Rw5G021930

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011080
Physical Location & Seq
Reverse (-)
29838571 .. 29843834
5264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021930.1

Sequence Viewer

Length: 2610 bp
ATGAGCAAAGCCGGGGAGGAAGAAGACGAAGACGAGTGCTTCTACGAGTCCCTCGATCGCATTCTTGTCTCCTCCTCCTCCTCCTCCTGTTCTTGCTCCACCTCCACCTCCGACGACGACGACGCCGCAGCCGCCGTCTCTTCTTCTCCTCCCAATTATGCTTCCCGCAAGCGCTTTCCCACCCCAAAGTTCCCAATGGGGGCCTACAAGTACGACGTCTGGATCTCCGAGCCCGCCTCCGTCTCCGACCGCCGCTTACGTCTCCTCCGCGAAATGGGCCTCAGCGGCGACCCGGCCCTCTCGCGCGCCAAGCCCGACCCGGTGGGCCGCTCGGCCTCGTCGGATCATTTGACTAAACGACACAGCCCTGCCGCCGTTCCGGAAATCGTCCGGTCCAAATCGGACGGCGTCGAACGCTGCAACGGCGCTGCATCCGCTAGTTCGTCGCCTATTCTTTGTTCCGAGATTGAGGCTCCGGCTCCGGCCCCGGCCGAGACCGAGACCAAAAATTCGTTTGTAAATACACAGAAAAAAAAAGATTGCGTCGTTTGTAAATCGGCGAGCGGCAAGAGCAATGGACCTTCGCCGTCGCCGCCGAATAGGCCGCCGTCCGGGAAGAACGGCCGGCGGTGCGGCGACGAGATTGGGTTAGAGGCGGAGGAGGAGGAGGAGGAGAGTGAAGTCTGTACTATTCGGAATCTTGATAATGGGAAGGAGTTTGTGGTGAATGAGATCAGGGAAGATGGGATGTGGAACAAGCTTAAGGAAGTGGGGACCGGGAAGCAGTTGACCATGGAGGAGTTTGAGATGAGTGTGGGCCATTCCCCCATTGTTCAGGAGCTGATGCGGCGGCAGAATGTGGAGGAGAGTCACAAGGCCGGTGGTGGTTCGGATTCGAATGCCAATGGGGGCGGTGGGGGGATTCCCAAGTTGAAGAAGAGGGGTTGGTTCAAGAGCATAAAGAGTGTTGCCAGTACTGTGACTGGGCATAAGGAGAGAAGGAGCAGTGATGAGAGGGACACTTCGTCGGAGAAGGGGGGCCGGAGGTCAAGCTCTGCCACTGATGATAGCCAGGATGTGTCGTTTCACGGGCCGGAGAGAGTGAGGGTCAGGCAGTATGGCAAGTCTTGTAAGGAGCTGACGGCTATGTACAAGACACAGGAGATTCAGGCGCATAATGGGTCTATTTGGAGTATTAAGTTTAGCTTGGATGGGAAGTATCTGGCGAGTGCCGGTGAGGATTGTGTGATTCATATATGGCAGGTGATGGAGACGGAGAGGAAGGGTGACCTGTTGATGCAGAAATCGGAAGATAACAATTTGGATTTGTTGTTTATTGCCAATGGATCTTCAGAACCATGTTCCGTCTCGCCCAATAGTTACAGCCATCTAGAAAAAAAGAAGAGAGGGAGGACGTCTATCAGTCGGAAATCATTGAGTTTGGAACATTATGTGATTCCAGAGACTATGTTTGCACTCTCAGAAAAGCCCATCTGTTCATTCCAAGGTCATGTAGATGATGTGCTTGACCTCTCTTGGTCCAAGTCTCAGCGCCTGCTCTCATCTTCTATGGATAAAACGGTTCACCTCTGGGATTTGCCTAGCAAGTCTTGTTTGCAGAAGTTTTCACACATTGACTATGTAACTTGCATCCAGTTTAATCCCGTTGATGATAACTACTTCATTAGTGGATCACTTGATGCTAAGGTTCGTATATGGAGTATCACTGATCGCCAAGTGGTTGATTGGAATGATATGCATGAGATGGTCACTGCTGCTTGCTACACTCCTGATGGCCAGGGTGCTCTAGTTGGAACCTACAAAGGGAGCTGCCGGTTATACAACACATCTGATAATAAGTTGCAAGAGAAAAGCCAAATAAATCTGCAGAACAAGAAAAAGAAATCTCATCACAAGAAAATTACTGGTTTCCAGTTTGCACCAGGAAGTTCCTCGGAAGTACTAGTTACATCTGCAGATTCACGAGTCAGGGTTGTCGATGGTATTGATCTAGTTCACAAGTTCAAGGGATTTCGTAACACAAATAGCCAAATCTCAGCAACCCTCACAGCAAACGGTAAATATGTGGTATCTGCCAGCGAGGATTCTCATGTATATATATGGAAACATGAAGCTGATTCGCGCCCTAGCAGAAGCAAAAGTGTTACTGTTACACGGTCGTATGAGCATTTTCATAGTCACAATGTATCAGTGGCCGTCTCTTGGCCTGGTGTTGGTGATTCATGGGGACTACAAGATGGAGATCAAAACAATCTTGATGAGGTTTCTACAGCCAATCATCCCCCTACTCCTGTTGAGGAGGAGGCTGATGGCAAGGATGGTTCACGATCAGCATCGGGTTGCACCAATAGCCCTCTCCATGGAACAATTACTAGTGCATCCAACAGTTACTTCTTTGATAGAATTTCGGCAACGTGGCCTGAGGAAAAACTTCTTCTAGCTACTAAGAAACGAAGCCCTCGTGTAAGTTTTGATTTTAGCAATGGTGTAAGCCAGAACATTCCGGCCTGGGGTATGGTGATTGTAACCGCTGGCTTGGGAGGGGAAATTAGAACTTTCCAAAATTTTGGGTTGCCGATTCGGATTTAA

Protein Analysis

869

Amino Acids

95.41

Weight (kDa)

6.42

Isoelectric Point (pI)

56.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 381 - 424 6.1e-08 THOC3 beta-propeller domain
WD40 PF00400 386 - 420 1.2e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 389 - 429 1.6e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 494 - 575 1.3e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 495 - 621 3.8e-14 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 496 - 712 1.7e-34 WDR5 beta-propeller domain
WD40 PF00400 496 - 532 2.2e-07 WD domain, G-beta repeat
EIF3I PF24805 496 - 697 1.7e-09 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 496 - 558 7e-08 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 497 - 591 1.1e-10 CDC20/Fizzy WD40 domain
WDR55 PF24796 498 - 626 8.9e-07 WDR55
Beta-prop_THOC3 PF25174 500 - 543 2.8e-06 THOC3 beta-propeller domain
Beta-prop_IFT122_1st PF23381 501 - 617 3.1e-07 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 501 - 580 1.4e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 503 - 626 3.4e-09 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 504 - 711 1.1e-22 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 507 - 708 9.9e-12 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 516 - 622 3.4e-08 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 547 - 716 1.3e-11 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 558 - 720 1.7e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 566 - 716 9.5e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 578 - 710 4.6e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15470 AT5G54200
fragaria_vesca FvH4_3g20610 FvH4_3g20610
