
SgrBI CCGCGG 1 cut(s) 722
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 2 cut(s) 16, 24
SmoI CTYRAG 2 cut(s) 16, 24
Sse9I AATT 2 cut(s) 29, 342
SseBI AGGCCT 1 cut(s) 22
SsiI CCGC 9 cut(s) 134, 140, 306, 390, 427, 605, 656, 719, 721
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 1 cut(s) 513
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 2 cut(s) 511, 749
TaqI TCGA 3 cut(s) 227, 540, 594
TasI AATT 2 cut(s) 29, 342
TauI GCSGC 4 cut(s) 143, 608, 721, 724
TscAI CASTG 2 cut(s) 573, 624
TseFI GTSAC 6 cut(s) 274, 509, 568, 615, 742, 747
TseI GCWGC 5 cut(s) 114, 117, 120, 123, 126
Tsp45I GTSAC 6 cut(s) 274, 509, 568, 615, 742, 747
TspRI CASTG 2 cut(s) 573, 624
XapI RAATTY 1 cut(s) 29
XceI RCATGY 1 cut(s) 242
XcmI CCANNNNNNNNNTGG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.