Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005533

Physical Location & Seq

Genomic Coordinates Forward (+)

72078528 .. 72086288

Gene Structure 7761 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ34180

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3096 bp

ATGGATTCCCACTTCCATACTCTACTGCCTCCTCAGTTTTCCATGTTAGCTCTTCTTCTATGCATATCATTTCTGAGCGCAGAGTCTGCTATAATTCCAAGCATTACCACCGACAAGGAGGCCTTGATCTCATTCAAGGAGGCCTTGCTCGATGTCCCTCCATCTTTAACCTGGGACCAGAATTCATCCCCTTGCAGGAATTGGACCGGAGTTGTTTGCAGAAAACATGGTCAGAGTTGGAGAGTTGTTGGTCTTGATCTTTCTGGCTTGGGACTTACAGGGTCCATAAGCCCTCACATTGGTAACTTATCCTTCCTCCAATCCCTTCAACTTCAAAACAACAAGTTAACAGGAGCAATTCCTACTCAAGTTGTTAACCTTTTCCGCATGAGATCTCTGAACCTGAGCTCCAACGCCATACAAGGTCCACTACCTTCAAACATGAGCCACTTGACTGCACTAGAAATGCTTGACTTGGCAGGGAATAACATAACAGGGAGGCTACCTGAAGAGATATTCAGCCAAAGAAAACTTCGAGTCTTGAATCTGGGACGAAACAGTTTCTTTGGTTCAATTCCTTCATCCATAAGCAACCTCTCATCCACGCTCACATATTTGAATTTGGGTACAAATAGCCTCAGTGGGATCATACCTAGTGAGTTGGGTCTACTTATCAATCTCAAGGAACTTGATCTTTCTGGTAACAAGTTCACTGGGACTGTTGCTCCCTCCATATACAACATTACTTCATTGGTTCTGTTTACTGTTGCTTCAAATCAACTTTGGGGTGAGATTCCCAAAAATATTGATGAAACACTTCCAAATCTTTTACACTACCGGAACTGCTTTAATCTTATGACGGGGAAAATTCCAGCGTCTTTGCACAATATCACCAAGATTCGATCAATTCGAATGTCAAACAATTTTCTGGAAGGAACAGTTCCACCAGGGCTGGGAAACATGCCATTCCTAGAAATGTACAACATTGGCTTCAATAGGATCATTAGTAAAGGCAGTGATGGTCTCAGTTTTATCACTTCCTTGACAAACAGCACCAACCTGCAATATCTTGCTATTGACGACAATCAGTTAGAGGGAGTCATTCCAGAATCACTTGGCAATCTCTCGAAGGTTCTCTCAAAGTTGTACATGGGAGGTAATCGGATTTATGGTAACATTCCTGCCTCAGTTGGTCATCTTAGTCGTTTAGCTTTGTTAAATGTGAGCTACAATTCAATATCCGGTGAAATTCCCACTGAAATAGGCCAATTGGAAAATCTACAAATGCTTGGTTTAGCAGGAAATCAACTGTCTGGTCATATTCCAAACTCTTTAGGAAATCTCAAAAAATTAAACGATCTTGATTTATCTGGGAATCACTTTGTTGGCCAAATACCCTCTTCTTTTTCAAACTTCCAGAATCTGCTTTCCATGGACTTATCAAACAATCAGCTCAACGGAACCATTTCCGGTGAAACTCTCAAACTTCCTAGTTTGAGCACTATTCTCAATCTGTCCAAAAACTTTCTAAGTGGACCTTTGCCTTCAGAAATTGGGTCCCTTGAAAGTGTTGTCACTATAGACCTATCTGACAATGCATTATCTGGAGATATCCCTGGATCAATAGAAAAGTGTAAAAGCTTGGAGAGATTGTTGTTGTCAAGAAATAGAATTTCAGGTCCTATTCCCAATGGTCTAGGAGAATTGAGAGGCCTTGAGTTTCTAGACCTCTCCTCAAACCAGCTGTCCAGCTCCATTCCAGAGAATTTTCAAGACCTACATGCCCTTCGATACTTGAACCTATCATTTAATAACTTAGAAGGAGTGATTCCTAACGGTGGACTATTTGTTACAAATTTCACTAATGTTCATCTTGAAGGCAACCCAAAAATTTGCCTCAAATTTCCATGTGTGAAGAATATTGATGGTCGAAGAAGAAAAATACTAGTCCCAGTTATGATCATTACAACAGTCTTCGCAACCATATGCGTTATAGTGGGCTGTTTGGTCTATGCTAAGAAAAGGAAAGGCTGCAGAGCAAAAATTACAGAAACTTGTGATGACTTAGAAGTCAAGGGGCAGCATCAAATGGTATCGTATGAAGAACTCCGTCGCTCAACAGGGAATTTCAACCCGGATAATTTGGTTGGAAGGGGGAGCTTTGGTTCTGTTTATAAAGGGTTTCTTAGAGACCAAGGAATTGAAGTTGCAGTGAAGGTGCTCGATATAAGAACCACAGGGTCTTGGAAGAGTTTCTTGGCAGAGTGTAATGCATTGAGGAGTGTGAGGCACCGAAATCTTGTCAAACTGATCACCTCCTGCTCAAGCTTAGACTCTAAGAACATGGAGTTTCTGGCTTTGGTTTATGAGTATCTGAGTAATGGGAGCTTGGAAGATTGGATTAAGGGCAAGAGAACAAATTCAGATGGAGACGGTTTGAGCATTGTGGAGAGATTGAATGCGGCAATTGATGTAGCTTGTGGATTGGATTACCTCCATAATGATTGTGAAGTTCCAGTGGCTCACTGTGATTTGAAGCCAAGCAACATTCTTTTGGACAAAGACATGACTGCAAGGGTTGGAGATTTTGGGCTGGCAAGGTTACTGATACAAGAAACATCCACTCAACATTCTATTGGCTCCACCAATGTCCTCAAGGGTTCCATAGGGTATATACCCCCAGAATATGGTTTTGGACATAAACCCTCCACAGCTGGAGATGCATACAGTTTTGGAGTAATGTTGTTGGAGCTGTTTACTGGAAAAAGTCCAACCGATGAAAGCTTCACAGGAGAAGTTAACCTCGTTCAATGGGTGCAGTCATCTTTTCCCCATAACATTGCACAAGTACTAGACTCTGAGTTGTTACACTATGAGCACGACGAAGACTCAAATCCCATCTCAGAAGAAGATCTGAATTGTCTGATCTCAGTTATCGAGGTTGGGCTCTCTTGCACCTTCGCCTCTCCTGATGGACGGATTAGCTTGAGGGATGCCCTTCACAAACTTGAAACTGCTAGAGAGACCTTTTTGAAGCTTGCTCACCTTGACAGTGCTGAGTGTAAATTTCGACAACAAATGCAGGTTGAGGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009987 GO:0010204 GO:0010359 GO:0012501 GO:0012505 GO:0016020 GO:0016045 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031347 GO:0031349 GO:0032409 GO:0032412 GO:0032879 GO:0033554 GO:0034050 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080134 GO:0098543 GO:0098581 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1031

Amino Acids

112.71

Weight (kDa)

5.95

Isoelectric Point (pI)

37.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	37 - 73	4.3e-08	Leucine rich repeat N-terminal domain
LRR_14	PF23598	97 - 232	1.2e-11	Leucine-rich repeat region
LRR_8	PF13855	106 - 164	1.6e-08	Leucine rich repeat
LRR_8	PF13855	176 - 237	2.6e-06	Leucine rich repeat
LRR_14	PF23598	345 - 484	1.3e-11	Leucine-rich repeat region
LRR_8	PF13855	379 - 437	4.4e-06	Leucine rich repeat
LRR_8	PF13855	402 - 461	7.5e-06	Leucine rich repeat
