Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0011080

Physical Location & Seq

Genomic Coordinates Forward (+)

35181613 .. 35188403

Gene Structure 6791 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1241600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2631 bp

ATGAGCAGAGCCGGACAGGAAGAAGACGAAGACAACGAGTTCTACGAGTCCCTCGATCGCATTGTCTCCTCCTCTTGTTCTTGTTCCACTTCGAACTCCGACTCCGACCCCGACCCCGACCCCGACCCGAACCCGAATTATGCCGTACCCAAATTCCCAATGGGCGCCTCCACCAAGTACGATGTTTGGATCTCCGAGCCTTCCTCCGTCTCTGAACGCCGGTCCAAACTCCTCACTGAAATGGGTCTCACCGGCGACCCAGTGCTGACCCGGGCCAAACCCCAACTGGGTTTTTCCGGCGAGTTCGGGCGATCTGTCTCGTCGGATTATTTGATGACTGAAGTAAATTCCGGCGGTGGAGGCGTTAATAGCATCGTCCGCTCCAAATCCGACGGCGGCGATCAGTGTAATAACGCTTGTTCTACTTCATCTGTTTCTTCTCCCCCATTTCTTTCGGTTCGTTGTGGTTCCAGTGAGGCCGAGACCGAGCCTGAACCCGAAACCGGTCCATTTGTAAATAGAAATACAAAATCTTTGGTTTGTAAATCTTCAAGCGGTAAAAGCAACTCATCTCCGCCACCGAATAAGCCGCCTTCTGGCAAGAATTCGAGGAGGGTAGACGAGATTCGAAGTGATTCGAAGGCGGAGGAGTTGGATTGCAATGACATTGGTAAGGTTAGTGAAAGTGATGCTATTAATAATGCCCAAGTGTGTACTATTAGGAACCTTGATAATGGTAAAGAGTTTGTTGTGAATGAGATTAGAGAAGATGGAATGTGGAACAAGCTTAAGGAAGTGGGTACAGGGAAGCAGTTGACCATGGAGGAGTTTGAGATGAGCGTCGGGCATTCACCGATTGTTCAAGAACTGATGAGGAGGCAGAACGTGGAGGAGGGTCATAAGGATGGTTCCGAGTCCAATGCCAATGGGAGCAATGACGGGGTTTCAAAGTTGAAGAAGAAAGGGGGTTGGTTGAAGAGCATCAAGAGTGTTGCGATCACCATGACGGGTCACAAGGATAGGCGGAGTAGCGATGACAGGGATACATCATCGGAAAAGGGAGGTCGGAGGTCAAGCTCTGGCACAGATGATAGCCAGGATGTGTCGTTTCATGGGCCGGAGAGGGTGAGGGTGAGGCAGTATGGGAAGTCATGTAAGGAGGTCACTGCATTGTTCAAGAGTCAGGAGATTCAGGCGCATACTGGGTCGATTTGGAGCATTAAATTTAGTTTGGATGGAAAATATTTGGCTAGTGCCGGTGAGGATTGCGTGATTCATATTTGGCAGGTGATGCAGAGTGAGAGGAAGGGTGACCTGTTGATGGAGAAGTCAGAAGATAGCAATTTAAACATGTTGTTATTTGCCAATGGGTCGCCGGAAGCATGTTCAGTCTCACCGAATGTGGACAACCTTGTGGAAAAAAAGAGGAGAGGAAGGTCGTCTATCGGTCGAAAATCTGTTAGTTTGGACCATTATGTGATTCCAGAGACTGTGTTTGCACTTTCAGAGAAACCCATTTCTTCATTCCAAGGGCATCTGGATGATGTGCTTGACCTTTCTTGGTCCAAGTCGCAGCACCTGCTCTCATCTTCAATGGACAAAACTGTGCGCCTTTGGCACTTGGCGAGCAAGACTTGTCTGAAGATATTTTCACATAGTGACTATGTAACTTGCATCCAGTTCAACCCTGTTGATGATAGGTACTTCATTAGTGGATCACTTGATGCTAAGGTTCGCATATGGAGCATCCCTGATCGTCAAGTGGTTGATTGGAATGATCTGCACGAGATGGTCACTGCTGCTTGCTACACTCCTGATGGTCAGGGCGCCCTAGTTGGTTCATACAAAGGCAGCTGCCGTTTATACAATACATCTGAGAACAAGTTGCAACAGAAAAGCCAAATTAATCTGCAGAACAAGAAAAAGAAATCACATCACAAGAAAATTACAGGTTTTCAGTTTGCACCAGGAAGTTCATCAGAAGTACTGATTACGTCTGCAGATTCACGAATTAGAGTTGTTGACGGTATTGATCTGGTTCACAAGTTCAAGGGATTTCGGAACGCAAATAGCCAAATCTCAGCCACCCTCACGGCAAATGGTAAATATGTGGTATCTGCTAGTGAGGATTCTCATGTATATATATGGAAACATGAAGCTGATTCCCGTCCTAGCAGAAGCAAAAGTGTCACTGTCACACGGTCCTTTGAGCATTTCCATTGTCAAGATGTATCAGCAGCCATCCCTTGGCCTGGAATGACTGATTTATGGGGATTACAAGATTCAGAACGTAATGGGCTTGACGAGGTCCCCACAGCAAATCACCCCCCTACACCAGTTGAGGTGGCTAATGCCAACGAGGGTTCAAGATCAGCATCTGGTTGCACCAATAGCCCTCTCCACGGAATCATTTCGAGTGCATCCAACAATTACTTCTTTGATAGAATTTCAGCAACGTGGCCGGAGGAAAAACTTCTTCTAGCTACTAGGAACCGAAGCCCACGTGTAAGTTTTGATTTTACGAATGGTTTAAGCCAAAACATGTCAGCCTGGGGTGTGGTGATTGTAACTGCTGGCCTTCGAGGTGAAATTAGAACTTTCCAAAATTTTGGGTTGCCGATTCGGATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0010008 GO:0012505 GO:0016020 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048471 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

877

Amino Acids

96.28

Weight (kDa)

6.28

Isoelectric Point (pI)

50.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	392 - 431	1.6e-07	THOC3 beta-propeller domain
WD40	PF00400	395 - 428	9.4e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	397 - 439	1e-06	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	486 - 566	3.3e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	500 - 720	8.3e-37	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	502 - 583	1.3e-12	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	502 - 630	6.4e-15	Prp19 WD40 domain
EIF3I	PF24805	503 - 705	4.8e-09	EIF3I
WD40	PF00400	504 - 539	2.5e-07	WD domain, G-beta repeat
WD40_CDC20-Fz	PF24807	504 - 589	2.5e-08	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	506 - 549	8.5e-06	THOC3 beta-propeller domain
Beta-prop_IFT122_1st	PF23381	508 - 625	1.4e-06	IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	509 - 588	1.3e-11	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	513 - 719	1.4e-23	THOC3 beta-propeller domain
Beta-prop_Aladin	PF25460	514 - 634	4.1e-09	Aladin seven-bladed propeller
Beta-prop_WDR3_2nd	PF25172	515 - 716	4.6e-12	WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd	PF23393	524 - 630	1.3e-08	WDR90/POC16, second beta-propeller
WD40_Gbeta	PF25391	525 - 716	6.2e-09	G protein beta WD-40 repeat protein
WD40	PF00400	545 - 582	8.3e-06	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	553 - 724	4.7e-12	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	567 - 724	3e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	587 - 718	4.3e-08	WDR3 first beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15470 AT5G54200
fragaria_vesca	FvH4_3g20610 FvH4_3g20610
malus_domestica	MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica	Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis	pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis	RchiOBHm_Chr5g0034631