malus_domestica MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis RchiOBHm_Chr5g0034631
rosa_laevigata RLG00000033560
rosa_roxburghii Rroxscaffold_1G00045690
rosa_rugosa Rorug05G0147300
rosa_samantha Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana Rw5G021930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1252
AatII GACGTC 2 cut(s) 219, 1418
Acc36I ACCTGC 1 cut(s) 1252
AccBSI CCGCTC 2 cut(s) 330, 564
AccII CGCG 4 cut(s) 270, 304, 306, 2143
AccIII TCCGGA 1 cut(s) 379
AclWI GGATC 4 cut(s) 230, 351, 1354, 1699
AcoI YGGCCR 4 cut(s) 489, 622, 1795, 2214
AcsI RAATTY 3 cut(s) 508, 2424, 2584
AcuI CTGAAG 1 cut(s) 1335
AcyI GRCGYC 4 cut(s) 123, 216, 408, 1415
AfaI GTAC 5 cut(s) 212, 688, 976, 1151, 1962
AfeI AGCGCT 1 cut(s) 173
AfiI CCNNNNNNNGG 8 cut(s) 199, 274, 611, 1824, 2223, 2234, 2381, 2531
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 3 cut(s) 934, 952, 2026
AhdI GACNNNNNGTC 1 cut(s) 1024
AhlI ACTAGT 2 cut(s) 1963, 2393
AjnI CCWGG 5 cut(s) 1071, 1797, 1942, 2227, 2528
AjuI GAANNNNNNNTTGG 2 cut(s) 2396, 2428
AluBI AGCT 8 cut(s) 760, 841, 1053, 1138, 1206, 1830, 2135, 2462
AluI AGCT 8 cut(s) 760, 841, 1053, 1138, 1206, 1830, 2135, 2462
Alw21I GWGCWC 1 cut(s) 1807
AlwI GGATC 4 cut(s) 230, 351, 1354, 1699
AlwNI CAGNNNCTG 2 cut(s) 841, 1555
Aor13HI TCCGGA 1 cut(s) 379
Aor51HI AGCGCT 1 cut(s) 173
ApeKI GCWGC 5 cut(s) 128, 417, 428, 1775, 1830
ApoI RAATTY 3 cut(s) 508, 2424, 2584
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
Asp700I GAANNNNTTC 1 cut(s) 2451
AspLEI GCGC 7 cut(s) 174, 306, 308, 428, 1174, 1554, 2145
AsuC2I CCSGG 6 cut(s) 13, 293, 320, 488, 613, 778
AsuHPI GGTGA 7 cut(s) 736, 1247, 1276, 1298, 1577, 2249, 2551
AsuII TTCGAA 1 cut(s) 896
AvaII GGWCC 4 cut(s) 393, 578, 774, 1539
AxyI CCTNAGG 1 cut(s) 2442
BalI TGGCCA 1 cut(s) 1797
BanII GRGCYC 1 cut(s) 234
BauI CACGAG 2 cut(s) 1983, 2481
BbsI GAAGAC 2 cut(s) 30, 36
Bbv12I GWGCWC 1 cut(s) 1807
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 5 cut(s) 140, 404, 415, 1762, 1817
BceAI ACGGC 9 cut(s) 119, 359, 421, 439, 571, 592, 637, 1158, 2201
BciT130I CCWGG 5 cut(s) 1073, 1799, 1944, 2229, 2530
BcnI CCSGG 6 cut(s) 13, 293, 320, 488, 613, 778
BcuI ACTAGT 2 cut(s) 1963, 2393
BfaI CTAG 9 cut(s) 438, 1391, 1602, 1808, 1964, 2012, 2148, 2394, 2459
