Rh5BG240000

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011080
Physical Location & Seq
Reverse (-)
28763975 .. 28770213
6239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG240000.1

Sequence Viewer

Length: 2079 bp
ATGAGCAAAGCCGGGGAGGAAGAAGACGAAGACGAGTGCTTCTACGAGTCCCTCGATCGCATTCTCGTCTCCTCCTCCTCCTCCTCCTGTTCTTGCTCCACCTCCACCTCCGACGACGACGACGCCGCCGCCGCCGTCTCTTCTTCTCCTCCCAATTATGCTTCCCGCAAGCGCTTTCCCACCCCAAAGTTCCCAATGGGGGCCTACAAGTACGACGTCTGGATCTCCGAGCCCGCCTCCGTCTCCGACCGCCGCTTACGTCTCCTCCGCGAAATGGGCCTCAGCGGCGACCCGGCCCTCTCGCGCGCCAAGCCCGACCCGGTCGGCCGCTCGGCCTCGTCGGATCATTTGACTAAACGACACGGCCCTGCCGCCGTTCCGGAAATCGTCCGGTCCAAATCGGACGGCGTCGAACACTGCAACGGCGCTGCATCCGCTAGTTCGTCGCCTATTCTTTGTTCCGAGATTGAGGCTCCGGCTCCGGCCGAGACCGAGACCAAATATTCGTTTGTAAATACACAGAAAAAAAAAGATTGCGTCGTTTGTAAATCGGCGAGCGGCAAGAGCAATGGACCTTCGCCGTCGCCGCCGAATAGGCCGCCGTCCGGGAAGAACGGCCGGCGGTGCGGCGACGAGATTGGGTTAGAGGTGGAGGAGGAGGAGGAGGAGGAGGAGAGTGAAGTGTGTACCATTCGGAATCTTGATAATGGGAAGGAGTTTGTGGTGAATGAGATCAGGGAAGATGGGATGTGGAACAAGCTTAAGGAAGTGGGGACCGGGAAGCAGTTGACCATGGAGGAGTTTGAGATGAGTGTGGGCCATTCCCCCATTGTTCAGGAACTGATGCGGCGGCAGAATGTGGAGGAGAGCCACAAGGCCGGTGGTGGTTCGGATTCGAATGCCAATGGGGGCGGTGGAGGGATTCCCAAGTTGAAGAAGAGGGGTTGGTTCAAGAGCATAAAGAGTGTTGCGAGTACTGTGACTGGGCATAAGGAGAGAAGGAGCAGTGATGAGAGGGACACTTCGTCGGAGAAGGGGGGCCGGAGGTCAAGCTCTGCCACTGATGATAGCCAGGATGTGACGTTTCATGGGCCGGAGAGAGTGAGGGTCAGGCAGTATGGCAAGTCTTGTAAGGAGCTGACGGCTATGTACAAGACACAGGAGATTCAGGCGCATAATGGGTCTATTTGGAGTATTAAGTTTAGCTTGGATGGGAAGTATCTGGCGAGTGCCGGTGAGGATTGTGTGATTCATATATGGCAGGTGATGGAGACGGAGAGGAAGGGTGACCTGTTGATGCAGAAATCGGAAGATAACAATTTGGATTTGTTGTTTATTGCCAATGGATCTTCAGAACCATGTTCCGTCTCGCCCAATAGTTACAGCCATCTAGAAAAAAAGAAGAGAGGGAGGACGTCTATCAGTCGGAAATCATTGAGTTTGGAACATTATGTGATTCCAGAGACTATGTTTGCACTCTCAGAAAAGCCCATCTGTTCATTCCAAGGTCATGTAGATGATGTGCTTGACCTCTCTTGGTCCAAGTCTCAGCGCCTGCTCTCATCTTCTATGGATAAAACGGTTCACCTCTGGGATTTGCCTAGCAAGTCTTGTTTGCAGAAGTTTTCACACATTGACTATGTAACTTGCATCCAGTTTAATCCCGTTGATGATAACTACTTCATTAGTGGATCACTTGATGCTAAGGTTCGTATATGGAGTATCACTGATCGCCAAGTGGTTGATTGGAATGATATGCATGAGATGGTCACTGCTGCTTGCTACACTCCTGATGGCCAGGGTGCTCTAGTTGGAACCTACAAAGGGAGCTGCCGGTTATACAACACATCTGATAATAAGTTGCAAGAGAAAAGCCAAATAAATCTGCAGAACAAGAAAAAGAAATCTCATCACAAGAAAATTACTGGTTTCCAGTTTGCACCAGGAAGTTCCTCGGAAGTACTAGTTACATCTGCAGATTCACGAGTCAGGGTTGTCGATGGTATTGATCTAGTTCACAAGTTCAAGGGTAAGTATTCATTTTCTATTTTTGTTGTTGTTGTTGTTTTAGCCATTTAA

Protein Analysis

692

Amino Acids

76.3

Weight (kDa)

6.35

Isoelectric Point (pI)

