FvH4_3g20610

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011080
Physical Location & Seq
Reverse (-)
13642848 .. 13648704
5857 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20610.t1

Sequence Viewer

Length: 2637 bp
ATGAGCAAAGCCGCCGCCGCGGAGGAAGAAGACGAAGACGAGTGCTTCTACGAGTCCCTCGATCGCATTCTCACCTCCTCATCCTGTTCTTGCTCCACCTCCAACTCCGACGACGACACCGACGACATCTCCGATTCCGACGCCGCCAGCGCCAATTATGCCTCCCGCAGGAGGTTTCCGACGCCGAGGTTCCCCATGGAGGCGTACAAGTACGACGTCTGGATCTCCGAGCCGGCCTCCGTCTCCGACCGCCGGTCAAGGCTCCTCCGCGAAATGCGCCTCAGCGGCGACCCGGCGCTGTCTCGGGCCAAGCCCGAGACGGTGGGGCGCTCGGTGTCGTCGGATCATTTGATTAAACGACACTGCGCTCCCGCCGTTTCGCAAATCGTCCGGTCCAAATCCGACGGCGTCGAACAGTGTAACGGCGGTTCTTCTTCTCCTCCTATTCTTTGTTCCGAGACTGAGGCTGCTCCGGCGGCCGAGACCAATTCGTTTGTAAATACACAGAAACAAGATTGCGTTTGTAAATCGGCGAGCGGCAAGAGCAATGGATCTTCTGCCGCGTCTCCTCCGCCGCTGAATAAGCCGCCGTCCGGCAGGAACAGCCGGCGCTGCGACGAGATTCGGATTGAGGTTGAGGAGCCGGAGGAGGAGGAGGAGTTGGAATGTAATGGCGGCAGTGCAAGTGAAGTGTGTACTATTCGGAATCTTGATAATGGGAAGGAGTTTGTGGTGAATGAGATCAGGGAAGACGGGATGTGGAACAAGCTCAAGGAAGTGGGGACCGGGAAGCAGTTGACTATGGAGGAGTTCGAGATGAGTGTGGGACATTCCCCCATTGTTCAAGAACTGATGCGGCGGCAGAATGTGGAGGAGAGTCATAAGGGTGGTGGTGGTGGTTCGGATTCGAATGCCAATGGGGGCGGTGGAGGGGTTCCCAAGATGAAGAAGAGGGGCTGGTTTAAGAGCATTAAGAGTGTTGCCAGTAGCGTGACTGGTCATAAGGAGAGGAGGAGTAGTGATGAGAGGGACACGTCGTCGGAGAAGGGGGGCCGGAGGTCGAGCTCTGCGACTGATGATAGCCAGGATGTGTCGTTTCATGGACCGGAGAGAGTGAGGGTGAGGCAGTATGGGAAGTCTTGTAAGGAGCTTACGGCTGTGTACAAGACGCAGGAGATTCAGGCCCATAATGGGTCTATATGGAGTATTAAGTTTAGCTTGGATGGGAAGTATCTGGCAAGTGCCGGTGAGGATTGTGTGATTCATATATGGCAGGTGATGGAGACCGAGAGGAAGGGTGATTTGTTGATGCAGAAATCAGAAGATAACAATTTGGATTTGTTGTTTATTGCCAATGGATCTTCAGAACCATCTTCAGTCTCGCCCAATAGTTACAGCCATCTAGAAAAGAAGAGGAGAGGGAGGACTTCTATAAGTCGGAAATCATTGAGTTTGGAACATTACGTGATTCCAGAGACAATGTTTGCGCTTTCAGAAAAGCCCATTTCTTCATTCCAAGGTCATGTAGATGATGTGCTTGACCTCTCTTGGTCCAAATCTCAGCACCTGCTGTCATCTTCTATGGATAAAACGGTGCGCCTCTGGCATCTGTCTAGCAAGTCTTGTTTGCGAAAATTTTCACACATTGACTATGTAACTTGCATCCAGTTTAATCCCGTTGATGATAACTACTTCATTAGTGGATCACTTGATGCTAAGGTTCGTATATGGAGCATCCTTGATCACCAAGTGGTTGATTGGAATGATACGCATGAGATGGTCACTGCTGCTTGCTACACTCCTGATGGTCAGGGTGCTCTGGTAGGAACATACAAAGGGAGCTGCCGGTTGTATAACACATCTGATAACAAGTTGCAAGAGAAAAACCAAATAAATCTGCAGAACAAGAAAAAGAAATCTCATCACAAGAAAATTACCGGTTTCCAGTTTGCACCAGGAAGTTCCTCAGAAGTACTAGTTACATCTGCAGATTCACGAATCAGGGTTGTTGATGGTGTTGATCTGGTTCACAAGTTCAAGGGATTTCGCAACACAAATAGCCAAATCTCAGCCACCCTCACAGCAAATGGCAAATATGTGGTATCAGCCAGCGAGGATTCTCATGTATATATATGGAAACATGAAGCTGATTCCCGACCTAGTAGAAGCAAAAGTGTTACTGTCACGCGCTCGTATGAGCATTTCCATAGTCACAACGTATCAGTGGCTGTCTCTTGGCCTGGTGTTGGTGATTCATGGGGACTACAAGATGGAGAACAAAATGGACTTGGACTTGACACCAATCTTGATGAGGTTTCGACAGCCAATCATCCGCCTACTCCAGTTGAGGAGGCCAACGGCAAGGATGGTTCGCGATCAGCATCCGGTTGCACCAATAGCCCCCTTCATGGAACAATTACCAGTGCATCCAACAGTTACTTCTTTGATAGGATTTCAGCAACGTACCCAGAGGAAAAACTTCTTCTAGCTACTAAGAAACGAAGCCCTCGTGTAAGTTTTGATTTTAGCAACGGTGTAAGCCAGAACATTCCAGCCTGGGGTATGGTGATTGTAACCGCTGGCTTGGGTGGTGAAATTAGAACTTTCCAAAATTTTGGGTTGCCGATTCGGATATAA

Protein Analysis

879

Amino Acids

96.56

Weight (kDa)

6.2

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 387 - 428 1.1e-07 THOC3 beta-propeller domain
WD40 PF00400 391 - 424 1.4e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 393 - 433 1.7e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 498 - 579 8.9e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 498 - 625 9.5e-15 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 500 - 716 2.1e-35 WDR5 beta-propeller domain
WD40 PF00400 500 - 535 3.2e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 500 - 562 1.5e-06 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 501 - 595 3e-10 CDC20/Fizzy WD40 domain
EIF3I PF24805 501 - 701 3.2e-09 EIF3I
Beta-prop_THOC3 PF25174 503 - 546 5e-06 THOC3 beta-propeller domain
Beta-prop_IFT122_1st PF23381 504 - 621 1.1e-06 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 505 - 585 5.8e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 508 - 715 6.5e-23 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 510 - 629 5.9e-08 Aladin seven-bladed propeller
Beta-prop_WDR3_2nd PF25172 511 - 712 8.4e-13 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 520 - 626 1.6e-08 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 522 - 712 9.3e-08 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 551 - 720 2.6e-11 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 562 - 723 1.5e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 582 - 714 1.4e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15470 AT5G54200
fragaria_vesca FvH4_3g20610 FvH4_3g20610
malus_domestica MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis RchiOBHm_Chr5g0034631
rosa_laevigata RLG00000033560
rosa_roxburghii Rroxscaffold_1G00045690
