Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005533

Physical Location & Seq

Genomic Coordinates Forward (+)

5101927 .. 5105142

Gene Structure 3216 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1074900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3018 bp

ATGGATTTCCACTTCCATCTATTGCCACAACTTTCCACATTTTCTATTCTTCTGATATGCTTTTTTGTGACCATAGAGTCAGTAATTCCAAGCATTTCCACAGACAAAGAGGCCTTGATCTCATTCAAATCAACGGCCAATCTCCCTCCATATTTCTGGGACCAAAGCTCATCCCCCTGCACCAACTGGACTGGAGTTGTATGCAACAAACATGGCAAGAGAGTCATTGCTCTTGACCTCTCAGGCTTGGGACTCACAGGCTCCATAAGCCCTCACATTGGTAACCTCTCCTTCCTCCATTCCCTCCAACTCCATAACAACAAATTAACCGGAACAATTCCCACTCAAATTTTCAACCTGTTTCGCTTGAGGTCACTCAACGTAAGCTCCAACACCCTACGAGGTTCACTTCCACCAAACATAACCCAATTGATGGCACTCGAAATACTTGACTTGTCCTCAAACAACATCACAGGAATCCTCCCGCAAAACCTTGGACAGTTGAAAAATCTCCAAGTCCTGAATTTGGCACGAAACAATTTTTTCGGTCCGATCCCACCATCGATTAGCAACCTTTCATCCACCCTCACAAATTTGAATCTAGGTACAAACAGACTCAGTGGTACTATACCTAGTGAATTAGGTCACCTCCATAAGCTCAAAGAACTCGACCTCGCCGTTAACCAACTCACCGGGACCATTGCCTCATCCATATACAACATTTCCTCTCTAGTTTTGTTCACTGTGGCTTCAAACCATCTGTGGGGTCAGATTCCTAGCAATATTGGTGACACACTTCCAAATCTTTTGTACTTCAGGAATTGTATCAATCAATTCACAGGAAAAATTCCTCCTTCTCTACACAACATCTCTGGACTTCGATCAATTCGCATGTCTAACAACTTTCTTGAAGGAACAGTTCCACCAGGCCTCGGAAATCTTCCCTTCCTGGAAATGTACAACATTGGCTTCAATCGGATAGTTAGCTACGGTGACAATGGTCTTAATTTCATTACTACTTTGACAAACAGCAGTCGCTTAAGTTGGCTTGCCATTGATGAAAATCGGCTGGAAGGCCTAATTCCAGAGTCCATTGGCAACCTCTCCAAGGTTCTGTCAAAATTGTACATGGGAGGGAATCGAATTTACGGTAACATACCTGCCTCAATTAGTCAACTCAGTAGTTTAACTTTGCTAAATGTGAGCTACAATTCAATTTCTGGTGAAATCCCATCAGAAATTGGGCAGCTGAAAGAGTTGCAAATGCTTGGTTTAGCAAGAAATAAATTGTCTGGCACAATTCCAAACTCCTTAGGTAATATCATGAAATTGAACCATATTGATTTATCTGGAAACTATTTTGTTGGCCATATACCCCCTTCATTTTCAAACTTCCAGAAATTGCTTTCCATAGACTTATCTAACAATTCGCTCAACGGAAGCATTTCCGGAGAAATATTTCTAAACCTTCCTAGTTTGAGCACTATTCTCAATCTGTCTAACAATTTCCTGAGTGGAGCTTTGCCTGAAGAATTAGGATCTCTAGCAAGTGTTGCCACTATTGACCTCTCTAGCAACCAGTTTTCGGGGTTGATTCCCAGCTCAATTGGAAAGTGTACAAGCTTGGAAGGATTGTTCATGGCCAGAAACAAACTTTCAGGCCCCCTTCCAAATGCTCTTGGAGAAATGAGAGGTCTTGAGATTTTAGACCTCTCCTCGAACCAACTATCAGGCTTTATTCCTGATAAACTTCAAGACCTACGTGTCCTTCGATACTTGAACCTCTCATTTAATAACTTAGAAGGAGTGATTCCAAATGGTGGAGTTTTTAAAAATATCTCTAGTGTCCATCTTGAAGGCAACCCTAAGCTATGCATGCATTTCCCATGTGTGAAACGTAGGAGAAAGGTACTTGTTCGAGTTCTTATCACTACTGTTGCCTTGGCAACGTTGGCAACATGTATTACAGTTGGTCTTTTGCTACGTTTTAGAAAAAGAAGAGGCTCAGGCGGCAAAGCAACAGAAACTAATTCTGGTTTGCTACTAAAAGGGCAACATCAAATGGTCAGTTACGAAGAGCTACGTGGGGCAACTGGGAATTTCAACCCCGAAAATTTGATTGGAACAGGGAGCTTTGGGGCTGTATACAAAGGTCTTTTGAGAGAAGGAACTGAGGTTGCAGTGAAGGTGCTTGATATGAAAATGACAGCATCTTGGAAGAGTTTTATGGCAGAGTGCGAAGCATTGAGGAGCGTGAGGCACCGGAATCTTGTTAAACTGATCACCTCTTGCTCCAGCTTGGACTTTAAAAACATGGAGTTTCTTGCTTTGGTTTACGAGTTTTTGAGTAATGGGAGCTTGGAAGATTGGATTAGAGGCAAGAGAAAAAATGCAGATGGAGATGGGTTGAACATTTTTGAGAGACTAAATGTGGCCATTGATGTGGCGTGTGGATTGGATTATCTGCATCATGACTGCGAAGTTCCGGTTGTGCACTGCGATTTAAAGCCGAGCAACATTCTTTTGGATGGAGATATGAATGCAAAGATCGGAGATTTCGGGCTGGCGAAGCTGCTGATGGAGAGTACAGTGATTGCGCAACAGTCACTTAGCTCCACACATGTCCTGAAGGGTTCTATAGGATACATACCCCCTGAGTATGGTATCCACCAGAAACCCTCGACAGCGGGTGATGCATACAGTTTCGGTGTAGTGTTGTTACAGCTCTTCACCGGGAAAAGCCCCACCCATGAAAGTTTCACAGGAGATCAAAGCATCATCACATGGGTGCAGTCAGCCTTCCCTCACGACGTGATCAAAGTGCTAGATTCCGAGCTGCTAGACGATCTCATCATCACAGAAGTAGAACATAATTGCCTCATCTCAATCATTGAGGTTGGCCTCTCTTGCACGTGTGCTTCTCGCGACGGACGCATAAGCTTGAGGGACGCCCTTCACAAATTGGAAACTGCCAGAAAGACCCTTCTCAGCGCAAAGAGTGCCAAGCATCAATATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009987 GO:0010204 GO:0010359 GO:0012501 GO:0012505 GO:0016020 GO:0016045 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031347 GO:0031349 GO:0032409 GO:0032412 GO:0032879 GO:0033554 GO:0034050 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080134 GO:0098543 GO:0098581 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1006

Amino Acids

109.62

Weight (kDa)

8.34

Isoelectric Point (pI)

40.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	33 - 69	1.8e-07	Leucine rich repeat N-terminal domain
LRR_14	PF23598	89 - 241	2.4e-14	Leucine-rich repeat region
LRR_4	PF12799	147 - 187	4e-06	Leucine Rich repeats (2 copies)
LRR_14	PF23598	339 - 449	3.9e-08	Leucine-rich repeat region
LRR_14	PF23598	460 - 599	1.1e-10	Leucine-rich repeat region
LRR_8	PF13855	560 - 600	7.8e-08	Leucine rich repeat
Pkinase	PF00069	703 - 920	1e-38	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	705 - 919	2.1e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g36850 FvH4_3g36850 FvH4_3g36850
malus_domestica	MD08G1090900.v1.1 MD15G1074900.v1.1 MD15G1075000.v1.1
prunus_persica	Prupe.1G427400_v2.0.a1 Prupe.1G427700_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1
pyrus_communis	pycom15g06980 pycom15g06990
rosa_chinensis	RchiOBHm_Chr5g0066001 RchiOBHm_Chr5g0066021
rosa_laevigata	RLG00000035833 RLG00000035834
rosa_multiflora	Rmu_sc0001473.1_g000006 Rmu_sc0011576.1_g000012 Rmu_sc0011576.1_g000015
