MD03G1032700.v1.1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006832
Physical Location & Seq
Reverse (-)
2634374 .. 2643269
8896 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1032700.v1.1.491

Sequence Viewer

Length: 4206 bp
ATGGATACCAATAAGTGGAGGCCTTCTCAAGTTGGAGAGGCTGCCATGGATGCCGGCGATTGGAGGAGTCAGCTGCAGGCTGATTCGAGGCAAAGAATTGTCAACAAGATAATGGATACGTTGAAGAGGCATCTTCCCTTCTCCGGACAAGATGGATTACAGGAACTCAGTAAAATTGCTGTAAGGTTTGAGGAAAAGATTTATACTGCAGCCACAAGCCAGTCGGATTATCTACGGAAAATTTCTCTGAAGATGCTCACAATGGAGACAAAGTCTCAGAATTCAATGGCCAATCCTTTACAATCTAACTCTGCGGGTAACAGCAACAGGCCCCCTGATCCGGGGTCTTTTGGTATGCAACCCCAAGTTCCCAATCAAGGTATGCAATCCCAAGTCCCCAATCATGGGCAATCACTCTCTATGCCGTTACCAGCTAATCAATCTCAAGGACGCCAACCACTTTTATCACAGAGCATTCAGAATAACATTCCACCTGCTGGAGTTCAAAGTTCGTCTGGCTTATCATCTGCACTACCTCCTTCTTCCGGTTTAACCCAGACTCCTATCCCAAGTATAGGTGGACAAAACCCAAACATGCAAAATATGTCTGGAAGTTCACAGAATTCATTAGGGAATTCCATGGGCCAGGGGGTACCCTCCAATGTCTTTGCTAATTCTCAAAGGCAAATGCCAGGAAGGCAACAGGTAGCTCCCCAACAGCAGCAGCCGCAGCAATCCCAGAATTCCCAGAATTCCCAGCAGTATATGTACCATCAACAGATGCAACATTCGCTTTTGAAGCCAAAATATCAGCAGGGAAATATGCCACAACTTGTGCAACAGCAACAGCAACAGCAGCAGAACCTTTTACAACCGACTCAGTTGCAATCTTCCCAGCAATCTGTTATGCCAACATCGTCTGTTATGCAGCCTTCAGTGCAATCATCTCTCCAGCAGAATCAACAATCTGCTATGCAACAGTCATCACAACCCATGCTTCCGCAGCATTCACAGCCAGTTCTCAGGCAGCAACAGCAGCCTCAACAGGTAGCTGTTCATCAACAGCAAACACCAATGGCACAACAGCCAATATTAACCCCGCAGCAGCAGCAGCCGCAGCTTATGGTGCAACAGCCAACTGCTTCAAACTTGTCCCAGAATCAGATGATTGGACAACAAACTAATGTCGGGGATATGCAGCAGCAGCAACAACAGAGGTTACTAAACCAGCAGAATAATGTTTTAAACCTGCAACAACAGCAACAGCAACATCAACAGCAGCAGCAGCAGCAACAGCAACAACAACAGCAGCAGCAGCAGTTAATGGCTCAGCAAAGCAACCTTTCTAATATCCATCAGCAACAATTGGGCCCGCAGAGTAATGTTACTGGGTTACAGCAACAGCAGCAAAGCAACCTTTCAAATATGCATCAGCAACAGTTGGGCTCTCAGAGTAATGTTACCGGATTACAGCAACAGCAGCACCTTGGAACTCAAAGTGGTAATTCTAGTATGCAACCAAATCAGCACTCTGTACACTTGTTGCAACAATCCAAGGTTCAGGTGCAGCAGCAACAACATCCGAATGCATCTAACATGTTACCGACTCAAGGACAGCAGTCACAACCGCAAGCATCACAGCAACAAATGATGTCACAGATTCAATCACAGCCTGCACAGATGCAACAAATGGGTTTGCAACAGCAGTCAAATCCACTGCAACGAGATATGCAGCAAAGGCTTCAAGCATCAGGTCAGATATCAGGAACCATGCTTCAAGCTCAAAATGTAATGGATCAGCAAAAGCAGTTATATCAATCTCAGAGACCTCTTGCTGAGACATCATCGACTTCTCTAGATTCCTCAGCTCAGACTGGACATGCAAATGGGGGTGATTGGCAAGAGGATGTCTATCAAAAAATCAAAGTCATGAAGGAAAAGTACTTACCCGAACTAAGTGAAATGTATCATAAAATTGCTACTAAACTCCAGCAGCACGATTCTCTTCCACAACAACCAAAGTCAGAGCAGCTTGAGAAGCTAAAAATGTTCAGAACCATGTTAGAGCGCCTCATATCAATTTTACAGGTTTCCAAGAGTAACATTTCACCTGGTTTGAAAGACAAGTTGGGTTTATATGAGAAGCAGATAGTAAATTTTATTAATACAAATAGACCGAGGAAGCAAGGTCCTCCTCTGCAACAAGGGCAGCTCCCCCCACCTCATATGCACTCCATGCAGCAGCCACAGTCTCAAATTCCTCAAGTGCAGTCTCATGAAAATCAAATGAACCCGCAGTTGCAAACAATGAATTTACAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACAAGTTTGCAGCAAAATTCAATGTCTTCAATATCTGGGCTTTCAACATCACAGCAGAACATGATGAATTCATTGCAGCCGAGTACAAATTTGGATTCAGGACAGGGAAATGCACTGAACTCTTTGCAGCAAGTTCCGGTGGGATCTATCCAACAAACTCCTGTCAGTGCTCCCCAGCAAGCTAACATGAATGGTTTGTCATCACAGAGCGGGGTTAGCATGCTTCAGCCAAATATTAACTCCCTTCAGTCAAATTCTGGTATGCTTCAACACCAGCAATTAAAACAGCAGGAACAGCAAATGCTTCAGAATCAACTGAAGCAACAGTATCAACAGCGAATGCAGCAGCAACTGATACAGAAGCAGCTGCAACAGCAACAGCAACAGCAGCAGCAGCAGCAACAGTTGCAGCAAGCAAAGCAGCAGCTTCCGGCGCAGATGCAGGCGAACCAACACCAAATGCCGCAACTTCATCAAATGAATGATGCAAATGACTTGAAGATGAGACAGGGGATGGGTGTTAAGCCAGGGGTTTTTCAGCAACATATATCCGCAGGCCAGCGTGCTTATCCGCATCAGCAGTTGAAATCAGGATCTCCATTTCCTATTCCATCATCAAACCAACTCCTTCAGGCTGCATCTCCTCAAATTTCACAACATTCTTCTCCCCAAGTTGACCAGCAGAATCTACTGACTCACCCAAAAGCTGGAACACCATTGCAAACTGCCGGTTCACCTTTTGTCATCCCATCTCCTTCAACTCCCATGGCTCCATCCCCCATGCCAGGGGATTCTGAGAAACCTTCCTCACTACCAAATGCTGGGAATATCGGACATCAACAAACAGCTGGGGTGGGAGCACCAGTCCAGTCCCTTGCAATTGGCACCCCTGGGATATCGGCATCTCCTTTGCTTGCTGAATTTAGTGTACCAGATGCTAGTCATGTTAATGCTTTGTCAACTATTTCTGGCAAGTCGAGTGTTACCGAGCAGCCTCTTGAACGCTTGATTAAGGCGGTGAAATCGATGTCACCTCATGCATTGAGTGCATCTGTTAGTGACATTGGTTCAGTTGTTAGTATGATTGATAGGATAGCAGGGTCTGCCCCAGGTAATGGATCTAGAGCGGCAGTCGGTGAGGATTTGGTTGCGATGACAAAGTGTCGTCTGCAAGCAAGAAACGTTATGACTCATGATGGATCAAATGGGACTAGGAAGATGAGGCGCTACACTAGTGCCATGCCCTTAAATGTTGTGTCTTCAGCTGGCAGTATGAATGATAGTTTCAAGCAGTTGATCAATTCAGAGACATCTGACCTGGAGTCAACTGCAACATCTCGTATTAAGAGGCCTAGGGTTGAGGCTAATCATGCTCTTCTGGAAGAAATAAGAGAAATAAATCGGCTGCTTATCGACACAGTTGTTGATATCAGTGATGAAGATGTTGACCCTAGTGCAGCCGCTGCTGAAGGGGGTGAGGGGACTCTTGTCAAGTGCTCTTTTGATGCTGTGGCTCTCAGTCCAAGCTTGAAATCACAGTATGCTTCAGCACAAATGTCACCAATTCAGCCATTAAGACTGCTTGTTCCCACAAATTACCCCAACTGCTCTCCAATACTCTTGGACAAGTTTCCAGTCGAAGTCAGTAAAGAGTATGAAGATCTTTCTGTGAAGGCCAAATCGATGTTTAGCATATCTCTACGAAGCATTTCACAACCCATGTCCCTTGGGGAGATAGCAAGGACTTGGGATGTTTGTGCACACGCAGTTATTTCTGAGCATGCGCAGCAGAGCGGTGGAGGAAGTTTCAGCTCAAAGTACGGGACATGGGAGAACTGCTTGAGCGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1402

Amino Acids

154.18

Weight (kDa)

9.28

Isoelectric Point (pI)

76.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 98 4.6e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 502
Acc16I TGCGCA 1 cut(s) 4137
Acc36I ACCTGC 2 cut(s) 502, 1257
Acc65I GGTACC 1 cut(s) 652
AccB1I GGYRCC 2 cut(s) 652, 3236
AccB7I CCANNNNNTGG 5 cut(s) 15, 497, 3059, 3173, 3467
AccBSI CCGCTC 3 cut(s) 2562, 3479, 4146
