pycom03g17680

VQ motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010543
Physical Location & Seq
Forward (+)
18807834 .. 18808649
816 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g17680.1

Sequence Viewer

Length: 816 bp
ATGGAATACATCACAAGCTCAAGACCTCAAGAAATCAGAGAGAAGCCGTATTCGTATTCCCCTATAAACTCGCCGAGGAGCAACAACAATGGCATGACCACCAACGCCAACAGCAGCGTCTGCAGCAGCAGCAGCACGGCGGTGGCGACTCAAATCCCGACAACCCCGAAAGTCTCAACTCCCAGATCTGACTCCAATCCCTACCCGACAACCTTTGTCCAAGCTGACTCCTCGAACTTCAAACATGTTGTCCAAATGCTCACCGGCTCCTCGGAAACTGTCACTCACCAGTCCTCCCCCAAACCCACAGCCGCCGCCCACCACAACAGCCACCACCACCACCACCACCACCACCACCACCACCAAGATCCAACAATTTTGCCACCCTCCAACAAAAACTTCAACATCCCACCAATCAAAACCGCCCCACCGAAAAAGCAGGGCTTCAAGCTCTACGAGCGGCGGAGCACCAATCTCAAAAACACCCTCATCGATCAACACCCTCATCCCCAACTTCAAATCGGCTTCTGGGTTTTCTCCACGTCATCAGGAGATACTCTCCCCGAGCCTGCTCGACTTTCCGTCACTCACCCTCAGCCCGGTGACGCCGTTGAACGACCAGTTCGACAAGTCGGCGTCGTCGCCGTCACTGGGAAATTCTTCGTCGGAGGAGGACAGGGCGATTGCGGAGAAAGGGTTTTACTTGCACCCGTCGCCAAGGGCTACGACGACTCTGAGAGACTCGGAGCCGCGGCTTCTGCCTCTGTTTCCGGTCACTTCGCCGAGAGTTTCTGGGTCGTCTTCTTGATCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VQ

Protein Analysis

272

Amino Acids

29.55

Weight (kDa)