58.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 381 - 424 4.6e-08 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 383 - 427 9.8e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 386 - 420 9.1e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 389 - 429 1.2e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 494 - 575 9.7e-13 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 495 - 621 2.7e-14 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 495 - 558 4.6e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 496 - 677 2e-25 WDR5 beta-propeller domain
EIF3I PF24805 496 - 671 1.6e-07 EIF3I
WD40 PF00400 496 - 532 1.7e-07 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 497 - 664 3.1e-13 CDC20/Fizzy WD40 domain
WDR55 PF24796 498 - 626 5.4e-07 WDR55
Beta-prop_IFT122_1st PF23381 500 - 618 2.4e-07 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 501 - 580 9.5e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 503 - 626 2.2e-09 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 504 - 668 7.4e-18 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 507 - 667 7.5e-10 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 516 - 624 8.2e-09 WDR90/POC16, second beta-propeller
Beta-prop_EML_2 PF23414 558 - 675 4e-09 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15470 AT5G54200
fragaria_vesca FvH4_3g20610 FvH4_3g20610
malus_domestica MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis RchiOBHm_Chr5g0034631
rosa_laevigata RLG00000033560
rosa_roxburghii Rroxscaffold_1G00045690
rosa_rugosa Rorug05G0147300
rosa_samantha Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana Rw5G021930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1252
AatII GACGTC 2 cut(s) 219, 1418
Acc36I ACCTGC 1 cut(s) 1252
AccBSI CCGCTC 2 cut(s) 330, 558
AccII CGCG 3 cut(s) 270, 304, 306
AccIII TCCGGA 1 cut(s) 379
AclWI GGATC 4 cut(s) 230, 351, 1354, 1699
AcoI YGGCCR 4 cut(s) 325, 483, 616, 1795
AcuI CTGAAG 1 cut(s) 1335
AcyI GRCGYC 4 cut(s) 123, 216, 408, 1415
AfaI GTAC 5 cut(s) 212, 688, 976, 1151, 1962
AfeI AGCGCT 1 cut(s) 173
AfiI CCNNNNNNNGG 4 cut(s) 199, 274, 605, 1824
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 3 cut(s) 934, 952, 2026
AhdI GACNNNNNGTC 1 cut(s) 1024
AhlI ACTAGT 1 cut(s) 1963
AjnI CCWGG 3 cut(s) 1071, 1797, 1942
AluBI AGCT 5 cut(s) 760, 1053, 1138, 1206, 1830
AluI AGCT 5 cut(s) 760, 1053, 1138, 1206, 1830
Alw21I GWGCWC 1 cut(s) 1807
AlwI GGATC 4 cut(s) 230, 351, 1354, 1699
AlwNI CAGNNNCTG 2 cut(s) 841, 1555
