FvH4_3g29511

MULE transposase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013646
Physical Location & Seq
Forward (+)
22562922 .. 22565194
2273 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29511.t1

Sequence Viewer

Length: 1311 bp
ATGGAGATAGTTGATAACAAAGATGTTGCTAGTTGGGTGTATGACAGCCGTTCGGTGCAACCTAACAAGATTCCATTATACATTACTTTGGAACGCAGCTTGTCAAGATCTGTAAGTGAAGGCAGTCAAGACATGAACAATAGATCTTACCCGTCTTTTCAGTATGATGATGTTGGCTTAACCATTTTCTCTACTGAACTTGATGATCTGAGCCCGATTGCAATGAGTCCCCCACATGAGCAAATAATAGTAAACTCTGATTTGGATAATGCTGATAATGTCTCGTATGGAAGTACAGAGGAAGATGAGTTTTCACTATCCAATGGGGATAATGAGAATCCAATGCATGGTGTAGAAATCAATGTTAGTATGCAACCTACGGATGACAACTTTGCTTCATCATCTTCTTATCGTAGCAAGAAAAGGCGAATAGACGATTTTGAAATTGTTACATTTGTGGACACCAATGAAGATATTTTGGTAGGTCAGTTATACCCGAGCAAGAAAGCCTTGAAACAACACTTGGCGGCAAAGAAGTACCAGGTGTCCGAGTTCAATACATTGATGGATGACATTCGAAAAATAAAAAATGGAGAAGTTTGTAAATATCTTGAAGACATTGGGTGTCATAAGTGGGCAAGAGCTCATTTCATGGGGTACAGATACAACATGATGACATCCAACATAGCCGAATCTATGAACGCGAAGCTTAAAGAGGCTAGAAAATTACCAATAACTGCTCTAGTGGATCACCTAAGAGAAGTGCTTCAACAATGGTTTGTTGAACGACGTGATGCAGCAAGCTCCCGTAATACACACTTAACCAAGTGGGCTGAAAACAAAGTGCGAAAAAACAACAACAGTGGTTTGCATATGCGGGTGTCTCCAATTGATCTTTATGCATTTCAAATACATGATCAAAGTCAAACATTTACAGTGGACCTGAATAACATGACCTGCACATGTCGAGAGTTTGATCTTGATCGACTTCCTTGTGCCCATGCGACAGTTGCTTGTAGATTTAAAGGAGTATCAGTGTATAGCATGTGTTCTCAATTTTACACGGCCAATGCACTCATGTTAGCATATGCAGAGCCTATTTGGTCAGTTGGAAATAAAAGTGAGTGGGATGTGTCTCAAGATGTGCAGAACAAAATTGTCTTACCACCAATAAGACAAGTAGTACCCGGAAGACGTAAGACGGTACGAATTCCATATGTGGGAGAGGATGCAATTAGAAAAAAGTGTACAAGATGCGGTGGAGCAGGTCATAACCGTGCAACTTGCAAAAATCCAATTCCACTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

437

Amino Acids

49.65

Weight (kDa)