BfmI CTRYAG 3 cut(s) 1886, 1974, 2289
BfoI RGCGCY 3 cut(s) 175, 429, 1555
BfrI CTTAAG 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 1252
BglI GCCNNNNNGGC 2 cut(s) 285, 601
BmcAI AGTACT 2 cut(s) 976, 1962
Bme18I GGWCC 4 cut(s) 393, 578, 774, 1539
BmeRI GACNNNNNGTC 1 cut(s) 1024
BmiI GGNNCC 7 cut(s) 202, 474, 480, 486, 775, 1040, 1816
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 7 cut(s) 440, 834, 1287, 1659, 1690, 2363, 2408
BmuI ACTGGG 1 cut(s) 993
BpiI GAAGAC 2 cut(s) 30, 36
BplI GAGNNNNNCTC 2 cut(s) 221, 253
Bpu10I CCTNAGC 2 cut(s) 281, 1704
Bpu14I TTCGAA 1 cut(s) 896
BpuMI CCSGG 6 cut(s) 13, 293, 320, 488, 613, 778
BsaBI GATNNNNATC 2 cut(s) 2262, 2353
BsaHI GRCGYC 4 cut(s) 123, 216, 408, 1415
BsaI GGTCTC 2 cut(s) 488, 494
BsaJI CCNNGG 8 cut(s) 12, 486, 792, 1504, 1798, 1953, 2380, 2529
BsaWI WCCGGW 2 cut(s) 379, 390
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 8 cut(s) 199, 274, 611, 1824, 2223, 2234, 2381, 2531
Bse118I RCCGGY 4 cut(s) 624, 878, 1232, 1833
Bse1I ACTGG 5 cut(s) 972, 988, 1654, 1930, 1933
Bse21I CCTNAGG 1 cut(s) 2442
Bse3DI GCAATG 2 cut(s) 580, 2509
Bse8I GATNNNNATC 2 cut(s) 2262, 2353
BseAI TCCGGA 1 cut(s) 379
BseBI CCWGG 5 cut(s) 1073, 1799, 1944, 2229, 2530
BseDI CCNNGG 8 cut(s) 12, 486, 792, 1504, 1798, 1953, 2380, 2529
BseGI GGATG 8 cut(s) 431, 753, 1081, 1216, 1650, 2299, 2344, 2399
BseJI GATNNNNATC 2 cut(s) 2262, 2353
BseLI CCNNNNNNNGG 8 cut(s) 199, 274, 611, 1824, 2223, 2234, 2381, 2531
BseMI GCAATG 2 cut(s) 580, 2509
BseMII CTCAG 5 cut(s) 295, 1494, 1562, 2070, 2433
BseNI ACTGG 5 cut(s) 972, 988, 1654, 1930, 1933
BsePI GCGCGC 1 cut(s) 304
BseX3I CGGCCG 2 cut(s) 489, 622
BseXI GCAGC 5 cut(s) 140, 404, 415, 1762, 1817
Bsh1236I CGCG 4 cut(s) 270, 304, 306, 2143
Bsh1285I CGRYCG 5 cut(s) 58, 250, 492, 625, 2180
BsiEI CGRYCG 5 cut(s) 58, 250, 492, 625, 2180
BsiHKAI GWGCWC 1 cut(s) 1807
BslFI GGGAC 4 cut(s) 34, 787, 1031, 2262
BslI CCNNNNNNNGG 8 cut(s) 199, 274, 611, 1824, 2223, 2234, 2381, 2531
BsmBI CGTCTC 6 cut(s) 142, 247, 266, 1265, 1372, 2224
BsmFI GGGAC 4 cut(s) 34, 787, 1031, 2262
BsmI GAATGC 2 cut(s) 60, 904
Bso31I GGTCTC 2 cut(s) 488, 494
Bsp119I TTCGAA 1 cut(s) 896
Bsp1286I GDGCHC 2 cut(s) 234, 1807
Bsp13I TCCGGA 1 cut(s) 379
Bsp1407I TGTACA 1 cut(s) 1149
