Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0005533

Physical Location & Seq

Genomic Coordinates Forward (+)

31562615 .. 31566779

Gene Structure 4165 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g36850.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3126 bp

ATGGTTATCCTTCTTCTTCCTCACCGATCCTCGAAAACAATGGCTTCCCTCTTCCATACTCTACTGTCTTCTTCGTTTTCCATGTTAGCTCTTCTTCTATGCATATGTCTGAGCGTAGAGTCTGCTATAATTCCAAGCATTACCACTGACAAGGAGGCTTTGATCTCATTCAAGGAGGCCTTGCTCAGTGCCCCTCCATCTTTAAGCTGGGACCAGAATTCATCACCTTGCAGCAACTGGACTGGAGTTGTTTGCAGAAACGATGGTCAGAGTCCGAGAGTTGTTGGTCTTGATCTTTCAAGCTTGGGACTTACAGGGACCATAAGCCCTCAGATTGGTAACTTATCCTTCCTCCGTTCCCTTCAACTTCAAAACAACAAGTTAACAGGAGCAATTCCTACTCAACTTGTTAACCTTTACCGCTTGAGATCTCTGAACCTGAGCTCCAACACCATACAAGGTCCACTACCTTCAAACATGAGCAACTTGAATGCACTAGAAGTCCTCGACTTGGCTGGGAACAACATAACAGGGAGGCTCCCTGAGGAGATGTTCAGCCAAAAGAAACTTCAAGTCTTGAATCTGGCACGAAATAAATTCTTTGGTTCTGTTCCTTCATCCATAAGCAACCTTTCATCCACACTCACATCTCTGAACTTGGGTACAAATAGCCTCAGTGGGATCATACCTAGTGAGTTGGGTCTACTTAACAATCTCAAGGAACTTGATCTTTCTGGTAACAAGTTCACTGGGACTGTTGCTCCCTCCATATACAACATTACTTCATTGGTTCTGTTTACTGTTGCTTCCAATCAACTTTGGGGTGAGATTCCCAAAGATATTGATCAGACACTTCCAAATCTTTTATACTACCGGAACTGCTTCAATCTAATGACAGGGAACATTCCTGCTTCTTTGCACAACATCACCAAGATTCGATCAATTCGAATGTCAAACAATTTTCTGGAAGGAACAGTTCCACCAGGGCTTGGAAACATGCCAGACCTTGAAATGTACAACATTGGCTTCAATCGGATCGTTAGTAAAGGCAGTGATGGTCTCAGTTTTATCACTTCATTGACAAACAGCACCAACCTGCAGTTTCTTGCTTTCGACGACAATCAGTTAGAGGGAGTCATTCCAGAATCACTTGGCAATCTCTCGAAGGTTCTCAACAAATTGTACATGGGAGGGAATCGGATTTCCGGTAATATTCCTGCCTCAGTTGGTCGTCTTACTAGTTTAACTTTGTTAAATGTGAGCTACAATTCAATATCTGGTGAAATTCCTACTGAAATAGGCCAATTGGAAAACCTACAAGAGCTTAGTGTAGCAGGAAATGATTTGTCTGGTCATATTCCAAACTCCTTGGGAAATCTCAAAAAGTTGAACAGCATTGATTTATCTGGGAATCACTTTGTTGGCCAAATACCCTCTTCTTTTTCAAACTTCCAGAATCTGCTTTCCATGGACTTATCAAACAATGAGCTCAACGGAACCATTTCCGGTGAAACTCTCAACCTCCCTAGTTTAAGCACCACTCTCAATCTGTCCCAAAACTTTCTAAGTGGACCTTTGCCTTCAGAACTTGGGTCCCTAGAGAAGGTTGTTACTATAGACCTCTCTGACAATGCTTTATCTGGAGATATCCCGGGATCAATAGGAAAGTGTAAAAGCTTGGAGAGATTGTTGATGTCAAGAAATAGATTTTCAGGTTCTATTCCCAATGGTGTAGGAGAATTGAGAGGCCTTGAGTTTCTAGACCTTTCCTCAAACCAGCTTTCCAGCTCCATTCCAGAGAATTTTCAAGACCTACATGCCCTTCAGTACTTGAACCTATCATTTAATCACTTAGAAGGAGCGATTCCTAATGGTGGATTATTTGTTAAAAATTTCACTAATGTTCATCTTGAAGGCAACCCAGAAATTTGCCTTAAATTTCCATGTGTGAAGAATAGTAATGGTCGTCGAAGCAGAAAAATACTAGTCCCAGTTGTGATCATTACAACTGTCTTAGCAACCATAGCAATATGTGTTATAGTGGGCTGTTTGGTCTATGCTAAGAAAAGAAAAGGTTGCAAAACAAAAATTACAGGAACTTGTGATGACTTCGTGGTAAAAGGGCAGCATCAAATGGTTTCGTATGAAGAACTCCGTGGCTCAACAGGGAATTTCAACCCAGGAAATTTGGTTGGAAGGGGGAGCTTTGGTTCTGTATATAAAGGGTTTCTTAGAGACCAAGGAATTGAAGTTGCAGTCAAGGTGCTTGATATAAGACCAAAAGGGTCTTGGAAGAGTTTCTTGGCAGAGTGTAATGCTTTAAGGAGTGTGAGGCACCGGAATCTTGTCAAACTGATTACTTCCTGCTCAAGCTTAGACTATAAGAACATGGAGTTTCTGGCTTTGGTGTATGAGTATCTGAGTAATGGGAGCTTGGAAGATTGGATCAGAGGCAAGAGAACAAATTCAGACGGAAGCGGTTTAAGCATTGTGGAGAGATTGAATGTGGTGATTGATGTTGCTTGTGGATTGGATTACCTCCACAATGATTGTGAAGTTCCAGTGGTTCACTGTGATTTGAAGCCAAGCAACATTCTTATGGACAAAGACATGACTGCAAAGATTGGAGATTTTGGGCTGGCAAAGTTGCTGATAGAGGAAACATCCACTCAACATTCTATTGGCTCCACCAATGTCCTCAAGGGTTCCATTGGATACATACCCCCAGAATATGGTTTTGGACAAAAACCCTCCACAGCTGGAGATGCATACAGTTTTGGAGTAATGTTGTTGGAGCTGTTTACTGGAAAAAGTCCAACCGATGAGCGCTTCACAGGAGAAGTTAACCTCGTTCAATGGGTGCAGTCATCATTTCCTCACAACATTGCAGTAGTACTAGACTCTGAGTTGTTACATTGTCAGCACGACGAAGGCTCAAATCTCATCTCAGAAGAAGAGCTGCATTGTCTGAACTCAGTTATCGAGGTTGGGCTCTCTTGCGCTTTCACCTCTCCTGATGGACGCATTAGCTTGAGGGATGCTCTTCACAAACTTCAAACTGCTAGAGAGACTTTTTTGAAGCTTGCTCACTTTGACAGTCCAGAGTGCAACTTTCAACAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009987 GO:0010204 GO:0010359 GO:0012501 GO:0012505 GO:0016020 GO:0016045 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031347 GO:0031349 GO:0032409 GO:0032412 GO:0032879 GO:0033554 GO:0034050 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080134 GO:0098543 GO:0098581 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1042

Amino Acids

113.07

Weight (kDa)

5.94

Isoelectric Point (pI)

37.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	49 - 85	4.1e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	108 - 246	3.4e-14	Leucine-rich repeat region
LRR_8	PF13855	118 - 176	3.6e-09	Leucine rich repeat
LRR_8	PF13855	189 - 249	1.9e-06	Leucine rich repeat
Pkinase	PF00069	432 - 647	9.6e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	433 - 647	3.1e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g36850 FvH4_3g36850 FvH4_3g36850
malus_domestica	MD08G1090900.v1.1 MD15G1074900.v1.1 MD15G1075000.v1.1
prunus_persica	Prupe.1G427400_v2.0.a1 Prupe.1G427700_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1
