Rh5CG270400

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011080
Physical Location & Seq
Reverse (-)
29052515 .. 29057741
5227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG270400.1

Sequence Viewer

Length: 2634 bp
ATGAGCAAAGCCGGGGAGGAAGAAGACGAAAACGAGTGCTTCTACGAGTCCCTCGATCGCATTCTCGTCTCCTCCTCCTCCTCCTGTTCTTGCTCCACCTCCACCTCCGACGACGACGACGACCACGACGCCGCCGTCTCTTCTTCTCCTCCCAATTATGCTTCCCGCAAGCGCTTTCCCACCCCAAAGTTCCCAATGGGGGCCTACAAGTACGACGTCTGGATCTCCGAGCCCGCCTCCGTCTCCGACCGCCGCTTACGTCTCCTCCGCGAAATGGGCCTCAGCGGCGACCCGGCCCTCTCGCGCGCCAAGCTCGACCCGGTGGGCCGCTCGGCCTCGTCGGATCATTTGACTAAACGACACAGCCCTGCCGCCGTTCCGGAAATCGTCCGGTCCAAATCGGACGGCGTCGAACACTGCAACGGCGCTGCATCCGCTAGTTCGTCGCCTATTCTTTGTTCCGAGATTGAGGCTCCGGCTCCGGCTCCGGCCGAGACTGAGACAAAAAATTCGTTTGTAAATACACAGAAAAAAAAAGATTGCGTCGCTTGTAAATCGGCGAGCGGCAAGAGCAATGGACCTTCTCCGTCGCCGCCGAATAGGCCGCCGTCCGGGAAGAACGGCCGGCGGTGCGGCGACGAGACTGGGTTAGAGGTGGAGGAGCAGGAGGAGGAGGAGGAGGAGAGTGAAGTGTGTACTATTCGGAATCTTGATAATGGGAAGGAGTTTGTGGTGAATGAGATCAGGGAAGATGGGATGTGGAACAAGCTTAAGGAAGTGGGGACCGGGAAGCAGTTGACCATGGAGGAGTTTGAGATGAGTGTGGGCCATTCCCCCATTGTTCAGGAACTGATGCGGCGGCAGAATGTGGAGGAGAGTCACAAGGCCGGTGGTGGTTCGGATTCGAATGCCAATGGGGGCGGTGGAGGGATTCCCAAGTTGAAGAAGAGGGGTTGGTTCAAGAGCATAAAGAGTGTTGCGAGTACTGTGACTGGGCATAAGGAGAGAAGGAGCAGTGATGAGAGGGACACTTCGTCGGAGAAGGGGGGCCGGAGGTCAAGCTCTGCCACTGATGATAGCCAGGATGTGTCGTTTCATGGGCCGGAGAGAGTGAGGGTCAGGCAGTATGGCAATTCTTGTAAGGAGCTGACGGCTATGTACAAGACACAGGAGATTCAGGCGCATAATGGGTCTATTTGGAGTATTAAGTTTAGCTTGGATGGGAAGTATCTGGCGAGTGCCGGTGAGGATTGTGTGATTCATATATGGCAGGTGATGGAGACGGAGAGGAAGGGTGACCTGTTGATGCAGAAATCGGAAGATAACAATTTGGATTTGTTGTTTATTGCCAATGGATCTTCAGAACCATGTTCCGTCTCGCCCAATAGTTACAGCCATCTAGAAAAAAAGAAGAGAGGGAGGACGTCTATCAGTCGGAAATCATTGAGTTTGGAACATTATGTGATTCCAGAGACTATGTTTGCACTCTCAGAAAAGCCCATCTGTTCATTCCAAGGTCATGTAGATGATGTGCTTGACCTCTCTTGGTCCAAGTCTCAGCGCCTGCTCTCATCTTCTATGGATAAAACAGTTCACCTCTGGGATTTGCCTAGCAAGTCTTGTTTGCAGAAGTTTTCACACATTGACTATGTAACTTGCATCCAGTTTAATCCCGTTGATGATAACTATTTCATTAGTGGATCACTTGATGCTAAGGTTCGTATATGGAGCATCACTGATCACCAAGTGGTTGATTGGAATGATATGCATGAGATGGTCACTGCTGCTTGCTACACTCCTGATGGCCAGGGTGCTCTAGTTGGAACCTACAAAGGGAGCTGCCGGTTATACAACACATCTGATAATAAGTTGCAAGAGAAAAGCCAAATAAATCTGCAGAACAAGAAAAAGAAATCTCATCACAAGAAAATTACTGGTTTCCAGTTTGCACCAGGAAGTTCCTCGGAAGTACTAGTTACATCTGCAGATTCACGAGTCAGGGTTGTCGATGGTATTGATCTAGTTCACAAGTTCAAGGGATTTCGTAACACAAATAGCCAAATCTCAGCAACCCTCACAGCAAACGGTAAATATGTGGTATCTGCCAGCGAGGATTCTCATGTATATATATGGAAACATGAAGCTGATTCGCGCCCTAGCAGAAGCAAAAGTGTTACTGTTACACGGTCATATGAGCATTTTCATAGTCACAATGTATCAGTGGCCGTCTCTTGGCCTGGTGTTGGTGATTCATGGGGACTACAAGATGGAGATCAAAATGGACTTGGACTAGACAACAATCTTGATGAGGTTTCTACAGCCAATCATCCCCCTACTCCTGTTGAGGAGGCTAATGGCAAGGATGGTTCACGATCAGCATCGGGTTGCACCAATAGCCCTCTCCATGGAACAATTACTAGTGCATCCAACAGTTACTTCTTTGATAGAATTTCGGCAACGTGGCCTGAGGAAAAACTTCTTCTAGCTACTAAGAAACGAAGCCCTCGTGTAAGTTTTGATTTTAGCAATGGTGTAAGCCAGAACATTCCGGCCTGGGGTATGGTGATTGTAACCGCTGGCTTGGGAGGTGAAATTAGAACTTTCCAAAATTTTGGGTTGCCGATTCGGATTTAA

Protein Analysis

877

Amino Acids

96.33

Weight (kDa)

6.17

Isoelectric Point (pI)

55.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 384 - 427 6.2e-08 THOC3 beta-propeller domain
WD40 PF00400 389 - 423 1.2e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 392 - 432 1.6e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 497 - 578 1.3e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 498 - 624 3.6e-14 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 499 - 715 2.8e-34 WDR5 beta-propeller domain
WD40 PF00400 499 - 535 2.2e-07 WD domain, G-beta repeat
EIF3I PF24805 499 - 700 1.8e-09 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 499 - 561 7.1e-08 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 500 - 594 1.2e-10 CDC20/Fizzy WD40 domain
WDR55 PF24796 501 - 629 9.6e-07 WDR55
Beta-prop_IFT122_1st PF23381 504 - 620 5.2e-07 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 504 - 583 1.3e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 506 - 629 1.7e-09 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 507 - 713 1.5e-22 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 510 - 711 9.5e-12 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 519 - 625 3.2e-08 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 522 - 711 2.1e-08 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 550 - 719 8.9e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 561 - 722 4.2e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 581 - 713 4.6e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15470 AT5G54200
fragaria_vesca FvH4_3g20610 FvH4_3g20610
