Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005533

Physical Location & Seq

Genomic Coordinates Reverse (-)

18800314 .. 18821645

Gene Structure 21332 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00015120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3387 bp

ATGGATTCCCACTTCCATACTCTACTGCCTCCTCAGATTTCCATGTTAGCTCTTCTTCTATGCATATCATTTCTGAGCGCAGAGTCTGCTATAATTCCAAGCATTACCACCGACAAGGAGGCCTTGATCTCATTCAAGGAGGCCTTGCTCGATCTCCCTCCATCTTTAAGCTGGGACCAGAATTCATCCCCTTGCAGCAATTGGACCGGAGTTGTTTGCAGAAAACATGGTCAGAGTCAGAGAGTTGTTGTTCTTGATCTTTCAGGCTTGGGACTTACAGAAACTAATATTATTTCACAAGTTATTGTCAAAATACAGTTAGACCTGGTGAAGCTATTGCTACTAGGGCTATCAATGGGTCATGTCGGGTTGGGGTCCATAAGCCCTCACATTGGTAACTTATCCTTCCTCCAATCTCTTCAACTTCAAAACAATAAGTTAACAGGAGCAATTCCTACACAAGTTGTTAAACTTTTCCGCTTGAGATCTCTGAACCTAAGCTCCAACACCATACAAGGTCCCCTACCTTCAAACATGAGCCACTTGACTGCACTAGAAACGCTTGACTTGGCGGGGAATAACATAACAGGGTGGCTCCCTGAGGAGATATTCAGCCAAAAAGAACTTCAAGTCCTGAATCTGGGACGAAACAATTTCCTTGGTTCACTTCCTTCATCCATAAGCAACCTCTCATCCACGCTCACATATTTGAATTTGGGTACAAATAGCCTCAGTGGGATCATACCTAGTGAGTTGGGTCTACTTATCAATCTCAAGGAACTAGATCTTTCTGGTAACAAGTTCACTGGGACTGTTGCTCCCTCCATATACAACATTACTTCATTGGTTCTGTTTACTGTTGCTTCAAATCAACTTTGGGGTGAGATTCCCAAAAATATTGATGAAACACTTCCAAATCTTTTATACTACCGGAACTGCTTTAATCTTATGACCGGGAAAATTCCAGCGTCTTTGCACAATATCACCAAGATTCGATCAATTCGAATGTCAAACAATTTTCTGGAAGGAACAGTTCCACCAGGGCTGGGAAACATGCCATTCCTTGAAATGTACAACATTGGCTTCAATCGGATCGTCAGTAAAGGCAGTGATGGTCTCAGTTTTATCACTTCCTTGACGAACAGCACCAACCTGCAATTTCTTGCTATCGACCACAATCAGTTAGAGGGAGTCATTCCAGAATCACTTGGCAATCTCTCAAAGGTTCTCTCAAAATTGTACATGGGAGGGAATCGGATTTATGGTAACATTCCTGCCTCAGTTGGTCATCTTAGTGGTTTAACTTTGTTAAATGTGAGCTACAATTCAATATCTGGTGAAATTCCCACTGAAATAGGCCAATTGGAAAACCTACAAATGCTTGGTTTAGCAGGAAATGAAATGTCTGGTCATATTCCAAACTCTTTAGGAAATCTCAAAAAGCTGAACGGTATTGATTTATCTGGGAATCACTTTGTCGGCCAAATACCCTCTTCTTTTTCAAACTTCCAGAGTTTGCTTTCCATGGACTTGTCAAACAATCAGCTCAACGGAACCATTTCCGGTGAAACTCTCAACCTTCCTAGTTTGAGCACCATTCTCAATCTGTCCAAAAACTTTCTAAGTGGACCTTTGCCTTCAGAAATTGGGTCCCTTAAAAGTGTTGTCACTATAGACCTCTCTGACAATGCATTATCTGGAGATATCCCTGGATCAATAGGAAAGTGTAAAAGCTTGGAGAGATTGTTGATGTCAAGAAATAGAATTTCAGGTCCTATTCCCACTGGTCTAGGAGAATTAAGAGGCCTTGACTTTCTAGACCTCTCCTCAAACCAGCTGTCCAGCTCCATTCCAGAGAATTTTCGAGACCTACATGCCCTTCGATACTTGAACCTATCATTTAATAACTTAGAAGGAGTGATTCCTAATGGTGGAGTATTTGTTACAAATTTCAGTAATGTTCATCTTGAAGGCAACCCAAAAATTTGCCTCAAATTTCCATGTGAGAAGAATACTGATGTTCATCGAAGAAGAAAAATACTAGTCCTAGTTATGATCATTACAACAGTCTTAGCAACCATATGTGTTATAGTGGGCTCTTTGGTCTATGCTAAGACAAGAAAAGGCTGCAAAGCAAAAATTACAGAAACTTGTGATAACGTAGTAGTAAAAGGGCAGCATCAGATGGTATCGTATGAAGAACTTCGTCGCTCAACAGGGAATTTCAACCCGGATAATTTGGTTGGAAGGGGGAGCTTTGGTTCTGTTTATAAAGGGTTTCTTAGAGACCAAGGAATTGAAGTTGCAGTCAAGGTGCTCGATATAAGAACAACAGGGTCTTGGAAGAGTTTCTTGGCAGAGTGTAATGCATTAAGGAGTGTGAGGCACCGAAATCTTGTCAAACTGATCACCTCCTGCTCAAGCTTAGACTGTAAGAACATGGAGTTTCTGGCTTTGGTTTATGAGTATCTGAGTAATGGGAGCTTGGAAGATTGGATTAAGGGCAAGAGAACAAATTCAGATGGAAACGGTTTGAACATTGTGGAGAGATTGAATGTGGCAATTGATGTTGCTTGTGGATTGGATTACCTCCATAATGATTGTGAAGTTCCAGTGGCTCACTGTGATTTGAAGCCAAGCAACATTCTTTTGGAGAAAGACATGACTGCAAAGGTTGGAGATTTTGGGCTGGCAAAGTTACTGATACAGGAAGCATCCACTCAACATTCTATTGGCTCCACCAATGTCCTCAAGGGTTCCATAGGATATATACCCCCAGAATATGGTTTTGGAAAAAAACCCTCGACAGCTGGAGATGCATACAGTTTTGGAGTAATGTTGTTGGAGCTGTTTACTGGAAAAAGTCCAACCGGTGAAAGCTTCATAGGAGAAGTTAACCTCGTTCAGTGGGTGCAGTCATCTTTTCCCCACAACATTGCACAAGTACTAGACTCTGAGTTGTTACATTATGAGAACGAAGAAGGCTCAAATCCCATCTCAGAAGAAGAACTGAATTGTTTGATCTCAGTTATCGAGGTTGGGCTCTCTTGCACCTTCACCTCTCCTGATGGACGGATTAGCTTGAGGGATGCCCTTCACAAACTTGAAACTGCTAGAAAGACCCTTTTGAAGCTTGCTCAGTTTGACAGTGCAGACTGCAGAGTATGGCTGCTGCGAAGGTTGAGCATGGAGACCTGTGGAAGAGCAATACTCTCTCGGCACAACACCGGCTCGGGAAGCGGTTCGAGTCGAAGTGGACCGCCGGTGCGTACACCGTCCGGTTTCGGCGACAGCTTGCTTCGCCTCCACCGTGCGTTGTTGGCTTCAGCAGTTCGTGATTTAAAGGAGGGCTCGCTTCTGTCCTCCACCGTCTTCTATCGTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009987 GO:0010204 GO:0010359 GO:0012501 GO:0012505 GO:0016020 GO:0016045 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031347 GO:0031349 GO:0032409 GO:0032412 GO:0032879 GO:0033554 GO:0034050 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080134 GO:0098543 GO:0098581 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1128

Amino Acids

122.54

Weight (kDa)

7.09

Isoelectric Point (pI)

37.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	37 - 73	6.1e-09	Leucine rich repeat N-terminal domain
LRR_14	PF23598	84 - 190	4.7e-09	Leucine-rich repeat region
LRR_8	PF13855	137 - 195	2.1e-08	Leucine rich repeat