pyrus_communis	pycom15g06980 pycom15g06990
rosa_chinensis	RchiOBHm_Chr5g0066001 RchiOBHm_Chr5g0066021
rosa_laevigata	RLG00000035833 RLG00000035834
rosa_multiflora	Rmu_sc0001473.1_g000006 Rmu_sc0011576.1_g000012 Rmu_sc0011576.1_g000015
rosa_roxburghii	Rroxscaffold_1G00014980 Rroxscaffold_1G00015120
rosa_rugosa	Rorug05G0375800 Rorug05G0375900 Rorug05G0376000
rosa_samantha	Rh5AG434300 Rh5AG434500 Rh5CG472900
rosa_wichuraiana	Rw5G040690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1104
AccB1I	GGYRCC	1 cut(s)	2334
AccB7I	CCANNNNNTGG	2 cut(s)	989, 2732
AccI	GTMKAC	1 cut(s)	703
AciI	CCGC	2 cut(s)	421, 2478
AclWI	GGATC	5 cut(s)	21, 689, 1043, 1663, 2453
AcoI	YGGCCR	1 cut(s)	1423
AcuI	CTGAAG	2 cut(s)	1566, 1808
AfaI	GTAC	5 cut(s)	664, 1016, 1184, 1829, 2895
AfeI	AGCGCT	1 cut(s)	2828
AfiI	CCNNNNNNNGG	6 cut(s)	335, 511, 989, 1603, 1874, 2732
AhlI	ACTAGT	2 cut(s)	1238, 1984
AjnI	CCWGG	2 cut(s)	982, 2179
AjuI	GAANNNNNNNTTGG	6 cut(s)	552, 584, 2285, 2317, 2721, 2753
AloI	GAACNNNNNNTCC	4 cut(s)	428, 460, 2194, 2226
Alw21I	GWGCWC	2 cut(s)	446, 1491
Alw26I	GTCTC	3 cut(s)	1064, 2229, 3062
AlwI	GGATC	5 cut(s)	21, 689, 1043, 1663, 2453
AlwNI	CAGNNNCTG	2 cut(s)	237, 1459
Ama87I	CYCGRG	1 cut(s)	1651
Aor51HI	AGCGCT	1 cut(s)	2828
AoxI	GGCC	4 cut(s)	177, 1300, 1423, 1747
ApeKI	GCWGC	3 cut(s)	231, 2125, 2959
Asp700I	GAANNNNTTC	4 cut(s)	881, 1501, 2297, 2464
AspLEI	GCGC	2 cut(s)	2829, 3002
AspS9I	GGNCC	5 cut(s)	211, 318, 461, 1571, 1593
AsuC2I	CCSGG	2 cut(s)	1652, 1653
AsuHPI	GGTGA	8 cut(s)	14, 216, 836, 919, 1292, 1520, 2521, 2998
AsuII	TTCGAA	1 cut(s)	946
AvaI	CYCGRG	1 cut(s)	1651
AvaII	GGWCC	5 cut(s)	211, 318, 461, 1571, 1593
AxyI	CCTNAGG	1 cut(s)	543
BaeGI	GKGCMC	1 cut(s)	193
BalI	TGGCCA	1 cut(s)	1425
BanI	GGYRCC	1 cut(s)	2334
BanII	GRGCYC	3 cut(s)	446, 1491, 2994
BbsI	GAAGAC	1 cut(s)	60
Bbv12I	GWGCWC	2 cut(s)	446, 1491
BbvI	GCAGC	3 cut(s)	243, 2137, 2946
BccI	CCATC	4 cut(s)	205, 257, 1049, 3011
BciT130I	CCWGG	2 cut(s)	984, 2181
BciVI	GTATCC	1 cut(s)	2708
BclI	TGATCA	2 cut(s)	844, 1998
BcnI	CCSGG	2 cut(s)	1652, 1653
BcoDI	GTCTC	3 cut(s)	1064, 2229, 3062
BcuI	ACTAGT	2 cut(s)	1238, 1984
BfaI	CTAG	9 cut(s)	497, 690, 1239, 1527, 1598, 1760, 1985, 2897, 3063