rosa_roxburghii	Rroxscaffold_1G00014980 Rroxscaffold_1G00015120
rosa_rugosa	Rorug05G0375800 Rorug05G0375900 Rorug05G0376000
rosa_samantha	Rh5AG434300 Rh5AG434500 Rh5CG472900
rosa_wichuraiana	Rw5G040690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	76, 1763
Acc16I	TGCGCA	1 cut(s)	2598
Acc36I	ACCTGC	1 cut(s)	1168
AccB1I	GGYRCC	1 cut(s)	2259
AccB7I	CCANNNNNTGG	2 cut(s)	433, 1820
AccI	GTMKAC	1 cut(s)	2145
AccII	CGCG	1 cut(s)	2925
AccIII	TCCGGA	1 cut(s)	1448
AciI	CCGC	3 cut(s)	485, 2010, 2687
AclI	AACGTT	1 cut(s)	1949
AclWI	GGATC	2 cut(s)	547, 1546
AcoI	YGGCCR	4 cut(s)	135, 1366, 1641, 2433
AcsI	RAATTY	7 cut(s)	348, 523, 592, 846, 1143, 2098, 2113
AcuI	CTGAAG	3 cut(s)	799, 1548, 2648
AcvI	CACGTG	1 cut(s)	2913
AcyI	GRCGYC	1 cut(s)	2949
AdeI	CACNNNGTG	1 cut(s)	2812
AfaI	GTAC	8 cut(s)	607, 625, 812, 959, 1127, 1618, 1911, 2587
AfiI	CCNNNNNNNGG	9 cut(s)	278, 433, 526, 763, 932, 1108, 1585, 1820, 2660
AflII	CTTAAG	1 cut(s)	1039
AflIII	ACRYGT	4 cut(s)	1762, 1958, 2620, 2912
AjiI	CACGTC	1 cut(s)	2812
AjnI	CCWGG	2 cut(s)	925, 948
AjuI	GAANNNNNNNTTGG	2 cut(s)	2103, 2135
AleI	CACNNNNGTG	1 cut(s)	2786
AloI	GAACNNNNNNTCC	2 cut(s)	1619, 1651
Alw21I	GWGCWC	2 cut(s)	1484, 2496
Alw26I	GTCTC	1 cut(s)	2416
Alw44I	GTGCAC	1 cut(s)	2492
AlwI	GGATC	2 cut(s)	547, 1546
Aor13HI	TCCGGA	1 cut(s)	1448
AoxI	GGCC	9 cut(s)	111, 135, 928, 1075, 1366, 1641, 1660, 2433, 2899
ApaLI	GTGCAC	1 cut(s)	2492
ApeKI	GCWGC	3 cut(s)	1246, 2572, 2836
ApoI	RAATTY	7 cut(s)	348, 523, 592, 846, 1143, 2098, 2113
Asp700I	GAANNNNTTC	2 cut(s)	1444, 1458
AspLEI	GCGC	2 cut(s)	2599, 2993
AspS9I	GGNCC	4 cut(s)	160, 548, 696, 1661
AsuC2I	CCSGG	2 cut(s)	694, 2734
AsuHPI	GGTGA	8 cut(s)	638, 682, 800, 1004, 1235, 2275, 2702, 2722
AvaII	GGWCC	3 cut(s)	160, 548, 696
AxyI	CCTNAGG	1 cut(s)	1312
BaeGI	GKGCMC	1 cut(s)	2496
BalI	TGGCCA	3 cut(s)	1368, 1643, 2435
BanI	GGYRCC	1 cut(s)	2259
BbrPI	CACGTG	1 cut(s)	2913
Bbv12I	GWGCWC	2 cut(s)	1484, 2496
BbvI	GCAGC	3 cut(s)	1258, 2559, 2823
BceAI	ACGGC	2 cut(s)	150, 662
BciT130I	CCWGG	2 cut(s)	927, 950
BciVI	GTATCC	2 cut(s)	2636, 2675
BclI	TGATCA	2 cut(s)	2280, 2814
BcnI	CCSGG	2 cut(s)	694, 2734
BcoDI	GTCTC	1 cut(s)	2416
BfmI	CTRYAG	1 cut(s)	2637
BfrI	CTTAAG	1 cut(s)	1039
BfuAI	ACCTGC	1 cut(s)	1168
BfuI	GTATCC	2 cut(s)	2636, 2675
BisI	GCNGC	4 cut(s)	1247, 2011, 2573, 2837
BlsI	GCNGC	4 cut(s)	1248, 2012, 2574, 2838
Bme1390I	CCNGG	4 cut(s)	694, 927, 950, 2734
Bme18I	GGWCC	3 cut(s)	160, 548, 696