LRR_8	PF13855	208 - 268	7e-06	Leucine rich repeat
LRR_8	PF13855	410 - 467	5e-06	Leucine rich repeat
LRR_14	PF23598	422 - 485	5.4e-06	Leucine-rich repeat region
LRR_14	PF23598	498 - 637	5.4e-11	Leucine-rich repeat region
LRR_8	PF13855	600 - 637	4e-07	Leucine rich repeat
Pkinase	PF00069	745 - 958	1.1e-39	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	746 - 981	1.5e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g36850 FvH4_3g36850 FvH4_3g36850
malus_domestica	MD08G1090900.v1.1 MD15G1074900.v1.1 MD15G1075000.v1.1
prunus_persica	Prupe.1G427400_v2.0.a1 Prupe.1G427700_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1
pyrus_communis	pycom15g06980 pycom15g06990
rosa_chinensis	RchiOBHm_Chr5g0066001 RchiOBHm_Chr5g0066021
rosa_laevigata	RLG00000035833 RLG00000035834
rosa_multiflora	Rmu_sc0001473.1_g000006 Rmu_sc0011576.1_g000012 Rmu_sc0011576.1_g000015
rosa_roxburghii	Rroxscaffold_1G00014980 Rroxscaffold_1G00015120
rosa_rugosa	Rorug05G0375800 Rorug05G0375900 Rorug05G0376000
rosa_samantha	Rh5AG434300 Rh5AG434500 Rh5CG472900
rosa_wichuraiana	Rw5G040690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2271
Acc36I	ACCTGC	1 cut(s)	1161
AccB1I	GGYRCC	1 cut(s)	2385
AccB7I	CCANNNNNTGG	1 cut(s)	2783
AccI	GTMKAC	1 cut(s)	760
AciI	CCGC	4 cut(s)	478, 572, 3240, 3260
AclWI	GGATC	3 cut(s)	746, 1100, 1720
AcoI	YGGCCR	1 cut(s)	1480
AcuI	CTGAAG	2 cut(s)	1623, 3309
AfaI	GTAC	5 cut(s)	721, 1073, 1241, 2946, 3271
AfiI	CCNNNNNNNGG	5 cut(s)	392, 640, 1046, 1931, 2783
AgeI	ACCGGT	1 cut(s)	2870
AhlI	ACTAGT	1 cut(s)	2041
AjnI	CCWGG	3 cut(s)	324, 1039, 1708
AjuI	GAANNNNNNNTTGG	6 cut(s)	609, 641, 2336, 2368, 2772, 2804
AloI	GAACNNNNNNTCC	6 cut(s)	485, 517, 615, 647, 2245, 2277
Alw21I	GWGCWC	2 cut(s)	1595, 2319
Alw26I	GTCTC	4 cut(s)	1121, 1860, 2280, 3185
AlwI	GGATC	3 cut(s)	746, 1100, 1720
AlwNI	CAGNNNCTG	1 cut(s)	86
Ama87I	CYCGRG	1 cut(s)	3232
AoxI	GGCC	5 cut(s)	120, 141, 1357, 1480, 1804
ApeKI	GCWGC	5 cut(s)	195, 2127, 2176, 3169, 3172
ArsI	GACNNNNNNTTYG	2 cut(s)	1823, 1855
AsiGI	ACCGGT	1 cut(s)	2870
Asp700I	GAANNNNTTC	6 cut(s)	909, 1558, 2202, 2348, 2515, 3241
AspLEI	GCGC	1 cut(s)	80
AspS9I	GGNCC	8 cut(s)	175, 204, 375, 518, 1628, 1650, 1772, 3257
AsuC2I	CCSGG	2 cut(s)	955, 2231
