FvH4_3g42370

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006832
Physical Location & Seq
Forward (+)
35373815 .. 35381571
7757 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42370.t1

Sequence Viewer

Length: 4044 bp
ATGGACACGAATAATTGGAGGCCTCCTCCGGGTGGGGAGCCTGCAATGGATGCAGGAGATTGGAGGAGCCAGTTGCAGGCGGATTCGCGGCAGAGAATCGTTAATAAGATAATGGATACATTGAAGAGGCATCTTCCTTTCTCCGGCCAAGAGGGACTGCATGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAGAAGATTTATACTGCTGCTACGAGCCAGTCGGATTATCTACGGAAAATTTCTCTGAAGATGCTCACTATGGAGAGCAAGTCTCAGAACACCATGGGTAATTCTTTACCAAGCAACTCTGCGGGTAATAGTAACAGGCCCCCTGATCCAGGATCTTTCGGTATGCAAGCCCAAGCTCCGAATCAAGGACAGTCACTCTCTATGCCAGTGCAAGCCAATCAATCTCAAACACGACAACAACTGTTATCACAGAACATTCAGAATAACATTCCGCCCACTGGAGTTCAAAATTCTGCTGGGTTATCATCTGCGCTACCTCCTGCATCAGGTCTCACCCAGACTCCTATCCCGAATATGGTTGGACAAAGTGCAAACTTGCAAAGTATGTCGGGGACATCACAGAATTCATTAGGGAACTCTATGGGTCAAGGGGTAGCATCAAATATGTTTGCTAATTCCCAAAGGCCAATGCCAGGAAGACAACAGGTAGTTCCCCAACAGCAACAGCAGCAACCCCAGAATTCACAGCAGTTTATGTACCAGCAACAGTTACAACAACAGCTAATGAAGCCAAATTTTAAGCCGGGAAACATCCAACTTGTGCAAAATCATATGCAACAGCAACAACAACAACAGCAGAACTTATTGCAACCATCTCAATTACAATCTTCTCAGCAATCTGTTATGCAACCTCCTGCAGTGCAGTCGTCTCTCTCTAGTCTTCAGCAGAATCAACAATCTGCTATGCAACACTCTTCACAATCCATGCTTCAGCAGCATCCTCAATCTGTTCTCCGGCAGCAACAGCAGCAGCCTCAACAGTCTTCTGTTGTCCATCAGCAGCAAACGCCAATCCCACAGCAGCCACTATTGCCCCCACAGCAGCAGCAACAACAGCAACAGCCACAGCTTATGGTGCAACAGCCAAATTCTACAAACTTGCCGCAGAATCAGCTTATTGGACAACAAAACAATGTAGGGGACATGCAGCAACAGCAACAGCAGAGGTTACTAAACCAGCAGAATAATATGTTAAACTTGCAACAGCAACAGCAACAGCAACAGCAACAACAACAACAACAACAACACCAATTAATGGCTCAGCAAAACAACCTCTCAAATATGCATCAGCAACAGTTGGGCCCTCAGAGTAATGTTACTGGATTACAGCAACAGCAGCAAACTGGTAACTCAAGCATGCAAACTAATCACCATCCTGCACACATGCTGCAATCCAAGGTTCAGCAACATCAGAGTTCATCATTGCCAACTCAAGGACATCAGTCCCAACCTCAGGCATCACAGCAACAATTATTATCACAGATTCAATCTCAGCCTGCACAGATGCAACAACAGATGAGTATGCAACAGCAATCAAACCCGCTACAACGAGATATGCAACAAAGGATCCAAGCATCAGGTCAGGTATCAGGATCTATGCTTCAACCACCAAATGTAATGGAACAGCAAAAGCAGTTATATCAGGCTCAAAGACCCCTTCCTGAGACGTCATCAACGTCTCTAGATTCCACAGCCCAGACTGCTGATTGGCAAGAGGAGGCCTATCAAAAGATCAAGGTCATGAAGGAGATGTACTACCCCGAACTTAGTGAAATGTACCAAAAAATTGCTGCTAAACTCCATCAGCATGACTCTCTTCCACAACAGCCAAAGTCAGAGCAGCTTGAGAAGCTGAAAATGTTTAAGGCCATGCTGGAGCGCCTCATAACAGTCTTACAAATTTCCAAAAGTAACATATCACCTGGAATGAAGGACAAGTTGGGTGTATACGAGAAGCAGATAGTAAATTTCATCAATACAAATAGACCCAGGAAGCCTGTTTCTACTCTGCAGCCTGGGCAGCTCCCTCCACCGCATATGCAGTCCATGCAGCAGTCACAATCCCAAATTACTCAAGTGCAATCTCATGAAAATCAAATGAACCCACAATTGCAATCCATGAACTTGCAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACGGGATTGCAGCAAAATTCTATGTCTTCTTTATCCGGGGTTTCAACAGCACAGCAGAACATGATGAATCCATTGCAGCCAAGTTCCAATATGGATCCAGGCCAGGGAACCTCACTGAACTCACTGCAGCAGGTCCCTGTGGGCTCTATCCAACAAACTCCTGTCAGTGCTCCCCAACAAGCTAACATGAATGCTTTATCATCACAGAATGGGGTTAACATGCTGCAGAACAACATTAATCCCCTTCAGTCAAATCCTGGTATTCTTCATAGCCAACATCTGAAACAGCAGGAACATCAATTGTATCATAATCAACTGAAGCAACAATATCAACAACGACAGATGCAGCAACAGTTGATGCAGAAGCAGCAGATAATTCAACAACAGCAGCAGCAACAACAACAGCTACAACAGCAAGCAAAGCAGCAGCTGCCTGGACAGTTGCAGGCTCACCAAATGCCACAGCTTCACCAAATCAGTGATGTTAATGATGTGAAAATTAGACCGGGGATGGGTGTTAAGCCAGGGTCTTTTCAGCAACATATGTCTGTAGGCCAGCGTGCATATCCTCATCAGCAATTGAAATCGGCATCCCCATATCCTGTTACATCAAGTAACCAACTCCTTCAGGCTCAATCTCCTCAACTTTCACAACATTCATCTCCACAAGTTGACCAGCAGTTGTTAGTCCCAAAAGCTGGGACCCCTTTGCAAAACGCTAGTTCACCATTTGTCATCCCATCTCCTCCAACTCCCATGGCTCCATCGCCTATGCCAGGGGAGTCAGAGAAACCTTCCTCGCTCTCAAATGCAGGGAATGTTGGACATCAACCAACAGCTGGTGTCGGAGCTCAGGTCCAGTCCCTTGCCATCGGTACCCCTGGGATATCAGCCTCTCCTTTGCTCGCTGAATTTATTGGTCCAGATGGAACTCATGCTAATGCTTTGTCAACTATATCTGGCAAGTCAAGTGTTACAGAGCAACCTCTTGAACGCTTGATTAAAGCGGTCAAGTCGATATCAACAAAGGCATTAAGTGCATCTGTCAGTGACATAGGCTCAGTAGTGAGTATGATTGATAGGATAGCAGGTTCAGCACCAGGTAATGGATCTAGAGCTGCAGTTGGTGAGGATCTGGTTGCTATGACAAAATGTCGTTTACAGGCAAGAAACTTCATCACTCATGATGGAACAACAAATGGGACAAGGAAAATGAGGCGATACACTAGCGCCATGCCCTTAAATGTTGTGTCATCAGGGGGCAGCATGAATGATAGTTTCAAGCAGTTGACTGGTTCGGAGACATCTGACCTGGAGTCAACTGCAACATCTCGTATCAAAAGGCCTAGGATTGAGGCTAATCATGCTCTTATGGAAGAAATAAGAGAAATAAATCGGCGGCTTATAGATACAGTTGTTGATATCAGTGATGAAGATGTTGATCCAAGTGCAGCTGCTGCTGAAAGGGGTGAAGGAACCATTGTCAAGTGCTCTTTTGATGCTGTGGCTCTCAGTCCCAACTTGAAATCGCAATATGCTTCAGCACAGATGTCTCCGATTCAGCCATTAAGACTGCTTGTTCCGACAAATTACCCAAACTGCTCTCCAATACTGTTAGACAAGTTTCCAGTTGAAGTCAGTAAGGAGTATGAAGATCTTTCTGTGAAGGCAAAATCAAGGTTTAGCATCTGTTTACGAAGCATTTCGCAGCCCATGTCTCTTGGGGAGATAGCAAGGACTTGGGATATTTGTGCACGTGCAGTTATTTCTGAGCATGCACAGCGAAGTGGTGGAGGCAGCTTCAGCTCAAAGTACGGGACATGGGAAAACTGCCTGAGTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1348

Amino Acids

148.78

Weight (kDa)

9.43

Isoelectric Point (pI)

71.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 98 4.1e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1703
Acc36I ACCTGC 2 cut(s) 2317, 3275