rosa_rugosa Rorug05G0147300
rosa_samantha Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana Rw5G021930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1264, 1575
AatII GACGTC 1 cut(s) 219
Acc36I ACCTGC 2 cut(s) 1264, 1575
AccBSI CCGCTC 1 cut(s) 537
AccII CGCG 5 cut(s) 20, 270, 563, 2188, 2374
AclWI GGATC 5 cut(s) 230, 351, 559, 1366, 1711
AcoI YGGCCR 1 cut(s) 477
AcsI RAATTY 2 cut(s) 1634, 2611
AcuI CTGAAG 2 cut(s) 1347, 1359
AcyI GRCGYC 4 cut(s) 141, 182, 216, 408
AdeI CACNNNGTG 1 cut(s) 1750
AfaI GTAC 6 cut(s) 206, 212, 697, 1163, 1974, 2465
AfiI CCNNNNNNNGG 9 cut(s) 168, 171, 199, 252, 593, 1191, 2246, 2408, 2558
AflIII ACRYGT 1 cut(s) 1032
AgeI ACCGGT 1 cut(s) 1937
AgsI TTSAA 2 cut(s) 845, 2038
AhdI GACNNNNNGTC 2 cut(s) 253, 1036
AhlI ACTAGT 1 cut(s) 1975
AjiI CACGTC 1 cut(s) 1035
AjnI CCWGG 4 cut(s) 1083, 1954, 2239, 2555
AjuI GAANNNNNNNTTGG 2 cut(s) 2423, 2455
AluBI AGCT 7 cut(s) 769, 1065, 1150, 1218, 1842, 2147, 2489
AluI AGCT 7 cut(s) 769, 1065, 1150, 1218, 1842, 2147, 2489
Alw21I GWGCWC 2 cut(s) 1067, 1819
AlwI GGATC 5 cut(s) 230, 351, 559, 1366, 1711
AlwNI CAGNNNCTG 2 cut(s) 1567, 2228
Ama87I CYCGRG 2 cut(s) 303, 314
AoxI GGCC 7 cut(s) 234, 306, 477, 1051, 1182, 2237, 2352
ApeKI GCWGC 4 cut(s) 467, 612, 1787, 1842
ApoI RAATTY 2 cut(s) 1634, 2611
AsiGI ACCGGT 1 cut(s) 1937
Asp700I GAANNNNTTC 2 cut(s) 1636, 2478
AspLEI GCGC 9 cut(s) 152, 279, 298, 330, 368, 612, 1489, 1599, 2190
AspS9I GGNCC 7 cut(s) 306, 393, 783, 1051, 1103, 1183, 1551
AsuC2I CCSGG 2 cut(s) 293, 787
AsuII TTCGAA 1 cut(s) 908
AvaI CYCGRG 2 cut(s) 303, 314
AvaII GGWCC 4 cut(s) 393, 783, 1103, 1551
BanII GRGCYC 1 cut(s) 1067
BauI CACGAG 1 cut(s) 2508
BbsI GAAGAC 3 cut(s) 36, 42, 756
Bbv12I GWGCWC 2 cut(s) 1067, 1819
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 4 cut(s) 454, 599, 1774, 1829
BccI CCATC 9 cut(s) 1217, 1273, 1378, 1407, 1771, 1799, 2006, 2264, 2360
BceAI ACGGC 6 cut(s) 359, 421, 439, 574, 1170, 2374
BciT130I CCWGG 4 cut(s) 1085, 1956, 2241, 2557
BclI TGATCA 1 cut(s) 1741
BcnI CCSGG 2 cut(s) 293, 787
BcuI ACTAGT 1 cut(s) 1975
BfaI CTAG 5 cut(s) 1403, 1614, 1976, 2160, 2486
BfmI CTRYAG 2 cut(s) 1898, 1986
BfoI RGCGCY 4 cut(s) 153, 299, 331, 613
BfuAI ACCTGC 2 cut(s) 1264, 1575
BglI GCCNNNNNGGC 1 cut(s) 285
BmcAI AGTACT 1 cut(s) 1974
Bme1390I CCNGG 6 cut(s) 293, 787, 1085, 1956, 2241, 2557
