Rh5CG545300

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0006832
Physical Location & Seq
Forward (+)
76081302 .. 76089145
7844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG545300.1

Sequence Viewer

Length: 3801 bp
ATGGACACGAATAATTGGAGGCCTCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGAGCCAATTACAGCAGGATTCGCGGCAGAGAATCGTCAATAAGATAATGGATACATTGAAGAGGCATCTTCCCTTCTCCGGTCAAGAGGGATTGCACGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATACTGCTGCTACAAGCCAGTCGGATTATCTACGGAAAATTTCTCTGAAAATGCTCACTATGGAGACCAAGTCTCAGAACACAATTGGCACTTCTTTACCAAGCAACTCTGCTGGTAATAGTAACAGGCCCCCTGATCCAGGATCTTTCATGCAACCCCAAGTTCCCAATCAAGGACAGTCACTCCCTATGCCAGTGCCAGCCAATCAATCTCAACCACGCCAACAACTCTTATCACAGAACATTCAGAATAACATTCCGCCCAATTCTGCTGGCTTATCATCTGCATTATCTCCTGCATCTGGGCTCACCCAGACTCCTGTACCGAGTATTGTTGGAAATGCAAACTTGCAAAGTATGTCGGGGACATCACAGAATTCATTGGGGAACTCCATCGGCCAGGGGGTAACTTCAAATATGTTTGCTAATTCCCAAAGGCAAATGCCAGGACGACAACAGGTAGTTCCGCAGCAGCAACAGCAGCAAACCCAGAATTCACAGCAGTATATGTACCAGCAACAGTTACAACAACAGCTCATGAAGCCAAAATTTCAGCAGCAGGGAAACATCCAACTTGTGCAAAATCATATGCAACAGCAACAACAGCAGCAGCAGAACTTATTGCAATCACCTCAGTTGCAATCTTCTCAGCAATCTGTTATGCAACCTCCTGCAGTGCAGTCGTCTCTCTCTAGTCTTCAGCAGAATCAACAATCTGCTATGCAACACTCTTCACAAGCCATGCTTCAGCAGCATTCTCAATCTGTTCTGCGGCAGCAACAGCAGCCTCAACAGTCTTCTGTTGTTCATCAGCAGCAAACACCAATCCCACAGCAGTCAATACTGCCCCCACAGCAGCAGCAGCAACAGCCACAGCTTATGGTGCAACAGCCAAATTCAACAAACTTGCCGCAGAATCAGCTTATTGGACAACAAAACAATGTAGGGGACATGCAGCAACAGCAACAGCAACAGCAACAGCAACAGCAACAGCAACAGCAACAGCAACAGCAACAGCAACAGCAAAGGTTACTAAGCCAGCAGAATAATATGTTAAACTTGCAACAGCAGCAGCAGCAACAACAACACCAATTAATGGCTCAGCAAAACAACCTCTCAAATATGCATCAGCAACAGTTGGGCACTCAGAGTAATGTTACTGGATTACAGCAACAGCAGCACCTTGGTACTCAAACTGGTAACTCAAGCATGCAAACTAATCAGCACTCTGCACACATGCTGCAATCCAAGGTTCAGCAACAACATCAGAGTCCAGCATTACCGATTCAAGGACATCAGTCCCAACCTCAGGTGTCACAGCAACAATTATTATCACAGATTCAATCTCAGCCTGCACAGATACAACAACAGATGGGTATGCAACAGCAATCAAACCAGCTACAACGAGATCTGCAGCAAAGGATCCAAGCATCAGGTCAGGTATCAGGATCCATGCTTCAACCACCAAATGTAATGGATCCGCAAAAGCAGTTATATCAAGCTCAAAGAGCCCTTCCTGAGACGTCATCAACGTCTCTAGATTCCACAGCCCAGACTGGACATACAAATGGTGCTGATTGGCAAGAGGAGGTCTATCAAAAGATCAAAGTCATGAAGGATATGTACTACCCCGAACTAAGTGAAATGTACCAAAAAATTGCTACTAAACTTCATCAGCACGACTCCCTTCCACAACAGCCAAAGTCAGATCAGCTTGAGAAGCTGAAGATGTTTAAGACCATGCTGGAGCGCCTGATAACAGTCTTACAAATTTCCAAAACTAGCATAACACCTGGTTTGAAGGAGAAGTTGGGCTTATACGAGAAGCAGATAATAAATTTTATTAATACAAATAGACCCAGGAAGCCTGTTTCTTCTCTGCCGCCAGGGCAGCTCCCCCCACCGCATATGCAGTCCATGCAGCAGTCACAATCCCAGATTACTCAAGTGCAATCTCATGAAAATCAGATGAACCCACAATTGCAATCCCTGAACTTGCAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACGGGCTTGCAGCAAAATTCTATGTCTTCTTTATCTGGGGTTTCAACAGCACAGCAAAACATGATGAATCCGTTGCAGCCAAGTTCCAATATGGATCCAGGACAAGGAACCGCACTGAACTCACTGCAGCAGGTCCCTGTGGGCTCTATCCAACAGACTCCTGTCAGTGCTCCCCAACAAGCTAACATGAATGCCTTATCATCACAGAATGGGATTAACATGCTGCAGAGCAACATTAATCCCCTTCAGTCAAATTCTGGTATGCTTCATAGCCAACATCTGAAACAGCAGGAACAGCAATTGTATCAGAATCAACTCAAGCAACAACAATTTCAACAACGACAGATGCAGCAGCAGCAGTTGATGCAGAAGCAGCTAATTCAACAACAGCAGCAGCAGCAACAGCAACAGCAACAACAGCTACAACAGCAAGCAAAGCAGCAGCTGCCTGGACAGTTGCAGGCACACCAAATGCCACAGCTTCACCAAATCAGTGATGTTAATGATGTGAAAGTAAGGCCGGGGATGGGTGTTAAGTCAGGGGCCTTTCAGCAACAAATGTCTGTAGGCCAGCGTGCTTATCCTCATTCGCAATTAAAACCGGGATCTCCATATCCTATCACATCAAGCAACCAACTCCTTCAGGCTCAATCTCCTCAACTGTCACAGCATTCTTCTCCACAAGTTGACCAGCCGATGTTAGTCCCAAAAGCTCAAAATGCTAGCTCACCATTTGTCATCCCATCTCCTTCAACTCCCATAGCGCCATCACCTATGCCAGGAGATTCAGAGAAACCTTCCTCACTCGCAAATGCTGGGAATGTTGGACATCAACAAGCAACTGTTGTCGGAGCTCAGGTCCAGTCCCTTGCCATCGGCACCCCTGGGATATCAGCCTCTCCTTTGCTTGCTGAATTTATCGGTCCAGATGGCACTCATGCTAATGCTTTGTCAGCAATATCTGGCAAGTCAAGTGTTACAGAGCAACCTCTTGAACGCTTGATTAAAGCGGTCAAGTCAATATCACCAAAGGCATTAAGTGCATCTGTCAGTGACATAGGCTCAGTAGTGAGTATGATTGATAGGATAGCAGGTTCAGCACCAGGTAATGGATCTAGAGCTGCAGTTGGTGAGGATCTGGTTGCTATGACAAAATGTCGTCTACAGGCGAGAAACTTCATCACTCATGATGGAACAACAAATGGGACAAGGAAAATGAGGCGATACACTAGTGCCATGCCCTTAAATGTTGTGTCATCAGGTGGCAGCATGAATGATAGTTTCAAGCAGTTGACCTGTTCAGAGACATCTGACCTGGAGTCAACTGCAACGTCTCGTATCAAAAGGCCTAGGATTGAGGCTAATCATGCTCTTATGGAAGAAATAAGAGAAATAAATCGGCGGCTTATAGATACAGTTGTTGATATCAGTGATGAAGATGTTGATCCAAGTGCGGCTGCTGCTGAAGGGGGTGAAGGAACCATCGTCAAGTGCTCTTTTGATGCTGTGGCTCTCAGTCCCAACTTGAAATCGCAGTATGCTTCAGCACAAATGGTTGGTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1266

Amino Acids

139.92

Weight (kDa)

9.47

Isoelectric Point (pI)

73.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 97 2e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1727
Acc36I ACCTGC 2 cut(s) 2356, 3317
AccB1I GGYRCC 1 cut(s) 3113
AccB7I CCANNNNNTGG 2 cut(s) 1297, 3344
AccI GTMKAC 1 cut(s) 3397
AccII CGCG 1 cut(s) 88
AcoI YGGCCR 1 cut(s) 598
AcuI CTGAAG 7 cut(s) 884, 932, 1946, 2465, 2861, 3720, 3762
