Rw5G046410

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006832
Physical Location & Seq
Forward (+)
80572041 .. 80578585
6545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G046410.1

Sequence Viewer

Length: 4050 bp
ATGGACACGAATAATTGGAGGCCTCCTCAAGGCGCAGAGCCTGCAATGGATGCAGGAGATTGGAGGAGCCAATTACAGCAGGATTCGCGGCAGAGAATCGTCAATAAGATAATGGATACATTGAAGAGGCATCTTCCCTTCTCCGGTCAAGAGGGATTGCACGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATACTGCTGCTACAAGCCAGTCGGATTATCTACGGAAAATTTCTCTGAAAATGCTCACTATGGAGACCAAGTCTCAGAACACAATTGGCACTTCTTTACCAAGCAACTCTGCTGGTAATAGTAACAGGCCCCCTGATCCAGGATCTTTCATGCAACCCCAAGTTCCCAATCAAGGACAGTCACTCCCTATGCCAGTGCCAGCCAATCAATCTCAACCACGCCAACAACTCTTATCACAGAACATTCAGAATAACATTCCCCCCAATTCTGCTGGCTTATCATCTGCATTATCTCCTGCATCTGGGCTCACCCAGACTCCTGTACCGAGTATTGTTGGAAATGCAAACTTGCAAAGTATGTCGGGGACATCACAGAATTCATTGGGGAACTCCATCGGCCAGGGGGTAACTTCAAATATGTTTGCTAATTCCCAAAGGCAAATGCCAGGACGACAACAGGTAGTTCCGCAGCAGCAACAGCAGCAAACCCAGAATTCACAGCAGTATATGTACCAGCAACAGTTACAACAACAGCTCATGAAGCCAAAATTTCAGCAGCAGGGAAACATCCAACTTGTGCAAAATCATATGCAACAGCAACAACAGCAGCAGCAGAACTTATTGCAATCACCTCAGTTGCAATCTTCTCAGCAATCTGTTATGCAACCTCCTGCAGTGCAGTCGTCTCTCTCTAGTCTTCAGCAGAATCAACAATCTGCTATGCAACACTCTTCACAAGCCATGCTTCAGCAGCATTCTCAATCTGTTCTGCGGCAGCAACAGCAGCCTCAACAGTCTTCTGTTGTTCATCAGCAGCAAACACCAATCCCACAGCAGTCAATACTGCCCCCACAGCAGCAGCAGCAACAGCCACAGCTTATGGTGCAACAGCCAAATTCAACAAACTTGCCGCAGAATCAGCTTATTGGACAACAAAACAATGTAGGGGACATGCAGCAACAGCAACAGCAACAGCAACAGCAACAGCAAAGGTTACTAAGCCAGCAGAATAATATGTTAAACTTGCAACAGCAGCAGCAGCAACAACAACACCAATTAATGGCTCAGCAAAACAACCTCTCAAATATGCATCAGCAACAGTTGGGCACTCAGAGTAATGTTACTGGATTACAGCAACAGCAGCACCTTGGTACTCAAACTGGTAACTCAAGCATGCAAACTAATCAGCACTCTGCACACATGCTGCAATCCAAGGTTCAGCAACAACATCAGAGTCCAGCATTACCGATTCAAGGACATCAGTCCCAACCTCAGGTGTCACAGCAACAATTATTATCACAGATTCAATCTCAGCCTGCACAGATACAACAACAGATGGGTATGCAACAGCAATCAAACCAGCTACAACGAGATCTGCAGCAAAGGATCCAAGCATCAGGTCAGGTATCAGGATCCATGCTTCAACCACCAAATGTAATGGATCCGCAAAAGCAGTTATATCAAGCTCAAAGAGCCCTTCCTGAGACGTCATCAACGTCTCTAGATTCCACAGCCCAGACTGGACATACAAATGGTGCTGATTGGCAAGAGGAGGTCTATCAAAAGATCAAAGTCATGAAGGATATGTACTACCCCGAACTAAGTGAAATGTACCAAAAAATTGCTACTAAACTTCATCAGCACGACTCCCTTCCACAACAGCCAAAGTCAGATCAGCTTGAGAAGCTGAAGATGTTTAAGACCATGCTGGAGCGCCTGATAACAGTCTTACAAATTTCCAAAACTAGCATAACACCTGGTTTGAAGGAGAAGTTGGGCTTATACGAGAAGCAGATAATAAATTTTATTAATACAAATAGACCCAGGAAGCCTGTTTCTTCTCTGCCGCCAGGGCAGCTCCCCCCACCGCATATGCAGTCCATGCAGCAGTCACAATCCCAGATTACTCAAGTGCAATCTCATGAAAATCAGATGAACCCACAATTGCAATCCCTGAACTTGCAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACGGGCTTGCAGCAAAATTCTATGTCTTCTTTATCTGGGGTTTCAACAGCACAGCAAAACATGATGAATCCGTTGCAGCCAAGTTCCAATATGGATCCAGGACAAGGAACCGCACTGAACTCACTGCAGCAGGTCCCTGTGGGCTCTATCCAACAGACTCCTGTCAGTGCTTCCCAACAAGCTAACATGAATGCCTTATCATCACAGAATGGGATTAACATGCTGCAGAGCAACATTAATCCCCTTCAGTCAAATTCTGGTATGCTTCATAGCCAACATCTGAAACAGCAGGAACAGCAATTGTATCAGAATCAACTCAAGCAACAACAATTTCAACAACGACAGATGCAGCAGCAGCAGTTGATGCAGAAGCAGCTAATTCAACAACAGCAGCAGCAGCAACAGCAACAGCAACAACAGCTACAACAGCAAGCAAAGCAGCAGCTGCCTGGACAGTTGCAGGCACACCAAATGCCACAGCTTCACCAAATCAGTGATGTTAATGATGTGAAAGTAAGGCCGGGGATGGGTGTTAAGTCAGGGGCCTTTCAGCAACAAATGTCTGTAGGCCAGCGTGCTTATCCTCATTCGCAATTAAAACCGGGATCTCCATATCCTATCACATCAAGCAACCAGCTCCTTCAGGCTCAATCTCCTCAACTGTCACAGCATTCTTCTCCACAAGTTGACCAGCCGATGTTAGTCCCAAAAGCTCAAAATGCTAGCTCACCATTTGTCATCCCATCTCCTTCAACTCCCATTGCGCCATCACCTATGCCAGGAGATTCAGAGAAACCTTCCTCACTCGCAAATGCTGGGAATGTTGGACATCAACAAGCAACTGTTGTCGGAGCTCAGGTCCAGTCCCTTGCCATCGGCACCCCTGGGATATCAGCCTCTCCTTTGCTTGCTGAATTTATCGGTCCAGATGGCACTCATGCTAATGCTTTGTCAGCAATATCTGGCAAGTCAAGTGTTACAGAGCAACCTCTTGAACGCTTGATTAAAGCGGTCAAGTCAATATCACCAAAGGCATTACGTGCATCTGTCAGTGACATAGGCTCAGTGGTGAGTATGATTGATAGGATAGCAGGTTCAGCACCAGGTAATGGATCTAGAGCTGCAGTTGGTGAGGATCTGGTTGCTATGACAAAATGTCGTCTACAGGCTAGAAACTTCATCACTCATGATGGAACAACAAATGGGACAAGGAAAATGAGGCGATACACTAGTGCCATGCCCTTAAATGTTGTGTCATCAGGTGGCAGCATGAATGATAGTTTCAAGCAGTTGACCTGTTCAGAGACATCTGACCTGGAGTCAACTGCAACGTCTCGTATCAAAAGGCCTAGGATTGAGGCTAATCATGCTCTTATGGAAGAAATAAGAGAAATAAATCGGCGGCTTATAGATACAGTTGTTGATATCAGTGATGAAGATGTTGATCCAAGTGCGGCTGCTGCTGAAGGGGGTGAAGGAACCATCGTCAAGTGCTCTTTTGATGCTGTGGCTCTCAGTCCCAACTTGAAATCGCAGTATGCTTCAGCACAAATGTCTCCGATTCAGCCATTAAGACTGCTTGTTCCGACAAATTATCCCAACTGCTCTCCAATACTGTTAGACAAGTTTCCGGTTGAAGTCAGTAAGGAGTATGAAGATCTTTCTGTGAAGGCAAAATCAAGGTTTAGCATCTCTTTACGAAGCATTTCGCAGCCCATGTCCCTTGGGGAGATAGCAAGGGCTTGGGATAATTGTGCACGTACAGTTATTTCTGAGCATGCACAGCGAAGTGGGGGAGGCAGCTTCAGCTCAAAGTATGGGACATGGGAGAACTGCCTGAGTGCCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1349

Amino Acids

148.97

Weight (kDa)

9.43

Isoelectric Point (pI)