Acc65I GGTACC 1 cut(s) 3071
AccB1I GGYRCC 1 cut(s) 3071
AccB7I CCANNNNNTGG 4 cut(s) 1288, 2894, 3035, 3302
AccI GTMKAC 1 cut(s) 1980
AccII CGCG 1 cut(s) 88
AciI CCGC 9 cut(s) 80, 88, 314, 464, 1136, 1574, 2066, 3203, 3595
AcoI YGGCCR 1 cut(s) 145
AcuI CTGAAG 8 cut(s) 269, 899, 947, 2426, 2534, 2807, 3720, 3982
AcvI CACGTG 1 cut(s) 3953
AcyI GRCGYC 1 cut(s) 1700
AfaI GTAC 5 cut(s) 731, 1787, 1811, 3073, 4010
AhdI GACNNNNNGTC 2 cut(s) 3348, 3511
AjuI GAANNNNNNNTTGG 2 cut(s) 1955, 1987
AloI GAACNNNNNNTCC 2 cut(s) 3271, 3303
Alw21I GWGCWC 4 cut(s) 2368, 3049, 3689, 3952
Alw26I GTCTC 8 cut(s) 279, 527, 906, 1691, 1716, 3491, 3753, 3918
Alw44I GTGCAC 1 cut(s) 3948
AlwNI CAGNNNCTG 2 cut(s) 2626, 3653
ApaI GGGCCC 1 cut(s) 1337
ApaLI GTGCAC 1 cut(s) 3948
AseI ATTAAT 2 cut(s) 1286, 2433
Asp700I GAANNNNTTC 1 cut(s) 3898
Asp718I GGTACC 1 cut(s) 3071
AspA2I CCTAGG 1 cut(s) 3542
AspLEI GCGC 3 cut(s) 505, 1914, 3428
AspS9I GGNCC 7 cut(s) 330, 1333, 1334, 2329, 2898, 3052, 3116
AsuC2I CCSGG 4 cut(s) 30, 777, 2233, 2703
AsuHPI GGTGA 8 cut(s) 517, 1394, 1944, 2639, 2657, 2913, 3335, 3677
AvaII GGWCC 4 cut(s) 2329, 2898, 3052, 3116
AvrII CCTAGG 1 cut(s) 3542
AxyI CCTNAGG 1 cut(s) 1485
BaeGI GKGCMC 2 cut(s) 1337, 3952
BaeI ACNNNNGTAYC 2 cut(s) 3597, 3630
BamHI GGATCC 2 cut(s) 1599, 2290
BanI GGYRCC 1 cut(s) 3071
BanII GRGCYC 3 cut(s) 1337, 2342, 3049
BarI GAAGNNNNNNTAC 2 cut(s) 1769, 1801
BbrPI CACGTG 1 cut(s) 3953
BbsI GAAGAC 4 cut(s) 676, 905, 1008, 2214
Bbv12I GWGCWC 4 cut(s) 2368, 3049, 3689, 3952
BcgI CGANNNNNNTGC 2 cut(s) 879, 913
BciVI GTATCC 1 cut(s) 109
BcnI CCSGG 4 cut(s) 30, 777, 2233, 2703
BcoDI GTCTC 8 cut(s) 279, 527, 906, 1691, 1716, 3491, 3753, 3918
BfaI CTAG 6 cut(s) 909, 1715, 2916, 3309, 3423, 3543
BfmI CTRYAG 6 cut(s) 888, 2042, 2321, 2420, 2745, 3315
BfoI RGCGCY 2 cut(s) 1915, 3429
BfuAI ACCTGC 2 cut(s) 2317, 3275
BfuI GTATCC 1 cut(s) 109
BglII AGATCT 1 cut(s) 3850
BlnI CCTAGG 1 cut(s) 3542
BlpI GCTNAGC 1 cut(s) 1293
Bme18I GGWCC 4 cut(s) 2329, 2898, 3052, 3116
BmeRI GACNNNNNGTC 2 cut(s) 3348, 3511
BmgT120I GGNCC 7 cut(s) 330, 1333, 1334, 2329, 2898, 3052, 3116
BoxI GACNNNNGTC 1 cut(s) 1474
BpiI GAAGAC 4 cut(s) 676, 905, 1008, 2214
BplI GAGNNNNNCTC 4 cut(s) 10, 42, 259, 291
BpmI CTGGAG 3 cut(s) 492, 1928, 3530
Bpu10I CCTNAGC 1 cut(s) 3048
Bpu1102I GCTNAGC 1 cut(s) 1293