AcyI GRCGYC 1 cut(s) 1724
AfaI GTAC 5 cut(s) 525, 713, 1390, 1826, 1850
AhdI GACNNNNNGTC 2 cut(s) 3390, 3553
AhlI ACTAGT 1 cut(s) 3464
AjuI GAANNNNNNNTTGG 2 cut(s) 1994, 2026
AloI GAACNNNNNNTCC 2 cut(s) 3313, 3345
Alw21I GWGCWC 3 cut(s) 2407, 3091, 3731
Alw26I GTCTC 7 cut(s) 260, 279, 891, 1715, 1740, 3533, 3573
AlwNI CAGNNNCTG 1 cut(s) 2680
AoxI GGCC 7 cut(s) 20, 329, 598, 2753, 2778, 2803, 3581
ArsI GACNNNNNNTTYG 1 cut(s) 3778
AseI ATTAAT 3 cut(s) 1295, 2046, 2472
AspA2I CCTAGG 1 cut(s) 3584
AspLEI GCGC 2 cut(s) 1953, 3001
AspS9I GGNCC 6 cut(s) 330, 2368, 2778, 3094, 3158, 3794
AsuC2I CCSGG 2 cut(s) 2757, 2838
AsuHPI GGTGA 8 cut(s) 502, 822, 2711, 2955, 2997, 3252, 3377, 3719
AsuNHI GCTAGC 1 cut(s) 2957
AvaII GGWCC 4 cut(s) 2368, 3094, 3158, 3794
AvrII CCTAGG 1 cut(s) 3584
AxyI CCTNAGG 1 cut(s) 1509
BaeGI GKGCMC 1 cut(s) 1346
BaeI ACNNNNGTAYC 6 cut(s) 507, 540, 3451, 3484, 3639, 3672
BamHI GGATCC 4 cut(s) 1623, 1649, 1678, 2329
BanI GGYRCC 1 cut(s) 3113
BanII GRGCYC 4 cut(s) 510, 1714, 2381, 3091
BarI GAAGNNNNNNTAC 2 cut(s) 1808, 1840
BbsI GAAGAC 3 cut(s) 890, 990, 2253
Bbv12I GWGCWC 3 cut(s) 2407, 3091, 3731
BccI CCATC 9 cut(s) 602, 1567, 2755, 2986, 3010, 3116, 3158, 3419, 3725
BcgI CGANNNNNNTGC 2 cut(s) 864, 898
BciVI GTATCC 1 cut(s) 109
BcnI CCSGG 2 cut(s) 2757, 2838
BcoDI GTCTC 7 cut(s) 260, 279, 891, 1715, 1740, 3533, 3573
BcuI ACTAGT 1 cut(s) 3464
BfaI CTAG 7 cut(s) 894, 1739, 1983, 2958, 3351, 3465, 3585
BfmI CTRYAG 7 cut(s) 873, 1613, 2360, 2459, 2799, 3357, 3398
BfoI RGCGCY 2 cut(s) 1954, 3002
BfuAI ACCTGC 2 cut(s) 2356, 3317
BfuI GTATCC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 2089
BglII AGATCT 1 cut(s) 1609
BlnI CCTAGG 1 cut(s) 3584
BlpI GCTNAGC 1 cut(s) 1302
Bme18I GGWCC 4 cut(s) 2368, 3094, 3158, 3794
BmeRI GACNNNNNGTC 2 cut(s) 3390, 3553
BmgT120I GGNCC 6 cut(s) 330, 2368, 2778, 3094, 3158, 3794
BmsI GCATC 9 cut(s) 40, 139, 509, 1336, 1640, 2571, 2589, 3287, 3727
BmtI GCTAGC 1 cut(s) 2961
BoxI GACNNNNGTC 2 cut(s) 1498, 2396
BpiI GAAGAC 3 cut(s) 890, 990, 2253
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 2 cut(s) 1967, 3572
Bpu10I CCTNAGC 1 cut(s) 3090
Bpu1102I GCTNAGC 1 cut(s) 1302
BpuEI CTTGAG 6 cut(s) 12, 152, 1390, 1937, 2130, 2537
BpuMI CCSGG 2 cut(s) 2757, 2838
BsaBI GATNNNNATC 1 cut(s) 3678