AccIII TCCGGA 1 cut(s) 143
AclI AACGTT 1 cut(s) 3534
AclWI GGATC 6 cut(s) 332, 1803, 2503, 2953, 3478, 3559
AcoI YGGCCR 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 451
AfaI GTAC 7 cut(s) 654, 770, 1536, 1943, 2437, 3282, 4172
AfeI AGCGCT 1 cut(s) 4198
AflIII ACRYGT 1 cut(s) 1596
AhdI GACNNNNNGTC 2 cut(s) 3513, 3673
AhlI ACTAGT 1 cut(s) 3584
AjnI CCWGG 8 cut(s) 645, 691, 2110, 2878, 3136, 3241, 3460, 3669
AjuI GAANNNNNNNTTGG 2 cut(s) 2111, 2143
Alw21I GWGCWC 4 cut(s) 2524, 3214, 3851, 4114
Alw26I GTCTC 8 cut(s) 260, 279, 1819, 1832, 2256, 2277, 2851, 3653
Alw44I GTGCAC 1 cut(s) 4110
AlwI GGATC 6 cut(s) 332, 1803, 2503, 2953, 3478, 3559
AlwNI CAGNNNCTG 3 cut(s) 2704, 3455, 3815
Aor13HI TCCGGA 1 cut(s) 143
Aor51HI AGCGCT 1 cut(s) 4198
AoxI GGCC 8 cut(s) 20, 288, 329, 643, 1369, 2908, 3701, 4026
ApaI GGGCCC 1 cut(s) 1373
ApaLI GTGCAC 1 cut(s) 4110
AseI ATTAAT 1 cut(s) 2163
Asp700I GAANNNNTTC 1 cut(s) 4060
Asp718I GGTACC 1 cut(s) 652
AspA2I CCTAGG 1 cut(s) 3704
AspLEI GCGC 5 cut(s) 2070, 2788, 3579, 4138, 4199
AspS9I GGNCC 5 cut(s) 330, 643, 1369, 1370, 2189
AsuC2I CCSGG 1 cut(s) 342
AsuHPI GGTGA 9 cut(s) 1904, 2100, 3041, 3078, 3375, 3382, 3500, 3839, 3903
AvaII GGWCC 1 cut(s) 2189
AvrII CCTAGG 1 cut(s) 3704
BaeGI GKGCMC 2 cut(s) 1373, 4114
BalI TGGCCA 1 cut(s) 290
BanI GGYRCC 2 cut(s) 652, 3236
BanII GRGCYC 2 cut(s) 1373, 1448
BarI GAAGNNNNNNTAC 2 cut(s) 1925, 1957
BbsI GAAGAC 2 cut(s) 2370, 3603
Bbv12I GWGCWC 4 cut(s) 2524, 3214, 3851, 4114
BbvCI CCTCAGC 1 cut(s) 1864
BccI CCATC 8 cut(s) 146, 780, 1362, 2860, 2971, 3109, 3133, 3542
BceAI ACGGC 1 cut(s) 409
BcgI CGANNNNNNTGC 2 cut(s) 865, 899
BciT130I CCWGG 8 cut(s) 647, 693, 2112, 2880, 3138, 3243, 3462, 3671
BciVI GTATCC 1 cut(s) 109
BclI TGATCA 1 cut(s) 3648
BcnI CCSGG 1 cut(s) 342
BcoDI GTCTC 8 cut(s) 260, 279, 1819, 1832, 2256, 2277, 2851, 3653
BcuI ACTAGT 1 cut(s) 3584
BfaI CTAG 8 cut(s) 1509, 1856, 3291, 3474, 3564, 3585, 3705, 3804
BfmI CTRYAG 2 cut(s) 74, 207
BfoI RGCGCY 3 cut(s) 2071, 3580, 4200
BfuAI ACCTGC 2 cut(s) 502, 1257
BfuI GTATCC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 697
BglII AGATCT 1 cut(s) 4012
BlnI CCTAGG 1 cut(s) 3704
BlpI GCTNAGC 1 cut(s) 1329
BmcAI AGTACT 1 cut(s) 1943
Bme1390I CCNGG 9 cut(s) 342, 647, 693, 2112, 2880, 3138, 3243, 3462, 3671
Bme18I GGWCC 1 cut(s) 2189
BmeRI GACNNNNNGTC 2 cut(s) 3513, 3673
BmgT120I GGNCC 5 cut(s) 330, 643, 1369, 1370, 2189
BmiI GGNNCC 6 cut(s) 332, 654, 1371, 1768, 3123, 3238