Bsp19I CCATGG 2 cut(s) 792, 2380
BspCNI CTCAG 5 cut(s) 294, 1493, 1561, 2069, 2434
BspEI TCCGGA 1 cut(s) 379
BspFNI CGCG 4 cut(s) 270, 304, 306, 2143
BspLI GGNNCC 7 cut(s) 202, 474, 480, 486, 775, 1040, 1816
BspMAI CTGCAG 2 cut(s) 1890, 1978
BspMI ACCTGC 1 cut(s) 1252
BspPI GGATC 4 cut(s) 230, 351, 1354, 1699
BspT104I TTCGAA 1 cut(s) 896
BspTI CTTAAG 1 cut(s) 761
BspTNI GGTCTC 2 cut(s) 488, 494
BsrBI CCGCTC 2 cut(s) 330, 564
BsrDI GCAATG 2 cut(s) 580, 2509
BsrFI RCCGGY 4 cut(s) 624, 878, 1232, 1833
BsrGI TGTACA 1 cut(s) 1149
BsrI ACTGG 5 cut(s) 972, 988, 1654, 1930, 1933
BssAI RCCGGY 4 cut(s) 624, 878, 1232, 1833
BssECI CCNNGG 8 cut(s) 12, 486, 792, 1504, 1798, 1953, 2380, 2529
BssHII GCGCGC 1 cut(s) 304
BssNI GRCGYC 4 cut(s) 123, 216, 408, 1415
BssSI CACGAG 2 cut(s) 1983, 2481
BssT1I CCWWGG 3 cut(s) 792, 1504, 2380
Bst2BI CACGAG 2 cut(s) 1983, 2481
Bst2UI CCWGG 5 cut(s) 1073, 1799, 1944, 2229, 2530
Bst4CI ACNGT 6 cut(s) 979, 1582, 2078, 2170, 2178, 2408
Bst6I CTCTTC 3 cut(s) 145, 932, 1397
BstACI GRCGYC 4 cut(s) 123, 216, 408, 1415
BstAFI CTTAAG 1 cut(s) 761
BstAUI TGTACA 1 cut(s) 1149
BstBI TTCGAA 1 cut(s) 896
BstC8I GCNNGC 9 cut(s) 170, 234, 306, 562, 626, 1556, 1780, 2098, 2554
BstDEI CTNAG 7 cut(s) 281, 1480, 1548, 1704, 2056, 2442, 2466
BstDSI CCRYGG 2 cut(s) 792, 2380
BstEII GGTNACC 1 cut(s) 1286
BstF5I GGATG 8 cut(s) 431, 753, 1081, 1216, 1650, 2299, 2344, 2399
BstFNI CGCG 4 cut(s) 270, 304, 306, 2143
BstH2I RGCGCY 3 cut(s) 175, 429, 1555
BstHHI GCGC 7 cut(s) 174, 306, 308, 428, 1174, 1554, 2145
BstMCI CGRYCG 5 cut(s) 58, 250, 492, 625, 2180
BstNI CCWGG 5 cut(s) 1073, 1799, 1944, 2229, 2530
BstPI GGTNACC 1 cut(s) 1286
BstSFI CTRYAG 3 cut(s) 1886, 1974, 2289
BstUI CGCG 4 cut(s) 270, 304, 306, 2143
BstV1I GCAGC 5 cut(s) 140, 404, 415, 1762, 1817
BstV2I GAAGAC 2 cut(s) 30, 36
BstX2I RGATCY 2 cut(s) 222, 1346
BstXI CCANNNNNNTGG 1 cut(s) 2588
BstYI RGATCY 2 cut(s) 222, 1346
BstZI CGGCCG 2 cut(s) 489, 622
Bsu36I CCTNAGG 1 cut(s) 2442
BtgI CCRYGG 2 cut(s) 792, 2380
BtsCI GGATG 8 cut(s) 431, 753, 1081, 1216, 1650, 2299, 2344, 2399
BtsI GCAGTG 2 cut(s) 1012, 1770
BtsIMutI CAGTG 5 cut(s) 1012, 1059, 1725, 1770, 2217
BveI ACCTGC 1 cut(s) 1252
Cac8I GCNNGC 9 cut(s) 170, 234, 306, 562, 626, 1556, 1780, 2098, 2554