LRR_14	PF23598	467 - 606	4.9e-11	Leucine-rich repeat region
LRR_8	PF13855	501 - 558	6.7e-06	Leucine rich repeat
LRR_8	PF13855	569 - 606	4.1e-07	Leucine rich repeat
Pkinase	PF00069	713 - 930	7.6e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	714 - 928	3.9e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g36850 FvH4_3g36850 FvH4_3g36850
malus_domestica	MD08G1090900.v1.1 MD15G1074900.v1.1 MD15G1075000.v1.1
prunus_persica	Prupe.1G427400_v2.0.a1 Prupe.1G427700_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1
pyrus_communis	pycom15g06980 pycom15g06990
rosa_chinensis	RchiOBHm_Chr5g0066001 RchiOBHm_Chr5g0066021
rosa_laevigata	RLG00000035833 RLG00000035834
rosa_multiflora	Rmu_sc0001473.1_g000006 Rmu_sc0011576.1_g000012 Rmu_sc0011576.1_g000015
rosa_roxburghii	Rroxscaffold_1G00014980 Rroxscaffold_1G00015120
rosa_rugosa	Rorug05G0375800 Rorug05G0375900 Rorug05G0376000
rosa_samantha	Rh5AG434300 Rh5AG434500 Rh5CG472900
rosa_wichuraiana	Rw5G040690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2175
AasI	GACNNNNNNGTC	1 cut(s)	2069
Acc36I	ACCTGC	2 cut(s)	1068, 3073
AccB1I	GGYRCC	1 cut(s)	2289
AccB7I	CCANNNNNTGG	1 cut(s)	2687
AccI	GTMKAC	1 cut(s)	667
AciI	CCGC	2 cut(s)	385, 2462
AclWI	GGATC	3 cut(s)	653, 1007, 1627
AcoI	YGGCCR	1 cut(s)	1387
AcuI	CTGAAG	2 cut(s)	528, 1530
AfaI	GTAC	4 cut(s)	628, 980, 1148, 2850
AfiI	CCNNNNNNNGG	5 cut(s)	195, 299, 953, 1838, 2687
AhlI	ACTAGT	1 cut(s)	1945
AjnI	CCWGG	3 cut(s)	170, 946, 1615
AjuI	GAANNNNNNNTTGG	6 cut(s)	516, 548, 2240, 2272, 2676, 2708
AloI	GAACNNNNNNTCC	4 cut(s)	392, 424, 2149, 2181
Alw21I	GWGCWC	4 cut(s)	410, 1502, 2223, 2880
Alw26I	GTCTC	4 cut(s)	1028, 2184, 2424, 3017
AlwI	GGATC	3 cut(s)	653, 1007, 1627
AlwNI	CAGNNNCTG	2 cut(s)	86, 1423
AoxI	GGCC	5 cut(s)	120, 141, 1264, 1387, 1711
ApeKI	GCWGC	2 cut(s)	2031, 2080
ArsI	GACNNNNNNTTYG	2 cut(s)	1730, 1762
Asp700I	GAANNNNTTC	4 cut(s)	816, 1465, 2252, 2419
AspLEI	GCGC	1 cut(s)	80
AspS9I	GGNCC	7 cut(s)	175, 204, 282, 425, 1535, 1557, 1679
AsuC2I	CCSGG	1 cut(s)	2135
AsuHPI	GGTGA	6 cut(s)	800, 883, 1256, 1484, 2305, 3035
AsuII	TTCGAA	1 cut(s)	910
AvaII	GGWCC	7 cut(s)	175, 204, 282, 425, 1535, 1557, 1679
