Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0005533

Physical Location & Seq

Genomic Coordinates Forward (+)

5115101 .. 5118381

Gene Structure 3281 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1075000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2847 bp

ATGAATTCTCACATTGCCGTAACATTATTATCTGTTCTTGCATGCATTTTCTTGAGTGTAGACTCAGCATTCACTCCAAGCAATGTCACAGACAAGGAGGCCTTGATATCATTCCAATCTACGGCCAATCTCCCTCCATTTTTGTGGAACCAAAGCTCATCCCCCTGCACCAACTGGACCGGAGTTGCTTGCAACAGAATCGGTGGGACTCAACGGGTTGTTTCTCTCGATCTTTCCGGCTTCGGACTCACTGGCTCCATAAGCCACCATGTTGGGAGCTTGACAGAGCTTCAAGTCTTGAACTTGGCGCGCAACAATCTCAGCGGTCCAATCCCGCCATCCATAAGCAACCTGTCTTCCACCCTCACAAGTTTGAATCTTGGCACGAACAGCCTCACCGGTATAATCCCAATGGAATTAGGTTTTCTTCGTAAGCTCAAAGAACTTGATCTTGCCGGCAACCAACTCACCGGCACTGTTGCCTTGTCCATATACAACATCTCTTCGCTAGTTCGCTTCACCGTTGCTTCAAACCAACTCTGGGGTGAAATTCCAAGCAACATTGGCGTCACGCTGCCAAACCTTCTCTACTTCAGAAATTGTTTCAATCTGTTTACAGGGAAAATTCCAGCGTCCTTGCACAACATTACAAGGATTCGAGAGCTCCGACTGTCCAACAATTTCCATGACGGTTCAGTTCCACCAGGCCTCGGAAATCTTCCCTTCCTTGAAATGTACAACATTGGTTTCAATCGGATCGTTAGCTATGGTGACAATGGTCTTGATTTCATTACTTCTTTGACAAACAGCAGTCGCTTAAGTTGGCTTGCAATTGACGAAAATCGGTTGGAAGGCCTAATTCCAGAGTCCATTGGCAACCTCTCCAAGGTTCTTTCTAAATTGTACATGGGAGGGAATCGAATTTATGGTAACATACCTGCCTCAATTAGTCAACTCAGTAGCTTAACTTTGCTAAATGTGAGCTACAATTCAATTTCTGGTGAAATCCCATCAGAAATTGGGCAGCTGAAAGAGTTGCAAGTGCTTGGGTTAGCAAGAAACAAATTGTCTGGCACAATTCCGAACTCCTTAGGTAATATGATGAAACTGAACCGCATTGATTTATCTGGAAATTACTTTGTTGGCCATATACCCCTTTCATTTTCAAACTTTCAGAAATTGCTTTCCATGGACTTATCTGACAATTCGCTCAACGGAAGCATTTCCGGAGAAATATTTCTAAACCTTCCTAGTTTGAGCACAATTTTGAATTTGTCTAACAACCTTCTAAGTGGACCTTTGCCTGAAGAAATTGGAGTACTAGCAAATGTTGTCACTATAGACCTCTCCAACAATGGCTTATCGGGGTCAATTCCCAGCTCAATTGGAAAATGTACAAGCTTGGAGAGATTGTTCATTGCAAGAAACAACCTCTCCGGTCCTCTTCCAAATGCTCTTGGAGAAATCAGAGGTCTTGAGATTTTAGACCTCTCCTCGAACAAGCTTTCTGGTTTTATTCCTGATAAACTTCAAGAACTACAGGTCCTTAGCTTCTTGAACCTCTCATTTAACAGCTTAGAGGGAGTAATTCCTAATGGTGGAGTGTTTGTTAGAAATATCTCAAGTGTATATCTAGAAGGAAACTGGAAACTTTGCCGAGATTGGCCATGTGCGAAGAGTGGTGGTCAGAGGAGAAAAGTACTGGTTTTAGTTCTTATCATAACATCTGTTCTGGCAGCATTGGTAGGCATTGTGGTAATTGGCTGGTTGCTTTGTGTTAGGAAAAGAAAAGGCTTAGGCTGCAAAACAACAATTAGAGAAACTTCTCCTGATTTATTAATAAAAGGGCAACATCAATTGGTCACATACGAAGAGCTCCGCAGCGCAACTGGGAATTTCAACCCGGAAAATTTGGTTGGAAGTGGGAGCTTTGGGACTGTATACAGAGGGTTTTTAAGACAAGGGACCGAAGTTGCAGTGAAGGTGCTTGATATAAAAATGACAGGATCTTGGAAGAGTTTTTTGGCAGAATGTGAGGCGTTGAGGAACGTGAGGCACCGCAATCTTGTTAAGTTGATCACTTCGTGCTCGAGGGTGGATTTTAAAAACATGGAGTTTCTGGCCTTGATTTATGAGTATTTGAGTAATGGGAGCTTGGAAGATTGGATTAAGGGCAAGAAAAAGAAAGCAGATGGAGATGGATTGAACATTTTGGAGAGACTCAACGTGGCCATTGATGTGGCGAGTGGATTAGATTATCTGCACCACGATTGCGAAGTTCCAGTTGTACATTGTGATTTAAAGCCGAGCAACATTCTTTTGGATGGAGACATGACTGCAAAGATTGGAGATTTTGGGCTGGCGAAGCTGCTCATGGAGAGAACAGAGATTACTCAACATTCTCTTAGCTCCACAAATGTTCTAAAGGGTTCTATAGGATACATACCACCAGAGTATGGTTTTGGACAGAAACCATCGACAGCGGGAGATGCATACAGTTTTGGAGTGATGTTGTTGGAGCTCTTCACTGGAAAAAGTCCAACCGACGAAAGTTTCACAGGAGACGAAAACCTCATCAGATGGGTGCAATCAGCCATCCCTCACAACATGATCAAAGTGCTAGACCCCGAGCTGCTAGACCTCATGCACTGCCTGCCTAATGAAGGGCCGTATCTCATCCCAGCAGAAGAACATAATTGCCTGGTCTTAATTTTCGAAGTTGGACTCTCTTGCACTTGCGTTTCGCCTGACGGACGCAGTAGCTTGAGGGAGGCACTTTACAAACTAGAAACTGCTAGGCAAACTCTGTTCAAAGTTGAGAGTGTCAACTGTCAAGCGTTAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009987 GO:0010204 GO:0010359 GO:0012501 GO:0012505 GO:0016020 GO:0016045 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031347 GO:0031349 GO:0032409 GO:0032412 GO:0032879 GO:0033554 GO:0034050 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080134 GO:0098543 GO:0098581 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

949

Amino Acids

103.15

Weight (kDa)

6.35

Isoelectric Point (pI)

33.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	96 - 156	5.6e-07	Leucine rich repeat
LRR_14	PF23598	267 - 375	1.6e-08	Leucine-rich repeat region
LRR_14	PF23598	386 - 525	3.8e-10	Leucine-rich repeat region
LRR_8	PF13855	443 - 502	1.8e-06	Leucine rich repeat
LRR_8	PF13855	467 - 526	2.1e-06	Leucine rich repeat
Pkinase	PF00069	635 - 883	6.7e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	637 - 883	1.9e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g36850 FvH4_3g36850 FvH4_3g36850
malus_domestica	MD08G1090900.v1.1 MD15G1074900.v1.1 MD15G1075000.v1.1
prunus_persica	Prupe.1G427400_v2.0.a1 Prupe.1G427700_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1
pyrus_communis	pycom15g06980 pycom15g06990
rosa_chinensis	RchiOBHm_Chr5g0066001 RchiOBHm_Chr5g0066021
rosa_laevigata	RLG00000035833 RLG00000035834
rosa_multiflora	Rmu_sc0001473.1_g000006 Rmu_sc0011576.1_g000012 Rmu_sc0011576.1_g000015
rosa_roxburghii	Rroxscaffold_1G00014980 Rroxscaffold_1G00015120
rosa_rugosa	Rorug05G0375800 Rorug05G0375900 Rorug05G0376000
rosa_samantha	Rh5AG434300 Rh5AG434500 Rh5CG472900
rosa_wichuraiana	Rw5G040690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	946
AccB1I	GGYRCC	1 cut(s)	2055
AccB7I	CCANNNNNTGG	1 cut(s)	2456
AccI	GTMKAC	2 cut(s)	60, 1941
AccII	CGCG	1 cut(s)	310
AccIII	TCCGGA	1 cut(s)	1226
AciI	CCGC	6 cut(s)	324, 335, 1114, 1879, 2059, 2483
AclWI	GGATC	2 cut(s)	764, 2014
AcoI	YGGCCR	4 cut(s)	123, 1144, 1664, 2229
AcsI	RAATTY	7 cut(s)	4, 549, 624, 921, 1270, 1894, 1909