BmrFI CCNGG 9 cut(s) 342, 647, 693, 2112, 2880, 3138, 3243, 3462, 3671
BmrI ACTGGG 1 cut(s) 1398
BmuI ACTGGG 1 cut(s) 1398
BoxI GACNNNNGTC 2 cut(s) 1618, 3839
BpiI GAAGAC 2 cut(s) 2370, 3603
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 4 cut(s) 519, 935, 1973, 3692
Bpu10I CCTNAGC 1 cut(s) 1864
Bpu1102I GCTNAGC 1 cut(s) 1329
BpuEI CTTGAG 5 cut(s) 12, 429, 1593, 2054, 2247
BpuMI CCSGG 1 cut(s) 342
Bsa29I ATCGAT 2 cut(s) 3377, 4034
BsaBI GATNNNNATC 1 cut(s) 1911
BsaHI GRCGYC 1 cut(s) 451
BsaI GGTCTC 1 cut(s) 1819
BsaWI WCCGGW 4 cut(s) 143, 545, 1463, 2488
BsaXI ACNNNNNCTCC 6 cut(s) 257, 287, 544, 574, 3201, 3231
Bse118I RCCGGY 2 cut(s) 53, 3080
Bse1I ACTGG 7 cut(s) 220, 1016, 1393, 1879, 3215, 3220, 3986
Bse3DI GCAATG 2 cut(s) 2423, 3068
Bse8I GATNNNNATC 1 cut(s) 1911
BseAI TCCGGA 1 cut(s) 143
BseBI CCWGG 8 cut(s) 647, 693, 2112, 2880, 3138, 3243, 3462, 3671
BseCI ATCGAT 2 cut(s) 3377, 4034
BseGI GGATG 7 cut(s) 55, 1579, 1912, 2871, 3096, 3125, 4108
BseJI GATNNNNATC 1 cut(s) 1911
BseMI GCAATG 2 cut(s) 2423, 3068
BseNI ACTGG 7 cut(s) 220, 1016, 1393, 1879, 3215, 3220, 3986
BseRI GAGGAG 3 cut(s) 79, 2184, 2985
BseSI GKGCMC 2 cut(s) 1373, 4114
BseYI CCCAGC 5 cut(s) 756, 894, 2526, 3173, 3200
BsgI GTGCAG 5 cut(s) 513, 1586, 1659, 2288, 3828
BshFI GGCC 8 cut(s) 22, 290, 331, 645, 1371, 2910, 3703, 4028
BshNI GGYRCC 2 cut(s) 652, 3236
BshVI ATCGAT 2 cut(s) 3377, 4034
BsiHKAI GWGCWC 4 cut(s) 2524, 3214, 3851, 4114
BsiSI CCGG 8 cut(s) 54, 144, 341, 546, 1464, 2489, 2783, 3081
BslFI GGGAC 7 cut(s) 380, 1138, 3208, 3574, 3847, 4060, 4189
BsmAI GTCTC 8 cut(s) 260, 279, 1819, 1832, 2256, 2277, 2851, 3653
BsmFI GGGAC 7 cut(s) 380, 1138, 3208, 3574, 3847, 4060, 4189
BsmI GAATGC 4 cut(s) 474, 1006, 1591, 2697
BsnI GGCC 8 cut(s) 22, 290, 331, 645, 1371, 2910, 3703, 4028
Bso31I GGTCTC 1 cut(s) 1819
Bsp120I GGGCCC 1 cut(s) 1369
Bsp1286I GDGCHC 6 cut(s) 1373, 1448, 2524, 3214, 3851, 4114
Bsp13I TCCGGA 1 cut(s) 143
Bsp1407I TGTACA 1 cut(s) 1534
Bsp143I GATC 8 cut(s) 337, 1795, 2495, 2945, 3470, 3551, 3648, 4012
Bsp1720I GCTNAGC 1 cut(s) 1329
Bsp19I CCATGG 3 cut(s) 45, 639, 3117
BspANI GGCC 8 cut(s) 22, 290, 331, 645, 1371, 2910, 3703, 4028
BspDI ATCGAT 2 cut(s) 3377, 4034
BspEI TCCGGA 1 cut(s) 143
BspHI TCATGA 3 cut(s) 1929, 2275, 3544
BspLI GGNNCC 6 cut(s) 332, 654, 1371, 1768, 3123, 3238
BspMAI CTGCAG 2 cut(s) 78, 211
BspMI ACCTGC 2 cut(s) 502, 1257
BspPI GGATC 6 cut(s) 332, 1803, 2503, 2953, 3478, 3559
BspQI GCTCTTC 1 cut(s) 3732
BspT107I GGYRCC 2 cut(s) 652, 3236
