FvH4_6g24082

MULE transposase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013646
Physical Location & Seq
Forward (+)
18109917 .. 18111255
1339 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g24082.t1

Sequence Viewer

Length: 957 bp
ATGGAGATAGCGGACAATGAAGATGTAGCTAGTTGGGTGTGTGAGAGCCTTTCCATTCAATCTAACAAGATTCCATTATATATTACTTTGGAGCAGAGCTTGTCAAGATCTATAAGTGAAAGTAATCAAGACTTGACCAATAGACCTAACCGATCACAATATGTTGATGTTGTCTCAATGATTTTCTCTACTGAAGTTGAAGATCAACACCCAATTGCTTTGAGTTCCCCACATGAGCCATTAGTAGTAAACTCTGCGTTGGAGAATGGTGATAATGTCTCATACGAAAATAGAGAGGAAGATGAGTTTCCACATTCCAATTGGGATAATGAGGATCAAATGGACAATGTAGAAATTGGAAGTGACCAACCTGAGATTGTGAAGCTCTGCTCCATCATCGTCTTATTACCTAGAGACATCATTGATGATGCTCGTGTTCAACTTTGTGTTAATATGGGTTCTAATATTCAACTTTGTGTTAATATGGGTTATAATAAAGCTTGGAGGGCAAGAGAAGCAGCACTAGAAATTGCAAGAGGATCGCCAGAGGAATCATTTGCTCAACTTCGAGCATATTGTGCTATGTTAGAGCGTCGAAACCAAGATACAATAACACACATAGAAAATGATGATCATAATCACTTTCTATGCTTTTTCATGGCAATTGGAGCCTCAATTAGAGGATTTCGTGATTCTATGAGGCCTGTTATTGCTATAGATGGTACATTTTTGAAAAGAAAGTACTTTGGAACCATGTTAATGGCTGCATGCCAAGATGGAGAGAATCAAATATATCCTTTGGCATTTGGGGTGGTAGACTTAGAAAATGATGCCTCATGGTCATGGTTTTTGACAAAATTAAGAGGGGCCATTGGAGTTGTAGAGAACCTTGTGTTCATATCTGATAGGTACGGAAGCATCGCCAAAGTTGTGGATAACGTTTTTCCTGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

319

Amino Acids

35.96

Weight (kDa)