AcuI	CTGAAG	2 cut(s)	577, 1326
AcyI	GRCGYC	1 cut(s)	567
AdeI	CACNNNGTG	1 cut(s)	2085
AfaI	GTAC	6 cut(s)	737, 905, 1320, 1396, 1701, 2289
AfiI	CCNNNNNNNGG	7 cut(s)	121, 541, 710, 886, 1598, 2456, 2663
AflII	CTTAAG	1 cut(s)	817
AgeI	ACCGGT	1 cut(s)	398
AjnI	CCWGG	2 cut(s)	703, 2700
AjuI	GAANNNNNNNTTGG	4 cut(s)	1899, 1931, 2006, 2038
AloI	GAACNNNNNNTCC	4 cut(s)	1397, 1429, 1527, 1559
Alw21I	GWGCWC	5 cut(s)	666, 1262, 1878, 2090, 2523
Alw26I	GTCTC	3 cut(s)	2212, 2322, 2556
AlwI	GGATC	2 cut(s)	764, 2014
Ama87I	CYCGRG	2 cut(s)	2089, 2627
Aor13HI	TCCGGA	1 cut(s)	1226
AoxI	GGCC	9 cut(s)	99, 123, 706, 853, 1144, 1664, 2121, 2229, 2666
ApeKI	GCWGC	7 cut(s)	574, 1024, 1736, 1800, 1881, 2368, 2632
ApoI	RAATTY	7 cut(s)	4, 549, 624, 921, 1270, 1894, 1909
AseI	ATTAAT	1 cut(s)	1838
AsiGI	ACCGGT	1 cut(s)	398
Asp700I	GAANNNNTTC	2 cut(s)	1222, 1236
AspLEI	GCGC	3 cut(s)	310, 312, 1886
AspS9I	GGNCC	7 cut(s)	177, 326, 1295, 1439, 1543, 1965, 2666
AsuC2I	CCSGG	1 cut(s)	1904
AsuHPI	GGTGA	6 cut(s)	388, 460, 511, 557, 782, 1013
AsuII	TTCGAA	1 cut(s)	2715
AvaI	CYCGRG	2 cut(s)	2089, 2627
AvaII	GGWCC	6 cut(s)	177, 326, 1295, 1439, 1543, 1965
AxyI	CCTNAGG	1 cut(s)	1090
BalI	TGGCCA	3 cut(s)	1146, 1666, 2231
BanI	GGYRCC	1 cut(s)	2055
BanII	GRGCYC	3 cut(s)	666, 1878, 2523
BbsI	GAAGAC	1 cut(s)	348
Bbv12I	GWGCWC	5 cut(s)	666, 1262, 1878, 2090, 2523
BbvI	GCAGC	7 cut(s)	561, 1036, 1748, 1787, 1893, 2355, 2619
BccI	CCATC	8 cut(s)	346, 1018, 2186, 2192, 2318, 2482, 2574, 2603
BceAI	ACGGC	3 cut(s)	2, 138, 2653
BcgI	CGANNNNNNTGC	2 cut(s)	181, 215
BciT130I	CCWGG	2 cut(s)	705, 2702
BciVI	GTATCC	1 cut(s)	2432
BclI	TGATCA	2 cut(s)	2076, 2610
BcnI	CCSGG	1 cut(s)	1904
BcoDI	GTCTC	3 cut(s)	2212, 2322, 2556
BfaI	CTAG	8 cut(s)	509, 1251, 1322, 1634, 2621, 2636, 2786, 2796
BfmI	CTRYAG	3 cut(s)	1338, 1538, 2433
BfrI	CTTAAG	1 cut(s)	817
BfuAI	ACCTGC	1 cut(s)	946
BfuI	GTATCC	1 cut(s)	2432
BisI	GCNGC	7 cut(s)	575, 1025, 1737, 1801, 1882, 2369, 2633
BlsI	GCNGC	7 cut(s)	576, 1026, 1738, 1802, 1883, 2370, 2634
BmcAI	AGTACT	2 cut(s)	1320, 1701
Bme1390I	CCNGG	3 cut(s)	705, 1904, 2702
Bme18I	GGWCC	6 cut(s)	177, 326, 1295, 1439, 1543, 1965
BmeT110I	CYCGRG	2 cut(s)	2089, 2627
BmgT120I	GGNCC	7 cut(s)	177, 326, 1295, 1439, 1543, 1965, 2666
BmiI	GGNNCC	4 cut(s)	149, 256, 1966, 2057
BmrFI	CCNGG	3 cut(s)	705, 1904, 2702
BmrI	ACTGGG	1 cut(s)	1899
BmsI	GCATC	1 cut(s)	2479
BmuI	ACTGGG	1 cut(s)	1899
BoxI	GACNNNNGTC	1 cut(s)	777
BpiI	GAAGAC	1 cut(s)	348
Bpu10I	CCTNAGC	2 cut(s)	1547, 1795
Bpu14I	TTCGAA	1 cut(s)	2715
BpuEI	CTTGAG	4 cut(s)	73, 1496, 1606, 2785