Bme18I GGWCC 4 cut(s) 393, 783, 1103, 1551
BmeRI GACNNNNNGTC 2 cut(s) 253, 1036
BmeT110I CYCGRG 2 cut(s) 303, 314
BmgBI CACGTC 1 cut(s) 1035
BmgT120I GGNCC 7 cut(s) 306, 393, 783, 1051, 1103, 1183, 1551
BmiI GGNNCC 6 cut(s) 191, 263, 642, 784, 936, 1052
BmrFI CCNGG 6 cut(s) 293, 787, 1085, 1956, 2241, 2557
BmsI GCATC 8 cut(s) 843, 1299, 1615, 1671, 1702, 1743, 2390, 2435
BpiI GAAGAC 3 cut(s) 36, 42, 756
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpmI CTGGAG 1 cut(s) 2325
Bpu10I CCTNAGC 2 cut(s) 281, 1716
Bpu14I TTCGAA 1 cut(s) 908
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 2 cut(s) 293, 787
BsaAI YACGTR 1 cut(s) 1465
BsaBI GATNNNNATC 1 cut(s) 2380
BsaHI GRCGYC 4 cut(s) 141, 182, 216, 408
BsaI GGTCTC 2 cut(s) 476, 1277
BsaJI CCNNGG 5 cut(s) 18, 185, 195, 1516, 2556
BsaWI WCCGGW 4 cut(s) 390, 1105, 1937, 2384
BsaXI ACNNNNNCTCC 6 cut(s) 113, 143, 644, 674, 1003, 1033
Bsc4I CCNNNNNNNGG 9 cut(s) 168, 171, 199, 252, 593, 1191, 2246, 2408, 2558
Bse118I RCCGGY 6 cut(s) 232, 252, 606, 1244, 1845, 1937
Bse1I ACTGG 6 cut(s) 984, 1000, 1666, 1945, 2342, 2421
Bse3DI GCAATG 1 cut(s) 553
Bse8I GATNNNNATC 1 cut(s) 2380
BseBI CCWGG 4 cut(s) 1085, 1956, 2241, 2557
BseDI CCNNGG 5 cut(s) 18, 185, 195, 1516, 2556
BseJI GATNNNNATC 1 cut(s) 2380
BseLI CCNNNNNNNGG 9 cut(s) 168, 171, 199, 252, 593, 1191, 2246, 2408, 2558
BseMI GCAATG 1 cut(s) 553
BseMII CTCAG 5 cut(s) 295, 453, 1574, 1980, 2082
BseNI ACTGG 6 cut(s) 984, 1000, 1666, 1945, 2342, 2421
BseX3I CGGCCG 1 cut(s) 477
BseXI GCAGC 4 cut(s) 454, 599, 1774, 1829
Bsh1236I CGCG 5 cut(s) 20, 270, 563, 2188, 2374
Bsh1285I CGRYCG 3 cut(s) 64, 250, 480
BshFI GGCC 7 cut(s) 236, 308, 479, 1053, 1184, 2239, 2354
BshTI ACCGGT 1 cut(s) 1937
BsiEI CGRYCG 3 cut(s) 64, 250, 480
BsiHKAI GWGCWC 2 cut(s) 1067, 1819
BsiHKCI CYCGRG 2 cut(s) 303, 314
BslFI GGGAC 5 cut(s) 40, 796, 840, 1043, 2274
BslI CCNNNNNNNGG 9 cut(s) 168, 171, 199, 252, 593, 1191, 2246, 2408, 2558
BsmBI CGTCTC 3 cut(s) 247, 311, 570
BsmFI GGGAC 5 cut(s) 40, 796, 840, 1043, 2274
BsmI GAATGC 2 cut(s) 66, 916
BsnI GGCC 7 cut(s) 236, 308, 479, 1053, 1184, 2239, 2354
Bso31I GGTCTC 2 cut(s) 476, 1277
BsoBI CYCGRG 2 cut(s) 303, 314
Bsp119I TTCGAA 1 cut(s) 908
Bsp1286I GDGCHC 2 cut(s) 1067, 1819
Bsp1407I TGTACA 1 cut(s) 1161
Bsp19I CCATGG 1 cut(s) 195
