Rh5DG248200

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011080
Physical Location & Seq
Reverse (-)
28459497 .. 28465744
6248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG248200.1

Sequence Viewer

Length: 2073 bp
ATGAGCAAAGCCGGGGAGGAAGAAGACGAAGACGAGTGCTTCTACGAGTCCCTCGATCGCATTCTCGTCTCCTCCTCCTCCTCCTCCTGTTCTTGCTCCACCTCCACCTCCGACGACGACGACGCCGCCGCCGCCGTCTCTTCTTCTCCTCCCAATTATGCTTCCCGCAAGCGCTTTCCCACCCCAAAGTTCCCAATGGGGGCCTACAAGTACGACGTCTGGATCTCCGAGCCCGCCTCCGTCTCCGACCGCCGCTTACGTCTCCTCCGCGAAATGGGCCTCAGCGGCGACCCGGCCCTCTCGCGCGCCAAGCCCGACCCGGTGGGCCGCTCGGCCTCGTCGGATCATTTGACTAAACGACACAGCCCTGCCGCCGTTCCGGAAATCGTCCGGTCCAAATCGGACGGCGTCGAACGCTGCAACGGCGCTGCATCCGCTAGTTCGTCGCCTATTCTTTGTTCCGAGATTGAGGCTCCGGCTCCGGCCGAGACCGAGACCGAGACCAAAAATTCGTTTGTAAATACACAGAAAAAAAAAGATTGCGTCGTTTGTAAATCGGCGAGCGGCAAGAGCAATGGACCTTCGCCGTCGCCGCCGAATAGGCCGCCGTCCGGGAAGAACGGCCGGCGGTGCGGCGACGAGATTGGGTTAGAGGTGGAGGAGGAGGAGGAGGAGGAGAGTGAAGTGTGTACTATTCGGAATCTTGATAATGGGAAGGAGTTTGTGGTGAATGAGATCAGGGAAGATGGGATGTGGAACAAGCTTAAGGAAGTGGGGACCGGGAAGCAGTTGACCATGGAGGAGTTTGAGATGAGTGTGGGCCATTCCCCCATTGTTCAGGAACTGATGCGGCGGCAGAATGTGGAGGAGAGTCACAAGGCCGGTGGTGGTTCGGATTCGAATGCCAATGGGGGCGGTGGAGGGATTCCCAAGTTGAAGAAGAGGGGTTGGTTCAAGAGCATAAAGAGTGTTGCCAGTACTGTGACTGGGCATAAGGAGAGAAGGAGCAGTGATGAGAGGGACACTTCGTCGGAGAAGGGGGGCCGGAGGTCAAGCTCTGCCACTGATGATAGCCAGGATGTGACGTTTCATGGGCCGGAGAGAGTGAGGGTCAGGCAGTATGGCAAGTCTTGTAAGGAGCTGACGGCTATGTACAAGACACAGGAGATTCAGGCGCATAATGGGTCTATTTGGAGTATTAAGTTTAGCTTGGATGGGAAGTATCTGGCGAGTGCCGGTGAGGATTGTGTGATTCATATATGGCAGGTGATGGAGACGGAGAGGAAGGGTGACCTGTTGATGCAGAAATCGGAAGATAACAATTTGGATTTGTTGTTTATTGCCAATGGATCTTCAGAACCATGTTCCGTCTCGCCCAATAGTTACAGCCATCTAGAAAAAAAGAAGAGAGGGAGGACGTCTATCAGTCGGAAATCATTGAGTTTGGAACATTATGTGATTCCAGAGACTATGTTTGCACTCTCAGAAAAGCCCATCTGTTCATTCCAAGGTCATGTAGATGATGTGCTTGACCTCTCTTGGTCCAAGTCTCAGCGCCTGCTCTCATCTTCTATGGATAAAACGGTTCACCTCTGGGATTTGCCTAGCAAGTCTTGTTTGCAGAAGTTTTCACACATTGACTATGTAACTTGCATCCAGTTTAATCCCGTTGATGATAACTACTTCATTAGTGGATCACTTGATGCTAAGGTTCGTATATGGAGTATCACTGATCGCCAAGTGGTTGATTGGAATGATATGCATGAGATGGTCACTGCTGCTTGCTACACTCCTGATGGCCAGGGTGCTCTAGTTGGAACCTACAAAGGGAGTTGCCGGTTATACAACACATCTGATAATAAGTTGCAAGAGAAAAGCCAAATAAATCTGCAGAACAAGAAAAAGAAATCTCATCACAAGAAAATTACTGGTTTCCAGTTTGCACCAGGAAGTTCCTCGGAAGTACTAGTTACATCTGCAGATTCACGAGTCAGGGTTGTCGATGGTATTGATCTAGTTCACAAGTTCAAGGGTAAGTATTCATTTTCTATTTTTTTGTTGTTGTTGTTTTAG

Protein Analysis

690

Amino Acids

76.21

Weight (kDa)

6.42

Isoelectric Point (pI)

