Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0011080

Physical Location & Seq

Genomic Coordinates Reverse (-)

29060737 .. 29067021

Gene Structure 6285 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG239300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2079 bp

ATGAGCAAAGCCGGGGAGGAAGAAGACGAAAACGAGTGCTTCTACGAGTCCCTCGATCGCATTCTCGTCTCCTCCTCCTCCTCCTGTTCTTGCTCCACCTCCACCTCCGACGACGACGACGACCACGACGCCGCCGTCTCTTCTTCTCCTCCCAATTATGCTTCCCGCAAGCGCTTTCCCACCCCAAAGTTCCCAATGGGGGCCTACAAGTACGACGTCTGGATCTCCGAGCCCGCCTCCGTCTCCGACCGCCGCTTACGTCTCCTCCGCGAAATGGGCCTCAGCGGCGACCCGGCCCTCTCGCGCGCCAAGCTCGACCCGGTGGGCCGCTCGGCCTCGTCGGATCATTTGACTAAACGACACAGCCCTGCCGCCGTTCCGGAAATCGTCCGGTCCAAATCGGACGGCGTCGAACACTGCAACGGCGCTGCATCCGCTAGTTCGTCGCCTATTCTTTGTTCCGAGATTGAGGCTCCGGCTCCGGCTCCGGCCGAGACTGAGACAAAAAATTCGTTTGTAAATACACAGAAAAAAAAAGATTGCGTCGCTTGTAAATCGGCGAGCGGCAAGAGCAATGGACCTTCTCCGTCGCCGCCGAATAGGCCGCCGTCCGGGAAGAACGGCCGGCGGTGCGGCGACGAGACTGGGTTAGAGGTGGAGGAGCAGGAGGAGGAGGAGGAGGAGAGTGAAGTGTGTACTATTCGGAATCTTGATAATGGGAAGGAGTTTGTGGTGAATGAGATCAGGGAAGATGGGATGTGGAACAAGCTTAAGGAAGTGGGGACCGGGAAGCAGTTGACCATGGAGGAGTTTGAGATGAGTGTGGGCCATTCCCCCATTGTTCAGGAACTGATGCGGCGGCAGAATGTGGAGGAGAGTCACAAGGCCGGTGGTGGTTCGGATTCGAATGCCAATGGGGGCGGTGGAGGGATTCCCAAGTTGAAGAAGAGGGGTTGGTTCAAGAGCATAAAGAGTGTTGCGAGTACTGTGACTGGGCATAAGGAGAGAAGGAGCAGTGATGAGAGGGACACTTCGTCGGAGAAGGGGGGCCGGAGGTCAAGCTCTGCCACTGATGATAGCCAGGATGTGTCGTTTCATGGGCCGGAGAGAGTGAGGGTCAGGCAGTATGGCAATTCTTGTAAGGAGCTGACGGCTATGTACAAGACACAGGAGATTCAGGCGCATAATGGGTCTATTTGGAGTATTAAGTTTAGCTTGGATGGGAAGTATCTGGCGAGTGCCGGTGAGGATTGTGTGATTCATATATGGCAGGTGATGGAGACGGAGAGGAAGGGTGACCTGTTGATGCAGAAATCGGAAGATAACAATTTGGATTTGTTGTTTATTGCCAATGGATCTTCAGAACCATGTTCCGTCTCGCCCAATAGTTACAGCCATCTAGAAAAAAAGAAGAGAGGGAGGACGTCTATCAGTCGGAAATCATTGAGTTTGGAACATTATGTGATTCCAGAGACTATGTTTGCACTCTCAGAAAAGCCCATCTGTTCATTCCAAGGTCATGTAGATGATGTGCTTGACCTCTCTTGGTCCAAGTCTCAGCGCCTGCTCTCATCTTCTATGGATAAAACAGTTCACCTCTGGGATTTGCCTAGCAAGTCTTGTTTGCAGAAGTTTTCACACATTGACTATGTAACTTGCATCCAGTTTAATCCCGTTGATGATAACTATTTCATTAGTGGATCACTTGATGCTAAGGTTCGTATATGGAGCATCACTGATCACCAAGTGGTTGATTGGAATGATATGCATGAGATGGTCACTGCTGCTTGCTACACTCCTGATGGCCAGGGTGCTCTAGTTGGAACCTACAAAGGGAGCTGCCGGTTATACAACACATCTGATAATAAGTTGCAAGAGAAAAGCCAAATAAATCTGCAGAACAAGAAAAAGAAATCTCATCACAAGAAAATTACTGGTTTCCAGTTTGCACCAGGAAGTTCCTCGGAAGTACTAGTTACATCTGCAGATTCACGAGTCAGGGTTGTCGATGGTATTGATCTAGTTCACAAGTTCAAGGGTAAGTATTCATTTTCTATTTTTTTGTTGTTGTTATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0010008 