RchiOBHm_Chr5g0034631

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011080
Physical Location & Seq
Reverse (-)
28494189 .. 28499892
5704 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31360

Sequence Viewer

Length: 2625 bp
ATGAGCAAAGCCGGGGAGGAAGAAGACGAAGACGAGTGCTTCTACGAGTCCCTCGATCGCATTCTCGTCTCCTCCTCCTCCTCCTGTTCTTGCTCCACCTCCACCTCCACCTCCGACGACGACGACGACGACGCCGCCGTCTCTTCTTCTCCTCCCAATTATGCTTCCCGCAAGCGCTTTCCCACCCCAAAGTTCCCAATGGGGGCCTACAAGTACGACGTCTGGATCTCCGAGCCCGCCTCCGTCTCCGACCGCCGCTTACGTCTCCTCCGCGAAATGGGCCTCAGCGGCGACCCGGCCCTCTCGCGCGCCAAGCCCGACCCGGTGGGCCGCTCGGCCTCGTCGGATCATTTGACTAAACGACACAGCCCTGCCGCCGTTCCGGAAATCGTCCGGTCCAAATCGGACGGCGTCGAACACTGCAACGGCGCTGCATCCGCTAGTTCGTCGCCTATTCTTTGTTCCGAGATTGAGGCTCCGGCTCCGGCCGAGACCGAGACCGAGACCAAAAATTCGTTTGTAAATACACAGAAAAAAAAAGATTGCGTCGTTTGTAAATCGGCGAGCGGCAAGAGCAATGGACCTTCTCCGTCGCCGCCGCCGAATAGGCCGCCATCCGGGAAGAACGGCCGGCGGTGCGGCGACGAGACTGGGTTAGAGGTGGAGGAGGAGCAGGAGGAGGAGGAGGAGAGTGAAGTGTGTACTATTCGGAATCTTGATAATGGGAAGGAGTTTGTGGTGAATGAGATCAGGGAAGATGGGATGTGGAACAAGCTTAAGGAAGTGGGGACCGGGAAGCAGTTGACCATGGAGGAGTTTGAGATGAGTGTGGGCCATTCCCCCATTGTTCAGGAACTGATGCGGCGGCAGAATGTGGAGGAGAGTCACAAGGCCGGTGGTGGTTCGGATTCGAATGCCAATGGGGGCGGTGGAGCGATTCCCAAGTTGAAGAAGAGGGGTTGGTTCAAGAGCATAAAGAGTGTTGCGAGCACTGTGACTGGGCATAGGGAGAGAAGGAGCAGTGATGAAAGGGACACTTCGTCGGAGAAGGGGGGCCGGAGGTCAAGCTCTGCCACTGATGATAGCCAGGATGTGTCGTTTCATGGGCCGGAGAGAGTGAGGGTCAGGCAGTATGGCAAGTCTTGTAAGGAGCTGACGGCTATGTACAAGACACAGGAGATTCAGGCGCATAATGGGTCTATTTGGAGTATTAAGTTTAGCTTGGATGGGAAGTATCTGGCCAGTGCCGGTGAGGATTGTGTGATTCATATATGGCAGGTGATGGAGACGGAGAGGAAGGGTGACCTGTTGATGCAGAAATCGGAAGATAACAATTTGGATTTGTTGTTTATTGCCAATGGATCTTCAGAACCATGTTCCGTCTCGCCCAATAGTTACAGCCATCTGGAAAAAAAGAAGAGAGGGAGGACGTCTATCAGTCGGAAATCATTGAGTTTGGAACATTATGTGATTCCAGAGACTATGTTTGCACTCTCAGAAAAGCCCATCTGTTCATTCCAAGGTCATGTAGATGATGTGCTTGACCTCTCTTGGTCCAAGTCTCAGCGCCTGCTCTCATCTTCTATGGATAAAACGGTTCACCTCTGGGATTTGCCAAGCAAGTCTTGTTTGCAGAAGTTTTCACACATTGACTATGTAACTTGCATCCAGTTTAATCCCGTTGATGATAACTACTTCATTAGTGGATCACTTGATGCTAAGGTTCGTATATGGAGCATCACTGATCGCCAAGTGGTTGATTGGAATGATATGCATGAGATGGTCACTGCTGCTTGCTACACTCCTGATGGCCAGGGTGCTCTAGTTGGAACCTACAAAGGGAGCTGCCGGTTATACAACACATCTGATAATAAGTTGCAAGAGAAAAGCCAAATAAATCTGCAGAACAAGAAAAAGAAATCTCATCACAAGAAAATTACTGGTTTCCAGTTTGCACCAGGAAGTTCCTCGGAAGTACTAGTTACATCTGCAGATTCACGAGTCAGGGTTGTCGATGGTATTGATCTAGTTCACAAGTTCAAGGGATTTCGTAACACAAATAGCCAAATCTCAGCAACCCTCACAGTAAACGGTAAATATGTGGTATCTGCCAGCGAGGATTCTCATGTATATATATGGAAACATGAAGCTGATTCGCGCCCTAGCAGAAGCAAAAGTGTTACTGTTACACGGTCGTATGAGCATTTTCATAGTCACAATGTATCAGTGGCCGTCTCTTGGCCTGGTGTTGGTGATTCATGGGGACTACAAGATGGAGATCAAAACAATCTTGATGAGGTTTCTACAGCCAATCATCCCCCTACTCCTGTTGAGGAGGAGGCTAATGGCAAGGATGGTTCACGATCAGCATCGGGTTGCACCAATAGCCCTCTCCATGGAACAATTACTAGTGCATCCAACAGTTACTTCTTTGATAGAATTTCGGCAACGTGGCCTGAGGAAAAACTTCTTCTAGCTACTAAGAAACGAAGCCCTCGTGTAAGTTTTGATTTTAGCAATGGTGTAAGCCAGAACATTCCGGCCTGGGGTATGGTGATTGTAACCGCTGGCTTGGGAGGTGAAATTAGAACTTTCCAAAATTTTGGGTTGCCGATTCGGATTTAA

Protein Analysis

874

Amino Acids

96.21

Weight (kDa)

6.13

Isoelectric Point (pI)

55.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 386 - 429 6.1e-08 THOC3 beta-propeller domain
WD40 PF00400 391 - 425 1.2e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 394 - 434 1.6e-06 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 499 - 580 1.4e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 500 - 626 3.8e-14 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 501 - 717 1.8e-34 WDR5 beta-propeller domain
WD40 PF00400 501 - 537 2.2e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 501 - 563 8.2e-08 WDR36/Utp21 second beta-propeller domain
EIF3I PF24805 502 - 702 3.9e-09 EIF3I
WD40_CDC20-Fz PF24807 502 - 586 4e-10 CDC20/Fizzy WD40 domain
WDR55 PF24796 503 - 631 8.9e-07 WDR55
Beta-prop_THOC3 PF25174 505 - 548 2.9e-06 THOC3 beta-propeller domain
Beta-prop_IFT122_1st PF23381 506 - 622 3.1e-07 IFT122 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 506 - 586 1.1e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 508 - 630 3.4e-09 Aladin seven-bladed propeller
Beta-prop_THOC3 PF25174 509 - 715 1.9e-22 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 512 - 713 1e-11 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 521 - 627 3.2e-08 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 523 - 713 2.7e-08 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 552 - 721 2.3e-11 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 563 - 725 2.1e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 583 - 715 7.6e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G15470 AT5G54200
fragaria_vesca FvH4_3g20610 FvH4_3g20610
malus_domestica MD03G1223700.v1.1 MD11G1241600.v1.1