58.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 382 - 425 4.5e-08 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 384 - 428 9.8e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 387 - 421 9e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 390 - 430 1.2e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 495 - 576 9.5e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 496 - 622 2.7e-14 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 497 - 678 1.2e-25 WDR5 beta-propeller domain
WD40 PF00400 497 - 533 1.7e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 497 - 559 4.4e-08 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 498 - 665 3.1e-13 CDC20/Fizzy WD40 domain
EIF3I PF24805 498 - 672 1.7e-07 EIF3I
WDR55 PF24796 499 - 627 5.2e-07 WDR55
Beta-prop_IFT122_1st PF23381 501 - 619 2.2e-07 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 502 - 581 9.4e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 504 - 626 2.8e-09 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 505 - 669 7.5e-18 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 508 - 666 8e-10 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 517 - 625 8.2e-09 WDR90/POC16, second beta-propeller
Beta-prop_EML_2 PF23414 559 - 668 2.2e-09 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15470 AT5G54200
fragaria_vesca FvH4_3g20610 FvH4_3g20610
malus_domestica MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis RchiOBHm_Chr5g0034631
rosa_laevigata RLG00000033560
rosa_roxburghii Rroxscaffold_1G00045690
rosa_rugosa Rorug05G0147300
rosa_samantha Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana Rw5G021930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1255
AatII GACGTC 2 cut(s) 219, 1421
Acc36I ACCTGC 1 cut(s) 1255
AccBSI CCGCTC 2 cut(s) 330, 564
AccII CGCG 3 cut(s) 270, 304, 306
AccIII TCCGGA 1 cut(s) 379
AclWI GGATC 4 cut(s) 230, 351, 1357, 1702
AcoI YGGCCR 3 cut(s) 483, 622, 1798
AcsI RAATTY 1 cut(s) 508
AcuI CTGAAG 1 cut(s) 1338
AcyI GRCGYC 4 cut(s) 123, 216, 408, 1418
AfaI GTAC 5 cut(s) 212, 691, 979, 1154, 1965
AfeI AGCGCT 1 cut(s) 173
AfiI CCNNNNNNNGG 4 cut(s) 199, 274, 611, 1827
AflII CTTAAG 1 cut(s) 764
AgsI TTSAA 3 cut(s) 937, 955, 2029
AhdI GACNNNNNGTC 1 cut(s) 1027
AhlI ACTAGT 1 cut(s) 1966
AjnI CCWGG 3 cut(s) 1074, 1800, 1945
AluBI AGCT 4 cut(s) 763, 1056, 1141, 1209
AluI AGCT 4 cut(s) 763, 1056, 1141, 1209
Alw21I GWGCWC 1 cut(s) 1810