BalI	TGGCCA	1 cut(s)	1389
BanI	GGYRCC	1 cut(s)	2289
BanII	GRGCYC	2 cut(s)	410, 2949
BbsI	GAAGAC	2 cut(s)	1966, 2892
Bbv12I	GWGCWC	4 cut(s)	410, 1502, 2223, 2880
BbvI	GCAGC	2 cut(s)	2018, 2092
BccI	CCATC	6 cut(s)	169, 1013, 1919, 2420, 2906, 2966
BciT130I	CCWGG	3 cut(s)	172, 948, 1617
BclI	TGATCA	2 cut(s)	1959, 2310
BcnI	CCSGG	1 cut(s)	2135
BcoDI	GTCTC	4 cut(s)	1028, 2184, 2424, 3017
BcuI	ACTAGT	1 cut(s)	1945
BfaI	CTAG	9 cut(s)	461, 654, 971, 1491, 1697, 1724, 1946, 2852, 3018
BfmI	CTRYAG	2 cut(s)	1578, 2032
BfuAI	ACCTGC	2 cut(s)	1068, 3073
BglII	AGATCT	2 cut(s)	392, 2911
BisI	GCNGC	3 cut(s)	2032, 2081, 2463
BlsI	GCNGC	3 cut(s)	2033, 2082, 2464
BmcAI	AGTACT	1 cut(s)	2850
Bme1390I	CCNGG	4 cut(s)	172, 948, 1617, 2135
Bme18I	GGWCC	7 cut(s)	175, 204, 282, 425, 1535, 1557, 1679
BmgT120I	GGNCC	7 cut(s)	175, 204, 282, 425, 1535, 1557, 1679
BmiI	GGNNCC	8 cut(s)	176, 283, 1462, 1558, 1559, 2291, 2641, 2662
BmrFI	CCNGG	4 cut(s)	172, 948, 1617, 2135
BmrI	ACTGGG	2 cut(s)	723, 1946
BmsI	GCATC	3 cut(s)	2092, 2710, 2983
BmuI	ACTGGG	2 cut(s)	723, 1946
BpiI	GAAGAC	2 cut(s)	1966, 2892
BpmI	CTGGAG	2 cut(s)	1626, 2736
Bpu10I	CCTNAGC	1 cut(s)	404
Bpu14I	TTCGAA	1 cut(s)	910
BpuEI	CTTGAG	6 cut(s)	351, 665, 1736, 2308, 2639, 3007
BpuMI	CCSGG	1 cut(s)	2135
BsaI	GGTCTC	3 cut(s)	1028, 2184, 3017
BsaJI	CCNNGG	5 cut(s)	171, 947, 1431, 1615, 2194
BsaWI	WCCGGW	4 cut(s)	206, 837, 1241, 1469
BsaXI	ACNNNNNCTCC	8 cut(s)	392, 422, 709, 739, 1637, 1667, 2149, 2179
Bsc4I	CCNNNNNNNGG	5 cut(s)	195, 299, 953, 1838, 2687
Bse1I	ACTGG	4 cut(s)	718, 1952, 2516, 2764
Bse3DI	GCAATG	1 cut(s)	2838
BseBI	CCWGG	3 cut(s)	172, 948, 1617
BseDI	CCNNGG	5 cut(s)	171, 947, 1431, 1615, 2194
BseGI	GGATG	5 cut(s)	185, 581, 599, 2618, 2998
BseLI	CCNNNNNNNGG	5 cut(s)	195, 299, 953, 1838, 2687
BseMI	GCAATG	1 cut(s)	2838
BseNI	ACTGG	4 cut(s)	718, 1952, 2516, 2764
BseRI	GAGGAG	3 cut(s)	21, 1723, 2293
BseXI	GCAGC	2 cut(s)	2018, 2092
BseYI	CCCAGC	1 cut(s)	952
BsgI	GTGCAG	2 cut(s)	441, 2837
BshFI	GGCC	5 cut(s)	122, 143, 1266, 1389, 1713
BshNI	GGYRCC	1 cut(s)	2289
BsiHKAI	GWGCWC	4 cut(s)	410, 1502, 2223, 2880
BsiSI	CCGG	5 cut(s)	207, 838, 1242, 1470, 2135