Bsp68I TCGCGA 1 cut(s) 2374
BspANI GGCC 7 cut(s) 236, 308, 479, 1053, 1184, 2239, 2354
BspCNI CTCAG 5 cut(s) 294, 454, 1573, 1979, 2081
BspFNI CGCG 5 cut(s) 20, 270, 563, 2188, 2374
BspLI GGNNCC 6 cut(s) 191, 263, 642, 784, 936, 1052
BspMAI CTGCAG 2 cut(s) 1902, 1990
BspMI ACCTGC 2 cut(s) 1264, 1575
BspPI GGATC 5 cut(s) 230, 351, 559, 1366, 1711
BspT104I TTCGAA 1 cut(s) 908
BspTNI GGTCTC 2 cut(s) 476, 1277
BsrBI CCGCTC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 553
BsrFI RCCGGY 6 cut(s) 232, 252, 606, 1244, 1845, 1937
BsrGI TGTACA 1 cut(s) 1161
BsrI ACTGG 6 cut(s) 984, 1000, 1666, 1945, 2342, 2421
BssAI RCCGGY 6 cut(s) 232, 252, 606, 1244, 1845, 1937
BssECI CCNNGG 5 cut(s) 18, 185, 195, 1516, 2556
BssNI GRCGYC 4 cut(s) 141, 182, 216, 408
BssSI CACGAG 1 cut(s) 2508
BssT1I CCWWGG 2 cut(s) 195, 1516
Bst2BI CACGAG 1 cut(s) 2508
Bst2UI CCWGG 4 cut(s) 1085, 1956, 2241, 2557
Bst4CI ACNGT 6 cut(s) 322, 417, 1594, 2182, 2435, 2534
Bst6I CTCTTC 2 cut(s) 944, 1406
BstACI GRCGYC 4 cut(s) 141, 182, 216, 408
BstAUI TGTACA 1 cut(s) 1161
BstBAI YACGTR 1 cut(s) 1465
BstBI TTCGAA 1 cut(s) 908
BstC8I GCNNGC 7 cut(s) 148, 234, 535, 608, 1792, 2110, 2581
BstDEI CTNAG 7 cut(s) 281, 462, 1560, 1716, 1966, 2068, 2493
BstDSI CCRYGG 2 cut(s) 18, 195
BstENI CCTNNNNNAGG 1 cut(s) 166
BstFNI CGCG 5 cut(s) 20, 270, 563, 2188, 2374
BstH2I RGCGCY 4 cut(s) 153, 299, 331, 613
BstHHI GCGC 9 cut(s) 152, 279, 298, 330, 368, 612, 1489, 1599, 2190
BstMCI CGRYCG 3 cut(s) 64, 250, 480
BstNI CCWGG 4 cut(s) 1085, 1956, 2241, 2557
BstSCI CCNGG 6 cut(s) 291, 785, 1083, 1954, 2239, 2555
BstSFI CTRYAG 2 cut(s) 1898, 1986
BstUI CGCG 5 cut(s) 20, 270, 563, 2188, 2374
BstV1I GCAGC 4 cut(s) 454, 599, 1774, 1829
BstV2I GAAGAC 3 cut(s) 36, 42, 756
BstX2I RGATCY 3 cut(s) 222, 551, 1358
BstXI CCANNNNNNTGG 1 cut(s) 2615
BstYI RGATCY 3 cut(s) 222, 551, 1358
BstZI CGGCCG 1 cut(s) 477
BsuRI GGCC 7 cut(s) 236, 308, 479, 1053, 1184, 2239, 2354
BtgI CCRYGG 2 cut(s) 18, 195
BtrI CACGTC 1 cut(s) 1035
BtsI GCAGTG 3 cut(s) 361, 685, 1782
BtsIMutI CAGTG 6 cut(s) 361, 422, 685, 1782, 2229, 2428
BtuMI TCGCGA 1 cut(s) 2374
BveI ACCTGC 2 cut(s) 1264, 1575
Cac8I GCNNGC 7 cut(s) 148, 234, 535, 608, 1792, 2110, 2581
CaiI CAGNNNCTG 2 cut(s) 1567, 2228
CfoI GCGC 9 cut(s) 152, 279, 298, 330, 368, 612, 1489, 1599, 2190