malus_domestica MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis RchiOBHm_Chr5g0034631
rosa_laevigata RLG00000033560
rosa_roxburghii Rroxscaffold_1G00045690
rosa_rugosa Rorug05G0147300
rosa_samantha Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana Rw5G021930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1261
AasI GACNNNNNNGTC 1 cut(s) 134
AatII GACGTC 2 cut(s) 219, 1427
Acc36I ACCTGC 1 cut(s) 1261
AccBSI CCGCTC 2 cut(s) 330, 564
AccII CGCG 4 cut(s) 270, 304, 306, 2152
AccIII TCCGGA 1 cut(s) 379
AclWI GGATC 4 cut(s) 230, 351, 1363, 1708
AcoI YGGCCR 4 cut(s) 489, 622, 1804, 2223
AcsI RAATTY 3 cut(s) 508, 2448, 2608
AcuI CTGAAG 1 cut(s) 1344
AcyI GRCGYC 4 cut(s) 129, 216, 408, 1424
AdeI CACNNNGTG 1 cut(s) 1747
AfaI GTAC 5 cut(s) 212, 697, 985, 1160, 1971
AfeI AGCGCT 1 cut(s) 173
AfiI CCNNNNNNNGG 8 cut(s) 199, 274, 611, 1833, 2232, 2243, 2405, 2555
AflII CTTAAG 1 cut(s) 770
AgsI TTSAA 3 cut(s) 943, 961, 2035
AhdI GACNNNNNGTC 1 cut(s) 1033
AhlI ACTAGT 2 cut(s) 1972, 2417
AjnI CCWGG 5 cut(s) 1080, 1806, 1951, 2236, 2552
AjuI GAANNNNNNNTTGG 2 cut(s) 2420, 2452
AluBI AGCT 8 cut(s) 313, 769, 1062, 1147, 1215, 1839, 2144, 2486
AluI AGCT 8 cut(s) 313, 769, 1062, 1147, 1215, 1839, 2144, 2486
Alw21I GWGCWC 1 cut(s) 1816
AlwI GGATC 4 cut(s) 230, 351, 1363, 1708
AlwNI CAGNNNCTG 2 cut(s) 850, 1564
Aor13HI TCCGGA 1 cut(s) 379
Aor51HI AGCGCT 1 cut(s) 173
ApeKI GCWGC 3 cut(s) 428, 1784, 1839
ApoI RAATTY 3 cut(s) 508, 2448, 2608
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
Asp700I GAANNNNTTC 1 cut(s) 2475
AspLEI GCGC 7 cut(s) 174, 306, 308, 428, 1183, 1563, 2154
AsuC2I CCSGG 5 cut(s) 13, 293, 320, 613, 787
AsuHPI GGTGA 9 cut(s) 745, 1256, 1285, 1307, 1586, 1733, 2258, 2575, 2600
AsuII TTCGAA 1 cut(s) 905
AvaII GGWCC 4 cut(s) 393, 578, 783, 1548
AxyI CCTNAGG 1 cut(s) 2466
BalI TGGCCA 1 cut(s) 1806
BanII GRGCYC 1 cut(s) 234
BauI CACGAG 2 cut(s) 1992, 2505
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 1816
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 3 cut(s) 415, 1771, 1826
BceAI ACGGC 8 cut(s) 119, 359, 421, 439, 592, 637, 1167, 2210
BciT130I CCWGG 5 cut(s) 1082, 1808, 1953, 2238, 2554
BclI TGATCA 1 cut(s) 1738
BcnI CCSGG 5 cut(s) 13, 293, 320, 613, 787
BcuI ACTAGT 2 cut(s) 1972, 2417
BfmI CTRYAG 3 cut(s) 1895, 1983, 2316
