Rh5AG500000

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006832
Physical Location & Seq
Forward (+)
84725374 .. 84733244
7871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG500000.1

Sequence Viewer

Length: 4059 bp
ATGGACACGAATAATTGGAGGCCTCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGAGCCAATTACAGCAGGATTCGCGGCAGAGGATCGTCAATAAGATAATGGATACATTGAAGAGGCATCTTCCCTTCTCCGGTCAAGAGGGATTGCATGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATACTGCTGCTACAAGCCAGTCGGATTATCTACGGAAAATTTCTCTGAAAATGCTCACTATGGAGACCAAGTCTCAGAACACAATTGGCAATTCTTTACCAAGCAACTCTGCTGGTAATAGTAACAGGCCCCCTGATCCAGGATCTTTCATGCAATCCCAAGTTCCCAATCAAGGACAGTCACTCCCTATGCCAGTGCCAGCCAATCAATCTCAACCACGCCAACAACTCTTATCACAGAACATTCAGAATAACATTCCGCCCAATTCTGCTGGCTTATCATCTGCATTATCTCCTGCATCTGGGCTCACCCAGACTCCTGTACCGAGTATTGTTGGAAATGCAAACTTGCAAAGTATGTCGGGGACATCACAGAATTCATTGGGGAACTCCATCGGCCAGGGGGTAACTTCAAATATGTTTGCTAATTCCCAAAGGCAAATGCCAGGACGACAACAGGTAGTTCCGCAGCAGCAACAGCAGCAAACCCAGAATTCACAGCAGTATATGTACCAGCAACAGTTACAACAACAGCTCATGAAGCCAAAATTTCAGCAGCAGGGAAACATCCAACTTGTGCAAAATCATATGCAACAGCAACAACAGCAGCAGCAGAACTTATTGCAATCACCTCAGTTGCAATCTTCTCAGCAATCTGTTATGCAACCTCCTGCAGTGCAGTCGTCTCTCTCTAGTCTTCAGCAGAATCAACAATCTGCTATGCAACACTCTTCACAAGCCATGCTTCAGCAGCATCCTCAATCTGTTCTGCGGCAGCAACAGCAGCCTCAACAGTCTTCTGTTGTTCATCAGCAGCAAACACCAATCCCACAGCAGCCAATACTGCCCCCACAGCAGCAGCAGCAGCAGCAACAGCCACAGCTTATGGTGCAACAGCCAAATTCAACAAACTTGCCGCAGAATCAGCTTATTGGACAACAAAACAATGTAGGGGACATGCAGCAACAGCAACAGCAACAGCAACAGCAAAGGTTACTAAGCCAGCAGAATAATATGTTAAACTTGCAACAGCAGCAGCAGCAACAACAACACCAATTAATGGCTCAGCAAAACAACCTCTCAAATATGCATCAGCAACAGTTGGGCACTCAGAGTAATGTTACTGGATTACAGCAACAGCAGCACCTTGGTACTCAAACTGGTAACTCAAGCATGCAAACTAATCAGCACTCTGCACACATGCTGCAATCCAAGGTTCAGCAACAACATCAGAGTCCAGCATTACCGATTCAAGGACATCAGTCCCAACCTCAGGTGTCACAGCAACAATTATTATCACAGATTCAATCTCAGCCTGCACAGATACAACAACAGATGGGTATGCAACAGCAATCAAACCAGCTACAACGAGATATGCAGCAAAGGATCCAAGCATCAGGTCAGGTATCAGGATCCATGCTTCAACCACCAAATGTAATGGATCCGCAAAAGCAGTTATATCAAGCTCAAAGAGCCCTTCCTGAGACGTCATCAACGTCTCTAGATTCCACAGCCCAGACTGGACATACAAATGGTGCTGATTGGCAAGAGGAGGTCTATCAAAAGATCAAAGTCATGAAGGATATGTACTACCCCGAACTAAGTGAAATGTACCAAAAAATTGCTACTAAACTTCATCAGCACGACTCCCTTCCACAACAGCCAAAGTCAGATCAGCTTGAGAAGCTGAAGATGTTTAAGACCATGCTGGAGCGCCTGATAACAGTCTTACAAATTTCCAAAACTAGCATAACACCTGGTTTGAAGGAGAAGTTGGGTTTATACGAGAAGCAGATAATAAATTTTATTAATACAAATAGACCCAGGAAGCCTGTTTCTTCTCTGCCGCCAGGGCAGCTCCCCCCACCGCATATGCAGCCCATGCAGCAGTCACAATCCCAGATTACTCAAGTGCAATCTCATGAAAATCAGATGAACCCACAATTGCAATCCCTGAACTTGCAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACGGGCTTGCAGCAAAATTCTATGTCTTCTTTATCTGGGGTTTCAACAGCACAGCAAAACATGATGAATCCGTTGCAGCCAAGTTCCAATATGGATCCAGGACAAGGAACCGCACTGAACTCACTGCAGCAGGTCCCTGTGGGCTCTATCCAACAGACTCCTGTCAGTGCTCCCCAACAAGCTAACATGAATGCCTTATCATCACAGAATGGGATTAACATGCTGCAGAGCAACATTAATCCCCTTCAGTCAAATTCTGGTATGCTTCATAGCCAACATCTGAAACAGCAGGAACAGCAATTGTATCAAAATCAACTCAAGCAACAACAATTTCAACAACGACAGATGCAGCAGCAGCAGCAGCAGCAGTTGATGCAGAAGCAGATAATTCAACAACAGCAGCAGCAGCAACAGCAACAGCAACAACAGCTACACCAGCAGGCAAAGCAGCAGCTGCCTGGACAGTTGCAGGCACACCAAATGCCACAGCTTCACCAAATCAGTGATGTTAATGATGTGAAAATAAGGCCGGGGATGGGTGTTAAGTCAGGGGCCTTTCAGCAACAAATGTCTGTAGGCCAGCGTGCTTATCCTCATTCGCAATTAAAACCGGGATCTCCATATCCTATCACATCAAGCAACCAACTCCTTCAGGCTCAATCTCCTCAACTGTCACAGCATTCTTCTCCACAAGTTGACCAGCCGATGTTAGTCCCAAAAGCTCAAAATGCTAGTTCACCATTTGTCATCCCATCTCCTTCAACTCCCATAGCTCCATCACCTATGCCAGGAGATTCAGAGAAACCTTCCTCACTCGCAAATGCTGGGAATGTTGGACATCAACAAACAAATGTTGTCGGAGCTCAGGTCCAGTCCCTTGCCATCGGCACCCCTGGGATATCAGCCTCTCCTTTACTTGCTGAATTTATCGGTCCAGATGGCACTCATGCTAATGCTTTGTCAGCTATATCTGGCAAGTCAAGTGTTACAGAGCAACCTCTTGAACGCTTGATTAAAGCGGTCAAGTCAATATCACCAAAGGCATTAAGTGCATCTGTCAGTGACATAGGCTCAGTAGTGAGTATGATTGATAGGATAGCAGGTTCAGCACCAGGTAATGGATCTAGAGCTGCAGTTGGTGAGGATCTGGTTGCTATGACAAAATGTCGTCTACAGGCGAGAAACTTCATCACTCATGATGGAACAACAAATGGGACAAGGAAAATGAGGCGATACACTAGTGCCATGCCCTTAAATGTTGTGTCATCAGGTGGCAGCATGAATGATAGTTTCAAGCAGTTGACCTGTTCAGAGACATCTGACCTGGAGTCAACTGCAACGTCTCGTATCAAAAGGCCTAGGATTGAGGCTAATCATGCTCTTATGGAAGAAATAAGAGAAATAAATCGGCGGCTTATAGATACAGTTGTTGATATAAGTGATGAAGATGTTGATCCAAGTGCGGCTGCTGCTGAAGGGGGTGAAGGAACCATTGTCAAGTGCTCTTTTGATGCTGTGGCTCTCAGTCCCAACTTGAAATCGCAGTATGCTTCAGCACAAATGTCTCCGATTCAGCCATTAAGACTGCTTGTTCCGACAAATTATCCCAACTGCTCTCCAATACTGTTAGACAAGTTTCCGGTTGAAGTCAGTAAGGAGTATGAAGATCTTTCTGTGAAGGCAAAATCAAGGTTTAGCATCTCTTTACGAAGCATTTCGCAGCCCATGTCCCTTGGGGAGATAGCAAGGGCTTGGGATAATTGTGCACGTACAGTTATTTCTGAGCATGCACAGCGAAGTGGTGGAGGCAGCTTCAGCTCAAAGTATGGGACATGGGAGAACTGCCTGAGTGCCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1352

Amino Acids

149.4

Weight (kDa)

9.4

Isoelectric Point (pI)