71.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 97 2.1e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1691
Acc36I ACCTGC 2 cut(s) 2320, 3281
AccB1I GGYRCC 1 cut(s) 3077
AccB7I CCANNNNNTGG 2 cut(s) 1261, 3308
AccI GTMKAC 1 cut(s) 3361
AccII CGCG 1 cut(s) 88
AcoI YGGCCR 1 cut(s) 598
AcuI CTGAAG 8 cut(s) 884, 932, 1910, 2429, 2825, 3684, 3726, 3988
AcyI GRCGYC 1 cut(s) 1688
AfaI GTAC 6 cut(s) 525, 713, 1354, 1790, 1814, 3961
AhdI GACNNNNNGTC 2 cut(s) 3354, 3517
AhlI ACTAGT 1 cut(s) 3428
AjuI GAANNNNNNNTTGG 2 cut(s) 1958, 1990
AloI GAACNNNNNNTCC 2 cut(s) 3277, 3309
Alw21I GWGCWC 3 cut(s) 3055, 3695, 3958
Alw26I GTCTC 8 cut(s) 260, 279, 891, 1679, 1704, 3497, 3537, 3759
Alw44I GTGCAC 1 cut(s) 3954
AlwNI CAGNNNCTG 2 cut(s) 41, 2644
AoxI GGCC 7 cut(s) 20, 329, 598, 2717, 2742, 2767, 3545
ApaLI GTGCAC 1 cut(s) 3954
AseI ATTAAT 3 cut(s) 1259, 2010, 2436
Asp700I GAANNNNTTC 1 cut(s) 3904
AspA2I CCTAGG 1 cut(s) 3548
AspLEI GCGC 3 cut(s) 35, 1917, 2965
AspS9I GGNCC 5 cut(s) 330, 2332, 2742, 3058, 3122
AsuC2I CCSGG 2 cut(s) 2721, 2802
AsuHPI GGTGA 9 cut(s) 502, 822, 2675, 2919, 2961, 3216, 3280, 3341, 3683
AsuNHI GCTAGC 1 cut(s) 2921
AvaII GGWCC 3 cut(s) 2332, 3058, 3122
AvrII CCTAGG 1 cut(s) 3548
AxyI CCTNAGG 1 cut(s) 1473
BaeGI GKGCMC 2 cut(s) 1310, 3958
BaeI ACNNNNGTAYC 6 cut(s) 507, 540, 3415, 3448, 3603, 3636
BamHI GGATCC 4 cut(s) 1587, 1613, 1642, 2293
BanI GGYRCC 1 cut(s) 3077
BanII GRGCYC 4 cut(s) 510, 1678, 2345, 3055
BarI GAAGNNNNNNTAC 2 cut(s) 1772, 1804
BbsI GAAGAC 3 cut(s) 890, 990, 2217
Bbv12I GWGCWC 3 cut(s) 3055, 3695, 3958
BccI CCATC 9 cut(s) 602, 1531, 2719, 2950, 2974, 3080, 3122, 3383, 3689
BcgI CGANNNNNNTGC 2 cut(s) 864, 898
BciVI GTATCC 1 cut(s) 109
BcnI CCSGG 2 cut(s) 2721, 2802
BcoDI GTCTC 8 cut(s) 260, 279, 891, 1679, 1704, 3497, 3537, 3759
BcuI ACTAGT 1 cut(s) 3428
BfaI CTAG 8 cut(s) 894, 1703, 1947, 2922, 3315, 3369, 3429, 3549
BfmI CTRYAG 7 cut(s) 873, 1577, 2324, 2423, 2763, 3321, 3362
BfoI RGCGCY 1 cut(s) 1918
BfuAI ACCTGC 2 cut(s) 2320, 3281
BfuI GTATCC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 2053
BglII AGATCT 2 cut(s) 1573, 3856
BlnI CCTAGG 1 cut(s) 3548
BlpI GCTNAGC 1 cut(s) 1266
Bme18I GGWCC 3 cut(s) 2332, 3058, 3122
BmeRI GACNNNNNGTC 2 cut(s) 3354, 3517