GO:0012505 GO:0016020 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048471 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

692

Amino Acids

76.45

Weight (kDa)

6.17

Isoelectric Point (pI)

59.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	384 - 427	4.4e-08	THOC3 beta-propeller domain
WD40	PF00400	389 - 423	9.1e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	392 - 432	1.2e-06	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	497 - 578	9.5e-13	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	498 - 624	2.6e-14	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	499 - 680	2e-25	WDR5 beta-propeller domain
WD40	PF00400	499 - 535	1.7e-07	WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd	PF25168	499 - 561	4.4e-08	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	500 - 667	3.8e-13	CDC20/Fizzy WD40 domain
EIF3I	PF24805	500 - 674	1.6e-07	EIF3I
WDR55	PF24796	501 - 629	5.1e-07	WDR55
Beta-prop_IFT122_1st	PF23381	503 - 621	3.6e-07	IFT122 first beta-propeller
Beta-prop_THOC3	PF25174	503 - 670	2.4e-18	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	504 - 583	9e-11	CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin	PF25460	506 - 628	1.4e-09	Aladin seven-bladed propeller
Beta-prop_WDR3_2nd	PF25172	510 - 668	6.9e-10	WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd	PF23393	519 - 627	7.6e-09	WDR90/POC16, second beta-propeller
Beta-prop_EML_2	PF23414	561 - 670	1e-09	Echinoderm microtubule-associated protein second beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15470 AT5G54200
fragaria_vesca	FvH4_3g20610 FvH4_3g20610
malus_domestica	MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica	Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis	pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis	RchiOBHm_Chr5g0034631
rosa_laevigata	RLG00000033560
rosa_roxburghii	Rroxscaffold_1G00045690
rosa_rugosa	Rorug05G0147300