AlwI GGATC 4 cut(s) 230, 351, 1357, 1702
AlwNI CAGNNNCTG 2 cut(s) 844, 1558
Aor13HI TCCGGA 1 cut(s) 379
Aor51HI AGCGCT 1 cut(s) 173
ApeKI GCWGC 3 cut(s) 417, 428, 1778
ApoI RAATTY 1 cut(s) 508
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
AspLEI GCGC 6 cut(s) 174, 306, 308, 428, 1177, 1557
AsuC2I CCSGG 5 cut(s) 13, 293, 320, 613, 781
AsuHPI GGTGA 5 cut(s) 739, 1250, 1279, 1301, 1580
AsuII TTCGAA 1 cut(s) 899
AvaII GGWCC 4 cut(s) 393, 578, 777, 1542
BalI TGGCCA 1 cut(s) 1800
BanII GRGCYC 1 cut(s) 234
BauI CACGAG 1 cut(s) 1986
BbsI GAAGAC 2 cut(s) 30, 36
Bbv12I GWGCWC 1 cut(s) 1810
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 3 cut(s) 404, 415, 1765
BccI CCATC 8 cut(s) 740, 1208, 1264, 1398, 1502, 1762, 1790, 1997
BceAI ACGGC 8 cut(s) 119, 359, 421, 439, 571, 592, 637, 1161
BciT130I CCWGG 3 cut(s) 1076, 1802, 1947
BcnI CCSGG 5 cut(s) 13, 293, 320, 613, 781
BcuI ACTAGT 1 cut(s) 1966
BfaI CTAG 6 cut(s) 438, 1394, 1605, 1811, 1967, 2015
BfmI CTRYAG 2 cut(s) 1889, 1977
BfoI RGCGCY 3 cut(s) 175, 429, 1558
BfrI CTTAAG 1 cut(s) 764
BfuAI ACCTGC 1 cut(s) 1255
BglI GCCNNNNNGGC 2 cut(s) 285, 601
BmcAI AGTACT 2 cut(s) 979, 1965
Bme1390I CCNGG 8 cut(s) 13, 293, 320, 613, 781, 1076, 1802, 1947
Bme18I GGWCC 4 cut(s) 393, 578, 777, 1542
BmeRI GACNNNNNGTC 1 cut(s) 1027
BmiI GGNNCC 6 cut(s) 202, 474, 480, 778, 1043, 1819
BmrFI CCNGG 8 cut(s) 13, 293, 320, 613, 781, 1076, 1802, 1947
BmrI ACTGGG 1 cut(s) 996
BmsI GCATC 5 cut(s) 440, 837, 1290, 1662, 1693
BmuI ACTGGG 1 cut(s) 996
BpiI GAAGAC 2 cut(s) 30, 36
BplI GAGNNNNNCTC 2 cut(s) 221, 253
Bpu10I CCTNAGC 2 cut(s) 281, 1707
Bpu14I TTCGAA 1 cut(s) 899
BpuMI CCSGG 5 cut(s) 13, 293, 320, 613, 781
BsaHI GRCGYC 4 cut(s) 123, 216, 408, 1418
BsaI GGTCTC 3 cut(s) 482, 488, 494
BsaJI CCNNGG 5 cut(s) 12, 795, 1507, 1801, 1956
BsaWI WCCGGW 2 cut(s) 379, 390
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 274, 611, 1827
Bse118I RCCGGY 4 cut(s) 624, 881, 1235, 1836
Bse1I ACTGG 5 cut(s) 975, 991, 1657, 1933, 1936
Bse3DI GCAATG 1 cut(s) 580
BseAI TCCGGA 1 cut(s) 379
BseBI CCWGG 3 cut(s) 1076, 1802, 1947
BseDI CCNNGG 5 cut(s) 12, 795, 1507, 1801, 1956
BseGI GGATG 5 cut(s) 431, 756, 1084, 1219, 1653
BseLI CCNNNNNNNGG 4 cut(s) 199, 274, 611, 1827
BseMI GCAATG 1 cut(s) 580
BseMII CTCAG 3 cut(s) 295, 1497, 1565
BseNI ACTGG 5 cut(s) 975, 991, 1657, 1933, 1936
BsePI GCGCGC 1 cut(s) 304
BseX3I CGGCCG 2 cut(s) 483, 622
BseXI GCAGC 3 cut(s) 404, 415, 1765