CaiI CAGNNNCTG 2 cut(s) 841, 1555
CfoI GCGC 7 cut(s) 174, 306, 308, 428, 1174, 1554, 2145
Cfr10I RCCGGY 4 cut(s) 624, 878, 1232, 1833
CseI GACGC 3 cut(s) 131, 397, 532
Csp6I GTAC 5 cut(s) 211, 687, 975, 1150, 1961
CspCI CAANNNNNGTGG 2 cut(s) 862, 897
CviAII CATG 8 cut(s) 793, 1359, 1511, 1760, 2111, 2129, 2244, 2381
CviQI GTAC 5 cut(s) 211, 687, 975, 1150, 1961
DdeI CTNAG 7 cut(s) 281, 1480, 1548, 1704, 2056, 2442, 2466
DriI GACNNNNNGTC 1 cut(s) 1024
EaeI YGGCCR 4 cut(s) 489, 622, 1795, 2214
EagI CGGCCG 2 cut(s) 489, 622
Eam1104I CTCTTC 3 cut(s) 145, 932, 1397
Eam1105I GACNNNNNGTC 1 cut(s) 1024
EarI CTCTTC 3 cut(s) 145, 932, 1397
EciI GGCGGA 1 cut(s) 671
EclXI CGGCCG 2 cut(s) 489, 622
Eco130I CCWWGG 3 cut(s) 792, 1504, 2380
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 2 cut(s) 488, 494
Eco47I GGWCC 4 cut(s) 393, 578, 774, 1539
Eco47III AGCGCT 1 cut(s) 173
Eco52I CGGCCG 2 cut(s) 489, 622
Eco57I CTGAAG 1 cut(s) 1335
Eco81I CCTNAGG 1 cut(s) 2442
Eco91I GGTNACC 1 cut(s) 1286
EcoO109I RGGNCCY 1 cut(s) 201
EcoO65I GGTNACC 1 cut(s) 1286
EcoRII CCWGG 5 cut(s) 1071, 1797, 1942, 2227, 2528
EcoT14I CCWWGG 3 cut(s) 792, 1504, 2380
EcoT22I ATGCAT 1 cut(s) 1761
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 3 cut(s) 792, 1504, 2380
Esp3I CGTCTC 6 cut(s) 142, 247, 266, 1265, 1372, 2224
FaeI CATG 8 cut(s) 796, 1362, 1514, 1763, 2114, 2132, 2247, 2384
FalI AAGNNNNNCTT 2 cut(s) 1190, 1222
FaqI GGGAC 4 cut(s) 34, 787, 1031, 2262
FatI CATG 8 cut(s) 792, 1358, 1510, 1759, 2110, 2128, 2243, 2380
FauI CCCGC 2 cut(s) 173, 241
FokI GGATG 8 cut(s) 418, 760, 1088, 1223, 1637, 2286, 2351, 2386
FriOI GRGCYC 1 cut(s) 234
FspBI CTAG 9 cut(s) 438, 1391, 1602, 1808, 1964, 2012, 2148, 2394, 2459
GlaI GCGC 7 cut(s) 173, 305, 307, 427, 1173, 1553, 2144
HaeII RGCGCY 3 cut(s) 175, 429, 1555
HgaI GACGC 3 cut(s) 131, 397, 532
HhaI GCGC 7 cut(s) 174, 306, 308, 428, 1174, 1554, 2145
Hin1I GRCGYC 4 cut(s) 123, 216, 408, 1415
Hin1II CATG 8 cut(s) 796, 1362, 1514, 1763, 2114, 2132, 2247, 2384
Hin6I GCGC 7 cut(s) 172, 304, 306, 426, 1172, 1552, 2143
HinP1I GCGC 7 cut(s) 172, 304, 306, 426, 1172, 1552, 2143
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HindIII AAGCTT 1 cut(s) 758
HphI GGTGA 7 cut(s) 736, 1247, 1276, 1298, 1577, 2249, 2551