rosa_samantha	Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana	Rw5G021930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1261
AasI	GACNNNNNNGTC	1 cut(s)	134
AatII	GACGTC	2 cut(s)	219, 1427
Acc36I	ACCTGC	1 cut(s)	1261
AccBSI	CCGCTC	2 cut(s)	330, 564
AccII	CGCG	3 cut(s)	270, 304, 306
AccIII	TCCGGA	1 cut(s)	379
AclWI	GGATC	4 cut(s)	230, 351, 1363, 1708
AcoI	YGGCCR	3 cut(s)	489, 622, 1804
AcsI	RAATTY	1 cut(s)	508
AcuI	CTGAAG	1 cut(s)	1344
AcyI	GRCGYC	4 cut(s)	129, 216, 408, 1424
AdeI	CACNNNGTG	1 cut(s)	1747
AfaI	GTAC	5 cut(s)	212, 697, 985, 1160, 1971
AfeI	AGCGCT	1 cut(s)	173
AfiI	CCNNNNNNNGG	4 cut(s)	199, 274, 611, 1833
AflII	CTTAAG	1 cut(s)	770
AgsI	TTSAA	3 cut(s)	943, 961, 2035
AhdI	GACNNNNNGTC	1 cut(s)	1033
AhlI	ACTAGT	1 cut(s)	1972
AjnI	CCWGG	3 cut(s)	1080, 1806, 1951
AluBI	AGCT	6 cut(s)	313, 769, 1062, 1147, 1215, 1839
AluI	AGCT	6 cut(s)	313, 769, 1062, 1147, 1215, 1839
Alw21I	GWGCWC	1 cut(s)	1816
AlwI	GGATC	4 cut(s)	230, 351, 1363, 1708
AlwNI	CAGNNNCTG	2 cut(s)	850, 1564
Aor13HI	TCCGGA	1 cut(s)	379
Aor51HI	AGCGCT	1 cut(s)	173
ApeKI	GCWGC	3 cut(s)	428, 1784, 1839
ApoI	RAATTY	1 cut(s)	508
ArsI	GACNNNNNNTTYG	2 cut(s)	493, 525
AspLEI	GCGC	6 cut(s)	174, 306, 308, 428, 1183, 1563
AsuC2I	CCSGG	5 cut(s)	13, 293, 320, 613, 787
AsuHPI	GGTGA	6 cut(s)	745, 1256, 1285, 1307, 1586, 1733
AsuII	TTCGAA	1 cut(s)	905
AvaII	GGWCC	4 cut(s)	393, 578, 783, 1548
BalI	TGGCCA	1 cut(s)	1806
BanII	GRGCYC	1 cut(s)	234
BauI	CACGAG	1 cut(s)	1992
BbsI	GAAGAC	1 cut(s)	30
Bbv12I	GWGCWC	1 cut(s)	1816
BbvCI	CCTCAGC	1 cut(s)	281
BbvI	GCAGC	3 cut(s)	415, 1771, 1826
BccI	CCATC	8 cut(s)	746, 1214, 1270, 1404, 1508, 1768, 1796, 2003
BceAI	ACGGC	7 cut(s)	119, 359, 421, 439, 592, 637, 1167
BciT130I	CCWGG	3 cut(s)	1082, 1808, 1953
BclI	TGATCA	1 cut(s)	1738
BcnI	CCSGG	5 cut(s)	13, 293, 320, 613, 787
BcuI	ACTAGT	1 cut(s)	1972
BfaI	CTAG	6 cut(s)	438, 1400, 1611, 1817, 1973, 2021
BfmI	CTRYAG	2 cut(s)	1895, 1983
BfoI	RGCGCY	3 cut(s)	175, 429, 1564
BfrI	CTTAAG	1 cut(s)	770
BfuAI	ACCTGC	1 cut(s)	1261
BglI	GCCNNNNNGGC	2 cut(s)	285, 601
BmcAI	AGTACT	2 cut(s)	985, 1971
Bme1390I	CCNGG	8 cut(s)	13, 293, 320, 613, 787, 1082, 1808, 1953
Bme18I	GGWCC	4 cut(s)	393, 578, 783, 1548
BmeRI	GACNNNNNGTC	1 cut(s)	1033
BmiI	GGNNCC	7 cut(s)	202, 474, 480, 486, 784, 1049, 1825
