MD11G1036600.v1.1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006832
Physical Location & Seq
Reverse (-)
3162147 .. 3171338
9192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1036600.v1.1.491

Sequence Viewer

Length: 4173 bp
ATGGATACCAACTGGAGGCCTCCTCAAGGTGGAGAGCCTGCCATGGATGCCGGTGATTGGAGGAGTCAGCTGCAGGCTGATTCAAGGCAAAGAATTGTCAACAAGATAATGGATACGTTGAAGAGGCATCTTCCCTTCTCCGGACAGGAGGGATTACTGGAACTCAAGAAAATTGCTGTAAGGTTTGAGGAAAAGATTTATACTGCAGCCACAAGCCAGTCGGATTATCTACGGAAAATTTCGCTGAAGATGCTCACAATGGAGACCAAGTCTCAGAATTCTACAATGGCCAATTCCTTACAATCTAACTCTGCTGGTAACAGCAACAGGCCTCTGGATCCAGGGTCTTTTGCTATGCAACCCCAAGTCCCCAATCAAGGGCAATCACTCTCTATGCCGTTGCCAGCTAATCAATCACAAGGACGCCAACAACTTTTATCTCAGAACATTCAGAATAATGTTCCGCCTGCTGGAGTTCAAAGTTCGTCTGGCTTATCATCTGCACTACCTCCTTCTTCCGGTTCAACCCAGACTCCTATCCCAAGTATTGTTGGACAAAATCCGAACATGCAAAATATGTCTGGAAGTTCACAGAACTCATTAGGGAACTCCATGGGCCAGGGGGTACCCTCCAATATGTTTGCTAATTCAAGGCAAATGCCAGGAAGGCAACAGGTAGTTCCCCAACAGCAGCAGCCGCAGCAATCCCAGAATTCGCAGCAATATATGTACCCTCAACAGCTGCAACATTCGCTTTTGAAGCCCAAATATCAGCAGGGAAATATGCCACAACTTGTGCAACAGCAACAGCAGCAAAACCTATTACAACCGACTCAGCTGCAGTCTTCACAGCAATCTGTTATGCCGACATCATCTGTTATGCAGCCTTCGGTTCAACCATCGCTCTCGAGTCTTCAGCAGAATCAACAATCTGGAATGCAACAGTCATCACAACCCATGCTTCAGCAGCATTCACAGCCAGTTCTCAGGCAGCAACAGCAGCCTCAACAGGCCGCTGTTCATCAACAGCAAACACCAATGCCACAACAGCCAATATTACCCCAGCAACAGCAACAGCAGCAGCAGCAGCAGCAGCAGCCGCTTATGGTGCAACAGACAACTGCTTCAAACTTGTCCCAGAATCAGATGATTGGACAACAAAACAATGTGGGTGATATGCAGCAGCAGCAACAACAGAGGTTGCTAAGCCAAAAGAATAGTCTTTTAAACCTGCAACAACAACAGCAGCAGCAGCAACAGCAACAGCAAAAGCAACAACAACAGCAGTTAATGGCTCAACAAAGCAACCTTTCTAATATGCATCAGCAACAATTGGGCCCTCAGAGTAATGTTACCGGGTTACAGCAACAGCAGCAAAGCAACCTTTCAAATATGCATCAGCAACAGTTGGGCTCCCAGAGTAATGTTACTGGATTACAGCAACAGCAGCACCTCGGAACTCAAAGTGGTAATTCTAGCATGCAACCAAATCAGCACTCTGTACACTTGTTGCAACAATCCAAGGTTCAAGTGCAGCAACAACAACATCAGAATGCATCTAACATGTTACCTTCTCAAGGACAGCAGTCACAGCCTCAGGCATCACAGCAACAAATGATGTCACAGATTCAATCACAGCCTTCCCAGATGCAACAAATGAGTTTGCAACAGCAGTCAAATCCACTGCAACGAGATATGCAACAAAGACTTCAAGCATCAGGTCAGATATCAGGAACCATGCTTCAACCACAAAACGTAATGGATCAGCAAAAGCAGTTATATCAAAATCAAACTCAAAGAGCCCATCCTGAAACATCATCGACATCTCTAGATTCCACAGCTCAGACTGGACATGCAACTGGGGGTGATTGGCAAGAGGAGGTCTATCAAAAAATCAAGAGCATGAAGGAAATGTACTTCCCCGAACTAAGTGAAATGTATCAGAAAATTGCTACTAAACTTCAGCAGCACGATTCTCTTCCACAACAACCAAAGTTAGAGCAGCTTGAGAAGCTAAAAATGTTCAAAACCATGTTGGAGCGCCTCATATCAATCTTACAGATTCCCAAGAGTAGCATTACATCTGGTTTGAAAGAGAAGTTGGGTACATATGAGAAGCAGATAGTAAATTTTATTAATACAAATAGACCGAGGAAGCAAGTTCCTCCTCTGCAACAAGGGCAGCTCCCCCCACCTCATATGCACTCCATGCAGCAGCCGCAATCTCAAATTACTCAAGTGCAGTCGCATGAAAATCAAATGAACCCGCAGTTGCAAACAATGAATTTACAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACAAGCTTGCAGCAAAATTCGATGTCTTCTATATCTGGGGTTTCAACAGCACAGCAGAACATGATGAATTCATTGCAGCCGAGTACAAATTTGGATTCAGGACAGGGAAATGCACTGAACTCTTTGCAGCAAGTTCCGGTGGGATCTATCCAACAAACTCCTGTCAGTGCTCCCCAGCAAGCAAACATGAATGCCTTGTCATCACAGAGTGGGGTTAGTATGCTGCAGCCAAATATTAATTCCCTCCAGTCAAATTCTGGTATGCTTCAACACCAGCAAATAAAACAGCAGGAACAGCAAATGTTTCATAATCAACTGAAGCAACAGTATCAACAACAGCGATTGCAGCAGCAATTGATTCAGAAGCAGCAGATGCTGCAACAGCAGCAGCAGCAGCAACACTTGCAGCAAGCAAAGCAACAGCTTCCAGCACAGATGCAGGTGCACCAACACCAAATGCCACAGCTTCATCAAATGAATGATGTAAATGACTTGAAGATGAGACAGGGGATGGGTGTTAAGCCAGGGGTTTTTCAGCAACATATGCCTGCAGGCCAGCGTGCTTATCCCCAACAGTTGAAATCAGGATCTCCATTTCCTATTCCATCAACAAACCAACTCCTTCAGGCTGCATCTCCTCAAATTTCACAACATTCTTCTCCCCAAGTTGACCAGCAGAATATACTGACTCACCCGAAAGTTGGAACCCCTTTGCAAACTGCTGGTTCACCTTTTGTCATCCCATCTCCATCAACTCCCATGGCCCCATCCCCCATGCCAGGGGATTCTGAGAAACCTTCCTCACTATTGAATGCTGGGAATATTGGACATCAACAAGCAGCTGGGGTGGGAGCACCGGTCCAGTCCCTTGCAATTGGCACCCCTGGGATATCAGCCTCTCCTTTGCTTGCTGAATTTAGTGTACCAGACGCTACTCATGTTAATGCTTTGTCAACTATTTCTGGCAAGTCGAGTGTTACCGAGCAGCCTCTTGAACGCTTGATTAAGGCGGTGAAATCGATGTCGCCTAATGCATTGAGTGCATCTGTCAGTGACATTGGCTCAGTGGTTAGTATGATTGATAGGATAGCAGGGTCAGCCCCAGGTAATGGATCTAGAGCTGCAGTTGGTGAGGATTTGGTTGCAATGACAAAATGTCGTCTGCAAGCAAGAAACGTTATGACTCAGGATGGATCAAATGGGACTAGGAAGATGAGGCGCTACACTAGTGCCATGCCCTTAAATGTTGTGTCTTCTGCTGGTAGTATGAATGATAGTTTCAAGCAGTTGATCAATTCAGAGACATCTGACCTGGAGTCAACTGCAACATCTCGTATTAAGAGGCCTAGGGTTGAGGCTAATCATGCTCTTCTGGAAGAAATAAGAGAAATAAATAGGCGGCTTATCGACACAGTTGTTGATATCAGTGATGAAGATGTTGACCCTAGTGCAGCTGCTGCCGAAGGGGGTGAGGGTACTGTTGTCAAGTGCTCTTTTGATGCCGTGGCTCTCAGTCCAAGCTTGAAATCGCAGTATGCTTCAGCACAAATGTCACCAATTCAGCCATTAAGACTGCTTGTTCCTACAAATTATCCCAACTGCTCTCCGGTTCTCTTGGACAAGTTTCCAGTTGAAGTCAGTAAGGAGTGTGAAGATCTTTCTGTGAAGGCCAAGTCGAGGTTTAGCATATCTCTAAGAAGCATTTCACAACCCATGTCCCTTGGGGAGATAGCAAGGACTTGGGATGTTTGTGCACGTGCAGTTATTTCTGAGCATGCGCAGCAGAGCGGTGGAGGAAGTTTCAGCTCAAAGTACGGGACATGGGAGAACTGCTTGAGCGCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1391

Amino Acids

153.38

Weight (kDa)

9.41

Isoelectric Point (pI)

77.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 18 - 96 1.9e-34 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2755
Acc16I TGCGCA 1 cut(s) 4104
Acc36I ACCTGC 2 cut(s) 1239, 2755
Acc65I GGTACC 1 cut(s) 625
AccB1I GGYRCC 2 cut(s) 625, 3203
AccB7I CCANNNNNTGG 1 cut(s) 3434
AccBSI CCGCTC 1 cut(s) 4113
AccIII TCCGGA 1 cut(s) 140
AciI CCGC 9 cut(s) 464, 698, 1014, 1100, 2219, 2267, 3335, 3724, 4113
AclI AACGTT 1 cut(s) 3501
AclWI GGATC 7 cut(s) 332, 345, 1770, 2476, 2920, 3445, 3526
AcoI YGGCCR 1 cut(s) 288
AcuI CTGAAG 7 cut(s) 266, 899, 947, 1946, 2663, 2933, 3849
AcvI CACGTG 1 cut(s) 4082
AcyI GRCGYC 1 cut(s) 424
AdeI CACNNNGTG 1 cut(s) 2534
AfaI GTAC 9 cut(s) 627, 731, 1503, 1916, 2107, 2410, 3249, 3802, 4139
AfeI AGCGCT 1 cut(s) 4165
AflIII ACRYGT 1 cut(s) 1563
AgeI ACCGGT 1 cut(s) 3181
AhdI GACNNNNNGTC 2 cut(s) 3480, 3640
AhlI ACTAGT 1 cut(s) 3551
AjnI CCWGG 8 cut(s) 340, 618, 661, 2848, 3103, 3208, 3427, 3636
AjuI GAANNNNNNNTTGG 4 cut(s) 2084, 2116, 2548, 2580
Alw21I GWGCWC 5 cut(s) 2497, 2772, 3181, 3818, 4081
Alw26I GTCTC 4 cut(s) 257, 276, 2821, 3620
Alw44I GTGCAC 2 cut(s) 2768, 4077
AlwI GGATC 7 cut(s) 332, 345, 1770, 2476, 2920, 3445, 3526
AlwNI CAGNNNCTG 2 cut(s) 2701, 3782
Ama87I CYCGRG 1 cut(s) 907
Aor13HI TCCGGA 1 cut(s) 140
Aor51HI AGCGCT 1 cut(s) 4165
ApaI GGGCCC 1 cut(s) 1340
ApaLI GTGCAC 2 cut(s) 2768, 4077
ArsI GACNNNNNNTTYG 2 cut(s) 1204, 1236
AseI ATTAAT 2 cut(s) 2136, 2562
AsiGI ACCGGT 1 cut(s) 3181
Asp700I GAANNNNTTC 1 cut(s) 4027
Asp718I GGTACC 1 cut(s) 625
AspA2I CCTAGG 1 cut(s) 3671
