MD03G1223700.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011080
Physical Location & Seq
Forward (+)
30892758 .. 30899244
6487 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1223700.v1.1.491

Sequence Viewer

Length: 2634 bp
ATGAGCAGAGCCGGAGAGGAAGAAGACAAAGACAGCGAGTGCTTCTACGAGTCCCTCGATCGCATTGTCTCCTCCTCTTGTTCTTGTTCAACATCGAACTCCGACTCCGAGGCCGACCCCGACCCGAATCCGAATTATGCCGTACCCAAATTCCCAATGGGCGCCTCCACCAAGTACGACGTTTGGACCTCCGAGCCTTCCTCCATCTCCGAACGCCGGTCTAAACTCCTCACCGAAATGGGTCTCACCGGCGACCCGGTCCTGACCCGGGCCAAGCCTCAACTGGGTTTTGCCGGTGAGTTCGGGCGGTCGGTCTCGTCGGATTATTTGATCGCTCAAGTGAATTCCGACGGTGGTGGCGTTAATAGCATTGTCCGCTCCAAATCCGACGGCAGCGATCAGTGTAATAACGCTTGTTCTACTTCATCTGTTTCTTCTCCCCCAATTCTTTCGATTCGTTGTGGTTCTGGCGAGGCACAGACCGAGCCTGAACCCGAAACCGGTTCATTTGTAAATAGAAATACAAAATCTTTGGATTATAAATCTTCAAGTGGTAAAAGCAACTCATCTCCGCCGCCGAATAAGCCGCCTTCCGGCAAGCACTCGAGGAGGGTAGACGAGATTCAAAGCGATTCGAAGGCAGAGGAGGAGTTGGATTGCAATGGCATTGTTAAGGTTAGTGAAAGTGATGCTAACAATGATGCCCAAGTGTGTACTATTAGGAACCTTGATAATGGGAAAGAGTTTGTTGTGAATGAGATTAGGGAAGATGGAACGTGGAACAAGCTTAAGGAAGTGGGTACTGGAAAGCAGTTGACCATGGAGGAGTTTGAGATGAGTGTCGGGCATTCACCGATTGTTCAAGAACTGATGAGGAGGCAGAATGTGGAAGAGGGTCATAAGGATGGTTCCGAGACCAATGCCAATGGGGGGAATGGTGGGGTTTCGAAGTTGAAGAAGAAAGGAGGCTGGTTAAAGAGCATCAAGAGTGTCGCGATCACCATGACAGGTCATAAGGATAGGCGGAGTAGCGATGAGAGGGACACATCATCGGAGAAAGGTGGTCGGAGGTCGAGCTCTGCCACAGATGATAGCCAGGATGTGTCATTTCATGGGCCGGAGAGGGTGAGGGTGAAGCAGTATGGGAAGTCATGTAAGGAGGTCACTGCATTGTTCAAGAGTCAGGAGATTCATGCGCATACTGGGTCGATTTGGAGTATCAAGTTTAGTTTGGATGGAAAATACTTGGCCAGTGCCGGTGAGGATTGCGTGATTCATATTTGGCAGGTAATGCAGAGTGAAAGGAAGGGTGACCTATTGATGGAGAAATCAGAAGATAGCAATTTAACCATGTTGTTATTTGCCAATGGATCACCGGAACCATGTTCAGTCTCGCCGAATGTGGACAACCATGTGGAAAAAAAGAGGAGAGGGAGGTCGTCTATCAGTCGAAAATCTGTTAGTTTGGACCATTATGTGATTCCAGAGACTGTGTTTGCACTTTCAGAGAAACCCATTTCTTCATTCGAAGGCCATCTGGATGATGTGCTTGACCTCTCTTGGTCCAAGTCGCAGCACCTGCTCTCATCTTCAATGGACAAAACCGTGCGCCTCTGGCACTTGTCTAGCAAGACTTGCCTGAAGATATTTTCACATAGTGACTATGTAACTTGCATCCAGTTCAATCCTGTTGATGATAGGTACTTCATTAGTGGGTCCCTTGATGCCAAGGTTCGCATATGGAGTATCCCTGATCATCAAGTGGTTGATTGGAATGATCTGCATGAGATGGTCACTGCTGCTTGCTACACTCCTGATGGTCAGGGCGCCCTAGTTGGTTCATACAAAGGCAGCTGCCGTTTATACAACACATCTGAGAACAAGTTGCAACAGAAAAGCCAAATTAATTTGCAGAACAAGAAAAAGAAATCCCATCACAAGAAAATTACAGGTTTTCAGTTTGCACCAGGAAGTTCATCAGAAGTATTGATTACATCTGCAGATTCACGAATCAGAGTTGTTGACGGTGTTGATCTGGTTCACAAGTTCAAGGGATTTCGGAACGCAAATAGCCAAATCTCAGCTGCCCTCACAGCAAATGGTAAATATGTGGTATCTGCTAGTGAGGATTCTCATGTATATATATGGAAACATGAAGCTGATTCCCGCCCTAGCCGAAGCAAGAGTGTCACAGTCACACGCTCCTATGAGCATTTCCATTGTCAAGATGTATCGGTCGCCATCCCCTGGCCTGGAATGGCTGATTTATGGGGTTTACAAGATTCAGAACGGAATGGTCTTGACGAGGTTTCCACAGCAAATCACCCCCCTACACCTGTTGAGGCGGCTAATTCCAACAACGAGGGTTCACGATCGGCATCTGGTTGCACCAATAGCCCTCTCCACGGAACAATTTCAAGTGCATCCAACAGCTACTTCTTTGATAGAATTTCGGCAACATGGCCGGAGGAAAAACTTCTTCAAGCTACCAGGAACCGAAGCCCGCGTGTTAGTTTTGATTTCACCAACGGGTTAAGCCAAAACATGTCAGCCTGGGGTATGGTGATTGTAACTGCCGGCCTTCGAGGTGAAATTAGAACTTTCCTAAATTTTGGGTTGCCGATTCGGATCTAA

Protein Analysis

878

Amino Acids

96.31

Weight (kDa)

6.3

Isoelectric Point (pI)

50.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 393 - 431 2.4e-07 THOC3 beta-propeller domain
WD40 PF00400 395 - 428 1.1e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 397 - 436 2.3e-06 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 485 - 566 2.5e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 500 - 719 9.7e-37 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 502 - 583 1.1e-12 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 502 - 628 1.1e-08 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 502 - 630 4e-15 Prp19 WD40 domain
EIF3I PF24805 503 - 705 1.3e-09 EIF3I
WD40 PF00400 504 - 539 2.1e-07 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 505 - 599 6.2e-09 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 506 - 549 9.3e-06 THOC3 beta-propeller domain
Beta-prop_IFT122_1st PF23381 508 - 625 2.3e-06 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 509 - 589 1.5e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 513 - 718 7.8e-24 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 515 - 633 3e-09 Aladin seven-bladed propeller
Beta-prop_WDR90_POC16_2nd PF23393 524 - 630 1.1e-08 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 525 - 716 5.7e-09 G protein beta WD-40 repeat protein
WD40 PF00400 545 - 582 8.4e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 553 - 724 3.3e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 567 - 729 1.1e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 587 - 718 2.4e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15470 AT5G54200
fragaria_vesca FvH4_3g20610 FvH4_3g20610
malus_domestica MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis RchiOBHm_Chr5g0034631
rosa_laevigata RLG00000033560
rosa_roxburghii Rroxscaffold_1G00045690
rosa_rugosa Rorug05G0147300
rosa_samantha Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana Rw5G021930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 540
AarI CACCTGC 1 cut(s) 1587
Acc16I TGCGCA 1 cut(s) 1197
Acc36I ACCTGC 2 cut(s) 1276, 1587
AccB1I GGYRCC 2 cut(s) 161, 1826
AccB7I CCANNNNNTGG 1 cut(s) 2247
AccBSI CCGCTC 1 cut(s) 378
AccI GTMKAC 1 cut(s) 615
AccII CGCG 2 cut(s) 995, 2506
AciI CCGC 9 cut(s) 307, 376, 572, 575, 587, 1024, 2167, 2345, 2504
AclWI GGATC 1 cut(s) 1378
AcoI YGGCCR 2 cut(s) 1248, 2462
AcsI RAATTY 4 cut(s) 149, 343, 2448, 2608
AcuI CTGAAG 1 cut(s) 1661
AcyI GRCGYC 2 cut(s) 162, 1827
AdeI CACNNNGTG 1 cut(s) 1658
AfaI GTAC 5 cut(s) 144, 176, 715, 802, 1703
AflII CTTAAG 1 cut(s) 788
AflIII ACRYGT 1 cut(s) 2544
AgeI ACCGGT 1 cut(s) 500
AjnI CCWGG 6 cut(s) 1095, 1966, 2246, 2251, 2489, 2552
AjuI GAANNNNNNNTTGG 2 cut(s) 2420, 2452
AloI GAACNNNNNNTCC 2 cut(s) 89, 121
AluBI AGCT 7 cut(s) 787, 1077, 1854, 2084, 2159, 2433, 2486
AluI AGCT 7 cut(s) 787, 1077, 1854, 2084, 2159, 2433, 2486
Alw21I GWGCWC 1 cut(s) 1079
Alw26I GTCTC 6 cut(s) 73, 248, 319, 908, 1396, 1481
AlwI GGATC 1 cut(s) 1378
AlwNI CAGNNNCTG 2 cut(s) 1490, 1579
Ama87I CYCGRG 2 cut(s) 267, 604
AoxI GGCC 8 cut(s) 111, 270, 1115, 1248, 1531, 2249, 2462, 2578
ApeKI GCWGC 6 cut(s) 393, 1573, 1799, 1851, 1854, 2084
ApoI RAATTY 4 cut(s) 149, 343, 2448, 2608
AseI ATTAAT 1 cut(s) 1905
AsiGI ACCGGT 1 cut(s) 500
Asp700I GAANNNNTTC 2 cut(s) 2413, 2475
AspLEI GCGC 4 cut(s) 164, 1198, 1611, 1829
AspS9I GGNCC 7 cut(s) 186, 259, 270, 1115, 1468, 1563, 1716
AsuC2I CCSGG 3 cut(s) 257, 268, 269
AsuII TTCGAA 3 cut(s) 635, 947, 1527
AvaI CYCGRG 2 cut(s) 267, 604
AvaII GGWCC 5 cut(s) 186, 259, 1468, 1563, 1716
BalI TGGCCA 1 cut(s) 1250
BanI GGYRCC 2 cut(s) 161, 1826
BanII GRGCYC 1 cut(s) 1079
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 1079
BbvI GCAGC 6 cut(s) 405, 1585, 1786, 1841, 1863, 2071
BceAI ACGGC 3 cut(s) 125, 406, 1842
BcgI CGANNNNNNTGC 2 cut(s) 902, 936
BciT130I CCWGG 6 cut(s) 1097, 1968, 2248, 2253, 2491, 2554
BciVI GTATCC 1 cut(s) 1757
BclI TGATCA 1 cut(s) 1753
BcnI CCSGG 3 cut(s) 257, 268, 269
BcoDI GTCTC 6 cut(s) 73, 248, 319, 908, 1396, 1481
BfaI CTAG 4 cut(s) 1626, 1832, 2121, 2172
BfmI CTRYAG 1 cut(s) 1998
BfoI RGCGCY 2 cut(s) 165, 1830
BfrI CTTAAG 1 cut(s) 788
BfuAI ACCTGC 2 cut(s) 1276, 1587
BfuI GTATCC 1 cut(s) 1757
BisI GCNGC 9 cut(s) 394, 575, 587, 1574, 1800, 1852, 1855, 2085, 2346
BlsI GCNGC 9 cut(s) 395, 576, 588, 1575, 1801, 1853, 1856, 2086, 2347
Bme1390I CCNGG 9 cut(s) 257, 268, 269, 1097, 1968, 2248, 2253, 2491, 2554
Bme18I GGWCC 5 cut(s) 186, 259, 1468, 1563, 1716