AsuHPI	GGTGA	8 cut(s)	340, 893, 976, 1349, 1577, 2401, 2885, 3049
AsuII	TTCGAA	1 cut(s)	1003
AvaI	CYCGRG	1 cut(s)	3232
AvaII	GGWCC	8 cut(s)	175, 204, 375, 518, 1628, 1650, 1772, 3257
AxyI	CCTNAGG	1 cut(s)	600
BanI	GGYRCC	1 cut(s)	2385
BanII	GRGCYC	3 cut(s)	2099, 3045, 3353
BbsI	GAAGAC	1 cut(s)	3364
Bbv12I	GWGCWC	2 cut(s)	1595, 2319
BbvI	GCAGC	5 cut(s)	207, 2114, 2188, 3156, 3159
BccI	CCATC	6 cut(s)	169, 1106, 2179, 2516, 3002, 3062
BciT130I	CCWGG	3 cut(s)	326, 1041, 1710
BclI	TGATCA	2 cut(s)	2055, 2406
BcnI	CCSGG	2 cut(s)	955, 2231
BcoDI	GTCTC	4 cut(s)	1121, 1860, 2280, 3185
BcuI	ACTAGT	1 cut(s)	2041
BfmI	CTRYAG	2 cut(s)	1671, 3157
BfuAI	ACCTGC	1 cut(s)	1161
BglII	AGATCT	2 cut(s)	485, 784
BisI	GCNGC	5 cut(s)	196, 2128, 2177, 3170, 3173
BlsI	GCNGC	5 cut(s)	197, 2129, 2178, 3171, 3174
BmcAI	AGTACT	1 cut(s)	2946
Bme1390I	CCNGG	5 cut(s)	326, 955, 1041, 1710, 2231
Bme18I	GGWCC	8 cut(s)	175, 204, 375, 518, 1628, 1650, 1772, 3257
BmeT110I	CYCGRG	1 cut(s)	3232
BmgT120I	GGNCC	8 cut(s)	175, 204, 375, 518, 1628, 1650, 1772, 3257
BmrFI	CCNGG	5 cut(s)	326, 955, 1041, 1710, 2231
BmrI	ACTGGG	1 cut(s)	816
BmsI	GCATC	4 cut(s)	2188, 2723, 2806, 3079
BmuI	ACTGGG	1 cut(s)	816
BpiI	GAAGAC	1 cut(s)	3364
BplI	GAGNNNNNCTC	2 cut(s)	3195, 3227
BpmI	CTGGAG	2 cut(s)	1719, 2832
Bpu10I	CCTNAGC	1 cut(s)	497
Bpu14I	TTCGAA	1 cut(s)	1003
BpuEI	CTTGAG	5 cut(s)	502, 758, 2404, 2735, 3103
BpuMI	CCSGG	2 cut(s)	955, 2231
BsaBI	GATNNNNATC	1 cut(s)	2022
BsaI	GGTCTC	4 cut(s)	1121, 1860, 2280, 3185
BsaJI	CCNNGG	5 cut(s)	658, 1040, 1524, 1708, 2290
BsaWI	WCCGGW	5 cut(s)	206, 930, 1562, 2870, 3278
Bsc4I	CCNNNNNNNGG	5 cut(s)	392, 640, 1046, 1931, 2783
Bse118I	RCCGGY	3 cut(s)	2870, 3227, 3262
Bse1I	ACTGG	4 cut(s)	811, 1789, 2612, 2860
Bse21I	CCTNAGG	1 cut(s)	600
Bse3DI	GCAATG	1 cut(s)	2934
Bse8I	GATNNNNATC	1 cut(s)	2022
BseBI	CCWGG	3 cut(s)	326, 1041, 1710
BseDI	CCNNGG	5 cut(s)	658, 1040, 1524, 1708, 2290
BseGI	GGATG	5 cut(s)	185, 674, 692, 2714, 3094
BseJI	GATNNNNATC	1 cut(s)	2022
BseLI	CCNNNNNNNGG	5 cut(s)	392, 640, 1046, 1931, 2783
BseMI	GCAATG	1 cut(s)	2934
BseNI	ACTGG	4 cut(s)	811, 1789, 2612, 2860
BseRI	GAGGAG	3 cut(s)	21, 617, 1816
BseXI	GCAGC	5 cut(s)	207, 2114, 2188, 3156, 3159