prunus_persica Prupe.4G185800_v2.0.a1 Prupe.4G185800_v2.0.a1
pyrus_communis pycom03g17200 pycom03g17210 pycom11g21320
rosa_chinensis RchiOBHm_Chr5g0034631
rosa_laevigata RLG00000033560
rosa_roxburghii Rroxscaffold_1G00045690
rosa_rugosa Rorug05G0147300
rosa_samantha Rh5AG239300 Rh5BG240000 Rh5CG270400 Rh5DG248200
rosa_wichuraiana Rw5G021930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1267
AasI GACNNNNNNGTC 1 cut(s) 137
AatII GACGTC 2 cut(s) 222, 1433
Acc36I ACCTGC 1 cut(s) 1267
AccBSI CCGCTC 2 cut(s) 333, 567
AccII CGCG 4 cut(s) 273, 307, 309, 2158
AccIII TCCGGA 1 cut(s) 382
AclWI GGATC 4 cut(s) 233, 354, 1369, 1714
AcoI YGGCCR 5 cut(s) 486, 628, 1239, 1810, 2229
AcsI RAATTY 3 cut(s) 511, 2439, 2599
AcuI CTGAAG 1 cut(s) 1350
AcyI GRCGYC 4 cut(s) 132, 219, 411, 1430
AfaI GTAC 4 cut(s) 215, 703, 1166, 1977
AfeI AGCGCT 1 cut(s) 176
AfiI CCNNNNNNNGG 8 cut(s) 202, 277, 617, 1839, 2238, 2249, 2396, 2546
AflII CTTAAG 1 cut(s) 776
AgsI TTSAA 3 cut(s) 949, 967, 2041
AhdI GACNNNNNGTC 1 cut(s) 1039
AhlI ACTAGT 2 cut(s) 1978, 2408
AjnI CCWGG 5 cut(s) 1086, 1812, 1957, 2242, 2543
AjuI GAANNNNNNNTTGG 2 cut(s) 2411, 2443
AluBI AGCT 7 cut(s) 775, 1068, 1153, 1221, 1845, 2150, 2477
AluI AGCT 7 cut(s) 775, 1068, 1153, 1221, 1845, 2150, 2477
Alw21I GWGCWC 2 cut(s) 992, 1822
AlwI GGATC 4 cut(s) 233, 354, 1369, 1714
AlwNI CAGNNNCTG 2 cut(s) 856, 1570
Aor13HI TCCGGA 1 cut(s) 382
Aor51HI AGCGCT 1 cut(s) 176
ApeKI GCWGC 3 cut(s) 431, 1790, 1845
ApoI RAATTY 3 cut(s) 511, 2439, 2599
ArsI GACNNNNNNTTYG 2 cut(s) 496, 528
Asp700I GAANNNNTTC 1 cut(s) 2466
AspLEI GCGC 7 cut(s) 177, 309, 311, 431, 1189, 1569, 2160
AsuC2I CCSGG 5 cut(s) 13, 296, 323, 619, 793
AsuHPI GGTGA 8 cut(s) 751, 1262, 1291, 1313, 1592, 2264, 2566, 2591
AsuII TTCGAA 1 cut(s) 911
AvaII GGWCC 4 cut(s) 396, 581, 789, 1554
AxyI CCTNAGG 1 cut(s) 2457
BalI TGGCCA 2 cut(s) 1241, 1812
BanII GRGCYC 1 cut(s) 237
BauI CACGAG 2 cut(s) 1998, 2496
BbsI GAAGAC 2 cut(s) 30, 36
Bbv12I GWGCWC 2 cut(s) 992, 1822
BbvCI CCTCAGC 1 cut(s) 284
BbvI GCAGC 3 cut(s) 418, 1777, 1832
BceAI ACGGC 7 cut(s) 122, 362, 424, 442, 643, 1173, 2216
BciT130I CCWGG 5 cut(s) 1088, 1814, 1959, 2244, 2545
BcnI CCSGG 5 cut(s) 13, 296, 323, 619, 793
BcuI ACTAGT 2 cut(s) 1978, 2408
BfaI CTAG 7 cut(s) 441, 1823, 1979, 2027, 2163, 2409, 2474