BmeT110I CYCGRG 2 cut(s) 267, 604
BmgT120I GGNCC 7 cut(s) 186, 259, 270, 1115, 1468, 1563, 1716
BmiI GGNNCC 8 cut(s) 163, 725, 910, 1380, 1717, 1718, 1828, 2495
BmrFI CCNGG 9 cut(s) 257, 268, 269, 1097, 1968, 2248, 2253, 2491, 2554
BmrI ACTGGG 2 cut(s) 293, 1212
BmsI GCATC 7 cut(s) 679, 691, 990, 1683, 1714, 2387, 2432
BmuI ACTGGG 2 cut(s) 293, 1212
BpiI GAAGAC 1 cut(s) 30
BplI GAGNNNNNCTC 2 cut(s) 185, 217
Bpu14I TTCGAA 3 cut(s) 635, 947, 1527
BpuEI CTTGAG 1 cut(s) 321
BpuMI CCSGG 3 cut(s) 257, 268, 269
BsaBI GATNNNNATC 2 cut(s) 2377, 2627
BsaHI GRCGYC 2 cut(s) 162, 1827
BsaI GGTCTC 3 cut(s) 248, 319, 908
BsaJI CCNNGG 7 cut(s) 108, 267, 819, 1728, 2246, 2404, 2553
BsaWI WCCGGW 2 cut(s) 500, 1375
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bse118I RCCGGY 6 cut(s) 216, 248, 293, 500, 1256, 2576
Bse1I ACTGG 5 cut(s) 288, 808, 1207, 1251, 1678
Bse3DI GCAATG 1 cut(s) 667
Bse8I GATNNNNATC 2 cut(s) 2377, 2627
BseBI CCWGG 6 cut(s) 1097, 1968, 2248, 2253, 2491, 2554
BseDI CCNNGG 7 cut(s) 108, 267, 819, 1728, 2246, 2404, 2553
BseGI GGATG 7 cut(s) 910, 1105, 1240, 1546, 1674, 2241, 2423
BseJI GATNNNNATC 2 cut(s) 2377, 2627
BseMI GCAATG 1 cut(s) 667
BseMII CTCAG 2 cut(s) 1866, 2094
BseNI ACTGG 5 cut(s) 288, 808, 1207, 1251, 1678
BseRI GAGGAG 9 cut(s) 61, 64, 218, 622, 659, 662, 839, 889, 1441
BseXI GCAGC 6 cut(s) 405, 1585, 1786, 1841, 1863, 2071
Bsh1236I CGCG 2 cut(s) 995, 2506
Bsh1285I CGRYCG 4 cut(s) 61, 311, 2238, 2375
BshFI GGCC 8 cut(s) 113, 272, 1117, 1250, 1533, 2251, 2464, 2580
BshNI GGYRCC 2 cut(s) 161, 1826
BshTI ACCGGT 1 cut(s) 500
BsiEI CGRYCG 4 cut(s) 61, 311, 2238, 2375
BsiHKAI GWGCWC 1 cut(s) 1079
BsiHKCI CYCGRG 2 cut(s) 267, 604
BslFI GGGAC 3 cut(s) 37, 1055, 1702
BsmAI GTCTC 6 cut(s) 73, 248, 319, 908, 1396, 1481
BsmFI GGGAC 3 cut(s) 37, 1055, 1702
BsmI GAATGC 1 cut(s) 847
BsnI GGCC 8 cut(s) 113, 272, 1117, 1250, 1533, 2251, 2464, 2580
Bso31I GGTCTC 3 cut(s) 248, 319, 908
BsoBI CYCGRG 2 cut(s) 267, 604
Bsp119I TTCGAA 3 cut(s) 635, 947, 1527
Bsp1286I GDGCHC 1 cut(s) 1079
Bsp19I CCATGG 1 cut(s) 819
Bsp68I TCGCGA 1 cut(s) 995
BspACI CCGC 9 cut(s) 307, 376, 572, 575, 587, 1024, 2167, 2345, 2504
BspANI GGCC 8 cut(s) 113, 272, 1117, 1250, 1533, 2251, 2464, 2580
BspCNI CTCAG 2 cut(s) 1867, 2093
BspFNI CGCG 2 cut(s) 995, 2506
BspLI GGNNCC 8 cut(s) 163, 725, 910, 1380, 1717, 1718, 1828, 2495
BspMAI CTGCAG 1 cut(s) 2002
BspMI ACCTGC 2 cut(s) 1276, 1587
BspPI GGATC 1 cut(s) 1378