Aor13HI TCCGGA 1 cut(s) 379
Aor51HI AGCGCT 1 cut(s) 173
ApeKI GCWGC 3 cut(s) 428, 1775, 1830
ArsI GACNNNNNNTTYG 2 cut(s) 487, 519
AspLEI GCGC 6 cut(s) 174, 306, 308, 428, 1174, 1554
AsuC2I CCSGG 5 cut(s) 13, 293, 320, 607, 778
AsuHPI GGTGA 5 cut(s) 736, 1247, 1276, 1298, 1577
AsuII TTCGAA 1 cut(s) 896
AvaII GGWCC 4 cut(s) 393, 572, 774, 1539
BalI TGGCCA 1 cut(s) 1797
BanII GRGCYC 1 cut(s) 234
BauI CACGAG 1 cut(s) 1983
BbsI GAAGAC 2 cut(s) 30, 36
Bbv12I GWGCWC 1 cut(s) 1807
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 3 cut(s) 415, 1762, 1817
BccI CCATC 8 cut(s) 737, 1205, 1261, 1395, 1499, 1759, 1787, 1994
BceAI ACGGC 9 cut(s) 119, 359, 379, 421, 439, 565, 586, 631, 1158
BciT130I CCWGG 3 cut(s) 1073, 1799, 1944
BcnI CCSGG 5 cut(s) 13, 293, 320, 607, 778
BcuI ACTAGT 1 cut(s) 1963
BfaI CTAG 6 cut(s) 438, 1391, 1602, 1808, 1964, 2012
BfmI CTRYAG 2 cut(s) 1886, 1974
BfoI RGCGCY 3 cut(s) 175, 429, 1555
BfrI CTTAAG 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 1252
BglI GCCNNNNNGGC 2 cut(s) 285, 595
BmcAI AGTACT 2 cut(s) 976, 1962
Bme1390I CCNGG 8 cut(s) 13, 293, 320, 607, 778, 1073, 1799, 1944
Bme18I GGWCC 4 cut(s) 393, 572, 774, 1539
BmeRI GACNNNNNGTC 1 cut(s) 1024
BmiI GGNNCC 6 cut(s) 202, 474, 480, 775, 1040, 1816
BmrFI CCNGG 8 cut(s) 13, 293, 320, 607, 778, 1073, 1799, 1944
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 5 cut(s) 440, 834, 1287, 1659, 1690
BmuI ACTGGG 1 cut(s) 993
BpiI GAAGAC 2 cut(s) 30, 36
BplI GAGNNNNNCTC 2 cut(s) 221, 253
Bpu10I CCTNAGC 2 cut(s) 281, 1704
Bpu14I TTCGAA 1 cut(s) 896
BpuMI CCSGG 5 cut(s) 13, 293, 320, 607, 778
BsaHI GRCGYC 4 cut(s) 123, 216, 408, 1415
BsaI GGTCTC 2 cut(s) 482, 488
BsaJI CCNNGG 5 cut(s) 12, 792, 1504, 1798, 1953
BsaWI WCCGGW 2 cut(s) 379, 390
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 274, 605, 1824
Bse118I RCCGGY 4 cut(s) 618, 878, 1232, 1833
Bse1I ACTGG 4 cut(s) 988, 1654, 1930, 1933
Bse3DI GCAATG 1 cut(s) 574
BseAI TCCGGA 1 cut(s) 379
BseBI CCWGG 3 cut(s) 1073, 1799, 1944
BseDI CCNNGG 5 cut(s) 12, 792, 1504, 1798, 1953
BseGI GGATG 5 cut(s) 431, 753, 1081, 1216, 1650
BseLI CCNNNNNNNGG 4 cut(s) 199, 274, 605, 1824
BseMI GCAATG 1 cut(s) 574
BseMII CTCAG 3 cut(s) 295, 1494, 1562
BseNI ACTGG 4 cut(s) 988, 1654, 1930, 1933
BsePI GCGCGC 1 cut(s) 304
BseX3I CGGCCG 3 cut(s) 325, 483, 616
BseXI GCAGC 3 cut(s) 415, 1762, 1817
Bsh1236I CGCG 3 cut(s) 270, 304, 306
Bsh1285I CGRYCG 6 cut(s) 58, 250, 324, 328, 486, 619