BpuMI	CCSGG	1 cut(s)	1904
BsaHI	GRCGYC	1 cut(s)	567
BsaJI	CCNNGG	3 cut(s)	709, 885, 1188
BsaWI	WCCGGW	4 cut(s)	179, 398, 1226, 1436
BsaXI	ACNNNNNCTCC	6 cut(s)	1397, 1418, 1427, 1448, 1593, 1623
Bsc4I	CCNNNNNNNGG	7 cut(s)	121, 541, 710, 886, 1598, 2456, 2663
Bse118I	RCCGGY	3 cut(s)	398, 455, 470
Bse1I	ACTGG	7 cut(s)	179, 256, 1649, 1707, 1894, 2282, 2533
Bse21I	CCTNAGG	1 cut(s)	1090
Bse3DI	GCAATG	3 cut(s)	12, 88, 1416
BseAI	TCCGGA	1 cut(s)	1226
BseBI	CCWGG	2 cut(s)	705, 2702
BseDI	CCNNGG	3 cut(s)	709, 885, 1188
BseGI	GGATG	5 cut(s)	158, 338, 2329, 2595, 2676
BseLI	CCNNNNNNNGG	7 cut(s)	121, 541, 710, 886, 1598, 2456, 2663
BseMI	GCAATG	3 cut(s)	12, 88, 1416
BseMII	CTCAG	3 cut(s)	78, 334, 970
BseNI	ACTGG	7 cut(s)	179, 256, 1649, 1707, 1894, 2282, 2533
BsePI	GCGCGC	1 cut(s)	308
BseRI	GAGGAG	2 cut(s)	1483, 1705
BseXI	GCAGC	7 cut(s)	561, 1036, 1748, 1787, 1893, 2355, 2619
BseYI	CCCAGC	2 cut(s)	1376, 2680
BsgI	GTGCAG	2 cut(s)	151, 2246
Bsh1236I	CGCG	1 cut(s)	310
BshFI	GGCC	9 cut(s)	101, 125, 708, 855, 1146, 1666, 2123, 2231, 2668
BshNI	GGYRCC	1 cut(s)	2055
BshTI	ACCGGT	1 cut(s)	398
BsiHKAI	GWGCWC	5 cut(s)	666, 1262, 1878, 2090, 2523
BsiHKCI	CYCGRG	2 cut(s)	2089, 2627
BsiSI	CCGG	8 cut(s)	180, 237, 399, 456, 471, 1227, 1437, 1904
BslFI	GGGAC	3 cut(s)	220, 1948, 1978
BslI	CCNNNNNNNGG	7 cut(s)	121, 541, 710, 886, 1598, 2456, 2663
BsmAI	GTCTC	3 cut(s)	2212, 2322, 2556
BsmBI	CGTCTC	1 cut(s)	2556
BsmFI	GGGAC	3 cut(s)	220, 1948, 1978
BsmI	GAATGC	1 cut(s)	68
BsnI	GGCC	9 cut(s)	101, 125, 708, 855, 1146, 1666, 2123, 2231, 2668
BsoBI	CYCGRG	2 cut(s)	2089, 2627
Bsp119I	TTCGAA	1 cut(s)	2715
Bsp1286I	GDGCHC	5 cut(s)	666, 1262, 1878, 2090, 2523
Bsp13I	TCCGGA	1 cut(s)	1226
Bsp1407I	TGTACA	4 cut(s)	735, 903, 1394, 2287
Bsp143I	GATC	6 cut(s)	229, 448, 756, 2006, 2076, 2610
Bsp19I	CCATGG	1 cut(s)	1188
BspACI	CCGC	6 cut(s)	324, 335, 1114, 1879, 2059, 2483
BspANI	GGCC	9 cut(s)	101, 125, 708, 855, 1146, 1666, 2123, 2231, 2668
BspCNI	CTCAG	3 cut(s)	77, 333, 969
BspEI	TCCGGA	1 cut(s)	1226
BspFNI	CGCG	1 cut(s)	310
BspLI	GGNNCC	4 cut(s)	149, 256, 1966, 2057
BspMI	ACCTGC	1 cut(s)	946
BspPI	GGATC	2 cut(s)	764, 2014
BspQI	GCTCTTC	2 cut(s)	1866, 2528
BspT104I	TTCGAA	1 cut(s)	2715
BspT107I	GGYRCC	1 cut(s)	2055
BspTI	CTTAAG	1 cut(s)	817
BsrDI	GCAATG	3 cut(s)	12, 88, 1416
BsrFI	RCCGGY	3 cut(s)	398, 455, 470
BsrGI	TGTACA	4 cut(s)	735, 903, 1394, 2287
BsrI	ACTGG	7 cut(s)	179, 256, 1649, 1707, 1894, 2282, 2533
BssAI	RCCGGY	3 cut(s)	398, 455, 470
BssECI	CCNNGG	3 cut(s)	709, 885, 1188
BssHII	GCGCGC	1 cut(s)	308
BssMI	GATC	6 cut(s)	229, 448, 756, 2006, 2076, 2610
