Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0005533

Physical Location & Seq

Genomic Coordinates Forward (+)

72432963 .. 72441709

Gene Structure 8747 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000035833

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3174 bp

ATGGATTCCCACTTCCATACTCTATTGCCTCCTCAGGTTTCCATGTTAGCTCTTCTTCTATGCATATCATTTCTGAGCGCAGAGTCTGCTATAATTCCAAGCATTACCACCGACAAGGAGGCCTTGCTCGATGTCCCTCCATCTTTAACCTGGGACCAGAATTCATCCCCTTGCAGGAATTGGACCGGAGTTGTTTGCAGAAAACATGGTCAGAGTTGGAGAGTTGTTGGTCTTGATCTTTCTGGCTTGGGACTTTCAGGGTCCATAAGCCCTCGCATTGGTAACTTATCCTCCCTCCAATCCCTTCAACTTCAAAACAACAAGTTAACAGGAGCAATTCCTACTCAAGTTGTTAACCTTTTCCGCATGAGATCTCTGAACCTGAGCTCCAACACCATACAAGGTCCACTACCTTCAAACATGAGCCACTTGACTGCACTAGAAATGCTCGACTTGGCAGGGAATAACATAACAGGCAGGCTACCTGAAGAGATATTCAGCCAAAGAAAACTTCGAGTCCTGAATCTGGGACGAAACAGTTTCTTTGGTTCAATTCCTTCATCCATAAGCAACCTCTCATCAACGCTCACATATTTGAATTTGGGTACAAATAGCCTCAGTGGGATCATACCTAGTGAGTTGGGTCTACTTATCAATCTCAAGGAACTTGATCTTTCTGGTAACAAGTTCACTGGGACTGTTGCTCCGTCCATATACAACATTACTTCATTGGTTCTGTTTACTGTTGCTTCAAATCAACTTTGGGGTGAGATACCCAAAAATATTGATGAAACACTTCCAAATCTTTTATACTGCCGGAACTGCTTTAATCTTATGACAGGGAAAATTCCAGCGTCTTTGCACAATGTCACCAAGATTCGATCAACTCGAATGTCAAACAATTTTCTGGAAGGAACAGTTCCACCTGGGCTGGGAAACATGCCATTCCTCGAAATGTACAACATTGGCTTCAATAGGATCATTAGTAAAGGCAGTGATGGTCTCAGTTTTATCACTTCCTTGACAAACAGCACCAACCTGCAATATCTTGCTATTAACGACAACCAGTTAGAGGGAGTCATTCCAGAATCACTTGGCAATCTCTCGAAGGTTCTCTCAAAATTGTACATGGGAGGGAATCGGATTTATGGTAACATTCCTGACTCAGTTGGTCATCTTAGTAGTTTAGCTTTGTTAAATGTGAGCTACAATTCAATATCCGGTGAAATTCCCACTGAAATAGGCCAATTGGAAAATCTACAAATGCTTGGTTTAGCAGGAAATCAACTGTCTGGTCATATTCCAAACTCTTTAGGAAATCTCAAAAAATTGAACGATCTTGATTTATCTGGGAATCACTTTAATCTGCTTTCCATGGACTTATCAAACAATCAGCTTAACGGAACCATTTCCGGGGAAACTCTCAACCTTCCTAGTTTGAGCACTATTCTCAATCTGTCCAAAAACTTTTTAATGTTCACTATAGACCTATCTGACAATGCATTATCTGGAGATATCCCTGGATCAATAGAAAAGTGTAAAAGCTTGGAGAGATTGTTGTTGTCAAGAAATAGAATTTCAGGTCTTATTCCCAATGGTCTAGGAGAATTGAGAGGCCTTGAGTTTCTAGACCTCTCCTCAAACCAGCTGTCCAGCTCCATTCCAGAGAATTTTCAAGACCTACATGCCCTTCGATACTTGAACCTATCATTTAATAACTTAGAAGGAGTGATTCCTAACGGTGGACTATTTGTTACAAATTTCACTAATGTTCATCTTGAAGGCAACCCAAAAATTTGCCTCAAATTTCCATGTGTGAAGAATATTGATGGTAGTCGAAGAAGAAAAATACTAGTCCCAGTTATGATCATTACAACAGTCTTCGCAACCATATGTGTTATAGTGGGCTGTTTGGTCTATGCTAAGAAAAGAAAAGGCTGCAGAGCAAAAATTACAGAAACTTGTGATGACTTAGAAGTCAAAGGGCAGCATCAAATGGTTTCGTATGAAGAACTCCGTCGCTCAACAGGGAATTTGAACCCGATAATTTGGTTGGAAGGAGATCAAGGAATTGAAGTTGCAGTCAAGGTGCTCGATATTAGAACAACAGGGTCTTGGAAGAGTTTCTTGGCAGAGTGCAATGCATTAAGGAGTGTAAGGCACCGAAATCTTGTCAAACCGATCACCTCCTGCTCAAGCTTAGACTCTAAGAACATGGAGTTTCTGGCTTTGGTTTATGAGTATCTGAGTAATGGGAGCTTGGAAGATTGGATTAAGGGCAAGAGAACAAATTTAGATGGAGACGGTTTGAGCATTGTGGAGAGATTGAATGCGGCAATTGATGTTGCTTGTGGATTGGATTACCTCCATAATGATTGTGAAGTTCCAGTTGGAGATTTTGGGCTGGCAAGGTTACTGATACAAGAAACATCCACTCAACATTCTATTGGCTCCACCAATGTCCTCAAGGGTTCCATAGGATATATACCCCCAGAATATGGTTTTGGACAAAAACCCTCCACAGCTGGAGATGCATACAGTTTTGGAGTAATGTTGTTGGAGCTGTTTACTGGAAAAAGTCCAACCGATGAAAGCTTCACAGGAGAAGTTAACCTCGTTCAATGGGTGCAGTCATCTTTCCCCCACAACATTGCACAAGTACTAGACTCTGAGTTGTTACACTATGAGCACGACGAAGACTCAAATCCCATCTCAGAAGAAGATCTGAATTGTCTGATCTCAGTTATCGAGGTTGGGCTCTCTTGCACCTTCGCCTCTCCTGATGGACGGATTAGCTTGAGGGATGCCCTTCACAAACTTGAAACTGCTAGAGAGACCCTTTTGAAGCTTGCTCACCTTGACAGTGCAGAGTGTAAATTTCGACAACAAATGCAGGTTGAGGAGGTGGTGGAGTTCTACAAATCATTTTCGGATGTAAACAGAGAAGCCTTTTGTTATGAGAGGCTCAAGCTCATCATCAATGATGCCAGGTATGTCTCGAACTTTATCCGGTCACCGCCCGGCGACAATGTTACAGTTCCAGGCTTCATGAAACAGTACCCGTGGTCAACCAGGACAATTCTGACGTCGTTTTTGCTGCCGTCTCAATGGCCCCAATGCCACATCAAAGAGCTCCTCCTCACCATGGAGGAGGCCTTACGACGAAAAGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009987 GO:0010204 GO:0010359 GO:0012501 GO:0012505 GO:0016020 GO:0016045 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031347 GO:0031349 GO:0032409 GO:0032412 GO:0032879 GO:0033554 GO:0034050 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080134 GO:0098543 GO:0098581 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

1058

Amino Acids

116.4

Weight (kDa)

5.87

Isoelectric Point (pI)

38.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	90 - 225	3.9e-11	Leucine-rich repeat region
LRR_8	PF13855	98 - 157	6.2e-09	Leucine rich repeat
LRR_8	PF13855	169 - 230	2.6e-06	Leucine rich repeat
LRR_14	PF23598	338 - 451	2e-10	Leucine-rich repeat region
LRR_8	PF13855	372 - 430	5.6e-07	Leucine rich repeat