BfmI CTRYAG 3 cut(s) 1901, 1989, 2304
BfoI RGCGCY 3 cut(s) 178, 432, 1570
BfrI CTTAAG 1 cut(s) 776
BfuAI ACCTGC 1 cut(s) 1267
BglI GCCNNNNNGGC 2 cut(s) 288, 607
BmcAI AGTACT 1 cut(s) 1977
Bme18I GGWCC 4 cut(s) 396, 581, 789, 1554
BmeRI GACNNNNNGTC 1 cut(s) 1039
BmiI GGNNCC 6 cut(s) 205, 477, 483, 790, 1055, 1831
BmrI ACTGGG 2 cut(s) 660, 1008
BmsI GCATC 8 cut(s) 443, 849, 1302, 1674, 1705, 1746, 2378, 2423
BmuI ACTGGG 2 cut(s) 660, 1008
BpiI GAAGAC 2 cut(s) 30, 36
BplI GAGNNNNNCTC 2 cut(s) 224, 256
Bpu10I CCTNAGC 2 cut(s) 284, 1719
Bpu14I TTCGAA 1 cut(s) 911
BpuMI CCSGG 5 cut(s) 13, 296, 323, 619, 793
BsaBI GATNNNNATC 2 cut(s) 2277, 2368
BsaHI GRCGYC 4 cut(s) 132, 219, 411, 1430
BsaI GGTCTC 3 cut(s) 485, 491, 497
BsaJI CCNNGG 7 cut(s) 12, 807, 1519, 1813, 1968, 2395, 2544
BsaWI WCCGGW 2 cut(s) 382, 393
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 8 cut(s) 202, 277, 617, 1839, 2238, 2249, 2396, 2546
Bse118I RCCGGY 4 cut(s) 630, 893, 1247, 1848
Bse1I ACTGG 6 cut(s) 655, 1003, 1242, 1669, 1945, 1948
Bse21I CCTNAGG 1 cut(s) 2457
Bse3DI GCAATG 2 cut(s) 583, 2524
Bse8I GATNNNNATC 2 cut(s) 2277, 2368
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 5 cut(s) 1088, 1814, 1959, 2244, 2545
BseDI CCNNGG 7 cut(s) 12, 807, 1519, 1813, 1968, 2395, 2544
BseGI GGATG 9 cut(s) 434, 614, 768, 1096, 1231, 1665, 2314, 2359, 2414
BseJI GATNNNNATC 2 cut(s) 2277, 2368
BseLI CCNNNNNNNGG 8 cut(s) 202, 277, 617, 1839, 2238, 2249, 2396, 2546
BseMI GCAATG 2 cut(s) 583, 2524
BseMII CTCAG 5 cut(s) 298, 1509, 1577, 2085, 2448
BseNI ACTGG 6 cut(s) 655, 1003, 1242, 1669, 1945, 1948
BsePI GCGCGC 1 cut(s) 307
BseX3I CGGCCG 2 cut(s) 486, 628
BseXI GCAGC 3 cut(s) 418, 1777, 1832
Bsh1236I CGCG 4 cut(s) 273, 307, 309, 2158
Bsh1285I CGRYCG 5 cut(s) 58, 253, 489, 631, 2195
BsiEI CGRYCG 5 cut(s) 58, 253, 489, 631, 2195
BsiHKAI GWGCWC 2 cut(s) 992, 1822
BslFI GGGAC 4 cut(s) 34, 802, 1046, 2277
BslI CCNNNNNNNGG 8 cut(s) 202, 277, 617, 1839, 2238, 2249, 2396, 2546
BsmBI CGTCTC 7 cut(s) 73, 145, 250, 269, 1280, 1387, 2239
BsmFI GGGAC 4 cut(s) 34, 802, 1046, 2277
BsmI GAATGC 2 cut(s) 60, 919
Bso31I GGTCTC 3 cut(s) 485, 491, 497
Bsp119I TTCGAA 1 cut(s) 911
Bsp1286I GDGCHC 3 cut(s) 237, 992, 1822
Bsp13I TCCGGA 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 1164
Bsp19I CCATGG 2 cut(s) 807, 2395
