Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0005533

Physical Location & Seq

Genomic Coordinates Forward (+)

4265264 .. 4268174

Gene Structure 2911 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g06980.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2715 bp

ATGGATTTCCACTTCCATCTATCGCCACAACTTTCCGCATTTTCTATTCTTCTGACATGCTTTTTTGTGAGCATAGAGTCAGTAATTACAAGCATTTCCACAGACAAAGAAGCCTTGATCTCATTCAAATCAACAGCCAATCTCCCTCCATATTTCTGGGACCAAAGCTCATCCCCGTGTACCAACTGGGCTGGAGTTGTATGCAACAAACATGGCAAGAGAGTCATTGCTCTTGACCTCTCAGGCTTGGGACTCACAGGCTCCATAAGCCCTCACATTGGTAACCTCTCCTTCCTCCATTCCCTCCAACTCCATAACAACAAATTAACCGGAACAATTCCCACTCAAATTTTCAACCTTTTTCGCTTGAGGTCGCTCAACGTAAGCTCCAACACCCTACGAGGTTCACTTCCATCAAACATAACCAAATTGATGGCACTCGAAATCCTCGACTTGTCCTCAAACAACATCACAGGAATCCTCCCGCAAAACCTTAGACAGTTGAAAAATCTCCAAGTCTTGAATTTGGCACGAAACAATTTTTTCGGTCCGATCCCACCATCGATTAGCAACCTTTCATCCACCCTCACAAATTTGAATCTAGGTACAAACAGACTCAGTGGTACTATACCTAGTGAATTAGGTCACCTCCATAAGCTCAAAGAACTCGACCTCGCCGTTAACCAACTCACCGGGACCATTGCCTCATCCATATACAACATTTCCTCTCTAGTTTTGTTCACTGTGGCTTCAAACCATCTGTGGGGTGAGATTCCTAGCAATATTGATGACACACTTCCAAATCTTTTGTACTTCAGGAATTGTATCAATCAATTCACAGGAAAAATTCCTCCTTCTCTACACAACATCTCTGGACTTCGATCAATTCGTATGTCTAACAACTTTCTTGAAGGAACAGTTCCACCGGGCCTCGGAAATCTTCCCTTCCTCGAAATGTACAACATTGGCTTCAATCGGATAGTTAGCTACGGTGACAATGGTCTTAATTTCATTACTGCTTTGACAAACAGCAGTCGCTTAAGTTGGCTTGCGATTGATGAAAATCGGCTGGAAGGCCTAATTCCAGAGTCCATTGGCAACCTCTCCAAGGTTCTGTCAAAATTGTACATGGGAGGGAATCGAATTTATGGTAACATACCTGCCTCAATTAGTCAACTCAGTAGTTTAACTTTGCTAAATGTGAGCTACAATTCAATTTCTGGTGAAATCCCATCAGAAATTGGGCGGCTGAAAGAGTTGCAAATGCTTGGTTTAGCAAGAAATAAATTGTCTGGCACAATTCCAAACTCCTTAGGTAATATCATGAAATTGAACCATATTGATTTATCTGGAAACTACTTTGTTGGCCATATACCCCTTTCATTTTCAAACTTCCAGAAATTGCTTTCCATGGACTTATCCGACAATTTGCTCAACGGAAGTATTTCCGGAGAAATATTTCTAAACCTTCCTAGTTTGAGCACTATTCTCAATCTGTCTAACAATTTCCTGAGTGGACCTTTGCCTGAAGAATTAGGATCTCTCGCAAGTGTTGCCACTATTGACCTCTCTAGCAACCAGTTTTCGGGGTTGATTCCCAGCTCAATTGGAAAGTGTACAAGCTTGGACGGATTGTTCATGGCCAGAAACAAACTTTCAGGTCCCCTTCCAAATGCTCTTGGAGAAATGAGGGGTCTTGAGATTTTAGACCTCTCCTCGAACCAACTATCTGGCTTTATTCCTGACAAACTTCAAGACCTGCGTGTCCTTCGATACTTGAACCTCTCATTTAATAACTTAGAAGGAGTGATTCCAAATGGTGGAGTTTTTAAAAATATCTCAAGTGTCCATCTTGAAGGCAACCCTAAGCTATGCATGCATTTCCCATGTGTGAAACGTAGGAGAAAGGTACTTGTCCGAGTTCTTATCTCTACTGTTGCTTTGGCAACGTTGGTAACATGTATTACAGTTGGTCTTTTGCTACATTTTAGAAAAAGAAGAGGCTCAAGCGGCAAAGCAACAGAAAATAATTCTGGTTTGCTACTAAAAGGGCAACATCAAATGGTCAGTTACGAAGAGCTACGTGGGGCAACTGGGAATTTCAACCCCGAAAATTTGATTGGAACAGGGAGCTTTGGGGCTGTATACAAAGGTCTTTTGAGAGAAGGAACTGAAGTTGCAGTGAAGGTGCTTGATATGAAAATGACAGCATCTTGGAAGAGTTTTATGGCAGAGTGCGAAGCATTGAGGAGCGTGAGGCACCGGAATCTTGTTAAACTGATCAACTCTTGCTCCAGCTTGGACTTTAAAAACATGGAGTTTCTTGCTTTGGTTTACGAGTATTTGAGTAATGGGAGCTTGGAAGATTGGATTAGAGGCAAGAGAAAAAATGCAGATGGAGATGGGTTGAACATTTTTGAGAGACTAAATGTGGCCATTGATGTGGCGTGTGGATTGGATTATCTGCATCATGACTGCGAAGTTCCGGTTGTGCACTGCGATTTAAAGCCGAGCAACATTCTTTTGGATGGAGATATGAATGCAAAGATCGGAGATTTCGGGCTGGCGAAGCTGCTAATGGAGAGTACAGAGATTGAGCAACAGTCACTTAGCTTCACACATGTCCTCAAGGGTTCTATAGGATACAAACCCCCAGGTTATATGGTATCCACCAGAAACCCTCGACAGCGGGTGATGCATACAGTTTCGGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008219 GO:0009595 GO:0009605 GO:0009607 GO:0009617 GO:0009626 GO:0009987 GO:0010204 GO:0010359 GO:0012501 GO:0012505 GO:0016020 GO:0016045 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022898 GO:0023052 GO:0031347 GO:0031349 GO:0032409 GO:0032412 GO:0032879 GO:0033554 GO:0034050 GO:0034762 GO:0034765 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043269 GO:0043412 GO:0044070 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051606 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0065009 GO:0071704 GO:0071944 GO:0080134 GO:0098543 GO:0098581 GO:0140096 GO:1901564 GO:1903959

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

905

Amino Acids

98.98

Weight (kDa)

8.78

Isoelectric Point (pI)

41.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	33 - 69	7.1e-07	Leucine rich repeat N-terminal domain
LRR_14	PF23598	89 - 257	1.1e-14	Leucine-rich repeat region
LRR_8	PF13855	122 - 181	6.3e-08	Leucine rich repeat
LRR_4	PF12799	147 - 187	5.6e-06	Leucine Rich repeats (2 copies)
LRR_14	PF23598	340 - 449	5.7e-08	Leucine-rich repeat region
LRR_14	PF23598	460 - 600	1.4e-09	Leucine-rich repeat region
LRR_8	PF13855	560 - 600	1.1e-07	Leucine rich repeat
Pkinase	PF00069	703 - 887	1.3e-31	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	705 - 867	8.2e-30	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005533)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g36850 FvH4_3g36850 FvH4_3g36850