BmrFI	CCNGG	8 cut(s)	13, 293, 320, 613, 787, 1082, 1808, 1953
BmrI	ACTGGG	2 cut(s)	654, 1002
BmsI	GCATC	6 cut(s)	440, 843, 1296, 1668, 1699, 1740
BmuI	ACTGGG	2 cut(s)	654, 1002
BpiI	GAAGAC	1 cut(s)	30
BplI	GAGNNNNNCTC	2 cut(s)	221, 253
Bpu10I	CCTNAGC	2 cut(s)	281, 1713
Bpu14I	TTCGAA	1 cut(s)	905
BpuMI	CCSGG	5 cut(s)	13, 293, 320, 613, 787
BsaHI	GRCGYC	4 cut(s)	129, 216, 408, 1424
BsaJI	CCNNGG	5 cut(s)	12, 801, 1513, 1807, 1962
BsaWI	WCCGGW	2 cut(s)	379, 390
BsaXI	ACNNNNNCTCC	2 cut(s)	249, 279
Bsc4I	CCNNNNNNNGG	4 cut(s)	199, 274, 611, 1833
Bse118I	RCCGGY	4 cut(s)	624, 887, 1241, 1842
Bse1I	ACTGG	5 cut(s)	649, 997, 1663, 1939, 1942
Bse3DI	GCAATG	1 cut(s)	580
BseAI	TCCGGA	1 cut(s)	379
BseBI	CCWGG	3 cut(s)	1082, 1808, 1953
BseDI	CCNNGG	5 cut(s)	12, 801, 1513, 1807, 1962
BseGI	GGATG	5 cut(s)	431, 762, 1090, 1225, 1659
BseLI	CCNNNNNNNGG	4 cut(s)	199, 274, 611, 1833
BseMI	GCAATG	1 cut(s)	580
BseMII	CTCAG	4 cut(s)	295, 489, 1503, 1571
BseNI	ACTGG	5 cut(s)	649, 997, 1663, 1939, 1942
BsePI	GCGCGC	1 cut(s)	304
BseX3I	CGGCCG	2 cut(s)	489, 622
BseXI	GCAGC	3 cut(s)	415, 1771, 1826
Bsh1236I	CGCG	3 cut(s)	270, 304, 306
Bsh1285I	CGRYCG	4 cut(s)	58, 250, 492, 625
BsiEI	CGRYCG	4 cut(s)	58, 250, 492, 625
BsiHKAI	GWGCWC	1 cut(s)	1816
BslFI	GGGAC	3 cut(s)	34, 796, 1040
BslI	CCNNNNNNNGG	4 cut(s)	199, 274, 611, 1833
BsmBI	CGTCTC	6 cut(s)	73, 142, 247, 266, 1274, 1381
BsmFI	GGGAC	3 cut(s)	34, 796, 1040
BsmI	GAATGC	2 cut(s)	60, 913
Bsp119I	TTCGAA	1 cut(s)	905
Bsp1286I	GDGCHC	2 cut(s)	234, 1816
Bsp13I	TCCGGA	1 cut(s)	379
Bsp1407I	TGTACA	1 cut(s)	1158
Bsp143I	GATC	8 cut(s)	55, 222, 343, 741, 1355, 1700, 1738, 2017
Bsp19I	CCATGG	1 cut(s)	801
BspCNI	CTCAG	4 cut(s)	294, 490, 1502, 1570
BspEI	TCCGGA	1 cut(s)	379
BspFNI	CGCG	3 cut(s)	270, 304, 306
BspLI	GGNNCC	7 cut(s)	202, 474, 480, 486, 784, 1049, 1825
BspMAI	CTGCAG	2 cut(s)	1899, 1987
BspMI	ACCTGC	1 cut(s)	1261
BspPI	GGATC	4 cut(s)	230, 351, 1363, 1708
BspT104I	TTCGAA	1 cut(s)	905
BspTI	CTTAAG	1 cut(s)	770
BsrBI	CCGCTC	2 cut(s)	330, 564
BsrDI	GCAATG	1 cut(s)	580
BsrFI	RCCGGY	4 cut(s)	624, 887, 1241, 1842
BsrGI	TGTACA	1 cut(s)	1158
BsrI	ACTGG	5 cut(s)	649, 997, 1663, 1939, 1942
BssAI	RCCGGY	4 cut(s)	624, 887, 1241, 1842