rosa_laevigata	RLG00000033560
rosa_roxburghii	Rroxscaffold_1G00045690
rosa_rugosa	Rorug05G0147300
rosa_samantha	Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana	Rw5G021930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1276, 1587
Acc36I	ACCTGC	2 cut(s)	1276, 1587
AccB1I	GGYRCC	2 cut(s)	164, 1826
AccB7I	CCANNNNNTGG	1 cut(s)	2247
AccBSI	CCGCTC	1 cut(s)	381
AccI	GTMKAC	1 cut(s)	618
AciI	CCGC	8 cut(s)	354, 379, 396, 555, 575, 590, 644, 1024
AclWI	GGATC	2 cut(s)	197, 1723
AcoI	YGGCCR	1 cut(s)	2459
AcsI	RAATTY	6 cut(s)	152, 346, 604, 1223, 2445, 2605
AcuI	CTGAAG	2 cut(s)	360, 1661
AcvI	CACGTG	1 cut(s)	2504
AcyI	GRCGYC	2 cut(s)	165, 1827
AdeI	CACNNNGTG	1 cut(s)	1658
AfaI	GTAC	6 cut(s)	147, 179, 715, 802, 1703, 1986
AfiI	CCNNNNNNNGG	7 cut(s)	287, 503, 596, 1321, 2247, 2252, 2402
AflII	CTTAAG	1 cut(s)	788
AflIII	ACRYGT	3 cut(s)	1350, 2503, 2541
AgeI	ACCGGT	1 cut(s)	503
AjnI	CCWGG	4 cut(s)	1095, 1966, 2251, 2549
AjuI	GAANNNNNNNTTGG	2 cut(s)	2417, 2449
AloI	GAACNNNNNNTCC	2 cut(s)	86, 118
AluBI	AGCT	5 cut(s)	787, 1077, 1854, 2159, 2483
AluI	AGCT	5 cut(s)	787, 1077, 1854, 2159, 2483
Alw26I	GTCTC	7 cut(s)	70, 214, 251, 322, 476, 1396, 1481
AlwI	GGATC	2 cut(s)	197, 1723
AlwNI	CAGNNNCTG	3 cut(s)	1490, 1579, 2378
Ama87I	CYCGRG	1 cut(s)	270
AoxI	GGCC	6 cut(s)	273, 477, 1115, 2249, 2459, 2575
ApeKI	GCWGC	5 cut(s)	1573, 1799, 1851, 1854, 2237
ApoI	RAATTY	6 cut(s)	152, 346, 604, 1223, 2445, 2605
AseI	ATTAAT	2 cut(s)	696, 1905
AsiGI	ACCGGT	1 cut(s)	503
Asp700I	GAANNNNTTC	3 cut(s)	634, 2410, 2472
AspLEI	GCGC	4 cut(s)	167, 1198, 1611, 1829
AspS9I	GGNCC	8 cut(s)	222, 273, 506, 1115, 1468, 1563, 2202, 2308
AsuC2I	CCSGG	2 cut(s)	271, 272
AsuII	TTCGAA	3 cut(s)	92, 628, 638
AvaI	CYCGRG	1 cut(s)	270
AvaII	GGWCC	6 cut(s)	222, 506, 1468, 1563, 2202, 2308
BanI	GGYRCC	2 cut(s)	164, 1826
BauI	CACGAG	1 cut(s)	1784
BbrPI	CACGTG	1 cut(s)	2504
BbsI	GAAGAC	2 cut(s)	30, 36
BbvI	GCAGC	5 cut(s)	1585, 1786, 1841, 1863, 2249
BccI	CCATC	7 cut(s)	764, 899, 1229, 1315, 1783, 1811, 2249
BceAI	ACGGC	4 cut(s)	128, 409, 1842, 2109
BcgI	CGANNNNNNTGC	2 cut(s)	902, 936
BciT130I	CCWGG	4 cut(s)	1097, 1968, 2253, 2551
BciVI	GTATCC	1 cut(s)	1036
BcnI	CCSGG	2 cut(s)	271, 272
BcoDI	GTCTC	7 cut(s)	70, 214, 251, 322, 476, 1396, 1481
BfaI	CTAG	6 cut(s)	1251, 1832, 2121, 2172, 2480, 2487
BfmI	CTRYAG	2 cut(s)	1910, 1998
BfoI	RGCGCY	2 cut(s)	168, 1830
BfrI	CTTAAG	1 cut(s)	788