BspTNI GGTCTC 1 cut(s) 1819
BsrBI CCGCTC 3 cut(s) 2562, 3479, 4146
BsrDI GCAATG 2 cut(s) 2423, 3068
BsrFI RCCGGY 2 cut(s) 53, 3080
BsrGI TGTACA 1 cut(s) 1534
BsrI ACTGG 7 cut(s) 220, 1016, 1393, 1879, 3215, 3220, 3986
BssAI RCCGGY 2 cut(s) 53, 3080
BssMI GATC 8 cut(s) 337, 1795, 2495, 2945, 3470, 3551, 3648, 4012
BssNI GRCGYC 1 cut(s) 451
BssT1I CCWWGG 7 cut(s) 45, 639, 1486, 1554, 3117, 3704, 4078
Bst2UI CCWGG 8 cut(s) 647, 693, 2112, 2880, 3138, 3243, 3462, 3671
Bst4CI ACNGT 7 cut(s) 981, 1440, 2250, 2679, 2757, 3772, 3891
Bst6I CTCTTC 3 cut(s) 119, 2010, 3732
BstACI GRCGYC 1 cut(s) 451
BstAPI GCANNNNNTGC 5 cut(s) 2236, 2758, 3398, 3455, 3815
BstAUI TGTACA 1 cut(s) 1534
BstDSI CCRYGG 3 cut(s) 45, 639, 3117
BstF5I GGATG 7 cut(s) 55, 1579, 1912, 2871, 3096, 3125, 4108
BstH2I RGCGCY 3 cut(s) 2071, 3580, 4200
BstHHI GCGC 5 cut(s) 2070, 2788, 3579, 4138, 4199
BstKTI GATC 8 cut(s) 340, 1798, 2498, 2948, 3473, 3554, 3651, 4015
BstMAI GTCTC 8 cut(s) 260, 279, 1819, 1832, 2256, 2277, 2851, 3653
BstMBI GATC 8 cut(s) 337, 1795, 2495, 2945, 3470, 3551, 3648, 4012
BstNI CCWGG 8 cut(s) 647, 693, 2112, 2880, 3138, 3243, 3462, 3671
BstNSI RCATGY 5 cut(s) 598, 1600, 1883, 2575, 4136
BstPAI GACNNNNGTC 2 cut(s) 1618, 3839
BstSCI CCNGG 9 cut(s) 340, 645, 691, 2110, 2878, 3136, 3241, 3460, 3669
BstSFI CTRYAG 2 cut(s) 74, 207
BstSLI GKGCMC 2 cut(s) 1373, 4114
BstV2I GAAGAC 2 cut(s) 2370, 3603
BstX2I RGATCY 4 cut(s) 2495, 2945, 3470, 4012
BstYI RGATCY 4 cut(s) 2495, 2945, 3470, 4012
Bsu15I ATCGAT 2 cut(s) 3377, 4034
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 8 cut(s) 22, 290, 331, 645, 1371, 2910, 3703, 4028
BsuTUI ATCGAT 2 cut(s) 3377, 4034
BtgI CCRYGG 3 cut(s) 45, 639, 3117
BtgZI GCGATG 1 cut(s) 3518
BtsCI GGATG 7 cut(s) 55, 1579, 1912, 2871, 3096, 3125, 4108
BtsI GCAGTG 1 cut(s) 1715
BtsIMutI CAGTG 5 cut(s) 942, 1715, 2465, 2524, 3790
BveI ACCTGC 2 cut(s) 502, 1257
CaiI CAGNNNCTG 3 cut(s) 2704, 3455, 3815
CciI TCATGA 3 cut(s) 1929, 2275, 3544
CfoI GCGC 5 cut(s) 2070, 2788, 3579, 4138, 4199
Cfr10I RCCGGY 2 cut(s) 53, 3080
Cfr13I GGNCC 5 cut(s) 330, 643, 1369, 1370, 2189
ClaI ATCGAT 2 cut(s) 3377, 4034
CseI GACGC 1 cut(s) 459
CsiI ACCWGGT 1 cut(s) 2110
Csp6I GTAC 7 cut(s) 653, 769, 1535, 1942, 2436, 3281, 4171
CviQI GTAC 7 cut(s) 653, 769, 1535, 1942, 2436, 3281, 4171
DpnI GATC 8 cut(s) 339, 1797, 2497, 2947, 3472, 3553, 3650, 4014
DpnII GATC 8 cut(s) 337, 1795, 2495, 2945, 3470, 3551, 3648, 4012
DraI TTTAAA 1 cut(s) 1245