9.23

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VQ PF05678 67 - 93 3.5e-10 VQ motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 460
AccII CGCG 1 cut(s) 752
AciI CCGC 9 cut(s) 140, 312, 315, 423, 460, 463, 687, 750, 752
AclWI GGATC 1 cut(s) 362
AcsI RAATTY 1 cut(s) 656
AcyI GRCGYC 2 cut(s) 606, 636
AfiI CCNNNNNNNGG 2 cut(s) 599, 651
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 5 cut(s) 241, 403, 448, 518, 614
AhdI GACNNNNNGTC 1 cut(s) 581
AjiI CACGTC 1 cut(s) 543
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AleI CACNNNNGTG 1 cut(s) 140
AluBI AGCT 3 cut(s) 18, 224, 451
AluI AGCT 3 cut(s) 18, 224, 451
Alw21I GWGCWC 1 cut(s) 470
Alw26I GTCTC 2 cut(s) 178, 733
AlwI GGATC 1 cut(s) 362
AlwNI CAGNNNCTG 1 cut(s) 120
Ama87I CYCGRG 1 cut(s) 563
ApeKI GCWGC 5 cut(s) 114, 123, 126, 129, 132
ApoI RAATTY 1 cut(s) 656
ArsI GACNNNNNNTTYG 2 cut(s) 234, 266
Asp700I GAANNNNTTC 1 cut(s) 659
AsuC2I CCSGG 1 cut(s) 600
AsuHPI GGTGA 4 cut(s) 253, 278, 581, 614
AvaI CYCGRG 1 cut(s) 563
BbsI GAAGAC 1 cut(s) 793
Bbv12I GWGCWC 1 cut(s) 470
BbvCI CCTCAGC 1 cut(s) 594
BbvI GCAGC 5 cut(s) 126, 135, 138, 141, 144
BceAI ACGGC 4 cut(s) 31, 153, 593, 629
BclI TGATCA 1 cut(s) 807
BcnI CCSGG 1 cut(s) 600
BcoDI GTCTC 2 cut(s) 178, 733
BfmI CTRYAG 1 cut(s) 121
BglII AGATCT 1 cut(s) 185
Bme1390I CCNGG 1 cut(s) 600
BmeRI GACNNNNNGTC 1 cut(s) 581
BmeT110I CYCGRG 1 cut(s) 563
BmgBI CACGTC 1 cut(s) 543
BmiI GGNNCC 2 cut(s) 268, 748
BmrFI CCNGG 1 cut(s) 600
BmrI ACTGGG 1 cut(s) 660
BmuI ACTGGG 1 cut(s) 660
BpiI GAAGAC 1 cut(s) 793
BplI GAGNNNNNCTC 2 cut(s) 543, 575
Bpu10I CCTNAGC 1 cut(s) 594
BpuEI CTTGAG 2 cut(s) 4, 12
BpuMI CCSGG 1 cut(s) 600
Bsa29I ATCGAT 1 cut(s) 492
BsaHI GRCGYC 2 cut(s) 606, 636
BsaJI CCNNGG 4 cut(s) 74, 270, 717, 750
BsaWI WCCGGW 1 cut(s) 770
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 681, 711
Bsc4I CCNNNNNNNGG 2 cut(s) 599, 651
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 3 cut(s) 289, 620, 655
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 4 cut(s) 74, 270, 717, 750
BseGI GGATG 2 cut(s) 405, 505
BseLI CCNNNNNNNGG 2 cut(s) 599, 651
BseMII CTCAG 2 cut(s) 608, 726
BseNI ACTGG 3 cut(s) 289, 620, 655
BseRI GAGGAG 4 cut(s) 91, 220, 259, 684
BseXI GCAGC 5 cut(s) 126, 135, 138, 141, 144
Bsh1236I CGCG 1 cut(s) 752
BshVI ATCGAT 1 cut(s) 492
BsiHKAI GWGCWC 1 cut(s) 470
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 3 cut(s) 264, 600, 771
BslI CCNNNNNNNGG 2 cut(s) 599, 651
BsmAI GTCTC 2 cut(s) 178, 733
BsoBI CYCGRG 1 cut(s) 563
Bsp1286I GDGCHC 1 cut(s) 470
Bsp143I GATC 4 cut(s) 185, 367, 493, 807
BspACI CCGC 9 cut(s) 140, 312, 315, 423, 460, 463, 687, 750, 752
BspCNI CTCAG 2 cut(s) 607, 727
BspDI ATCGAT 1 cut(s) 492
BspFNI CGCG 1 cut(s) 752
BspLI GGNNCC 2 cut(s) 268, 748
BspMAI CTGCAG 1 cut(s) 125
BspPI GGATC 1 cut(s) 362
BsrBI CCGCTC 1 cut(s) 460
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 3 cut(s) 289, 620, 655
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 4 cut(s) 74, 270, 717, 750
BssMI GATC 4 cut(s) 185, 367, 493, 807
BssNI GRCGYC 2 cut(s) 606, 636
BssT1I CCWWGG 1 cut(s) 717
Bst4CI ACNGT 1 cut(s) 280
BstACI GRCGYC 2 cut(s) 606, 636
BstAPI GCANNNNNTGC 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 570
BstDEI CTNAG 2 cut(s) 594, 735
BstDSI CCRYGG 1 cut(s) 750
BstF5I GGATG 2 cut(s) 405, 505
BstFNI CGCG 1 cut(s) 752
BstKTI GATC 4 cut(s) 188, 370, 496, 810
BstMAI GTCTC 2 cut(s) 178, 733
BstMBI GATC 4 cut(s) 185, 367, 493, 807
BstMWI GCNNNNNNNGC 7 cut(s) 120, 123, 129, 132, 457, 713, 758
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 1 cut(s) 598
BstSFI CTRYAG 1 cut(s) 121
BstUI CGCG 1 cut(s) 752
BstV1I GCAGC 5 cut(s) 126, 135, 138, 141, 144
BstV2I GAAGAC 1 cut(s) 793
BstX2I RGATCY 2 cut(s) 185, 367
BstYI RGATCY 2 cut(s) 185, 367
Bsu15I ATCGAT 1 cut(s) 492
BsuTUI ATCGAT 1 cut(s) 492
BtgI CCRYGG 1 cut(s) 750
BtrI CACGTC 1 cut(s) 543