Cfr10I RCCGGY 6 cut(s) 232, 252, 606, 1244, 1845, 1937
Cfr13I GGNCC 7 cut(s) 306, 393, 783, 1051, 1103, 1183, 1551
Cfr42I CCGCGG 1 cut(s) 21
CseI GACGC 5 cut(s) 149, 190, 397, 552, 1177
Csp6I GTAC 6 cut(s) 205, 211, 696, 1162, 1973, 2464
CspAI ACCGGT 1 cut(s) 1937
CviAII CATG 8 cut(s) 196, 1100, 1523, 1772, 2123, 2141, 2256, 2408
CviQI GTAC 6 cut(s) 205, 211, 696, 1162, 1973, 2464
DdeI CTNAG 7 cut(s) 281, 462, 1560, 1716, 1966, 2068, 2493
DraIII CACNNNGTG 1 cut(s) 1750
DriI GACNNNNNGTC 2 cut(s) 253, 1036
EaeI YGGCCR 1 cut(s) 477
EagI CGGCCG 1 cut(s) 477
Eam1104I CTCTTC 2 cut(s) 944, 1406
Eam1105I GACNNNNNGTC 2 cut(s) 253, 1036
EarI CTCTTC 2 cut(s) 944, 1406
EciI GGCGGA 2 cut(s) 561, 2322
Ecl136II GAGCTC 1 cut(s) 1065
EclXI CGGCCG 1 cut(s) 477
Eco130I CCWWGG 2 cut(s) 195, 1516
Eco24I GRGCYC 1 cut(s) 1067
Eco31I GGTCTC 2 cut(s) 476, 1277
Eco47I GGWCC 4 cut(s) 393, 783, 1103, 1551
Eco52I CGGCCG 1 cut(s) 477
Eco53kI GAGCTC 1 cut(s) 1065
Eco57I CTGAAG 2 cut(s) 1347, 1359
Eco88I CYCGRG 2 cut(s) 303, 314
EcoICRI GAGCTC 1 cut(s) 1065
EcoNI CCTNNNNNAGG 1 cut(s) 166
EcoRII CCWGG 4 cut(s) 1083, 1954, 2239, 2555
EcoT14I CCWWGG 2 cut(s) 195, 1516
EcoT38I GRGCYC 1 cut(s) 1067
ErhI CCWWGG 2 cut(s) 195, 1516
Esp3I CGTCTC 3 cut(s) 247, 311, 570
FaeI CATG 8 cut(s) 199, 1103, 1526, 1775, 2126, 2144, 2259, 2411
FalI AAGNNNNNCTT 2 cut(s) 1202, 1234
FaqI GGGAC 5 cut(s) 40, 796, 840, 1043, 2274
FatI CATG 8 cut(s) 195, 1099, 1522, 1771, 2122, 2140, 2255, 2407
FauI CCCGC 2 cut(s) 173, 379
FbaI TGATCA 1 cut(s) 1741
FriOI GRGCYC 1 cut(s) 1067
FspBI CTAG 5 cut(s) 1403, 1614, 1976, 2160, 2486
GlaI GCGC 9 cut(s) 151, 278, 297, 329, 367, 611, 1488, 1598, 2189
GsuI CTGGAG 1 cut(s) 2325
HaeII RGCGCY 4 cut(s) 153, 299, 331, 613
HaeIII GGCC 7 cut(s) 236, 308, 479, 1053, 1184, 2239, 2354
HgaI GACGC 5 cut(s) 149, 190, 397, 552, 1177
HhaI GCGC 9 cut(s) 152, 279, 298, 330, 368, 612, 1489, 1599, 2190
Hin1I GRCGYC 4 cut(s) 141, 182, 216, 408
Hin1II CATG 8 cut(s) 199, 1103, 1526, 1775, 2126, 2144, 2259, 2411
Hin6I GCGC 9 cut(s) 150, 277, 296, 328, 366, 610, 1487, 1597, 2188
HinP1I GCGC 9 cut(s) 150, 277, 296, 328, 366, 610, 1487, 1597, 2188
HincII GTYRAC 1 cut(s) 798
HindII GTYRAC 1 cut(s) 798
Hpy166II GTNNAC 4 cut(s) 696, 798, 1162, 2029
Hpy8I GTNNAC 4 cut(s) 696, 798, 1162, 2029
HpyAV CCTTC 4 cut(s) 715, 1039, 1288, 2414