BspCNI CTCAG 5 cut(s) 297, 1508, 1576, 2084, 2449
BspEI TCCGGA 1 cut(s) 382
BspFNI CGCG 4 cut(s) 273, 307, 309, 2158
BspLI GGNNCC 6 cut(s) 205, 477, 483, 790, 1055, 1831
BspMAI CTGCAG 2 cut(s) 1905, 1993
BspMI ACCTGC 1 cut(s) 1267
BspPI GGATC 4 cut(s) 233, 354, 1369, 1714
BspT104I TTCGAA 1 cut(s) 911
BspTI CTTAAG 1 cut(s) 776
BspTNI GGTCTC 3 cut(s) 485, 491, 497
BsrBI CCGCTC 2 cut(s) 333, 567
BsrDI GCAATG 2 cut(s) 583, 2524
BsrFI RCCGGY 4 cut(s) 630, 893, 1247, 1848
BsrGI TGTACA 1 cut(s) 1164
BsrI ACTGG 6 cut(s) 655, 1003, 1242, 1669, 1945, 1948
BssAI RCCGGY 4 cut(s) 630, 893, 1247, 1848
BssECI CCNNGG 7 cut(s) 12, 807, 1519, 1813, 1968, 2395, 2544
BssHII GCGCGC 1 cut(s) 307
BssNI GRCGYC 4 cut(s) 132, 219, 411, 1430
BssSI CACGAG 2 cut(s) 1998, 2496
BssT1I CCWWGG 3 cut(s) 807, 1519, 2395
Bst2BI CACGAG 2 cut(s) 1998, 2496
Bst2UI CCWGG 5 cut(s) 1088, 1814, 1959, 2244, 2545
Bst4CI ACNGT 7 cut(s) 994, 1597, 2086, 2093, 2185, 2193, 2423
Bst6I CTCTTC 3 cut(s) 148, 947, 1412
BstACI GRCGYC 4 cut(s) 132, 219, 411, 1430
BstAFI CTTAAG 1 cut(s) 776
BstAUI TGTACA 1 cut(s) 1164
BstBI TTCGAA 1 cut(s) 911
BstDEI CTNAG 7 cut(s) 284, 1495, 1563, 1719, 2071, 2457, 2481
BstDSI CCRYGG 2 cut(s) 807, 2395
BstEII GGTNACC 1 cut(s) 1301
BstF5I GGATG 9 cut(s) 434, 614, 768, 1096, 1231, 1665, 2314, 2359, 2414
BstFNI CGCG 4 cut(s) 273, 307, 309, 2158
BstH2I RGCGCY 3 cut(s) 178, 432, 1570
BstHHI GCGC 7 cut(s) 177, 309, 311, 431, 1189, 1569, 2160
BstMCI CGRYCG 5 cut(s) 58, 253, 489, 631, 2195
BstMWI GCNNNNNNNGC 8 cut(s) 279, 288, 313, 437, 573, 607, 636, 2385
BstNI CCWGG 5 cut(s) 1088, 1814, 1959, 2244, 2545
BstPI GGTNACC 1 cut(s) 1301
BstSFI CTRYAG 3 cut(s) 1901, 1989, 2304
BstUI CGCG 4 cut(s) 273, 307, 309, 2158
BstV1I GCAGC 3 cut(s) 418, 1777, 1832
BstV2I GAAGAC 2 cut(s) 30, 36
BstX2I RGATCY 2 cut(s) 225, 1361
BstXI CCANNNNNNTGG 1 cut(s) 2603
BstYI RGATCY 2 cut(s) 225, 1361
BstZI CGGCCG 2 cut(s) 486, 628
Bsu36I CCTNAGG 1 cut(s) 2457
BtgI CCRYGG 2 cut(s) 807, 2395
BtsCI GGATG 9 cut(s) 434, 614, 768, 1096, 1231, 1665, 2314, 2359, 2414
BtsI GCAGTG 3 cut(s) 418, 1027, 1785
BtsIMutI CAGTG 8 cut(s) 418, 990, 1027, 1074, 1249, 1740, 1785, 2232
BveI ACCTGC 1 cut(s) 1267
CaiI CAGNNNCTG 2 cut(s) 856, 1570
CfoI GCGC 7 cut(s) 177, 309, 311, 431, 1189, 1569, 2160
Cfr10I RCCGGY 4 cut(s) 630, 893, 1247, 1848