BfoI RGCGCY 3 cut(s) 175, 429, 1564
BfrI CTTAAG 1 cut(s) 770
BfuAI ACCTGC 1 cut(s) 1261
BglI GCCNNNNNGGC 2 cut(s) 285, 601
BmcAI AGTACT 2 cut(s) 985, 1971
Bme18I GGWCC 4 cut(s) 393, 578, 783, 1548
BmeRI GACNNNNNGTC 1 cut(s) 1033
BmiI GGNNCC 7 cut(s) 202, 474, 480, 486, 784, 1049, 1825
BmrI ACTGGG 2 cut(s) 654, 1002
BmsI GCATC 8 cut(s) 440, 843, 1296, 1668, 1699, 1740, 2387, 2432
BmuI ACTGGG 2 cut(s) 654, 1002
BpiI GAAGAC 1 cut(s) 30
BplI GAGNNNNNCTC 2 cut(s) 221, 253
Bpu10I CCTNAGC 2 cut(s) 281, 1713
Bpu14I TTCGAA 1 cut(s) 905
BpuMI CCSGG 5 cut(s) 13, 293, 320, 613, 787
BsaBI GATNNNNATC 2 cut(s) 2271, 2377
BsaHI GRCGYC 4 cut(s) 129, 216, 408, 1424
BsaJI CCNNGG 7 cut(s) 12, 801, 1513, 1807, 1962, 2404, 2553
BsaWI WCCGGW 2 cut(s) 379, 390
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 8 cut(s) 199, 274, 611, 1833, 2232, 2243, 2405, 2555
Bse118I RCCGGY 4 cut(s) 624, 887, 1241, 1842
Bse1I ACTGG 5 cut(s) 649, 997, 1663, 1939, 1942
Bse21I CCTNAGG 1 cut(s) 2466
Bse3DI GCAATG 2 cut(s) 580, 2533
Bse8I GATNNNNATC 2 cut(s) 2271, 2377
BseAI TCCGGA 1 cut(s) 379
BseBI CCWGG 5 cut(s) 1082, 1808, 1953, 2238, 2554
BseDI CCNNGG 7 cut(s) 12, 801, 1513, 1807, 1962, 2404, 2553
BseGI GGATG 8 cut(s) 431, 762, 1090, 1225, 1659, 2326, 2368, 2423
BseJI GATNNNNATC 2 cut(s) 2271, 2377
BseLI CCNNNNNNNGG 8 cut(s) 199, 274, 611, 1833, 2232, 2243, 2405, 2555
BseMI GCAATG 2 cut(s) 580, 2533
BseMII CTCAG 6 cut(s) 295, 489, 1503, 1571, 2079, 2457
BseNI ACTGG 5 cut(s) 649, 997, 1663, 1939, 1942
BsePI GCGCGC 1 cut(s) 304
BseX3I CGGCCG 2 cut(s) 489, 622
BseXI GCAGC 3 cut(s) 415, 1771, 1826
Bsh1236I CGCG 4 cut(s) 270, 304, 306, 2152
Bsh1285I CGRYCG 4 cut(s) 58, 250, 492, 625
BsiEI CGRYCG 4 cut(s) 58, 250, 492, 625
BsiHKAI GWGCWC 1 cut(s) 1816
BslFI GGGAC 4 cut(s) 34, 796, 1040, 2271
BslI CCNNNNNNNGG 8 cut(s) 199, 274, 611, 1833, 2232, 2243, 2405, 2555
BsmBI CGTCTC 7 cut(s) 73, 142, 247, 266, 1274, 1381, 2233
BsmFI GGGAC 4 cut(s) 34, 796, 1040, 2271
BsmI GAATGC 2 cut(s) 60, 913
Bsp119I TTCGAA 1 cut(s) 905
Bsp1286I GDGCHC 2 cut(s) 234, 1816
Bsp13I TCCGGA 1 cut(s) 379
Bsp1407I TGTACA 1 cut(s) 1158
Bsp19I CCATGG 2 cut(s) 801, 2404
BspCNI CTCAG 6 cut(s) 294, 490, 1502, 1570, 2078, 2458
BspEI TCCGGA 1 cut(s) 379
BspFNI CGCG 4 cut(s) 270, 304, 306, 2152