BmgT120I GGNCC 5 cut(s) 330, 2332, 2742, 3058, 3122
BmtI GCTAGC 1 cut(s) 2925
BoxI GACNNNNGTC 2 cut(s) 1462, 2360
BpiI GAAGAC 3 cut(s) 890, 990, 2217
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 2 cut(s) 1931, 3536
Bpu10I CCTNAGC 1 cut(s) 3054
Bpu1102I GCTNAGC 1 cut(s) 1266
BpuEI CTTGAG 6 cut(s) 12, 152, 1354, 1901, 2094, 2501
BpuMI CCSGG 2 cut(s) 2721, 2802
BsaAI YACGTR 2 cut(s) 3239, 3959
BsaBI GATNNNNATC 1 cut(s) 3642
BsaHI GRCGYC 1 cut(s) 1688
BsaI GGTCTC 1 cut(s) 260
BsaWI WCCGGW 2 cut(s) 143, 3829
BsaXI ACNNNNNCTCC 8 cut(s) 257, 287, 369, 399, 502, 532, 3042, 3072
Bse1I ACTGG 6 cut(s) 220, 395, 1330, 1366, 1726, 3061
Bse21I CCTNAGG 1 cut(s) 1473
Bse3DI GCAATG 2 cut(s) 51, 2958
Bse8I GATNNNNATC 1 cut(s) 3642
BseGI GGATG 4 cut(s) 55, 768, 2730, 2937
BseJI GATNNNNATC 1 cut(s) 3642
BseMI GCAATG 2 cut(s) 51, 2958
BseNI ACTGG 6 cut(s) 220, 395, 1330, 1366, 1726, 3061
BseRI GAGGAG 4 cut(s) 15, 79, 1766, 2844
BseSI GKGCMC 2 cut(s) 1310, 3958
BseYI CCCAGC 1 cut(s) 3014
BsgI GTGCAG 3 cut(s) 899, 1380, 1503
Bsh1236I CGCG 1 cut(s) 88
BshFI GGCC 7 cut(s) 22, 331, 600, 2719, 2744, 2769, 3547
BshNI GGYRCC 1 cut(s) 3077
BsiHKAI GWGCWC 3 cut(s) 3055, 3695, 3958
BsiSI CCGG 4 cut(s) 144, 2720, 2801, 3830
BsmAI GTCTC 8 cut(s) 260, 279, 891, 1679, 1704, 3497, 3537, 3759
BsmBI CGTCTC 4 cut(s) 891, 1679, 1704, 3537
BsmI GAATGC 3 cut(s) 955, 2395, 2869
BsnI GGCC 7 cut(s) 22, 331, 600, 2719, 2744, 2769, 3547
Bso31I GGTCTC 1 cut(s) 260
Bsp1286I GDGCHC 7 cut(s) 510, 1310, 1678, 2345, 3055, 3695, 3958
Bsp1720I GCTNAGC 1 cut(s) 1266
BspANI GGCC 7 cut(s) 22, 331, 600, 2719, 2744, 2769, 3547
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 4 cut(s) 738, 1776, 2122, 3385
BspMAI CTGCAG 5 cut(s) 877, 1581, 2328, 2427, 3325
BspMI ACCTGC 2 cut(s) 2320, 3281
BspOI GCTAGC 1 cut(s) 2925
BspT107I GGYRCC 1 cut(s) 3077
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 2 cut(s) 51, 2958
BsrI ACTGG 6 cut(s) 220, 395, 1330, 1366, 1726, 3061
BssNI GRCGYC 1 cut(s) 1688
BssT1I CCWWGG 4 cut(s) 1348, 1413, 3548, 3922
Bst6I CTCTTC 2 cut(s) 119, 937
BstACI GRCGYC 1 cut(s) 1688
BstAPI GCANNNNNTGC 6 cut(s) 41, 50, 2083, 2563, 2644, 3239
BstBAI YACGTR 2 cut(s) 3239, 3959
BstENI CCTNNNNNAGG 3 cut(s) 27, 339, 2976