BfuAI	ACCTGC	2 cut(s)	1276, 1587
BfuI	GTATCC	1 cut(s)	1036
BisI	GCNGC	7 cut(s)	397, 590, 1574, 1800, 1852, 1855, 2238
BlsI	GCNGC	7 cut(s)	398, 591, 1575, 1801, 1853, 1856, 2239
BmcAI	AGTACT	1 cut(s)	1986
Bme1390I	CCNGG	6 cut(s)	271, 272, 1097, 1968, 2253, 2551
Bme18I	GGWCC	6 cut(s)	222, 506, 1468, 1563, 2202, 2308
BmeT110I	CYCGRG	1 cut(s)	270
BmgT120I	GGNCC	8 cut(s)	222, 273, 506, 1115, 1468, 1563, 2202, 2308
BmiI	GGNNCC	7 cut(s)	166, 469, 725, 910, 1828, 2310, 2492
BmrFI	CCNGG	6 cut(s)	271, 272, 1097, 1968, 2253, 2551
BmrI	ACTGGG	3 cut(s)	254, 296, 1212
BmuI	ACTGGG	3 cut(s)	254, 296, 1212
BpiI	GAAGAC	2 cut(s)	30, 36
BplI	GAGNNNNNCTC	2 cut(s)	188, 220
Bpu10I	CCTNAGC	1 cut(s)	1728
Bpu14I	TTCGAA	3 cut(s)	92, 628, 638
BpuMI	CCSGG	2 cut(s)	271, 272
BsaAI	YACGTR	1 cut(s)	2504
BsaBI	GATNNNNATC	1 cut(s)	2374
BsaHI	GRCGYC	2 cut(s)	165, 1827
BsaI	GGTCTC	2 cut(s)	251, 476
BsaJI	CCNNGG	6 cut(s)	270, 819, 1528, 2246, 2401, 2550
BsaWI	WCCGGW	1 cut(s)	503
BsaXI	ACNNNNNCTCC	2 cut(s)	86, 116
Bsc4I	CCNNNNNNNGG	7 cut(s)	287, 503, 596, 1321, 2247, 2252, 2402
Bse118I	RCCGGY	4 cut(s)	219, 251, 503, 1256
Bse1I	ACTGG	6 cut(s)	260, 291, 471, 1207, 1678, 2336
Bse3DI	GCAATG	2 cut(s)	667, 940
Bse8I	GATNNNNATC	1 cut(s)	2374
BseBI	CCWGG	4 cut(s)	1097, 1968, 2253, 2551
BseDI	CCNNGG	6 cut(s)	270, 819, 1528, 2246, 2401, 2550
BseGI	GGATG	8 cut(s)	910, 1105, 1240, 1546, 1674, 1746, 2241, 2420
BseJI	GATNNNNATC	1 cut(s)	2374
BseLI	CCNNNNNNNGG	7 cut(s)	287, 503, 596, 1321, 2247, 2252, 2402
BseMI	GCAATG	2 cut(s)	667, 940
BseMII	CTCAG	2 cut(s)	1866, 2094
BseNI	ACTGG	6 cut(s)	260, 291, 471, 1207, 1678, 2336
BseRI	GAGGAG	9 cut(s)	58, 61, 221, 625, 662, 839, 889, 905, 1441
BseXI	GCAGC	5 cut(s)	1585, 1786, 1841, 1863, 2249
BsgI	GTGCAG	1 cut(s)	1766
Bsh1285I	CGRYCG	2 cut(s)	58, 1450
BshFI	GGCC	6 cut(s)	275, 479, 1117, 2251, 2461, 2577
BshNI	GGYRCC	2 cut(s)	164, 1826
BshTI	ACCGGT	1 cut(s)	503
BsiEI	CGRYCG	2 cut(s)	58, 1450
BsiHKCI	CYCGRG	1 cut(s)	270
BslFI	GGGAC	2 cut(s)	34, 2294
BslI	CCNNNNNNNGG	7 cut(s)	287, 503, 596, 1321, 2247, 2252, 2402
BsmAI	GTCTC	7 cut(s)	70, 214, 251, 322, 476, 1396, 1481
BsmBI	CGTCTC	1 cut(s)	214
BsmFI	GGGAC	2 cut(s)	34, 2294
BsmI	GAATGC	1 cut(s)	847
BsnI	GGCC	6 cut(s)	275, 479, 1117, 2251, 2461, 2577
Bso31I	GGTCTC	2 cut(s)	251, 476
BsoBI	CYCGRG	1 cut(s)	270
Bsp119I	TTCGAA	3 cut(s)	92, 628, 638
Bsp19I	CCATGG	1 cut(s)	819
BspACI	CCGC	8 cut(s)	354, 379, 396, 555, 575, 590, 644, 1024
BspANI	GGCC	6 cut(s)	275, 479, 1117, 2251, 2461, 2577
BspCNI	CTCAG	2 cut(s)	1867, 2093