BfmI	CTRYAG	2 cut(s)	1097, 1614
BfoI	RGCGCY	1 cut(s)	2830
BfuAI	ACCTGC	1 cut(s)	1104
BfuI	GTATCC	1 cut(s)	2708
BglII	AGATCT	1 cut(s)	428
BisI	GCNGC	3 cut(s)	232, 2126, 2960
BlsI	GCNGC	3 cut(s)	233, 2127, 2961
BmcAI	AGTACT	2 cut(s)	1829, 2895
Bme1390I	CCNGG	4 cut(s)	984, 1652, 1653, 2181
Bme18I	GGWCC	5 cut(s)	211, 318, 461, 1571, 1593
BmeT110I	CYCGRG	1 cut(s)	1651
BmgT120I	GGNCC	5 cut(s)	211, 318, 461, 1571, 1593
BmiI	GGNNCC	9 cut(s)	212, 319, 539, 1498, 1594, 1595, 2336, 2686, 2707
BmrFI	CCNGG	4 cut(s)	984, 1652, 1653, 2181
BmrI	ACTGGG	2 cut(s)	759, 1985
BmsI	GCATC	3 cut(s)	2137, 2755, 3028
BmuI	ACTGGG	2 cut(s)	759, 1985
BpiI	GAAGAC	1 cut(s)	60
BplI	GAGNNNNNCTC	2 cut(s)	3025, 3057
BpmI	CTGGAG	3 cut(s)	264, 1662, 2781
Bpu10I	CCTNAGC	1 cut(s)	440
Bpu14I	TTCGAA	1 cut(s)	946
BpuEI	CTTGAG	6 cut(s)	445, 701, 1772, 2353, 2684, 3052
BpuMI	CCSGG	2 cut(s)	1652, 1653
BsaBI	GATNNNNATC	1 cut(s)	843
BsaI	GGTCTC	2 cut(s)	1064, 2229
BsaJI	CCNNGG	7 cut(s)	983, 1368, 1467, 1651, 2155, 2179, 2239
BsaWI	WCCGGW	4 cut(s)	873, 1205, 1505, 2337
BsaXI	ACNNNNNCTCC	8 cut(s)	428, 458, 745, 775, 1673, 1703, 2194, 2224
Bsc4I	CCNNNNNNNGG	6 cut(s)	335, 511, 989, 1603, 1874, 2732
Bse1I	ACTGG	6 cut(s)	242, 247, 754, 1991, 2561, 2809
Bse21I	CCTNAGG	1 cut(s)	543
Bse3DI	GCAATG	1 cut(s)	2883
Bse8I	GATNNNNATC	1 cut(s)	843
BseBI	CCWGG	2 cut(s)	984, 2181
BseDI	CCNNGG	7 cut(s)	983, 1368, 1467, 1651, 2155, 2179, 2239
BseGI	GGATG	4 cut(s)	617, 635, 2663, 3043
BseJI	GATNNNNATC	1 cut(s)	843
BseLI	CCNNNNNNNGG	6 cut(s)	335, 511, 989, 1603, 1874, 2732
BseMI	GCAATG	1 cut(s)	2883
BseNI	ACTGG	6 cut(s)	242, 247, 754, 1991, 2561, 2809
BseRI	GAGGAG	1 cut(s)	560
BseSI	GKGCMC	1 cut(s)	193
BseXI	GCAGC	3 cut(s)	243, 2137, 2946
BseYI	CCCAGC	2 cut(s)	207, 515
BsgI	GTGCAG	1 cut(s)	2882
BshFI	GGCC	4 cut(s)	179, 1302, 1425, 1749
BshNI	GGYRCC	1 cut(s)	2334
BsiHKAI	GWGCWC	2 cut(s)	446, 1491
BsiHKCI	CYCGRG	1 cut(s)	1651
BsiSI	CCGG	5 cut(s)	874, 1206, 1506, 1652, 2338
BslFI	GGGAC	7 cut(s)	224, 321, 331, 766, 1537, 1579, 1973
BslI	CCNNNNNNNGG	6 cut(s)	335, 511, 989, 1603, 1874, 2732
BsmAI	GTCTC	3 cut(s)	1064, 2229, 3062
BsmFI	GGGAC	7 cut(s)	224, 321, 331, 766, 1537, 1579, 1973
BsmI	GAATGC	1 cut(s)	496
BsnI	GGCC	4 cut(s)	179, 1302, 1425, 1749
Bso31I	GGTCTC	2 cut(s)	1064, 2229