malus_domestica	MD08G1090900.v1.1 MD15G1074900.v1.1 MD15G1075000.v1.1
prunus_persica	Prupe.1G427400_v2.0.a1 Prupe.1G427700_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1 Prupe.1G427800_v2.0.a1
pyrus_communis	pycom15g06980 pycom15g06990
rosa_chinensis	RchiOBHm_Chr5g0066001 RchiOBHm_Chr5g0066021
rosa_laevigata	RLG00000035833 RLG00000035834
rosa_multiflora	Rmu_sc0001473.1_g000006 Rmu_sc0011576.1_g000012 Rmu_sc0011576.1_g000015
rosa_roxburghii	Rroxscaffold_1G00014980 Rroxscaffold_1G00015120
rosa_rugosa	Rorug05G0375800 Rorug05G0375900 Rorug05G0376000
rosa_samantha	Rh5AG434300 Rh5AG434500 Rh5CG472900
rosa_wichuraiana	Rw5G040690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1763
Acc36I	ACCTGC	2 cut(s)	1168, 1767
AccB1I	GGYRCC	1 cut(s)	2259
AccB7I	CCANNNNNTGG	1 cut(s)	1820
AccI	GTMKAC	1 cut(s)	2145
AccIII	TCCGGA	1 cut(s)	1448
AciI	CCGC	5 cut(s)	36, 485, 1246, 2010, 2689
AclI	AACGTT	1 cut(s)	1949
AclWI	GGATC	2 cut(s)	547, 1546
AcoI	YGGCCR	3 cut(s)	1366, 1641, 2433
AcsI	RAATTY	7 cut(s)	348, 523, 592, 846, 1143, 2098, 2113
AcuI	CTGAAG	3 cut(s)	799, 1548, 2193
AfaI	GTAC	9 cut(s)	181, 607, 625, 812, 959, 1127, 1618, 1911, 2587
AfiI	CCNNNNNNNGG	6 cut(s)	278, 763, 932, 1108, 1585, 1820
AflII	CTTAAG	1 cut(s)	1039
AflIII	ACRYGT	2 cut(s)	1958, 2620
AjnI	CCWGG	1 cut(s)	2653
AjuI	GAANNNNNNNTTGG	2 cut(s)	2103, 2135
AloI	GAACNNNNNNTCC	2 cut(s)	1619, 1651
Alw21I	GWGCWC	2 cut(s)	1484, 2496
Alw26I	GTCTC	1 cut(s)	2416
Alw44I	GTGCAC	1 cut(s)	2492
AlwI	GGATC	2 cut(s)	547, 1546
Aor13HI	TCCGGA	1 cut(s)	1448
AoxI	GGCC	5 cut(s)	928, 1075, 1366, 1641, 2433
ApaLI	GTGCAC	1 cut(s)	2492
ApeKI	GCWGC	1 cut(s)	2572
ApoI	RAATTY	7 cut(s)	348, 523, 592, 846, 1143, 2098, 2113
Asp700I	GAANNNNTTC	2 cut(s)	1444, 1458
AspS9I	GGNCC	6 cut(s)	160, 548, 696, 928, 1517, 1661
AsuC2I	CCSGG	2 cut(s)	694, 927
AsuHPI	GGTGA	6 cut(s)	638, 682, 779, 1004, 1235, 2704
AvaII	GGWCC	5 cut(s)	160, 548, 696, 1517, 1661
AxyI	CCTNAGG	1 cut(s)	1312
BaeGI	GKGCMC	1 cut(s)	2496
BalI	TGGCCA	3 cut(s)	1368, 1643, 2435
BanI	GGYRCC	1 cut(s)	2259
Bbv12I	GWGCWC	2 cut(s)	1484, 2496
BbvI	GCAGC	1 cut(s)	2559
BceAI	ACGGC	1 cut(s)	662
BciT130I	CCWGG	1 cut(s)	2655
BciVI	GTATCC	2 cut(s)	2636, 2677
BclI	TGATCA	1 cut(s)	2280
BcnI	CCSGG	2 cut(s)	694, 927
BcoDI	GTCTC	1 cut(s)	2416
BfaI	CTAG	6 cut(s)	602, 633, 731, 777, 1473, 1572