AspLEI GCGC 4 cut(s) 2043, 3546, 4105, 4166
AspS9I GGNCC 5 cut(s) 616, 1336, 1337, 3088, 3184
AsuC2I CCSGG 1 cut(s) 1357
AsuHPI GGTGA 9 cut(s) 65, 1184, 1877, 3008, 3045, 3349, 3467, 3806, 3870
AvaI CYCGRG 1 cut(s) 907
AvaII GGWCC 1 cut(s) 3184
AvrII CCTAGG 1 cut(s) 3671
AxyI CCTNAGG 1 cut(s) 1596
BaeGI GKGCMC 3 cut(s) 1340, 2772, 4081
BaeI ACNNNNGTAYC 2 cut(s) 3792, 3825
BalI TGGCCA 1 cut(s) 290
BamHI GGATCC 1 cut(s) 337
BanI GGYRCC 2 cut(s) 625, 3203
BanII GRGCYC 3 cut(s) 1340, 1415, 1804
BarI GAAGNNNNNNTAC 2 cut(s) 1898, 1930
BbrPI CACGTG 1 cut(s) 4082
BbsI GAAGAC 4 cut(s) 837, 905, 2343, 3570
Bbv12I GWGCWC 5 cut(s) 2497, 2772, 3181, 3818, 4081
BccI CCATC 8 cut(s) 907, 1812, 2830, 2938, 3076, 3082, 3100, 3509
BceAI ACGGC 2 cut(s) 382, 3812
BcgI CGANNNNNNTGC 2 cut(s) 820, 854
BciT130I CCWGG 8 cut(s) 342, 620, 663, 2850, 3105, 3210, 3429, 3638
BciVI GTATCC 1 cut(s) 106
BclI TGATCA 1 cut(s) 3615
BcnI CCSGG 1 cut(s) 1357
BcoDI GTCTC 4 cut(s) 257, 276, 2821, 3620
BcuI ACTAGT 1 cut(s) 3551
BfaI CTAG 7 cut(s) 1476, 1829, 3441, 3531, 3552, 3672, 3771
BfmI CTRYAG 6 cut(s) 71, 204, 839, 2549, 2874, 3447
BfoI RGCGCY 3 cut(s) 2044, 3547, 4167
BfuAI ACCTGC 2 cut(s) 1239, 2755
BfuI GTATCC 1 cut(s) 106
BglI GCCNNNNNGGC 1 cut(s) 667
BglII AGATCT 1 cut(s) 3979
BlnI CCTAGG 1 cut(s) 3671
BlpI GCTNAGC 1 cut(s) 1205
Bme1390I CCNGG 9 cut(s) 342, 620, 663, 1357, 2850, 3105, 3210, 3429, 3638
Bme18I GGWCC 1 cut(s) 3184
BmeRI GACNNNNNGTC 2 cut(s) 3480, 3640
BmeT110I CYCGRG 1 cut(s) 907
BmgT120I GGNCC 5 cut(s) 616, 1336, 1337, 3088, 3184
BmiI GGNNCC 8 cut(s) 339, 627, 1338, 1414, 1735, 3031, 3090, 3205
BmrFI CCNGG 9 cut(s) 342, 620, 663, 1357, 2850, 3105, 3210, 3429, 3638
BmrI ACTGGG 1 cut(s) 1869
BmuI ACTGGG 1 cut(s) 1869
BoxI GACNNNNGTC 1 cut(s) 1585
BpiI GAAGAC 4 cut(s) 837, 905, 2343, 3570
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 4 cut(s) 34, 492, 2555, 3659
Bpu1102I GCTNAGC 1 cut(s) 1205
BpuEI CTTGAG 5 cut(s) 9, 149, 1560, 2027, 2220
BpuMI CCSGG 1 cut(s) 1357
Bsa29I ATCGAT 1 cut(s) 3344