CseI GACGC 3 cut(s) 140, 400, 535
Csp6I GTAC 4 cut(s) 214, 702, 1165, 1976
CspCI CAANNNNNGTGG 2 cut(s) 877, 912
CviAII CATG 9 cut(s) 808, 1103, 1374, 1526, 1775, 2126, 2144, 2259, 2396
CviQI GTAC 4 cut(s) 214, 702, 1165, 1976
DdeI CTNAG 7 cut(s) 284, 1495, 1563, 1719, 2071, 2457, 2481
DrdI GACNNNNNNGTC 1 cut(s) 137
DriI GACNNNNNGTC 1 cut(s) 1039
DseDI GACNNNNNNGTC 1 cut(s) 137
EaeI YGGCCR 5 cut(s) 486, 628, 1239, 1810, 2229
EagI CGGCCG 2 cut(s) 486, 628
Eam1104I CTCTTC 3 cut(s) 148, 947, 1412
Eam1105I GACNNNNNGTC 1 cut(s) 1039
EarI CTCTTC 3 cut(s) 148, 947, 1412
EclXI CGGCCG 2 cut(s) 486, 628
Eco130I CCWWGG 3 cut(s) 807, 1519, 2395
Eco24I GRGCYC 1 cut(s) 237
Eco31I GGTCTC 3 cut(s) 485, 491, 497
Eco47I GGWCC 4 cut(s) 396, 581, 789, 1554
Eco47III AGCGCT 1 cut(s) 176
Eco52I CGGCCG 2 cut(s) 486, 628
Eco57I CTGAAG 1 cut(s) 1350
Eco81I CCTNAGG 1 cut(s) 2457
Eco91I GGTNACC 1 cut(s) 1301
EcoO109I RGGNCCY 1 cut(s) 204
EcoO65I GGTNACC 1 cut(s) 1301
EcoRII CCWGG 5 cut(s) 1086, 1812, 1957, 2242, 2543
EcoT14I CCWWGG 3 cut(s) 807, 1519, 2395
EcoT22I ATGCAT 1 cut(s) 1776
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 3 cut(s) 807, 1519, 2395
Esp3I CGTCTC 7 cut(s) 73, 145, 250, 269, 1280, 1387, 2239
FaeI CATG 9 cut(s) 811, 1106, 1377, 1529, 1778, 2129, 2147, 2262, 2399
FalI AAGNNNNNCTT 6 cut(s) 1021, 1053, 1205, 1237, 1609, 1641
FaqI GGGAC 4 cut(s) 34, 802, 1046, 2277
FatI CATG 9 cut(s) 807, 1102, 1373, 1525, 1774, 2125, 2143, 2258, 2395
FauI CCCGC 2 cut(s) 176, 244
FokI GGATG 9 cut(s) 421, 601, 775, 1103, 1238, 1652, 2301, 2366, 2401
FriOI GRGCYC 1 cut(s) 237
FspBI CTAG 7 cut(s) 441, 1823, 1979, 2027, 2163, 2409, 2474
GlaI GCGC 7 cut(s) 176, 308, 310, 430, 1188, 1568, 2159
HaeII RGCGCY 3 cut(s) 178, 432, 1570
HgaI GACGC 3 cut(s) 140, 400, 535
HhaI GCGC 7 cut(s) 177, 309, 311, 431, 1189, 1569, 2160
Hin1I GRCGYC 4 cut(s) 132, 219, 411, 1430
Hin1II CATG 9 cut(s) 811, 1106, 1377, 1529, 1778, 2129, 2147, 2262, 2399
Hin6I GCGC 7 cut(s) 175, 307, 309, 429, 1187, 1567, 2158
HinP1I GCGC 7 cut(s) 175, 307, 309, 429, 1187, 1567, 2158
HincII GTYRAC 1 cut(s) 804
HindII GTYRAC 1 cut(s) 804
HindIII AAGCTT 1 cut(s) 773
HphI GGTGA 8 cut(s) 751, 1262, 1291, 1313, 1592, 2264, 2566, 2591
Hpy166II GTNNAC 6 cut(s) 702, 804, 1600, 2032, 2089, 2360
Hpy8I GTNNAC 6 cut(s) 702, 804, 1600, 2032, 2089, 2360