BsiEI CGRYCG 6 cut(s) 58, 250, 324, 328, 486, 619
BsiHKAI GWGCWC 1 cut(s) 1807
BslFI GGGAC 3 cut(s) 34, 787, 1031
BslI CCNNNNNNNGG 4 cut(s) 199, 274, 605, 1824
BsmBI CGTCTC 6 cut(s) 73, 142, 247, 266, 1265, 1372
BsmFI GGGAC 3 cut(s) 34, 787, 1031
BsmI GAATGC 2 cut(s) 60, 904
Bso31I GGTCTC 2 cut(s) 482, 488
Bsp119I TTCGAA 1 cut(s) 896
Bsp1286I GDGCHC 2 cut(s) 234, 1807
Bsp13I TCCGGA 1 cut(s) 379
Bsp1407I TGTACA 1 cut(s) 1149
Bsp143I GATC 8 cut(s) 55, 222, 343, 732, 1346, 1691, 1729, 2008
Bsp19I CCATGG 1 cut(s) 792
BspCNI CTCAG 3 cut(s) 294, 1493, 1561
BspEI TCCGGA 1 cut(s) 379
BspFNI CGCG 3 cut(s) 270, 304, 306
BspLI GGNNCC 6 cut(s) 202, 474, 480, 775, 1040, 1816
BspMAI CTGCAG 2 cut(s) 1890, 1978
BspMI ACCTGC 1 cut(s) 1252
BspPI GGATC 4 cut(s) 230, 351, 1354, 1699
BspT104I TTCGAA 1 cut(s) 896
BspTI CTTAAG 1 cut(s) 761
BspTNI GGTCTC 2 cut(s) 482, 488
BsrBI CCGCTC 2 cut(s) 330, 558
BsrDI GCAATG 1 cut(s) 574
BsrFI RCCGGY 4 cut(s) 618, 878, 1232, 1833
BsrGI TGTACA 1 cut(s) 1149
BsrI ACTGG 4 cut(s) 988, 1654, 1930, 1933
BssAI RCCGGY 4 cut(s) 618, 878, 1232, 1833
BssECI CCNNGG 5 cut(s) 12, 792, 1504, 1798, 1953
BssHII GCGCGC 1 cut(s) 304
BssMI GATC 8 cut(s) 55, 222, 343, 732, 1346, 1691, 1729, 2008
BssNI GRCGYC 4 cut(s) 123, 216, 408, 1415
BssSI CACGAG 1 cut(s) 1983
BssT1I CCWWGG 2 cut(s) 792, 1504
Bst2BI CACGAG 1 cut(s) 1983
Bst2UI CCWGG 3 cut(s) 1073, 1799, 1944
Bst4CI ACNGT 2 cut(s) 979, 1582
Bst6I CTCTTC 3 cut(s) 145, 932, 1397
BstACI GRCGYC 4 cut(s) 123, 216, 408, 1415
BstAFI CTTAAG 1 cut(s) 761
BstAUI TGTACA 1 cut(s) 1149
BstBI TTCGAA 1 cut(s) 896
BstC8I GCNNGC 7 cut(s) 170, 234, 306, 556, 620, 1556, 1780
BstDEI CTNAG 4 cut(s) 281, 1480, 1548, 1704
BstDSI CCRYGG 1 cut(s) 792
BstEII GGTNACC 1 cut(s) 1286
BstF5I GGATG 5 cut(s) 431, 753, 1081, 1216, 1650
BstFNI CGCG 3 cut(s) 270, 304, 306
BstH2I RGCGCY 3 cut(s) 175, 429, 1555
BstHHI GCGC 6 cut(s) 174, 306, 308, 428, 1174, 1554
BstKTI GATC 8 cut(s) 58, 225, 346, 735, 1349, 1694, 1732, 2011
BstMBI GATC 8 cut(s) 55, 222, 343, 732, 1346, 1691, 1729, 2008
BstMCI CGRYCG 6 cut(s) 58, 250, 324, 328, 486, 619
BstMWI GCNNNNNNNGC 9 cut(s) 131, 276, 285, 310, 434, 564, 586, 595, 624
BstNI CCWGG 3 cut(s) 1073, 1799, 1944
BstPI GGTNACC 1 cut(s) 1286
BstSCI CCNGG 8 cut(s) 11, 291, 318, 605, 776, 1071, 1797, 1942
BstSFI CTRYAG 2 cut(s) 1886, 1974
BstUI CGCG 3 cut(s) 270, 304, 306
BstV1I GCAGC 3 cut(s) 415, 1762, 1817
BstV2I GAAGAC 2 cut(s) 30, 36
BstX2I RGATCY 2 cut(s) 222, 1346