BsoBI	CYCGRG	1 cut(s)	1651
Bsp119I	TTCGAA	1 cut(s)	946
Bsp1286I	GDGCHC	4 cut(s)	193, 446, 1491, 2994
Bsp1407I	TGTACA	2 cut(s)	1014, 1182
Bsp19I	CCATGG	1 cut(s)	1467
BspACI	CCGC	2 cut(s)	421, 2478
BspANI	GGCC	4 cut(s)	179, 1302, 1425, 1749
BspLI	GGNNCC	9 cut(s)	212, 319, 539, 1498, 1594, 1595, 2336, 2686, 2707
BspMAI	CTGCAG	1 cut(s)	1101
BspMI	ACCTGC	1 cut(s)	1104
BspPI	GGATC	5 cut(s)	21, 689, 1043, 1663, 2453
BspQI	GCTCTTC	3 cut(s)	96, 2949, 3048
BspT104I	TTCGAA	1 cut(s)	946
BspT107I	GGYRCC	1 cut(s)	2334
BspTNI	GGTCTC	2 cut(s)	1064, 2229
BsrDI	GCAATG	1 cut(s)	2883
BsrGI	TGTACA	2 cut(s)	1014, 1182
BsrI	ACTGG	6 cut(s)	242, 247, 754, 1991, 2561, 2809
BssECI	CCNNGG	7 cut(s)	983, 1368, 1467, 1651, 2155, 2179, 2239
BssT1I	CCWWGG	3 cut(s)	1368, 1467, 2239
Bst2UI	CCWGG	2 cut(s)	984, 2181
Bst4CI	ACNGT	8 cut(s)	66, 757, 802, 976, 2011, 2573, 2774, 3098
Bst6I	CTCTTC	6 cut(s)	56, 96, 1441, 2288, 2949, 3048
BstAUI	TGTACA	2 cut(s)	1014, 1182
BstBI	TTCGAA	1 cut(s)	946
BstC8I	GCNNGC	2 cut(s)	2640, 3084
BstDSI	CCRYGG	2 cut(s)	1467, 2155
BstENI	CCTNNNNNAGG	1 cut(s)	1601
BstF5I	GGATG	4 cut(s)	617, 635, 2663, 3043
BstH2I	RGCGCY	1 cut(s)	2830
BstHHI	GCGC	2 cut(s)	2829, 3002
BstMAI	GTCTC	3 cut(s)	1064, 2229, 3062
BstMWI	GCNNNNNNNGC	3 cut(s)	2024, 2484, 2765
BstNI	CCWGG	2 cut(s)	984, 2181
BstNSI	RCATGY	2 cut(s)	1000, 1820
BstSCI	CCNGG	4 cut(s)	982, 1650, 1651, 2179
BstSFI	CTRYAG	2 cut(s)	1097, 1614
BstSLI	GKGCMC	1 cut(s)	193
BstV1I	GCAGC	3 cut(s)	243, 2137, 2946
BstV2I	GAAGAC	1 cut(s)	60
BstX2I	RGATCY	1 cut(s)	428
BstYI	RGATCY	1 cut(s)	428
Bsu36I	CCTNAGG	1 cut(s)	543
BsuI	GTATCC	1 cut(s)	2708
BsuRI	GGCC	4 cut(s)	179, 1302, 1425, 1749
BtgI	CCRYGG	2 cut(s)	1467, 2155
BtsCI	GGATG	4 cut(s)	617, 635, 2663, 3043
BtsI	GCAGTG	1 cut(s)	1057
BtsIMutI	CAGTG	7 cut(s)	144, 193, 682, 747, 1057, 2568, 2569
BveI	ACCTGC	1 cut(s)	1104
Cac8I	GCNNGC	2 cut(s)	2640, 3084
CaiI	CAGNNNCTG	2 cut(s)	237, 1459
CfoI	GCGC	2 cut(s)	2829, 3002
Cfr13I	GGNCC	5 cut(s)	211, 318, 461, 1571, 1593
Cfr9I	CCCGGG	1 cut(s)	1651
CseI	GACGC	1 cut(s)	3030
Csp6I	GTAC	5 cut(s)	663, 1015, 1183, 1828, 2894
CspCI	CAANNNNNGTGG	2 cut(s)	2531, 2566
CviAII	CATG	9 cut(s)	82, 478, 997, 1186, 1468, 1817, 1944, 2389, 2611
CviQI	GTAC	5 cut(s)	663, 1015, 1183, 1828, 2894