LRR_8	PF13855	494 - 550	4.8e-09	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	686 - 865	3.8e-20	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	688 - 871	3.2e-15	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g36850 FvH4_3g36850 FvH4_3g36850
malus_domestica	MD08G1090900.v1.1 MD15G1074900.v1.1 MD15G1075000.v1.1
prunus_persica	Prupe.1G427400_v2.0.a1 Prupe.1G427700_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1
pyrus_communis	pycom15g06980 pycom15g06990
rosa_chinensis	RchiOBHm_Chr5g0066001 RchiOBHm_Chr5g0066021
rosa_laevigata	RLG00000035833 RLG00000035834
rosa_multiflora	Rmu_sc0001473.1_g000006 Rmu_sc0011576.1_g000012 Rmu_sc0011576.1_g000015
rosa_roxburghii	Rroxscaffold_1G00014980 Rroxscaffold_1G00015120
rosa_rugosa	Rorug05G0375800 Rorug05G0375900 Rorug05G0376000
rosa_samantha	Rh5AG434300 Rh5AG434500 Rh5CG472900
rosa_wichuraiana	Rw5G040690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1976
AatII	GACGTC	1 cut(s)	3089
Acc36I	ACCTGC	2 cut(s)	1047, 2884
AccB1I	GGYRCC	1 cut(s)	2160
AccB7I	CCANNNNNTGG	1 cut(s)	2498
AccI	GTMKAC	1 cut(s)	646
AciI	CCGC	3 cut(s)	364, 2333, 3017
AclWI	GGATC	3 cut(s)	632, 986, 1531
AcuI	CTGAAG	1 cut(s)	507
AcyI	GRCGYC	1 cut(s)	3086
AfaI	GTAC	5 cut(s)	607, 959, 1127, 2661, 3059
AfiI	CCNNNNNNNGG	9 cut(s)	174, 278, 526, 932, 1072, 1413, 1742, 2498, 3145
AhlI	ACTAGT	1 cut(s)	1852
AjnI	CCWGG	6 cut(s)	149, 925, 1519, 2987, 3040, 3071
AjuI	GAANNNNNNNTTGG	8 cut(s)	495, 527, 2111, 2143, 2373, 2405, 2487, 2519
AloI	GAACNNNNNNTCC	2 cut(s)	371, 403
Alw21I	GWGCWC	5 cut(s)	389, 1445, 2094, 2691, 3135
Alw26I	GTCTC	5 cut(s)	1007, 2295, 2828, 3001, 3108
AlwI	GGATC	3 cut(s)	632, 986, 1531
AlwNI	CAGNNNCTG	1 cut(s)	86
AoxI	GGCC	5 cut(s)	120, 1243, 1615, 3110, 3153
ApeKI	GCWGC	3 cut(s)	1938, 1987, 3097
ArsI	GACNNNNNNTTYG	4 cut(s)	1634, 1666, 3076, 3108
Asp700I	GAANNNNTTC	3 cut(s)	795, 1408, 2123
AspLEI	GCGC	1 cut(s)	80
AspS9I	GGNCC	5 cut(s)	154, 183, 261, 404, 3111
AsuC2I	CCSGG	2 cut(s)	1414, 3021
AsuHPI	GGTGA	7 cut(s)	779, 862, 1235, 2176, 2846, 3006, 3133
AvaII	GGWCC	4 cut(s)	154, 183, 261, 404
AxyI	CCTNAGG	1 cut(s)	33
BanI	GGYRCC	1 cut(s)	2160
BanII	GRGCYC	3 cut(s)	389, 2760, 3135
BbsI	GAAGAC	2 cut(s)	1873, 2703
Bbv12I	GWGCWC	5 cut(s)	389, 1445, 2094, 2691, 3135
BbvI	GCAGC	3 cut(s)	1925, 1999, 3084