BstYI RGATCY 2 cut(s) 222, 1346
BstZI CGGCCG 3 cut(s) 325, 483, 616
BtgI CCRYGG 1 cut(s) 792
BtsCI GGATG 5 cut(s) 431, 753, 1081, 1216, 1650
BtsI GCAGTG 3 cut(s) 415, 1012, 1770
BtsIMutI CAGTG 5 cut(s) 415, 1012, 1059, 1725, 1770
BveI ACCTGC 1 cut(s) 1252
Cac8I GCNNGC 7 cut(s) 170, 234, 306, 556, 620, 1556, 1780
CaiI CAGNNNCTG 2 cut(s) 841, 1555
CfoI GCGC 6 cut(s) 174, 306, 308, 428, 1174, 1554
Cfr10I RCCGGY 4 cut(s) 618, 878, 1232, 1833
CseI GACGC 3 cut(s) 131, 397, 526
Csp6I GTAC 5 cut(s) 211, 687, 975, 1150, 1961
CspCI CAANNNNNGTGG 2 cut(s) 862, 897
CviAII CATG 5 cut(s) 793, 1088, 1359, 1511, 1760
CviQI GTAC 5 cut(s) 211, 687, 975, 1150, 1961
DdeI CTNAG 4 cut(s) 281, 1480, 1548, 1704
DpnI GATC 8 cut(s) 57, 224, 345, 734, 1348, 1693, 1731, 2010
DpnII GATC 8 cut(s) 55, 222, 343, 732, 1346, 1691, 1729, 2008
DriI GACNNNNNGTC 1 cut(s) 1024
EaeI YGGCCR 4 cut(s) 325, 483, 616, 1795
EagI CGGCCG 3 cut(s) 325, 483, 616
Eam1104I CTCTTC 3 cut(s) 145, 932, 1397
Eam1105I GACNNNNNGTC 1 cut(s) 1024
EarI CTCTTC 3 cut(s) 145, 932, 1397
EclXI CGGCCG 3 cut(s) 325, 483, 616
Eco130I CCWWGG 2 cut(s) 792, 1504
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 2 cut(s) 482, 488
Eco47I GGWCC 4 cut(s) 393, 572, 774, 1539
Eco47III AGCGCT 1 cut(s) 173
Eco52I CGGCCG 3 cut(s) 325, 483, 616
Eco57I CTGAAG 1 cut(s) 1335
Eco91I GGTNACC 1 cut(s) 1286
EcoO109I RGGNCCY 1 cut(s) 201
EcoO65I GGTNACC 1 cut(s) 1286
EcoRII CCWGG 3 cut(s) 1071, 1797, 1942
EcoT14I CCWWGG 2 cut(s) 792, 1504
EcoT22I ATGCAT 1 cut(s) 1761
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 2 cut(s) 792, 1504
Esp3I CGTCTC 6 cut(s) 73, 142, 247, 266, 1265, 1372
FaeI CATG 5 cut(s) 796, 1091, 1362, 1514, 1763
FalI AAGNNNNNCTT 2 cut(s) 1190, 1222
FaqI GGGAC 3 cut(s) 34, 787, 1031
FatI CATG 5 cut(s) 792, 1087, 1358, 1510, 1759
FauI CCCGC 2 cut(s) 173, 241
FokI GGATG 5 cut(s) 418, 760, 1088, 1223, 1637
FriOI GRGCYC 1 cut(s) 234
FspBI CTAG 6 cut(s) 438, 1391, 1602, 1808, 1964, 2012
GlaI GCGC 6 cut(s) 173, 305, 307, 427, 1173, 1553
HaeII RGCGCY 3 cut(s) 175, 429, 1555
HgaI GACGC 3 cut(s) 131, 397, 526
HhaI GCGC 6 cut(s) 174, 306, 308, 428, 1174, 1554
Hin1I GRCGYC 4 cut(s) 123, 216, 408, 1415
Hin1II CATG 5 cut(s) 796, 1091, 1362, 1514, 1763
Hin6I GCGC 6 cut(s) 172, 304, 306, 426, 1172, 1552
HinP1I GCGC 6 cut(s) 172, 304, 306, 426, 1172, 1552
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 9 cut(s) 47, 697, 893, 922, 1165, 1249, 1456, 1979, 1986
HphI GGTGA 5 cut(s) 736, 1247, 1276, 1298, 1577