BseYI	CCCAGC	2 cut(s)	171, 1045
BsgI	GTGCAG	3 cut(s)	534, 2933, 3171
BshFI	GGCC	5 cut(s)	122, 143, 1359, 1482, 1806
BshNI	GGYRCC	1 cut(s)	2385
BshTI	ACCGGT	1 cut(s)	2870
BsiHKAI	GWGCWC	2 cut(s)	1595, 2319
BsiHKCI	CYCGRG	1 cut(s)	3232
BsiSI	CCGG	9 cut(s)	207, 931, 954, 1563, 2231, 2871, 3228, 3263, 3279
BslFI	GGGAC	6 cut(s)	188, 285, 504, 657, 823, 1636
BslI	CCNNNNNNNGG	5 cut(s)	392, 640, 1046, 1931, 2783
BsmAI	GTCTC	4 cut(s)	1121, 1860, 2280, 3185
BsmFI	GGGAC	6 cut(s)	188, 285, 504, 657, 823, 1636
BsnI	GGCC	5 cut(s)	122, 143, 1359, 1482, 1806
Bso31I	GGTCTC	4 cut(s)	1121, 1860, 2280, 3185
BsoBI	CYCGRG	1 cut(s)	3232
Bsp119I	TTCGAA	1 cut(s)	1003
Bsp1286I	GDGCHC	5 cut(s)	1595, 2099, 2319, 3045, 3353
Bsp1407I	TGTACA	2 cut(s)	1071, 1239
Bsp19I	CCATGG	1 cut(s)	1524
BspACI	CCGC	4 cut(s)	478, 572, 3240, 3260
BspANI	GGCC	5 cut(s)	122, 143, 1359, 1482, 1806
BspMAI	CTGCAG	1 cut(s)	3161
BspMI	ACCTGC	1 cut(s)	1161
BspPI	GGATC	3 cut(s)	746, 1100, 1720
BspQI	GCTCTTC	2 cut(s)	57, 3196
BspT104I	TTCGAA	1 cut(s)	1003
BspT107I	GGYRCC	1 cut(s)	2385
BspTNI	GGTCTC	4 cut(s)	1121, 1860, 2280, 3185
BsrDI	GCAATG	1 cut(s)	2934
BsrFI	RCCGGY	3 cut(s)	2870, 3227, 3262
BsrGI	TGTACA	2 cut(s)	1071, 1239
BsrI	ACTGG	4 cut(s)	811, 1789, 2612, 2860
BssAI	RCCGGY	3 cut(s)	2870, 3227, 3262
BssECI	CCNNGG	5 cut(s)	658, 1040, 1524, 1708, 2290
BssT1I	CCWWGG	3 cut(s)	658, 1524, 2290
Bst2UI	CCWGG	3 cut(s)	326, 1041, 1710
Bst6I	CTCTTC	5 cut(s)	57, 423, 1498, 2339, 3196
BstAPI	GCANNNNNTGC	1 cut(s)	86
BstAUI	TGTACA	2 cut(s)	1071, 1239
BstBI	TTCGAA	1 cut(s)	1003
BstC8I	GCNNGC	4 cut(s)	2691, 3135, 3296, 3353
BstDSI	CCRYGG	1 cut(s)	1524
BstF5I	GGATG	5 cut(s)	185, 674, 692, 2714, 3094
BstHHI	GCGC	1 cut(s)	80
BstMAI	GTCTC	4 cut(s)	1121, 1860, 2280, 3185
BstMWI	GCNNNNNNNGC	6 cut(s)	86, 346, 2816, 3237, 3300, 3320
BstNI	CCWGG	3 cut(s)	326, 1041, 1710
BstNSI	RCATGY	2 cut(s)	1057, 1877
BstSCI	CCNGG	5 cut(s)	324, 953, 1039, 1708, 2229
BstSFI	CTRYAG	2 cut(s)	1671, 3157
BstV1I	GCAGC	5 cut(s)	207, 2114, 2188, 3156, 3159
BstV2I	GAAGAC	1 cut(s)	3364
BstX2I	RGATCY	2 cut(s)	485, 784
BstYI	RGATCY	2 cut(s)	485, 784
Bsu36I	CCTNAGG	1 cut(s)	600
BsuRI	GGCC	5 cut(s)	122, 143, 1359, 1482, 1806