BccI	CCATC	6 cut(s)	148, 992, 1823, 2291, 2717, 2777
BceAI	ACGGC	1 cut(s)	3085
BciT130I	CCWGG	6 cut(s)	151, 927, 1521, 2989, 3042, 3073
BclI	TGATCA	1 cut(s)	1866
BcnI	CCSGG	2 cut(s)	1414, 3021
BcoDI	GTCTC	5 cut(s)	1007, 2295, 2828, 3001, 3108
BcuI	ACTAGT	1 cut(s)	1852
BfaI	CTAG	8 cut(s)	440, 633, 1434, 1601, 1628, 1853, 2663, 2829
BfmI	CTRYAG	2 cut(s)	1482, 1939
BfuAI	ACCTGC	2 cut(s)	1047, 2884
BglII	AGATCT	2 cut(s)	371, 2722
BisI	GCNGC	4 cut(s)	1939, 1988, 2334, 3098
BlsI	GCNGC	4 cut(s)	1940, 1989, 2335, 3099
BmcAI	AGTACT	1 cut(s)	2661
Bme1390I	CCNGG	8 cut(s)	151, 927, 1414, 1521, 2989, 3021, 3042, 3073
Bme18I	GGWCC	4 cut(s)	154, 183, 261, 404
BmgT120I	GGNCC	5 cut(s)	154, 183, 261, 404, 3111
BmiI	GGNNCC	7 cut(s)	155, 262, 1405, 2162, 2452, 2473, 3113
BmrFI	CCNGG	8 cut(s)	151, 927, 1414, 1521, 2989, 3021, 3042, 3073
BmrI	ACTGGG	2 cut(s)	702, 1853
BmsI	GCATC	4 cut(s)	1999, 2521, 2794, 2974
BmuI	ACTGGG	2 cut(s)	702, 1853
BpiI	GAAGAC	2 cut(s)	1873, 2703
BpmI	CTGGAG	2 cut(s)	1530, 2547
Bpu10I	CCTNAGC	1 cut(s)	383
BpuEI	CTTGAG	7 cut(s)	330, 644, 1640, 2179, 2450, 2818, 2951
BpuMI	CCSGG	2 cut(s)	1414, 3021
BsaHI	GRCGYC	1 cut(s)	3086
BsaI	GGTCTC	2 cut(s)	1007, 2828
BsaJI	CCNNGG	7 cut(s)	150, 926, 1374, 1413, 1519, 3062, 3144
BsaWI	WCCGGW	3 cut(s)	185, 1220, 3009
BsaXI	ACNNNNNCTCC	8 cut(s)	275, 305, 371, 401, 688, 718, 1541, 1571
Bsc4I	CCNNNNNNNGG	9 cut(s)	174, 278, 526, 932, 1072, 1413, 1742, 2498, 3145
Bse1I	ACTGG	5 cut(s)	697, 1066, 1859, 2387, 2575
Bse21I	CCTNAGG	1 cut(s)	33
Bse3DI	GCAATG	2 cut(s)	2146, 2649
BseBI	CCWGG	6 cut(s)	151, 927, 1521, 2989, 3042, 3073
BseDI	CCNNGG	7 cut(s)	150, 926, 1374, 1413, 1519, 3062, 3144
BseGI	GGATG	5 cut(s)	164, 560, 2429, 2809, 2938
BseLI	CCNNNNNNNGG	9 cut(s)	174, 278, 526, 932, 1072, 1413, 1742, 2498, 3145
BseMI	GCAATG	2 cut(s)	2146, 2649
BseNI	ACTGG	5 cut(s)	697, 1066, 1859, 2387, 2575
BseRI	GAGGAG	6 cut(s)	21, 1627, 2915, 3125, 3128, 3164
BseXI	GCAGC	3 cut(s)	1925, 1999, 3084
BseYI	CCCAGC	1 cut(s)	931
BsgI	GTGCAG	3 cut(s)	420, 2648, 2886
BshFI	GGCC	5 cut(s)	122, 1245, 1617, 3112, 3155
BshNI	GGYRCC	1 cut(s)	2160
BsiHKAI	GWGCWC	5 cut(s)	389, 1445, 2094, 2691, 3135