Hpy166II GTNNAC 4 cut(s) 687, 789, 1585, 2017
Hpy188III TCNNGA 9 cut(s) 220, 380, 701, 836, 952, 1391, 1460, 1790, 1983
Hpy8I GTNNAC 4 cut(s) 687, 789, 1585, 2017
HpyAV CCTTC 5 cut(s) 585, 706, 993, 1027, 1276
HpyCH4III ACNGT 2 cut(s) 979, 1582
HpyCH4IV ACGT 4 cut(s) 216, 259, 1082, 1415
HpyF10VI GCNNNNNNNGC 9 cut(s) 131, 276, 285, 310, 434, 564, 586, 595, 624
HpyF3I CTNAG 4 cut(s) 281, 1480, 1548, 1704
HpySE526I ACGT 4 cut(s) 216, 259, 1082, 1415
Hsp92I GRCGYC 4 cut(s) 123, 216, 408, 1415
Hsp92II CATG 5 cut(s) 796, 1091, 1362, 1514, 1763
HspAI GCGC 6 cut(s) 172, 304, 306, 426, 1172, 1552
Kpn2I TCCGGA 1 cut(s) 379
KroI GCCGGC 1 cut(s) 618
KroNI GCCGGC 1 cut(s) 620
Kzo9I GATC 8 cut(s) 55, 222, 343, 732, 1346, 1691, 1729, 2008
LmnI GCTCC 6 cut(s) 101, 478, 484, 1002, 1135, 1827
Lsp1109I GCAGC 3 cut(s) 415, 1762, 1817
LweI GCATC 5 cut(s) 440, 834, 1287, 1659, 1690
MaeI CTAG 6 cut(s) 438, 1391, 1602, 1808, 1964, 2012
MaeII ACGT 4 cut(s) 216, 259, 1082, 1415
MaeIII GTNAC 7 cut(s) 979, 1078, 1286, 1379, 1642, 1768, 1966
MalI GATC 8 cut(s) 57, 224, 345, 734, 1348, 1693, 1731, 2010
MbiI CCGCTC 2 cut(s) 330, 558
MboI GATC 8 cut(s) 55, 222, 343, 732, 1346, 1691, 1729, 2008
MflI RGATCY 2 cut(s) 222, 1346
MhlI GDGCHC 2 cut(s) 234, 1807
MlsI TGGCCA 1 cut(s) 1797
MluCI AATT 3 cut(s) 154, 1318, 1920
MluNI TGGCCA 1 cut(s) 1797
MlyI GAGTC 2 cut(s) 56, 1995
MmeI TCCRAC 6 cut(s) 135, 270, 321, 1008, 1406, 1792
Mox20I TGGCCA 1 cut(s) 1797
Mph1103I ATGCAT 1 cut(s) 1761
MroI TCCGGA 1 cut(s) 379
MroNI GCCGGC 1 cut(s) 618
MscI TGGCCA 1 cut(s) 1797
MseI TTAA 4 cut(s) 762, 1197, 1659, 2077
MslI CAYNNNNRTG 1 cut(s) 1515
Msp20I TGGCCA 1 cut(s) 1797
MspA1I CMGCKG 1 cut(s) 285
MspCI CTTAAG 1 cut(s) 761
MspR9I CCNGG 8 cut(s) 13, 293, 320, 607, 778, 1073, 1799, 1944
Mva1269I GAATGC 2 cut(s) 60, 904
MvaI CCWGG 3 cut(s) 1073, 1799, 1944
MvnI CGCG 3 cut(s) 270, 304, 306
MwoI GCNNNNNNNGC 9 cut(s) 131, 276, 285, 310, 434, 564, 586, 595, 624
NaeI GCCGGC 1 cut(s) 620
NciI CCSGG 5 cut(s) 13, 293, 320, 607, 778
NcoI CCATGG 1 cut(s) 792
NdeII GATC 8 cut(s) 55, 222, 343, 732, 1346, 1691, 1729, 2008
NgoMIV GCCGGC 1 cut(s) 618
NlaIII CATG 5 cut(s) 796, 1091, 1362, 1514, 1763
NlaIV GGNNCC 6 cut(s) 202, 474, 480, 775, 1040, 1816
NmeAIII GCCGAG 2 cut(s) 311, 511
NmuCI GTSAC 4 cut(s) 979, 1078, 1286, 1768
NsiI ATGCAT 1 cut(s) 1761
NspV TTCGAA 1 cut(s) 896
PaqCI CACCTGC 1 cut(s) 1252
PauI GCGCGC 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 2 cut(s) 60, 904
