RLG00000033560

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011080
Physical Location & Seq
Reverse (-)
28970143 .. 28975388
5246 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033560

Sequence Viewer

Length: 2637 bp
ATGAGCAAAGCCGGGGAGGAAGAAGACGAAGACGAGTGCTTCTACGAGTCCCTCGATCGCATTCTCGTCTCCTCCTCCTCCTCCTGTTCTTGCTCCACCTCCACCTCCGACGACGACGACGACGCCGCCGTCTCTTCTACTCCCAATTATGCTTCCCGCAAGCGCTTTCCCACTCCAAAGTTCCCAATGGGGGCCTACAAGTACGACGTCTGGATCTCCGAGCCCGCCTCCGTCTCCGACCGCCGCTTACGCCTCCTCCGCGAAATGGGCCTCAGCGGCGACCCGGCCCTGTCCCGCGCCAAGCCCGACCCGGTGGGCCGCTCGGCGTCGTCGGATCATTTGACTAAACGACACAGCCCTGCCGCCGTTCCGGAAATCGTCCGGTCCAAATCAGACGGCGTCGAACACTGCAACGGCGCTGCGTCCGCTAGTCCGTCGCCTATTCTTTGTTCCGAGATTGAGGCTCCGGCTCCGGCTCCGGCCGAGACCGAGACCGAGACCAAAAATTCGTTTGTAAATACACAGAAAAAAAAAGATTGCGTTTGTAAATCGGCGAGCGGCAAGAGCAATGGACCTTCTCTGTCGCCGCCGAATAGGCCGCCGTCCGGGAAGAACGGCCGGCGGTGCGGCGACGAGATTCGGTTAGAGGTGGAGGAGCAGCAGCAGGAGGAGGAGGAGGAGAGTGAAGTGTGTACTATTCGGAATCTTGATAATGGGAAGGAGTTTGTGGTGAATGAGATCAGGGAAGATGGGATGTGGAACAAGCTTAAGGAAGTGGGGACCGGGAAGCAGTTGACCATGGAGGAGTTTGAGATGAGTGTGGGGCATTCCCCCATTGTTCAGGAACTGATGCGGCGGCAGAATGTGGAGGAGAGTCACAAGGCCGGCGGCGGTGGTGGTTCGGATTCGAATGCCAATGGGGGCGGCGGAGGGATTCCCAAGTTGAAGAAGAGGGGTTGGTTCAAGAGCATCAAGAGTGTTGCGAGCACTGTGACTGGGCATAAGGAGAGAAGGAGCAGTGATGAGAGGGACACGTCGTCGGAGAAGGGGGGCAGGAGGTCAAGCTCTGCCACTGATGATAGTCAGGATGTGTCGTTTCATGGGCCGGAGAGAGTGAGGGTCAGGCAGTATGCGAAGTCTTGTAAGGATCTAACGGCTGTGTACAAGACACAGGAGATTCAGGCGCATAATGGGTCTATTTGGAGTATTAAGTTTAGCTTGGATGGGAAGTATCTGGCCAGTGCCGGTGAGGATTGTGTGATTCATATATGGCAGGTGATGGAGACCGAGAGGAAGGGTGACCTGTTGATGCAGAAATCAGAAGATAACAATTTGGATTTGTTGTTTATTGCCAATGGATCTTCAGAACCATGTTCCGTCTCGCCCAATAGTTACAGCCATCTGGAAAAAAAGAAGAGAGGGAGGACGTCTATCAGTCGGAAATCATTGAGTTTGGAACATTATGTGATTCCAGAGACTATGTTTGCACTTTCAGAAAAGCCCATCTGTTCATTCCAAGGTCATGTAGATGATGTGCTTGACCTCTCTTGGTCCAAGTCTCAGCACCTGCTCTCATCTTCTATGGATAAAACGGTGCGCCTCTGGGATTTGCCTAACAAGTCTTGTTTGCAGACGTTTTCACACATTGACTATGTAACTTGCATCCAGTTTAATCCCGTTGATGATAACTACTTCATTAGTGGATCACTTGATGCTAAGGTTCGTATATGGAGCATCACTGATCACCAAGTGGTTGATTGGAATGATATGCATGAGATGATCACTGCTGCTTGCTACACTCCTGATGGTCAGGGTGCTCTAGTTGGAACCTACAAAGGGAGCTGCCGGTTATACAACACATCTGATAATAAGTTGCAAGAGAAAAGCCAAATAAATCTGCAGAACAAGAAAAAGAAATCTCATCACAAGAAAATTACTGGTTTCCAGTTTGCACCAGGAAGTTCCTCGGAAGTACTAGTTACATCTGCAGATTCTCGAGTCAGGGTTGTCGATGGTATTGATCTAGTTCACAAGTTCAAGGGATTTCGTAACACAAATAGCCAAATCTCAGCAACCCTCACAGCAAACGGTAAATATGTGGTATCTGCCAGCGAGGATTCTCATGTATATATATGGAAACATGAAGCTGATTCCCGCCCTAGCAGAAGCAAAAGTGTTACTGTTACACGCTCGTATGAGCATTTTCATAGTCACAATGTATCAGTGGCCGTCTCTTGGCCTGGTGTTGGTGATTCATGGGGACTACAAGATGGAGATCAAAATGGACTTGGACTTGACAACAATCTTGATGAGGTTTCTACAGCCAATCATCCCCCTACTCCTGTTGAGGAGGCTAATGGCAAGGATGGTTCACGATCAGCATCGGGTTGCACCAATAGCCCTCTCCATGGAACAATTACTAGTGCATCCAACAGTTACTTCTTTGATAGAATTTCGGCAACGTGGCCTGAGGAAAAACTTCTTCTAGCTACCAAGAAACGAAGCCCTCGTGTAAGTTTTGATTTTAGCAATGGTGTAAGCCAGAACATTCCGGCCTGGGGTATGGTGATTGTAACCGCTGGCTTGGGAGGTGAAATTAGAACTTTCCAAAATTTTGGGTTGCCGATTCGGATTTAA

Protein Analysis

879

Amino Acids

96.51

Weight (kDa)

6.2

Isoelectric Point (pI)

55.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 386 - 428 7.4e-08 THOC3 beta-propeller domain
WD40 PF00400 391 - 424 1.4e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 393 - 433 1.6e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 498 - 579 4.4e-14 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 499 - 625 1.9e-15 Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 499 - 562 1.9e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 500 - 716 3.1e-35 WDR5 beta-propeller domain
WD40 PF00400 500 - 536 8.1e-08 WD domain, G-beta repeat
EIF3I PF24805 501 - 701 5.2e-10 EIF3I
WD40_CDC20-Fz PF24807 501 - 585 4.7e-10 CDC20/Fizzy WD40 domain
WDR55 PF24796 502 - 630 7.8e-07 WDR55
Beta-prop_IFT122_1st PF23381 504 - 621 4.6e-06 IFT122 first beta-propeller
Beta-prop_THOC3 PF25174 504 - 547 5.5e-07 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 505 - 585 3.4e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 507 - 629 2.6e-09 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 508 - 715 5e-23 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 511 - 712 9.4e-13 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 520 - 626 6.8e-09 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 551 - 720 9.8e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 562 - 723 4.9e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 582 - 714 1.1e-07 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15470 AT5G54200
fragaria_vesca FvH4_3g20610 FvH4_3g20610
malus_domestica MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis RchiOBHm_Chr5g0034631
rosa_laevigata RLG00000033560
rosa_roxburghii Rroxscaffold_1G00045690
rosa_rugosa Rorug05G0147300
rosa_samantha Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana Rw5G021930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1264, 1575