BsaHI GRCGYC 1 cut(s) 1724
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 9 cut(s) 601, 1384, 1449, 2060, 2087, 2756, 3118, 3119, 3584
BsaWI WCCGGW 1 cut(s) 143
BsaXI ACNNNNNCTCC 8 cut(s) 257, 287, 369, 399, 502, 532, 3078, 3108
Bse1I ACTGG 6 cut(s) 220, 395, 1366, 1402, 1762, 3097
Bse21I CCTNAGG 1 cut(s) 1509
Bse3DI GCAATG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 3678
BseDI CCNNGG 9 cut(s) 601, 1384, 1449, 2060, 2087, 2756, 3118, 3119, 3584
BseGI GGATG 4 cut(s) 55, 768, 2766, 2973
BseJI GATNNNNATC 1 cut(s) 3678
BseMI GCAATG 1 cut(s) 51
BseNI ACTGG 6 cut(s) 220, 395, 1366, 1402, 1762, 3097
BseRI GAGGAG 4 cut(s) 15, 79, 1802, 2880
BseSI GKGCMC 1 cut(s) 1346
BseYI CCCAGC 1 cut(s) 3050
BsgI GTGCAG 3 cut(s) 899, 1416, 1539
Bsh1236I CGCG 1 cut(s) 88
BshFI GGCC 7 cut(s) 22, 331, 600, 2755, 2780, 2805, 3583
BshNI GGYRCC 1 cut(s) 3113
BsiHKAI GWGCWC 3 cut(s) 2407, 3091, 3731
BsiSI CCGG 3 cut(s) 144, 2756, 2837
BslFI GGGAC 8 cut(s) 580, 1163, 1486, 2354, 2924, 3085, 3454, 3738
BsmAI GTCTC 7 cut(s) 260, 279, 891, 1715, 1740, 3533, 3573
BsmBI CGTCTC 4 cut(s) 891, 1715, 1740, 3573
BsmFI GGGAC 8 cut(s) 580, 1163, 1486, 2354, 2924, 3085, 3454, 3738
BsmI GAATGC 3 cut(s) 955, 2431, 2905
BsnI GGCC 7 cut(s) 22, 331, 600, 2755, 2780, 2805, 3583
Bso31I GGTCTC 1 cut(s) 260
Bsp1286I GDGCHC 7 cut(s) 510, 1346, 1714, 2381, 2407, 3091, 3731
Bsp1720I GCTNAGC 1 cut(s) 1302
BspANI GGCC 7 cut(s) 22, 331, 600, 2755, 2780, 2805, 3583
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 4 cut(s) 738, 1812, 2158, 3421
BspMAI CTGCAG 5 cut(s) 877, 1617, 2364, 2463, 3361
BspMI ACCTGC 2 cut(s) 2356, 3317
BspOI GCTAGC 1 cut(s) 2961
BspT107I GGYRCC 1 cut(s) 3113
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 6 cut(s) 220, 395, 1366, 1402, 1762, 3097
BssECI CCNNGG 9 cut(s) 601, 1384, 1449, 2060, 2087, 2756, 3118, 3119, 3584
BssNI GRCGYC 1 cut(s) 1724
BssT1I CCWWGG 3 cut(s) 1384, 1449, 3584
Bst4CI ACNGT 9 cut(s) 381, 723, 996, 1338, 1963, 2691, 2898, 3079, 3652
Bst6I CTCTTC 2 cut(s) 119, 937
BstACI GRCGYC 1 cut(s) 1724
BstAPI GCANNNNNTGC 5 cut(s) 50, 2119, 2599, 2680, 3275
BstENI CCTNNNNNAGG 3 cut(s) 27, 339, 3012
BstF5I GGATG 4 cut(s) 55, 768, 2766, 2973
BstFNI CGCG 1 cut(s) 88
BstH2I RGCGCY 2 cut(s) 1954, 3002
BstHHI GCGC 2 cut(s) 1953, 3001