BssECI	CCNNGG	5 cut(s)	12, 801, 1513, 1807, 1962
BssHII	GCGCGC	1 cut(s)	304
BssMI	GATC	8 cut(s)	55, 222, 343, 741, 1355, 1700, 1738, 2017
BssNI	GRCGYC	4 cut(s)	129, 216, 408, 1424
BssSI	CACGAG	1 cut(s)	1992
BssT1I	CCWWGG	2 cut(s)	801, 1513
Bst2BI	CACGAG	1 cut(s)	1992
Bst2UI	CCWGG	3 cut(s)	1082, 1808, 1953
Bst4CI	ACNGT	2 cut(s)	988, 1591
Bst6I	CTCTTC	3 cut(s)	145, 941, 1406
BstACI	GRCGYC	4 cut(s)	129, 216, 408, 1424
BstAFI	CTTAAG	1 cut(s)	770
BstAUI	TGTACA	1 cut(s)	1158
BstBI	TTCGAA	1 cut(s)	905
BstC8I	GCNNGC	7 cut(s)	170, 234, 306, 562, 626, 1565, 1789
BstDEI	CTNAG	5 cut(s)	281, 498, 1489, 1557, 1713
BstDSI	CCRYGG	1 cut(s)	801
BstEII	GGTNACC	1 cut(s)	1295
BstF5I	GGATG	5 cut(s)	431, 762, 1090, 1225, 1659
BstFNI	CGCG	3 cut(s)	270, 304, 306
BstH2I	RGCGCY	3 cut(s)	175, 429, 1564
BstHHI	GCGC	6 cut(s)	174, 306, 308, 428, 1183, 1563
BstKTI	GATC	8 cut(s)	58, 225, 346, 744, 1358, 1703, 1741, 2020
BstMBI	GATC	8 cut(s)	55, 222, 343, 741, 1355, 1700, 1738, 2017
BstMCI	CGRYCG	4 cut(s)	58, 250, 492, 625
BstMWI	GCNNNNNNNGC	7 cut(s)	276, 285, 310, 434, 570, 601, 630
BstNI	CCWGG	3 cut(s)	1082, 1808, 1953
BstPI	GGTNACC	1 cut(s)	1295
BstSCI	CCNGG	8 cut(s)	11, 291, 318, 611, 785, 1080, 1806, 1951
BstSFI	CTRYAG	2 cut(s)	1895, 1983
BstUI	CGCG	3 cut(s)	270, 304, 306
BstV1I	GCAGC	3 cut(s)	415, 1771, 1826
BstV2I	GAAGAC	1 cut(s)	30
BstX2I	RGATCY	2 cut(s)	222, 1355
BstYI	RGATCY	2 cut(s)	222, 1355
BstZI	CGGCCG	2 cut(s)	489, 622
BtgI	CCRYGG	1 cut(s)	801
BtsCI	GGATG	5 cut(s)	431, 762, 1090, 1225, 1659
BtsI	GCAGTG	3 cut(s)	415, 1021, 1779
BtsIMutI	CAGTG	5 cut(s)	415, 1021, 1068, 1734, 1779
BveI	ACCTGC	1 cut(s)	1261
Cac8I	GCNNGC	7 cut(s)	170, 234, 306, 562, 626, 1565, 1789
CaiI	CAGNNNCTG	2 cut(s)	850, 1564
CfoI	GCGC	6 cut(s)	174, 306, 308, 428, 1183, 1563
Cfr10I	RCCGGY	4 cut(s)	624, 887, 1241, 1842
CseI	GACGC	3 cut(s)	137, 397, 532
Csp6I	GTAC	5 cut(s)	211, 696, 984, 1159, 1970
CspCI	CAANNNNNGTGG	2 cut(s)	871, 906
CviAII	CATG	5 cut(s)	802, 1097, 1368, 1520, 1769
CviQI	GTAC	5 cut(s)	211, 696, 984, 1159, 1970
DdeI	CTNAG	5 cut(s)	281, 498, 1489, 1557, 1713
DpnI	GATC	8 cut(s)	57, 224, 345, 743, 1357, 1702, 1740, 2019
DpnII	GATC	8 cut(s)	55, 222, 343, 741, 1355, 1700, 1738, 2017