BspLI	GGNNCC	7 cut(s)	166, 469, 725, 910, 1828, 2310, 2492
BspMAI	CTGCAG	2 cut(s)	1914, 2002
BspMI	ACCTGC	2 cut(s)	1276, 1587
BspPI	GGATC	2 cut(s)	197, 1723
BspQI	GCTCTTC	1 cut(s)	971
BspT104I	TTCGAA	3 cut(s)	92, 628, 638
BspT107I	GGYRCC	2 cut(s)	164, 1826
BspTI	CTTAAG	1 cut(s)	788
BspTNI	GGTCTC	2 cut(s)	251, 476
BsrBI	CCGCTC	1 cut(s)	381
BsrDI	GCAATG	2 cut(s)	667, 940
BsrFI	RCCGGY	4 cut(s)	219, 251, 503, 1256
BsrI	ACTGG	6 cut(s)	260, 291, 471, 1207, 1678, 2336
BssAI	RCCGGY	4 cut(s)	219, 251, 503, 1256
BssECI	CCNNGG	6 cut(s)	270, 819, 1528, 2246, 2401, 2550
BssNI	GRCGYC	2 cut(s)	165, 1827
BssSI	CACGAG	1 cut(s)	1784
BssT1I	CCWWGG	3 cut(s)	819, 1528, 2246
Bst2BI	CACGAG	1 cut(s)	1784
Bst2UI	CCWGG	4 cut(s)	1097, 1968, 2253, 2551
Bst4CI	ACNGT	5 cut(s)	1492, 1606, 2027, 2194, 2202
Bst6I	CTCTTC	1 cut(s)	971
BstACI	GRCGYC	2 cut(s)	165, 1827
BstAFI	CTTAAG	1 cut(s)	788
BstAPI	GCANNNNNTGC	2 cut(s)	1291, 1579
BstBAI	YACGTR	1 cut(s)	2504
BstBI	TTCGAA	3 cut(s)	92, 628, 638
BstC8I	GCNNGC	3 cut(s)	1627, 1804, 2575
BstDEI	CTNAG	3 cut(s)	1728, 1875, 2080
BstDSI	CCRYGG	2 cut(s)	819, 2401
BstEII	GGTNACC	1 cut(s)	1310
BstF5I	GGATG	8 cut(s)	910, 1105, 1240, 1546, 1674, 1746, 2241, 2420
BstH2I	RGCGCY	2 cut(s)	168, 1830
BstHHI	GCGC	4 cut(s)	167, 1198, 1611, 1829
BstMAI	GTCTC	7 cut(s)	70, 214, 251, 322, 476, 1396, 1481
BstMCI	CGRYCG	2 cut(s)	58, 1450
BstMWI	GCNNNNNNNGC	9 cut(s)	360, 369, 378, 561, 1291, 1579, 1615, 1743, 2391
BstNI	CCWGG	4 cut(s)	1097, 1968, 2253, 2551
BstNSI	RCATGY	3 cut(s)	1354, 1386, 2545
BstPI	GGTNACC	1 cut(s)	1310
BstSCI	CCNGG	6 cut(s)	269, 270, 1095, 1966, 2251, 2549
BstSFI	CTRYAG	2 cut(s)	1910, 1998
BstV1I	GCAGC	5 cut(s)	1585, 1786, 1841, 1863, 2249
BstV2I	GAAGAC	2 cut(s)	30, 36
BstX2I	RGATCY	1 cut(s)	189
BstXI	CCANNNNNNTGG	1 cut(s)	2609
BstYI	RGATCY	1 cut(s)	189
BsuI	GTATCC	1 cut(s)	1036
BsuRI	GGCC	6 cut(s)	275, 479, 1117, 2251, 2461, 2577
BtgI	CCRYGG	2 cut(s)	819, 2401
BtgZI	GCGATG	1 cut(s)	1047
BtsCI	GGATG	8 cut(s)	910, 1105, 1240, 1546, 1674, 1746, 2241, 2420
BtsI	GCAGTG	2 cut(s)	1164, 1794
BtsIMutI	CAGTG	7 cut(s)	234, 267, 410, 478, 1164, 1794, 2190
BveI	ACCTGC	2 cut(s)	1276, 1587
Cac8I	GCNNGC	3 cut(s)	1627, 1804, 2575
CaiI	CAGNNNCTG	3 cut(s)	1490, 1579, 2378
CfoI	GCGC	4 cut(s)	167, 1198, 1611, 1829
Cfr10I	RCCGGY	4 cut(s)	219, 251, 503, 1256
Cfr13I	GGNCC	8 cut(s)	222, 273, 506, 1115, 1468, 1563, 2202, 2308
Cfr9I	CCCGGG	1 cut(s)	270
CseI	GACGC	1 cut(s)	829
Csp6I	GTAC	6 cut(s)	146, 178, 714, 801, 1702, 1985