BsaAI YACGTR 1 cut(s) 4082
BsaHI GRCGYC 1 cut(s) 424
BsaI GGTCTC 1 cut(s) 257
BsaWI WCCGGW 5 cut(s) 140, 518, 2461, 3181, 3931
BsaXI ACNNNNNCTCC 6 cut(s) 254, 284, 517, 547, 3168, 3198
Bse118I RCCGGY 2 cut(s) 50, 3181
Bse21I CCTNAGG 1 cut(s) 1596
Bse3DI GCAATG 2 cut(s) 2396, 3477
BseAI TCCGGA 1 cut(s) 140
BseBI CCWGG 8 cut(s) 342, 620, 663, 2850, 3105, 3210, 3429, 3638
BseCI ATCGAT 1 cut(s) 3344
BseGI GGATG 7 cut(s) 52, 1804, 2841, 3063, 3092, 3520, 4075
BseMI GCAATG 2 cut(s) 2396, 3477
BseRI GAGGAG 5 cut(s) 12, 76, 1892, 2157, 2952
BseSI GKGCMC 3 cut(s) 1340, 2772, 4081
BseYI CCCAGC 4 cut(s) 1062, 2499, 3140, 3167
BsgI GTGCAG 5 cut(s) 486, 1553, 2261, 3795, 4104
BshNI GGYRCC 2 cut(s) 625, 3203
BshTI ACCGGT 1 cut(s) 3181
BshVI ATCGAT 1 cut(s) 3344
BsiHKAI GWGCWC 5 cut(s) 2497, 2772, 3181, 3818, 4081
BsiHKCI CYCGRG 1 cut(s) 907
BsiSI CCGG 7 cut(s) 51, 141, 519, 1356, 2462, 3182, 3932
BslFI GGGAC 6 cut(s) 353, 1120, 3175, 3541, 4027, 4156
BsmAI GTCTC 4 cut(s) 257, 276, 2821, 3620
BsmFI GGGAC 6 cut(s) 353, 1120, 3175, 3541, 4027, 4156
BsmI GAATGC 5 cut(s) 942, 970, 1558, 2521, 3142
Bso31I GGTCTC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 907
Bsp120I GGGCCC 1 cut(s) 1336
Bsp1286I GDGCHC 8 cut(s) 1340, 1415, 1804, 2497, 2772, 3181, 3818, 4081
Bsp13I TCCGGA 1 cut(s) 140
Bsp1407I TGTACA 1 cut(s) 1501
Bsp143I GATC 8 cut(s) 337, 1762, 2468, 2912, 3437, 3518, 3615, 3979
Bsp1720I GCTNAGC 1 cut(s) 1205
Bsp19I CCATGG 3 cut(s) 42, 612, 3084
BspACI CCGC 9 cut(s) 464, 698, 1014, 1100, 2219, 2267, 3335, 3724, 4113
BspDI ATCGAT 1 cut(s) 3344
BspEI TCCGGA 1 cut(s) 140
BspLI GGNNCC 8 cut(s) 339, 627, 1338, 1414, 1735, 3031, 3090, 3205
BspMAI CTGCAG 6 cut(s) 75, 208, 843, 2553, 2878, 3451
BspMI ACCTGC 2 cut(s) 1239, 2755
BspPI GGATC 7 cut(s) 332, 345, 1770, 2476, 2920, 3445, 3526
BspQI GCTCTTC 1 cut(s) 3699
BspT107I GGYRCC 2 cut(s) 625, 3203
BspTNI GGTCTC 1 cut(s) 257
BsrBI CCGCTC 1 cut(s) 4113
BsrDI GCAATG 2 cut(s) 2396, 3477
BsrFI RCCGGY 2 cut(s) 50, 3181
BsrGI TGTACA 1 cut(s) 1501
BssAI RCCGGY 2 cut(s) 50, 3181