BslFI	GGGAC	7 cut(s)	140, 188, 285, 564, 730, 1543, 1934
BslI	CCNNNNNNNGG	5 cut(s)	195, 299, 953, 1838, 2687
BsmAI	GTCTC	4 cut(s)	1028, 2184, 2424, 3017
BsmBI	CGTCTC	1 cut(s)	2424
BsmFI	GGGAC	7 cut(s)	140, 188, 285, 564, 730, 1543, 1934
BsmI	GAATGC	1 cut(s)	2464
BsnI	GGCC	5 cut(s)	122, 143, 1266, 1389, 1713
Bso31I	GGTCTC	3 cut(s)	1028, 2184, 3017
Bsp119I	TTCGAA	1 cut(s)	910
Bsp1286I	GDGCHC	5 cut(s)	410, 1502, 2223, 2880, 2949
Bsp1407I	TGTACA	2 cut(s)	978, 1146
Bsp19I	CCATGG	1 cut(s)	1431
BspACI	CCGC	2 cut(s)	385, 2462
BspANI	GGCC	5 cut(s)	122, 143, 1266, 1389, 1713
BspLI	GGNNCC	8 cut(s)	176, 283, 1462, 1558, 1559, 2291, 2641, 2662
BspMAI	CTGCAG	1 cut(s)	2036
BspMI	ACCTGC	2 cut(s)	1068, 3073
BspPI	GGATC	3 cut(s)	653, 1007, 1627
BspQI	GCTCTTC	1 cut(s)	57
BspT104I	TTCGAA	1 cut(s)	910
BspT107I	GGYRCC	1 cut(s)	2289
BspTNI	GGTCTC	3 cut(s)	1028, 2184, 3017
BsrDI	GCAATG	1 cut(s)	2838
BsrGI	TGTACA	2 cut(s)	978, 1146
BsrI	ACTGG	4 cut(s)	718, 1952, 2516, 2764
BssECI	CCNNGG	5 cut(s)	171, 947, 1431, 1615, 2194
BssT1I	CCWWGG	2 cut(s)	1431, 2194
Bst2UI	CCWGG	3 cut(s)	172, 948, 1617
Bst6I	CTCTTC	4 cut(s)	57, 504, 1405, 2243
BstAPI	GCANNNNNTGC	1 cut(s)	86
BstAUI	TGTACA	2 cut(s)	978, 1146
BstBI	TTCGAA	1 cut(s)	910
BstC8I	GCNNGC	2 cut(s)	2595, 3039
BstDSI	CCRYGG	1 cut(s)	1431
BstF5I	GGATG	5 cut(s)	185, 581, 599, 2618, 2998
BstHHI	GCGC	1 cut(s)	80
BstMAI	GTCTC	4 cut(s)	1028, 2184, 2424, 3017
BstMWI	GCNNNNNNNGC	2 cut(s)	86, 2720
BstNI	CCWGG	3 cut(s)	172, 948, 1617
BstNSI	RCATGY	2 cut(s)	964, 1784
BstSCI	CCNGG	4 cut(s)	170, 946, 1615, 2133
BstSFI	CTRYAG	2 cut(s)	1578, 2032
BstV1I	GCAGC	2 cut(s)	2018, 2092
BstV2I	GAAGAC	2 cut(s)	1966, 2892
BstX2I	RGATCY	2 cut(s)	392, 2911
BstYI	RGATCY	2 cut(s)	392, 2911
BsuRI	GGCC	5 cut(s)	122, 143, 1266, 1389, 1713
BtgI	CCRYGG	1 cut(s)	1431
BtsCI	GGATG	5 cut(s)	185, 581, 599, 2618, 2998
BtsI	GCAGTG	2 cut(s)	1021, 2217
BtsIMutI	CAGTG	8 cut(s)	646, 711, 1021, 1254, 2217, 2523, 2524, 3058
BveI	ACCTGC	2 cut(s)	1068, 3073
Cac8I	GCNNGC	2 cut(s)	2595, 3039
CaiI	CAGNNNCTG	2 cut(s)	86, 1423
CfoI	GCGC	1 cut(s)	80
Cfr13I	GGNCC	7 cut(s)	175, 204, 282, 425, 1535, 1557, 1679
