Rorug04G0022000

MULE transposase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0013646
Physical Location & Seq
Reverse (-)
3160538 .. 3161122
585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0022000.1

Sequence Viewer

Length: 474 bp
ATGTCTAACTTTGGTCAAGTTGTTTCTGCAGGGATCCAGAGCGTCAATGATCCAGGTGGAGTCCTAGTTCGTCTAGGAAAATTTAATAGCGGTGCTTTTATTAATGCGGACATCTACGATGAGACTTTGGAATTGTTCGATCTTAGGGCCTTTGCTCGACGGCTTTTCAGGATTAGACGTGTTGGAATTGATCGTCCCATCACAGACGAAATCGATGCTAGGGTTGCAAGGGAATTAGCCGAGGAGGCATTATTAGGAAAGCCTAGCACTAAGCCTGCAACTAAATCAGCCATTGAGGGGTTGGAGAGAGTGAGAGTTGATAAGTTGTGTGGTATTTGTATGGACGACTTTGAGGATGGTCTAATAGCTACTCGCTTCCCATGCTTACACATTTTTCATGGACATTGCATTCACTGGTGGCTCGAGACCGATCACAAGTGTCCTTTGTGCCGATACCCATTGCTTTGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

157

Amino Acids

17.69

Weight (kDa)