HpyAV CCTTC 5 cut(s) 594, 721, 1008, 1042, 1291
HpyCH4III ACNGT 7 cut(s) 994, 1597, 2086, 2093, 2185, 2193, 2423
HpyCH4IV ACGT 4 cut(s) 219, 262, 1430, 2450
HpyF10VI GCNNNNNNNGC 8 cut(s) 279, 288, 313, 437, 573, 607, 636, 2385
HpyF3I CTNAG 7 cut(s) 284, 1495, 1563, 1719, 2071, 2457, 2481
HpySE526I ACGT 4 cut(s) 219, 262, 1430, 2450
Hsp92I GRCGYC 4 cut(s) 132, 219, 411, 1430
Hsp92II CATG 9 cut(s) 811, 1106, 1377, 1529, 1778, 2129, 2147, 2262, 2399
HspAI GCGC 7 cut(s) 175, 307, 309, 429, 1187, 1567, 2158
Kpn2I TCCGGA 1 cut(s) 382
KroI GCCGGC 1 cut(s) 630
KroNI GCCGGC 1 cut(s) 632
LmnI GCTCC 9 cut(s) 98, 481, 487, 670, 932, 1017, 1150, 1734, 1842
Lsp1109I GCAGC 3 cut(s) 418, 1777, 1832
LweI GCATC 8 cut(s) 443, 849, 1302, 1674, 1705, 1746, 2378, 2423
MaeI CTAG 7 cut(s) 441, 1823, 1979, 2027, 2163, 2409, 2474
MaeII ACGT 4 cut(s) 219, 262, 1430, 2450
MbiI CCGCTC 2 cut(s) 333, 567
MflI RGATCY 2 cut(s) 225, 1361
MhlI GDGCHC 3 cut(s) 237, 992, 1822
MlsI TGGCCA 2 cut(s) 1241, 1812
MluCI AATT 8 cut(s) 157, 511, 1333, 1935, 2403, 2439, 2583, 2599
MluNI TGGCCA 2 cut(s) 1241, 1812
MlyI GAGTC 3 cut(s) 56, 892, 2010
MmeI TCCRAC 7 cut(s) 138, 273, 324, 1023, 1421, 1807, 2442
Mox20I TGGCCA 2 cut(s) 1241, 1812
Mph1103I ATGCAT 1 cut(s) 1776
MroI TCCGGA 1 cut(s) 382
MroNI GCCGGC 1 cut(s) 630
MroXI GAANNNNTTC 1 cut(s) 2466
MscI TGGCCA 2 cut(s) 1241, 1812
MseI TTAA 4 cut(s) 777, 1212, 1674, 2623
MslI CAYNNNNRTG 1 cut(s) 1530
Msp20I TGGCCA 2 cut(s) 1241, 1812
MspA1I CMGCKG 2 cut(s) 288, 2567
MspCI CTTAAG 1 cut(s) 776
Mva1269I GAATGC 2 cut(s) 60, 919
MvaI CCWGG 5 cut(s) 1088, 1814, 1959, 2244, 2545
MvnI CGCG 4 cut(s) 273, 307, 309, 2158
MwoI GCNNNNNNNGC 8 cut(s) 279, 288, 313, 437, 573, 607, 636, 2385
NaeI GCCGGC 1 cut(s) 632
NciI CCSGG 5 cut(s) 13, 296, 323, 619, 793
NcoI CCATGG 2 cut(s) 807, 2395
NgoMIV GCCGGC 1 cut(s) 630
NlaIII CATG 9 cut(s) 811, 1106, 1377, 1529, 1778, 2129, 2147, 2262, 2399
NlaIV GGNNCC 6 cut(s) 205, 477, 483, 790, 1055, 1831
NmeAIII GCCGAG 2 cut(s) 314, 514
NmuCI GTSAC 5 cut(s) 884, 994, 1301, 1783, 2213
NsiI ATGCAT 1 cut(s) 1776
NspV TTCGAA 1 cut(s) 911
PaqCI CACCTGC 1 cut(s) 1267
PauI GCGCGC 1 cut(s) 307
PcsI WCGNNNNNNNCGW 6 cut(s) 51, 123, 126, 135, 599, 2494
PctI GAATGC 2 cut(s) 60, 919
PdiI GCCGGC 1 cut(s) 632
PdmI GAANNNNTTC 1 cut(s) 2466