HpyCH4III ACNGT 6 cut(s) 322, 417, 1594, 2182, 2435, 2534
HpyCH4IV ACGT 5 cut(s) 216, 1034, 1464, 2217, 2462
HpyCH4V TGCA 9 cut(s) 683, 1312, 1662, 1876, 1900, 1952, 1988, 2391, 2426
HpyF3I CTNAG 7 cut(s) 281, 462, 1560, 1716, 1966, 2068, 2493
HpySE526I ACGT 5 cut(s) 216, 1034, 1464, 2217, 2462
Hsp92I GRCGYC 4 cut(s) 141, 182, 216, 408
Hsp92II CATG 8 cut(s) 199, 1103, 1526, 1775, 2126, 2144, 2259, 2411
HspAI GCGC 9 cut(s) 150, 277, 296, 328, 366, 610, 1487, 1597, 2188
KroI GCCGGC 2 cut(s) 232, 606
KroNI GCCGGC 2 cut(s) 234, 608
Ksp22I TGATCA 1 cut(s) 1741
KspI CCGCGG 1 cut(s) 21
LmnI GCTCC 8 cut(s) 98, 267, 373, 475, 640, 1147, 1731, 1839
Lsp1109I GCAGC 4 cut(s) 454, 599, 1774, 1829
LweI GCATC 8 cut(s) 843, 1299, 1615, 1671, 1702, 1743, 2390, 2435
MaeI CTAG 5 cut(s) 1403, 1614, 1976, 2160, 2486
MaeII ACGT 5 cut(s) 216, 1034, 1464, 2217, 2462
MbiI CCGCTC 1 cut(s) 537
MflI RGATCY 3 cut(s) 222, 551, 1358
MhlI GDGCHC 2 cut(s) 1067, 1819
MluCI AATT 8 cut(s) 154, 487, 1330, 1634, 1932, 2415, 2595, 2611
MlyI GAGTC 2 cut(s) 62, 886
MroNI GCCGGC 2 cut(s) 232, 606
MroXI GAANNNNTTC 2 cut(s) 1636, 2478
MseI TTAA 5 cut(s) 354, 963, 972, 1209, 1671
MslI CAYNNNNRTG 2 cut(s) 885, 1527
MspA1I CMGCKG 4 cut(s) 20, 285, 577, 2579
MspR9I CCNGG 6 cut(s) 293, 787, 1085, 1956, 2241, 2557
Mva1269I GAATGC 2 cut(s) 66, 916
MvaI CCWGG 4 cut(s) 1085, 1956, 2241, 2557
MvnI CGCG 5 cut(s) 20, 270, 563, 2188, 2374
NaeI GCCGGC 2 cut(s) 234, 608
NciI CCSGG 2 cut(s) 293, 787
NcoI CCATGG 1 cut(s) 195
NgoMIV GCCGGC 2 cut(s) 232, 606
NlaIII CATG 8 cut(s) 199, 1103, 1526, 1775, 2126, 2144, 2259, 2411
NlaIV GGNNCC 6 cut(s) 191, 263, 642, 784, 936, 1052
NmeAIII GCCGAG 2 cut(s) 210, 505
NmuCI GTSAC 4 cut(s) 991, 1780, 2182, 2210
NruI TCGCGA 1 cut(s) 2374
NspV TTCGAA 1 cut(s) 908
PaqCI CACCTGC 2 cut(s) 1264, 1575
PcsI WCGNNNNNNNCGW 6 cut(s) 57, 117, 120, 129, 1067, 2506
PctI GAATGC 2 cut(s) 66, 916
PdiI GCCGGC 2 cut(s) 234, 608
PdmI GAANNNNTTC 2 cut(s) 1636, 2478
PflFI GACNNNGTC 1 cut(s) 407
PinAI ACCGGT 1 cut(s) 1937
Ple19I CGATCG 1 cut(s) 64
PleI GAGTC 2 cut(s) 61, 885
PpsI GAGTC 2 cut(s) 61, 885
Ppu21I YACGTR 1 cut(s) 1465
Psp124BI GAGCTC 1 cut(s) 1067
Psp6I CCWGG 4 cut(s) 1083, 1954, 2239, 2555
PspGI CCWGG 4 cut(s) 1083, 1954, 2239, 2555
PspN4I GGNNCC 6 cut(s) 191, 263, 642, 784, 936, 1052