BspLI GGNNCC 7 cut(s) 202, 474, 480, 486, 784, 1049, 1825
BspMAI CTGCAG 2 cut(s) 1899, 1987
BspMI ACCTGC 1 cut(s) 1261
BspPI GGATC 4 cut(s) 230, 351, 1363, 1708
BspT104I TTCGAA 1 cut(s) 905
BspTI CTTAAG 1 cut(s) 770
BsrBI CCGCTC 2 cut(s) 330, 564
BsrDI GCAATG 2 cut(s) 580, 2533
BsrFI RCCGGY 4 cut(s) 624, 887, 1241, 1842
BsrGI TGTACA 1 cut(s) 1158
BsrI ACTGG 5 cut(s) 649, 997, 1663, 1939, 1942
BssAI RCCGGY 4 cut(s) 624, 887, 1241, 1842
BssECI CCNNGG 7 cut(s) 12, 801, 1513, 1807, 1962, 2404, 2553
BssHII GCGCGC 1 cut(s) 304
BssNI GRCGYC 4 cut(s) 129, 216, 408, 1424
BssSI CACGAG 2 cut(s) 1992, 2505
BssT1I CCWWGG 3 cut(s) 801, 1513, 2404
Bst2BI CACGAG 2 cut(s) 1992, 2505
Bst2UI CCWGG 5 cut(s) 1082, 1808, 1953, 2238, 2554
Bst4CI ACNGT 6 cut(s) 988, 1591, 2087, 2179, 2187, 2432
Bst6I CTCTTC 3 cut(s) 145, 941, 1406
BstACI GRCGYC 4 cut(s) 129, 216, 408, 1424
BstAFI CTTAAG 1 cut(s) 770
BstAUI TGTACA 1 cut(s) 1158
BstBI TTCGAA 1 cut(s) 905
BstC8I GCNNGC 9 cut(s) 170, 234, 306, 562, 626, 1565, 1789, 2107, 2578
BstDEI CTNAG 8 cut(s) 281, 498, 1489, 1557, 1713, 2065, 2466, 2490
BstDSI CCRYGG 2 cut(s) 801, 2404
BstEII GGTNACC 1 cut(s) 1295
BstF5I GGATG 8 cut(s) 431, 762, 1090, 1225, 1659, 2326, 2368, 2423
BstFNI CGCG 4 cut(s) 270, 304, 306, 2152
BstH2I RGCGCY 3 cut(s) 175, 429, 1564
BstHHI GCGC 7 cut(s) 174, 306, 308, 428, 1183, 1563, 2154
BstMCI CGRYCG 4 cut(s) 58, 250, 492, 625
BstMWI GCNNNNNNNGC 8 cut(s) 276, 285, 310, 434, 570, 601, 630, 2394
BstNI CCWGG 5 cut(s) 1082, 1808, 1953, 2238, 2554
BstPI GGTNACC 1 cut(s) 1295
BstSFI CTRYAG 3 cut(s) 1895, 1983, 2316
BstUI CGCG 4 cut(s) 270, 304, 306, 2152
BstV1I GCAGC 3 cut(s) 415, 1771, 1826
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 2 cut(s) 222, 1355
BstXI CCANNNNNNTGG 1 cut(s) 2612
BstYI RGATCY 2 cut(s) 222, 1355
BstZI CGGCCG 2 cut(s) 489, 622
Bsu36I CCTNAGG 1 cut(s) 2466
BtgI CCRYGG 2 cut(s) 801, 2404
BtsCI GGATG 8 cut(s) 431, 762, 1090, 1225, 1659, 2326, 2368, 2423
BtsI GCAGTG 3 cut(s) 415, 1021, 1779
BtsIMutI CAGTG 6 cut(s) 415, 1021, 1068, 1734, 1779, 2226
BveI ACCTGC 1 cut(s) 1261
Cac8I GCNNGC 9 cut(s) 170, 234, 306, 562, 626, 1565, 1789, 2107, 2578
CaiI CAGNNNCTG 2 cut(s) 850, 1564
CfoI GCGC 7 cut(s) 174, 306, 308, 428, 1183, 1563, 2154
Cfr10I RCCGGY 4 cut(s) 624, 887, 1241, 1842
CseI GACGC 3 cut(s) 137, 397, 532
Csp6I GTAC 5 cut(s) 211, 696, 984, 1159, 1970
CspCI CAANNNNNGTGG 2 cut(s) 871, 906
CviAII CATG 9 cut(s) 802, 1097, 1368, 1520, 1769, 2120, 2138, 2253, 2405
CviQI GTAC 5 cut(s) 211, 696, 984, 1159, 1970
DdeI CTNAG 8 cut(s) 281, 498, 1489, 1557, 1713, 2065, 2466, 2490
DraIII CACNNNGTG 1 cut(s) 1747
DrdI GACNNNNNNGTC 1 cut(s) 134
DriI GACNNNNNGTC 1 cut(s) 1033
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 4 cut(s) 489, 622, 1804, 2223
EagI CGGCCG 2 cut(s) 489, 622
Eam1104I CTCTTC 3 cut(s) 145, 941, 1406
Eam1105I GACNNNNNGTC 1 cut(s) 1033
EarI CTCTTC 3 cut(s) 145, 941, 1406
EclXI CGGCCG 2 cut(s) 489, 622
Eco130I CCWWGG 3 cut(s) 801, 1513, 2404
Eco24I GRGCYC 1 cut(s) 234
Eco47I GGWCC 4 cut(s) 393, 578, 783, 1548
Eco47III AGCGCT 1 cut(s) 173
Eco52I CGGCCG 2 cut(s) 489, 622
Eco57I CTGAAG 1 cut(s) 1344
Eco81I CCTNAGG 1 cut(s) 2466
Eco91I GGTNACC 1 cut(s) 1295
EcoO109I RGGNCCY 1 cut(s) 201
EcoO65I GGTNACC 1 cut(s) 1295
EcoRII CCWGG 5 cut(s) 1080, 1806, 1951, 2236, 2552
EcoT14I CCWWGG 3 cut(s) 801, 1513, 2404
EcoT22I ATGCAT 1 cut(s) 1770
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 3 cut(s) 801, 1513, 2404
Esp3I CGTCTC 7 cut(s) 73, 142, 247, 266, 1274, 1381, 2233
FaeI CATG 9 cut(s) 805, 1100, 1371, 1523, 1772, 2123, 2141, 2256, 2408
FalI AAGNNNNNCTT 2 cut(s) 1199, 1231
FaqI GGGAC 4 cut(s) 34, 796, 1040, 2271
FatI CATG 9 cut(s) 801, 1096, 1367, 1519, 1768, 2119, 2137, 2252, 2404
FauI CCCGC 2 cut(s) 173, 241
FauNDI CATATG 1 cut(s) 2191
FbaI TGATCA 1 cut(s) 1738
FokI GGATG 8 cut(s) 418, 769, 1097, 1232, 1646, 2313, 2375, 2410
FriOI GRGCYC 1 cut(s) 234
GlaI GCGC 7 cut(s) 173, 305, 307, 427, 1182, 1562, 2153
HaeII RGCGCY 3 cut(s) 175, 429, 1564
HgaI GACGC 3 cut(s) 137, 397, 532
HhaI GCGC 7 cut(s) 174, 306, 308, 428, 1183, 1563, 2154
Hin1I GRCGYC 4 cut(s) 129, 216, 408, 1424
Hin1II CATG 9 cut(s) 805, 1100, 1371, 1523, 1772, 2123, 2141, 2256, 2408
Hin6I GCGC 7 cut(s) 172, 304, 306, 426, 1181, 1561, 2152
HinP1I GCGC 7 cut(s) 172, 304, 306, 426, 1181, 1561, 2152
HincII GTYRAC 1 cut(s) 798
HindII GTYRAC 1 cut(s) 798
HindIII AAGCTT 1 cut(s) 767
HphI GGTGA 9 cut(s) 745, 1256, 1285, 1307, 1586, 1733, 2258, 2575, 2600
Hpy166II GTNNAC 5 cut(s) 696, 798, 1594, 2026, 2369