BsiSI	CCGG	6 cut(s)	186, 817, 1221, 1413, 3010, 3021
BslFI	GGGAC	6 cut(s)	119, 167, 264, 543, 709, 1841
BslI	CCNNNNNNNGG	9 cut(s)	174, 278, 526, 932, 1072, 1413, 1742, 2498, 3145
BsmAI	GTCTC	5 cut(s)	1007, 2295, 2828, 3001, 3108
BsmBI	CGTCTC	2 cut(s)	2295, 3108
BsmFI	GGGAC	6 cut(s)	119, 167, 264, 543, 709, 1841
BsmI	GAATGC	1 cut(s)	2335
BsnI	GGCC	5 cut(s)	122, 1245, 1617, 3112, 3155
Bso31I	GGTCTC	2 cut(s)	1007, 2828
Bsp1286I	GDGCHC	6 cut(s)	389, 1445, 2094, 2691, 2760, 3135
Bsp1407I	TGTACA	2 cut(s)	957, 1125
Bsp19I	CCATGG	2 cut(s)	1374, 3144
BspACI	CCGC	3 cut(s)	364, 2333, 3017
BspANI	GGCC	5 cut(s)	122, 1245, 1617, 3112, 3155
BspHI	TCATGA	1 cut(s)	3048
BspLI	GGNNCC	7 cut(s)	155, 262, 1405, 2162, 2452, 2473, 3113
BspMAI	CTGCAG	1 cut(s)	1943
BspMI	ACCTGC	2 cut(s)	1047, 2884
BspPI	GGATC	3 cut(s)	632, 986, 1531
BspQI	GCTCTTC	1 cut(s)	57
BspT107I	GGYRCC	1 cut(s)	2160
BspTNI	GGTCTC	2 cut(s)	1007, 2828
BsrDI	GCAATG	2 cut(s)	2146, 2649
BsrGI	TGTACA	2 cut(s)	957, 1125
BsrI	ACTGG	5 cut(s)	697, 1066, 1859, 2387, 2575
BssECI	CCNNGG	7 cut(s)	150, 926, 1374, 1413, 1519, 3062, 3144
BssNI	GRCGYC	1 cut(s)	3086
BssT1I	CCWWGG	2 cut(s)	1374, 3144
Bst2UI	CCWGG	6 cut(s)	151, 927, 1521, 2989, 3042, 3073
Bst6I	CTCTTC	3 cut(s)	57, 483, 2114
BstACI	GRCGYC	1 cut(s)	3086
BstAPI	GCANNNNNTGC	1 cut(s)	86
BstAUI	TGTACA	2 cut(s)	957, 1125
BstC8I	GCNNGC	3 cut(s)	479, 2406, 2850
BstDSI	CCRYGG	3 cut(s)	1374, 3062, 3144
BstEII	GGTNACC	1 cut(s)	3012
BstF5I	GGATG	5 cut(s)	164, 560, 2429, 2809, 2938
BstHHI	GCGC	1 cut(s)	80
BstMAI	GTCTC	5 cut(s)	1007, 2295, 2828, 3001, 3108
BstMWI	GCNNNNNNNGC	3 cut(s)	86, 822, 2531
BstNI	CCWGG	6 cut(s)	151, 927, 1521, 2989, 3042, 3073
BstNSI	RCATGY	2 cut(s)	943, 1688
BstPI	GGTNACC	1 cut(s)	3012
BstSCI	CCNGG	8 cut(s)	149, 925, 1412, 1519, 2987, 3019, 3040, 3071
BstSFI	CTRYAG	2 cut(s)	1482, 1939
BstV1I	GCAGC	3 cut(s)	1925, 1999, 3084
BstV2I	GAAGAC	2 cut(s)	1873, 2703
BstX2I	RGATCY	2 cut(s)	371, 2722
BstYI	RGATCY	2 cut(s)	371, 2722
Bsu36I	CCTNAGG	1 cut(s)	33
BsuRI	GGCC	5 cut(s)	122, 1245, 1617, 3112, 3155
BtgI	CCRYGG	3 cut(s)	1374, 3062, 3144
BtsCI	GGATG	5 cut(s)	164, 560, 2429, 2809, 2938
BtsI	GCAGTG	1 cut(s)	1000
BtsIMutI	CAGTG	5 cut(s)	625, 690, 1000, 1233, 2869