71.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 98 1.4e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1691
Acc36I ACCTGC 2 cut(s) 2320, 3290
AccB1I GGYRCC 1 cut(s) 3086
AccB7I CCANNNNNTGG 2 cut(s) 1261, 3317
AccI GTMKAC 1 cut(s) 3370
AccII CGCG 1 cut(s) 88
AcoI YGGCCR 1 cut(s) 598
AcuI CTGAAG 8 cut(s) 884, 932, 1910, 2429, 2834, 3693, 3735, 3997
AcyI GRCGYC 1 cut(s) 1688
AfaI GTAC 6 cut(s) 525, 713, 1354, 1790, 1814, 3970
AhdI GACNNNNNGTC 2 cut(s) 3363, 3526
AhlI ACTAGT 1 cut(s) 3437
AjuI GAANNNNNNNTTGG 2 cut(s) 1958, 1990
AloI GAACNNNNNNTCC 2 cut(s) 3286, 3318
Alw21I GWGCWC 4 cut(s) 2371, 3064, 3704, 3967
Alw26I GTCTC 8 cut(s) 260, 279, 891, 1679, 1704, 3506, 3546, 3768
Alw44I GTGCAC 1 cut(s) 3963
AlwNI CAGNNNCTG 1 cut(s) 2653
AoxI GGCC 7 cut(s) 20, 329, 598, 2726, 2751, 2776, 3554
ApaLI GTGCAC 1 cut(s) 3963
AseI ATTAAT 3 cut(s) 1259, 2010, 2436
Asp700I GAANNNNTTC 1 cut(s) 3913
AspA2I CCTAGG 1 cut(s) 3557
AspLEI GCGC 1 cut(s) 1917
AspS9I GGNCC 5 cut(s) 330, 2332, 2751, 3067, 3131
AsuC2I CCSGG 2 cut(s) 2730, 2811
AsuHPI GGTGA 8 cut(s) 502, 822, 2684, 2928, 2970, 3225, 3350, 3692
AvaII GGWCC 3 cut(s) 2332, 3067, 3131
AvrII CCTAGG 1 cut(s) 3557
AxyI CCTNAGG 1 cut(s) 1473
BaeGI GKGCMC 2 cut(s) 1310, 3967
BaeI ACNNNNGTAYC 6 cut(s) 507, 540, 3424, 3457, 3612, 3645
BamHI GGATCC 4 cut(s) 1587, 1613, 1642, 2293
BanI GGYRCC 1 cut(s) 3086
BanII GRGCYC 4 cut(s) 510, 1678, 2345, 3064
BarI GAAGNNNNNNTAC 2 cut(s) 1772, 1804
BbsI GAAGAC 3 cut(s) 890, 990, 2217
Bbv12I GWGCWC 4 cut(s) 2371, 3064, 3704, 3967
BccI CCATC 8 cut(s) 602, 1531, 2728, 2959, 2983, 3089, 3131, 3392
BcgI CGANNNNNNTGC 2 cut(s) 864, 898
BciVI GTATCC 1 cut(s) 109
BcnI CCSGG 2 cut(s) 2730, 2811
BcoDI GTCTC 8 cut(s) 260, 279, 891, 1679, 1704, 3506, 3546, 3768
BcuI ACTAGT 1 cut(s) 3437
BfaI CTAG 7 cut(s) 894, 1703, 1947, 2931, 3324, 3438, 3558
BfmI CTRYAG 6 cut(s) 873, 2324, 2423, 2772, 3330, 3371
BfoI RGCGCY 1 cut(s) 1918
BfuAI ACCTGC 2 cut(s) 2320, 3290
BfuI GTATCC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 2053
BglII AGATCT 1 cut(s) 3865
BlnI CCTAGG 1 cut(s) 3557
BlpI GCTNAGC 1 cut(s) 1266
Bme18I GGWCC 3 cut(s) 2332, 3067, 3131
BmeRI GACNNNNNGTC 2 cut(s) 3363, 3526
BmgT120I GGNCC 5 cut(s) 330, 2332, 2751, 3067, 3131
BoxI GACNNNNGTC 2 cut(s) 1462, 2360