BspT104I TTCGAA 3 cut(s) 635, 947, 1527
BspT107I GGYRCC 2 cut(s) 161, 1826
BspTI CTTAAG 1 cut(s) 788
BspTNI GGTCTC 3 cut(s) 248, 319, 908
BsrBI CCGCTC 1 cut(s) 378
BsrDI GCAATG 1 cut(s) 667
BsrFI RCCGGY 6 cut(s) 216, 248, 293, 500, 1256, 2576
BsrI ACTGG 5 cut(s) 288, 808, 1207, 1251, 1678
BssAI RCCGGY 6 cut(s) 216, 248, 293, 500, 1256, 2576
BssECI CCNNGG 7 cut(s) 108, 267, 819, 1728, 2246, 2404, 2553
BssNI GRCGYC 2 cut(s) 162, 1827
BssT1I CCWWGG 2 cut(s) 819, 1728
Bst2UI CCWGG 6 cut(s) 1097, 1968, 2248, 2253, 2491, 2554
Bst4CI ACNGT 5 cut(s) 353, 1492, 1606, 2027, 2194
Bst6I CTCTTC 1 cut(s) 885
BstACI GRCGYC 2 cut(s) 162, 1827
BstAFI CTTAAG 1 cut(s) 788
BstAPI GCANNNNNTGC 3 cut(s) 1291, 1579, 1635
BstBI TTCGAA 3 cut(s) 635, 947, 1527
BstC8I GCNNGC 4 cut(s) 599, 1804, 2504, 2578
BstDEI CTNAG 2 cut(s) 1875, 2080
BstDSI CCRYGG 2 cut(s) 819, 2404
BstEII GGTNACC 1 cut(s) 1310
BstF5I GGATG 7 cut(s) 910, 1105, 1240, 1546, 1674, 2241, 2423
BstFNI CGCG 2 cut(s) 995, 2506
BstH2I RGCGCY 2 cut(s) 165, 1830
BstHHI GCGC 4 cut(s) 164, 1198, 1611, 1829
BstMAI GTCTC 6 cut(s) 73, 248, 319, 908, 1396, 1481
BstMCI CGRYCG 4 cut(s) 61, 311, 2238, 2375
BstMWI GCNNNNNNNGC 9 cut(s) 366, 375, 583, 1291, 1579, 1615, 1635, 2093, 2394
BstNI CCWGG 6 cut(s) 1097, 1968, 2248, 2253, 2491, 2554
BstNSI RCATGY 1 cut(s) 2548
BstPI GGTNACC 1 cut(s) 1310
BstSCI CCNGG 9 cut(s) 255, 266, 267, 1095, 1966, 2246, 2251, 2489, 2552
BstSFI CTRYAG 1 cut(s) 1998
BstUI CGCG 2 cut(s) 995, 2506
BstV1I GCAGC 6 cut(s) 405, 1585, 1786, 1841, 1863, 2071
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 2628
BstYI RGATCY 1 cut(s) 2628
BsuI GTATCC 1 cut(s) 1757
BsuRI GGCC 8 cut(s) 113, 272, 1117, 1250, 1533, 2251, 2464, 2580
BtgI CCRYGG 2 cut(s) 819, 2404
BtgZI GCGATG 1 cut(s) 1047
BtsCI GGATG 7 cut(s) 910, 1105, 1240, 1546, 1674, 2241, 2423
BtsI GCAGTG 2 cut(s) 1164, 1794
BtsIMutI CAGTG 4 cut(s) 407, 1164, 1258, 1794
BtuMI TCGCGA 1 cut(s) 995
BveI ACCTGC 2 cut(s) 1276, 1587
Cac8I GCNNGC 4 cut(s) 599, 1804, 2504, 2578
CaiI CAGNNNCTG 2 cut(s) 1490, 1579
CfoI GCGC 4 cut(s) 164, 1198, 1611, 1829
Cfr10I RCCGGY 6 cut(s) 216, 248, 293, 500, 1256, 2576
Cfr13I GGNCC 7 cut(s) 186, 259, 270, 1115, 1468, 1563, 1716
Cfr9I CCCGGG 1 cut(s) 267
Csp6I GTAC 5 cut(s) 143, 175, 714, 801, 1702
CspAI ACCGGT 1 cut(s) 500
CviQI GTAC 5 cut(s) 143, 175, 714, 801, 1702
DdeI CTNAG 2 cut(s) 1875, 2080
DinI GGCGCC 2 cut(s) 163, 1828
DraIII CACNNNGTG 1 cut(s) 1658
EaeI YGGCCR 2 cut(s) 1248, 2462