AasI GACNNNNNNGTC 1 cut(s) 128
AatII GACGTC 2 cut(s) 210, 1430
Acc36I ACCTGC 2 cut(s) 1264, 1575
AccBSI CCGCTC 2 cut(s) 321, 558
AccII CGCG 2 cut(s) 261, 297
AccIII TCCGGA 1 cut(s) 370
AclWI GGATC 5 cut(s) 221, 342, 1155, 1366, 1711
AcoI YGGCCR 4 cut(s) 480, 616, 1236, 2226
AcsI RAATTY 3 cut(s) 505, 2451, 2611
AcuI CTGAAG 1 cut(s) 1347
AcyI GRCGYC 5 cut(s) 123, 207, 326, 399, 1427
AdeI CACNNNGTG 1 cut(s) 1750
AfaI GTAC 4 cut(s) 203, 694, 1163, 1974
AfeI AGCGCT 1 cut(s) 164
AfiI CCNNNNNNNGG 8 cut(s) 190, 265, 605, 1836, 2235, 2246, 2408, 2558
AflII CTTAAG 1 cut(s) 767
AflIII ACRYGT 1 cut(s) 1032
AgsI TTSAA 3 cut(s) 946, 964, 2038
AhdI GACNNNNNGTC 1 cut(s) 1036
AhlI ACTAGT 2 cut(s) 1975, 2420
AjiI CACGTC 1 cut(s) 1035
AjnI CCWGG 3 cut(s) 1954, 2239, 2555
AjuI GAANNNNNNNTTGG 2 cut(s) 2423, 2455
AluBI AGCT 6 cut(s) 766, 1065, 1218, 1842, 2147, 2489
AluI AGCT 6 cut(s) 766, 1065, 1218, 1842, 2147, 2489
Alw21I GWGCWC 2 cut(s) 989, 1819
AlwI GGATC 5 cut(s) 221, 342, 1155, 1366, 1711
AlwNI CAGNNNCTG 2 cut(s) 847, 1567
Ama87I CYCGRG 1 cut(s) 1995
Aor13HI TCCGGA 1 cut(s) 370
Aor51HI AGCGCT 1 cut(s) 164
ApeKI GCWGC 5 cut(s) 419, 658, 661, 1787, 1842
ApoI RAATTY 3 cut(s) 505, 2451, 2611
ArsI GACNNNNNNTTYG 2 cut(s) 490, 522
Asp700I GAANNNNTTC 1 cut(s) 2478
AspLEI GCGC 5 cut(s) 165, 299, 419, 1186, 1599
AspS9I GGNCC 9 cut(s) 192, 268, 286, 316, 384, 572, 780, 1103, 1551
AsuC2I CCSGG 5 cut(s) 13, 284, 311, 607, 784
AsuHPI GGTGA 8 cut(s) 742, 1259, 1288, 1310, 1736, 2261, 2578, 2603
AsuII TTCGAA 1 cut(s) 908
AvaI CYCGRG 1 cut(s) 1995
AvaII GGWCC 4 cut(s) 384, 572, 780, 1551
AxyI CCTNAGG 1 cut(s) 2469
BalI TGGCCA 1 cut(s) 1238
BanII GRGCYC 1 cut(s) 225
BauI CACGAG 1 cut(s) 2508
BbsI GAAGAC 2 cut(s) 30, 36
Bbv12I GWGCWC 2 cut(s) 989, 1819
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 5 cut(s) 406, 670, 673, 1774, 1829
BccI CCATC 9 cut(s) 743, 1217, 1273, 1407, 1511, 1799, 2006, 2264, 2360
BceAI ACGGC 8 cut(s) 113, 350, 412, 430, 586, 631, 1170, 2213
BciT130I CCWGG 3 cut(s) 1956, 2241, 2557
BclI TGATCA 2 cut(s) 1741, 1779
BcnI CCSGG 5 cut(s) 13, 284, 311, 607, 784
BcuI ACTAGT 2 cut(s) 1975, 2420
BfaI CTAG 7 cut(s) 429, 1820, 1976, 2024, 2160, 2421, 2486
BfmI CTRYAG 3 cut(s) 1898, 1986, 2319
BfoI RGCGCY 2 cut(s) 166, 420
BfrI CTTAAG 1 cut(s) 767
BfuAI ACCTGC 2 cut(s) 1264, 1575
BglI GCCNNNNNGGC 2 cut(s) 276, 595
BmcAI AGTACT 1 cut(s) 1974
Bme1390I CCNGG 8 cut(s) 13, 284, 311, 607, 784, 1956, 2241, 2557