BfmI	CTRYAG	1 cut(s)	2637
BfrI	CTTAAG	1 cut(s)	1039
BfuAI	ACCTGC	2 cut(s)	1168, 1767
BfuI	GTATCC	2 cut(s)	2636, 2677
BisI	GCNGC	3 cut(s)	1247, 2011, 2573
BlsI	GCNGC	3 cut(s)	1248, 2012, 2574
Bme1390I	CCNGG	3 cut(s)	694, 927, 2655
Bme18I	GGWCC	5 cut(s)	160, 548, 696, 1517, 1661
BmgT120I	GGNCC	6 cut(s)	160, 548, 696, 928, 1517, 1661
BmiI	GGNNCC	5 cut(s)	161, 262, 697, 1663, 2261
BmrFI	CCNGG	3 cut(s)	694, 927, 2655
BmrI	ACTGGG	2 cut(s)	196, 2103
BmsI	GCATC	3 cut(s)	2219, 2476, 2685
BmuI	ACTGGG	2 cut(s)	196, 2103
BoxI	GACNNNNGTC	1 cut(s)	999
BpmI	CTGGAG	2 cut(s)	213, 2278
Bpu10I	CCTNAGC	1 cut(s)	1866
BpuEI	CTTGAG	5 cut(s)	388, 1718, 1825, 1990, 2612
BpuMI	CCSGG	2 cut(s)	694, 927
Bsa29I	ATCGAT	1 cut(s)	563
BsaAI	YACGTR	1 cut(s)	2084
BsaBI	GATNNNNATC	1 cut(s)	1062
BsaJI	CCNNGG	4 cut(s)	931, 1107, 1410, 2653
BsaWI	WCCGGW	4 cut(s)	329, 1448, 2262, 2485
BsaXI	ACNNNNNCTCC	4 cut(s)	371, 401, 2276, 2306
Bsc4I	CCNNNNNNNGG	6 cut(s)	278, 763, 932, 1108, 1585, 1820
Bse1I	ACTGG	3 cut(s)	191, 1579, 2098
Bse21I	CCTNAGG	1 cut(s)	1312
Bse3DI	GCAATG	2 cut(s)	225, 699
Bse8I	GATNNNNATC	1 cut(s)	1062
BseAI	TCCGGA	1 cut(s)	1448
BseBI	CCWGG	1 cut(s)	2655
BseCI	ATCGAT	1 cut(s)	563
BseDI	CCNNGG	4 cut(s)	931, 1107, 1410, 2653
BseGI	GGATG	4 cut(s)	170, 578, 707, 2533
BseJI	GATNNNNATC	1 cut(s)	1062
BseLI	CCNNNNNNNGG	6 cut(s)	278, 763, 932, 1108, 1585, 1820
BseMI	GCAATG	2 cut(s)	225, 699
BseMII	CTCAG	4 cut(s)	255, 631, 1192, 1502
BseNI	ACTGG	3 cut(s)	191, 1579, 2098
BseRI	GAGGAG	2 cut(s)	1705, 2263
BseSI	GKGCMC	1 cut(s)	2496
BseXI	GCAGC	1 cut(s)	2559
BseYI	CCCAGC	1 cut(s)	1598
BshFI	GGCC	5 cut(s)	930, 1077, 1368, 1643, 2435
BshNI	GGYRCC	1 cut(s)	2259
BshVI	ATCGAT	1 cut(s)	563
BsiHKAI	GWGCWC	2 cut(s)	1484, 2496
BsiSI	CCGG	6 cut(s)	330, 693, 926, 1449, 2263, 2486
BslFI	GGGAC	4 cut(s)	173, 264, 709, 1647
BslI	CCNNNNNNNGG	6 cut(s)	278, 763, 932, 1108, 1585, 1820
BsmAI	GTCTC	1 cut(s)	2416
BsmFI	GGGAC	4 cut(s)	173, 264, 709, 1647
BsmI	GAATGC	1 cut(s)	2545
BsnI	GGCC	5 cut(s)	930, 1077, 1368, 1643, 2435
Bsp1286I	GDGCHC	2 cut(s)	1484, 2496
Bsp13I	TCCGGA	1 cut(s)	1448
Bsp1407I	TGTACA	3 cut(s)	957, 1125, 1616
Bsp143I	GATC	6 cut(s)	117, 552, 881, 1538, 2280, 2547
Bsp19I	CCATGG	1 cut(s)	1410
BspACI	CCGC	5 cut(s)	36, 485, 1246, 2010, 2689