Eam1104I CTCTTC 1 cut(s) 885
EarI CTCTTC 1 cut(s) 885
EciI GGCGGA 2 cut(s) 561, 1039
Ecl136II GAGCTC 1 cut(s) 1077
Eco130I CCWWGG 2 cut(s) 819, 1728
Eco24I GRGCYC 1 cut(s) 1079
Eco31I GGTCTC 3 cut(s) 248, 319, 908
Eco47I GGWCC 5 cut(s) 186, 259, 1468, 1563, 1716
Eco53kI GAGCTC 1 cut(s) 1077
Eco57I CTGAAG 1 cut(s) 1661
Eco88I CYCGRG 2 cut(s) 267, 604
Eco91I GGTNACC 1 cut(s) 1310
EcoICRI GAGCTC 1 cut(s) 1077
EcoO109I RGGNCCY 1 cut(s) 1716
EcoO65I GGTNACC 1 cut(s) 1310
EcoRI GAATTC 1 cut(s) 343
EcoRII CCWGG 6 cut(s) 1095, 1966, 2246, 2251, 2489, 2552
EcoT14I CCWWGG 2 cut(s) 819, 1728
EcoT38I GRGCYC 1 cut(s) 1079
EgeI GGCGCC 2 cut(s) 163, 1828
EheI GGCGCC 2 cut(s) 163, 1828
ErhI CCWWGG 2 cut(s) 819, 1728
FaqI GGGAC 3 cut(s) 37, 1055, 1702
FauI CCCGC 2 cut(s) 2174, 2511
FauNDI CATATG 1 cut(s) 1739
FbaI TGATCA 1 cut(s) 1753
FblI GTMKAC 1 cut(s) 615
Fnu4HI GCNGC 9 cut(s) 394, 575, 587, 1574, 1800, 1852, 1855, 2085, 2346
FokI GGATG 7 cut(s) 917, 1112, 1247, 1553, 1661, 2228, 2410
FriOI GRGCYC 1 cut(s) 1079
Fsp4HI GCNGC 9 cut(s) 394, 575, 587, 1574, 1800, 1852, 1855, 2085, 2346
FspAI RTGCGCAY 1 cut(s) 1197
FspBI CTAG 4 cut(s) 1626, 1832, 2121, 2172
FspI TGCGCA 1 cut(s) 1197
GlaI GCGC 4 cut(s) 163, 1197, 1610, 1828
GluI GCNGC 9 cut(s) 394, 575, 587, 1574, 1800, 1852, 1855, 2085, 2346
HaeII RGCGCY 2 cut(s) 165, 1830
HaeIII GGCC 8 cut(s) 113, 272, 1117, 1250, 1533, 2251, 2464, 2580
HhaI GCGC 4 cut(s) 164, 1198, 1611, 1829
Hin1I GRCGYC 2 cut(s) 162, 1827
Hin6I GCGC 4 cut(s) 162, 1196, 1609, 1827
HinP1I GCGC 4 cut(s) 162, 1196, 1609, 1827
HincII GTYRAC 2 cut(s) 816, 2023
HindII GTYRAC 2 cut(s) 816, 2023
HindIII AAGCTT 1 cut(s) 785
Hpy166II GTNNAC 8 cut(s) 616, 714, 816, 1405, 2023, 2041, 2276, 2369
Hpy8I GTNNAC 8 cut(s) 616, 714, 816, 1405, 2023, 2041, 2276, 2369
Hpy99I CGWCG 4 cut(s) 182, 322, 353, 392
HpyAV CCTTC 6 cut(s) 207, 600, 631, 1300, 1523, 2591
HpyCH4III ACNGT 5 cut(s) 353, 1492, 1606, 2027, 2194
HpyCH4IV ACGT 2 cut(s) 180, 776
HpyF10VI GCNNNNNNNGC 9 cut(s) 366, 375, 583, 1291, 1579, 1615, 1635, 2093, 2394
HpyF3I CTNAG 2 cut(s) 1875, 2080
HpySE526I ACGT 2 cut(s) 180, 776
Hsp92I GRCGYC 2 cut(s) 162, 1827
HspAI GCGC 4 cut(s) 162, 1196, 1609, 1827
KasI GGCGCC 2 cut(s) 161, 1826
KflI GGGWCCC 1 cut(s) 1716
KroI GCCGGC 1 cut(s) 2576
KroNI GCCGGC 1 cut(s) 2578
Ksp22I TGATCA 1 cut(s) 1753
LmnI GCTCC 2 cut(s) 383, 2207
Lsp1109I GCAGC 6 cut(s) 405, 1585, 1786, 1841, 1863, 2071
LweI GCATC 7 cut(s) 679, 691, 990, 1683, 1714, 2387, 2432