Hpy166II GTNNAC 4 cut(s) 789, 1585, 2017, 2345
Hpy8I GTNNAC 4 cut(s) 789, 1585, 2017, 2345
HpyAV CCTTC 5 cut(s) 591, 706, 993, 1027, 1276
HpyCH4III ACNGT 6 cut(s) 979, 1582, 2078, 2170, 2178, 2408
HpyCH4IV ACGT 4 cut(s) 216, 259, 1415, 2435
HpyF3I CTNAG 7 cut(s) 281, 1480, 1548, 1704, 2056, 2442, 2466
HpySE526I ACGT 4 cut(s) 216, 259, 1415, 2435
Hsp92I GRCGYC 4 cut(s) 123, 216, 408, 1415
Hsp92II CATG 8 cut(s) 796, 1362, 1514, 1763, 2114, 2132, 2247, 2384
HspAI GCGC 7 cut(s) 172, 304, 306, 426, 1172, 1552, 2143
Kpn2I TCCGGA 1 cut(s) 379
KroI GCCGGC 1 cut(s) 624
KroNI GCCGGC 1 cut(s) 626
LmnI GCTCC 7 cut(s) 101, 478, 484, 838, 1002, 1135, 1827
Lsp1109I GCAGC 5 cut(s) 140, 404, 415, 1762, 1817
LweI GCATC 7 cut(s) 440, 834, 1287, 1659, 1690, 2363, 2408
MaeI CTAG 9 cut(s) 438, 1391, 1602, 1808, 1964, 2012, 2148, 2394, 2459
MaeII ACGT 4 cut(s) 216, 259, 1415, 2435
MbiI CCGCTC 2 cut(s) 330, 564
MflI RGATCY 2 cut(s) 222, 1346
MhlI GDGCHC 2 cut(s) 234, 1807
MlsI TGGCCA 1 cut(s) 1797
MluCI AATT 8 cut(s) 154, 508, 1318, 1920, 2388, 2424, 2568, 2584
MluNI TGGCCA 1 cut(s) 1797
MlyI GAGTC 3 cut(s) 56, 877, 1995
MmeI TCCRAC 7 cut(s) 135, 270, 321, 1008, 1406, 1792, 2427
Mox20I TGGCCA 1 cut(s) 1797
Mph1103I ATGCAT 1 cut(s) 1761
MroI TCCGGA 1 cut(s) 379
MroNI GCCGGC 1 cut(s) 624
MroXI GAANNNNTTC 1 cut(s) 2451
MscI TGGCCA 1 cut(s) 1797
MseI TTAA 4 cut(s) 762, 1197, 1659, 2608
MslI CAYNNNNRTG 1 cut(s) 1515
Msp20I TGGCCA 1 cut(s) 1797
MspA1I CMGCKG 2 cut(s) 285, 2552
MspCI CTTAAG 1 cut(s) 761
Mva1269I GAATGC 2 cut(s) 60, 904
MvaI CCWGG 5 cut(s) 1073, 1799, 1944, 2229, 2530
MvnI CGCG 4 cut(s) 270, 304, 306, 2143
NaeI GCCGGC 1 cut(s) 626
NciI CCSGG 6 cut(s) 13, 293, 320, 488, 613, 778
NcoI CCATGG 2 cut(s) 792, 2380
NgoMIV GCCGGC 1 cut(s) 624
NlaIII CATG 8 cut(s) 796, 1362, 1514, 1763, 2114, 2132, 2247, 2384
NlaIV GGNNCC 7 cut(s) 202, 474, 480, 486, 775, 1040, 1816
NmeAIII GCCGAG 2 cut(s) 311, 517
NmuCI GTSAC 5 cut(s) 869, 979, 1286, 1768, 2198
NsiI ATGCAT 1 cut(s) 1761
NspV TTCGAA 1 cut(s) 896
PaqCI CACCTGC 1 cut(s) 1252
PauI GCGCGC 1 cut(s) 304
PcsI WCGNNNNNNNCGW 2 cut(s) 51, 2479
PctI GAATGC 2 cut(s) 60, 904
PdiI GCCGGC 1 cut(s) 626
PdmI GAANNNNTTC 1 cut(s) 2451
PflFI GACNNNGTC 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 611