BstF5I GGATG 4 cut(s) 55, 768, 2730, 2937
BstFNI CGCG 1 cut(s) 88
BstH2I RGCGCY 1 cut(s) 1918
BstHHI GCGC 3 cut(s) 35, 1917, 2965
BstMAI GTCTC 8 cut(s) 260, 279, 891, 1679, 1704, 3497, 3537, 3759
BstNSI RCATGY 5 cut(s) 1156, 1378, 1405, 2422, 3980
BstPAI GACNNNNGTC 2 cut(s) 1462, 2360
BstSFI CTRYAG 7 cut(s) 873, 1577, 2324, 2423, 2763, 3321, 3362
BstSLI GKGCMC 2 cut(s) 1310, 3958
BstUI CGCG 1 cut(s) 88
BstV2I GAAGAC 3 cut(s) 890, 990, 2217
Bsu36I CCTNAGG 1 cut(s) 1473
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 7 cut(s) 22, 331, 600, 2719, 2744, 2769, 3547
BtsCI GGATG 4 cut(s) 55, 768, 2730, 2937
BtsI GCAGTG 2 cut(s) 882, 2321
BtsIMutI CAGTG 9 cut(s) 402, 882, 2312, 2321, 2371, 2698, 3256, 3270, 3634
BveI ACCTGC 2 cut(s) 2320, 3281
CaiI CAGNNNCTG 2 cut(s) 41, 2644
CciI TCATGA 4 cut(s) 738, 1776, 2122, 3385
CfoI GCGC 3 cut(s) 35, 1917, 2965
Cfr13I GGNCC 5 cut(s) 330, 2332, 2742, 3058, 3122
CsiI ACCWGGT 2 cut(s) 1957, 3301
Csp6I GTAC 6 cut(s) 524, 712, 1353, 1789, 1813, 3960
CviQI GTAC 6 cut(s) 524, 712, 1353, 1789, 1813, 3960
DriI GACNNNNNGTC 2 cut(s) 3354, 3517
EaeI YGGCCR 1 cut(s) 598
Eam1104I CTCTTC 2 cut(s) 119, 937
Eam1105I GACNNNNNGTC 2 cut(s) 3354, 3517
EarI CTCTTC 2 cut(s) 119, 937
Ecl136II GAGCTC 1 cut(s) 3053
Eco130I CCWWGG 4 cut(s) 1348, 1413, 3548, 3922
Eco147I AGGCCT 2 cut(s) 22, 3547
Eco24I GRGCYC 4 cut(s) 510, 1678, 2345, 3055
Eco31I GGTCTC 1 cut(s) 260
Eco32I GATATC 2 cut(s) 3090, 3625
Eco47I GGWCC 3 cut(s) 2332, 3058, 3122
Eco53kI GAGCTC 1 cut(s) 3053
Eco57I CTGAAG 8 cut(s) 884, 932, 1910, 2429, 2825, 3684, 3726, 3988
Eco81I CCTNAGG 1 cut(s) 1473
EcoICRI GAGCTC 1 cut(s) 3053
EcoNI CCTNNNNNAGG 3 cut(s) 27, 339, 2976
EcoO109I RGGNCCY 3 cut(s) 330, 2332, 2742
EcoRI GAATTC 2 cut(s) 577, 694
EcoRV GATATC 2 cut(s) 3090, 3625
EcoT14I CCWWGG 4 cut(s) 1348, 1413, 3548, 3922
EcoT22I ATGCAT 1 cut(s) 1293
EcoT38I GRGCYC 4 cut(s) 510, 1678, 2345, 3055
ErhI CCWWGG 4 cut(s) 1348, 1413, 3548, 3922
Esp3I CGTCTC 4 cut(s) 891, 1679, 1704, 3537
FalI AAGNNNNNCTT 4 cut(s) 930, 962, 1964, 1996
FauNDI CATATG 2 cut(s) 789, 2073
FblI GTMKAC 1 cut(s) 3361
FokI GGATG 4 cut(s) 62, 755, 2737, 2924
FriOI GRGCYC 4 cut(s) 510, 1678, 2345, 3055
FspBI CTAG 8 cut(s) 894, 1703, 1947, 2922, 3315, 3369, 3429, 3549