BveI	ACCTGC	2 cut(s)	1047, 2884
Cac8I	GCNNGC	3 cut(s)	479, 2406, 2850
CaiI	CAGNNNCTG	1 cut(s)	86
CciI	TCATGA	1 cut(s)	3048
CfoI	GCGC	1 cut(s)	80
Cfr13I	GGNCC	5 cut(s)	154, 183, 261, 404, 3111
CseI	GACGC	1 cut(s)	843
Csp6I	GTAC	5 cut(s)	606, 958, 1126, 2660, 3058
CviQI	GTAC	5 cut(s)	606, 958, 1126, 2660, 3058
DrdI	GACNNNNNNGTC	1 cut(s)	1976
DseDI	GACNNNNNNGTC	1 cut(s)	1976
Eam1104I	CTCTTC	3 cut(s)	57, 483, 2114
EarI	CTCTTC	3 cut(s)	57, 483, 2114
Ecl136II	GAGCTC	2 cut(s)	387, 3133
Eco130I	CCWWGG	2 cut(s)	1374, 3144
Eco147I	AGGCCT	3 cut(s)	122, 1617, 3155
Eco24I	GRGCYC	3 cut(s)	389, 2760, 3135
Eco31I	GGTCTC	2 cut(s)	1007, 2828
Eco32I	GATATC	1 cut(s)	1516
Eco47I	GGWCC	4 cut(s)	154, 183, 261, 404
Eco53kI	GAGCTC	2 cut(s)	387, 3133
Eco57I	CTGAAG	1 cut(s)	507
Eco81I	CCTNAGG	1 cut(s)	33
Eco91I	GGTNACC	1 cut(s)	3012
EcoICRI	GAGCTC	2 cut(s)	387, 3133
EcoO65I	GGTNACC	1 cut(s)	3012
EcoRI	GAATTC	1 cut(s)	160
EcoRII	CCWGG	6 cut(s)	149, 925, 1519, 2987, 3040, 3071
EcoRV	GATATC	1 cut(s)	1516
EcoT14I	CCWWGG	2 cut(s)	1374, 3144
EcoT22I	ATGCAT	4 cut(s)	65, 1504, 2146, 2536
EcoT38I	GRGCYC	3 cut(s)	389, 2760, 3135
ErhI	CCWWGG	2 cut(s)	1374, 3144
Esp3I	CGTCTC	2 cut(s)	2295, 3108
FalI	AAGNNNNNCTT	4 cut(s)	107, 139, 2111, 2143
FaqI	GGGAC	6 cut(s)	119, 167, 264, 543, 709, 1841
FauNDI	CATATG	1 cut(s)	1892
FbaI	TGATCA	1 cut(s)	1866
FblI	GTMKAC	1 cut(s)	646
Fnu4HI	GCNGC	4 cut(s)	1939, 1988, 2334, 3098
FokI	GGATG	5 cut(s)	151, 547, 2416, 2816, 2945
FriOI	GRGCYC	3 cut(s)	389, 2760, 3135
Fsp4HI	GCNGC	4 cut(s)	1939, 1988, 2334, 3098
FspBI	CTAG	8 cut(s)	440, 633, 1434, 1601, 1628, 1853, 2663, 2829
GlaI	GCGC	1 cut(s)	79
GluI	GCNGC	4 cut(s)	1939, 1988, 2334, 3098
GsaI	CCCAGC	1 cut(s)	935
GsuI	CTGGAG	2 cut(s)	1530, 2547
HaeIII	GGCC	5 cut(s)	122, 1245, 1617, 3112, 3155
HapII	CCGG	6 cut(s)	186, 817, 1221, 1413, 3010, 3021
HgaI	GACGC	1 cut(s)	843
HhaI	GCGC	1 cut(s)	80
Hin1I	GRCGYC	1 cut(s)	3086
Hin6I	GCGC	1 cut(s)	78
HinP1I	GCGC	1 cut(s)	78
HincII	GTYRAC	4 cut(s)	327, 355, 2611, 3069
HindII	GTYRAC	4 cut(s)	327, 355, 2611, 3069
HindIII	AAGCTT	4 cut(s)	1543, 2197, 2593, 2846
HpaI	GTTAAC	3 cut(s)	327, 355, 2611
HpaII	CCGG	6 cut(s)	186, 817, 1221, 1413, 3010, 3021