CseI	GACGC	1 cut(s)	864
Csp6I	GTAC	4 cut(s)	627, 979, 1147, 2849
CviQI	GTAC	4 cut(s)	627, 979, 1147, 2849
DrdI	GACNNNNNNGTC	1 cut(s)	2069
DseDI	GACNNNNNNGTC	1 cut(s)	2069
EaeI	YGGCCR	1 cut(s)	1387
Eam1104I	CTCTTC	4 cut(s)	57, 504, 1405, 2243
EarI	CTCTTC	4 cut(s)	57, 504, 1405, 2243
Ecl136II	GAGCTC	1 cut(s)	408
Eco130I	CCWWGG	2 cut(s)	1431, 2194
Eco147I	AGGCCT	3 cut(s)	122, 143, 1713
Eco24I	GRGCYC	2 cut(s)	410, 2949
Eco31I	GGTCTC	3 cut(s)	1028, 2184, 3017
Eco32I	GATATC	1 cut(s)	1612
Eco47I	GGWCC	7 cut(s)	175, 204, 282, 425, 1535, 1557, 1679
Eco53kI	GAGCTC	1 cut(s)	408
Eco57I	CTGAAG	2 cut(s)	528, 1530
EcoICRI	GAGCTC	1 cut(s)	408
EcoO109I	RGGNCCY	2 cut(s)	1557, 1679
EcoRI	GAATTC	1 cut(s)	181
EcoRII	CCWGG	3 cut(s)	170, 946, 1615
EcoRV	GATATC	1 cut(s)	1612
EcoT14I	CCWWGG	2 cut(s)	1431, 2194
EcoT22I	ATGCAT	4 cut(s)	65, 1600, 2275, 2725
EcoT38I	GRGCYC	2 cut(s)	410, 2949
ErhI	CCWWGG	2 cut(s)	1431, 2194
Esp3I	CGTCTC	1 cut(s)	2424
FaqI	GGGAC	7 cut(s)	140, 188, 285, 564, 730, 1543, 1934
FauNDI	CATATG	1 cut(s)	1985
FbaI	TGATCA	2 cut(s)	1959, 2310
FblI	GTMKAC	1 cut(s)	667
Fnu4HI	GCNGC	3 cut(s)	2032, 2081, 2463
FokI	GGATG	5 cut(s)	172, 568, 586, 2605, 3005
FriOI	GRGCYC	2 cut(s)	410, 2949
Fsp4HI	GCNGC	3 cut(s)	2032, 2081, 2463
FspBI	CTAG	9 cut(s)	461, 654, 971, 1491, 1697, 1724, 1946, 2852, 3018
GlaI	GCGC	1 cut(s)	79
GluI	GCNGC	3 cut(s)	2032, 2081, 2463
GsaI	CCCAGC	1 cut(s)	956
GsuI	CTGGAG	2 cut(s)	1626, 2736
HaeIII	GGCC	5 cut(s)	122, 143, 1266, 1389, 1713
HapII	CCGG	5 cut(s)	207, 838, 1242, 1470, 2135
HgaI	GACGC	1 cut(s)	864
HhaI	GCGC	1 cut(s)	80
Hin6I	GCGC	1 cut(s)	78
HinP1I	GCGC	1 cut(s)	78
HincII	GTYRAC	3 cut(s)	348, 376, 2800
HindII	GTYRAC	3 cut(s)	348, 376, 2800
HindIII	AAGCTT	4 cut(s)	1639, 2326, 2782, 3035
HpaI	GTTAAC	3 cut(s)	348, 376, 2800
HpaII	CCGG	5 cut(s)	207, 838, 1242, 1470, 2135
HphI	GGTGA	6 cut(s)	800, 883, 1256, 1484, 2305, 3035
Hpy99I	CGWCG	2 cut(s)	2115, 2885
HpyF10VI	GCNNNNNNNGC	2 cut(s)	86, 2720
HspAI	GCGC	1 cut(s)	78
KflI	GGGWCCC	1 cut(s)	1557
Ksp22I	TGATCA	2 cut(s)	1959, 2310
KspAI	GTTAAC	3 cut(s)	348, 376, 2800
LguI	GCTCTTC	1 cut(s)	57
LmnI	GCTCC	8 cut(s)	353, 413, 730, 1757, 2157, 2385, 2645, 2749