BmgBI	CACGTC	1 cut(s)	2812
BmgT120I	GGNCC	4 cut(s)	160, 548, 696, 1661
BmiI	GGNNCC	5 cut(s)	161, 262, 697, 1663, 2261
BmrFI	CCNGG	4 cut(s)	694, 927, 950, 2734
BmrI	ACTGGG	1 cut(s)	2103
BmsI	GCATC	4 cut(s)	2219, 2476, 2683, 2784
BmuI	ACTGGG	1 cut(s)	2103
BoxI	GACNNNNGTC	1 cut(s)	999
BpmI	CTGGAG	2 cut(s)	213, 2278
Bpu10I	CCTNAGC	2 cut(s)	1866, 2005
BpuEI	CTTGAG	3 cut(s)	388, 1718, 2962
BpuMI	CCSGG	2 cut(s)	694, 2734
Bsa29I	ATCGAT	1 cut(s)	563
BsaAI	YACGTR	3 cut(s)	1763, 2084, 2913
BsaBI	GATNNNNATC	1 cut(s)	1062
BsaHI	GRCGYC	1 cut(s)	2949
BsaJI	CCNNGG	4 cut(s)	493, 931, 1107, 1941
BsaWI	WCCGGW	4 cut(s)	329, 1448, 2262, 2485
BsaXI	ACNNNNNCTCC	2 cut(s)	371, 401
Bsc4I	CCNNNNNNNGG	9 cut(s)	278, 433, 526, 763, 932, 1108, 1585, 1820, 2660
Bse1I	ACTGG	4 cut(s)	191, 196, 1579, 2098
Bse21I	CCTNAGG	1 cut(s)	1312
Bse3DI	GCAATG	2 cut(s)	225, 699
Bse8I	GATNNNNATC	1 cut(s)	1062
BseAI	TCCGGA	1 cut(s)	1448
BseBI	CCWGG	2 cut(s)	927, 950
BseCI	ATCGAT	1 cut(s)	563
BseDI	CCNNGG	4 cut(s)	493, 931, 1107, 1941
BseGI	GGATG	4 cut(s)	170, 578, 707, 2533
BseJI	GATNNNNATC	1 cut(s)	1062
BseLI	CCNNNNNNNGG	9 cut(s)	278, 433, 526, 763, 932, 1108, 1585, 1820, 2660
BseMI	GCAATG	2 cut(s)	225, 699
BseMII	CTCAG	8 cut(s)	255, 631, 1192, 1502, 2019, 2163, 2646, 3001
BseNI	ACTGG	4 cut(s)	191, 196, 1579, 2098
BseRI	GAGGAG	2 cut(s)	1705, 2263
BseSI	GKGCMC	1 cut(s)	2496
BseXI	GCAGC	3 cut(s)	1258, 2559, 2823
BseYI	CCCAGC	1 cut(s)	1598
BsgI	GTGCAG	2 cut(s)	163, 2810
Bsh1236I	CGCG	1 cut(s)	2925
BshFI	GGCC	9 cut(s)	113, 137, 930, 1077, 1368, 1643, 1662, 2435, 2901
BshNI	GGYRCC	1 cut(s)	2259
BshVI	ATCGAT	1 cut(s)	563
BsiHKAI	GWGCWC	2 cut(s)	1484, 2496
BsiSI	CCGG	6 cut(s)	330, 693, 1449, 2263, 2486, 2733
BslFI	GGGAC	4 cut(s)	173, 264, 709, 2960
BslI	CCNNNNNNNGG	9 cut(s)	278, 433, 526, 763, 932, 1108, 1585, 1820, 2660
BsmAI	GTCTC	1 cut(s)	2416
BsmFI	GGGAC	4 cut(s)	173, 264, 709, 2960
BsmI	GAATGC	1 cut(s)	2545
BsnI	GGCC	9 cut(s)	113, 137, 930, 1077, 1368, 1643, 1662, 2435, 2901
Bsp1286I	GDGCHC	2 cut(s)	1484, 2496
Bsp13I	TCCGGA	1 cut(s)	1448
Bsp1407I	TGTACA	3 cut(s)	957, 1125, 1616
Bsp143I	GATC	9 cut(s)	117, 552, 881, 1538, 2280, 2547, 2767, 2814, 2845
Bsp68I	TCGCGA	1 cut(s)	2925
BspACI	CCGC	3 cut(s)	485, 2010, 2687
BspANI	GGCC	9 cut(s)	113, 137, 930, 1077, 1368, 1643, 1662, 2435, 2901
BspCNI	CTCAG	8 cut(s)	254, 630, 1191, 1503, 2018, 2164, 2647, 3000
BspDI	ATCGAT	1 cut(s)	563
BspEI	TCCGGA	1 cut(s)	1448
BspFNI	CGCG	1 cut(s)	2925
BspHI	TCATGA	2 cut(s)	1323, 2470
BspLI	GGNNCC	5 cut(s)	161, 262, 697, 1663, 2261
BspMI	ACCTGC	1 cut(s)	1168
BspPI	GGATC	2 cut(s)	547, 1546
BspQI	GCTCTTC	2 cut(s)	2070, 2732
BspT107I	GGYRCC	1 cut(s)	2259
BspTI	CTTAAG	1 cut(s)	1039
BsrDI	GCAATG	2 cut(s)	225, 699
BsrGI	TGTACA	3 cut(s)	957, 1125, 1616
BsrI	ACTGG	4 cut(s)	191, 196, 1579, 2098
BssECI	CCNNGG	4 cut(s)	493, 931, 1107, 1941
BssMI	GATC	9 cut(s)	117, 552, 881, 1538, 2280, 2547, 2767, 2814, 2845
BssNAI	GTATAC	1 cut(s)	2146
BssNI	GRCGYC	1 cut(s)	2949
BssT1I	CCWWGG	3 cut(s)	493, 1107, 1941
Bst1107I	GTATAC	1 cut(s)	2146
Bst2UI	CCWGG	2 cut(s)	927, 950
Bst6I	CTCTTC	4 cut(s)	1993, 2070, 2213, 2732
BstACI	GRCGYC	1 cut(s)	2949
BstAFI	CTTAAG	1 cut(s)	1039
BstAPI	GCANNNNNTGC	2 cut(s)	1553, 2999
BstAUI	TGTACA	3 cut(s)	957, 1125, 1616
BstBAI	YACGTR	3 cut(s)	1763, 2084, 2913
BstC8I	GCNNGC	3 cut(s)	1050, 1877, 2565
BstEII	GGTNACC	2 cut(s)	281, 644
BstENI	CCTNNNNNAGG	1 cut(s)	1106
BstF5I	GGATG	4 cut(s)	170, 578, 707, 2533
BstFNI	CGCG	1 cut(s)	2925
BstHHI	GCGC	2 cut(s)	2599, 2993
BstKTI	GATC	9 cut(s)	120, 555, 884, 1541, 2283, 2550, 2770, 2817, 2848
BstMAI	GTCTC	1 cut(s)	2416
BstMBI	GATC	9 cut(s)	117, 552, 881, 1538, 2280, 2547, 2767, 2814, 2845
BstNI	CCWGG	2 cut(s)	927, 950
BstNSI	RCATGY	4 cut(s)	895, 1879, 1962, 2624
BstPAI	GACNNNNGTC	1 cut(s)	999
BstPI	GGTNACC	2 cut(s)	281, 644
BstSCI	CCNGG	4 cut(s)	692, 925, 948, 2732
BstSFI	CTRYAG	1 cut(s)	2637
BstSLI	GKGCMC	1 cut(s)	2496
BstUI	CGCG	1 cut(s)	2925
BstV1I	GCAGC	3 cut(s)	1258, 2559, 2823
BstX2I	RGATCY	1 cut(s)	1538
BstXI	CCANNNNNNTGG	2 cut(s)	156, 2443
BstYI	RGATCY	1 cut(s)	1538
BstZ17I	GTATAC	1 cut(s)	2146
Bsu15I	ATCGAT	1 cut(s)	563
Bsu36I	CCTNAGG	1 cut(s)	1312
BsuI	GTATCC	2 cut(s)	2636, 2675
BsuRI	GGCC	9 cut(s)	113, 137, 930, 1077, 1368, 1643, 1662, 2435, 2901
BsuTUI	ATCGAT	1 cut(s)	563
BtrI	CACGTC	1 cut(s)	2812
BtsCI	GGATG	4 cut(s)	170, 578, 707, 2533
BtsI	GCAGTG	2 cut(s)	2187, 2494
BtsIMutI	CAGTG	5 cut(s)	625, 741, 2187, 2494, 2595
BtuMI	TCGCGA	1 cut(s)	2925
BveI	ACCTGC	1 cut(s)	1168
Cac8I	GCNNGC	3 cut(s)	1050, 1877, 2565
CciI	TCATGA	2 cut(s)	1323, 2470
CfoI	GCGC	2 cut(s)	2599, 2993
Cfr13I	GGNCC	4 cut(s)	160, 548, 696, 1661
ClaI	ATCGAT	1 cut(s)	563
CpoI	CGGWCCG	1 cut(s)	548
CseI	GACGC	2 cut(s)	2940, 2957
Csp6I	GTAC	8 cut(s)	606, 624, 811, 958, 1126, 1617, 1910, 2586
CspI	CGGWCCG	1 cut(s)	548