BtgI	CCRYGG	1 cut(s)	1524
BtsCI	GGATG	5 cut(s)	185, 674, 692, 2714, 3094
BtsI	GCAGTG	1 cut(s)	1114
BtsIMutI	CAGTG	9 cut(s)	739, 804, 1114, 1347, 1782, 2619, 2620, 2912, 3154
BveI	ACCTGC	1 cut(s)	1161
Cac8I	GCNNGC	4 cut(s)	2691, 3135, 3296, 3353
CaiI	CAGNNNCTG	1 cut(s)	86
CfoI	GCGC	1 cut(s)	80
Cfr10I	RCCGGY	3 cut(s)	2870, 3227, 3262
Cfr13I	GGNCC	8 cut(s)	175, 204, 375, 518, 1628, 1650, 1772, 3257
CseI	GACGC	1 cut(s)	957
CsiI	ACCWGGT	1 cut(s)	324
Csp6I	GTAC	5 cut(s)	720, 1072, 1240, 2945, 3270
CspAI	ACCGGT	1 cut(s)	2870
CviQI	GTAC	5 cut(s)	720, 1072, 1240, 2945, 3270
DraI	TTTAAA	1 cut(s)	3342
EaeI	YGGCCR	1 cut(s)	1480
Eam1104I	CTCTTC	5 cut(s)	57, 423, 1498, 2339, 3196
EarI	CTCTTC	5 cut(s)	57, 423, 1498, 2339, 3196
Eco130I	CCWWGG	3 cut(s)	658, 1524, 2290
Eco147I	AGGCCT	3 cut(s)	122, 143, 1806
Eco24I	GRGCYC	3 cut(s)	2099, 3045, 3353
Eco31I	GGTCTC	4 cut(s)	1121, 1860, 2280, 3185
Eco32I	GATATC	1 cut(s)	1705
Eco47I	GGWCC	8 cut(s)	175, 204, 375, 518, 1628, 1650, 1772, 3257
Eco57I	CTGAAG	2 cut(s)	1623, 3309
Eco81I	CCTNAGG	1 cut(s)	600
Eco88I	CYCGRG	1 cut(s)	3232
EcoO109I	RGGNCCY	3 cut(s)	518, 1650, 1772
EcoRI	GAATTC	1 cut(s)	181
EcoRII	CCWGG	3 cut(s)	324, 1039, 1708
EcoRV	GATATC	1 cut(s)	1705
EcoT14I	CCWWGG	3 cut(s)	658, 1524, 2290
EcoT22I	ATGCAT	4 cut(s)	65, 1693, 2371, 2821
EcoT38I	GRGCYC	3 cut(s)	2099, 3045, 3353
ErhI	CCWWGG	3 cut(s)	658, 1524, 2290
FaqI	GGGAC	6 cut(s)	188, 285, 504, 657, 823, 1636
FauI	CCCGC	1 cut(s)	565
FauNDI	CATATG	1 cut(s)	2081
FbaI	TGATCA	2 cut(s)	2055, 2406
FblI	GTMKAC	1 cut(s)	760
Fnu4HI	GCNGC	5 cut(s)	196, 2128, 2177, 3170, 3173
FokI	GGATG	5 cut(s)	172, 661, 679, 2701, 3101
FriOI	GRGCYC	3 cut(s)	2099, 3045, 3353
Fsp4HI	GCNGC	5 cut(s)	196, 2128, 2177, 3170, 3173
GlaI	GCGC	1 cut(s)	79
GluI	GCNGC	5 cut(s)	196, 2128, 2177, 3170, 3173
GsaI	CCCAGC	2 cut(s)	175, 1049
GsuI	CTGGAG	2 cut(s)	1719, 2832
HaeIII	GGCC	5 cut(s)	122, 143, 1359, 1482, 1806
HapII	CCGG	9 cut(s)	207, 931, 954, 1563, 2231, 2871, 3228, 3263, 3279
HgaI	GACGC	1 cut(s)	957
HhaI	GCGC	1 cut(s)	80
Hin6I	GCGC	1 cut(s)	78
HinP1I	GCGC	1 cut(s)	78
HincII	GTYRAC	2 cut(s)	441, 2896
HindII	GTYRAC	2 cut(s)	441, 2896
HindIII	AAGCTT	4 cut(s)	1732, 2422, 2878, 3131
HpaI	GTTAAC	2 cut(s)	441, 2896