BssMI GATC 8 cut(s) 337, 1762, 2468, 2912, 3437, 3518, 3615, 3979
BssNI GRCGYC 1 cut(s) 424
BssT1I CCWWGG 6 cut(s) 42, 612, 1521, 3084, 3671, 4045
Bst2UI CCWGG 8 cut(s) 342, 620, 663, 2850, 3105, 3210, 3429, 3638
Bst4CI ACNGT 6 cut(s) 945, 1407, 2652, 2901, 3739, 3805
Bst6I CTCTTC 3 cut(s) 116, 1983, 3699
BstACI GRCGYC 1 cut(s) 424
BstAPI GCANNNNNTGC 7 cut(s) 2209, 2698, 2701, 2728, 2869, 3365, 3782
BstAUI TGTACA 1 cut(s) 1501
BstBAI YACGTR 1 cut(s) 4082
BstDSI CCRYGG 4 cut(s) 42, 612, 3084, 3828
BstENI CCTNNNNNAGG 2 cut(s) 24, 1575
BstF5I GGATG 7 cut(s) 52, 1804, 2841, 3063, 3092, 3520, 4075
BstH2I RGCGCY 3 cut(s) 2044, 3547, 4167
BstHHI GCGC 4 cut(s) 2043, 3546, 4105, 4166
BstKTI GATC 8 cut(s) 340, 1765, 2471, 2915, 3440, 3521, 3618, 3982
BstMAI GTCTC 4 cut(s) 257, 276, 2821, 3620
BstMBI GATC 8 cut(s) 337, 1762, 2468, 2912, 3437, 3518, 3615, 3979
BstNI CCWGG 8 cut(s) 342, 620, 663, 2850, 3105, 3210, 3429, 3638
BstNSI RCATGY 5 cut(s) 571, 1483, 1567, 1856, 4103
BstPAI GACNNNNGTC 1 cut(s) 1585
BstSCI CCNGG 9 cut(s) 340, 618, 661, 1355, 2848, 3103, 3208, 3427, 3636
BstSFI CTRYAG 6 cut(s) 71, 204, 839, 2549, 2874, 3447
BstSLI GKGCMC 3 cut(s) 1340, 2772, 4081
BstV2I GAAGAC 4 cut(s) 837, 905, 2343, 3570
BstX2I RGATCY 5 cut(s) 337, 2468, 2912, 3437, 3979
BstYI RGATCY 5 cut(s) 337, 2468, 2912, 3437, 3979
Bsu15I ATCGAT 1 cut(s) 3344
Bsu36I CCTNAGG 1 cut(s) 1596
BsuI GTATCC 1 cut(s) 106
BsuTUI ATCGAT 1 cut(s) 3344
BtgI CCRYGG 4 cut(s) 42, 612, 3084, 3828
BtgZI GCGATG 1 cut(s) 885
BtsCI GGATG 7 cut(s) 52, 1804, 2841, 3063, 3092, 3520, 4075
BtsI GCAGTG 1 cut(s) 1682
BtsIMutI CAGTG 6 cut(s) 1682, 2438, 2497, 3382, 3396, 3757
BveI ACCTGC 2 cut(s) 1239, 2755
CaiI CAGNNNCTG 2 cut(s) 2701, 3782
CfoI GCGC 4 cut(s) 2043, 3546, 4105, 4166
Cfr10I RCCGGY 2 cut(s) 50, 3181
Cfr13I GGNCC 5 cut(s) 616, 1336, 1337, 3088, 3184
ClaI ATCGAT 1 cut(s) 3344
CseI GACGC 2 cut(s) 432, 3263
Csp6I GTAC 9 cut(s) 626, 730, 1502, 1915, 2106, 2409, 3248, 3801, 4138
CspAI ACCGGT 1 cut(s) 3181
CviQI GTAC 9 cut(s) 626, 730, 1502, 1915, 2106, 2409, 3248, 3801, 4138