Bme18I GGWCC 4 cut(s) 384, 572, 780, 1551
BmeRI GACNNNNNGTC 1 cut(s) 1036
BmeT110I CYCGRG 1 cut(s) 1995
BmgBI CACGTC 1 cut(s) 1035
BmgT120I GGNCC 9 cut(s) 192, 268, 286, 316, 384, 572, 780, 1103, 1551
BmiI GGNNCC 6 cut(s) 193, 465, 471, 477, 781, 1828
BmrFI CCNGG 8 cut(s) 13, 284, 311, 607, 784, 1956, 2241, 2557
BmrI ACTGGG 1 cut(s) 1005
BmsI GCATC 8 cut(s) 840, 978, 1299, 1671, 1702, 1743, 2390, 2435
BmuI ACTGGG 1 cut(s) 1005
BpiI GAAGAC 2 cut(s) 30, 36
BplI GAGNNNNNCTC 2 cut(s) 212, 244
Bpu10I CCTNAGC 2 cut(s) 272, 1716
Bpu14I TTCGAA 1 cut(s) 908
BpuMI CCSGG 5 cut(s) 13, 284, 311, 607, 784
BsaBI GATNNNNATC 2 cut(s) 2274, 2380
BsaHI GRCGYC 5 cut(s) 123, 207, 326, 399, 1427
BsaI GGTCTC 4 cut(s) 479, 485, 491, 1277
BsaJI CCNNGG 6 cut(s) 12, 798, 1516, 1965, 2407, 2556
BsaWI WCCGGW 2 cut(s) 370, 381
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 8 cut(s) 190, 265, 605, 1836, 2235, 2246, 2408, 2558
Bse118I RCCGGY 4 cut(s) 618, 884, 1244, 1845
Bse1I ACTGG 5 cut(s) 1000, 1239, 1666, 1942, 1945
Bse21I CCTNAGG 1 cut(s) 2469
Bse3DI GCAATG 2 cut(s) 574, 2536
Bse8I GATNNNNATC 2 cut(s) 2274, 2380
BseAI TCCGGA 1 cut(s) 370
BseBI CCWGG 3 cut(s) 1956, 2241, 2557
BseDI CCNNGG 6 cut(s) 12, 798, 1516, 1965, 2407, 2556
BseGI GGATG 7 cut(s) 759, 1093, 1228, 1662, 2329, 2371, 2426
BseJI GATNNNNATC 2 cut(s) 2274, 2380
BseLI CCNNNNNNNGG 8 cut(s) 190, 265, 605, 1836, 2235, 2246, 2408, 2558
BseMI GCAATG 2 cut(s) 574, 2536
BseMII CTCAG 4 cut(s) 286, 1574, 2082, 2460
BseNI ACTGG 5 cut(s) 1000, 1239, 1666, 1942, 1945
BseX3I CGGCCG 2 cut(s) 480, 616
BseXI GCAGC 5 cut(s) 406, 670, 673, 1774, 1829
Bsh1236I CGCG 2 cut(s) 261, 297
Bsh1285I CGRYCG 4 cut(s) 58, 241, 483, 619
BsiEI CGRYCG 4 cut(s) 58, 241, 483, 619
BsiHKAI GWGCWC 2 cut(s) 989, 1819
BsiHKCI CYCGRG 1 cut(s) 1995
BslFI GGGAC 5 cut(s) 34, 277, 793, 1043, 2274
BslI CCNNNNNNNGG 8 cut(s) 190, 265, 605, 1836, 2235, 2246, 2408, 2558
BsmBI CGTCTC 5 cut(s) 73, 136, 238, 1384, 2236
BsmFI GGGAC 5 cut(s) 34, 277, 793, 1043, 2274
BsmI GAATGC 3 cut(s) 60, 826, 916
Bso31I GGTCTC 4 cut(s) 479, 485, 491, 1277
BsoBI CYCGRG 1 cut(s) 1995
Bsp119I TTCGAA 1 cut(s) 908
Bsp1286I GDGCHC 3 cut(s) 225, 989, 1819
Bsp13I TCCGGA 1 cut(s) 370
Bsp1407I TGTACA 1 cut(s) 1161
Bsp19I CCATGG 2 cut(s) 798, 2407
BspCNI CTCAG 4 cut(s) 285, 1573, 2081, 2461
BspEI TCCGGA 1 cut(s) 370
BspFNI CGCG 2 cut(s) 261, 297
BspLI GGNNCC 6 cut(s) 193, 465, 471, 477, 781, 1828