Lsp1109I	GCAGC	2 cut(s)	2018, 2092
LweI	GCATC	3 cut(s)	2092, 2710, 2983
MaeI	CTAG	9 cut(s)	461, 654, 971, 1491, 1697, 1724, 1946, 2852, 3018
MaeIII	GTNAC	7 cut(s)	302, 701, 1172, 1573, 1849, 2601, 2865
MfeI	CAATTG	2 cut(s)	1268, 2466
MflI	RGATCY	2 cut(s)	392, 2911
MhlI	GDGCHC	5 cut(s)	410, 1502, 2223, 2880, 2949
MlsI	TGGCCA	1 cut(s)	1389
MluNI	TGGCCA	1 cut(s)	1389
MlyI	GAGTC	6 cut(s)	92, 546, 1107, 2327, 2849, 2882
MmeI	TCCRAC	6 cut(s)	218, 435, 2128, 2560, 2727, 2795
Mox20I	TGGCCA	1 cut(s)	1389
Mph1103I	ATGCAT	4 cut(s)	65, 1600, 2275, 2725
MroXI	GAANNNNTTC	4 cut(s)	816, 1465, 2252, 2419
MscI	TGGCCA	1 cut(s)	1389
MseI	TTAA	9 cut(s)	167, 347, 375, 849, 1217, 1352, 1809, 2403, 2799
Msp20I	TGGCCA	1 cut(s)	1389
MspA1I	CMGCKG	2 cut(s)	1744, 2714
MspI	CCGG	5 cut(s)	207, 838, 1242, 1470, 2135
MspR9I	CCNGG	4 cut(s)	172, 948, 1617, 2135
MunI	CAATTG	2 cut(s)	1268, 2466
Mva1269I	GAATGC	1 cut(s)	2464
MvaI	CCWGG	3 cut(s)	172, 948, 1617
MwoI	GCNNNNNNNGC	2 cut(s)	86, 2720
NciI	CCSGG	1 cut(s)	2135
NcoI	CCATGG	1 cut(s)	1431
NdeI	CATATG	1 cut(s)	1985
NlaIV	GGNNCC	8 cut(s)	176, 283, 1462, 1558, 1559, 2291, 2641, 2662
NmuCI	GTSAC	1 cut(s)	1573
NsiI	ATGCAT	4 cut(s)	65, 1600, 2275, 2725
NspI	RCATGY	2 cut(s)	964, 1784
NspV	TTCGAA	1 cut(s)	910
PceI	AGGCCT	3 cut(s)	122, 143, 1713
PciSI	GCTCTTC	1 cut(s)	57
PcsI	WCGNNNNNNNCGW	1 cut(s)	907
PctI	GAATGC	1 cut(s)	2464
PdmI	GAANNNNTTC	4 cut(s)	816, 1465, 2252, 2419
PfeI	GAWTC	8 cut(s)	5, 544, 793, 898, 1109, 1375, 1420, 1828
PflMI	CCANNNNNTGG	1 cut(s)	2687
PkrI	GCNGC	3 cut(s)	2033, 2082, 2464
PleI	GAGTC	6 cut(s)	91, 545, 1106, 2327, 2849, 2882
PpsI	GAGTC	6 cut(s)	91, 545, 1106, 2327, 2849, 2882
PpuMI	RGGWCCY	2 cut(s)	1557, 1679
PsiI	TTATAA	1 cut(s)	2175
Psp124BI	GAGCTC	1 cut(s)	410
Psp5II	RGGWCCY	2 cut(s)	1557, 1679
Psp6I	CCWGG	3 cut(s)	170, 946, 1615
PspFI	CCCAGC	1 cut(s)	952
PspGI	CCWGG	3 cut(s)	170, 946, 1615
PspN4I	GGNNCC	8 cut(s)	176, 283, 1462, 1558, 1559, 2291, 2641, 2662
PspPI	GGNCC	7 cut(s)	175, 204, 282, 425, 1535, 1557, 1679
PspPPI	RGGWCCY	2 cut(s)	1557, 1679
PstI	CTGCAG	1 cut(s)	2036
PstNI	CAGNNNCTG	2 cut(s)	86, 1423
PsuI	RGATCY	2 cut(s)	392, 2911