GlaI GCGC 3 cut(s) 34, 1916, 2964
GsaI CCCAGC 1 cut(s) 3018
GsuI CTGGAG 2 cut(s) 1931, 3536
HaeII RGCGCY 1 cut(s) 1918
HaeIII GGCC 7 cut(s) 22, 331, 600, 2719, 2744, 2769, 3547
HapII CCGG 4 cut(s) 144, 2720, 2801, 3830
HhaI GCGC 3 cut(s) 35, 1917, 2965
Hin1I GRCGYC 1 cut(s) 1688
Hin6I GCGC 3 cut(s) 33, 1915, 2963
HinP1I GCGC 3 cut(s) 33, 1915, 2963
HincII GTYRAC 3 cut(s) 2887, 3492, 3522
HindII GTYRAC 3 cut(s) 2887, 3492, 3522
HpaII CCGG 4 cut(s) 144, 2720, 2801, 3830
HphI GGTGA 9 cut(s) 502, 822, 2675, 2919, 2961, 3216, 3280, 3341, 3683
Hpy166II GTNNAC 5 cut(s) 2887, 3362, 3492, 3522, 3956
Hpy8I GTNNAC 5 cut(s) 2887, 3362, 3492, 3522, 3956
HpyCH4IV ACGT 5 cut(s) 1688, 1697, 3238, 3530, 3958
HpySE526I ACGT 5 cut(s) 1688, 1697, 3238, 3530, 3958
Hsp92I GRCGYC 1 cut(s) 1688
HspAI GCGC 3 cut(s) 33, 1915, 2963
LmnI GCTCC 5 cut(s) 66, 1912, 2064, 2841, 3050
MabI ACCWGGT 2 cut(s) 1957, 3301
MaeI CTAG 8 cut(s) 894, 1703, 1947, 2922, 3315, 3369, 3429, 3549
MaeII ACGT 5 cut(s) 1688, 1697, 3238, 3530, 3958
MfeI CAATTG 3 cut(s) 285, 2144, 2498
MhlI GDGCHC 7 cut(s) 510, 1310, 1678, 2345, 3055, 3695, 3958
MlyI GAGTC 5 cut(s) 511, 1444, 1841, 2350, 3527
MmeI TCCRAC 7 cut(s) 204, 517, 796, 2374, 3004, 3028, 3809
Mph1103I ATGCAT 1 cut(s) 1293
MroXI GAANNNNTTC 1 cut(s) 3904
MslI CAYNNNNRTG 2 cut(s) 1731, 3141
MspA1I CMGCKG 1 cut(s) 2644
MspI CCGG 4 cut(s) 144, 2720, 2801, 3830
MunI CAATTG 3 cut(s) 285, 2144, 2498
Mva1269I GAATGC 3 cut(s) 955, 2395, 2869
MvnI CGCG 1 cut(s) 88
NciI CCSGG 2 cut(s) 2721, 2802
NdeI CATATG 2 cut(s) 789, 2073
NheI GCTAGC 1 cut(s) 2921
NmuCI GTSAC 5 cut(s) 381, 1479, 2091, 2862, 3251
NsiI ATGCAT 1 cut(s) 1293
NspI RCATGY 5 cut(s) 1156, 1378, 1405, 2422, 3980
PaeI GCATGC 2 cut(s) 1378, 3980
PagI TCATGA 4 cut(s) 738, 1776, 2122, 3385
PasI CCCWGGG 1 cut(s) 3083
PceI AGGCCT 2 cut(s) 22, 3547
PcsI WCGNNNNNNNCGW 1 cut(s) 1694
PctI GAATGC 3 cut(s) 955, 2395, 2869
PdmI GAANNNNTTC 1 cut(s) 3904
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 2 cut(s) 1261, 3308
PfoI TCCNGGA 2 cut(s) 340, 2296
PleI GAGTC 5 cut(s) 511, 1443, 1841, 2350, 3526
PpsI GAGTC 5 cut(s) 511, 1443, 1841, 2350, 3526
Ppu21I YACGTR 2 cut(s) 3239, 3959
PpuMI RGGWCCY 1 cut(s) 2332