BspMAI CTGCAG 2 cut(s) 1902, 1990
BspMI ACCTGC 2 cut(s) 1264, 1575
BspPI GGATC 5 cut(s) 221, 342, 1155, 1366, 1711
BspT104I TTCGAA 1 cut(s) 908
BspTI CTTAAG 1 cut(s) 767
BspTNI GGTCTC 4 cut(s) 479, 485, 491, 1277
BsrBI CCGCTC 2 cut(s) 321, 558
BsrDI GCAATG 2 cut(s) 574, 2536
BsrFI RCCGGY 4 cut(s) 618, 884, 1244, 1845
BsrGI TGTACA 1 cut(s) 1161
BsrI ACTGG 5 cut(s) 1000, 1239, 1666, 1942, 1945
BssAI RCCGGY 4 cut(s) 618, 884, 1244, 1845
BssECI CCNNGG 6 cut(s) 12, 798, 1516, 1965, 2407, 2556
BssNI GRCGYC 5 cut(s) 123, 207, 326, 399, 1427
BssSI CACGAG 1 cut(s) 2508
BssT1I CCWWGG 3 cut(s) 798, 1516, 2407
Bst2BI CACGAG 1 cut(s) 2508
Bst2UI CCWGG 3 cut(s) 1956, 2241, 2557
Bst4CI ACNGT 5 cut(s) 991, 1594, 2090, 2182, 2435
Bst6I CTCTTC 3 cut(s) 139, 944, 1409
BstACI GRCGYC 5 cut(s) 123, 207, 326, 399, 1427
BstAFI CTTAAG 1 cut(s) 767
BstAUI TGTACA 1 cut(s) 1161
BstBI TTCGAA 1 cut(s) 908
BstC8I GCNNGC 9 cut(s) 161, 225, 556, 620, 886, 985, 1792, 2110, 2581
BstDEI CTNAG 5 cut(s) 272, 1560, 1716, 2068, 2469
BstDSI CCRYGG 2 cut(s) 798, 2407
BstEII GGTNACC 1 cut(s) 1298
BstF5I GGATG 7 cut(s) 759, 1093, 1228, 1662, 2329, 2371, 2426
BstFNI CGCG 2 cut(s) 261, 297
BstH2I RGCGCY 2 cut(s) 166, 420
BstHHI GCGC 5 cut(s) 165, 299, 419, 1186, 1599
BstMCI CGRYCG 4 cut(s) 58, 241, 483, 619
BstMWI GCNNNNNNNGC 9 cut(s) 249, 258, 267, 276, 425, 564, 595, 624, 2397
BstNI CCWGG 3 cut(s) 1956, 2241, 2557
BstPI GGTNACC 1 cut(s) 1298
BstSCI CCNGG 8 cut(s) 11, 282, 309, 605, 782, 1954, 2239, 2555
BstSFI CTRYAG 3 cut(s) 1898, 1986, 2319
BstUI CGCG 2 cut(s) 261, 297
BstV1I GCAGC 5 cut(s) 406, 670, 673, 1774, 1829
BstV2I GAAGAC 2 cut(s) 30, 36
BstX2I RGATCY 3 cut(s) 213, 1147, 1358
BstXI CCANNNNNNTGG 1 cut(s) 2615
BstYI RGATCY 3 cut(s) 213, 1147, 1358
BstZI CGGCCG 2 cut(s) 480, 616
Bsu36I CCTNAGG 1 cut(s) 2469
BtgI CCRYGG 2 cut(s) 798, 2407
BtrI CACGTC 1 cut(s) 1035
BtsCI GGATG 7 cut(s) 759, 1093, 1228, 1662, 2329, 2371, 2426
BtsI GCAGTG 3 cut(s) 406, 1024, 1782
BtsIMutI CAGTG 8 cut(s) 406, 987, 1024, 1071, 1246, 1737, 1782, 2229
BveI ACCTGC 2 cut(s) 1264, 1575
Cac8I GCNNGC 9 cut(s) 161, 225, 556, 620, 886, 985, 1792, 2110, 2581
CaiI CAGNNNCTG 2 cut(s) 847, 1567
CfoI GCGC 5 cut(s) 165, 299, 419, 1186, 1599
Cfr10I RCCGGY 4 cut(s) 618, 884, 1244, 1845
Cfr13I GGNCC 9 cut(s) 192, 268, 286, 316, 384, 572, 780, 1103, 1551
CseI GACGC 4 cut(s) 131, 315, 388, 411
