Rorug06G0053400

MULE transposase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006435
Physical Location & Seq
Forward (+)
7412166 .. 7414629
2464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0053400.1

Sequence Viewer

Length: 585 bp
ATGTCCGAGAATCCATTGCAGGCACTCTTCCAAACTTTCGCAAAACTCTCAAACTCAGTTCAGACCCACCTCTCTAATCTCATACCCCAACCTCCTCACCCCACACCCAAAACCCCCTCAATCTCCATCTCTTCCTCCTCCAAAGTTTCCCCATCACAGAATGACCCCACCCATCTCCAACCCCAAGATCTCATCAACAACAAGGGAAAATCGGCTGGTCCTGTGACGAAAGAAGGGCTCGGAAGAGCCACTTGGACTTTTCTTCACACTCTTGCTGCTCAGTATCCGGATAATCCAACGAGGCAACAGAAGAAGGATGTAAAAGAATTGATGTCAATACTATCACGAATGTACCCCTGCAAGGAATGTGCAGATCATTTTCAAGAAATTCTGAGATCAAATCCTGTACAGGCTGGATCTCATGCTGAATTCTCCCAATGGCTATGTCATGTGCATAATACTGTCAACAGAAGCCTTAACAAAGTGGTTTTCCCCTGTGAACGAGTTGATGCAAGGTGGGGCAAGTTAGAGTGCGAACAGCGTGCTTGCGATGTACTAGGAAGTACCACAAGTTTTTGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

194

Amino Acids

21.73

Weight (kDa)

8.94

Isoelectric Point (pI)