BspANI	GGCC	5 cut(s)	930, 1077, 1368, 1643, 2435
BspCNI	CTCAG	4 cut(s)	254, 630, 1191, 1503
BspDI	ATCGAT	1 cut(s)	563
BspEI	TCCGGA	1 cut(s)	1448
BspHI	TCATGA	2 cut(s)	1323, 2470
BspLI	GGNNCC	5 cut(s)	161, 262, 697, 1663, 2261
BspMI	ACCTGC	2 cut(s)	1168, 1767
BspPI	GGATC	2 cut(s)	547, 1546
BspQI	GCTCTTC	1 cut(s)	2070
BspT107I	GGYRCC	1 cut(s)	2259
BspTI	CTTAAG	1 cut(s)	1039
BsrDI	GCAATG	2 cut(s)	225, 699
BsrGI	TGTACA	3 cut(s)	957, 1125, 1616
BsrI	ACTGG	3 cut(s)	191, 1579, 2098
BssECI	CCNNGG	4 cut(s)	931, 1107, 1410, 2653
BssMI	GATC	6 cut(s)	117, 552, 881, 1538, 2280, 2547
BssNAI	GTATAC	1 cut(s)	2146
BssT1I	CCWWGG	2 cut(s)	1107, 1410
Bst1107I	GTATAC	1 cut(s)	2146
Bst2UI	CCWGG	1 cut(s)	2655
Bst4CI	ACNGT	8 cut(s)	501, 745, 919, 992, 1936, 1969, 2604, 2704
Bst6I	CTCTTC	3 cut(s)	1993, 2070, 2213
BstAFI	CTTAAG	1 cut(s)	1039
BstAPI	GCANNNNNTGC	1 cut(s)	1553
BstAUI	TGTACA	3 cut(s)	957, 1125, 1616
BstBAI	YACGTR	1 cut(s)	2084
BstC8I	GCNNGC	3 cut(s)	1050, 1877, 2565
BstDEI	CTNAG	9 cut(s)	241, 494, 617, 1178, 1312, 1511, 1798, 1866, 2609
BstDSI	CCRYGG	1 cut(s)	1410
BstEII	GGTNACC	2 cut(s)	281, 644
BstENI	CCTNNNNNAGG	1 cut(s)	1106
BstF5I	GGATG	4 cut(s)	170, 578, 707, 2533
BstKTI	GATC	6 cut(s)	120, 555, 884, 1541, 2283, 2550
BstMAI	GTCTC	1 cut(s)	2416
BstMBI	GATC	6 cut(s)	117, 552, 881, 1538, 2280, 2547
BstMWI	GCNNNNNNNGC	6 cut(s)	267, 1553, 1876, 2010, 2569, 2695
BstNI	CCWGG	1 cut(s)	2655
BstNSI	RCATGY	4 cut(s)	60, 1879, 1962, 2624
BstPAI	GACNNNNGTC	1 cut(s)	999
BstPI	GGTNACC	2 cut(s)	281, 644
BstSCI	CCNGG	3 cut(s)	692, 925, 2653
BstSFI	CTRYAG	1 cut(s)	2637
BstSLI	GKGCMC	1 cut(s)	2496
BstV1I	GCAGC	1 cut(s)	2559
BstX2I	RGATCY	1 cut(s)	1538
BstXI	CCANNNNNNTGG	4 cut(s)	156, 433, 1730, 2443
BstYI	RGATCY	1 cut(s)	1538
BstZ17I	GTATAC	1 cut(s)	2146
Bsu15I	ATCGAT	1 cut(s)	563
Bsu36I	CCTNAGG	1 cut(s)	1312
BsuI	GTATCC	2 cut(s)	2636, 2677
BsuRI	GGCC	5 cut(s)	930, 1077, 1368, 1643, 2435
BsuTUI	ATCGAT	1 cut(s)	563
BtgI	CCRYGG	1 cut(s)	1410
BtsCI	GGATG	4 cut(s)	170, 578, 707, 2533
BtsI	GCAGTG	2 cut(s)	2187, 2494
BtsIMutI	CAGTG	4 cut(s)	625, 741, 2187, 2494
BveI	ACCTGC	2 cut(s)	1168, 1767
Cac8I	GCNNGC	3 cut(s)	1050, 1877, 2565
CciI	TCATGA	2 cut(s)	1323, 2470
Cfr13I	GGNCC	6 cut(s)	160, 548, 696, 928, 1517, 1661
ClaI	ATCGAT	1 cut(s)	563