Csp6I GTAC 4 cut(s) 202, 693, 1162, 1973
CviAII CATG 9 cut(s) 799, 1100, 1371, 1523, 1772, 2123, 2141, 2256, 2408
CviQI GTAC 4 cut(s) 202, 693, 1162, 1973
DdeI CTNAG 5 cut(s) 272, 1560, 1716, 2068, 2469
DraIII CACNNNGTG 1 cut(s) 1750
DrdI GACNNNNNNGTC 1 cut(s) 128
DriI GACNNNNNGTC 1 cut(s) 1036
DseDI GACNNNNNNGTC 1 cut(s) 128
EaeI YGGCCR 4 cut(s) 480, 616, 1236, 2226
EagI CGGCCG 2 cut(s) 480, 616
Eam1104I CTCTTC 3 cut(s) 139, 944, 1409
Eam1105I GACNNNNNGTC 1 cut(s) 1036
EarI CTCTTC 3 cut(s) 139, 944, 1409
EciI GGCGGA 1 cut(s) 942
EclXI CGGCCG 2 cut(s) 480, 616
Eco130I CCWWGG 3 cut(s) 798, 1516, 2407
Eco24I GRGCYC 1 cut(s) 225
Eco31I GGTCTC 4 cut(s) 479, 485, 491, 1277
Eco47I GGWCC 4 cut(s) 384, 572, 780, 1551
Eco47III AGCGCT 1 cut(s) 164
Eco52I CGGCCG 2 cut(s) 480, 616
Eco57I CTGAAG 1 cut(s) 1347
Eco81I CCTNAGG 1 cut(s) 2469
Eco88I CYCGRG 1 cut(s) 1995
Eco91I GGTNACC 1 cut(s) 1298
EcoO109I RGGNCCY 1 cut(s) 192
EcoO65I GGTNACC 1 cut(s) 1298
EcoRII CCWGG 3 cut(s) 1954, 2239, 2555
EcoT14I CCWWGG 3 cut(s) 798, 1516, 2407
EcoT22I ATGCAT 1 cut(s) 1773
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 3 cut(s) 798, 1516, 2407
Esp3I CGTCTC 5 cut(s) 73, 136, 238, 1384, 2236
FaeI CATG 9 cut(s) 802, 1103, 1374, 1526, 1775, 2126, 2144, 2259, 2411
FalI AAGNNNNNCTT 2 cut(s) 1202, 1234
FaqI GGGAC 5 cut(s) 34, 277, 793, 1043, 2274
FatI CATG 9 cut(s) 798, 1099, 1370, 1522, 1771, 2122, 2140, 2255, 2407
FauI CCCGC 4 cut(s) 164, 232, 302, 2162
FbaI TGATCA 2 cut(s) 1741, 1779
FokI GGATG 7 cut(s) 766, 1100, 1235, 1649, 2316, 2378, 2413
FriOI GRGCYC 1 cut(s) 225
FspBI CTAG 7 cut(s) 429, 1820, 1976, 2024, 2160, 2421, 2486
GlaI GCGC 5 cut(s) 164, 298, 418, 1185, 1598
HaeII RGCGCY 2 cut(s) 166, 420
HgaI GACGC 4 cut(s) 131, 315, 388, 411
HhaI GCGC 5 cut(s) 165, 299, 419, 1186, 1599
Hin1I GRCGYC 5 cut(s) 123, 207, 326, 399, 1427
Hin1II CATG 9 cut(s) 802, 1103, 1374, 1526, 1775, 2126, 2144, 2259, 2411
Hin6I GCGC 5 cut(s) 163, 297, 417, 1184, 1597
HinP1I GCGC 5 cut(s) 163, 297, 417, 1184, 1597
HincII GTYRAC 1 cut(s) 795
HindII GTYRAC 1 cut(s) 795
HindIII AAGCTT 1 cut(s) 764
HphI GGTGA 8 cut(s) 742, 1259, 1288, 1310, 1736, 2261, 2578, 2603
Hpy166II GTNNAC 5 cut(s) 693, 795, 1162, 2029, 2372
Hpy8I GTNNAC 5 cut(s) 693, 795, 1162, 2029, 2372
HpyAV CCTTC 5 cut(s) 585, 712, 1005, 1039, 1288
HpyCH4III ACNGT 5 cut(s) 991, 1594, 2090, 2182, 2435
HpyCH4IV ACGT 5 cut(s) 207, 1034, 1427, 1634, 2462