4.47

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MULE PF10551 236 - 317 1.7e-14 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 492
AccI GTMKAC 1 cut(s) 816
AciI CCGC 1 cut(s) 11
AclI AACGTT 1 cut(s) 939
AclWI GGATC 2 cut(s) 342, 547
AcuI CTGAAG 1 cut(s) 213
AfaI GTAC 3 cut(s) 724, 743, 911
AgsI TTSAA 5 cut(s) 59, 200, 440, 470, 733
AluBI AGCT 4 cut(s) 29, 99, 385, 500
AluI AGCT 4 cut(s) 29, 99, 385, 500
Alw26I GTCTC 3 cut(s) 178, 283, 408
AlwI GGATC 2 cut(s) 342, 547
AoxI GGCC 2 cut(s) 701, 867
ApeKI GCWGC 2 cut(s) 518, 764
AspS9I GGNCC 1 cut(s) 867
AsuHPI GGTGA 1 cut(s) 281
BauI CACGAG 1 cut(s) 432
BbvI GCAGC 2 cut(s) 530, 751
BccI CCATC 3 cut(s) 401, 713, 770
BcgI CGANNNNNNTGC 2 cut(s) 522, 556
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 3 cut(s) 178, 283, 408
BfaI CTAG 3 cut(s) 30, 411, 524
BfmI CTRYAG 1 cut(s) 714
BglII AGATCT 1 cut(s) 107
BisI GCNGC 2 cut(s) 519, 765
BlsI GCNGC 2 cut(s) 520, 766
BmcAI AGTACT 1 cut(s) 743
BmgT120I GGNCC 1 cut(s) 867
BmiI GGNNCC 3 cut(s) 670, 751, 868
BmsI GCATC 3 cut(s) 418, 820, 927
BsaBI GATNNNNATC 1 cut(s) 636
Bse8I GATNNNNATC 1 cut(s) 636
BseJI GATNNNNATC 1 cut(s) 636
BseMII CTCAG 1 cut(s) 363
BseXI GCAGC 2 cut(s) 530, 751
BshFI GGCC 2 cut(s) 703, 869
BsmAI GTCTC 3 cut(s) 178, 283, 408
BsnI GGCC 2 cut(s) 703, 869
Bsp143I GATC 6 cut(s) 107, 152, 202, 334, 539, 631
BspACI CCGC 1 cut(s) 11
BspANI GGCC 2 cut(s) 703, 869
BspCNI CTCAG 1 cut(s) 364
BspLI GGNNCC 3 cut(s) 670, 751, 868
BspPI GGATC 2 cut(s) 342, 547
BssMI GATC 6 cut(s) 107, 152, 202, 334, 539, 631
BssSI CACGAG 1 cut(s) 432
Bst2BI CACGAG 1 cut(s) 432
BstAPI GCANNNNNTGC 1 cut(s) 578
BstC8I GCNNGC 1 cut(s) 769
BstDEI CTNAG 2 cut(s) 372, 820
BstKTI GATC 6 cut(s) 110, 155, 205, 337, 542, 634
BstMAI GTCTC 3 cut(s) 178, 283, 408
BstMBI GATC 6 cut(s) 107, 152, 202, 334, 539, 631
BstMWI GCNNNNNNNGC 4 cut(s) 506, 515, 578, 668
BstNSI RCATGY 1 cut(s) 771
BstSFI CTRYAG 1 cut(s) 714
BstV1I GCAGC 2 cut(s) 530, 751
BstX2I RGATCY 1 cut(s) 107
BstXI CCANNNNNNTGG 2 cut(s) 760, 931
BstYI RGATCY 1 cut(s) 107
BsuRI GGCC 2 cut(s) 703, 869
BtgZI GCGATG 1 cut(s) 904
Cac8I GCNNGC 1 cut(s) 769
Cfr13I GGNCC 1 cut(s) 867
CseI GACGC 1 cut(s) 581
Csp6I GTAC 3 cut(s) 723, 742, 910
CviAII CATG 6 cut(s) 233, 658, 754, 768, 837, 843
CviQI GTAC 3 cut(s) 723, 742, 910
DdeI CTNAG 2 cut(s) 372, 820
DpnI GATC 6 cut(s) 109, 154, 204, 336, 541, 633
DpnII GATC 6 cut(s) 107, 152, 202, 334, 539, 631
Eco147I AGGCCT 1 cut(s) 703
Eco57I CTGAAG 1 cut(s) 213
FaeI CATG 6 cut(s) 236, 661, 757, 771, 840, 846
FatI CATG 6 cut(s) 232, 657, 753, 767, 836, 842
FbaI TGATCA 1 cut(s) 631
FblI GTMKAC 1 cut(s) 816
Fnu4HI GCNGC 2 cut(s) 519, 765
Fsp4HI GCNGC 2 cut(s) 519, 765
FspBI CTAG 3 cut(s) 30, 411, 524
GluI GCNGC 2 cut(s) 519, 765
HaeIII GGCC 2 cut(s) 703, 869
HgaI GACGC 1 cut(s) 581
Hin1II CATG 6 cut(s) 236, 661, 757, 771, 840, 846