DpnI GATC 8 cut(s) 339, 1764, 2470, 2914, 3439, 3520, 3617, 3981
DpnII GATC 8 cut(s) 337, 1762, 2468, 2912, 3437, 3518, 3615, 3979
DraI TTTAAA 1 cut(s) 1227
DraIII CACNNNGTG 1 cut(s) 2534
DriI GACNNNNNGTC 2 cut(s) 3480, 3640
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 3 cut(s) 116, 1983, 3699
Eam1105I GACNNNNNGTC 2 cut(s) 3480, 3640
EarI CTCTTC 3 cut(s) 116, 1983, 3699
EciI GGCGGA 1 cut(s) 453
Eco130I CCWWGG 6 cut(s) 42, 612, 1521, 3084, 3671, 4045
Eco147I AGGCCT 3 cut(s) 19, 331, 3670
Eco24I GRGCYC 3 cut(s) 1340, 1415, 1804
Eco31I GGTCTC 1 cut(s) 257
Eco32I GATATC 3 cut(s) 1728, 3216, 3748
Eco47I GGWCC 1 cut(s) 3184
Eco47III AGCGCT 1 cut(s) 4165
Eco57I CTGAAG 7 cut(s) 266, 899, 947, 1946, 2663, 2933, 3849
Eco72I CACGTG 1 cut(s) 4082
Eco81I CCTNAGG 1 cut(s) 1596
Eco88I CYCGRG 1 cut(s) 907
EcoNI CCTNNNNNAGG 2 cut(s) 24, 1575
EcoO109I RGGNCCY 1 cut(s) 1337
EcoRI GAATTC 3 cut(s) 277, 712, 2392
EcoRII CCWGG 8 cut(s) 340, 618, 661, 2848, 3103, 3208, 3427, 3636
EcoRV GATATC 3 cut(s) 1728, 3216, 3748
EcoT14I CCWWGG 6 cut(s) 42, 612, 1521, 3084, 3671, 4045
EcoT22I ATGCAT 4 cut(s) 1323, 1398, 1558, 3361
EcoT38I GRGCYC 3 cut(s) 1340, 1415, 1804
ErhI CCWWGG 6 cut(s) 42, 612, 1521, 3084, 3671, 4045
FaqI GGGAC 6 cut(s) 353, 1120, 3175, 3541, 4027, 4156
FauI CCCGC 1 cut(s) 2274
FauNDI CATATG 3 cut(s) 2110, 2199, 2868
FbaI TGATCA 1 cut(s) 3615
FokI GGATG 7 cut(s) 59, 1791, 2848, 3050, 3079, 3527, 4082
FriOI GRGCYC 3 cut(s) 1340, 1415, 1804
FspBI CTAG 7 cut(s) 1476, 1829, 3441, 3531, 3552, 3672, 3771
FspI TGCGCA 1 cut(s) 4104
GlaI GCGC 4 cut(s) 2042, 3545, 4104, 4165
GsaI CCCAGC 4 cut(s) 1066, 2503, 3144, 3171
GsuI CTGGAG 4 cut(s) 34, 492, 2555, 3659
HaeII RGCGCY 3 cut(s) 2044, 3547, 4167
HapII CCGG 7 cut(s) 51, 141, 519, 1356, 2462, 3182, 3932
HgaI GACGC 2 cut(s) 432, 3263
HhaI GCGC 4 cut(s) 2043, 3546, 4105, 4166
Hin1I GRCGYC 1 cut(s) 424
Hin6I GCGC 4 cut(s) 2041, 3544, 4103, 4164
HinP1I GCGC 4 cut(s) 2041, 3544, 4103, 4164
HincII GTYRAC 5 cut(s) 100, 2995, 3279, 3645, 3766