HphI	GGTGA	7 cut(s)	779, 862, 1235, 2176, 2846, 3006, 3133
Hpy99I	CGWCG	4 cut(s)	2022, 2696, 3091, 3165
HpyCH4IV	ACGT	1 cut(s)	3086
HpyF10VI	GCNNNNNNNGC	3 cut(s)	86, 822, 2531
HpySE526I	ACGT	1 cut(s)	3086
Hsp92I	GRCGYC	1 cut(s)	3086
HspAI	GCGC	1 cut(s)	78
Ksp22I	TGATCA	1 cut(s)	1866
KspAI	GTTAAC	3 cut(s)	327, 355, 2611
LguI	GCTCTTC	1 cut(s)	57
LmnI	GCTCC	8 cut(s)	332, 392, 709, 1661, 2256, 2456, 2560, 3138
Lsp1109I	GCAGC	3 cut(s)	1925, 1999, 3084
LweI	GCATC	4 cut(s)	1999, 2521, 2794, 2974
MaeI	CTAG	8 cut(s)	440, 633, 1434, 1601, 1628, 1853, 2663, 2829
MaeII	ACGT	1 cut(s)	3086
MaeIII	GTNAC	9 cut(s)	281, 680, 868, 1151, 1753, 2412, 2676, 3012, 3031
MfeI	CAATTG	2 cut(s)	1247, 2337
MflI	RGATCY	2 cut(s)	371, 2722
MhlI	GDGCHC	6 cut(s)	389, 1445, 2094, 2691, 2760, 3135
MlyI	GAGTC	7 cut(s)	92, 525, 1086, 1157, 2198, 2660, 2693
MmeI	TCCRAC	6 cut(s)	197, 414, 2034, 2371, 2538, 2606
Mph1103I	ATGCAT	4 cut(s)	65, 1504, 2146, 2536
MroXI	GAANNNNTTC	3 cut(s)	795, 1408, 2123
MspA1I	CMGCKG	2 cut(s)	1648, 2525
MspI	CCGG	6 cut(s)	186, 817, 1221, 1413, 3010, 3021
MspR9I	CCNGG	8 cut(s)	151, 927, 1414, 1521, 2989, 3021, 3042, 3073
MunI	CAATTG	2 cut(s)	1247, 2337
Mva1269I	GAATGC	1 cut(s)	2335
MvaI	CCWGG	6 cut(s)	151, 927, 1521, 2989, 3042, 3073
MwoI	GCNNNNNNNGC	3 cut(s)	86, 822, 2531
NciI	CCSGG	2 cut(s)	1414, 3021
NcoI	CCATGG	2 cut(s)	1374, 3144
NdeI	CATATG	1 cut(s)	1892
NlaIV	GGNNCC	7 cut(s)	155, 262, 1405, 2162, 2452, 2473, 3113
NmuCI	GTSAC	2 cut(s)	868, 3012
NsiI	ATGCAT	4 cut(s)	65, 1504, 2146, 2536
NspI	RCATGY	2 cut(s)	943, 1688
PagI	TCATGA	1 cut(s)	3048
PceI	AGGCCT	3 cut(s)	122, 1617, 3155
PciSI	GCTCTTC	1 cut(s)	57
PcsI	WCGNNNNNNNCGW	1 cut(s)	886
PctI	GAATGC	1 cut(s)	2335
PdmI	GAANNNNTTC	3 cut(s)	795, 1408, 2123
PfeI	GAWTC	7 cut(s)	5, 523, 877, 1088, 1138, 1354, 1732
PflMI	CCANNNNNTGG	1 cut(s)	2498
PkrI	GCNGC	4 cut(s)	1940, 1989, 2335, 3099
PleI	GAGTC	7 cut(s)	91, 524, 1085, 1157, 2198, 2660, 2693
PpsI	GAGTC	7 cut(s)	91, 524, 1085, 1157, 2198, 2660, 2693
Psp124BI	GAGCTC	2 cut(s)	389, 3135
Psp6I	CCWGG	6 cut(s)	149, 925, 1519, 2987, 3040, 3071
PspEI	GGTNACC	1 cut(s)	3012
PspFI	CCCAGC	1 cut(s)	931
PspGI	CCWGG	6 cut(s)	149, 925, 1519, 2987, 3040, 3071