BssNAI	GTATAC	1 cut(s)	1942
BssNI	GRCGYC	1 cut(s)	567
BssT1I	CCWWGG	2 cut(s)	885, 1188
Bst1107I	GTATAC	1 cut(s)	1942
Bst2UI	CCWGG	2 cut(s)	705, 2702
Bst4CI	ACNGT	7 cut(s)	478, 523, 672, 692, 1939, 2498, 2831
Bst6I	CTCTTC	6 cut(s)	508, 1449, 1670, 1866, 2009, 2528
BstACI	GRCGYC	1 cut(s)	567
BstAFI	CTTAAG	1 cut(s)	817
BstAPI	GCANNNNNTGC	1 cut(s)	2653
BstAUI	TGTACA	4 cut(s)	735, 903, 1394, 2287
BstBI	TTCGAA	1 cut(s)	2715
BstC8I	GCNNGC	7 cut(s)	43, 190, 310, 457, 828, 2361, 2654
BstDEI	CTNAG	9 cut(s)	64, 320, 956, 1090, 1289, 1547, 1576, 1795, 2405
BstDSI	CCRYGG	1 cut(s)	1188
BstENI	CCTNNNNNAGG	2 cut(s)	884, 2661
BstF5I	GGATG	5 cut(s)	158, 338, 2329, 2595, 2676
BstFNI	CGCG	1 cut(s)	310
BstHHI	GCGC	3 cut(s)	310, 312, 1886
BstKTI	GATC	6 cut(s)	232, 451, 759, 2009, 2079, 2613
BstMAI	GTCTC	3 cut(s)	2212, 2322, 2556
BstMBI	GATC	6 cut(s)	229, 448, 756, 2006, 2076, 2610
BstMWI	GCNNNNNNNGC	7 cut(s)	261, 390, 564, 1800, 2365, 2489, 2653
BstNI	CCWGG	2 cut(s)	705, 2702
BstNSI	RCATGY	1 cut(s)	45
BstPAI	GACNNNNGTC	1 cut(s)	777
BstSCI	CCNGG	3 cut(s)	703, 1902, 2700
BstSFI	CTRYAG	3 cut(s)	1338, 1538, 2433
BstUI	CGCG	1 cut(s)	310
BstV1I	GCAGC	7 cut(s)	561, 1036, 1748, 1787, 1893, 2355, 2619
BstV2I	GAAGAC	1 cut(s)	348
BstX2I	RGATCY	1 cut(s)	2006
BstXI	CCANNNNNNTGG	3 cut(s)	144, 272, 2239
BstYI	RGATCY	1 cut(s)	2006
BstZ17I	GTATAC	1 cut(s)	1942
Bsu36I	CCTNAGG	1 cut(s)	1090
BsuI	GTATCC	1 cut(s)	2432
BsuRI	GGCC	9 cut(s)	101, 125, 708, 855, 1146, 1666, 2123, 2231, 2668
BtgI	CCRYGG	1 cut(s)	1188
BtsCI	GGATG	5 cut(s)	158, 338, 2329, 2595, 2676
BtsI	GCAGTG	2 cut(s)	1983, 2647
BtsIMutI	CAGTG	5 cut(s)	249, 474, 1983, 2526, 2647
BveI	ACCTGC	1 cut(s)	946
Cac8I	GCNNGC	7 cut(s)	43, 190, 310, 457, 828, 2361, 2654
CfoI	GCGC	3 cut(s)	310, 312, 1886
Cfr10I	RCCGGY	3 cut(s)	398, 455, 470
Cfr13I	GGNCC	7 cut(s)	177, 326, 1295, 1439, 1543, 1965, 2666
CseI	GACGC	3 cut(s)	556, 621, 2763
Csp6I	GTAC	6 cut(s)	736, 904, 1319, 1395, 1700, 2288
CspAI	ACCGGT	1 cut(s)	398
CviQI	GTAC	6 cut(s)	736, 904, 1319, 1395, 1700, 2288
DdeI	CTNAG	9 cut(s)	64, 320, 956, 1090, 1289, 1547, 1576, 1795, 2405
DpnI	GATC	6 cut(s)	231, 450, 758, 2008, 2078, 2612
DpnII	GATC	6 cut(s)	229, 448, 756, 2006, 2076, 2610
DraI	TTTAAA	2 cut(s)	2104, 2301
DraIII	CACNNNGTG	1 cut(s)	2085
EaeI	YGGCCR	4 cut(s)	123, 1144, 1664, 2229
Eam1104I	CTCTTC	6 cut(s)	508, 1449, 1670, 1866, 2009, 2528
EarI	CTCTTC	6 cut(s)	508, 1449, 1670, 1866, 2009, 2528
Ecl136II	GAGCTC	3 cut(s)	664, 1876, 2521
Eco130I	CCWWGG	2 cut(s)	885, 1188
Eco147I	AGGCCT	3 cut(s)	101, 708, 855