BpiI GAAGAC 3 cut(s) 890, 990, 2217
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 2 cut(s) 1931, 3545
Bpu10I CCTNAGC 1 cut(s) 3063
Bpu1102I GCTNAGC 1 cut(s) 1266
BpuEI CTTGAG 6 cut(s) 12, 152, 1354, 1901, 2094, 2501
BpuMI CCSGG 2 cut(s) 2730, 2811
BsaAI YACGTR 1 cut(s) 3968
BsaBI GATNNNNATC 1 cut(s) 3651
BsaHI GRCGYC 1 cut(s) 1688
BsaI GGTCTC 1 cut(s) 260
BsaWI WCCGGW 2 cut(s) 143, 3838
BsaXI ACNNNNNCTCC 8 cut(s) 257, 287, 369, 399, 502, 532, 3051, 3081
Bse1I ACTGG 6 cut(s) 220, 395, 1330, 1366, 1726, 3070
Bse21I CCTNAGG 1 cut(s) 1473
Bse3DI GCAATG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 3651
BseGI GGATG 5 cut(s) 55, 768, 955, 2739, 2946
BseJI GATNNNNATC 1 cut(s) 3651
BseMI GCAATG 1 cut(s) 51
BseNI ACTGG 6 cut(s) 220, 395, 1330, 1366, 1726, 3070
BseRI GAGGAG 4 cut(s) 15, 79, 1766, 2853
BseSI GKGCMC 2 cut(s) 1310, 3967
BseYI CCCAGC 1 cut(s) 3023
BsgI GTGCAG 3 cut(s) 899, 1380, 1503
Bsh1236I CGCG 1 cut(s) 88
BshFI GGCC 7 cut(s) 22, 331, 600, 2728, 2753, 2778, 3556
BshNI GGYRCC 1 cut(s) 3086
BsiHKAI GWGCWC 4 cut(s) 2371, 3064, 3704, 3967
BsiSI CCGG 4 cut(s) 144, 2729, 2810, 3839
BsmAI GTCTC 8 cut(s) 260, 279, 891, 1679, 1704, 3506, 3546, 3768
BsmBI CGTCTC 4 cut(s) 891, 1679, 1704, 3546
BsmI GAATGC 2 cut(s) 2395, 2878
BsnI GGCC 7 cut(s) 22, 331, 600, 2728, 2753, 2778, 3556
Bso31I GGTCTC 1 cut(s) 260
Bsp1286I GDGCHC 8 cut(s) 510, 1310, 1678, 2345, 2371, 3064, 3704, 3967
Bsp1720I GCTNAGC 1 cut(s) 1266
BspANI GGCC 7 cut(s) 22, 331, 600, 2728, 2753, 2778, 3556
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 4 cut(s) 738, 1776, 2122, 3394
BspMAI CTGCAG 4 cut(s) 877, 2328, 2427, 3334
BspMI ACCTGC 2 cut(s) 2320, 3290
BspT107I GGYRCC 1 cut(s) 3086
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 6 cut(s) 220, 395, 1330, 1366, 1726, 3070
BssNI GRCGYC 1 cut(s) 1688
BssT1I CCWWGG 4 cut(s) 1348, 1413, 3557, 3931
Bst6I CTCTTC 2 cut(s) 119, 937
BstACI GRCGYC 1 cut(s) 1688
BstAPI GCANNNNNTGC 5 cut(s) 50, 2083, 2572, 2653, 3248
BstBAI YACGTR 1 cut(s) 3968
BstENI CCTNNNNNAGG 3 cut(s) 27, 339, 2985
BstF5I GGATG 5 cut(s) 55, 768, 955, 2739, 2946
BstFNI CGCG 1 cut(s) 88
BstH2I RGCGCY 1 cut(s) 1918
BstHHI GCGC 1 cut(s) 1917
BstMAI GTCTC 8 cut(s) 260, 279, 891, 1679, 1704, 3506, 3546, 3768