Bsh1236I CGCG 3 cut(s) 270, 304, 306
Bsh1285I CGRYCG 4 cut(s) 58, 250, 486, 625
BsiEI CGRYCG 4 cut(s) 58, 250, 486, 625
BsiHKAI GWGCWC 1 cut(s) 1810
BslFI GGGAC 3 cut(s) 34, 790, 1034
BslI CCNNNNNNNGG 4 cut(s) 199, 274, 611, 1827
BsmBI CGTCTC 6 cut(s) 73, 142, 247, 266, 1268, 1375
BsmFI GGGAC 3 cut(s) 34, 790, 1034
BsmI GAATGC 2 cut(s) 60, 907
Bso31I GGTCTC 3 cut(s) 482, 488, 494
Bsp119I TTCGAA 1 cut(s) 899
Bsp1286I GDGCHC 2 cut(s) 234, 1810
Bsp13I TCCGGA 1 cut(s) 379
Bsp1407I TGTACA 1 cut(s) 1152
Bsp143I GATC 8 cut(s) 55, 222, 343, 735, 1349, 1694, 1732, 2011
Bsp19I CCATGG 1 cut(s) 795
BspCNI CTCAG 3 cut(s) 294, 1496, 1564
BspEI TCCGGA 1 cut(s) 379
BspFNI CGCG 3 cut(s) 270, 304, 306
BspLI GGNNCC 6 cut(s) 202, 474, 480, 778, 1043, 1819
BspMAI CTGCAG 2 cut(s) 1893, 1981
BspMI ACCTGC 1 cut(s) 1255
BspPI GGATC 4 cut(s) 230, 351, 1357, 1702
BspT104I TTCGAA 1 cut(s) 899
BspTI CTTAAG 1 cut(s) 764
BspTNI GGTCTC 3 cut(s) 482, 488, 494
BsrBI CCGCTC 2 cut(s) 330, 564
BsrDI GCAATG 1 cut(s) 580
BsrFI RCCGGY 4 cut(s) 624, 881, 1235, 1836
BsrGI TGTACA 1 cut(s) 1152
BsrI ACTGG 5 cut(s) 975, 991, 1657, 1933, 1936
BssAI RCCGGY 4 cut(s) 624, 881, 1235, 1836
BssECI CCNNGG 5 cut(s) 12, 795, 1507, 1801, 1956
BssHII GCGCGC 1 cut(s) 304
BssMI GATC 8 cut(s) 55, 222, 343, 735, 1349, 1694, 1732, 2011
BssNI GRCGYC 4 cut(s) 123, 216, 408, 1418
BssSI CACGAG 1 cut(s) 1986
BssT1I CCWWGG 2 cut(s) 795, 1507
Bst2BI CACGAG 1 cut(s) 1986
Bst2UI CCWGG 3 cut(s) 1076, 1802, 1947
Bst4CI ACNGT 2 cut(s) 982, 1585
Bst6I CTCTTC 3 cut(s) 145, 935, 1400
BstACI GRCGYC 4 cut(s) 123, 216, 408, 1418
BstAFI CTTAAG 1 cut(s) 764
BstAUI TGTACA 1 cut(s) 1152
BstBI TTCGAA 1 cut(s) 899
BstC8I GCNNGC 7 cut(s) 170, 234, 306, 562, 626, 1559, 1783
BstDEI CTNAG 4 cut(s) 281, 1483, 1551, 1707
BstDSI CCRYGG 1 cut(s) 795
BstEII GGTNACC 1 cut(s) 1289
BstF5I GGATG 5 cut(s) 431, 756, 1084, 1219, 1653
BstFNI CGCG 3 cut(s) 270, 304, 306
BstH2I RGCGCY 3 cut(s) 175, 429, 1558
BstHHI GCGC 6 cut(s) 174, 306, 308, 428, 1177, 1557
BstKTI GATC 8 cut(s) 58, 225, 346, 738, 1352, 1697, 1735, 2014
BstMBI GATC 8 cut(s) 55, 222, 343, 735, 1349, 1694, 1732, 2011
BstMCI CGRYCG 4 cut(s) 58, 250, 486, 625
BstNI CCWGG 3 cut(s) 1076, 1802, 1947
BstPI GGTNACC 1 cut(s) 1289
BstSCI CCNGG 8 cut(s) 11, 291, 318, 611, 779, 1074, 1800, 1945
BstSFI CTRYAG 2 cut(s) 1889, 1977
BstUI CGCG 3 cut(s) 270, 304, 306
BstV1I GCAGC 3 cut(s) 404, 415, 1765