60.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Evr1_Alr PF04777 82 - 173 6e-34 Erv1 / Alr family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 286
AclWI GGATC 1 cut(s) 424
AcsI RAATTY 2 cut(s) 387, 428
AfaI GTAC 4 cut(s) 353, 408, 555, 565
AfiI CCNNNNNNNGG 1 cut(s) 361
AgsI TTSAA 1 cut(s) 383
AjuI GAANNNNNNNTTGG 2 cut(s) 235, 267
AlwI GGATC 1 cut(s) 424
Aor13HI TCCGGA 1 cut(s) 286
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 2 cut(s) 387, 428
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 1 cut(s) 218
BaeI ACNNNNGTAYC 2 cut(s) 335, 368
BanII GRGCYC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 262
BccI CCATC 3 cut(s) 134, 160, 180
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciVI GTATCC 1 cut(s) 294
BfaI CTAG 1 cut(s) 557
BfuI GTATCC 1 cut(s) 294
BglII AGATCT 1 cut(s) 187
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 218
BmsI GCATC 1 cut(s) 499
BsaWI WCCGGW 1 cut(s) 286
Bsc4I CCNNNNNNNGG 1 cut(s) 361
Bse3DI GCAATG 1 cut(s) 14
BseAI TCCGGA 1 cut(s) 286
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 361
BseMI GCAATG 1 cut(s) 14
BseMII CTCAG 3 cut(s) 69, 293, 383
BseRI GAGGAG 2 cut(s) 84, 127
BseXI GCAGC 1 cut(s) 262
BsgI GTGCAG 1 cut(s) 390
BsiSI CCGG 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 240
Bsp13I TCCGGA 1 cut(s) 286
Bsp1407I TGTACA 1 cut(s) 406
Bsp143I GATC 4 cut(s) 187, 373, 395, 416
BspCNI CTCAG 3 cut(s) 68, 292, 384
BspEI TCCGGA 1 cut(s) 286
BspPI GGATC 1 cut(s) 424
BspQI GCTCTTC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 406
BssMI GATC 4 cut(s) 187, 373, 395, 416
Bst4CI ACNGT 1 cut(s) 463
Bst6I CTCTTC 3 cut(s) 32, 136, 238
BstAUI TGTACA 1 cut(s) 406
BstC8I GCNNGC 3 cut(s) 21, 543, 547
BstDEI CTNAG 3 cut(s) 55, 279, 392
BstF5I GGATG 1 cut(s) 322
BstKTI GATC 4 cut(s) 190, 376, 398, 419
BstMBI GATC 4 cut(s) 187, 373, 395, 416
BstV1I GCAGC 1 cut(s) 262
BstX2I RGATCY 2 cut(s) 187, 416
BstYI RGATCY 2 cut(s) 187, 416
BsuI GTATCC 1 cut(s) 294
BtgZI GCGATG 1 cut(s) 564
BtsCI GGATG 1 cut(s) 322
Cac8I GCNNGC 3 cut(s) 21, 543, 547
Cfr13I GGNCC 1 cut(s) 218
Csp6I GTAC 4 cut(s) 352, 407, 554, 564
CviAII CATG 2 cut(s) 422, 449
CviJI RGCY 6 cut(s) 215, 238, 248, 413, 442, 474
CviKI_1 RGCY 6 cut(s) 215, 238, 248, 413, 442, 474
CviQI GTAC 4 cut(s) 352, 407, 554, 564
DdeI CTNAG 3 cut(s) 55, 279, 392
DpnI GATC 4 cut(s) 189, 375, 397, 418
DpnII GATC 4 cut(s) 187, 373, 395, 416
Eam1104I CTCTTC 3 cut(s) 32, 136, 238
EarI CTCTTC 3 cut(s) 32, 136, 238
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 218
EcoRI GAATTC 1 cut(s) 428
EcoT38I GRGCYC 1 cut(s) 240
FaeI CATG 2 cut(s) 425, 452
FaiI YATR 5 cut(s) 83, 423, 445, 450, 456
FalI AAGNNNNNCTT 2 cut(s) 235, 267
FatI CATG 2 cut(s) 421, 448
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 1 cut(s) 329
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 276
FspBI CTAG 1 cut(s) 557
GluI GCNGC 1 cut(s) 276
HapII CCGG 1 cut(s) 287
Hin1II CATG 2 cut(s) 425, 452
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 1 cut(s) 10
HpaII CCGG 1 cut(s) 287
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 2 cut(s) 466, 500
Hpy188I TCNGA 4 cut(s) 7, 63, 242, 393
Hpy188III TCNNGA 3 cut(s) 287, 345, 383
Hpy8I GTNNAC 2 cut(s) 466, 500
HpyAV CCTTC 2 cut(s) 227, 307
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4V TGCA 5 cut(s) 19, 360, 371, 454, 512
HpyF3I CTNAG 3 cut(s) 55, 279, 392
Hsp92II CATG 2 cut(s) 425, 452
Kpn2I TCCGGA 1 cut(s) 286
Kzo9I GATC 4 cut(s) 187, 373, 395, 416
LguI GCTCTTC 1 cut(s) 238
LpnPI CCDG 9 cut(s) 5, 201, 234, 300, 370, 395, 399, 417, 508
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 1 cut(s) 499
MaeI CTAG 1 cut(s) 557
MaeIII GTNAC 1 cut(s) 223
MalI GATC 4 cut(s) 189, 375, 397, 418
MboI GATC 4 cut(s) 187, 373, 395, 416
MboII GAAGA 5 cut(s) 19, 123, 254, 255, 322
MflI RGATCY 2 cut(s) 187, 416
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 3 cut(s) 326, 387, 428
MmeI TCCRAC 2 cut(s) 202, 320
MnlI CCTC 7 cut(s) 80, 102, 105, 127, 145, 148, 294
MroI TCCGGA 1 cut(s) 286
MseI TTAA 1 cut(s) 477
MspI CCGG 1 cut(s) 287
NdeII GATC 4 cut(s) 187, 373, 395, 416
NlaIII CATG 2 cut(s) 425, 452
NmuCI GTSAC 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 277
PspPI GGNCC 1 cut(s) 218
PsuI RGATCY 2 cut(s) 187, 416
RsaI GTAC 4 cut(s) 353, 408, 555, 565
RsaNI GTAC 4 cut(s) 352, 407, 554, 564
SapI GCTCTTC 1 cut(s) 238
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 4 cut(s) 187, 373, 395, 416
Sau96I GGNCC 1 cut(s) 218
SduI GDGCHC 1 cut(s) 240
SetI ASST 3 cut(s) 72, 94, 518
SfaNI GCATC 1 cut(s) 499
SinI GGWCC 1 cut(s) 218
Sse9I AATT 3 cut(s) 326, 387, 428
SspMI CTAG 1 cut(s) 557
TaaI ACNGT 1 cut(s) 463
TasI AATT 3 cut(s) 326, 387, 428
TatI WGTACW 2 cut(s) 406, 553
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 223
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 2 cut(s) 387, 428
XspI CTAG 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.