EaeI	YGGCCR	1 cut(s)	1423
Eam1104I	CTCTTC	6 cut(s)	56, 96, 1441, 2288, 2949, 3048
EarI	CTCTTC	6 cut(s)	56, 96, 1441, 2288, 2949, 3048
Ecl136II	GAGCTC	2 cut(s)	444, 1489
Eco130I	CCWWGG	3 cut(s)	1368, 1467, 2239
Eco147I	AGGCCT	2 cut(s)	179, 1749
Eco24I	GRGCYC	3 cut(s)	446, 1491, 2994
Eco31I	GGTCTC	2 cut(s)	1064, 2229
Eco32I	GATATC	1 cut(s)	1648
Eco47I	GGWCC	5 cut(s)	211, 318, 461, 1571, 1593
Eco47III	AGCGCT	1 cut(s)	2828
Eco53kI	GAGCTC	2 cut(s)	444, 1489
Eco57I	CTGAAG	2 cut(s)	1566, 1808
Eco81I	CCTNAGG	1 cut(s)	543
Eco88I	CYCGRG	1 cut(s)	1651
EcoICRI	GAGCTC	2 cut(s)	444, 1489
EcoNI	CCTNNNNNAGG	1 cut(s)	1601
EcoO109I	RGGNCCY	1 cut(s)	1593
EcoRI	GAATTC	1 cut(s)	217
EcoRII	CCWGG	2 cut(s)	982, 2179
EcoRV	GATATC	1 cut(s)	1648
EcoT14I	CCWWGG	3 cut(s)	1368, 1467, 2239
EcoT22I	ATGCAT	2 cut(s)	104, 2770
EcoT38I	GRGCYC	3 cut(s)	446, 1491, 2994
ErhI	CCWWGG	3 cut(s)	1368, 1467, 2239
FaeI	CATG	9 cut(s)	85, 481, 1000, 1189, 1471, 1820, 1947, 2392, 2614
FalI	AAGNNNNNCTT	8 cut(s)	164, 196, 1558, 1590, 2214, 2246, 2285, 2317
FaqI	GGGAC	7 cut(s)	224, 321, 331, 766, 1537, 1579, 1973
FatI	CATG	9 cut(s)	81, 477, 996, 1185, 1467, 1816, 1943, 2388, 2610
FauNDI	CATATG	1 cut(s)	104
FbaI	TGATCA	2 cut(s)	844, 1998
FblI	GTMKAC	1 cut(s)	703
Fnu4HI	GCNGC	3 cut(s)	232, 2126, 2960
FokI	GGATG	4 cut(s)	604, 622, 2650, 3050
FriOI	GRGCYC	3 cut(s)	446, 1491, 2994
Fsp4HI	GCNGC	3 cut(s)	232, 2126, 2960
FspBI	CTAG	9 cut(s)	497, 690, 1239, 1527, 1598, 1760, 1985, 2897, 3063
GlaI	GCGC	2 cut(s)	2828, 3001
GluI	GCNGC	3 cut(s)	232, 2126, 2960
GsaI	CCCAGC	2 cut(s)	211, 519
GsuI	CTGGAG	3 cut(s)	264, 1662, 2781
HaeII	RGCGCY	1 cut(s)	2830
HaeIII	GGCC	4 cut(s)	179, 1302, 1425, 1749
HapII	CCGG	5 cut(s)	874, 1206, 1506, 1652, 2338
HgaI	GACGC	1 cut(s)	3030
HhaI	GCGC	2 cut(s)	2829, 3002
Hin1II	CATG	9 cut(s)	85, 481, 1000, 1189, 1471, 1820, 1947, 2392, 2614
Hin6I	GCGC	2 cut(s)	2827, 3000
HinP1I	GCGC	2 cut(s)	2827, 3000
HincII	GTYRAC	3 cut(s)	384, 412, 2845
HindII	GTYRAC	3 cut(s)	384, 412, 2845
HindIII	AAGCTT	4 cut(s)	301, 1675, 2371, 3080
HpaI	GTTAAC	3 cut(s)	384, 412, 2845
HpaII	CCGG	5 cut(s)	874, 1206, 1506, 1652, 2338
HphI	GGTGA	8 cut(s)	14, 216, 836, 919, 1292, 1520, 2521, 2998
Hpy99I	CGWCG	3 cut(s)	1118, 1971, 2930