PdiI GCCGGC 1 cut(s) 620
PfeI GAWTC 7 cut(s) 697, 893, 922, 1165, 1249, 1456, 1979
PflFI GACNNNGTC 2 cut(s) 320, 407
PfoI TCCNGGA 1 cut(s) 605
Ple19I CGATCG 1 cut(s) 58
PleI GAGTC 2 cut(s) 55, 1994
PpsI GAGTC 2 cut(s) 55, 1994
Psp6I CCWGG 3 cut(s) 1071, 1797, 1942
PspEI GGTNACC 1 cut(s) 1286
PspGI CCWGG 3 cut(s) 1071, 1797, 1942
PspN4I GGNNCC 6 cut(s) 202, 474, 480, 775, 1040, 1816
PstI CTGCAG 2 cut(s) 1890, 1978
PstNI CAGNNNCTG 2 cut(s) 841, 1555
PsuI RGATCY 2 cut(s) 222, 1346
PsyI GACNNNGTC 2 cut(s) 320, 407
PteI GCGCGC 1 cut(s) 304
PvuI CGATCG 1 cut(s) 58
RsaI GTAC 5 cut(s) 212, 688, 976, 1151, 1962
RsaNI GTAC 5 cut(s) 211, 687, 975, 1150, 1961
RseI CAYNNNNRTG 1 cut(s) 1515
SaqAI TTAA 4 cut(s) 762, 1197, 1659, 2077
Sau3AI GATC 8 cut(s) 55, 222, 343, 732, 1346, 1691, 1729, 2008
ScaI AGTACT 2 cut(s) 976, 1962
SchI GAGTC 2 cut(s) 56, 1995
ScrFI CCNGG 8 cut(s) 13, 293, 320, 607, 778, 1073, 1799, 1944
SduI GDGCHC 2 cut(s) 234, 1807
SfaNI GCATC 5 cut(s) 440, 834, 1287, 1659, 1690
SfcI CTRYAG 2 cut(s) 1886, 1974
SfuI TTCGAA 1 cut(s) 896
SinI GGWCC 4 cut(s) 393, 572, 774, 1539
SmiMI CAYNNNNRTG 1 cut(s) 1515
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
SpeI ACTAGT 1 cut(s) 1963
Sse9I AATT 3 cut(s) 154, 1318, 1920
SspI AATATT 1 cut(s) 503
SspMI CTAG 6 cut(s) 438, 1391, 1602, 1808, 1964, 2012
StyD4I CCNGG 8 cut(s) 11, 291, 318, 605, 776, 1071, 1797, 1942
StyI CCWWGG 2 cut(s) 792, 1504
TaaI ACNGT 2 cut(s) 979, 1582
TaiI ACGT 4 cut(s) 219, 262, 1085, 1418
TaqI TCGA 4 cut(s) 54, 411, 896, 1998
TaqII GACCGA 1 cut(s) 506
TasI AATT 3 cut(s) 154, 1318, 1920
TatI WGTACW 3 cut(s) 974, 1149, 1960
TfiI GAWTC 7 cut(s) 697, 893, 922, 1165, 1249, 1456, 1979
Tru1I TTAA 4 cut(s) 762, 1197, 1659, 2077
Tru9I TTAA 4 cut(s) 762, 1197, 1659, 2077
TscAI CASTG 5 cut(s) 422, 1012, 1066, 1732, 1777
TseFI GTSAC 4 cut(s) 979, 1078, 1286, 1768
TseI GCWGC 3 cut(s) 428, 1775, 1830
Tsp45I GTSAC 4 cut(s) 979, 1078, 1286, 1768
TspDTI ATGAA 5 cut(s) 1076, 1241, 1488, 1672, 2028
TspGWI ACGGA 3 cut(s) 229, 1289, 1354
TspRI CASTG 5 cut(s) 422, 1012, 1066, 1732, 1777
Tth111I GACNNNGTC 2 cut(s) 320, 407
Vha464I CTTAAG 1 cut(s) 761
VpaK11BI GGWCC 4 cut(s) 393, 572, 774, 1539
XbaI TCTAGA 1 cut(s) 1390
XcmI CCANNNNNNNNNTGG 1 cut(s) 878
XspI CTAG 6 cut(s) 438, 1391, 1602, 1808, 1964, 2012
ZraI GACGTC 2 cut(s) 217, 1416
ZrmI AGTACT 2 cut(s) 976, 1962
Zsp2I ATGCAT 1 cut(s) 1761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.