DriI GACNNNNNGTC 2 cut(s) 3513, 3673
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 3 cut(s) 119, 2010, 3732
Eam1105I GACNNNNNGTC 2 cut(s) 3513, 3673
EarI CTCTTC 3 cut(s) 119, 2010, 3732
Eco130I CCWWGG 7 cut(s) 45, 639, 1486, 1554, 3117, 3704, 4078
Eco147I AGGCCT 2 cut(s) 22, 3703
Eco24I GRGCYC 2 cut(s) 1373, 1448
Eco31I GGTCTC 1 cut(s) 1819
Eco32I GATATC 3 cut(s) 1761, 3249, 3781
Eco47I GGWCC 1 cut(s) 2189
Eco47III AGCGCT 1 cut(s) 4198
EcoO109I RGGNCCY 2 cut(s) 330, 2189
EcoRI GAATTC 6 cut(s) 280, 622, 634, 742, 751, 2419
EcoRII CCWGG 8 cut(s) 645, 691, 2110, 2878, 3136, 3241, 3460, 3669
EcoRV GATATC 3 cut(s) 1761, 3249, 3781
EcoT14I CCWWGG 7 cut(s) 45, 639, 1486, 1554, 3117, 3704, 4078
EcoT22I ATGCAT 3 cut(s) 1431, 1591, 3394
EcoT38I GRGCYC 2 cut(s) 1373, 1448
ErhI CCWWGG 7 cut(s) 45, 639, 1486, 1554, 3117, 3704, 4078
FaqI GGGAC 7 cut(s) 380, 1138, 3208, 3574, 3847, 4060, 4189
FauI CCCGC 5 cut(s) 307, 1107, 1380, 2301, 2555
FauNDI CATATG 1 cut(s) 2226
FbaI TGATCA 1 cut(s) 3648
FokI GGATG 7 cut(s) 62, 1566, 1919, 2878, 3083, 3112, 4115
FriOI GRGCYC 2 cut(s) 1373, 1448
FspBI CTAG 8 cut(s) 1509, 1856, 3291, 3474, 3564, 3585, 3705, 3804
FspI TGCGCA 1 cut(s) 4137
GlaI GCGC 5 cut(s) 2069, 2787, 3578, 4137, 4198
GsaI CCCAGC 5 cut(s) 760, 898, 2530, 3177, 3204
GsuI CTGGAG 4 cut(s) 519, 935, 1973, 3692
HaeII RGCGCY 3 cut(s) 2071, 3580, 4200
HaeIII GGCC 8 cut(s) 22, 290, 331, 645, 1371, 2910, 3703, 4028
HapII CCGG 8 cut(s) 54, 144, 341, 546, 1464, 2489, 2783, 3081
HgaI GACGC 1 cut(s) 459
HhaI GCGC 5 cut(s) 2070, 2788, 3579, 4138, 4199
Hin1I GRCGYC 1 cut(s) 451
Hin6I GCGC 5 cut(s) 2068, 2786, 3577, 4136, 4197
HinP1I GCGC 5 cut(s) 2068, 2786, 3577, 4136, 4197
HincII GTYRAC 5 cut(s) 103, 3028, 3312, 3678, 3799
HindII GTYRAC 5 cut(s) 103, 3028, 3312, 3678, 3799
HindIII AAGCTT 1 cut(s) 3877
HpaII CCGG 8 cut(s) 54, 144, 341, 546, 1464, 2489, 2783, 3081
HphI GGTGA 9 cut(s) 1904, 2100, 3041, 3078, 3375, 3382, 3500, 3839, 3903
HpyCH4III ACNGT 7 cut(s) 981, 1440, 2250, 2679, 2757, 3772, 3891
HpyCH4IV ACGT 2 cut(s) 119, 3534
HpySE526I ACGT 2 cut(s) 119, 3534
Hsp92I GRCGYC 1 cut(s) 451
HspAI GCGC 5 cut(s) 2068, 2786, 3577, 4136, 4197
Kpn2I TCCGGA 1 cut(s) 143
KpnI GGTACC 1 cut(s) 656
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Ksp22I TGATCA 1 cut(s) 3648
Kzo9I GATC 8 cut(s) 337, 1795, 2495, 2945, 3470, 3551, 3648, 4012
LguI GCTCTTC 1 cut(s) 3732
LmnI GCTCC 5 cut(s) 715, 2217, 2527, 3127, 3209
MabI ACCWGGT 1 cut(s) 2110