MaeI CTAG 4 cut(s) 1626, 1832, 2121, 2172
MaeII ACGT 2 cut(s) 180, 776
MaeIII GTNAC 8 cut(s) 1162, 1310, 1658, 1666, 1792, 2188, 2194, 2569
MbiI CCGCTC 1 cut(s) 378
MflI RGATCY 1 cut(s) 2628
MhlI GDGCHC 1 cut(s) 1079
MlsI TGGCCA 1 cut(s) 1250
MluNI TGGCCA 1 cut(s) 1250
Mly113I GGCGCC 2 cut(s) 162, 1827
MlyI GAGTC 3 cut(s) 59, 98, 1189
MmeI TCCRAC 8 cut(s) 126, 300, 372, 411, 633, 1046, 2379, 2451
Mox20I TGGCCA 1 cut(s) 1250
MroNI GCCGGC 1 cut(s) 2576
MroXI GAANNNNTTC 2 cut(s) 2413, 2475
MscI TGGCCA 1 cut(s) 1250
MseI TTAA 7 cut(s) 363, 672, 789, 974, 1346, 1905, 2534
MslI CAYNNNNRTG 3 cut(s) 236, 903, 1539
Msp20I TGGCCA 1 cut(s) 1250
MspA1I CMGCKG 2 cut(s) 1854, 2084
MspCI CTTAAG 1 cut(s) 788
MspR9I CCNGG 9 cut(s) 257, 268, 269, 1097, 1968, 2248, 2253, 2491, 2554
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 6 cut(s) 1097, 1968, 2248, 2253, 2491, 2554
MvnI CGCG 2 cut(s) 995, 2506
MwoI GCNNNNNNNGC 9 cut(s) 366, 375, 583, 1291, 1579, 1615, 1635, 2093, 2394
NaeI GCCGGC 1 cut(s) 2578
NarI GGCGCC 2 cut(s) 162, 1827
NciI CCSGG 3 cut(s) 257, 268, 269
NcoI CCATGG 1 cut(s) 819
NdeI CATATG 1 cut(s) 1739
NgoMIV GCCGGC 1 cut(s) 2576
NlaIV GGNNCC 8 cut(s) 163, 725, 910, 1380, 1717, 1718, 1828, 2495
NmuCI GTSAC 6 cut(s) 1162, 1310, 1658, 1792, 2188, 2194
NruI TCGCGA 1 cut(s) 995
NsbI TGCGCA 1 cut(s) 1197
NspI RCATGY 1 cut(s) 2548
NspV TTCGAA 3 cut(s) 635, 947, 1527
PaeR7I CTCGAG 1 cut(s) 604
PaqCI CACCTGC 1 cut(s) 1587
PciI ACATGT 1 cut(s) 2544
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 357
PctI GAATGC 1 cut(s) 847
PdiI GCCGGC 1 cut(s) 2578
PdmI GAANNNNTTC 2 cut(s) 2413, 2475
PflFI GACNNNGTC 1 cut(s) 257
PflMI CCANNNNNTGG 1 cut(s) 2247
PinAI ACCGGT 1 cut(s) 500
PkrI GCNGC 9 cut(s) 395, 576, 588, 1575, 1801, 1853, 1856, 2086, 2347
Ple19I CGATCG 2 cut(s) 61, 2375
PleI GAGTC 3 cut(s) 58, 98, 1188
PluTI GGCGCC 2 cut(s) 165, 1830
PpsI GAGTC 3 cut(s) 58, 98, 1188
PpuMI RGGWCCY 1 cut(s) 1716
PscI ACATGT 1 cut(s) 2544
PshBI ATTAAT 1 cut(s) 1905
PsiI TTATAA 1 cut(s) 540
Psp124BI GAGCTC 1 cut(s) 1079
Psp5II RGGWCCY 1 cut(s) 1716
Psp6I CCWGG 6 cut(s) 1095, 1966, 2246, 2251, 2489, 2552
PspEI GGTNACC 1 cut(s) 1310
PspGI CCWGG 6 cut(s) 1095, 1966, 2246, 2251, 2489, 2552
PspN4I GGNNCC 8 cut(s) 163, 725, 910, 1380, 1717, 1718, 1828, 2495
PspPI GGNCC 7 cut(s) 186, 259, 270, 1115, 1468, 1563, 1716
PspPPI RGGWCCY 1 cut(s) 1716
PspXI VCTCGAGB 1 cut(s) 604
PstI CTGCAG 1 cut(s) 2002