BpuEI CTTGAG 5 cut(s) 152, 1369, 1449, 1898, 2091
BpuMI CCSGG 4 cut(s) 30, 777, 2233, 2703
BsaAI YACGTR 1 cut(s) 3953
BsaBI GATNNNNATC 1 cut(s) 3636
BsaHI GRCGYC 1 cut(s) 1700
BsaI GGTCTC 1 cut(s) 527
BsaXI ACNNNNNCTCC 6 cut(s) 257, 287, 517, 547, 3036, 3066
Bse1I ACTGG 9 cut(s) 70, 220, 398, 475, 1357, 1381, 3055, 3493, 3824
Bse21I CCTNAGG 1 cut(s) 1485
Bse3DI GCAATG 3 cut(s) 51, 1454, 2267
Bse8I GATNNNNATC 1 cut(s) 3636
BseGI GGATG 7 cut(s) 55, 783, 970, 1405, 2712, 2786, 2931
BseJI GATNNNNATC 1 cut(s) 3636
BseMI GCAATG 3 cut(s) 51, 1454, 2267
BseNI ACTGG 9 cut(s) 70, 220, 398, 475, 1357, 1381, 3055, 3493, 3824
BseRI GAGGAG 6 cut(s) 15, 79, 206, 1763, 2826, 2931
BseSI GKGCMC 2 cut(s) 1337, 3952
BseYI CCCAGC 2 cut(s) 488, 2894
BsgI GTGCAG 5 cut(s) 914, 1395, 1515, 3666, 3975
Bsh1236I CGCG 1 cut(s) 88
BshNI GGYRCC 1 cut(s) 3071
BsiHKAI GWGCWC 4 cut(s) 2368, 3049, 3689, 3952
BsiSI CCGG 6 cut(s) 29, 144, 776, 988, 2232, 2702
BsmAI GTCTC 8 cut(s) 279, 527, 906, 1691, 1716, 3491, 3753, 3918
BsmBI CGTCTC 3 cut(s) 906, 1691, 1716
BsmI GAATGC 1 cut(s) 2392
Bso31I GGTCTC 1 cut(s) 527
Bsp120I GGGCCC 1 cut(s) 1333
Bsp1286I GDGCHC 6 cut(s) 1337, 2342, 2368, 3049, 3689, 3952
Bsp1720I GCTNAGC 1 cut(s) 1293
Bsp19I CCATGG 2 cut(s) 285, 2952
BspACI CCGC 9 cut(s) 80, 88, 314, 464, 1136, 1574, 2066, 3203, 3595
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 3 cut(s) 1773, 2119, 3379
BspMAI CTGCAG 5 cut(s) 892, 2046, 2325, 2424, 3319
BspMI ACCTGC 2 cut(s) 2317, 3275
BspT107I GGYRCC 1 cut(s) 3071
BspTNI GGTCTC 1 cut(s) 527
BsrDI GCAATG 3 cut(s) 51, 1454, 2267
BsrI ACTGG 9 cut(s) 70, 220, 398, 475, 1357, 1381, 3055, 3493, 3824
BssNAI GTATAC 1 cut(s) 1981
BssNI GRCGYC 1 cut(s) 1700
BssT1I CCWWGG 4 cut(s) 285, 1428, 2952, 3542
Bst1107I GTATAC 1 cut(s) 1981
Bst6I CTCTTC 3 cut(s) 119, 952, 1854
BstACI GRCGYC 1 cut(s) 1700
BstAPI GCANNNNNTGC 5 cut(s) 50, 2080, 2626, 3233, 3653
BstBAI YACGTR 1 cut(s) 3953
BstDSI CCRYGG 2 cut(s) 285, 2952
BstENI CCTNNNNNAGG 3 cut(s) 339, 516, 2970
BstF5I GGATG 7 cut(s) 55, 783, 970, 1405, 2712, 2786, 2931
BstFNI CGCG 1 cut(s) 88
BstH2I RGCGCY 2 cut(s) 1915, 3429
BstHHI GCGC 3 cut(s) 505, 1914, 3428
BstMAI GTCTC 8 cut(s) 279, 527, 906, 1691, 1716, 3491, 3753, 3918
BstNSI RCATGY 5 cut(s) 1180, 1393, 1420, 2419, 3974