6.43

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 965, 1256
AccB7I CCANNNNNTGG 1 cut(s) 347
AccII CGCG 1 cut(s) 704
AciI CCGC 3 cut(s) 527, 877, 1257
AclWI GGATC 1 cut(s) 756
AcoI YGGCCR 1 cut(s) 1065
AcsI RAATTY 1 cut(s) 1209
AfaI GTAC 6 cut(s) 295, 539, 659, 1185, 1206, 1249
AfiI CCNNNNNNNGG 2 cut(s) 347, 1220
AflIII ACRYGT 1 cut(s) 962
AgsI TTSAA 7 cut(s) 443, 514, 556, 614, 770, 785, 908
AjiI CACGTC 1 cut(s) 791
AjnI CCWGG 1 cut(s) 540
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AluBI AGCT 4 cut(s) 99, 644, 709, 804
AluI AGCT 4 cut(s) 99, 644, 709, 804
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 3 cut(s) 286, 888, 1140
AlwI GGATC 1 cut(s) 756
Ama87I CYCGRG 1 cut(s) 496
AoxI GGCC 1 cut(s) 1065
ApeKI GCWGC 2 cut(s) 96, 797
ApoI RAATTY 1 cut(s) 1209
Asp700I GAANNNNTTC 1 cut(s) 765
AspS9I GGNCC 1 cut(s) 940
AsuC2I CCSGG 1 cut(s) 1188
AsuHPI GGTGA 1 cut(s) 743
AsuII TTCGAA 1 cut(s) 577
AvaI CYCGRG 1 cut(s) 496
AvaII GGWCC 1 cut(s) 940
BaeGI GKGCMC 1 cut(s) 1000
BanII GRGCYC 2 cut(s) 215, 646
BbsI GAAGAC 2 cut(s) 621, 1198
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 2 cut(s) 108, 809
BccI CCATC 1 cut(s) 559
BceAI ACGGC 2 cut(s) 33, 1080
BciT130I CCWGG 1 cut(s) 542
BclI TGATCA 1 cut(s) 916
BcnI CCSGG 1 cut(s) 1188
BcoDI GTCTC 3 cut(s) 286, 888, 1140
BfaI CTAG 3 cut(s) 30, 720, 743
BfuAI ACCTGC 2 cut(s) 965, 1256
BglII AGATCT 2 cut(s) 107, 143
BisI GCNGC 3 cut(s) 97, 528, 798
BlsI GCNGC 3 cut(s) 98, 529, 799
Bme1390I CCNGG 2 cut(s) 542, 1188
Bme18I GGWCC 1 cut(s) 940
BmeT110I CYCGRG 1 cut(s) 496
BmgBI CACGTC 1 cut(s) 791
BmgT120I GGNCC 1 cut(s) 940
BmrFI CCNGG 2 cut(s) 542, 1188
BmsI GCATC 3 cut(s) 784, 1219, 1244
BpiI GAAGAC 2 cut(s) 621, 1198
Bpu14I TTCGAA 1 cut(s) 577
BpuEI CTTGAG 1 cut(s) 1122
BpuMI CCSGG 1 cut(s) 1188
BsaBI GATNNNNATC 1 cut(s) 981
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 1220
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 981
BseBI CCWGG 1 cut(s) 542
BseGI GGATG 5 cut(s) 388, 574, 677, 1135, 1234
BseJI GATNNNNATC 1 cut(s) 981
BseLI CCNNNNNNNGG 2 cut(s) 347, 1220
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 200
BseSI GKGCMC 1 cut(s) 1000
BseXI GCAGC 2 cut(s) 108, 809
BsgI GTGCAG 2 cut(s) 943, 1166
Bsh1236I CGCG 1 cut(s) 704
BshFI GGCC 1 cut(s) 1067
BsiHKAI GWGCWC 1 cut(s) 646
BsiHKCI CYCGRG 1 cut(s) 496
BsiSI CCGG 1 cut(s) 1188
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 2 cut(s) 347, 1220
BsmAI GTCTC 3 cut(s) 286, 888, 1140
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 1 cut(s) 1067
BsoBI CYCGRG 1 cut(s) 496
Bsp119I TTCGAA 1 cut(s) 577
Bsp1286I GDGCHC 3 cut(s) 215, 646, 1000
Bsp1407I TGTACA 1 cut(s) 1247
Bsp143I GATC 8 cut(s) 107, 143, 205, 748, 892, 916, 976, 982
BspACI CCGC 3 cut(s) 527, 877, 1257
BspANI GGCC 1 cut(s) 1067
BspCNI CTCAG 1 cut(s) 201
BspFNI CGCG 1 cut(s) 704
BspMI ACCTGC 2 cut(s) 965, 1256
BspPI GGATC 1 cut(s) 756
BspT104I TTCGAA 1 cut(s) 577
BsrDI GCAATG 1 cut(s) 228
BsrGI TGTACA 1 cut(s) 1247