PstNI CAGNNNCTG 2 cut(s) 1490, 1579
PsuI RGATCY 1 cut(s) 2628
PsyI GACNNNGTC 1 cut(s) 257
PvuI CGATCG 2 cut(s) 61, 2375
PvuII CAGCTG 2 cut(s) 1854, 2084
RruI TCGCGA 1 cut(s) 995
RsaI GTAC 5 cut(s) 144, 176, 715, 802, 1703
RsaNI GTAC 5 cut(s) 143, 175, 714, 801, 1702
RseI CAYNNNNRTG 3 cut(s) 236, 903, 1539
SacI GAGCTC 1 cut(s) 1079
SaqAI TTAA 7 cut(s) 363, 672, 789, 974, 1346, 1905, 2534
SatI GCNGC 9 cut(s) 394, 575, 587, 1574, 1800, 1852, 1855, 2085, 2346
Sau96I GGNCC 7 cut(s) 186, 259, 270, 1115, 1468, 1563, 1716
SchI GAGTC 3 cut(s) 59, 98, 1189
ScrFI CCNGG 9 cut(s) 257, 268, 269, 1097, 1968, 2248, 2253, 2491, 2554
SduI GDGCHC 1 cut(s) 1079
SfaNI GCATC 7 cut(s) 679, 691, 990, 1683, 1714, 2387, 2432
SfcI CTRYAG 1 cut(s) 1998
SfoI GGCGCC 2 cut(s) 163, 1828
Sfr274I CTCGAG 1 cut(s) 604
SfuI TTCGAA 3 cut(s) 635, 947, 1527
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 5 cut(s) 186, 259, 1468, 1563, 1716
SlaI CTCGAG 1 cut(s) 604
SmaI CCCGGG 1 cut(s) 269
SmiMI CAYNNNNRTG 3 cut(s) 236, 903, 1539
SmlI CTYRAG 3 cut(s) 336, 604, 788
SmoI CTYRAG 3 cut(s) 336, 604, 788
SsiI CCGC 9 cut(s) 307, 376, 572, 575, 587, 1024, 2167, 2345, 2504
SspDI GGCGCC 2 cut(s) 161, 1826
SspMI CTAG 4 cut(s) 1626, 1832, 2121, 2172
SstI GAGCTC 1 cut(s) 1079
StyD4I CCNGG 9 cut(s) 255, 266, 267, 1095, 1966, 2246, 2251, 2489, 2552
StyI CCWWGG 2 cut(s) 819, 1728
TaaI ACNGT 5 cut(s) 353, 1492, 1606, 2027, 2194
TaiI ACGT 2 cut(s) 183, 779
TaqII GACCGA 3 cut(s) 301, 497, 2224
TatI WGTACW 1 cut(s) 713
TauI GCSGC 3 cut(s) 577, 589, 2348
Tru1I TTAA 7 cut(s) 363, 672, 789, 974, 1346, 1905, 2534
Tru9I TTAA 7 cut(s) 363, 672, 789, 974, 1346, 1905, 2534
TscAI CASTG 4 cut(s) 407, 1171, 1258, 1801
TseFI GTSAC 6 cut(s) 1162, 1310, 1658, 1792, 2188, 2194
TseI GCWGC 6 cut(s) 393, 1573, 1799, 1851, 1854, 2084
Tsp45I GTSAC 6 cut(s) 1162, 1310, 1658, 1792, 2188, 2194
TspGWI ACGGA 2 cut(s) 2305, 2421
TspMI CCCGGG 1 cut(s) 267
TspRI CASTG 4 cut(s) 407, 1171, 1258, 1801
Tth111I GACNNNGTC 1 cut(s) 257
Van91I CCANNNNNTGG 1 cut(s) 2247
Vha464I CTTAAG 1 cut(s) 788
VpaK11BI GGWCC 5 cut(s) 186, 259, 1468, 1563, 1716
VspI ATTAAT 1 cut(s) 1905
XapI RAATTY 4 cut(s) 149, 343, 2448, 2608
XceI RCATGY 1 cut(s) 2548
XcmI CCANNNNNNNNNTGG 2 cut(s) 154, 280
XhoI CTCGAG 1 cut(s) 604
XmaI CCCGGG 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 615
XmnI GAANNNNTTC 2 cut(s) 2413, 2475
XspI CTAG 4 cut(s) 1626, 1832, 2121, 2172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.