HpyF10VI GCNNNNNNNGC 9 cut(s) 249, 258, 267, 276, 425, 564, 595, 624, 2397
HpyF3I CTNAG 5 cut(s) 272, 1560, 1716, 2068, 2469
HpySE526I ACGT 5 cut(s) 207, 1034, 1427, 1634, 2462
Hsp92I GRCGYC 5 cut(s) 123, 207, 326, 399, 1427
Hsp92II CATG 9 cut(s) 802, 1103, 1374, 1526, 1775, 2126, 2144, 2259, 2411
HspAI GCGC 5 cut(s) 163, 297, 417, 1184, 1597
Kpn2I TCCGGA 1 cut(s) 370
KroI GCCGGC 2 cut(s) 618, 884
KroNI GCCGGC 2 cut(s) 620, 886
Ksp22I TGATCA 2 cut(s) 1741, 1779
LmnI GCTCC 8 cut(s) 98, 469, 475, 481, 655, 1014, 1731, 1839
Lsp1109I GCAGC 5 cut(s) 406, 670, 673, 1774, 1829
LweI GCATC 8 cut(s) 840, 978, 1299, 1671, 1702, 1743, 2390, 2435
MaeI CTAG 7 cut(s) 429, 1820, 1976, 2024, 2160, 2421, 2486
MaeII ACGT 5 cut(s) 207, 1034, 1427, 1634, 2462
MbiI CCGCTC 2 cut(s) 321, 558
MflI RGATCY 3 cut(s) 213, 1147, 1358
MhlI GDGCHC 3 cut(s) 225, 989, 1819
MlsI TGGCCA 1 cut(s) 1238
MluCI AATT 8 cut(s) 145, 505, 1330, 1932, 2415, 2451, 2595, 2611
MluNI TGGCCA 1 cut(s) 1238
MlyI GAGTC 3 cut(s) 56, 883, 2007
MmeI TCCRAC 7 cut(s) 132, 261, 312, 1020, 1418, 1804, 2454
Mox20I TGGCCA 1 cut(s) 1238
Mph1103I ATGCAT 1 cut(s) 1773
MroI TCCGGA 1 cut(s) 370
MroNI GCCGGC 2 cut(s) 618, 884
MroXI GAANNNNTTC 1 cut(s) 2478
MscI TGGCCA 1 cut(s) 1238
MseI TTAA 4 cut(s) 768, 1209, 1671, 2635
MslI CAYNNNNRTG 1 cut(s) 1527
Msp20I TGGCCA 1 cut(s) 1238
MspA1I CMGCKG 2 cut(s) 276, 2579
MspCI CTTAAG 1 cut(s) 767
MspR9I CCNGG 8 cut(s) 13, 284, 311, 607, 784, 1956, 2241, 2557
Mva1269I GAATGC 3 cut(s) 60, 826, 916
MvaI CCWGG 3 cut(s) 1956, 2241, 2557
MvnI CGCG 2 cut(s) 261, 297
MwoI GCNNNNNNNGC 9 cut(s) 249, 258, 267, 276, 425, 564, 595, 624, 2397
NaeI GCCGGC 2 cut(s) 620, 886
NciI CCSGG 5 cut(s) 13, 284, 311, 607, 784
NcoI CCATGG 2 cut(s) 798, 2407
NgoMIV GCCGGC 2 cut(s) 618, 884
NlaIII CATG 9 cut(s) 802, 1103, 1374, 1526, 1775, 2126, 2144, 2259, 2411
NlaIV GGNNCC 6 cut(s) 193, 465, 471, 477, 781, 1828
NmeAIII GCCGAG 2 cut(s) 302, 508
NmuCI GTSAC 4 cut(s) 875, 991, 1298, 2210
NsiI ATGCAT 1 cut(s) 1773
NspV TTCGAA 1 cut(s) 908
PaeR7I CTCGAG 1 cut(s) 1995
PaqCI CACCTGC 2 cut(s) 1264, 1575
PcsI WCGNNNNNNNCGW 4 cut(s) 51, 117, 126, 2506
PctI GAATGC 3 cut(s) 60, 826, 916
PdiI GCCGGC 2 cut(s) 620, 886
PdmI GAANNNNTTC 1 cut(s) 2478
PflFI GACNNNGTC 1 cut(s) 398
PfoI TCCNGGA 1 cut(s) 605
Ple19I CGATCG 1 cut(s) 58
PleI GAGTC 3 cut(s) 55, 882, 2006
PpsI GAGTC 3 cut(s) 55, 882, 2006
Psp6I CCWGG 3 cut(s) 1954, 2239, 2555