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 4 cut(s) 70, 551, 692, 784
HphI GGTGA 1 cut(s) 281
Hpy166II GTNNAC 2 cut(s) 250, 817
Hpy188I TCNGA 1 cut(s) 904
Hpy188III TCNNGA 4 cut(s) 105, 128, 689, 947
Hpy8I GTNNAC 2 cut(s) 250, 817
Hpy99I CGWCG 1 cut(s) 597
HpyCH4IV ACGT 1 cut(s) 939
HpyCH4V TGCA 2 cut(s) 533, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 506, 515, 578, 668
HpyF3I CTNAG 2 cut(s) 372, 820
HpySE526I ACGT 1 cut(s) 939
Hsp92II CATG 6 cut(s) 236, 661, 757, 771, 840, 846
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 6 cut(s) 107, 152, 202, 334, 539, 631
LmnI GCTCC 3 cut(s) 91, 395, 668
LpnPI CCDG 3 cut(s) 384, 558, 717
Lsp1109I GCAGC 2 cut(s) 530, 751
LweI GCATC 3 cut(s) 418, 820, 927
MaeI CTAG 3 cut(s) 30, 411, 524
MaeII ACGT 1 cut(s) 939
MaeIII GTNAC 1 cut(s) 362
MalI GATC 6 cut(s) 109, 154, 204, 336, 541, 633
MboI GATC 6 cut(s) 107, 152, 202, 334, 539, 631
MboII GAAGA 3 cut(s) 32, 212, 311
MfeI CAATTG 3 cut(s) 213, 319, 663
MflI RGATCY 1 cut(s) 107
MluCI AATT 7 cut(s) 213, 319, 354, 528, 663, 675, 857
MmeI TCCRAC 1 cut(s) 240
MseI TTAA 4 cut(s) 450, 480, 758, 860
MslI CAYNNNNRTG 2 cut(s) 758, 841
MunI CAATTG 3 cut(s) 213, 319, 663
MwoI GCNNNNNNNGC 4 cut(s) 506, 515, 578, 668
NdeII GATC 6 cut(s) 107, 152, 202, 334, 539, 631
NlaIII CATG 6 cut(s) 236, 661, 757, 771, 840, 846
NlaIV GGNNCC 3 cut(s) 670, 751, 868
NmuCI GTSAC 1 cut(s) 362
NspI RCATGY 1 cut(s) 771
PaeI GCATGC 1 cut(s) 771
PceI AGGCCT 1 cut(s) 703
PfeI GAWTC 4 cut(s) 70, 551, 692, 784
PkrI GCNGC 2 cut(s) 520, 766
PsiI TTATAA 1 cut(s) 492
Psp1406I AACGTT 1 cut(s) 939
PspN4I GGNNCC 3 cut(s) 670, 751, 868
PspPI GGNCC 1 cut(s) 867
PsuI RGATCY 1 cut(s) 107
RsaI GTAC 3 cut(s) 724, 743, 911
RsaNI GTAC 3 cut(s) 723, 742, 910
RseI CAYNNNNRTG 2 cut(s) 758, 841
SaqAI TTAA 4 cut(s) 450, 480, 758, 860
SatI GCNGC 2 cut(s) 519, 765
Sau3AI GATC 6 cut(s) 107, 152, 202, 334, 539, 631
Sau96I GGNCC 1 cut(s) 867
ScaI AGTACT 1 cut(s) 743
SfaNI GCATC 3 cut(s) 418, 820, 927
SfcI CTRYAG 1 cut(s) 714
SmiMI CAYNNNNRTG 2 cut(s) 758, 841
SphI GCATGC 1 cut(s) 771
Sse9I AATT 7 cut(s) 213, 319, 354, 528, 663, 675, 857
SseBI AGGCCT 1 cut(s) 703
SsiI CCGC 1 cut(s) 11
SspI AATATT 1 cut(s) 466
SspMI CTAG 3 cut(s) 30, 411, 524
StuI AGGCCT 1 cut(s) 703
TaiI ACGT 1 cut(s) 942
TaqI TCGA 2 cut(s) 568, 595
TasI AATT 7 cut(s) 213, 319, 354, 528, 663, 675, 857
TatI WGTACW 1 cut(s) 741
TfiI GAWTC 4 cut(s) 70, 551, 692, 784
Tru1I TTAA 4 cut(s) 450, 480, 758, 860
Tru9I TTAA 4 cut(s) 450, 480, 758, 860
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 2 cut(s) 518, 764
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 3 cut(s) 33, 646, 886
TspGWI ACGGA 1 cut(s) 927
XceI RCATGY 1 cut(s) 771
XcmI CCANNNNNNNNNTGG 1 cut(s) 318
XmiI GTMKAC 1 cut(s) 816
XspI CTAG 3 cut(s) 30, 411, 524
ZrmI AGTACT 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.