Eco24I	GRGCYC	3 cut(s)	666, 1878, 2523
Eco32I	GATATC	1 cut(s)	108
Eco47I	GGWCC	6 cut(s)	177, 326, 1295, 1439, 1543, 1965
Eco53kI	GAGCTC	3 cut(s)	664, 1876, 2521
Eco57I	CTGAAG	2 cut(s)	577, 1326
Eco81I	CCTNAGG	1 cut(s)	1090
Eco88I	CYCGRG	2 cut(s)	2089, 2627
EcoICRI	GAGCTC	3 cut(s)	664, 1876, 2521
EcoNI	CCTNNNNNAGG	2 cut(s)	884, 2661
EcoO109I	RGGNCCY	1 cut(s)	1543
EcoRI	GAATTC	1 cut(s)	4
EcoRII	CCWGG	2 cut(s)	703, 2700
EcoRV	GATATC	1 cut(s)	108
EcoT14I	CCWWGG	2 cut(s)	885, 1188
EcoT22I	ATGCAT	2 cut(s)	47, 2494
EcoT38I	GRGCYC	3 cut(s)	666, 1878, 2523
ErhI	CCWWGG	2 cut(s)	885, 1188
Esp3I	CGTCTC	1 cut(s)	2556
FalI	AAGNNNNNCTT	4 cut(s)	86, 118, 1282, 1314
FaqI	GGGAC	3 cut(s)	220, 1948, 1978
FauI	CCCGC	2 cut(s)	342, 2476
FbaI	TGATCA	2 cut(s)	2076, 2610
FblI	GTMKAC	2 cut(s)	60, 1941
Fnu4HI	GCNGC	7 cut(s)	575, 1025, 1737, 1801, 1882, 2369, 2633
FokI	GGATG	5 cut(s)	145, 325, 2336, 2582, 2663
FriOI	GRGCYC	3 cut(s)	666, 1878, 2523
Fsp4HI	GCNGC	7 cut(s)	575, 1025, 1737, 1801, 1882, 2369, 2633
FspBI	CTAG	8 cut(s)	509, 1251, 1322, 1634, 2621, 2636, 2786, 2796
GlaI	GCGC	3 cut(s)	309, 311, 1885
GluI	GCNGC	7 cut(s)	575, 1025, 1737, 1801, 1882, 2369, 2633
GsaI	CCCAGC	2 cut(s)	1380, 2684
HaeIII	GGCC	9 cut(s)	101, 125, 708, 855, 1146, 1666, 2123, 2231, 2668
HapII	CCGG	8 cut(s)	180, 237, 399, 456, 471, 1227, 1437, 1904
HgaI	GACGC	3 cut(s)	556, 621, 2763
HhaI	GCGC	3 cut(s)	310, 312, 1886
Hin1I	GRCGYC	1 cut(s)	567
Hin6I	GCGC	3 cut(s)	308, 310, 1884
HinP1I	GCGC	3 cut(s)	308, 310, 1884
HincII	GTYRAC	3 cut(s)	953, 2827, 2841
HindII	GTYRAC	3 cut(s)	953, 2827, 2841
HindIII	AAGCTT	2 cut(s)	1399, 1502
HpaI	GTTAAC	1 cut(s)	2841
HpaII	CCGG	8 cut(s)	180, 237, 399, 456, 471, 1227, 1437, 1904
HphI	GGTGA	6 cut(s)	388, 460, 511, 557, 782, 1013
Hpy166II	GTNNAC	7 cut(s)	61, 615, 953, 1295, 1942, 2827, 2841
Hpy8I	GTNNAC	7 cut(s)	61, 615, 953, 1295, 1942, 2827, 2841
Hpy99I	CGWCG	1 cut(s)	2549
HpyAV	CCTTC	8 cut(s)	593, 733, 845, 1256, 1295, 1631, 1975, 2657
HpyCH4III	ACNGT	7 cut(s)	478, 523, 672, 692, 1939, 2498, 2831
HpyCH4IV	ACGT	2 cut(s)	2049, 2226
HpyF10VI	GCNNNNNNNGC	7 cut(s)	261, 390, 564, 1800, 2365, 2489, 2653
HpyF3I	CTNAG	9 cut(s)	64, 320, 956, 1090, 1289, 1547, 1576, 1795, 2405
HpySE526I	ACGT	2 cut(s)	2049, 2226
Hsp92I	GRCGYC	1 cut(s)	567
HspAI	GCGC	3 cut(s)	308, 310, 1884
Kpn2I	TCCGGA	1 cut(s)	1226
KroI	GCCGGC	1 cut(s)	455
KroNI	GCCGGC	1 cut(s)	457
Ksp22I	TGATCA	2 cut(s)	2076, 2610
KspAI	GTTAAC	1 cut(s)	2841
Kzo9I	GATC	6 cut(s)	229, 448, 756, 2006, 2076, 2610
LguI	GCTCTTC	2 cut(s)	1866, 2528
LmnI	GCTCC	8 cut(s)	260, 276, 669, 1881, 1926, 2151, 2414, 2518