DraIII	CACNNNGTG	1 cut(s)	1747
DrdI	GACNNNNNNGTC	1 cut(s)	134
DriI	GACNNNNNGTC	1 cut(s)	1033
DseDI	GACNNNNNNGTC	1 cut(s)	134
EaeI	YGGCCR	3 cut(s)	489, 622, 1804
EagI	CGGCCG	2 cut(s)	489, 622
Eam1104I	CTCTTC	3 cut(s)	145, 941, 1406
Eam1105I	GACNNNNNGTC	1 cut(s)	1033
EarI	CTCTTC	3 cut(s)	145, 941, 1406
EclXI	CGGCCG	2 cut(s)	489, 622
Eco130I	CCWWGG	2 cut(s)	801, 1513
Eco24I	GRGCYC	1 cut(s)	234
Eco47I	GGWCC	4 cut(s)	393, 578, 783, 1548
Eco47III	AGCGCT	1 cut(s)	173
Eco52I	CGGCCG	2 cut(s)	489, 622
Eco57I	CTGAAG	1 cut(s)	1344
Eco91I	GGTNACC	1 cut(s)	1295
EcoO109I	RGGNCCY	1 cut(s)	201
EcoO65I	GGTNACC	1 cut(s)	1295
EcoRII	CCWGG	3 cut(s)	1080, 1806, 1951
EcoT14I	CCWWGG	2 cut(s)	801, 1513
EcoT22I	ATGCAT	1 cut(s)	1770
EcoT38I	GRGCYC	1 cut(s)	234
ErhI	CCWWGG	2 cut(s)	801, 1513
Esp3I	CGTCTC	6 cut(s)	73, 142, 247, 266, 1274, 1381
FaeI	CATG	5 cut(s)	805, 1100, 1371, 1523, 1772
FalI	AAGNNNNNCTT	2 cut(s)	1199, 1231
FaqI	GGGAC	3 cut(s)	34, 796, 1040
FatI	CATG	5 cut(s)	801, 1096, 1367, 1519, 1768
FauI	CCCGC	2 cut(s)	173, 241
FbaI	TGATCA	1 cut(s)	1738
FokI	GGATG	5 cut(s)	418, 769, 1097, 1232, 1646
FriOI	GRGCYC	1 cut(s)	234
FspBI	CTAG	6 cut(s)	438, 1400, 1611, 1817, 1973, 2021
GlaI	GCGC	6 cut(s)	173, 305, 307, 427, 1182, 1562
HaeII	RGCGCY	3 cut(s)	175, 429, 1564
HgaI	GACGC	3 cut(s)	137, 397, 532
HhaI	GCGC	6 cut(s)	174, 306, 308, 428, 1183, 1563
Hin1I	GRCGYC	4 cut(s)	129, 216, 408, 1424
Hin1II	CATG	5 cut(s)	805, 1100, 1371, 1523, 1772
Hin6I	GCGC	6 cut(s)	172, 304, 306, 426, 1181, 1561
HinP1I	GCGC	6 cut(s)	172, 304, 306, 426, 1181, 1561
HincII	GTYRAC	1 cut(s)	798
HindII	GTYRAC	1 cut(s)	798
HindIII	AAGCTT	1 cut(s)	767
HphI	GGTGA	6 cut(s)	745, 1256, 1285, 1307, 1586, 1733
Hpy166II	GTNNAC	4 cut(s)	696, 798, 1594, 2026
Hpy188III	TCNNGA	9 cut(s)	220, 380, 710, 845, 961, 1400, 1469, 1799, 1992
Hpy8I	GTNNAC	4 cut(s)	696, 798, 1594, 2026
HpyAV	CCTTC	5 cut(s)	591, 715, 1002, 1036, 1285
HpyCH4III	ACNGT	2 cut(s)	988, 1591
HpyCH4IV	ACGT	3 cut(s)	216, 259, 1424
HpyF10VI	GCNNNNNNNGC	7 cut(s)	276, 285, 310, 434, 570, 601, 630
HpyF3I	CTNAG	5 cut(s)	281, 498, 1489, 1557, 1713
HpySE526I	ACGT	3 cut(s)	216, 259, 1424
Hsp92I	GRCGYC	4 cut(s)	129, 216, 408, 1424
Hsp92II	CATG	5 cut(s)	805, 1100, 1371, 1523, 1772