HpaII	CCGG	9 cut(s)	207, 931, 954, 1563, 2231, 2871, 3228, 3263, 3279
HphI	GGTGA	8 cut(s)	340, 893, 976, 1349, 1577, 2401, 2885, 3049
Hpy99I	CGWCG	1 cut(s)	2211
HpyCH4IV	ACGT	1 cut(s)	2160
HpyF10VI	GCNNNNNNNGC	6 cut(s)	86, 346, 2816, 3237, 3300, 3320
HpySE526I	ACGT	1 cut(s)	2160
HspAI	GCGC	1 cut(s)	78
KflI	GGGWCCC	1 cut(s)	1650
Ksp22I	TGATCA	2 cut(s)	2055, 2406
KspAI	GTTAAC	2 cut(s)	441, 2896
LguI	GCTCTTC	2 cut(s)	57, 3196
LmnI	GCTCC	9 cut(s)	446, 506, 600, 823, 1850, 2253, 2481, 2741, 2845
Lsp1109I	GCAGC	5 cut(s)	207, 2114, 2188, 3156, 3159
LweI	GCATC	4 cut(s)	2188, 2723, 2806, 3079
MabI	ACCWGGT	1 cut(s)	324
MaeII	ACGT	1 cut(s)	2160
MaeIII	GTNAC	7 cut(s)	395, 794, 1265, 1666, 1942, 2697, 2961
MfeI	CAATTG	3 cut(s)	199, 1361, 2562
MflI	RGATCY	2 cut(s)	485, 784
MhlI	GDGCHC	5 cut(s)	1595, 2099, 2319, 3045, 3353
MlyI	GAGTC	5 cut(s)	92, 244, 1200, 2945, 3256
MmeI	TCCRAC	5 cut(s)	528, 2224, 2656, 2823, 2891
Mph1103I	ATGCAT	4 cut(s)	65, 1693, 2371, 2821
MroXI	GAANNNNTTC	6 cut(s)	909, 1558, 2202, 2348, 2515, 3241
MslI	CAYNNNNRTG	1 cut(s)	1293
MspA1I	CMGCKG	2 cut(s)	1837, 2810
MspI	CCGG	9 cut(s)	207, 931, 954, 1563, 2231, 2871, 3228, 3263, 3279
MspR9I	CCNGG	5 cut(s)	326, 955, 1041, 1710, 2231
MunI	CAATTG	3 cut(s)	199, 1361, 2562
MvaI	CCWGG	3 cut(s)	326, 1041, 1710
MwoI	GCNNNNNNNGC	6 cut(s)	86, 346, 2816, 3237, 3300, 3320
NciI	CCSGG	2 cut(s)	955, 2231
NcoI	CCATGG	1 cut(s)	1524
NdeI	CATATG	1 cut(s)	2081
NmeAIII	GCCGAG	1 cut(s)	3196
NmuCI	GTSAC	1 cut(s)	1666
NsiI	ATGCAT	4 cut(s)	65, 1693, 2371, 2821
NspI	RCATGY	2 cut(s)	1057, 1877
NspV	TTCGAA	1 cut(s)	1003
PceI	AGGCCT	3 cut(s)	122, 143, 1806
PciSI	GCTCTTC	2 cut(s)	57, 3196
PcsI	WCGNNNNNNNCGW	2 cut(s)	1000, 3307
PdmI	GAANNNNTTC	6 cut(s)	909, 1558, 2202, 2348, 2515, 3241
PfeI	GAWTC	8 cut(s)	5, 637, 886, 991, 1202, 1252, 1468, 1921
PflMI	CCANNNNNTGG	1 cut(s)	2783
PinAI	ACCGGT	1 cut(s)	2870
PkrI	GCNGC	5 cut(s)	197, 2129, 2178, 3171, 3174
PleI	GAGTC	5 cut(s)	91, 243, 1199, 2945, 3255
PpsI	GAGTC	5 cut(s)	91, 243, 1199, 2945, 3255
PpuMI	RGGWCCY	3 cut(s)	518, 1650, 1772
PsiI	TTATAA	1 cut(s)	2271
Psp5II	RGGWCCY	3 cut(s)	518, 1650, 1772
Psp6I	CCWGG	3 cut(s)	324, 1039, 1708
PspFI	CCCAGC	2 cut(s)	171, 1045