HpyCH4III	ACNGT	8 cut(s)	66, 757, 802, 976, 2011, 2573, 2774, 3098
HpyF10VI	GCNNNNNNNGC	3 cut(s)	2024, 2484, 2765
Hsp92II	CATG	9 cut(s)	85, 481, 1000, 1189, 1471, 1820, 1947, 2392, 2614
HspAI	GCGC	2 cut(s)	2827, 3000
KflI	GGGWCCC	1 cut(s)	1593
Ksp22I	TGATCA	2 cut(s)	844, 1998
KspAI	GTTAAC	3 cut(s)	384, 412, 2845
LguI	GCTCTTC	3 cut(s)	96, 2949, 3048
Lsp1109I	GCAGC	3 cut(s)	243, 2137, 2946
LweI	GCATC	3 cut(s)	2137, 2755, 3028
MaeI	CTAG	9 cut(s)	497, 690, 1239, 1527, 1598, 1760, 1985, 2897, 3063
MaeIII	GTNAC	4 cut(s)	338, 737, 1609, 2910
MfeI	CAATTG	1 cut(s)	1304
MflI	RGATCY	1 cut(s)	428
MhlI	GDGCHC	4 cut(s)	193, 446, 1491, 2994
MlsI	TGGCCA	1 cut(s)	1425
MluNI	TGGCCA	1 cut(s)	1425
MlyI	GAGTC	4 cut(s)	128, 280, 1143, 2894
MmeI	TCCRAC	4 cut(s)	471, 2173, 2772, 2840
Mox20I	TGGCCA	1 cut(s)	1425
Mph1103I	ATGCAT	2 cut(s)	104, 2770
MroXI	GAANNNNTTC	4 cut(s)	881, 1501, 2297, 2464
MscI	TGGCCA	1 cut(s)	1425
MslI	CAYNNNNRTG	1 cut(s)	2597
Msp20I	TGGCCA	1 cut(s)	1425
MspA1I	CMGCKG	1 cut(s)	2759
MspI	CCGG	5 cut(s)	874, 1206, 1506, 1652, 2338
MspR9I	CCNGG	4 cut(s)	984, 1652, 1653, 2181
MunI	CAATTG	1 cut(s)	1304
Mva1269I	GAATGC	1 cut(s)	496
MvaI	CCWGG	2 cut(s)	984, 2181
MwoI	GCNNNNNNNGC	3 cut(s)	2024, 2484, 2765
NciI	CCSGG	2 cut(s)	1652, 1653
NcoI	CCATGG	1 cut(s)	1467
NdeI	CATATG	1 cut(s)	104
NlaIII	CATG	9 cut(s)	85, 481, 1000, 1189, 1471, 1820, 1947, 2392, 2614
NlaIV	GGNNCC	9 cut(s)	212, 319, 539, 1498, 1594, 1595, 2336, 2686, 2707
NsiI	ATGCAT	2 cut(s)	104, 2770
NspI	RCATGY	2 cut(s)	1000, 1820
NspV	TTCGAA	1 cut(s)	946
PceI	AGGCCT	2 cut(s)	179, 1749
PciSI	GCTCTTC	3 cut(s)	96, 2949, 3048
PcsI	WCGNNNNNNNCGW	1 cut(s)	943
PctI	GAATGC	1 cut(s)	496
PdmI	GAANNNNTTC	4 cut(s)	881, 1501, 2297, 2464
PfeI	GAWTC	9 cut(s)	580, 829, 934, 1145, 1195, 1411, 1456, 1864, 2341
PflMI	CCANNNNNTGG	2 cut(s)	989, 2732
PkrI	GCNGC	3 cut(s)	233, 2127, 2961
PleI	GAGTC	4 cut(s)	127, 279, 1142, 2894
PpsI	GAGTC	4 cut(s)	127, 279, 1142, 2894
PpuMI	RGGWCCY	1 cut(s)	1593
Psp124BI	GAGCTC	2 cut(s)	446, 1491
Psp5II	RGGWCCY	1 cut(s)	1593
Psp6I	CCWGG	2 cut(s)	982, 2179
PspFI	CCCAGC	2 cut(s)	207, 515
PspGI	CCWGG	2 cut(s)	982, 2179
PspN4I	GGNNCC	9 cut(s)	212, 319, 539, 1498, 1594, 1595, 2336, 2686, 2707
PspPI	GGNCC	5 cut(s)	211, 318, 461, 1571, 1593