CspAI	ACCGGT	1 cut(s)	503
CviAII	CATG	9 cut(s)	820, 1003, 1112, 1152, 1351, 1383, 2135, 2153, 2542
CviQI	GTAC	6 cut(s)	146, 178, 714, 801, 1702, 1985
DdeI	CTNAG	3 cut(s)	1728, 1875, 2080
DinI	GGCGCC	2 cut(s)	166, 1828
DraI	TTTAAA	1 cut(s)	1347
DraIII	CACNNNGTG	1 cut(s)	1658
EaeI	YGGCCR	1 cut(s)	2459
Eam1104I	CTCTTC	1 cut(s)	971
EarI	CTCTTC	1 cut(s)	971
EciI	GGCGGA	3 cut(s)	564, 659, 1039
Eco130I	CCWWGG	3 cut(s)	819, 1528, 2246
Eco31I	GGTCTC	2 cut(s)	251, 476
Eco47I	GGWCC	6 cut(s)	222, 506, 1468, 1563, 2202, 2308
Eco57I	CTGAAG	2 cut(s)	360, 1661
Eco72I	CACGTG	1 cut(s)	2504
Eco88I	CYCGRG	1 cut(s)	270
Eco91I	GGTNACC	1 cut(s)	1310
EcoO109I	RGGNCCY	1 cut(s)	2308
EcoO65I	GGTNACC	1 cut(s)	1310
EcoRI	GAATTC	1 cut(s)	604
EcoRII	CCWGG	4 cut(s)	1095, 1966, 2251, 2549
EcoT14I	CCWWGG	3 cut(s)	819, 1528, 2246
EgeI	GGCGCC	2 cut(s)	166, 1828
EheI	GGCGCC	2 cut(s)	166, 1828
ErhI	CCWWGG	3 cut(s)	819, 1528, 2246
Esp3I	CGTCTC	1 cut(s)	214
FaeI	CATG	9 cut(s)	823, 1006, 1115, 1155, 1354, 1386, 2138, 2156, 2545
FaqI	GGGAC	2 cut(s)	34, 2294
FatI	CATG	9 cut(s)	819, 1002, 1111, 1151, 1350, 1382, 2134, 2152, 2541
FauNDI	CATATG	1 cut(s)	1739
FblI	GTMKAC	1 cut(s)	618
Fnu4HI	GCNGC	7 cut(s)	397, 590, 1574, 1800, 1852, 1855, 2238
FokI	GGATG	8 cut(s)	917, 1112, 1247, 1553, 1661, 1733, 2228, 2407
Fsp4HI	GCNGC	7 cut(s)	397, 590, 1574, 1800, 1852, 1855, 2238
FspBI	CTAG	6 cut(s)	1251, 1832, 2121, 2172, 2480, 2487
GlaI	GCGC	4 cut(s)	166, 1197, 1610, 1828
GluI	GCNGC	7 cut(s)	397, 590, 1574, 1800, 1852, 1855, 2238
HaeII	RGCGCY	2 cut(s)	168, 1830
HaeIII	GGCC	6 cut(s)	275, 479, 1117, 2251, 2461, 2577
HgaI	GACGC	1 cut(s)	829
HhaI	GCGC	4 cut(s)	167, 1198, 1611, 1829
Hin1I	GRCGYC	2 cut(s)	165, 1827
Hin1II	CATG	9 cut(s)	823, 1006, 1115, 1155, 1354, 1386, 2138, 2156, 2545
Hin6I	GCGC	4 cut(s)	165, 1196, 1609, 1827
HinP1I	GCGC	4 cut(s)	165, 1196, 1609, 1827
HincII	GTYRAC	2 cut(s)	816, 2023
HindII	GTYRAC	2 cut(s)	816, 2023
HindIII	AAGCTT	1 cut(s)	785
Hpy166II	GTNNAC	6 cut(s)	619, 714, 816, 1405, 2023, 2041
Hpy8I	GTNNAC	6 cut(s)	619, 714, 816, 1405, 2023, 2041
Hpy99I	CGWCG	3 cut(s)	325, 395, 845
HpyAV	CCTTC	6 cut(s)	210, 603, 634, 1300, 1428, 2588
HpyCH4III	ACNGT	5 cut(s)	1492, 1606, 2027, 2194, 2202
HpyCH4IV	ACGT	5 cut(s)	885, 1994, 2290, 2456, 2503
HpyF10VI	GCNNNNNNNGC	9 cut(s)	360, 369, 378, 561, 1291, 1579, 1615, 1743, 2391
HpyF3I	CTNAG	3 cut(s)	1728, 1875, 2080
HpySE526I	ACGT	5 cut(s)	885, 1994, 2290, 2456, 2503
Hsp92I	GRCGYC	2 cut(s)	165, 1827