BstV2I GAAGAC 2 cut(s) 30, 36
BstX2I RGATCY 2 cut(s) 222, 1349
BstYI RGATCY 2 cut(s) 222, 1349
BstZI CGGCCG 2 cut(s) 483, 622
BtgI CCRYGG 1 cut(s) 795
BtsCI GGATG 5 cut(s) 431, 756, 1084, 1219, 1653
BtsI GCAGTG 2 cut(s) 1015, 1773
BtsIMutI CAGTG 4 cut(s) 1015, 1062, 1728, 1773
BveI ACCTGC 1 cut(s) 1255
Cac8I GCNNGC 7 cut(s) 170, 234, 306, 562, 626, 1559, 1783
CaiI CAGNNNCTG 2 cut(s) 844, 1558
CfoI GCGC 6 cut(s) 174, 306, 308, 428, 1177, 1557
Cfr10I RCCGGY 4 cut(s) 624, 881, 1235, 1836
CseI GACGC 3 cut(s) 131, 397, 532
Csp6I GTAC 5 cut(s) 211, 690, 978, 1153, 1964
CspCI CAANNNNNGTGG 2 cut(s) 865, 900
CviAII CATG 5 cut(s) 796, 1091, 1362, 1514, 1763
CviQI GTAC 5 cut(s) 211, 690, 978, 1153, 1964
DdeI CTNAG 4 cut(s) 281, 1483, 1551, 1707
DpnI GATC 8 cut(s) 57, 224, 345, 737, 1351, 1696, 1734, 2013
DpnII GATC 8 cut(s) 55, 222, 343, 735, 1349, 1694, 1732, 2011
DriI GACNNNNNGTC 1 cut(s) 1027
EaeI YGGCCR 3 cut(s) 483, 622, 1798
EagI CGGCCG 2 cut(s) 483, 622
Eam1104I CTCTTC 3 cut(s) 145, 935, 1400
Eam1105I GACNNNNNGTC 1 cut(s) 1027
EarI CTCTTC 3 cut(s) 145, 935, 1400
EclXI CGGCCG 2 cut(s) 483, 622
Eco130I CCWWGG 2 cut(s) 795, 1507
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 3 cut(s) 482, 488, 494
Eco47I GGWCC 4 cut(s) 393, 578, 777, 1542
Eco47III AGCGCT 1 cut(s) 173
Eco52I CGGCCG 2 cut(s) 483, 622
Eco57I CTGAAG 1 cut(s) 1338
Eco91I GGTNACC 1 cut(s) 1289
EcoO109I RGGNCCY 1 cut(s) 201
EcoO65I GGTNACC 1 cut(s) 1289
EcoRII CCWGG 3 cut(s) 1074, 1800, 1945
EcoT14I CCWWGG 2 cut(s) 795, 1507
EcoT22I ATGCAT 1 cut(s) 1764
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 2 cut(s) 795, 1507
Esp3I CGTCTC 6 cut(s) 73, 142, 247, 266, 1268, 1375
FaeI CATG 5 cut(s) 799, 1094, 1365, 1517, 1766
FalI AAGNNNNNCTT 2 cut(s) 1193, 1225
FaqI GGGAC 3 cut(s) 34, 790, 1034
FatI CATG 5 cut(s) 795, 1090, 1361, 1513, 1762
FauI CCCGC 2 cut(s) 173, 241
FokI GGATG 5 cut(s) 418, 763, 1091, 1226, 1640
FriOI GRGCYC 1 cut(s) 234
FspBI CTAG 6 cut(s) 438, 1394, 1605, 1811, 1967, 2015
GlaI GCGC 6 cut(s) 173, 305, 307, 427, 1176, 1556
HaeII RGCGCY 3 cut(s) 175, 429, 1558
HgaI GACGC 3 cut(s) 131, 397, 532
HhaI GCGC 6 cut(s) 174, 306, 308, 428, 1177, 1557
Hin1I GRCGYC 4 cut(s) 123, 216, 408, 1418
Hin1II CATG 5 cut(s) 799, 1094, 1365, 1517, 1766
Hin6I GCGC 6 cut(s) 172, 304, 306, 426, 1175, 1555
HinP1I GCGC 6 cut(s) 172, 304, 306, 426, 1175, 1555
HincII GTYRAC 1 cut(s) 792
HindII GTYRAC 1 cut(s) 792