HspAI	GCGC	6 cut(s)	172, 304, 306, 426, 1181, 1561
Kpn2I	TCCGGA	1 cut(s)	379
KroI	GCCGGC	1 cut(s)	624
KroNI	GCCGGC	1 cut(s)	626
Ksp22I	TGATCA	1 cut(s)	1738
Kzo9I	GATC	8 cut(s)	55, 222, 343, 741, 1355, 1700, 1738, 2017
LmnI	GCTCC	9 cut(s)	98, 478, 484, 490, 661, 1011, 1144, 1728, 1836
Lsp1109I	GCAGC	3 cut(s)	415, 1771, 1826
LweI	GCATC	6 cut(s)	440, 843, 1296, 1668, 1699, 1740
MaeI	CTAG	6 cut(s)	438, 1400, 1611, 1817, 1973, 2021
MaeII	ACGT	3 cut(s)	216, 259, 1424
MaeIII	GTNAC	7 cut(s)	878, 988, 1295, 1388, 1651, 1777, 1975
MalI	GATC	8 cut(s)	57, 224, 345, 743, 1357, 1702, 1740, 2019
MbiI	CCGCTC	2 cut(s)	330, 564
MboI	GATC	8 cut(s)	55, 222, 343, 741, 1355, 1700, 1738, 2017
MflI	RGATCY	2 cut(s)	222, 1355
MhlI	GDGCHC	2 cut(s)	234, 1816
MlsI	TGGCCA	1 cut(s)	1806
MluCI	AATT	5 cut(s)	154, 508, 1132, 1327, 1929
MluNI	TGGCCA	1 cut(s)	1806
MlyI	GAGTC	3 cut(s)	56, 886, 2004
MmeI	TCCRAC	6 cut(s)	132, 270, 321, 1017, 1415, 1801
Mox20I	TGGCCA	1 cut(s)	1806
Mph1103I	ATGCAT	1 cut(s)	1770
MroI	TCCGGA	1 cut(s)	379
MroNI	GCCGGC	1 cut(s)	624
MscI	TGGCCA	1 cut(s)	1806
MseI	TTAA	3 cut(s)	771, 1206, 1668
MslI	CAYNNNNRTG	1 cut(s)	1524
Msp20I	TGGCCA	1 cut(s)	1806
MspA1I	CMGCKG	1 cut(s)	285
MspCI	CTTAAG	1 cut(s)	770
MspR9I	CCNGG	8 cut(s)	13, 293, 320, 613, 787, 1082, 1808, 1953
Mva1269I	GAATGC	2 cut(s)	60, 913
MvaI	CCWGG	3 cut(s)	1082, 1808, 1953
MvnI	CGCG	3 cut(s)	270, 304, 306
MwoI	GCNNNNNNNGC	7 cut(s)	276, 285, 310, 434, 570, 601, 630
NaeI	GCCGGC	1 cut(s)	626
NciI	CCSGG	5 cut(s)	13, 293, 320, 613, 787
NcoI	CCATGG	1 cut(s)	801
NdeII	GATC	8 cut(s)	55, 222, 343, 741, 1355, 1700, 1738, 2017
NgoMIV	GCCGGC	1 cut(s)	624
NlaIII	CATG	5 cut(s)	805, 1100, 1371, 1523, 1772
NlaIV	GGNNCC	7 cut(s)	202, 474, 480, 486, 784, 1049, 1825
NmeAIII	GCCGAG	2 cut(s)	311, 517
NmuCI	GTSAC	4 cut(s)	878, 988, 1295, 1777
NsiI	ATGCAT	1 cut(s)	1770
NspV	TTCGAA	1 cut(s)	905
PaqCI	CACCTGC	1 cut(s)	1261
PauI	GCGCGC	1 cut(s)	304
PcsI	WCGNNNNNNNCGW	4 cut(s)	51, 117, 123, 132
PctI	GAATGC	2 cut(s)	60, 913
PdiI	GCCGGC	1 cut(s)	626
PfeI	GAWTC	7 cut(s)	706, 902, 931, 1174, 1258, 1465, 1988
PflFI	GACNNNGTC	1 cut(s)	407
PfoI	TCCNGGA	1 cut(s)	611
Ple19I	CGATCG	1 cut(s)	58
PleI	GAGTC	3 cut(s)	55, 885, 2003