PspGI	CCWGG	3 cut(s)	324, 1039, 1708
PspPI	GGNCC	8 cut(s)	175, 204, 375, 518, 1628, 1650, 1772, 3257
PspPPI	RGGWCCY	3 cut(s)	518, 1650, 1772
PstI	CTGCAG	1 cut(s)	3161
PstNI	CAGNNNCTG	1 cut(s)	86
PsuI	RGATCY	2 cut(s)	485, 784
PvuII	CAGCTG	2 cut(s)	1837, 2810
RsaI	GTAC	5 cut(s)	721, 1073, 1241, 2946, 3271
RsaNI	GTAC	5 cut(s)	720, 1072, 1240, 2945, 3270
RseI	CAYNNNNRTG	1 cut(s)	1293
SapI	GCTCTTC	2 cut(s)	57, 3196
SatI	GCNGC	5 cut(s)	196, 2128, 2177, 3170, 3173
Sau96I	GGNCC	8 cut(s)	175, 204, 375, 518, 1628, 1650, 1772, 3257
ScaI	AGTACT	1 cut(s)	2946
SchI	GAGTC	5 cut(s)	92, 244, 1200, 2945, 3256
ScrFI	CCNGG	5 cut(s)	326, 955, 1041, 1710, 2231
SduI	GDGCHC	5 cut(s)	1595, 2099, 2319, 3045, 3353
SexAI	ACCWGGT	1 cut(s)	324
SfaNI	GCATC	4 cut(s)	2188, 2723, 2806, 3079
SfcI	CTRYAG	2 cut(s)	1671, 3157
SfuI	TTCGAA	1 cut(s)	1003
SgrAI	CRCCGGYG	1 cut(s)	3262
SinI	GGWCC	8 cut(s)	175, 204, 375, 518, 1628, 1650, 1772, 3257
SmiMI	CAYNNNNRTG	1 cut(s)	1293
SmlI	CTYRAG	5 cut(s)	481, 773, 2419, 2750, 3082
SmoI	CTYRAG	5 cut(s)	481, 773, 2419, 2750, 3082
SpeI	ACTAGT	1 cut(s)	2041
SseBI	AGGCCT	3 cut(s)	122, 143, 1806
SsiI	CCGC	4 cut(s)	478, 572, 3240, 3260
SspI	AATATT	2 cut(s)	289, 898
StuI	AGGCCT	3 cut(s)	122, 143, 1806
StyD4I	CCNGG	5 cut(s)	324, 953, 1039, 1708, 2229
StyI	CCWWGG	3 cut(s)	658, 1524, 2290
TaiI	ACGT	1 cut(s)	2163
TatI	WGTACW	3 cut(s)	1071, 1239, 2944
TfiI	GAWTC	8 cut(s)	5, 637, 886, 991, 1202, 1252, 1468, 1921
TscAI	CASTG	9 cut(s)	739, 811, 1114, 1354, 1789, 2619, 2627, 2912, 3154
TseFI	GTSAC	1 cut(s)	1666
TseI	GCWGC	5 cut(s)	195, 2127, 2176, 3169, 3172
Tsp45I	GTSAC	1 cut(s)	1666
TspDTI	ATGAA	9 cut(s)	174, 663, 831, 918, 1413, 1952, 2012, 2211, 2872
TspGWI	ACGGA	2 cut(s)	1566, 3088
TspRI	CASTG	9 cut(s)	739, 811, 1114, 1354, 1789, 2619, 2627, 2912, 3154
Van91I	CCANNNNNTGG	1 cut(s)	2783
VpaK11BI	GGWCC	8 cut(s)	175, 204, 375, 518, 1628, 1650, 1772, 3257
XbaI	TCTAGA	1 cut(s)	1816
XceI	RCATGY	2 cut(s)	1057, 1877
XcmI	CCANNNNNNNNNTGG	1 cut(s)	168
XmiI	GTMKAC	1 cut(s)	760
XmnI	GAANNNNTTC	6 cut(s)	909, 1558, 2202, 2348, 2515, 3241
ZrmI	AGTACT	1 cut(s)	2946
Zsp2I	ATGCAT	4 cut(s)	65, 1693, 2371, 2821

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.