PflFI GACNNNGTC 1 cut(s) 410
PfoI TCCNGGA 1 cut(s) 617
Ple19I CGATCG 1 cut(s) 58
PleI GAGTC 3 cut(s) 55, 891, 2009
PpsI GAGTC 3 cut(s) 55, 891, 2009
Psp6I CCWGG 5 cut(s) 1086, 1812, 1957, 2242, 2543
PspEI GGTNACC 1 cut(s) 1301
PspGI CCWGG 5 cut(s) 1086, 1812, 1957, 2242, 2543
PspN4I GGNNCC 6 cut(s) 205, 477, 483, 790, 1055, 1831
PstI CTGCAG 2 cut(s) 1905, 1993
PstNI CAGNNNCTG 2 cut(s) 856, 1570
PsuI RGATCY 2 cut(s) 225, 1361
PsyI GACNNNGTC 1 cut(s) 410
PteI GCGCGC 1 cut(s) 307
PvuI CGATCG 1 cut(s) 58
RsaI GTAC 4 cut(s) 215, 703, 1166, 1977
RsaNI GTAC 4 cut(s) 214, 702, 1165, 1976
RseI CAYNNNNRTG 1 cut(s) 1530
SaqAI TTAA 4 cut(s) 777, 1212, 1674, 2623
ScaI AGTACT 1 cut(s) 1977
SchI GAGTC 3 cut(s) 56, 892, 2010
SduI GDGCHC 3 cut(s) 237, 992, 1822
SfaNI GCATC 8 cut(s) 443, 849, 1302, 1674, 1705, 1746, 2378, 2423
SfcI CTRYAG 3 cut(s) 1901, 1989, 2304
SfuI TTCGAA 1 cut(s) 911
SinI GGWCC 4 cut(s) 396, 581, 789, 1554
SmiMI CAYNNNNRTG 1 cut(s) 1530
SmlI CTYRAG 1 cut(s) 776
SmoI CTYRAG 1 cut(s) 776
SpeI ACTAGT 2 cut(s) 1978, 2408
Sse9I AATT 8 cut(s) 157, 511, 1333, 1935, 2403, 2439, 2583, 2599
SspMI CTAG 7 cut(s) 441, 1823, 1979, 2027, 2163, 2409, 2474
StyI CCWWGG 3 cut(s) 807, 1519, 2395
TaaI ACNGT 7 cut(s) 994, 1597, 2086, 2093, 2185, 2193, 2423
TaiI ACGT 4 cut(s) 222, 265, 1433, 2453
TaqI TCGA 4 cut(s) 54, 414, 911, 2013
TaqII GACCGA 2 cut(s) 509, 515
TasI AATT 8 cut(s) 157, 511, 1333, 1935, 2403, 2439, 2583, 2599
TatI WGTACW 3 cut(s) 701, 1164, 1975
Tru1I TTAA 4 cut(s) 777, 1212, 1674, 2623
Tru9I TTAA 4 cut(s) 777, 1212, 1674, 2623
TscAI CASTG 8 cut(s) 425, 997, 1027, 1081, 1249, 1747, 1792, 2232
TseFI GTSAC 5 cut(s) 884, 994, 1301, 1783, 2213
TseI GCWGC 3 cut(s) 431, 1790, 1845
Tsp45I GTSAC 5 cut(s) 884, 994, 1301, 1783, 2213
TspDTI ATGAA 8 cut(s) 1041, 1091, 1256, 1503, 1687, 2160, 2198, 2247
TspGWI ACGGA 4 cut(s) 232, 579, 1304, 1369
TspRI CASTG 8 cut(s) 425, 997, 1027, 1081, 1249, 1747, 1792, 2232
Tth111I GACNNNGTC 1 cut(s) 410
Vha464I CTTAAG 1 cut(s) 776
VpaK11BI GGWCC 4 cut(s) 396, 581, 789, 1554
XapI RAATTY 3 cut(s) 511, 2439, 2599
XmnI GAANNNNTTC 1 cut(s) 2466
XspI CTAG 7 cut(s) 441, 1823, 1979, 2027, 2163, 2409, 2474
ZraI GACGTC 2 cut(s) 220, 1431
ZrmI AGTACT 1 cut(s) 1977
Zsp2I ATGCAT 1 cut(s) 1776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.