PpsI	GAGTC	3 cut(s)	55, 885, 2003
Psp6I	CCWGG	3 cut(s)	1080, 1806, 1951
PspEI	GGTNACC	1 cut(s)	1295
PspGI	CCWGG	3 cut(s)	1080, 1806, 1951
PspN4I	GGNNCC	7 cut(s)	202, 474, 480, 486, 784, 1049, 1825
PstI	CTGCAG	2 cut(s)	1899, 1987
PstNI	CAGNNNCTG	2 cut(s)	850, 1564
PsuI	RGATCY	2 cut(s)	222, 1355
PsyI	GACNNNGTC	1 cut(s)	407
PteI	GCGCGC	1 cut(s)	304
PvuI	CGATCG	1 cut(s)	58
RsaI	GTAC	5 cut(s)	212, 697, 985, 1160, 1971
RsaNI	GTAC	5 cut(s)	211, 696, 984, 1159, 1970
RseI	CAYNNNNRTG	1 cut(s)	1524
SaqAI	TTAA	3 cut(s)	771, 1206, 1668
Sau3AI	GATC	8 cut(s)	55, 222, 343, 741, 1355, 1700, 1738, 2017
ScaI	AGTACT	2 cut(s)	985, 1971
SchI	GAGTC	3 cut(s)	56, 886, 2004
ScrFI	CCNGG	8 cut(s)	13, 293, 320, 613, 787, 1082, 1808, 1953
SduI	GDGCHC	2 cut(s)	234, 1816
SfaNI	GCATC	6 cut(s)	440, 843, 1296, 1668, 1699, 1740
SfcI	CTRYAG	2 cut(s)	1895, 1983
SfuI	TTCGAA	1 cut(s)	905
SinI	GGWCC	4 cut(s)	393, 578, 783, 1548
SmiMI	CAYNNNNRTG	1 cut(s)	1524
SmlI	CTYRAG	1 cut(s)	770
SmoI	CTYRAG	1 cut(s)	770
SpeI	ACTAGT	1 cut(s)	1972
Sse9I	AATT	5 cut(s)	154, 508, 1132, 1327, 1929
SspMI	CTAG	6 cut(s)	438, 1400, 1611, 1817, 1973, 2021
StyD4I	CCNGG	8 cut(s)	11, 291, 318, 611, 785, 1080, 1806, 1951
StyI	CCWWGG	2 cut(s)	801, 1513
TaaI	ACNGT	2 cut(s)	988, 1591
TaiI	ACGT	3 cut(s)	219, 262, 1427
TaqI	TCGA	5 cut(s)	54, 315, 411, 905, 2007
TasI	AATT	5 cut(s)	154, 508, 1132, 1327, 1929
TatI	WGTACW	4 cut(s)	695, 983, 1158, 1969
TfiI	GAWTC	7 cut(s)	706, 902, 931, 1174, 1258, 1465, 1988
Tru1I	TTAA	3 cut(s)	771, 1206, 1668
Tru9I	TTAA	3 cut(s)	771, 1206, 1668
TscAI	CASTG	5 cut(s)	422, 1021, 1075, 1741, 1786
TseFI	GTSAC	4 cut(s)	878, 988, 1295, 1777
TseI	GCWGC	3 cut(s)	428, 1784, 1839
Tsp45I	GTSAC	4 cut(s)	878, 988, 1295, 1777
TspDTI	ATGAA	5 cut(s)	1085, 1250, 1497, 1681, 2037
TspGWI	ACGGA	4 cut(s)	229, 576, 1298, 1363
TspRI	CASTG	5 cut(s)	422, 1021, 1075, 1741, 1786
Tth111I	GACNNNGTC	1 cut(s)	407
Vha464I	CTTAAG	1 cut(s)	770
VpaK11BI	GGWCC	4 cut(s)	393, 578, 783, 1548
XapI	RAATTY	1 cut(s)	508
XbaI	TCTAGA	1 cut(s)	1399
XspI	CTAG	6 cut(s)	438, 1400, 1611, 1817, 1973, 2021
ZraI	GACGTC	2 cut(s)	217, 1425
ZrmI	AGTACT	2 cut(s)	985, 1971
Zsp2I	ATGCAT	1 cut(s)	1770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.