BstNSI RCATGY 5 cut(s) 1162, 1378, 1405, 2422, 3989
BstPAI GACNNNNGTC 2 cut(s) 1462, 2360
BstSFI CTRYAG 6 cut(s) 873, 2324, 2423, 2772, 3330, 3371
BstSLI GKGCMC 2 cut(s) 1310, 3967
BstUI CGCG 1 cut(s) 88
BstV2I GAAGAC 3 cut(s) 890, 990, 2217
BstX2I RGATCY 9 cut(s) 344, 1587, 1613, 1642, 2293, 2813, 3320, 3343, 3865
BstYI RGATCY 9 cut(s) 344, 1587, 1613, 1642, 2293, 2813, 3320, 3343, 3865
Bsu36I CCTNAGG 1 cut(s) 1473
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 7 cut(s) 22, 331, 600, 2728, 2753, 2778, 3556
BtsCI GGATG 5 cut(s) 55, 768, 955, 2739, 2946
BtsI GCAGTG 2 cut(s) 882, 2321
BtsIMutI CAGTG 7 cut(s) 402, 882, 2312, 2321, 2371, 2707, 3265
BveI ACCTGC 2 cut(s) 2320, 3290
CaiI CAGNNNCTG 1 cut(s) 2653
CciI TCATGA 4 cut(s) 738, 1776, 2122, 3394
CfoI GCGC 1 cut(s) 1917
Cfr13I GGNCC 5 cut(s) 330, 2332, 2751, 3067, 3131
CsiI ACCWGGT 2 cut(s) 1957, 3310
Csp6I GTAC 6 cut(s) 524, 712, 1353, 1789, 1813, 3969
CviQI GTAC 6 cut(s) 524, 712, 1353, 1789, 1813, 3969
DriI GACNNNNNGTC 2 cut(s) 3363, 3526
EaeI YGGCCR 1 cut(s) 598
Eam1104I CTCTTC 2 cut(s) 119, 937
Eam1105I GACNNNNNGTC 2 cut(s) 3363, 3526
EarI CTCTTC 2 cut(s) 119, 937
EciI GGCGGA 1 cut(s) 450
Ecl136II GAGCTC 1 cut(s) 3062
Eco130I CCWWGG 4 cut(s) 1348, 1413, 3557, 3931
Eco147I AGGCCT 2 cut(s) 22, 3556
Eco24I GRGCYC 4 cut(s) 510, 1678, 2345, 3064
Eco31I GGTCTC 1 cut(s) 260
Eco32I GATATC 1 cut(s) 3099
Eco47I GGWCC 3 cut(s) 2332, 3067, 3131
Eco53kI GAGCTC 1 cut(s) 3062
Eco57I CTGAAG 8 cut(s) 884, 932, 1910, 2429, 2834, 3693, 3735, 3997
Eco81I CCTNAGG 1 cut(s) 1473
EcoICRI GAGCTC 1 cut(s) 3062
EcoNI CCTNNNNNAGG 3 cut(s) 27, 339, 2985
EcoO109I RGGNCCY 3 cut(s) 330, 2332, 2751
EcoRI GAATTC 2 cut(s) 577, 694
EcoRV GATATC 1 cut(s) 3099
EcoT14I CCWWGG 4 cut(s) 1348, 1413, 3557, 3931
EcoT22I ATGCAT 1 cut(s) 1293
EcoT38I GRGCYC 4 cut(s) 510, 1678, 2345, 3064
ErhI CCWWGG 4 cut(s) 1348, 1413, 3557, 3931
Esp3I CGTCTC 4 cut(s) 891, 1679, 1704, 3546
FalI AAGNNNNNCTT 2 cut(s) 930, 962
FauNDI CATATG 2 cut(s) 789, 2073
FblI GTMKAC 1 cut(s) 3370
FokI GGATG 5 cut(s) 62, 755, 942, 2746, 2933
FriOI GRGCYC 4 cut(s) 510, 1678, 2345, 3064
FspBI CTAG 7 cut(s) 894, 1703, 1947, 2931, 3324, 3438, 3558
GlaI GCGC 1 cut(s) 1916
GsaI CCCAGC 1 cut(s) 3027
GsuI CTGGAG 2 cut(s) 1931, 3545