HindIII AAGCTT 1 cut(s) 761
HphI GGTGA 5 cut(s) 739, 1250, 1279, 1301, 1580
Hpy166II GTNNAC 4 cut(s) 690, 792, 1588, 2020
Hpy188III TCNNGA 9 cut(s) 220, 380, 704, 839, 955, 1394, 1463, 1793, 1986
Hpy8I GTNNAC 4 cut(s) 690, 792, 1588, 2020
HpyAV CCTTC 5 cut(s) 591, 709, 996, 1030, 1279
HpyCH4III ACNGT 2 cut(s) 982, 1585
HpyCH4IV ACGT 4 cut(s) 216, 259, 1085, 1418
HpyF3I CTNAG 4 cut(s) 281, 1483, 1551, 1707
HpySE526I ACGT 4 cut(s) 216, 259, 1085, 1418
Hsp92I GRCGYC 4 cut(s) 123, 216, 408, 1418
Hsp92II CATG 5 cut(s) 799, 1094, 1365, 1517, 1766
HspAI GCGC 6 cut(s) 172, 304, 306, 426, 1175, 1555
Kpn2I TCCGGA 1 cut(s) 379
KroI GCCGGC 1 cut(s) 624
KroNI GCCGGC 1 cut(s) 626
Kzo9I GATC 8 cut(s) 55, 222, 343, 735, 1349, 1694, 1732, 2011
LmnI GCTCC 5 cut(s) 101, 478, 484, 1005, 1138
Lsp1109I GCAGC 3 cut(s) 404, 415, 1765
LweI GCATC 5 cut(s) 440, 837, 1290, 1662, 1693
MaeI CTAG 6 cut(s) 438, 1394, 1605, 1811, 1967, 2015
MaeII ACGT 4 cut(s) 216, 259, 1085, 1418
MaeIII GTNAC 8 cut(s) 872, 982, 1081, 1289, 1382, 1645, 1771, 1969
MalI GATC 8 cut(s) 57, 224, 345, 737, 1351, 1696, 1734, 2013
MbiI CCGCTC 2 cut(s) 330, 564
MboI GATC 8 cut(s) 55, 222, 343, 735, 1349, 1694, 1732, 2011
MflI RGATCY 2 cut(s) 222, 1349
MhlI GDGCHC 2 cut(s) 234, 1810
MlsI TGGCCA 1 cut(s) 1800
MluCI AATT 4 cut(s) 154, 508, 1321, 1923
MluNI TGGCCA 1 cut(s) 1800
MlyI GAGTC 3 cut(s) 56, 880, 1998
MmeI TCCRAC 6 cut(s) 135, 270, 321, 1011, 1409, 1795
Mox20I TGGCCA 1 cut(s) 1800
Mph1103I ATGCAT 1 cut(s) 1764
MroI TCCGGA 1 cut(s) 379
MroNI GCCGGC 1 cut(s) 624
MscI TGGCCA 1 cut(s) 1800
MseI TTAA 3 cut(s) 765, 1200, 1662
MslI CAYNNNNRTG 1 cut(s) 1518
Msp20I TGGCCA 1 cut(s) 1800
MspA1I CMGCKG 1 cut(s) 285
MspCI CTTAAG 1 cut(s) 764
MspR9I CCNGG 8 cut(s) 13, 293, 320, 613, 781, 1076, 1802, 1947
Mva1269I GAATGC 2 cut(s) 60, 907
MvaI CCWGG 3 cut(s) 1076, 1802, 1947
MvnI CGCG 3 cut(s) 270, 304, 306
NaeI GCCGGC 1 cut(s) 626
NciI CCSGG 5 cut(s) 13, 293, 320, 613, 781
NcoI CCATGG 1 cut(s) 795
NdeII GATC 8 cut(s) 55, 222, 343, 735, 1349, 1694, 1732, 2011
NgoMIV GCCGGC 1 cut(s) 624
NlaIII CATG 5 cut(s) 799, 1094, 1365, 1517, 1766
NlaIV GGNNCC 6 cut(s) 202, 474, 480, 778, 1043, 1819
NmeAIII GCCGAG 2 cut(s) 311, 511
NmuCI GTSAC 5 cut(s) 872, 982, 1081, 1289, 1771
NsiI ATGCAT 1 cut(s) 1764
NspV TTCGAA 1 cut(s) 899
PaqCI CACCTGC 1 cut(s) 1255
PauI GCGCGC 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 2 cut(s) 60, 907