BstMAI GTCTC 7 cut(s) 260, 279, 891, 1715, 1740, 3533, 3573
BstNSI RCATGY 4 cut(s) 1156, 1414, 1441, 2458
BstPAI GACNNNNGTC 2 cut(s) 1498, 2396
BstSFI CTRYAG 7 cut(s) 873, 1613, 2360, 2459, 2799, 3357, 3398
BstSLI GKGCMC 1 cut(s) 1346
BstUI CGCG 1 cut(s) 88
BstV2I GAAGAC 3 cut(s) 890, 990, 2253
BstX2I RGATCY 9 cut(s) 344, 1609, 1623, 1649, 1678, 2329, 2840, 3347, 3370
BstYI RGATCY 9 cut(s) 344, 1609, 1623, 1649, 1678, 2329, 2840, 3347, 3370
Bsu36I CCTNAGG 1 cut(s) 1509
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 7 cut(s) 22, 331, 600, 2755, 2780, 2805, 3583
BtsCI GGATG 4 cut(s) 55, 768, 2766, 2973
BtsI GCAGTG 2 cut(s) 882, 2357
BtsIMutI CAGTG 8 cut(s) 402, 882, 2348, 2357, 2407, 2734, 3292, 3670
BveI ACCTGC 2 cut(s) 2356, 3317
CaiI CAGNNNCTG 1 cut(s) 2680
CciI TCATGA 4 cut(s) 738, 1812, 2158, 3421
CfoI GCGC 2 cut(s) 1953, 3001
Cfr13I GGNCC 6 cut(s) 330, 2368, 2778, 3094, 3158, 3794
CsiI ACCWGGT 2 cut(s) 1993, 3337
Csp6I GTAC 5 cut(s) 524, 712, 1389, 1825, 1849
CviQI GTAC 5 cut(s) 524, 712, 1389, 1825, 1849
DriI GACNNNNNGTC 2 cut(s) 3390, 3553
EaeI YGGCCR 1 cut(s) 598
Eam1104I CTCTTC 2 cut(s) 119, 937
Eam1105I GACNNNNNGTC 2 cut(s) 3390, 3553
EarI CTCTTC 2 cut(s) 119, 937
EciI GGCGGA 1 cut(s) 450
Ecl136II GAGCTC 1 cut(s) 3089
Eco130I CCWWGG 3 cut(s) 1384, 1449, 3584
Eco147I AGGCCT 2 cut(s) 22, 3583
Eco24I GRGCYC 4 cut(s) 510, 1714, 2381, 3091
Eco31I GGTCTC 1 cut(s) 260
Eco32I GATATC 2 cut(s) 3126, 3661
Eco47I GGWCC 4 cut(s) 2368, 3094, 3158, 3794
Eco53kI GAGCTC 1 cut(s) 3089
Eco57I CTGAAG 7 cut(s) 884, 932, 1946, 2465, 2861, 3720, 3762
Eco81I CCTNAGG 1 cut(s) 1509
EcoICRI GAGCTC 1 cut(s) 3089
EcoNI CCTNNNNNAGG 3 cut(s) 27, 339, 3012
EcoO109I RGGNCCY 3 cut(s) 330, 2368, 2778
EcoRI GAATTC 2 cut(s) 577, 694
EcoRV GATATC 2 cut(s) 3126, 3661
EcoT14I CCWWGG 3 cut(s) 1384, 1449, 3584
EcoT22I ATGCAT 1 cut(s) 1329
EcoT38I GRGCYC 4 cut(s) 510, 1714, 2381, 3091
ErhI CCWWGG 3 cut(s) 1384, 1449, 3584
Esp3I CGTCTC 4 cut(s) 891, 1715, 1740, 3573
FalI AAGNNNNNCTT 4 cut(s) 930, 962, 2000, 2032
FaqI GGGAC 8 cut(s) 580, 1163, 1486, 2354, 2924, 3085, 3454, 3738
FauNDI CATATG 2 cut(s) 789, 2109
FblI GTMKAC 1 cut(s) 3397
FokI GGATG 4 cut(s) 62, 755, 2773, 2960
FriOI GRGCYC 4 cut(s) 510, 1714, 2381, 3091
FspBI CTAG 7 cut(s) 894, 1739, 1983, 2958, 3351, 3465, 3585