Hsp92II	CATG	9 cut(s)	823, 1006, 1115, 1155, 1354, 1386, 2138, 2156, 2545
HspAI	GCGC	4 cut(s)	165, 1196, 1609, 1827
KasI	GGCGCC	2 cut(s)	164, 1826
LguI	GCTCTTC	1 cut(s)	971
LmnI	GCTCC	4 cut(s)	386, 930, 1215, 1743
Lsp1109I	GCAGC	5 cut(s)	1585, 1786, 1841, 1863, 2249
MaeI	CTAG	6 cut(s)	1251, 1832, 2121, 2172, 2480, 2487
MaeII	ACGT	5 cut(s)	885, 1994, 2290, 2456, 2503
MaeIII	GTNAC	9 cut(s)	1010, 1162, 1310, 1658, 1666, 1792, 2188, 2194, 2566
MbiI	CCGCTC	1 cut(s)	381
MflI	RGATCY	1 cut(s)	189
Mly113I	GGCGCC	2 cut(s)	165, 1827
MlyI	GAGTC	4 cut(s)	56, 95, 923, 1189
MmeI	TCCRAC	7 cut(s)	123, 129, 303, 414, 633, 1046, 2448
MroXI	GAANNNNTTC	3 cut(s)	634, 2410, 2472
MseI	TTAA	8 cut(s)	366, 696, 789, 1221, 1346, 1905, 2531, 2629
MslI	CAYNNNNRTG	3 cut(s)	239, 903, 1539
MspA1I	CMGCKG	1 cut(s)	1854
MspCI	CTTAAG	1 cut(s)	788
MspR9I	CCNGG	6 cut(s)	271, 272, 1097, 1968, 2253, 2551
Mva1269I	GAATGC	1 cut(s)	847
MvaI	CCWGG	4 cut(s)	1097, 1968, 2253, 2551
MwoI	GCNNNNNNNGC	9 cut(s)	360, 369, 378, 561, 1291, 1579, 1615, 1743, 2391
NarI	GGCGCC	2 cut(s)	165, 1827
NciI	CCSGG	2 cut(s)	271, 272
NcoI	CCATGG	1 cut(s)	819
NdeI	CATATG	1 cut(s)	1739
NlaIII	CATG	9 cut(s)	823, 1006, 1115, 1155, 1354, 1386, 2138, 2156, 2545
NlaIV	GGNNCC	7 cut(s)	166, 469, 725, 910, 1828, 2310, 2492
NmeAIII	GCCGAG	1 cut(s)	505
NmuCI	GTSAC	7 cut(s)	1010, 1162, 1310, 1658, 1792, 2188, 2194
NspI	RCATGY	3 cut(s)	1354, 1386, 2545
NspV	TTCGAA	3 cut(s)	92, 628, 638
PaqCI	CACCTGC	2 cut(s)	1276, 1587
PciI	ACATGT	2 cut(s)	1350, 2541
PciSI	GCTCTTC	1 cut(s)	971
PcsI	WCGNNNNNNNCGW	3 cut(s)	33, 42, 51
PctI	GAATGC	1 cut(s)	847
PdmI	GAANNNNTTC	3 cut(s)	634, 2410, 2472
PflFI	GACNNNGTC	1 cut(s)	2306
PflMI	CCANNNNNTGG	1 cut(s)	2247
PinAI	ACCGGT	1 cut(s)	503
PkrI	GCNGC	7 cut(s)	398, 591, 1575, 1801, 1853, 1856, 2239
Ple19I	CGATCG	1 cut(s)	58
PleI	GAGTC	4 cut(s)	55, 95, 922, 1188
PluTI	GGCGCC	2 cut(s)	168, 1830
PmaCI	CACGTG	1 cut(s)	2504
PmlI	CACGTG	1 cut(s)	2504
PpsI	GAGTC	4 cut(s)	55, 95, 922, 1188
Ppu21I	YACGTR	1 cut(s)	2504
PpuMI	RGGWCCY	1 cut(s)	2308
PscI	ACATGT	2 cut(s)	1350, 2541
PshBI	ATTAAT	2 cut(s)	696, 1905
Psp5II	RGGWCCY	1 cut(s)	2308
Psp6I	CCWGG	4 cut(s)	1095, 1966, 2251, 2549
PspCI	CACGTG	1 cut(s)	2504
PspEI	GGTNACC	1 cut(s)	1310
PspGI	CCWGG	4 cut(s)	1095, 1966, 2251, 2549
PspN4I	GGNNCC	7 cut(s)	166, 469, 725, 910, 1828, 2310, 2492
PspPI	GGNCC	8 cut(s)	222, 273, 506, 1115, 1468, 1563, 2202, 2308
PspPPI	RGGWCCY	1 cut(s)	2308
PstI	CTGCAG	2 cut(s)	1914, 2002
PstNI	CAGNNNCTG	3 cut(s)	1490, 1579, 2378