BtsCI GGATG 2 cut(s) 405, 505
BtsIMutI CAGTG 1 cut(s) 648
Cac8I GCNNGC 1 cut(s) 570
CaiI CAGNNNCTG 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 263
Cfr42I CCGCGG 1 cut(s) 753
ClaI ATCGAT 1 cut(s) 492
CseI GACGC 3 cut(s) 106, 614, 625
CviAII CATG 2 cut(s) 94, 245
DdeI CTNAG 2 cut(s) 594, 735
DpnI GATC 4 cut(s) 187, 369, 495, 809
DpnII GATC 4 cut(s) 185, 367, 493, 807
DriI GACNNNNNGTC 1 cut(s) 581
Eam1105I GACNNNNNGTC 1 cut(s) 581
EciI GGCGGA 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 717
Eco88I CYCGRG 1 cut(s) 563
EcoT14I CCWWGG 1 cut(s) 717
ErhI CCWWGG 1 cut(s) 717
FaeI CATG 2 cut(s) 97, 248
FaiI YATR 3 cut(s) 65, 95, 246
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FatI CATG 2 cut(s) 93, 244
FbaI TGATCA 1 cut(s) 807
FokI GGATG 2 cut(s) 392, 492
HapII CCGG 3 cut(s) 264, 600, 771
HgaI GACGC 3 cut(s) 106, 614, 625
Hin1I GRCGYC 2 cut(s) 606, 636
Hin1II CATG 2 cut(s) 97, 248
HinfI GANTC 5 cut(s) 148, 191, 227, 731, 741
HpaII CCGG 3 cut(s) 264, 600, 771
HphI GGTGA 4 cut(s) 253, 278, 581, 614
Hpy188I TCNGA 6 cut(s) 38, 190, 274, 668, 736, 746
Hpy188III TCNNGA 5 cut(s) 21, 29, 157, 549, 805
Hpy99I CGWCG 5 cut(s) 641, 644, 668, 716, 731
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 542
HpyCH4V TGCA 2 cut(s) 123, 707
HpyF10VI GCNNNNNNNGC 7 cut(s) 120, 123, 129, 132, 457, 713, 758
HpyF3I CTNAG 2 cut(s) 594, 735
HpySE526I ACGT 1 cut(s) 542
Hsp92I GRCGYC 2 cut(s) 606, 636
Hsp92II CATG 2 cut(s) 97, 248
Ksp22I TGATCA 1 cut(s) 807
KspI CCGCGG 1 cut(s) 753
Kzo9I GATC 4 cut(s) 185, 367, 493, 807
LmnI GCTCC 4 cut(s) 78, 272, 465, 746
Lsp1109I GCAGC 5 cut(s) 126, 135, 138, 141, 144
MaeII ACGT 1 cut(s) 542
MaeIII GTNAC 5 cut(s) 280, 583, 602, 646, 773
MalI GATC 4 cut(s) 187, 369, 495, 809
MbiI CCGCTC 1 cut(s) 460
MboI GATC 4 cut(s) 185, 367, 493, 807
MboII GAAGA 2 cut(s) 652, 793
MflI RGATCY 2 cut(s) 185, 367
MhlI GDGCHC 1 cut(s) 470
MluCI AATT 2 cut(s) 375, 656
MlyI GAGTC 5 cut(s) 142, 185, 221, 725, 735
MmeI TCCRAC 3 cut(s) 395, 414, 646
MroXI GAANNNNTTC 1 cut(s) 659
MslI CAYNNNNRTG 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 3 cut(s) 264, 600, 771
MspR9I CCNGG 1 cut(s) 600
MvnI CGCG 1 cut(s) 752
MwoI GCNNNNNNNGC 7 cut(s) 120, 123, 129, 132, 457, 713, 758
NciI CCSGG 1 cut(s) 600
NdeII GATC 4 cut(s) 185, 367, 493, 807
NlaIII CATG 2 cut(s) 97, 248
NlaIV GGNNCC 2 cut(s) 268, 748
NmeAIII GCCGAG 2 cut(s) 99, 808
NmuCI GTSAC 5 cut(s) 280, 583, 602, 646, 773
NspI RCATGY 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 140
PciI ACATGT 1 cut(s) 244
PcsI WCGNNNNNNNCGW 2 cut(s) 143, 622
PdmI GAANNNNTTC 1 cut(s) 659
PleI GAGTC 5 cut(s) 142, 185, 221, 725, 735
PpsI GAGTC 5 cut(s) 142, 185, 221, 725, 735
PscI ACATGT 1 cut(s) 244
PspN4I GGNNCC 2 cut(s) 268, 748
PstI CTGCAG 1 cut(s) 125
PstNI CAGNNNCTG 1 cut(s) 120
PsuI RGATCY 2 cut(s) 185, 367
RseI CAYNNNNRTG 1 cut(s) 140
SacII CCGCGG 1 cut(s) 753
Sau3AI GATC 4 cut(s) 185, 367, 493, 807
SchI GAGTC 5 cut(s) 142, 185, 221, 725, 735
ScrFI CCNGG 1 cut(s) 600
SduI GDGCHC 1 cut(s) 470
SetI ASST 6 cut(s) 20, 28, 215, 226, 453, 545
SfcI CTRYAG 1 cut(s) 121
Sfr303I CCGCGG 1 cut(s) 753
SgrBI CCGCGG 1 cut(s) 753
SmiMI CAYNNNNRTG 1 cut(s) 140
SmlI CTYRAG 2 cut(s) 19, 27
SmoI CTYRAG 2 cut(s) 19, 27
Sse9I AATT 2 cut(s) 375, 656
SsiI CCGC 9 cut(s) 140, 312, 315, 423, 460, 463, 687, 750, 752
StyD4I CCNGG 1 cut(s) 598
StyI CCWWGG 1 cut(s) 717
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 1 cut(s) 545
TaqI TCGA 4 cut(s) 233, 492, 574, 625
TasI AATT 2 cut(s) 375, 656
TauI GCSGC 5 cut(s) 314, 317, 463, 752, 755
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 5 cut(s) 280, 583, 602, 646, 773
TseI GCWGC 5 cut(s) 114, 123, 126, 129, 132
Tsp45I GTSAC 5 cut(s) 280, 583, 602, 646, 773
TspGWI ACGGA 1 cut(s) 571
TspRI CASTG 1 cut(s) 655
XapI RAATTY 1 cut(s) 656
XceI RCATGY 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.