BstPAI GACNNNNGTC 1 cut(s) 1474
BstSFI CTRYAG 6 cut(s) 888, 2042, 2321, 2420, 2745, 3315
BstSLI GKGCMC 2 cut(s) 1337, 3952
BstUI CGCG 1 cut(s) 88
BstV2I GAAGAC 4 cut(s) 676, 905, 1008, 2214
BstX2I RGATCY 7 cut(s) 344, 1599, 1625, 2290, 3305, 3328, 3850
BstYI RGATCY 7 cut(s) 344, 1599, 1625, 2290, 3305, 3328, 3850
BstZ17I GTATAC 1 cut(s) 1981
Bsu36I CCTNAGG 1 cut(s) 1485
BsuI GTATCC 1 cut(s) 109
BtgI CCRYGG 2 cut(s) 285, 2952
BtgZI GCGATG 1 cut(s) 2946
BtsCI GGATG 7 cut(s) 55, 783, 970, 1405, 2712, 2786, 2931
BtsI GCAGTG 2 cut(s) 897, 2318
BtsIMutI CAGTG 9 cut(s) 405, 468, 897, 2309, 2318, 2368, 2680, 3250, 3628
BveI ACCTGC 2 cut(s) 2317, 3275
CaiI CAGNNNCTG 2 cut(s) 2626, 3653
CciI TCATGA 3 cut(s) 1773, 2119, 3379
CfoI GCGC 3 cut(s) 505, 1914, 3428
Cfr13I GGNCC 7 cut(s) 330, 1333, 1334, 2329, 2898, 3052, 3116
CsiI ACCWGGT 1 cut(s) 3295
Csp6I GTAC 5 cut(s) 730, 1786, 1810, 3072, 4009
CviQI GTAC 5 cut(s) 730, 1786, 1810, 3072, 4009
DriI GACNNNNNGTC 2 cut(s) 3348, 3511
EaeI YGGCCR 1 cut(s) 145
Eam1104I CTCTTC 3 cut(s) 119, 952, 1854
Eam1105I GACNNNNNGTC 2 cut(s) 3348, 3511
EarI CTCTTC 3 cut(s) 119, 952, 1854
EciI GGCGGA 2 cut(s) 95, 453
Ecl136II GAGCTC 1 cut(s) 3047
Eco130I CCWWGG 4 cut(s) 285, 1428, 2952, 3542
Eco147I AGGCCT 3 cut(s) 22, 1754, 3541
Eco24I GRGCYC 3 cut(s) 1337, 2342, 3049
Eco31I GGTCTC 1 cut(s) 527
Eco32I GATATC 3 cut(s) 3084, 3216, 3619
Eco47I GGWCC 4 cut(s) 2329, 2898, 3052, 3116
Eco53kI GAGCTC 1 cut(s) 3047
Eco57I CTGAAG 8 cut(s) 269, 899, 947, 2426, 2534, 2807, 3720, 3982
Eco72I CACGTG 1 cut(s) 3953
Eco81I CCTNAGG 1 cut(s) 1485
EcoICRI GAGCTC 1 cut(s) 3047
EcoNI CCTNNNNNAGG 3 cut(s) 339, 516, 2970
EcoO109I RGGNCCY 4 cut(s) 330, 1334, 2329, 2898
EcoRI GAATTC 2 cut(s) 595, 712
EcoRV GATATC 3 cut(s) 3084, 3216, 3619
EcoT14I CCWWGG 4 cut(s) 285, 1428, 2952, 3542
EcoT22I ATGCAT 1 cut(s) 1320
EcoT38I GRGCYC 3 cut(s) 1337, 2342, 3049
ErhI CCWWGG 4 cut(s) 285, 1428, 2952, 3542
Esp3I CGTCTC 3 cut(s) 906, 1691, 1716
FauI CCCGC 2 cut(s) 307, 1581
FauNDI CATATG 3 cut(s) 804, 2070, 2739
FblI GTMKAC 1 cut(s) 1980
FokI GGATG 7 cut(s) 62, 770, 957, 1392, 2719, 2773, 2918
FriOI GRGCYC 3 cut(s) 1337, 2342, 3049
FspBI CTAG 6 cut(s) 909, 1715, 2916, 3309, 3423, 3543
GlaI GCGC 3 cut(s) 504, 1913, 3427
GsaI CCCAGC 2 cut(s) 492, 2898
GsuI CTGGAG 3 cut(s) 492, 1928, 3530