GlaI GCGC 2 cut(s) 1952, 3000
GsaI CCCAGC 1 cut(s) 3054
GsuI CTGGAG 2 cut(s) 1967, 3572
HaeII RGCGCY 2 cut(s) 1954, 3002
HaeIII GGCC 7 cut(s) 22, 331, 600, 2755, 2780, 2805, 3583
HapII CCGG 3 cut(s) 144, 2756, 2837
HhaI GCGC 2 cut(s) 1953, 3001
Hin1I GRCGYC 1 cut(s) 1724
Hin6I GCGC 2 cut(s) 1951, 2999
HinP1I GCGC 2 cut(s) 1951, 2999
HincII GTYRAC 3 cut(s) 2923, 3528, 3558
HindII GTYRAC 3 cut(s) 2923, 3528, 3558
HpaII CCGG 3 cut(s) 144, 2756, 2837
HphI GGTGA 8 cut(s) 502, 822, 2711, 2955, 2997, 3252, 3377, 3719
Hpy166II GTNNAC 4 cut(s) 2923, 3398, 3528, 3558
Hpy8I GTNNAC 4 cut(s) 2923, 3398, 3528, 3558
HpyCH4III ACNGT 9 cut(s) 381, 723, 996, 1338, 1963, 2691, 2898, 3079, 3652
HpyCH4IV ACGT 3 cut(s) 1724, 1733, 3566
HpySE526I ACGT 3 cut(s) 1724, 1733, 3566
Hsp92I GRCGYC 1 cut(s) 1724
HspAI GCGC 2 cut(s) 1951, 2999
LmnI GCTCC 5 cut(s) 66, 1948, 2100, 2410, 3086
LweI GCATC 9 cut(s) 40, 139, 509, 1336, 1640, 2571, 2589, 3287, 3727
MabI ACCWGGT 2 cut(s) 1993, 3337
MaeI CTAG 7 cut(s) 894, 1739, 1983, 2958, 3351, 3465, 3585
MaeII ACGT 3 cut(s) 1724, 1733, 3566
MfeI CAATTG 3 cut(s) 285, 2180, 2534
MflI RGATCY 9 cut(s) 344, 1609, 1623, 1649, 1678, 2329, 2840, 3347, 3370
MhlI GDGCHC 7 cut(s) 510, 1346, 1714, 2381, 2407, 3091, 3731
MlyI GAGTC 5 cut(s) 511, 1480, 1877, 2386, 3563
MmeI TCCRAC 6 cut(s) 204, 517, 796, 2410, 3040, 3064
Mph1103I ATGCAT 1 cut(s) 1329
MslI CAYNNNNRTG 2 cut(s) 1767, 3177
MspA1I CMGCKG 1 cut(s) 2680
MspI CCGG 3 cut(s) 144, 2756, 2837
MunI CAATTG 3 cut(s) 285, 2180, 2534
Mva1269I GAATGC 3 cut(s) 955, 2431, 2905
MvnI CGCG 1 cut(s) 88
NciI CCSGG 2 cut(s) 2757, 2838
NdeI CATATG 2 cut(s) 789, 2109
NheI GCTAGC 1 cut(s) 2957
NmuCI GTSAC 5 cut(s) 381, 1515, 2127, 2898, 3287
NsiI ATGCAT 1 cut(s) 1329
NspI RCATGY 4 cut(s) 1156, 1414, 1441, 2458
PaeI GCATGC 1 cut(s) 1414
PagI TCATGA 4 cut(s) 738, 1812, 2158, 3421
PasI CCCWGGG 1 cut(s) 3119
PceI AGGCCT 2 cut(s) 22, 3583
PcsI WCGNNNNNNNCGW 1 cut(s) 1730
PctI GAATGC 3 cut(s) 955, 2431, 2905
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 2 cut(s) 1297, 3344
PfoI TCCNGGA 2 cut(s) 340, 2332
PleI GAGTC 5 cut(s) 511, 1479, 1877, 2386, 3562
PpsI GAGTC 5 cut(s) 511, 1479, 1877, 2386, 3562
PpuMI RGGWCCY 1 cut(s) 2368
PshAI GACNNNNGTC 2 cut(s) 1498, 2396
PshBI ATTAAT 3 cut(s) 1295, 2046, 2472