CviQI	GTAC	8 cut(s)	606, 624, 811, 958, 1126, 1617, 1910, 2586
DpnI	GATC	9 cut(s)	119, 554, 883, 1540, 2282, 2549, 2769, 2816, 2847
DpnII	GATC	9 cut(s)	117, 552, 881, 1538, 2280, 2547, 2767, 2814, 2845
DraI	TTTAAA	3 cut(s)	1831, 2308, 2505
DraIII	CACNNNGTG	1 cut(s)	2812
DrdI	GACNNNNNNGTC	2 cut(s)	76, 1763
DseDI	GACNNNNNNGTC	2 cut(s)	76, 1763
EaeI	YGGCCR	4 cut(s)	135, 1366, 1641, 2433
Eam1104I	CTCTTC	4 cut(s)	1993, 2070, 2213, 2732
EarI	CTCTTC	4 cut(s)	1993, 2070, 2213, 2732
Eco130I	CCWWGG	3 cut(s)	493, 1107, 1941
Eco147I	AGGCCT	3 cut(s)	113, 930, 1077
Eco47I	GGWCC	3 cut(s)	160, 548, 696
Eco57I	CTGAAG	3 cut(s)	799, 1548, 2648
Eco72I	CACGTG	1 cut(s)	2913
Eco81I	CCTNAGG	1 cut(s)	1312
Eco91I	GGTNACC	2 cut(s)	281, 644
EcoNI	CCTNNNNNAGG	1 cut(s)	1106
EcoO109I	RGGNCCY	1 cut(s)	1661
EcoO65I	GGTNACC	2 cut(s)	281, 644
EcoRII	CCWGG	2 cut(s)	925, 948
EcoT14I	CCWWGG	3 cut(s)	493, 1107, 1941
EcoT22I	ATGCAT	3 cut(s)	1877, 1881, 2698
ErhI	CCWWGG	3 cut(s)	493, 1107, 1941
FaqI	GGGAC	4 cut(s)	173, 264, 709, 2960
FauI	CCCGC	2 cut(s)	492, 2680
FbaI	TGATCA	2 cut(s)	2280, 2814
FblI	GTMKAC	1 cut(s)	2145
Fnu4HI	GCNGC	4 cut(s)	1247, 2011, 2573, 2837
FokI	GGATG	4 cut(s)	157, 565, 694, 2540
Fsp4HI	GCNGC	4 cut(s)	1247, 2011, 2573, 2837
FspI	TGCGCA	1 cut(s)	2598
GlaI	GCGC	2 cut(s)	2598, 2992
GluI	GCNGC	4 cut(s)	1247, 2011, 2573, 2837
GsaI	CCCAGC	1 cut(s)	1602
GsuI	CTGGAG	2 cut(s)	213, 2278
HaeIII	GGCC	9 cut(s)	113, 137, 930, 1077, 1368, 1643, 1662, 2435, 2901
HapII	CCGG	6 cut(s)	330, 693, 1449, 2263, 2486, 2733
HgaI	GACGC	2 cut(s)	2940, 2957
HhaI	GCGC	2 cut(s)	2599, 2993
Hin1I	GRCGYC	1 cut(s)	2949
Hin6I	GCGC	2 cut(s)	2597, 2991
HinP1I	GCGC	2 cut(s)	2597, 2991
HincII	GTYRAC	2 cut(s)	682, 1175
HindII	GTYRAC	2 cut(s)	682, 1175
HindIII	AAGCTT	2 cut(s)	1621, 2938
HpaI	GTTAAC	1 cut(s)	682
HpaII	CCGG	6 cut(s)	330, 693, 1449, 2263, 2486, 2733
HphI	GGTGA	8 cut(s)	638, 682, 800, 1004, 1235, 2275, 2702, 2722
Hpy166II	GTNNAC	8 cut(s)	407, 682, 741, 1175, 1617, 2146, 2335, 2494
Hpy188I	TCNGA	8 cut(s)	54, 552, 771, 935, 978, 1237, 2552, 2833
Hpy8I	GTNNAC	8 cut(s)	407, 682, 741, 1175, 1617, 2146, 2335, 2494
Hpy99I	CGWCG	2 cut(s)	2813, 2930
HpyCH4IV	ACGT	8 cut(s)	381, 1762, 1897, 1949, 1984, 2083, 2811, 2912
HpySE526I	ACGT	8 cut(s)	381, 1762, 1897, 1949, 1984, 2083, 2811, 2912
Hsp92I	GRCGYC	1 cut(s)	2949
HspAI	GCGC	2 cut(s)	2597, 2991
Kpn2I	TCCGGA	1 cut(s)	1448
Ksp22I	TGATCA	2 cut(s)	2280, 2814