MaeI CTAG 8 cut(s) 1509, 1856, 3291, 3474, 3564, 3585, 3705, 3804
MaeII ACGT 2 cut(s) 119, 3534
MalI GATC 8 cut(s) 339, 1797, 2497, 2947, 3472, 3553, 3650, 4014
MbiI CCGCTC 3 cut(s) 2562, 3479, 4146
MboI GATC 8 cut(s) 337, 1795, 2495, 2945, 3470, 3551, 3648, 4012
MfeI CAATTG 2 cut(s) 1364, 3231
MflI RGATCY 4 cut(s) 2495, 2945, 3470, 4012
MhlI GDGCHC 6 cut(s) 1373, 1448, 2524, 3214, 3851, 4114
MlsI TGGCCA 1 cut(s) 290
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 8 cut(s) 76, 553, 871, 1600, 3040, 3535, 3683, 3829
MmeI TCCRAC 3 cut(s) 13, 204, 2527
Mox20I TGGCCA 1 cut(s) 290
Mph1103I ATGCAT 3 cut(s) 1431, 1591, 3394
MroI TCCGGA 1 cut(s) 143
MroNI GCCGGC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 4060
MscI TGGCCA 1 cut(s) 290
MslI CAYNNNNRTG 3 cut(s) 1584, 1884, 3300
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 5 cut(s) 73, 2719, 3200, 3617, 3815
MspI CCGG 8 cut(s) 54, 144, 341, 546, 1464, 2489, 2783, 3081
MspR9I CCNGG 9 cut(s) 342, 647, 693, 2112, 2880, 3138, 3243, 3462, 3671
MunI CAATTG 2 cut(s) 1364, 3231
Mva1269I GAATGC 4 cut(s) 474, 1006, 1591, 2697
MvaI CCWGG 8 cut(s) 647, 693, 2112, 2880, 3138, 3243, 3462, 3671
NaeI GCCGGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 342
NcoI CCATGG 3 cut(s) 45, 639, 3117
NdeI CATATG 1 cut(s) 2226
NdeII GATC 8 cut(s) 337, 1795, 2495, 2945, 3470, 3551, 3648, 4012
NgoMIV GCCGGC 1 cut(s) 53
NlaIV GGNNCC 6 cut(s) 332, 654, 1371, 1768, 3123, 3238
NmeAIII GCCGAG 1 cut(s) 2457
NmuCI GTSAC 5 cut(s) 1620, 1653, 3381, 3410, 3909
NsbI TGCGCA 1 cut(s) 4137
NsiI ATGCAT 3 cut(s) 1431, 1591, 3394
NspI RCATGY 5 cut(s) 598, 1600, 1883, 2575, 4136
PaeI GCATGC 2 cut(s) 2575, 4136
PagI TCATGA 3 cut(s) 1929, 2275, 3544
PaqCI CACCTGC 1 cut(s) 502
PasI CCCWGGG 1 cut(s) 3242
PceI AGGCCT 2 cut(s) 22, 3703
PciI ACATGT 1 cut(s) 1596
PciSI GCTCTTC 1 cut(s) 3732
PctI GAATGC 4 cut(s) 474, 1006, 1591, 2697
PdiI GCCGGC 1 cut(s) 55
PdmI GAANNNNTTC 1 cut(s) 4060
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 5 cut(s) 15, 497, 3059, 3173, 3467
PleI GAGTC 8 cut(s) 75, 553, 871, 1600, 3040, 3535, 3682, 3829
PpsI GAGTC 8 cut(s) 75, 553, 871, 1600, 3040, 3535, 3682, 3829
PpuMI RGGWCCY 1 cut(s) 2189
PscI ACATGT 1 cut(s) 1596
PshAI GACNNNNGTC 2 cut(s) 1618, 3839
PshBI ATTAAT 1 cut(s) 2163
Psp1406I AACGTT 1 cut(s) 3534
Psp5II RGGWCCY 1 cut(s) 2189
Psp6I CCWGG 8 cut(s) 645, 691, 2110, 2878, 3136, 3241, 3460, 3669
PspFI CCCAGC 5 cut(s) 756, 894, 2526, 3173, 3200