Psp124BI GAGCTC 1 cut(s) 3091
Psp5II RGGWCCY 1 cut(s) 2368
PspFI CCCAGC 1 cut(s) 3050
PspPI GGNCC 6 cut(s) 330, 2368, 2778, 3094, 3158, 3794
PspPPI RGGWCCY 1 cut(s) 2368
PstI CTGCAG 5 cut(s) 877, 1617, 2364, 2463, 3361
PstNI CAGNNNCTG 1 cut(s) 2680
PsuI RGATCY 9 cut(s) 344, 1609, 1623, 1649, 1678, 2329, 2840, 3347, 3370
PsyI GACNNNGTC 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 2680
RsaI GTAC 5 cut(s) 525, 713, 1390, 1826, 1850
RsaNI GTAC 5 cut(s) 524, 712, 1389, 1825, 1849
RseI CAYNNNNRTG 2 cut(s) 1767, 3177
SacI GAGCTC 1 cut(s) 3091
Sau96I GGNCC 6 cut(s) 330, 2368, 2778, 3094, 3158, 3794
SchI GAGTC 5 cut(s) 511, 1480, 1877, 2386, 3563
SduI GDGCHC 7 cut(s) 510, 1346, 1714, 2381, 2407, 3091, 3731
SexAI ACCWGGT 2 cut(s) 1993, 3337
SfaNI GCATC 9 cut(s) 40, 139, 509, 1336, 1640, 2571, 2589, 3287, 3727
SfcI CTRYAG 7 cut(s) 873, 1613, 2360, 2459, 2799, 3357, 3398
SinI GGWCC 4 cut(s) 2368, 3094, 3158, 3794
SmiMI CAYNNNNRTG 2 cut(s) 1767, 3177
SmlI CTYRAG 6 cut(s) 27, 167, 1405, 1916, 2145, 2552
SmoI CTYRAG 6 cut(s) 27, 167, 1405, 1916, 2145, 2552
SpeI ACTAGT 1 cut(s) 3464
SphI GCATGC 1 cut(s) 1414
SseBI AGGCCT 2 cut(s) 22, 3583
SspMI CTAG 7 cut(s) 894, 1739, 1983, 2958, 3351, 3465, 3585
SstI GAGCTC 1 cut(s) 3091
StuI AGGCCT 2 cut(s) 22, 3583
StyI CCWWGG 3 cut(s) 1384, 1449, 3584
TaaI ACNGT 9 cut(s) 381, 723, 996, 1338, 1963, 2691, 2898, 3079, 3652
TaiI ACGT 3 cut(s) 1727, 1736, 3569
TaqII GACCGA 1 cut(s) 3146
TatI WGTACW 1 cut(s) 1824
TauI GCSGC 6 cut(s) 91, 976, 1114, 2086, 3640, 3692
TscAI CASTG 8 cut(s) 402, 882, 2355, 2364, 2407, 2734, 3292, 3670
TseFI GTSAC 5 cut(s) 381, 1515, 2127, 2898, 3287
Tsp45I GTSAC 5 cut(s) 381, 1515, 2127, 2898, 3287
TspGWI ACGGA 2 cut(s) 250, 2295
TspRI CASTG 8 cut(s) 402, 882, 2355, 2364, 2407, 2734, 3292, 3670
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 2 cut(s) 1297, 3344
VpaK11BI GGWCC 4 cut(s) 2368, 3094, 3158, 3794
VspI ATTAAT 3 cut(s) 1295, 2046, 2472
XagI CCTNNNNNAGG 3 cut(s) 27, 339, 3012
XbaI TCTAGA 2 cut(s) 1738, 3350
XceI RCATGY 4 cut(s) 1156, 1414, 1441, 2458
XmaJI CCTAGG 1 cut(s) 3584
XmiI GTMKAC 1 cut(s) 3397
XspI CTAG 7 cut(s) 894, 1739, 1983, 2958, 3351, 3465, 3585
ZraI GACGTC 1 cut(s) 1725
Zsp2I ATGCAT 1 cut(s) 1329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.