PsuI	RGATCY	1 cut(s)	189
PsyI	GACNNNGTC	1 cut(s)	2306
PvuI	CGATCG	1 cut(s)	58
PvuII	CAGCTG	1 cut(s)	1854
RsaI	GTAC	6 cut(s)	147, 179, 715, 802, 1703, 1986
RsaNI	GTAC	6 cut(s)	146, 178, 714, 801, 1702, 1985
RseI	CAYNNNNRTG	3 cut(s)	239, 903, 1539
SapI	GCTCTTC	1 cut(s)	971
SaqAI	TTAA	8 cut(s)	366, 696, 789, 1221, 1346, 1905, 2531, 2629
SatI	GCNGC	7 cut(s)	397, 590, 1574, 1800, 1852, 1855, 2238
Sau96I	GGNCC	8 cut(s)	222, 273, 506, 1115, 1468, 1563, 2202, 2308
ScaI	AGTACT	1 cut(s)	1986
SchI	GAGTC	4 cut(s)	56, 95, 923, 1189
ScrFI	CCNGG	6 cut(s)	271, 272, 1097, 1968, 2253, 2551
SfcI	CTRYAG	2 cut(s)	1910, 1998
SfoI	GGCGCC	2 cut(s)	166, 1828
SfuI	TTCGAA	3 cut(s)	92, 628, 638
SgrAI	CRCCGGYG	1 cut(s)	251
SinI	GGWCC	6 cut(s)	222, 506, 1468, 1563, 2202, 2308
SmaI	CCCGGG	1 cut(s)	272
SmiMI	CAYNNNNRTG	3 cut(s)	239, 903, 1539
SmlI	CTYRAG	1 cut(s)	788
SmoI	CTYRAG	1 cut(s)	788
SsiI	CCGC	8 cut(s)	354, 379, 396, 555, 575, 590, 644, 1024
SspDI	GGCGCC	2 cut(s)	164, 1826
SspI	AATATT	1 cut(s)	1244
SspMI	CTAG	6 cut(s)	1251, 1832, 2121, 2172, 2480, 2487
StyD4I	CCNGG	6 cut(s)	269, 270, 1095, 1966, 2251, 2549
StyI	CCWWGG	3 cut(s)	819, 1528, 2246
TaaI	ACNGT	5 cut(s)	1492, 1606, 2027, 2194, 2202
TaiI	ACGT	5 cut(s)	888, 1997, 2293, 2459, 2506
TaqI	TCGA	9 cut(s)	54, 92, 608, 628, 638, 1208, 1450, 2414, 2581
TaqII	GACCGA	2 cut(s)	500, 1436
TatI	WGTACW	2 cut(s)	713, 1984
TauI	GCSGC	2 cut(s)	399, 592
Tru1I	TTAA	8 cut(s)	366, 696, 789, 1221, 1346, 1905, 2531, 2629
Tru9I	TTAA	8 cut(s)	366, 696, 789, 1221, 1346, 1905, 2531, 2629
TscAI	CASTG	7 cut(s)	241, 267, 410, 478, 1171, 1801, 2197
TseFI	GTSAC	7 cut(s)	1010, 1162, 1310, 1658, 1792, 2188, 2194
TseI	GCWGC	5 cut(s)	1573, 1799, 1851, 1854, 2237
Tsp45I	GTSAC	7 cut(s)	1010, 1162, 1310, 1658, 1792, 2188, 2194
TspDTI	ATGAA	8 cut(s)	417, 1100, 1265, 1512, 1696, 1830, 1965, 2169
TspGWI	ACGGA	2 cut(s)	196, 2418
TspMI	CCCGGG	1 cut(s)	270
TspRI	CASTG	7 cut(s)	241, 267, 410, 478, 1171, 1801, 2197
Tth111I	GACNNNGTC	1 cut(s)	2306
Van91I	CCANNNNNTGG	1 cut(s)	2247
Vha464I	CTTAAG	1 cut(s)	788
VpaK11BI	GGWCC	6 cut(s)	222, 506, 1468, 1563, 2202, 2308
VspI	ATTAAT	2 cut(s)	696, 1905
XapI	RAATTY	6 cut(s)	152, 346, 604, 1223, 2445, 2605
XceI	RCATGY	3 cut(s)	1354, 1386, 2545
XcmI	CCANNNNNNNNNTGG	2 cut(s)	157, 283
XmaI	CCCGGG	1 cut(s)	270
XmiI	GTMKAC	1 cut(s)	618
XmnI	GAANNNNTTC	3 cut(s)	634, 2410, 2472
XspI	CTAG	6 cut(s)	1251, 1832, 2121, 2172, 2480, 2487
ZrmI	AGTACT	1 cut(s)	1986

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.