CpoI	CGGWCCG	1 cut(s)	548
Csp6I	GTAC	9 cut(s)	180, 606, 624, 811, 958, 1126, 1617, 1910, 2586
CspI	CGGWCCG	1 cut(s)	548
CviQI	GTAC	9 cut(s)	180, 606, 624, 811, 958, 1126, 1617, 1910, 2586
DdeI	CTNAG	9 cut(s)	241, 494, 617, 1178, 1312, 1511, 1798, 1866, 2609
DpnI	GATC	6 cut(s)	119, 554, 883, 1540, 2282, 2549
DpnII	GATC	6 cut(s)	117, 552, 881, 1538, 2280, 2547
DraI	TTTAAA	3 cut(s)	1831, 2308, 2505
DrdI	GACNNNNNNGTC	1 cut(s)	1763
DseDI	GACNNNNNNGTC	1 cut(s)	1763
EaeI	YGGCCR	3 cut(s)	1366, 1641, 2433
Eam1104I	CTCTTC	3 cut(s)	1993, 2070, 2213
EarI	CTCTTC	3 cut(s)	1993, 2070, 2213
Eco130I	CCWWGG	2 cut(s)	1107, 1410
Eco147I	AGGCCT	1 cut(s)	1077
Eco47I	GGWCC	5 cut(s)	160, 548, 696, 1517, 1661
Eco57I	CTGAAG	3 cut(s)	799, 1548, 2193
Eco81I	CCTNAGG	1 cut(s)	1312
Eco91I	GGTNACC	2 cut(s)	281, 644
EcoNI	CCTNNNNNAGG	1 cut(s)	1106
EcoO109I	RGGNCCY	1 cut(s)	1661
EcoO65I	GGTNACC	2 cut(s)	281, 644
EcoRII	CCWGG	1 cut(s)	2653
EcoT14I	CCWWGG	2 cut(s)	1107, 1410
EcoT22I	ATGCAT	3 cut(s)	1877, 1881, 2700
ErhI	CCWWGG	2 cut(s)	1107, 1410
FaqI	GGGAC	4 cut(s)	173, 264, 709, 1647
FauI	CCCGC	2 cut(s)	492, 2682
FbaI	TGATCA	1 cut(s)	2280
FblI	GTMKAC	1 cut(s)	2145
Fnu4HI	GCNGC	3 cut(s)	1247, 2011, 2573
FokI	GGATG	4 cut(s)	157, 565, 694, 2540
Fsp4HI	GCNGC	3 cut(s)	1247, 2011, 2573
FspBI	CTAG	6 cut(s)	602, 633, 731, 777, 1473, 1572
GluI	GCNGC	3 cut(s)	1247, 2011, 2573
GsaI	CCCAGC	1 cut(s)	1602
GsuI	CTGGAG	2 cut(s)	213, 2278
HaeIII	GGCC	5 cut(s)	930, 1077, 1368, 1643, 2435
HapII	CCGG	6 cut(s)	330, 693, 926, 1449, 2263, 2486
HincII	GTYRAC	2 cut(s)	682, 1175
HindII	GTYRAC	2 cut(s)	682, 1175
HindIII	AAGCTT	1 cut(s)	1621
HpaI	GTTAAC	1 cut(s)	682
HpaII	CCGG	6 cut(s)	330, 693, 926, 1449, 2263, 2486
HphI	GGTGA	6 cut(s)	638, 682, 779, 1004, 1235, 2704
Hpy188I	TCNGA	9 cut(s)	54, 552, 935, 978, 1237, 1423, 1919, 2552, 2710
HpyCH4III	ACNGT	8 cut(s)	501, 745, 919, 992, 1936, 1969, 2604, 2704
HpyCH4IV	ACGT	4 cut(s)	381, 1897, 1949, 2083
HpyF10VI	GCNNNNNNNGC	6 cut(s)	267, 1553, 1876, 2010, 2569, 2695
HpyF3I	CTNAG	9 cut(s)	241, 494, 617, 1178, 1312, 1511, 1798, 1866, 2609
HpySE526I	ACGT	4 cut(s)	381, 1897, 1949, 2083
Kpn2I	TCCGGA	1 cut(s)	1448
Ksp22I	TGATCA	1 cut(s)	2280
KspAI	GTTAAC	1 cut(s)	682
Kzo9I	GATC	6 cut(s)	117, 552, 881, 1538, 2280, 2547