PspGI CCWGG 8 cut(s) 645, 691, 2110, 2878, 3136, 3241, 3460, 3669
PspN4I GGNNCC 6 cut(s) 332, 654, 1371, 1768, 3123, 3238
PspOMI GGGCCC 1 cut(s) 1369
PspPI GGNCC 5 cut(s) 330, 643, 1369, 1370, 2189
PspPPI RGGWCCY 1 cut(s) 2189
PstI CTGCAG 2 cut(s) 78, 211
PstNI CAGNNNCTG 3 cut(s) 2704, 3455, 3815
PsuI RGATCY 4 cut(s) 2495, 2945, 3470, 4012
PsyI GACNNNGTC 1 cut(s) 271
PvuII CAGCTG 4 cut(s) 73, 2719, 3200, 3617
RsaI GTAC 7 cut(s) 654, 770, 1536, 1943, 2437, 3282, 4172
RsaNI GTAC 7 cut(s) 653, 769, 1535, 1942, 2436, 3281, 4171
RseI CAYNNNNRTG 3 cut(s) 1584, 1884, 3300
SapI GCTCTTC 1 cut(s) 3732
Sau3AI GATC 8 cut(s) 337, 1795, 2495, 2945, 3470, 3551, 3648, 4012
Sau96I GGNCC 5 cut(s) 330, 643, 1369, 1370, 2189
ScaI AGTACT 1 cut(s) 1943
SchI GAGTC 8 cut(s) 76, 553, 871, 1600, 3040, 3535, 3683, 3829
ScrFI CCNGG 9 cut(s) 342, 647, 693, 2112, 2880, 3138, 3243, 3462, 3671
SduI GDGCHC 6 cut(s) 1373, 1448, 2524, 3214, 3851, 4114
SexAI ACCWGGT 1 cut(s) 2110
SfcI CTRYAG 2 cut(s) 74, 207
SinI GGWCC 1 cut(s) 2189
SmiMI CAYNNNNRTG 3 cut(s) 1584, 1884, 3300
SmlI CTYRAG 6 cut(s) 27, 444, 1608, 2033, 2262, 4192
SmoI CTYRAG 6 cut(s) 27, 444, 1608, 2033, 2262, 4192
SpeI ACTAGT 1 cut(s) 3584
SphI GCATGC 2 cut(s) 2575, 4136
SseBI AGGCCT 2 cut(s) 22, 3703
SspI AATATT 2 cut(s) 1092, 2587
SspMI CTAG 8 cut(s) 1509, 1856, 3291, 3474, 3564, 3585, 3705, 3804
StuI AGGCCT 2 cut(s) 22, 3703
StyD4I CCNGG 9 cut(s) 340, 645, 691, 2110, 2878, 3136, 3241, 3460, 3669
StyI CCWWGG 7 cut(s) 45, 639, 1486, 1554, 3117, 3704, 4078
TaaI ACNGT 7 cut(s) 981, 1440, 2250, 2679, 2757, 3772, 3891
TaiI ACGT 2 cut(s) 122, 3537
TaqI TCGA 7 cut(s) 86, 1847, 3329, 3377, 3765, 3990, 4034
TaqII GACCGA 1 cut(s) 2191
TatI WGTACW 3 cut(s) 1534, 1941, 2435
TauI GCSGC 5 cut(s) 730, 1117, 2818, 3482, 3815
TscAI CASTG 5 cut(s) 942, 1722, 2472, 2524, 3790
TseFI GTSAC 5 cut(s) 1620, 1653, 3381, 3410, 3909
Tsp45I GTSAC 5 cut(s) 1620, 1653, 3381, 3410, 3909
TspGWI ACGGA 1 cut(s) 250
TspRI CASTG 5 cut(s) 942, 1722, 2472, 2524, 3790
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 5 cut(s) 15, 497, 3059, 3173, 3467
VneI GTGCAC 1 cut(s) 4110
VpaK11BI GGWCC 1 cut(s) 2189
VspI ATTAAT 1 cut(s) 2163
XbaI TCTAGA 2 cut(s) 1855, 3473
XceI RCATGY 5 cut(s) 598, 1600, 1883, 2575, 4136
XmaJI CCTAGG 1 cut(s) 3704
XmnI GAANNNNTTC 1 cut(s) 4060
XspI CTAG 8 cut(s) 1509, 1856, 3291, 3474, 3564, 3585, 3705, 3804
ZrmI AGTACT 1 cut(s) 1943
Zsp2I ATGCAT 3 cut(s) 1431, 1591, 3394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.