PspPI GGNCC 7 cut(s) 306, 393, 783, 1051, 1103, 1183, 1551
PsrI GAACNNNNNNTAC 2 cut(s) 412, 444
PstI CTGCAG 2 cut(s) 1902, 1990
PstNI CAGNNNCTG 2 cut(s) 1567, 2228
PsuI RGATCY 3 cut(s) 222, 551, 1358
PsyI GACNNNGTC 1 cut(s) 407
PvuI CGATCG 1 cut(s) 64
RruI TCGCGA 1 cut(s) 2374
RsaI GTAC 6 cut(s) 206, 212, 697, 1163, 1974, 2465
RsaNI GTAC 6 cut(s) 205, 211, 696, 1162, 1973, 2464
RseI CAYNNNNRTG 2 cut(s) 885, 1527
SacI GAGCTC 1 cut(s) 1067
SacII CCGCGG 1 cut(s) 21
SaqAI TTAA 5 cut(s) 354, 963, 972, 1209, 1671
Sau96I GGNCC 7 cut(s) 306, 393, 783, 1051, 1103, 1183, 1551
ScaI AGTACT 1 cut(s) 1974
SchI GAGTC 2 cut(s) 62, 886
ScrFI CCNGG 6 cut(s) 293, 787, 1085, 1956, 2241, 2557
SduI GDGCHC 2 cut(s) 1067, 1819
SfaNI GCATC 8 cut(s) 843, 1299, 1615, 1671, 1702, 1743, 2390, 2435
SfcI CTRYAG 2 cut(s) 1898, 1986
Sfr303I CCGCGG 1 cut(s) 21
SfuI TTCGAA 1 cut(s) 908
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 4 cut(s) 393, 783, 1103, 1551
SmiMI CAYNNNNRTG 2 cut(s) 885, 1527
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SpeI ACTAGT 1 cut(s) 1975
Sse9I AATT 8 cut(s) 154, 487, 1330, 1634, 1932, 2415, 2595, 2611
SspMI CTAG 5 cut(s) 1403, 1614, 1976, 2160, 2486
SstI GAGCTC 1 cut(s) 1067
StyD4I CCNGG 6 cut(s) 291, 785, 1083, 1954, 2239, 2555
StyI CCWWGG 2 cut(s) 195, 1516
TaaI ACNGT 6 cut(s) 322, 417, 1594, 2182, 2435, 2534
TaiI ACGT 5 cut(s) 219, 1037, 1467, 2220, 2465
TaqI TCGA 6 cut(s) 60, 411, 813, 908, 1061, 2318
TaqII GACCGA 1 cut(s) 1301
TasI AATT 8 cut(s) 154, 487, 1330, 1634, 1932, 2415, 2595, 2611
TatI WGTACW 3 cut(s) 695, 1161, 1972
Tru1I TTAA 5 cut(s) 354, 963, 972, 1209, 1671
Tru9I TTAA 5 cut(s) 354, 963, 972, 1209, 1671
TscAI CASTG 6 cut(s) 368, 422, 685, 1789, 2229, 2428
TseFI GTSAC 4 cut(s) 991, 1780, 2182, 2210
TseI GCWGC 4 cut(s) 467, 612, 1787, 1842
Tsp45I GTSAC 4 cut(s) 991, 1780, 2182, 2210
TspDTI ATGAA 8 cut(s) 959, 1088, 1253, 1500, 1684, 2157, 2244, 2396
TspGWI ACGGA 1 cut(s) 229
TspRI CASTG 6 cut(s) 368, 422, 685, 1789, 2229, 2428
Tth111I GACNNNGTC 1 cut(s) 407
VpaK11BI GGWCC 4 cut(s) 393, 783, 1103, 1551
XagI CCTNNNNNAGG 1 cut(s) 166
XapI RAATTY 2 cut(s) 1634, 2611
XbaI TCTAGA 1 cut(s) 1402
XmnI GAANNNNTTC 2 cut(s) 1636, 2478
XspI CTAG 5 cut(s) 1403, 1614, 1976, 2160, 2486
ZraI GACGTC 1 cut(s) 217
ZrmI AGTACT 1 cut(s) 1974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.