KspAI	GTTAAC	1 cut(s)	682
Kzo9I	GATC	9 cut(s)	117, 552, 881, 1538, 2280, 2547, 2767, 2814, 2845
LguI	GCTCTTC	2 cut(s)	2070, 2732
LmnI	GCTCC	8 cut(s)	266, 392, 1517, 2130, 2250, 2297, 2355, 2618
Lsp1109I	GCAGC	3 cut(s)	1258, 2559, 2823
LweI	GCATC	4 cut(s)	2219, 2476, 2683, 2784
MaeII	ACGT	8 cut(s)	381, 1762, 1897, 1949, 1984, 2083, 2811, 2912
MalI	GATC	9 cut(s)	119, 554, 883, 1540, 2282, 2549, 2769, 2816, 2847
MboI	GATC	9 cut(s)	117, 552, 881, 1538, 2280, 2547, 2767, 2814, 2845
MboII	GAAGA	8 cut(s)	41, 932, 1541, 2010, 2087, 2230, 2375, 2719
MfeI	CAATTG	2 cut(s)	428, 1605
MflI	RGATCY	1 cut(s)	1538
MhlI	GDGCHC	2 cut(s)	1484, 2496
MlsI	TGGCCA	3 cut(s)	1368, 1643, 2435
MluNI	TGGCCA	3 cut(s)	1368, 1643, 2435
MlyI	GAGTC	5 cut(s)	86, 231, 246, 609, 1097
MmeI	TCCRAC	2 cut(s)	331, 414
Mox20I	TGGCCA	3 cut(s)	1368, 1643, 2435
Mph1103I	ATGCAT	3 cut(s)	1877, 1881, 2698
MroI	TCCGGA	1 cut(s)	1448
MroXI	GAANNNNTTC	2 cut(s)	1444, 1458
MscI	TGGCCA	3 cut(s)	1368, 1643, 2435
MslI	CAYNNNNRTG	2 cut(s)	2441, 2786
Msp20I	TGGCCA	3 cut(s)	1368, 1643, 2435
MspA1I	CMGCKG	2 cut(s)	1249, 2687
MspCI	CTTAAG	1 cut(s)	1039
MspI	CCGG	6 cut(s)	330, 693, 1449, 2263, 2486, 2733
MspR9I	CCNGG	4 cut(s)	694, 927, 950, 2734
MunI	CAATTG	2 cut(s)	428, 1605
Mva1269I	GAATGC	1 cut(s)	2545
MvaI	CCWGG	2 cut(s)	927, 950
MvnI	CGCG	1 cut(s)	2925
NciI	CCSGG	2 cut(s)	694, 2734
NdeII	GATC	9 cut(s)	117, 552, 881, 1538, 2280, 2547, 2767, 2814, 2845
NlaIV	GGNNCC	5 cut(s)	161, 262, 697, 1663, 2261
NmeAIII	GCCGAG	1 cut(s)	2535
NmuCI	GTSAC	6 cut(s)	67, 372, 644, 788, 992, 2604
NruI	TCGCGA	1 cut(s)	2925
NsbI	TGCGCA	1 cut(s)	2598
NsiI	ATGCAT	3 cut(s)	1877, 1881, 2698
NspI	RCATGY	4 cut(s)	895, 1879, 1962, 2624
OliI	CACNNNNGTG	1 cut(s)	2786
PaeI	GCATGC	1 cut(s)	1879
PagI	TCATGA	2 cut(s)	1323, 2470
PceI	AGGCCT	3 cut(s)	113, 930, 1077
PciI	ACATGT	2 cut(s)	1958, 2620
PciSI	GCTCTTC	2 cut(s)	2070, 2732
PcsI	WCGNNNNNNNCGW	2 cut(s)	675, 1768
PctI	GAATGC	1 cut(s)	2545
PdmI	GAANNNNTTC	2 cut(s)	1444, 1458
PfeI	GAWTC	8 cut(s)	477, 598, 772, 1138, 1594, 1810, 2266, 2828
PflMI	CCANNNNNTGG	2 cut(s)	433, 1820
PfoI	TCCNGGA	1 cut(s)	948
PkrI	GCNGC	4 cut(s)	1248, 2012, 2574, 2838
PleI	GAGTC	5 cut(s)	85, 230, 246, 609, 1096
PmaCI	CACGTG	1 cut(s)	2913
PmlI	CACGTG	1 cut(s)	2913
PpsI	GAGTC	5 cut(s)	85, 230, 246, 609, 1096
Ppu21I	YACGTR	3 cut(s)	1763, 2084, 2913
PscI	ACATGT	2 cut(s)	1958, 2620
PshAI	GACNNNNGTC	1 cut(s)	999