PspEI GGTNACC 1 cut(s) 1298
PspGI CCWGG 3 cut(s) 1954, 2239, 2555
PspN4I GGNNCC 6 cut(s) 193, 465, 471, 477, 781, 1828
PspPI GGNCC 9 cut(s) 192, 268, 286, 316, 384, 572, 780, 1103, 1551
PstI CTGCAG 2 cut(s) 1902, 1990
PstNI CAGNNNCTG 2 cut(s) 847, 1567
PsuI RGATCY 3 cut(s) 213, 1147, 1358
PsyI GACNNNGTC 1 cut(s) 398
PvuI CGATCG 1 cut(s) 58
RsaI GTAC 4 cut(s) 203, 694, 1163, 1974
RsaNI GTAC 4 cut(s) 202, 693, 1162, 1973
RseI CAYNNNNRTG 1 cut(s) 1527
SaqAI TTAA 4 cut(s) 768, 1209, 1671, 2635
Sau96I GGNCC 9 cut(s) 192, 268, 286, 316, 384, 572, 780, 1103, 1551
ScaI AGTACT 1 cut(s) 1974
SchI GAGTC 3 cut(s) 56, 883, 2007
ScrFI CCNGG 8 cut(s) 13, 284, 311, 607, 784, 1956, 2241, 2557
SduI GDGCHC 3 cut(s) 225, 989, 1819
SfaNI GCATC 8 cut(s) 840, 978, 1299, 1671, 1702, 1743, 2390, 2435
SfcI CTRYAG 3 cut(s) 1898, 1986, 2319
Sfr274I CTCGAG 1 cut(s) 1995
SfuI TTCGAA 1 cut(s) 908
SinI GGWCC 4 cut(s) 384, 572, 780, 1551
SlaI CTCGAG 1 cut(s) 1995
SmiMI CAYNNNNRTG 1 cut(s) 1527
SmlI CTYRAG 2 cut(s) 767, 1995
SmoI CTYRAG 2 cut(s) 767, 1995
SpeI ACTAGT 2 cut(s) 1975, 2420
Sse9I AATT 8 cut(s) 145, 505, 1330, 1932, 2415, 2451, 2595, 2611
SspMI CTAG 7 cut(s) 429, 1820, 1976, 2024, 2160, 2421, 2486
StyD4I CCNGG 8 cut(s) 11, 282, 309, 605, 782, 1954, 2239, 2555
StyI CCWWGG 3 cut(s) 798, 1516, 2407
TaaI ACNGT 5 cut(s) 991, 1594, 2090, 2182, 2435
TaiI ACGT 5 cut(s) 210, 1037, 1430, 1637, 2465
TaqI TCGA 5 cut(s) 54, 402, 908, 1996, 2010
TaqII GACCGA 3 cut(s) 503, 509, 1301
TasI AATT 8 cut(s) 145, 505, 1330, 1932, 2415, 2451, 2595, 2611
TatI WGTACW 3 cut(s) 692, 1161, 1972
Tru1I TTAA 4 cut(s) 768, 1209, 1671, 2635
Tru9I TTAA 4 cut(s) 768, 1209, 1671, 2635
TscAI CASTG 8 cut(s) 413, 994, 1024, 1078, 1246, 1744, 1789, 2229
TseFI GTSAC 4 cut(s) 875, 991, 1298, 2210
TseI GCWGC 5 cut(s) 419, 658, 661, 1787, 1842
Tsp45I GTSAC 4 cut(s) 875, 991, 1298, 2210
TspDTI ATGAA 7 cut(s) 1088, 1253, 1500, 1684, 2157, 2195, 2244
TspGWI ACGGA 3 cut(s) 220, 423, 1366
TspRI CASTG 8 cut(s) 413, 994, 1024, 1078, 1246, 1744, 1789, 2229
Tth111I GACNNNGTC 1 cut(s) 398
Vha464I CTTAAG 1 cut(s) 767
VpaK11BI GGWCC 4 cut(s) 384, 572, 780, 1551
XapI RAATTY 3 cut(s) 505, 2451, 2611
XhoI CTCGAG 1 cut(s) 1995
XmnI GAANNNNTTC 1 cut(s) 2478
XspI CTAG 7 cut(s) 429, 1820, 1976, 2024, 2160, 2421, 2486
ZraI GACGTC 2 cut(s) 208, 1428
ZrmI AGTACT 1 cut(s) 1974
Zsp2I ATGCAT 1 cut(s) 1773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.