5.95

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 106 - 151 2e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 109 - 151 1e-12 Ring finger domain
zf-C3HC4_2 PF13923 110 - 150 3.3e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 110 - 150 1.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 110 - 137 2.2e-06 RING-like zinc finger
zf-RING_UBOX PF13445 110 - 148 4.6e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 90, 107
AclWI GGATC 3 cut(s) 28, 41, 44
AcsI RAATTY 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 297
AflIII ACRYGT 1 cut(s) 178
AjiI CACGTC 1 cut(s) 179
AjnI CCWGG 1 cut(s) 52
AloI GAACNNNNNNTCC 2 cut(s) 51, 83
AluBI AGCT 1 cut(s) 368
AluI AGCT 1 cut(s) 368
Alw26I GTCTC 2 cut(s) 116, 419
AlwI GGATC 3 cut(s) 28, 41, 44
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 1 cut(s) 147
ApoI RAATTY 1 cut(s) 80
AseI ATTAAT 1 cut(s) 102
AspS9I GGNCC 1 cut(s) 147
AvaI CYCGRG 1 cut(s) 422
BamHI GGATCC 1 cut(s) 33
BccI CCATC 2 cut(s) 206, 350
BceAI ACGGC 1 cut(s) 176
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 1 cut(s) 54
BcoDI GTCTC 2 cut(s) 116, 419
BfaI CTAG 4 cut(s) 65, 74, 219, 264
BfmI CTRYAG 1 cut(s) 27
BglI GCCNNNNNGGC 1 cut(s) 245
Bme1390I CCNGG 1 cut(s) 54
BmeT110I CYCGRG 1 cut(s) 422
BmgBI CACGTC 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 1 cut(s) 35
BmrFI CCNGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 205
Bsa29I ATCGAT 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse1I ACTGG 1 cut(s) 419
Bse3DI GCAATG 2 cut(s) 403, 458
BseBI CCWGG 1 cut(s) 54
BseCI ATCGAT 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMI GCAATG 2 cut(s) 403, 458
BseNI ACTGG 1 cut(s) 419
BseRI GAGGAG 1 cut(s) 257
BshFI GGCC 1 cut(s) 149
BshVI ATCGAT 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 422
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 2 cut(s) 116, 419
BsmFI GGGAC 1 cut(s) 180
BsmI GAATGC 1 cut(s) 408
BsnI GGCC 1 cut(s) 149
Bso31I GGTCTC 1 cut(s) 419
BsoBI CYCGRG 1 cut(s) 422
Bsp143I GATC 5 cut(s) 33, 49, 139, 190, 430
BspACI CCGC 2 cut(s) 90, 107
BspANI GGCC 1 cut(s) 149
BspDI ATCGAT 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 35
BspMAI CTGCAG 1 cut(s) 31
BspPI GGATC 3 cut(s) 28, 41, 44
BspTNI GGTCTC 1 cut(s) 419
BsrDI GCAATG 2 cut(s) 403, 458
BsrI ACTGG 1 cut(s) 419
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 5 cut(s) 33, 49, 139, 190, 430
Bst2UI CCWGG 1 cut(s) 54
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 143, 270
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 5 cut(s) 36, 52, 142, 193, 433
BstMAI GTCTC 2 cut(s) 116, 419
BstMBI GATC 5 cut(s) 33, 49, 139, 190, 430
BstMWI GCNNNNNNNGC 3 cut(s) 224, 245, 381
BstNI CCWGG 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 52
BstSFI CTRYAG 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
Bsu15I ATCGAT 1 cut(s) 213
BsuRI GGCC 1 cut(s) 149
BsuTUI ATCGAT 1 cut(s) 213
BtrI CACGTC 1 cut(s) 179
BtsCI GGATG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 412
Cac8I GCNNGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 147
ClaI ATCGAT 1 cut(s) 213
CseI GACGC 1 cut(s) 31
CviAII CATG 2 cut(s) 381, 398
CviJI RGCY 8 cut(s) 149, 163, 239, 262, 274, 290, 368, 421
CviKI_1 RGCY 8 cut(s) 149, 163, 239, 262, 274, 290, 368, 421
DdeI CTNAG 2 cut(s) 143, 270
DpnI GATC 5 cut(s) 35, 51, 141, 192, 432
DpnII GATC 5 cut(s) 33, 49, 139, 190, 430
Eco31I GGTCTC 1 cut(s) 419
Eco88I CYCGRG 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 52
FaeI CATG 2 cut(s) 384, 401
FaiI YATR 3 cut(s) 341, 382, 399
FaqI GGGAC 1 cut(s) 180
FatI CATG 2 cut(s) 380, 397
FokI GGATG 1 cut(s) 368
FspBI CTAG 4 cut(s) 65, 74, 219, 264
HaeIII GGCC 1 cut(s) 149
HgaI GACGC 1 cut(s) 31
Hin1II CATG 2 cut(s) 384, 401
HinfI GANTC 1 cut(s) 60
Hpy188III TCNNGA 3 cut(s) 37, 169, 424
Hpy99I CGWCG 1 cut(s) 162
HpyCH4IV ACGT 1 cut(s) 178
HpyCH4V TGCA 4 cut(s) 29, 227, 278, 408
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 245, 381
HpyF3I CTNAG 2 cut(s) 143, 270
HpySE526I ACGT 1 cut(s) 178
Hsp92II CATG 2 cut(s) 384, 401
Kzo9I GATC 5 cut(s) 33, 49, 139, 190, 430
LpnPI CCDG 7 cut(s) 15, 39, 50, 66, 154, 288, 400
LweI GCATC 1 cut(s) 205
MaeI CTAG 4 cut(s) 65, 74, 219, 264
MaeII ACGT 1 cut(s) 178
MalI GATC 5 cut(s) 35, 51, 141, 192, 432
MboI GATC 5 cut(s) 33, 49, 139, 190, 430
MflI RGATCY 1 cut(s) 33
MluCI AATT 4 cut(s) 80, 131, 186, 233
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 2 cut(s) 163, 282
MnlI CCTC 4 cut(s) 235, 238, 289, 346
MseI TTAA 2 cut(s) 84, 102
MspR9I CCNGG 1 cut(s) 54
Mva1269I GAATGC 1 cut(s) 408
MvaI CCWGG 1 cut(s) 54
MwoI GCNNNNNNNGC 3 cut(s) 224, 245, 381
NdeII GATC 5 cut(s) 33, 49, 139, 190, 430
NlaIII CATG 2 cut(s) 384, 401
NlaIV GGNNCC 1 cut(s) 35
NmeAIII GCCGAG 1 cut(s) 265
PaeR7I CTCGAG 1 cut(s) 422
PctI GAATGC 1 cut(s) 408
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PshBI ATTAAT 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 35
PspPI GGNCC 1 cut(s) 147
PstI CTGCAG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 33
SaqAI TTAA 2 cut(s) 84, 102
Sau3AI GATC 5 cut(s) 33, 49, 139, 190, 430
Sau96I GGNCC 1 cut(s) 147
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 1 cut(s) 54
SetI ASST 3 cut(s) 58, 181, 370
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 1 cut(s) 27
Sfr274I CTCGAG 1 cut(s) 422
SlaI CTCGAG 1 cut(s) 422
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 4 cut(s) 80, 131, 186, 233
SsiI CCGC 2 cut(s) 90, 107
SspMI CTAG 4 cut(s) 65, 74, 219, 264
StyD4I CCNGG 1 cut(s) 52
TaiI ACGT 1 cut(s) 181
TaqI TCGA 4 cut(s) 138, 157, 213, 423
TaqII GACCGA 1 cut(s) 443
TasI AATT 4 cut(s) 80, 131, 186, 233
Tru1I TTAA 2 cut(s) 84, 102
Tru9I TTAA 2 cut(s) 84, 102
TscAI CASTG 1 cut(s) 419
TspDTI ATGAA 1 cut(s) 386
TspRI CASTG 1 cut(s) 419
VspI ATTAAT 1 cut(s) 102
XapI RAATTY 1 cut(s) 80
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 422
XspI CTAG 4 cut(s) 65, 74, 219, 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.