Lsp1109I	GCAGC	7 cut(s)	561, 1036, 1748, 1787, 1893, 2355, 2619
LweI	GCATC	1 cut(s)	2479
MaeI	CTAG	8 cut(s)	509, 1251, 1322, 1634, 2621, 2636, 2786, 2796
MaeII	ACGT	2 cut(s)	2049, 2226
MaeIII	GTNAC	7 cut(s)	19, 85, 568, 770, 929, 1333, 1861
MalI	GATC	6 cut(s)	231, 450, 758, 2008, 2078, 2612
MboI	GATC	6 cut(s)	229, 448, 756, 2006, 2076, 2610
MfeI	CAATTG	3 cut(s)	831, 1383, 1856
MflI	RGATCY	1 cut(s)	2006
MhlI	GDGCHC	5 cut(s)	666, 1262, 1878, 2090, 2523
MlsI	TGGCCA	3 cut(s)	1146, 1666, 2231
MluNI	TGGCCA	3 cut(s)	1146, 1666, 2231
MlyI	GAGTC	6 cut(s)	56, 202, 240, 875, 2214, 2718
MmeI	TCCRAC	8 cut(s)	691, 699, 828, 1374, 1897, 2496, 2564, 2701
Mox20I	TGGCCA	3 cut(s)	1146, 1666, 2231
Mph1103I	ATGCAT	2 cut(s)	47, 2494
MroI	TCCGGA	1 cut(s)	1226
MroNI	GCCGGC	1 cut(s)	455
MroXI	GAANNNNTTC	2 cut(s)	1222, 1236
MscI	TGGCCA	3 cut(s)	1146, 1666, 2231
MslI	CAYNNNNRTG	2 cut(s)	142, 2237
Msp20I	TGGCCA	3 cut(s)	1146, 1666, 2231
MspA1I	CMGCKG	3 cut(s)	324, 1027, 2483
MspCI	CTTAAG	1 cut(s)	817
MspI	CCGG	8 cut(s)	180, 237, 399, 456, 471, 1227, 1437, 1904
MspR9I	CCNGG	3 cut(s)	705, 1904, 2702
MunI	CAATTG	3 cut(s)	831, 1383, 1856
Mva1269I	GAATGC	1 cut(s)	68
MvaI	CCWGG	2 cut(s)	705, 2702
MvnI	CGCG	1 cut(s)	310
MwoI	GCNNNNNNNGC	7 cut(s)	261, 390, 564, 1800, 2365, 2489, 2653
NaeI	GCCGGC	1 cut(s)	457
NciI	CCSGG	1 cut(s)	1904
NcoI	CCATGG	1 cut(s)	1188
NdeII	GATC	6 cut(s)	229, 448, 756, 2006, 2076, 2610
NgoMIV	GCCGGC	1 cut(s)	455
NlaIV	GGNNCC	4 cut(s)	149, 256, 1966, 2057
NmeAIII	GCCGAG	2 cut(s)	1682, 2331
NmuCI	GTSAC	5 cut(s)	85, 568, 770, 1333, 1861
NsiI	ATGCAT	2 cut(s)	47, 2494
NspI	RCATGY	1 cut(s)	45
NspV	TTCGAA	1 cut(s)	2715
PaeI	GCATGC	1 cut(s)	45
PaeR7I	CTCGAG	1 cut(s)	2089
PauI	GCGCGC	1 cut(s)	308
PceI	AGGCCT	3 cut(s)	101, 708, 855
PciSI	GCTCTTC	2 cut(s)	1866, 2528
PcsI	WCGNNNNNNNCGW	1 cut(s)	664
PctI	GAATGC	1 cut(s)	68
PdiI	GCCGGC	1 cut(s)	457
PdmI	GAANNNNTTC	2 cut(s)	1222, 1236
PfeI	GAWTC	4 cut(s)	198, 376, 655, 916
PflMI	CCANNNNNTGG	1 cut(s)	2456
PinAI	ACCGGT	1 cut(s)	398
PkrI	GCNGC	7 cut(s)	576, 1026, 1738, 1802, 1883, 2370, 2634
PleI	GAGTC	6 cut(s)	56, 202, 240, 874, 2214, 2718
PpsI	GAGTC	6 cut(s)	56, 202, 240, 874, 2214, 2718
PpuMI	RGGWCCY	1 cut(s)	1543
PshAI	GACNNNNGTC	1 cut(s)	777
PshBI	ATTAAT	1 cut(s)	1838
Psp124BI	GAGCTC	3 cut(s)	666, 1878, 2523
Psp5II	RGGWCCY	1 cut(s)	1543
Psp6I	CCWGG	2 cut(s)	703, 2700
PspFI	CCCAGC	2 cut(s)	1376, 2680
PspGI	CCWGG	2 cut(s)	703, 2700
PspN4I	GGNNCC	4 cut(s)	149, 256, 1966, 2057
PspPI	GGNCC	7 cut(s)	177, 326, 1295, 1439, 1543, 1965, 2666
PspPPI	RGGWCCY	1 cut(s)	1543