LguI	GCTCTTC	1 cut(s)	2070
LmnI	GCTCC	6 cut(s)	266, 392, 2130, 2250, 2297, 2355
Lsp1109I	GCAGC	1 cut(s)	2559
LweI	GCATC	3 cut(s)	2219, 2476, 2685
MaeI	CTAG	6 cut(s)	602, 633, 731, 777, 1473, 1572
MaeII	ACGT	4 cut(s)	381, 1897, 1949, 2083
MaeIII	GTNAC	7 cut(s)	281, 644, 992, 1151, 1954, 2069, 2604
MalI	GATC	6 cut(s)	119, 554, 883, 1540, 2282, 2549
MboI	GATC	6 cut(s)	117, 552, 881, 1538, 2280, 2547
MboII	GAAGA	7 cut(s)	41, 932, 1541, 2010, 2087, 2230, 2375
MfeI	CAATTG	1 cut(s)	1605
MflI	RGATCY	1 cut(s)	1538
MhlI	GDGCHC	2 cut(s)	1484, 2496
MlsI	TGGCCA	3 cut(s)	1368, 1643, 2435
MluNI	TGGCCA	3 cut(s)	1368, 1643, 2435
MlyI	GAGTC	5 cut(s)	86, 231, 246, 609, 1097
MmeI	TCCRAC	3 cut(s)	331, 414, 1446
Mox20I	TGGCCA	3 cut(s)	1368, 1643, 2435
Mph1103I	ATGCAT	3 cut(s)	1877, 1881, 2700
MroI	TCCGGA	1 cut(s)	1448
MroXI	GAANNNNTTC	2 cut(s)	1444, 1458
MscI	TGGCCA	3 cut(s)	1368, 1643, 2435
MslI	CAYNNNNRTG	2 cut(s)	175, 2441
Msp20I	TGGCCA	3 cut(s)	1368, 1643, 2435
MspA1I	CMGCKG	1 cut(s)	2689
MspCI	CTTAAG	1 cut(s)	1039
MspI	CCGG	6 cut(s)	330, 693, 926, 1449, 2263, 2486
MspR9I	CCNGG	3 cut(s)	694, 927, 2655
MunI	CAATTG	1 cut(s)	1605
Mva1269I	GAATGC	1 cut(s)	2545
MvaI	CCWGG	1 cut(s)	2655
MwoI	GCNNNNNNNGC	6 cut(s)	267, 1553, 1876, 2010, 2569, 2695
NciI	CCSGG	2 cut(s)	694, 927
NcoI	CCATGG	1 cut(s)	1410
NdeII	GATC	6 cut(s)	117, 552, 881, 1538, 2280, 2547
NlaIV	GGNNCC	5 cut(s)	161, 262, 697, 1663, 2261
NmeAIII	GCCGAG	1 cut(s)	2535
NmuCI	GTSAC	3 cut(s)	644, 992, 2604
NsiI	ATGCAT	3 cut(s)	1877, 1881, 2700
NspI	RCATGY	4 cut(s)	60, 1879, 1962, 2624
PaeI	GCATGC	1 cut(s)	1879
PagI	TCATGA	2 cut(s)	1323, 2470
PceI	AGGCCT	1 cut(s)	1077
PciI	ACATGT	2 cut(s)	1958, 2620
PciSI	GCTCTTC	1 cut(s)	2070
PcsI	WCGNNNNNNNCGW	3 cut(s)	447, 675, 886
PctI	GAATGC	1 cut(s)	2545
PdmI	GAANNNNTTC	2 cut(s)	1444, 1458
PfeI	GAWTC	7 cut(s)	477, 598, 772, 1138, 1594, 1810, 2266
PflMI	CCANNNNNTGG	1 cut(s)	1820
PkrI	GCNGC	3 cut(s)	1248, 2012, 2574
PleI	GAGTC	5 cut(s)	85, 230, 246, 609, 1096
PpsI	GAGTC	5 cut(s)	85, 230, 246, 609, 1096
Ppu21I	YACGTR	1 cut(s)	2084
PpuMI	RGGWCCY	1 cut(s)	1661
PscI	ACATGT	2 cut(s)	1958, 2620
PshAI	GACNNNNGTC	1 cut(s)	999
Psp1406I	AACGTT	1 cut(s)	1949
Psp5II	RGGWCCY	1 cut(s)	1661
Psp6I	CCWGG	1 cut(s)	2653
PspEI	GGTNACC	2 cut(s)	281, 644
PspFI	CCCAGC	1 cut(s)	1598