Psp1406I	AACGTT	1 cut(s)	1949
Psp6I	CCWGG	2 cut(s)	925, 948
PspCI	CACGTG	1 cut(s)	2913
PspEI	GGTNACC	2 cut(s)	281, 644
PspFI	CCCAGC	1 cut(s)	1598
PspGI	CCWGG	2 cut(s)	925, 948
PspN4I	GGNNCC	5 cut(s)	161, 262, 697, 1663, 2261
PspPI	GGNCC	4 cut(s)	160, 548, 696, 1661
PsuI	RGATCY	1 cut(s)	1538
PvuII	CAGCTG	1 cut(s)	1249
RruI	TCGCGA	1 cut(s)	2925
RsaI	GTAC	8 cut(s)	607, 625, 812, 959, 1127, 1618, 1911, 2587
RsaNI	GTAC	8 cut(s)	606, 624, 811, 958, 1126, 1617, 1910, 2586
RseI	CAYNNNNRTG	2 cut(s)	2441, 2786
Rsr2I	CGGWCCG	1 cut(s)	548
RsrII	CGGWCCG	1 cut(s)	548
SapI	GCTCTTC	2 cut(s)	2070, 2732
SatI	GCNGC	4 cut(s)	1247, 2011, 2573, 2837
Sau3AI	GATC	9 cut(s)	117, 552, 881, 1538, 2280, 2547, 2767, 2814, 2845
Sau96I	GGNCC	4 cut(s)	160, 548, 696, 1661
SchI	GAGTC	5 cut(s)	86, 231, 246, 609, 1097
ScrFI	CCNGG	4 cut(s)	694, 927, 950, 2734
SduI	GDGCHC	2 cut(s)	1484, 2496
SfaNI	GCATC	4 cut(s)	2219, 2476, 2683, 2784
SfcI	CTRYAG	1 cut(s)	2637
SinI	GGWCC	3 cut(s)	160, 548, 696
SmiMI	CAYNNNNRTG	2 cut(s)	2441, 2786
SmlI	CTYRAG	4 cut(s)	367, 1039, 1697, 2941
SmoI	CTYRAG	4 cut(s)	367, 1039, 1697, 2941
SphI	GCATGC	1 cut(s)	1879
SseBI	AGGCCT	3 cut(s)	113, 930, 1077
SsiI	CCGC	3 cut(s)	485, 2010, 2687
SspI	AATATT	3 cut(s)	784, 1458, 3014
StuI	AGGCCT	3 cut(s)	113, 930, 1077
StyD4I	CCNGG	4 cut(s)	692, 925, 948, 2732
StyI	CCWWGG	3 cut(s)	493, 1107, 1941
TaiI	ACGT	8 cut(s)	384, 1765, 1900, 1952, 1987, 2086, 2814, 2915
TaqI	TCGA	9 cut(s)	441, 563, 669, 880, 1141, 1718, 1771, 1918, 2681
TaqII	GACCGA	1 cut(s)	536
TatI	WGTACW	5 cut(s)	810, 957, 1125, 1616, 2585
TauI	GCSGC	1 cut(s)	2013
TfiI	GAWTC	8 cut(s)	477, 598, 772, 1138, 1594, 1810, 2266, 2828
TscAI	CASTG	5 cut(s)	625, 748, 2187, 2501, 2595
TseFI	GTSAC	6 cut(s)	67, 372, 644, 788, 992, 2604
TseI	GCWGC	3 cut(s)	1246, 2572, 2836
Tsp45I	GTSAC	6 cut(s)	67, 372, 644, 788, 992, 2604
TspDTI	ATGAA	9 cut(s)	567, 1000, 1074, 1340, 1371, 1627, 2213, 2552, 2766
TspGWI	ACGGA	2 cut(s)	1452, 2943
TspRI	CASTG	5 cut(s)	625, 748, 2187, 2501, 2595
Van91I	CCANNNNNTGG	2 cut(s)	433, 1820
Vha464I	CTTAAG	1 cut(s)	1039
VneI	GTGCAC	1 cut(s)	2492
VpaK11BI	GGWCC	3 cut(s)	160, 548, 696
XagI	CCTNNNNNAGG	1 cut(s)	1106
XapI	RAATTY	7 cut(s)	348, 523, 592, 846, 1143, 2098, 2113
XceI	RCATGY	4 cut(s)	895, 1879, 1962, 2624
XmiI	GTMKAC	1 cut(s)	2145
XmnI	GAANNNNTTC	2 cut(s)	1444, 1458
Zsp2I	ATGCAT	3 cut(s)	1877, 1881, 2698

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.