PspXI	VCTCGAGB	1 cut(s)	2089
PsrI	GAACNNNNNNTAC	2 cut(s)	2374, 2406
PsuI	RGATCY	1 cut(s)	2006
PteI	GCGCGC	1 cut(s)	308
PvuII	CAGCTG	1 cut(s)	1027
RsaI	GTAC	6 cut(s)	737, 905, 1320, 1396, 1701, 2289
RsaNI	GTAC	6 cut(s)	736, 904, 1319, 1395, 1700, 2288
RseI	CAYNNNNRTG	2 cut(s)	142, 2237
SacI	GAGCTC	3 cut(s)	666, 1878, 2523
SapI	GCTCTTC	2 cut(s)	1866, 2528
SatI	GCNGC	7 cut(s)	575, 1025, 1737, 1801, 1882, 2369, 2633
Sau3AI	GATC	6 cut(s)	229, 448, 756, 2006, 2076, 2610
Sau96I	GGNCC	7 cut(s)	177, 326, 1295, 1439, 1543, 1965, 2666
ScaI	AGTACT	2 cut(s)	1320, 1701
SchI	GAGTC	6 cut(s)	56, 202, 240, 875, 2214, 2718
ScrFI	CCNGG	3 cut(s)	705, 1904, 2702
SduI	GDGCHC	5 cut(s)	666, 1262, 1878, 2090, 2523
SfaNI	GCATC	1 cut(s)	2479
SfcI	CTRYAG	3 cut(s)	1338, 1538, 2433
Sfr274I	CTCGAG	1 cut(s)	2089
SfuI	TTCGAA	1 cut(s)	2715
SinI	GGWCC	6 cut(s)	177, 326, 1295, 1439, 1543, 1965
SlaI	CTCGAG	1 cut(s)	2089
SmiMI	CAYNNNNRTG	2 cut(s)	142, 2237
SmlI	CTYRAG	6 cut(s)	52, 817, 1475, 1621, 2089, 2764
SmoI	CTYRAG	6 cut(s)	52, 817, 1475, 1621, 2089, 2764
SphI	GCATGC	1 cut(s)	45
SseBI	AGGCCT	3 cut(s)	101, 708, 855
SsiI	CCGC	6 cut(s)	324, 335, 1114, 1879, 2059, 2483
SspI	AATATT	1 cut(s)	1236
SspMI	CTAG	8 cut(s)	509, 1251, 1322, 1634, 2621, 2636, 2786, 2796
SstI	GAGCTC	3 cut(s)	666, 1878, 2523
StuI	AGGCCT	3 cut(s)	101, 708, 855
StyD4I	CCNGG	3 cut(s)	703, 1902, 2700
StyI	CCWWGG	2 cut(s)	885, 1188
TaaI	ACNGT	7 cut(s)	478, 523, 672, 692, 1939, 2498, 2831
TaiI	ACGT	2 cut(s)	2052, 2229
TaqI	TCGA	7 cut(s)	228, 658, 919, 1496, 2090, 2477, 2715
TaqII	GACCGA	1 cut(s)	1982
TatI	WGTACW	6 cut(s)	735, 903, 1318, 1394, 1699, 2287
TfiI	GAWTC	4 cut(s)	198, 376, 655, 916
TscAI	CASTG	5 cut(s)	256, 481, 1983, 2533, 2654
TseFI	GTSAC	5 cut(s)	85, 568, 770, 1333, 1861
TseI	GCWGC	7 cut(s)	574, 1024, 1736, 1800, 1881, 2368, 2632
Tsp45I	GTSAC	5 cut(s)	85, 568, 770, 1333, 1861
TspDTI	ATGAA	6 cut(s)	17, 778, 1118, 1149, 1405, 2676
TspGWI	ACGGA	2 cut(s)	1230, 2766
TspRI	CASTG	5 cut(s)	256, 481, 1983, 2533, 2654
Van91I	CCANNNNNTGG	1 cut(s)	2456
Vha464I	CTTAAG	1 cut(s)	817
VpaK11BI	GGWCC	6 cut(s)	177, 326, 1295, 1439, 1543, 1965
VspI	ATTAAT	1 cut(s)	1838
XagI	CCTNNNNNAGG	2 cut(s)	884, 2661
XapI	RAATTY	7 cut(s)	4, 549, 624, 921, 1270, 1894, 1909
XbaI	TCTAGA	1 cut(s)	1633
XceI	RCATGY	1 cut(s)	45
XhoI	CTCGAG	1 cut(s)	2089
XmiI	GTMKAC	2 cut(s)	60, 1941
XmnI	GAANNNNTTC	2 cut(s)	1222, 1236
XspI	CTAG	8 cut(s)	509, 1251, 1322, 1634, 2621, 2636, 2786, 2796
ZrmI	AGTACT	2 cut(s)	1320, 1701
Zsp2I	ATGCAT	2 cut(s)	47, 2494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.