PspGI	CCWGG	1 cut(s)	2653
PspN4I	GGNNCC	5 cut(s)	161, 262, 697, 1663, 2261
PspPI	GGNCC	6 cut(s)	160, 548, 696, 928, 1517, 1661
PspPPI	RGGWCCY	1 cut(s)	1661
PsuI	RGATCY	1 cut(s)	1538
RsaI	GTAC	9 cut(s)	181, 607, 625, 812, 959, 1127, 1618, 1911, 2587
RsaNI	GTAC	9 cut(s)	180, 606, 624, 811, 958, 1126, 1617, 1910, 2586
RseI	CAYNNNNRTG	2 cut(s)	175, 2441
Rsr2I	CGGWCCG	1 cut(s)	548
RsrII	CGGWCCG	1 cut(s)	548
SapI	GCTCTTC	1 cut(s)	2070
SatI	GCNGC	3 cut(s)	1247, 2011, 2573
Sau3AI	GATC	6 cut(s)	117, 552, 881, 1538, 2280, 2547
Sau96I	GGNCC	6 cut(s)	160, 548, 696, 928, 1517, 1661
SchI	GAGTC	5 cut(s)	86, 231, 246, 609, 1097
ScrFI	CCNGG	3 cut(s)	694, 927, 2655
SduI	GDGCHC	2 cut(s)	1484, 2496
SfaNI	GCATC	3 cut(s)	2219, 2476, 2685
SfcI	CTRYAG	1 cut(s)	2637
SinI	GGWCC	5 cut(s)	160, 548, 696, 1517, 1661
SmiMI	CAYNNNNRTG	2 cut(s)	175, 2441
SmlI	CTYRAG	6 cut(s)	367, 1039, 1697, 1840, 2005, 2627
SmoI	CTYRAG	6 cut(s)	367, 1039, 1697, 1840, 2005, 2627
SphI	GCATGC	1 cut(s)	1879
SseBI	AGGCCT	1 cut(s)	1077
SsiI	CCGC	5 cut(s)	36, 485, 1246, 2010, 2689
SspI	AATATT	2 cut(s)	784, 1458
SspMI	CTAG	6 cut(s)	602, 633, 731, 777, 1473, 1572
StuI	AGGCCT	1 cut(s)	1077
StyD4I	CCNGG	3 cut(s)	692, 925, 2653
StyI	CCWWGG	2 cut(s)	1107, 1410
TaaI	ACNGT	8 cut(s)	501, 745, 919, 992, 1936, 1969, 2604, 2704
TaiI	ACGT	4 cut(s)	384, 1900, 1952, 2086
TaqII	GACCGA	1 cut(s)	536
TatI	WGTACW	5 cut(s)	810, 957, 1125, 1616, 2585
TauI	GCSGC	2 cut(s)	1249, 2013
TfiI	GAWTC	7 cut(s)	477, 598, 772, 1138, 1594, 1810, 2266
TscAI	CASTG	4 cut(s)	625, 748, 2187, 2501
TseFI	GTSAC	3 cut(s)	644, 992, 2604
TseI	GCWGC	1 cut(s)	2572
Tsp45I	GTSAC	3 cut(s)	644, 992, 2604
TspDTI	ATGAA	8 cut(s)	567, 1000, 1074, 1340, 1371, 1627, 2213, 2552
TspGWI	ACGGA	2 cut(s)	1452, 1644
TspRI	CASTG	4 cut(s)	625, 748, 2187, 2501
Van91I	CCANNNNNTGG	1 cut(s)	1820
Vha464I	CTTAAG	1 cut(s)	1039
VneI	GTGCAC	1 cut(s)	2492
VpaK11BI	GGWCC	5 cut(s)	160, 548, 696, 1517, 1661
XagI	CCTNNNNNAGG	1 cut(s)	1106
XapI	RAATTY	7 cut(s)	348, 523, 592, 846, 1143, 2098, 2113
XceI	RCATGY	4 cut(s)	60, 1879, 1962, 2624
XmiI	GTMKAC	1 cut(s)	2145
XmnI	GAANNNNTTC	2 cut(s)	1444, 1458
XspI	CTAG	6 cut(s)	602, 633, 731, 777, 1473, 1572
Zsp2I	ATGCAT	3 cut(s)	1877, 1881, 2700

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.