PspPPI	RGGWCCY	1 cut(s)	1593
PstI	CTGCAG	1 cut(s)	1101
PstNI	CAGNNNCTG	2 cut(s)	237, 1459
PsuI	RGATCY	1 cut(s)	428
PvuII	CAGCTG	1 cut(s)	2759
RsaI	GTAC	5 cut(s)	664, 1016, 1184, 1829, 2895
RsaNI	GTAC	5 cut(s)	663, 1015, 1183, 1828, 2894
RseI	CAYNNNNRTG	1 cut(s)	2597
SacI	GAGCTC	2 cut(s)	446, 1491
SapI	GCTCTTC	3 cut(s)	96, 2949, 3048
SatI	GCNGC	3 cut(s)	232, 2126, 2960
Sau96I	GGNCC	5 cut(s)	211, 318, 461, 1571, 1593
ScaI	AGTACT	2 cut(s)	1829, 2895
SchI	GAGTC	4 cut(s)	128, 280, 1143, 2894
ScrFI	CCNGG	4 cut(s)	984, 1652, 1653, 2181
SduI	GDGCHC	4 cut(s)	193, 446, 1491, 2994
SfaNI	GCATC	3 cut(s)	2137, 2755, 3028
SfcI	CTRYAG	2 cut(s)	1097, 1614
SfuI	TTCGAA	1 cut(s)	946
SinI	GGWCC	5 cut(s)	211, 318, 461, 1571, 1593
SmaI	CCCGGG	1 cut(s)	1653
SmiMI	CAYNNNNRTG	1 cut(s)	2597
SmlI	CTYRAG	6 cut(s)	424, 716, 1751, 2368, 2699, 3031
SmoI	CTYRAG	6 cut(s)	424, 716, 1751, 2368, 2699, 3031
SpeI	ACTAGT	2 cut(s)	1238, 1984
SseBI	AGGCCT	2 cut(s)	179, 1749
SsiI	CCGC	2 cut(s)	421, 2478
SspI	AATATT	1 cut(s)	1213
SspMI	CTAG	9 cut(s)	497, 690, 1239, 1527, 1598, 1760, 1985, 2897, 3063
SstI	GAGCTC	2 cut(s)	446, 1491
StuI	AGGCCT	2 cut(s)	179, 1749
StyD4I	CCNGG	4 cut(s)	982, 1650, 1651, 2179
StyI	CCWWGG	3 cut(s)	1368, 1467, 2239
TaaI	ACNGT	8 cut(s)	66, 757, 802, 976, 2011, 2573, 2774, 3098
TaqI	TCGA	8 cut(s)	32, 507, 937, 946, 1113, 1163, 1969, 2982
TatI	WGTACW	4 cut(s)	1014, 1182, 1827, 2893
TfiI	GAWTC	9 cut(s)	580, 829, 934, 1145, 1195, 1411, 1456, 1864, 2341
TscAI	CASTG	7 cut(s)	151, 193, 682, 754, 1057, 2568, 2576
TseI	GCWGC	3 cut(s)	231, 2125, 2959
TspDTI	ATGAA	7 cut(s)	210, 606, 624, 774, 1065, 1895, 2160
TspGWI	ACGGA	4 cut(s)	344, 1509, 2144, 2487
TspMI	CCCGGG	1 cut(s)	1651
TspRI	CASTG	7 cut(s)	151, 193, 682, 754, 1057, 2568, 2576
Van91I	CCANNNNNTGG	2 cut(s)	989, 2732
VpaK11BI	GGWCC	5 cut(s)	211, 318, 461, 1571, 1593
XagI	CCTNNNNNAGG	1 cut(s)	1601
XbaI	TCTAGA	1 cut(s)	1759
XceI	RCATGY	2 cut(s)	1000, 1820
XcmI	CCANNNNNNNNNTGG	2 cut(s)	204, 2286
XmaI	CCCGGG	1 cut(s)	1651
XmiI	GTMKAC	1 cut(s)	703
XmnI	GAANNNNTTC	4 cut(s)	881, 1501, 2297, 2464
XspI	CTAG	9 cut(s)	497, 690, 1239, 1527, 1598, 1760, 1985, 2897, 3063
ZrmI	AGTACT	2 cut(s)	1829, 2895
Zsp2I	ATGCAT	2 cut(s)	104, 2770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.