Ple19I CGATCG 1 cut(s) 58
PleI GAGTC 3 cut(s) 55, 876, 1994
PpsI GAGTC 3 cut(s) 55, 876, 1994
Psp6I CCWGG 5 cut(s) 1071, 1797, 1942, 2227, 2528
PspEI GGTNACC 1 cut(s) 1286
PspGI CCWGG 5 cut(s) 1071, 1797, 1942, 2227, 2528
PspN4I GGNNCC 7 cut(s) 202, 474, 480, 486, 775, 1040, 1816
PstI CTGCAG 2 cut(s) 1890, 1978
PstNI CAGNNNCTG 2 cut(s) 841, 1555
PsuI RGATCY 2 cut(s) 222, 1346
PsyI GACNNNGTC 1 cut(s) 407
PteI GCGCGC 1 cut(s) 304
PvuI CGATCG 1 cut(s) 58
RsaI GTAC 5 cut(s) 212, 688, 976, 1151, 1962
RsaNI GTAC 5 cut(s) 211, 687, 975, 1150, 1961
RseI CAYNNNNRTG 1 cut(s) 1515
SaqAI TTAA 4 cut(s) 762, 1197, 1659, 2608
ScaI AGTACT 2 cut(s) 976, 1962
SchI GAGTC 3 cut(s) 56, 877, 1995
SduI GDGCHC 2 cut(s) 234, 1807
SfaNI GCATC 7 cut(s) 440, 834, 1287, 1659, 1690, 2363, 2408
SfcI CTRYAG 3 cut(s) 1886, 1974, 2289
SfuI TTCGAA 1 cut(s) 896
SinI GGWCC 4 cut(s) 393, 578, 774, 1539
SmiMI CAYNNNNRTG 1 cut(s) 1515
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
SpeI ACTAGT 2 cut(s) 1963, 2393
Sse9I AATT 8 cut(s) 154, 508, 1318, 1920, 2388, 2424, 2568, 2584
SspMI CTAG 9 cut(s) 438, 1391, 1602, 1808, 1964, 2012, 2148, 2394, 2459
StyI CCWWGG 3 cut(s) 792, 1504, 2380
TaaI ACNGT 6 cut(s) 979, 1582, 2078, 2170, 2178, 2408
TaiI ACGT 4 cut(s) 219, 262, 1418, 2438
TaqI TCGA 4 cut(s) 54, 411, 896, 1998
TaqII GACCGA 1 cut(s) 512
TasI AATT 8 cut(s) 154, 508, 1318, 1920, 2388, 2424, 2568, 2584
TatI WGTACW 4 cut(s) 686, 974, 1149, 1960
Tru1I TTAA 4 cut(s) 762, 1197, 1659, 2608
Tru9I TTAA 4 cut(s) 762, 1197, 1659, 2608
TscAI CASTG 5 cut(s) 1012, 1066, 1732, 1777, 2217
TseFI GTSAC 5 cut(s) 869, 979, 1286, 1768, 2198
TseI GCWGC 5 cut(s) 128, 417, 428, 1775, 1830
Tsp45I GTSAC 5 cut(s) 869, 979, 1286, 1768, 2198
TspDTI ATGAA 6 cut(s) 1241, 1488, 1672, 2145, 2183, 2232
TspGWI ACGGA 3 cut(s) 229, 1289, 1354
TspRI CASTG 5 cut(s) 1012, 1066, 1732, 1777, 2217
Tth111I GACNNNGTC 1 cut(s) 407
Vha464I CTTAAG 1 cut(s) 761
VpaK11BI GGWCC 4 cut(s) 393, 578, 774, 1539
XapI RAATTY 3 cut(s) 508, 2424, 2584
XbaI TCTAGA 1 cut(s) 1390
XmnI GAANNNNTTC 1 cut(s) 2451
XspI CTAG 9 cut(s) 438, 1391, 1602, 1808, 1964, 2012, 2148, 2394, 2459
ZraI GACGTC 2 cut(s) 217, 1416
ZrmI AGTACT 2 cut(s) 976, 1962
Zsp2I ATGCAT 1 cut(s) 1761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.