HaeII RGCGCY 1 cut(s) 1918
HaeIII GGCC 7 cut(s) 22, 331, 600, 2728, 2753, 2778, 3556
HapII CCGG 4 cut(s) 144, 2729, 2810, 3839
HhaI GCGC 1 cut(s) 1917
Hin1I GRCGYC 1 cut(s) 1688
Hin6I GCGC 1 cut(s) 1915
HinP1I GCGC 1 cut(s) 1915
HincII GTYRAC 3 cut(s) 2896, 3501, 3531
HindII GTYRAC 3 cut(s) 2896, 3501, 3531
HpaII CCGG 4 cut(s) 144, 2729, 2810, 3839
HphI GGTGA 8 cut(s) 502, 822, 2684, 2928, 2970, 3225, 3350, 3692
Hpy166II GTNNAC 6 cut(s) 2896, 2936, 3371, 3501, 3531, 3965
Hpy8I GTNNAC 6 cut(s) 2896, 2936, 3371, 3501, 3531, 3965
HpyCH4IV ACGT 4 cut(s) 1688, 1697, 3539, 3967
HpySE526I ACGT 4 cut(s) 1688, 1697, 3539, 3967
Hsp92I GRCGYC 1 cut(s) 1688
HspAI GCGC 1 cut(s) 1915
LmnI GCTCC 6 cut(s) 66, 1912, 2064, 2374, 2977, 3059
MabI ACCWGGT 2 cut(s) 1957, 3310
MaeI CTAG 7 cut(s) 894, 1703, 1947, 2931, 3324, 3438, 3558
MaeII ACGT 4 cut(s) 1688, 1697, 3539, 3967
MfeI CAATTG 3 cut(s) 285, 2144, 2498
MflI RGATCY 9 cut(s) 344, 1587, 1613, 1642, 2293, 2813, 3320, 3343, 3865
MhlI GDGCHC 8 cut(s) 510, 1310, 1678, 2345, 2371, 3064, 3704, 3967
MlyI GAGTC 5 cut(s) 511, 1444, 1841, 2350, 3536
MmeI TCCRAC 7 cut(s) 204, 517, 796, 2374, 3013, 3037, 3818
Mph1103I ATGCAT 1 cut(s) 1293
MroXI GAANNNNTTC 1 cut(s) 3913
MslI CAYNNNNRTG 2 cut(s) 1731, 3150
MspA1I CMGCKG 1 cut(s) 2653
MspI CCGG 4 cut(s) 144, 2729, 2810, 3839
MunI CAATTG 3 cut(s) 285, 2144, 2498
Mva1269I GAATGC 2 cut(s) 2395, 2878
MvnI CGCG 1 cut(s) 88
NciI CCSGG 2 cut(s) 2730, 2811
NdeI CATATG 2 cut(s) 789, 2073
NmuCI GTSAC 5 cut(s) 381, 1479, 2091, 2871, 3260
NsiI ATGCAT 1 cut(s) 1293
NspI RCATGY 5 cut(s) 1162, 1378, 1405, 2422, 3989
PaeI GCATGC 2 cut(s) 1378, 3989
PagI TCATGA 4 cut(s) 738, 1776, 2122, 3394
PasI CCCWGGG 1 cut(s) 3092
PceI AGGCCT 2 cut(s) 22, 3556
PcsI WCGNNNNNNNCGW 1 cut(s) 1694
PctI GAATGC 2 cut(s) 2395, 2878
PdmI GAANNNNTTC 1 cut(s) 3913
PfeI GAWTC 9 cut(s) 83, 907, 1123, 1450, 1504, 1706, 2266, 2993, 3769
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 2 cut(s) 1261, 3317
PfoI TCCNGGA 2 cut(s) 340, 2296
PleI GAGTC 5 cut(s) 511, 1443, 1841, 2350, 3535
PpsI GAGTC 5 cut(s) 511, 1443, 1841, 2350, 3535
Ppu21I YACGTR 1 cut(s) 3968
PpuMI RGGWCCY 1 cut(s) 2332
PshAI GACNNNNGTC 2 cut(s) 1462, 2360
PshBI ATTAAT 3 cut(s) 1259, 2010, 2436