PshAI GACNNNNGTC 2 cut(s) 1462, 2360
PshBI ATTAAT 3 cut(s) 1259, 2010, 2436
Psp124BI GAGCTC 1 cut(s) 3055
Psp5II RGGWCCY 1 cut(s) 2332
PspFI CCCAGC 1 cut(s) 3014
PspPI GGNCC 5 cut(s) 330, 2332, 2742, 3058, 3122
PspPPI RGGWCCY 1 cut(s) 2332
PstI CTGCAG 5 cut(s) 877, 1581, 2328, 2427, 3325
PstNI CAGNNNCTG 2 cut(s) 41, 2644
PsyI GACNNNGTC 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 2644
RsaI GTAC 6 cut(s) 525, 713, 1354, 1790, 1814, 3961
RsaNI GTAC 6 cut(s) 524, 712, 1353, 1789, 1813, 3960
RseI CAYNNNNRTG 2 cut(s) 1731, 3141
SacI GAGCTC 1 cut(s) 3055
Sau96I GGNCC 5 cut(s) 330, 2332, 2742, 3058, 3122
SchI GAGTC 5 cut(s) 511, 1444, 1841, 2350, 3527
SduI GDGCHC 7 cut(s) 510, 1310, 1678, 2345, 3055, 3695, 3958
SexAI ACCWGGT 2 cut(s) 1957, 3301
SfcI CTRYAG 7 cut(s) 873, 1577, 2324, 2423, 2763, 3321, 3362
SinI GGWCC 3 cut(s) 2332, 3058, 3122
SmiMI CAYNNNNRTG 2 cut(s) 1731, 3141
SmlI CTYRAG 6 cut(s) 27, 167, 1369, 1880, 2109, 2516
SmoI CTYRAG 6 cut(s) 27, 167, 1369, 1880, 2109, 2516
SpeI ACTAGT 1 cut(s) 3428
SphI GCATGC 2 cut(s) 1378, 3980
SseBI AGGCCT 2 cut(s) 22, 3547
SspMI CTAG 8 cut(s) 894, 1703, 1947, 2922, 3315, 3369, 3429, 3549
SstI GAGCTC 1 cut(s) 3055
StuI AGGCCT 2 cut(s) 22, 3547
StyI CCWWGG 4 cut(s) 1348, 1413, 3548, 3922
TaiI ACGT 5 cut(s) 1691, 1700, 3241, 3533, 3961
TaqII GACCGA 1 cut(s) 3110
TatI WGTACW 1 cut(s) 1788
TauI GCSGC 7 cut(s) 91, 976, 1114, 2050, 3604, 3656, 4046
TscAI CASTG 9 cut(s) 402, 882, 2319, 2328, 2371, 2698, 3256, 3270, 3634
TseFI GTSAC 5 cut(s) 381, 1479, 2091, 2862, 3251
Tsp45I GTSAC 5 cut(s) 381, 1479, 2091, 2862, 3251
TspGWI ACGGA 2 cut(s) 250, 2259
TspRI CASTG 9 cut(s) 402, 882, 2319, 2328, 2371, 2698, 3256, 3270, 3634
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 2 cut(s) 1261, 3308
VneI GTGCAC 1 cut(s) 3954
VpaK11BI GGWCC 3 cut(s) 2332, 3058, 3122
VspI ATTAAT 3 cut(s) 1259, 2010, 2436
XagI CCTNNNNNAGG 3 cut(s) 27, 339, 2976
XbaI TCTAGA 2 cut(s) 1702, 3314
XceI RCATGY 5 cut(s) 1156, 1378, 1405, 2422, 3980
XmaJI CCTAGG 1 cut(s) 3548
XmiI GTMKAC 1 cut(s) 3361
XmnI GAANNNNTTC 1 cut(s) 3904
XspI CTAG 8 cut(s) 894, 1703, 1947, 2922, 3315, 3369, 3429, 3549
ZraI GACGTC 1 cut(s) 1689
Zsp2I ATGCAT 1 cut(s) 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.