BssMI GATC 8 cut(s) 107, 143, 205, 748, 892, 916, 976, 982
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 5 cut(s) 863, 937, 1009, 1204, 1277
BstAUI TGTACA 1 cut(s) 1247
BstBI TTCGAA 1 cut(s) 577
BstC8I GCNNGC 1 cut(s) 802
BstDEI CTNAG 2 cut(s) 209, 755
BstF5I GGATG 5 cut(s) 388, 574, 677, 1135, 1234
BstFNI CGCG 1 cut(s) 704
BstKTI GATC 8 cut(s) 110, 146, 208, 751, 895, 919, 979, 985
BstMAI GTCTC 3 cut(s) 286, 888, 1140
BstMBI GATC 8 cut(s) 107, 143, 205, 748, 892, 916, 976, 982
BstMWI GCNNNNNNNGC 1 cut(s) 1010
BstNI CCWGG 1 cut(s) 542
BstNSI RCATGY 2 cut(s) 966, 1048
BstSCI CCNGG 2 cut(s) 540, 1186
BstSLI GKGCMC 1 cut(s) 1000
BstUI CGCG 1 cut(s) 704
BstV1I GCAGC 2 cut(s) 108, 809
BstV2I GAAGAC 2 cut(s) 621, 1198
BstX2I RGATCY 2 cut(s) 107, 143
BstYI RGATCY 2 cut(s) 107, 143
BsuRI GGCC 1 cut(s) 1067
BtrI CACGTC 1 cut(s) 791
BtsCI GGATG 5 cut(s) 388, 574, 677, 1135, 1234
BtsIMutI CAGTG 3 cut(s) 868, 942, 1041
BveI ACCTGC 2 cut(s) 965, 1256
Cac8I GCNNGC 1 cut(s) 802
Cfr13I GGNCC 1 cut(s) 940
CsiI ACCWGGT 1 cut(s) 540
Csp6I GTAC 6 cut(s) 294, 538, 658, 1184, 1205, 1248
CspCI CAANNNNNGTGG 2 cut(s) 844, 879
CviQI GTAC 6 cut(s) 294, 538, 658, 1184, 1205, 1248
DdeI CTNAG 2 cut(s) 209, 755
DpnI GATC 8 cut(s) 109, 145, 207, 750, 894, 918, 978, 984
DpnII GATC 8 cut(s) 107, 143, 205, 748, 892, 916, 976, 982
DraI TTTAAA 1 cut(s) 1024
EaeI YGGCCR 1 cut(s) 1065
Ecl136II GAGCTC 1 cut(s) 644
Eco24I GRGCYC 2 cut(s) 215, 646
Eco47I GGWCC 1 cut(s) 940
Eco53kI GAGCTC 1 cut(s) 644
Eco88I CYCGRG 1 cut(s) 496
EcoICRI GAGCTC 1 cut(s) 644
EcoRI GAATTC 1 cut(s) 1209
EcoRII CCWGG 1 cut(s) 540
EcoT22I ATGCAT 2 cut(s) 348, 904
EcoT38I GRGCYC 2 cut(s) 215, 646
FalI AAGNNNNNCTT 2 cut(s) 494, 526
FaqI GGGAC 1 cut(s) 213
FauI CCCGC 1 cut(s) 870
FauNDI CATATG 3 cut(s) 873, 1087, 1216
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 3 cut(s) 97, 528, 798
FokI GGATG 5 cut(s) 395, 581, 664, 1142, 1241
FriOI GRGCYC 2 cut(s) 215, 646
Fsp4HI GCNGC 3 cut(s) 97, 528, 798
FspBI CTAG 3 cut(s) 30, 720, 743
GluI GCNGC 3 cut(s) 97, 528, 798
HaeIII GGCC 1 cut(s) 1067
HapII CCGG 1 cut(s) 1188
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 4 cut(s) 70, 226, 337, 692
HpaII CCGG 1 cut(s) 1188
HphI GGTGA 1 cut(s) 743
Hpy166II GTNNAC 4 cut(s) 253, 460, 940, 1248
Hpy188I TCNGA 3 cut(s) 210, 259, 550
Hpy188III TCNNGA 6 cut(s) 105, 128, 611, 968, 980, 1139
Hpy8I GTNNAC 4 cut(s) 253, 460, 940, 1248
Hpy99I CGWCG 1 cut(s) 792
HpyAV CCTTC 1 cut(s) 113
HpyCH4III ACNGT 5 cut(s) 863, 937, 1009, 1204, 1277
HpyCH4IV ACGT 2 cut(s) 790, 1195
HpyF10VI GCNNNNNNNGC 1 cut(s) 1010
HpyF3I CTNAG 2 cut(s) 209, 755
HpySE526I ACGT 2 cut(s) 790, 1195
Ksp22I TGATCA 1 cut(s) 916
Kzo9I GATC 8 cut(s) 107, 143, 205, 748, 892, 916, 976, 982
LmnI GCTCC 2 cut(s) 809, 1262
LpnPI CCDG 6 cut(s) 527, 554, 956, 970, 1201, 1251
Lsp1109I GCAGC 2 cut(s) 108, 809
LweI GCATC 3 cut(s) 784, 1219, 1244
MabI ACCWGGT 1 cut(s) 540
MaeI CTAG 3 cut(s) 30, 720, 743
MaeII ACGT 2 cut(s) 790, 1195