HaeII RGCGCY 2 cut(s) 1915, 3429
HapII CCGG 6 cut(s) 29, 144, 776, 988, 2232, 2702
HhaI GCGC 3 cut(s) 505, 1914, 3428
Hin1I GRCGYC 1 cut(s) 1700
Hin6I GCGC 3 cut(s) 503, 1912, 3426
HinP1I GCGC 3 cut(s) 503, 1912, 3426
HincII GTYRAC 5 cut(s) 2413, 2869, 3147, 3486, 3516
HindII GTYRAC 5 cut(s) 2413, 2869, 3147, 3486, 3516
HpaI GTTAAC 1 cut(s) 2413
HpaII CCGG 6 cut(s) 29, 144, 776, 988, 2232, 2702
HphI GGTGA 8 cut(s) 517, 1394, 1944, 2639, 2657, 2913, 3335, 3677
HpyAV CCTTC 8 cut(s) 1700, 1771, 1957, 2450, 2831, 3000, 3662, 3856
HpyCH4IV ACGT 3 cut(s) 1700, 1709, 3952
HpySE526I ACGT 3 cut(s) 1700, 1709, 3952
Hsp92I GRCGYC 1 cut(s) 1700
HspAI GCGC 3 cut(s) 503, 1912, 3426
KflI GGGWCCC 1 cut(s) 2898
KpnI GGTACC 1 cut(s) 3075
KspAI GTTAAC 1 cut(s) 2413
LmnI GCTCC 8 cut(s) 37, 66, 373, 1909, 2061, 2371, 2962, 3044
MabI ACCWGGT 1 cut(s) 3295
MaeI CTAG 6 cut(s) 909, 1715, 2916, 3309, 3423, 3543
MaeII ACGT 3 cut(s) 1700, 1709, 3952
MfeI CAATTG 3 cut(s) 2141, 2495, 2774
MflI RGATCY 7 cut(s) 344, 1599, 1625, 2290, 3305, 3328, 3850
MhlI GDGCHC 6 cut(s) 1337, 2342, 2368, 3049, 3689, 3952
MlyI GAGTC 4 cut(s) 526, 1838, 2987, 3521
MmeI TCCRAC 8 cut(s) 204, 532, 811, 2371, 2969, 2998, 3022, 3803
Mph1103I ATGCAT 1 cut(s) 1320
MroXI GAANNNNTTC 1 cut(s) 3898
MslI CAYNNNNRTG 2 cut(s) 1839, 3135
MspA1I CMGCKG 3 cut(s) 2626, 3035, 3650
MspI CCGG 6 cut(s) 29, 144, 776, 988, 2232, 2702
MunI CAATTG 3 cut(s) 2141, 2495, 2774
Mva1269I GAATGC 1 cut(s) 2392
MvnI CGCG 1 cut(s) 88
NciI CCSGG 4 cut(s) 30, 777, 2233, 2703
NcoI CCATGG 2 cut(s) 285, 2952
NdeI CATATG 3 cut(s) 804, 2070, 2739
NmuCI GTSAC 3 cut(s) 384, 2088, 3245
NsiI ATGCAT 1 cut(s) 1320
NspI RCATGY 5 cut(s) 1180, 1393, 1420, 2419, 3974
PaeI GCATGC 2 cut(s) 1393, 3974
PagI TCATGA 3 cut(s) 1773, 2119, 3379
PasI CCCWGGG 1 cut(s) 3077
PceI AGGCCT 3 cut(s) 22, 1754, 3541
PcsI WCGNNNNNNNCGW 1 cut(s) 1706
PctI GAATGC 1 cut(s) 2392
PdmI GAANNNNTTC 1 cut(s) 3898
PfeI GAWTC 9 cut(s) 83, 96, 373, 922, 1141, 1516, 1718, 2263, 3754
PflMI CCANNNNNTGG 4 cut(s) 1288, 2894, 3035, 3302
PfoI TCCNGGA 1 cut(s) 340
PleI GAGTC 4 cut(s) 526, 1838, 2986, 3520
PmaCI CACGTG 1 cut(s) 3953
PmlI CACGTG 1 cut(s) 3953
PpsI GAGTC 4 cut(s) 526, 1838, 2986, 3520
Ppu21I YACGTR 1 cut(s) 3953
PpuMI RGGWCCY 2 cut(s) 2329, 2898
PshAI GACNNNNGTC 1 cut(s) 1474