Hpy8I GTNNAC 5 cut(s) 696, 798, 1594, 2026, 2369
HpyAV CCTTC 5 cut(s) 591, 715, 1002, 1036, 1285
HpyCH4III ACNGT 6 cut(s) 988, 1591, 2087, 2179, 2187, 2432
HpyCH4IV ACGT 4 cut(s) 216, 259, 1424, 2459
HpyF10VI GCNNNNNNNGC 8 cut(s) 276, 285, 310, 434, 570, 601, 630, 2394
HpyF3I CTNAG 8 cut(s) 281, 498, 1489, 1557, 1713, 2065, 2466, 2490
HpySE526I ACGT 4 cut(s) 216, 259, 1424, 2459
Hsp92I GRCGYC 4 cut(s) 129, 216, 408, 1424
Hsp92II CATG 9 cut(s) 805, 1100, 1371, 1523, 1772, 2123, 2141, 2256, 2408
HspAI GCGC 7 cut(s) 172, 304, 306, 426, 1181, 1561, 2152
Kpn2I TCCGGA 1 cut(s) 379
KroI GCCGGC 1 cut(s) 624
KroNI GCCGGC 1 cut(s) 626
Ksp22I TGATCA 1 cut(s) 1738
LmnI GCTCC 9 cut(s) 98, 478, 484, 490, 661, 1011, 1144, 1728, 1836
Lsp1109I GCAGC 3 cut(s) 415, 1771, 1826
LweI GCATC 8 cut(s) 440, 843, 1296, 1668, 1699, 1740, 2387, 2432
MaeII ACGT 4 cut(s) 216, 259, 1424, 2459
MbiI CCGCTC 2 cut(s) 330, 564
MflI RGATCY 2 cut(s) 222, 1355
MhlI GDGCHC 2 cut(s) 234, 1816
MlsI TGGCCA 1 cut(s) 1806
MluCI AATT 9 cut(s) 154, 508, 1132, 1327, 1929, 2412, 2448, 2592, 2608
MluNI TGGCCA 1 cut(s) 1806
MlyI GAGTC 3 cut(s) 56, 886, 2004
MmeI TCCRAC 7 cut(s) 132, 270, 321, 1017, 1415, 1801, 2451
Mox20I TGGCCA 1 cut(s) 1806
Mph1103I ATGCAT 1 cut(s) 1770
MroI TCCGGA 1 cut(s) 379
MroNI GCCGGC 1 cut(s) 624
MroXI GAANNNNTTC 1 cut(s) 2475
MscI TGGCCA 1 cut(s) 1806
MseI TTAA 4 cut(s) 771, 1206, 1668, 2632
MslI CAYNNNNRTG 1 cut(s) 1524
Msp20I TGGCCA 1 cut(s) 1806
MspA1I CMGCKG 2 cut(s) 285, 2576
MspCI CTTAAG 1 cut(s) 770
Mva1269I GAATGC 2 cut(s) 60, 913
MvaI CCWGG 5 cut(s) 1082, 1808, 1953, 2238, 2554
MvnI CGCG 4 cut(s) 270, 304, 306, 2152
MwoI GCNNNNNNNGC 8 cut(s) 276, 285, 310, 434, 570, 601, 630, 2394
NaeI GCCGGC 1 cut(s) 626
NciI CCSGG 5 cut(s) 13, 293, 320, 613, 787
NcoI CCATGG 2 cut(s) 801, 2404
NdeI CATATG 1 cut(s) 2191
NgoMIV GCCGGC 1 cut(s) 624
NlaIII CATG 9 cut(s) 805, 1100, 1371, 1523, 1772, 2123, 2141, 2256, 2408
NlaIV GGNNCC 7 cut(s) 202, 474, 480, 486, 784, 1049, 1825
NmeAIII GCCGAG 2 cut(s) 311, 517
NmuCI GTSAC 5 cut(s) 878, 988, 1295, 1777, 2207
NsiI ATGCAT 1 cut(s) 1770
NspV TTCGAA 1 cut(s) 905
PaqCI CACCTGC 1 cut(s) 1261
PauI GCGCGC 1 cut(s) 304
PcsI WCGNNNNNNNCGW 5 cut(s) 51, 117, 123, 132, 2503
PctI GAATGC 2 cut(s) 60, 913
PdiI GCCGGC 1 cut(s) 626