PspN4I	GGNNCC	7 cut(s)	155, 262, 1405, 2162, 2452, 2473, 3113
PspPI	GGNCC	5 cut(s)	154, 183, 261, 404, 3111
PstI	CTGCAG	1 cut(s)	1943
PstNI	CAGNNNCTG	1 cut(s)	86
PsuI	RGATCY	2 cut(s)	371, 2722
PvuII	CAGCTG	2 cut(s)	1648, 2525
RsaI	GTAC	5 cut(s)	607, 959, 1127, 2661, 3059
RsaNI	GTAC	5 cut(s)	606, 958, 1126, 2660, 3058
SacI	GAGCTC	2 cut(s)	389, 3135
SapI	GCTCTTC	1 cut(s)	57
SatI	GCNGC	4 cut(s)	1939, 1988, 2334, 3098
Sau96I	GGNCC	5 cut(s)	154, 183, 261, 404, 3111
ScaI	AGTACT	1 cut(s)	2661
SchI	GAGTC	7 cut(s)	92, 525, 1086, 1157, 2198, 2660, 2693
ScrFI	CCNGG	8 cut(s)	151, 927, 1414, 1521, 2989, 3021, 3042, 3073
SduI	GDGCHC	6 cut(s)	389, 1445, 2094, 2691, 2760, 3135
SfaNI	GCATC	4 cut(s)	1999, 2521, 2794, 2974
SfcI	CTRYAG	2 cut(s)	1482, 1939
SinI	GGWCC	4 cut(s)	154, 183, 261, 404
SmlI	CTYRAG	7 cut(s)	345, 659, 1619, 2194, 2465, 2797, 2966
SmoI	CTYRAG	7 cut(s)	345, 659, 1619, 2194, 2465, 2797, 2966
SpeI	ACTAGT	1 cut(s)	1852
SseBI	AGGCCT	3 cut(s)	122, 1617, 3155
SsiI	CCGC	3 cut(s)	364, 2333, 3017
SspI	AATATT	2 cut(s)	784, 1825
SspMI	CTAG	8 cut(s)	440, 633, 1434, 1601, 1628, 1853, 2663, 2829
SstI	GAGCTC	2 cut(s)	389, 3135
StuI	AGGCCT	3 cut(s)	122, 1617, 3155
StyD4I	CCNGG	8 cut(s)	149, 925, 1412, 1519, 2987, 3019, 3040, 3071
StyI	CCWWGG	2 cut(s)	1374, 3144
TaiI	ACGT	1 cut(s)	3089
TatI	WGTACW	3 cut(s)	957, 1125, 2659
TauI	GCSGC	1 cut(s)	2336
TfiI	GAWTC	7 cut(s)	5, 523, 877, 1088, 1138, 1354, 1732
TscAI	CASTG	5 cut(s)	625, 697, 1000, 1240, 2869
TseFI	GTSAC	2 cut(s)	868, 3012
TseI	GCWGC	3 cut(s)	1938, 1987, 3097
Tsp45I	GTSAC	2 cut(s)	868, 3012
TspDTI	ATGAA	9 cut(s)	153, 549, 717, 804, 1763, 2022, 2604, 3037, 3065
TspGWI	ACGGA	4 cut(s)	696, 1416, 2006, 2803
TspRI	CASTG	5 cut(s)	625, 697, 1000, 1240, 2869
Van91I	CCANNNNNTGG	1 cut(s)	2498
VpaK11BI	GGWCC	4 cut(s)	154, 183, 261, 404
XbaI	TCTAGA	1 cut(s)	1627
XceI	RCATGY	2 cut(s)	943, 1688
XcmI	CCANNNNNNNNNTGG	1 cut(s)	147
XmiI	GTMKAC	1 cut(s)	646
XmnI	GAANNNNTTC	3 cut(s)	795, 1408, 2123
XspI	CTAG	8 cut(s)	440, 633, 1434, 1601, 1628, 1853, 2663, 2829
ZraI	GACGTC	1 cut(s)	3087
ZrmI	AGTACT	1 cut(s)	2661
Zsp2I	ATGCAT	4 cut(s)	65, 1504, 2146, 2536

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.