PshBI ATTAAT 2 cut(s) 1286, 2433
Psp124BI GAGCTC 1 cut(s) 3049
Psp5II RGGWCCY 2 cut(s) 2329, 2898
PspCI CACGTG 1 cut(s) 3953
PspFI CCCAGC 2 cut(s) 488, 2894
PspOMI GGGCCC 1 cut(s) 1333
PspPI GGNCC 7 cut(s) 330, 1333, 1334, 2329, 2898, 3052, 3116
PspPPI RGGWCCY 2 cut(s) 2329, 2898
PstI CTGCAG 5 cut(s) 892, 2046, 2325, 2424, 3319
PstNI CAGNNNCTG 2 cut(s) 2626, 3653
PsuI RGATCY 7 cut(s) 344, 1599, 1625, 2290, 3305, 3328, 3850
PvuII CAGCTG 3 cut(s) 2626, 3035, 3650
RsaI GTAC 5 cut(s) 731, 1787, 1811, 3073, 4010
RsaNI GTAC 5 cut(s) 730, 1786, 1810, 3072, 4009
RseI CAYNNNNRTG 2 cut(s) 1839, 3135
SacI GAGCTC 1 cut(s) 3049
Sau96I GGNCC 7 cut(s) 330, 1333, 1334, 2329, 2898, 3052, 3116
SchI GAGTC 4 cut(s) 526, 1838, 2987, 3521
SduI GDGCHC 6 cut(s) 1337, 2342, 2368, 3049, 3689, 3952
SexAI ACCWGGT 1 cut(s) 3295
SfcI CTRYAG 6 cut(s) 888, 2042, 2321, 2420, 2745, 3315
SinI GGWCC 4 cut(s) 2329, 2898, 3052, 3116
SmiMI CAYNNNNRTG 2 cut(s) 1839, 3135
SmlI CTYRAG 5 cut(s) 167, 1384, 1464, 1877, 2106
SmoI CTYRAG 5 cut(s) 167, 1384, 1464, 1877, 2106
SphI GCATGC 2 cut(s) 1393, 3974
SseBI AGGCCT 3 cut(s) 22, 1754, 3541
SsiI CCGC 9 cut(s) 80, 88, 314, 464, 1136, 1574, 2066, 3203, 3595
SspMI CTAG 6 cut(s) 909, 1715, 2916, 3309, 3423, 3543
SstI GAGCTC 1 cut(s) 3049
StuI AGGCCT 3 cut(s) 22, 1754, 3541
StyI CCWWGG 4 cut(s) 285, 1428, 2952, 3542
TaiI ACGT 3 cut(s) 1703, 1712, 3955
TaqI TCGA 1 cut(s) 3212
TatI WGTACW 1 cut(s) 1785
TauI GCSGC 3 cut(s) 91, 1138, 3598
TfiI GAWTC 9 cut(s) 83, 96, 373, 922, 1141, 1516, 1718, 2263, 3754
TscAI CASTG 9 cut(s) 405, 475, 897, 2316, 2325, 2368, 2680, 3250, 3628
TseFI GTSAC 3 cut(s) 384, 2088, 3245
Tsp45I GTSAC 3 cut(s) 384, 2088, 3245
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 9 cut(s) 405, 475, 897, 2316, 2325, 2368, 2680, 3250, 3628
Van91I CCANNNNNTGG 4 cut(s) 1288, 2894, 3035, 3302
VneI GTGCAC 1 cut(s) 3948
VpaK11BI GGWCC 4 cut(s) 2329, 2898, 3052, 3116
VspI ATTAAT 2 cut(s) 1286, 2433
XagI CCTNNNNNAGG 3 cut(s) 339, 516, 2970
XbaI TCTAGA 2 cut(s) 1714, 3308
XceI RCATGY 5 cut(s) 1180, 1393, 1420, 2419, 3974
XmaJI CCTAGG 1 cut(s) 3542
XmiI GTMKAC 1 cut(s) 1980
XmnI GAANNNNTTC 1 cut(s) 3898
XspI CTAG 6 cut(s) 909, 1715, 2916, 3309, 3423, 3543
ZraI GACGTC 1 cut(s) 1701
Zsp2I ATGCAT 1 cut(s) 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.