Psp124BI GAGCTC 1 cut(s) 3064
Psp5II RGGWCCY 1 cut(s) 2332
PspFI CCCAGC 1 cut(s) 3023
PspPI GGNCC 5 cut(s) 330, 2332, 2751, 3067, 3131
PspPPI RGGWCCY 1 cut(s) 2332
PstI CTGCAG 4 cut(s) 877, 2328, 2427, 3334
PstNI CAGNNNCTG 1 cut(s) 2653
PsuI RGATCY 9 cut(s) 344, 1587, 1613, 1642, 2293, 2813, 3320, 3343, 3865
PsyI GACNNNGTC 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 2653
RsaI GTAC 6 cut(s) 525, 713, 1354, 1790, 1814, 3970
RsaNI GTAC 6 cut(s) 524, 712, 1353, 1789, 1813, 3969
RseI CAYNNNNRTG 2 cut(s) 1731, 3150
SacI GAGCTC 1 cut(s) 3064
Sau96I GGNCC 5 cut(s) 330, 2332, 2751, 3067, 3131
SchI GAGTC 5 cut(s) 511, 1444, 1841, 2350, 3536
SduI GDGCHC 8 cut(s) 510, 1310, 1678, 2345, 2371, 3064, 3704, 3967
SexAI ACCWGGT 2 cut(s) 1957, 3310
SfcI CTRYAG 6 cut(s) 873, 2324, 2423, 2772, 3330, 3371
SinI GGWCC 3 cut(s) 2332, 3067, 3131
SmiMI CAYNNNNRTG 2 cut(s) 1731, 3150
SmlI CTYRAG 6 cut(s) 27, 167, 1369, 1880, 2109, 2516
SmoI CTYRAG 6 cut(s) 27, 167, 1369, 1880, 2109, 2516
SpeI ACTAGT 1 cut(s) 3437
SphI GCATGC 2 cut(s) 1378, 3989
SseBI AGGCCT 2 cut(s) 22, 3556
SspMI CTAG 7 cut(s) 894, 1703, 1947, 2931, 3324, 3438, 3558
SstI GAGCTC 1 cut(s) 3064
StuI AGGCCT 2 cut(s) 22, 3556
StyI CCWWGG 4 cut(s) 1348, 1413, 3557, 3931
TaiI ACGT 4 cut(s) 1691, 1700, 3542, 3970
TaqII GACCGA 1 cut(s) 3119
TatI WGTACW 1 cut(s) 1788
TauI GCSGC 7 cut(s) 91, 976, 1120, 2050, 3613, 3665, 4055
TfiI GAWTC 9 cut(s) 83, 907, 1123, 1450, 1504, 1706, 2266, 2993, 3769
TscAI CASTG 7 cut(s) 402, 882, 2319, 2328, 2371, 2707, 3265
TseFI GTSAC 5 cut(s) 381, 1479, 2091, 2871, 3260
Tsp45I GTSAC 5 cut(s) 381, 1479, 2091, 2871, 3260
TspGWI ACGGA 2 cut(s) 250, 2259
TspRI CASTG 7 cut(s) 402, 882, 2319, 2328, 2371, 2707, 3265
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 2 cut(s) 1261, 3317
VneI GTGCAC 1 cut(s) 3963
VpaK11BI GGWCC 3 cut(s) 2332, 3067, 3131
VspI ATTAAT 3 cut(s) 1259, 2010, 2436
XagI CCTNNNNNAGG 3 cut(s) 27, 339, 2985
XbaI TCTAGA 2 cut(s) 1702, 3323
XceI RCATGY 5 cut(s) 1162, 1378, 1405, 2422, 3989
XmaJI CCTAGG 1 cut(s) 3557
XmiI GTMKAC 1 cut(s) 3370
XmnI GAANNNNTTC 1 cut(s) 3913
XspI CTAG 7 cut(s) 894, 1703, 1947, 2931, 3324, 3438, 3558
ZraI GACGTC 1 cut(s) 1689
Zsp2I ATGCAT 1 cut(s) 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.