MaeIII GTNAC 1 cut(s) 448
MalI GATC 8 cut(s) 109, 145, 207, 750, 894, 918, 978, 984
MboI GATC 8 cut(s) 107, 143, 205, 748, 892, 916, 976, 982
MboII GAAGA 5 cut(s) 314, 396, 482, 626, 1203
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 2 cut(s) 107, 143
MhlI GDGCHC 3 cut(s) 215, 646, 1000
MluCI AATT 9 cut(s) 444, 725, 888, 1055, 1155, 1209, 1233, 1296, 1306
MlyI GAGTC 1 cut(s) 235
MmeI TCCRAC 2 cut(s) 705, 1090
MnlI CCTC 3 cut(s) 292, 709, 1219
Mph1103I ATGCAT 2 cut(s) 348, 904
MroXI GAANNNNTTC 1 cut(s) 765
MseI TTAA 5 cut(s) 179, 711, 821, 1023, 1305
MslI CAYNNNNRTG 1 cut(s) 1275
MspI CCGG 1 cut(s) 1188
MspR9I CCNGG 2 cut(s) 542, 1188
MunI CAATTG 1 cut(s) 888
MvaI CCWGG 1 cut(s) 542
MvnI CGCG 1 cut(s) 704
MwoI GCNNNNNNNGC 1 cut(s) 1010
NciI CCSGG 1 cut(s) 1188
NdeI CATATG 3 cut(s) 873, 1087, 1216
NdeII GATC 8 cut(s) 107, 143, 205, 748, 892, 916, 976, 982
NsiI ATGCAT 2 cut(s) 348, 904
NspI RCATGY 2 cut(s) 966, 1048
NspV TTCGAA 1 cut(s) 577
PciI ACATGT 1 cut(s) 962
PdmI GAANNNNTTC 1 cut(s) 765
PfeI GAWTC 3 cut(s) 70, 337, 692
PflMI CCANNNNNTGG 1 cut(s) 347
PkrI GCNGC 3 cut(s) 98, 529, 799
PleI GAGTC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 234
PscI ACATGT 1 cut(s) 962
Psp124BI GAGCTC 1 cut(s) 646
Psp6I CCWGG 1 cut(s) 540
PspGI CCWGG 1 cut(s) 540
PspPI GGNCC 1 cut(s) 940
PsuI RGATCY 2 cut(s) 107, 143
RsaI GTAC 6 cut(s) 295, 539, 659, 1185, 1206, 1249
RsaNI GTAC 6 cut(s) 294, 538, 658, 1184, 1205, 1248
RseI CAYNNNNRTG 1 cut(s) 1275
SacI GAGCTC 1 cut(s) 646
SaqAI TTAA 5 cut(s) 179, 711, 821, 1023, 1305
SatI GCNGC 3 cut(s) 97, 528, 798
Sau3AI GATC 8 cut(s) 107, 143, 205, 748, 892, 916, 976, 982
Sau96I GGNCC 1 cut(s) 940
SchI GAGTC 1 cut(s) 235
ScrFI CCNGG 2 cut(s) 542, 1188
SduI GDGCHC 3 cut(s) 215, 646, 1000
SexAI ACCWGGT 1 cut(s) 540
SfaNI GCATC 3 cut(s) 784, 1219, 1244
SfuI TTCGAA 1 cut(s) 577
SinI GGWCC 1 cut(s) 940
SmiMI CAYNNNNRTG 1 cut(s) 1275
SmlI CTYRAG 1 cut(s) 1137
SmoI CTYRAG 1 cut(s) 1137
Sse9I AATT 9 cut(s) 444, 725, 888, 1055, 1155, 1209, 1233, 1296, 1306
SsiI CCGC 3 cut(s) 527, 877, 1257
SspMI CTAG 3 cut(s) 30, 720, 743
SstI GAGCTC 1 cut(s) 646
StyD4I CCNGG 2 cut(s) 540, 1186
TaaI ACNGT 5 cut(s) 863, 937, 1009, 1204, 1277
TaiI ACGT 2 cut(s) 793, 1198
TaqI TCGA 3 cut(s) 577, 967, 985
TasI AATT 9 cut(s) 444, 725, 888, 1055, 1155, 1209, 1233, 1296, 1306
TatI WGTACW 2 cut(s) 293, 1247
TauI GCSGC 1 cut(s) 530
TfiI GAWTC 3 cut(s) 70, 337, 692
Tru1I TTAA 5 cut(s) 179, 711, 821, 1023, 1305
Tru9I TTAA 5 cut(s) 179, 711, 821, 1023, 1305
TscAI CASTG 3 cut(s) 868, 942, 1041
TseI GCWGC 2 cut(s) 96, 797
TspDTI ATGAA 5 cut(s) 149, 387, 483, 640, 713
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 3 cut(s) 868, 942, 1041
Van91I CCANNNNNTGG 1 cut(s) 347
VpaK11BI GGWCC 1 cut(s) 940
XapI RAATTY 1 cut(s) 1209
XceI RCATGY 2 cut(s) 966, 1048
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 3 cut(s) 30, 720, 743
Zsp2I ATGCAT 2 cut(s) 348, 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.