PdmI GAANNNNTTC 1 cut(s) 2475
PflFI GACNNNGTC 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 611
Ple19I CGATCG 1 cut(s) 58
PleI GAGTC 3 cut(s) 55, 885, 2003
PpsI GAGTC 3 cut(s) 55, 885, 2003
Psp6I CCWGG 5 cut(s) 1080, 1806, 1951, 2236, 2552
PspEI GGTNACC 1 cut(s) 1295
PspGI CCWGG 5 cut(s) 1080, 1806, 1951, 2236, 2552
PspN4I GGNNCC 7 cut(s) 202, 474, 480, 486, 784, 1049, 1825
PstI CTGCAG 2 cut(s) 1899, 1987
PstNI CAGNNNCTG 2 cut(s) 850, 1564
PsuI RGATCY 2 cut(s) 222, 1355
PsyI GACNNNGTC 1 cut(s) 407
PteI GCGCGC 1 cut(s) 304
PvuI CGATCG 1 cut(s) 58
RsaI GTAC 5 cut(s) 212, 697, 985, 1160, 1971
RsaNI GTAC 5 cut(s) 211, 696, 984, 1159, 1970
RseI CAYNNNNRTG 1 cut(s) 1524
SaqAI TTAA 4 cut(s) 771, 1206, 1668, 2632
ScaI AGTACT 2 cut(s) 985, 1971
SchI GAGTC 3 cut(s) 56, 886, 2004
SduI GDGCHC 2 cut(s) 234, 1816
SfaNI GCATC 8 cut(s) 440, 843, 1296, 1668, 1699, 1740, 2387, 2432
SfcI CTRYAG 3 cut(s) 1895, 1983, 2316
SfuI TTCGAA 1 cut(s) 905
SinI GGWCC 4 cut(s) 393, 578, 783, 1548
SmiMI CAYNNNNRTG 1 cut(s) 1524
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SpeI ACTAGT 2 cut(s) 1972, 2417
Sse9I AATT 9 cut(s) 154, 508, 1132, 1327, 1929, 2412, 2448, 2592, 2608
StyI CCWWGG 3 cut(s) 801, 1513, 2404
TaaI ACNGT 6 cut(s) 988, 1591, 2087, 2179, 2187, 2432
TaiI ACGT 4 cut(s) 219, 262, 1427, 2462
TaqI TCGA 5 cut(s) 54, 315, 411, 905, 2007
TasI AATT 9 cut(s) 154, 508, 1132, 1327, 1929, 2412, 2448, 2592, 2608
TatI WGTACW 4 cut(s) 695, 983, 1158, 1969
Tru1I TTAA 4 cut(s) 771, 1206, 1668, 2632
Tru9I TTAA 4 cut(s) 771, 1206, 1668, 2632
TscAI CASTG 6 cut(s) 422, 1021, 1075, 1741, 1786, 2226
TseFI GTSAC 5 cut(s) 878, 988, 1295, 1777, 2207
TseI GCWGC 3 cut(s) 428, 1784, 1839
Tsp45I GTSAC 5 cut(s) 878, 988, 1295, 1777, 2207
TspDTI ATGAA 7 cut(s) 1085, 1250, 1497, 1681, 2154, 2192, 2241
TspGWI ACGGA 4 cut(s) 229, 576, 1298, 1363
TspRI CASTG 6 cut(s) 422, 1021, 1075, 1741, 1786, 2226
Tth111I GACNNNGTC 1 cut(s) 407
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 4 cut(s) 393, 578, 783, 1548
XapI RAATTY 3 cut(s) 508, 2448, 2608
XbaI TCTAGA 1 cut(s) 1399
XmnI GAANNNNTTC 1 cut(s) 2475
ZraI GACGTC 2 cut(s) 217, 1425
ZrmI AGTACT 2 cut(s) 985, 1971
Zsp2I ATGCAT 1 cut(s) 1770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.