PdiI GCCGGC 1 cut(s) 626
PfeI GAWTC 7 cut(s) 700, 896, 925, 1168, 1252, 1459, 1982
PflFI GACNNNGTC 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 611
Ple19I CGATCG 1 cut(s) 58
PleI GAGTC 3 cut(s) 55, 879, 1997
PpsI GAGTC 3 cut(s) 55, 879, 1997
Psp6I CCWGG 3 cut(s) 1074, 1800, 1945
PspEI GGTNACC 1 cut(s) 1289
PspGI CCWGG 3 cut(s) 1074, 1800, 1945
PspN4I GGNNCC 6 cut(s) 202, 474, 480, 778, 1043, 1819
PstI CTGCAG 2 cut(s) 1893, 1981
PstNI CAGNNNCTG 2 cut(s) 844, 1558
PsuI RGATCY 2 cut(s) 222, 1349
PsyI GACNNNGTC 1 cut(s) 407
PteI GCGCGC 1 cut(s) 304
PvuI CGATCG 1 cut(s) 58
RsaI GTAC 5 cut(s) 212, 691, 979, 1154, 1965
RsaNI GTAC 5 cut(s) 211, 690, 978, 1153, 1964
RseI CAYNNNNRTG 1 cut(s) 1518
SaqAI TTAA 3 cut(s) 765, 1200, 1662
Sau3AI GATC 8 cut(s) 55, 222, 343, 735, 1349, 1694, 1732, 2011
ScaI AGTACT 2 cut(s) 979, 1965
SchI GAGTC 3 cut(s) 56, 880, 1998
ScrFI CCNGG 8 cut(s) 13, 293, 320, 613, 781, 1076, 1802, 1947
SduI GDGCHC 2 cut(s) 234, 1810
SfaNI GCATC 5 cut(s) 440, 837, 1290, 1662, 1693
SfcI CTRYAG 2 cut(s) 1889, 1977
SfuI TTCGAA 1 cut(s) 899
SinI GGWCC 4 cut(s) 393, 578, 777, 1542
SmiMI CAYNNNNRTG 1 cut(s) 1518
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
SpeI ACTAGT 1 cut(s) 1966
Sse9I AATT 4 cut(s) 154, 508, 1321, 1923
SspMI CTAG 6 cut(s) 438, 1394, 1605, 1811, 1967, 2015
StyD4I CCNGG 8 cut(s) 11, 291, 318, 611, 779, 1074, 1800, 1945
StyI CCWWGG 2 cut(s) 795, 1507
TaaI ACNGT 2 cut(s) 982, 1585
TaiI ACGT 4 cut(s) 219, 262, 1088, 1421
TaqI TCGA 4 cut(s) 54, 411, 899, 2001
TaqII GACCGA 2 cut(s) 506, 512
TasI AATT 4 cut(s) 154, 508, 1321, 1923
TatI WGTACW 4 cut(s) 689, 977, 1152, 1963
TfiI GAWTC 7 cut(s) 700, 896, 925, 1168, 1252, 1459, 1982
Tru1I TTAA 3 cut(s) 765, 1200, 1662
Tru9I TTAA 3 cut(s) 765, 1200, 1662
TscAI CASTG 4 cut(s) 1015, 1069, 1735, 1780
TseFI GTSAC 5 cut(s) 872, 982, 1081, 1289, 1771
TseI GCWGC 3 cut(s) 417, 428, 1778
Tsp45I GTSAC 5 cut(s) 872, 982, 1081, 1289, 1771
TspDTI ATGAA 5 cut(s) 1079, 1244, 1491, 1675, 2031
TspGWI ACGGA 3 cut(s) 229, 1292, 1357
TspRI CASTG 4 cut(s) 1015, 1069, 1735, 1780
Tth111I GACNNNGTC 1 cut(s) 407
Vha464I CTTAAG 1 cut(s) 764
VpaK11BI GGWCC 4 cut(s) 393, 578, 777, 1542
XapI RAATTY 1 cut(s) 508
XbaI TCTAGA 1 cut(s) 1393
XspI CTAG 6 cut(s) 438, 1394, 1605, 1811, 1967, 2015
ZraI GACGTC 2 cut(s) 217, 1419
ZrmI AGTACT 2 cut(s) 979, 1965
Zsp2I ATGCAT 1 cut(s) 1764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.