PvuII	CAGCTG	2 cut(s)	1744, 2714
RsaI	GTAC	4 cut(s)	628, 980, 1148, 2850
RsaNI	GTAC	4 cut(s)	627, 979, 1147, 2849
SacI	GAGCTC	1 cut(s)	410
SapI	GCTCTTC	1 cut(s)	57
SaqAI	TTAA	9 cut(s)	167, 347, 375, 849, 1217, 1352, 1809, 2403, 2799
SatI	GCNGC	3 cut(s)	2032, 2081, 2463
Sau96I	GGNCC	7 cut(s)	175, 204, 282, 425, 1535, 1557, 1679
ScaI	AGTACT	1 cut(s)	2850
SchI	GAGTC	6 cut(s)	92, 546, 1107, 2327, 2849, 2882
ScrFI	CCNGG	4 cut(s)	172, 948, 1617, 2135
SduI	GDGCHC	5 cut(s)	410, 1502, 2223, 2880, 2949
SfaNI	GCATC	3 cut(s)	2092, 2710, 2983
SfcI	CTRYAG	2 cut(s)	1578, 2032
SfuI	TTCGAA	1 cut(s)	910
SinI	GGWCC	7 cut(s)	175, 204, 282, 425, 1535, 1557, 1679
SmlI	CTYRAG	6 cut(s)	366, 680, 1715, 2323, 2654, 2986
SmoI	CTYRAG	6 cut(s)	366, 680, 1715, 2323, 2654, 2986
SpeI	ACTAGT	1 cut(s)	1945
SseBI	AGGCCT	3 cut(s)	122, 143, 1713
SsiI	CCGC	2 cut(s)	385, 2462
SspI	AATATT	2 cut(s)	805, 1921
SspMI	CTAG	9 cut(s)	461, 654, 971, 1491, 1697, 1724, 1946, 2852, 3018
SstI	GAGCTC	1 cut(s)	410
StuI	AGGCCT	3 cut(s)	122, 143, 1713
StyD4I	CCNGG	4 cut(s)	170, 946, 1615, 2133
StyI	CCWWGG	2 cut(s)	1431, 2194
TatI	WGTACW	3 cut(s)	978, 1146, 2848
TauI	GCSGC	1 cut(s)	2465
TfiI	GAWTC	8 cut(s)	5, 544, 793, 898, 1109, 1375, 1420, 1828
Tru1I	TTAA	9 cut(s)	167, 347, 375, 849, 1217, 1352, 1809, 2403, 2799
Tru9I	TTAA	9 cut(s)	167, 347, 375, 849, 1217, 1352, 1809, 2403, 2799
TscAI	CASTG	8 cut(s)	646, 718, 1021, 1261, 2217, 2523, 2531, 3058
TseFI	GTSAC	1 cut(s)	1573
TseI	GCWGC	2 cut(s)	2031, 2080
Tsp45I	GTSAC	1 cut(s)	1573
TspDTI	ATGAA	7 cut(s)	174, 570, 738, 825, 1859, 2115, 2793
TspGWI	ACGGA	3 cut(s)	1473, 2099, 2992
TspRI	CASTG	8 cut(s)	646, 718, 1021, 1261, 2217, 2523, 2531, 3058
Van91I	CCANNNNNTGG	1 cut(s)	2687
VpaK11BI	GGWCC	7 cut(s)	175, 204, 282, 425, 1535, 1557, 1679
XbaI	TCTAGA	1 cut(s)	1723
XceI	RCATGY	2 cut(s)	964, 1784
XcmI	CCANNNNNNNNNTGG	1 cut(s)	168
XmiI	GTMKAC	1 cut(s)	667
XmnI	GAANNNNTTC	4 cut(s)	816, 1465, 2252, 2419
XspI	CTAG	9 cut(s)	461, 654, 971, 1491, 1697, 1724, 1946, 2852, 3018
ZrmI	AGTACT	1 cut(s)	2850
Zsp2I	ATGCAT	4 cut(s)	65, 1600, 2275, 2725

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.