HindII GTYRAC 5 cut(s) 100, 2995, 3279, 3645, 3766
HindIII AAGCTT 2 cut(s) 2329, 3844
HpaII CCGG 7 cut(s) 51, 141, 519, 1356, 2462, 3182, 3932
HphI GGTGA 9 cut(s) 65, 1184, 1877, 3008, 3045, 3349, 3467, 3806, 3870
HpyCH4III ACNGT 6 cut(s) 945, 1407, 2652, 2901, 3739, 3805
HpyCH4IV ACGT 4 cut(s) 116, 1755, 3501, 4081
HpySE526I ACGT 4 cut(s) 116, 1755, 3501, 4081
Hsp92I GRCGYC 1 cut(s) 424
HspAI GCGC 4 cut(s) 2041, 3544, 4103, 4164
Kpn2I TCCGGA 1 cut(s) 140
KpnI GGTACC 1 cut(s) 629
Ksp22I TGATCA 1 cut(s) 3615
Kzo9I GATC 8 cut(s) 337, 1762, 2468, 2912, 3437, 3518, 3615, 3979
LguI GCTCTTC 1 cut(s) 3699
LmnI GCTCC 5 cut(s) 1418, 2038, 2190, 2500, 3176
MaeI CTAG 7 cut(s) 1476, 1829, 3441, 3531, 3552, 3672, 3771
MaeII ACGT 4 cut(s) 116, 1755, 3501, 4081
MalI GATC 8 cut(s) 339, 1764, 2470, 2914, 3439, 3520, 3617, 3981
MbiI CCGCTC 1 cut(s) 4113
MboI GATC 8 cut(s) 337, 1762, 2468, 2912, 3437, 3518, 3615, 3979
MfeI CAATTG 3 cut(s) 1331, 2678, 3198
MflI RGATCY 5 cut(s) 337, 2468, 2912, 3437, 3979
MhlI GDGCHC 8 cut(s) 1340, 1415, 1804, 2497, 2772, 3181, 3818, 4081
MlsI TGGCCA 1 cut(s) 290
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 7 cut(s) 73, 526, 826, 919, 3007, 3502, 3650
MmeI TCCRAC 5 cut(s) 201, 532, 2016, 2500, 3007
Mox20I TGGCCA 1 cut(s) 290
Mph1103I ATGCAT 4 cut(s) 1323, 1398, 1558, 3361
MroI TCCGGA 1 cut(s) 140
MroXI GAANNNNTTC 1 cut(s) 4027
MscI TGGCCA 1 cut(s) 290
MslI CAYNNNNRTG 2 cut(s) 1551, 3267
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 6 cut(s) 70, 742, 838, 1016, 3167, 3779
MspI CCGG 7 cut(s) 51, 141, 519, 1356, 2462, 3182, 3932
MspR9I CCNGG 9 cut(s) 342, 620, 663, 1357, 2850, 3105, 3210, 3429, 3638
MunI CAATTG 3 cut(s) 1331, 2678, 3198
Mva1269I GAATGC 5 cut(s) 942, 970, 1558, 2521, 3142
MvaI CCWGG 8 cut(s) 342, 620, 663, 2850, 3105, 3210, 3429, 3638
NciI CCSGG 1 cut(s) 1357
NcoI CCATGG 3 cut(s) 42, 612, 3084
NdeI CATATG 3 cut(s) 2110, 2199, 2868
NdeII GATC 8 cut(s) 337, 1762, 2468, 2912, 3437, 3518, 3615, 3979
NlaIV GGNNCC 8 cut(s) 339, 627, 1338, 1414, 1735, 3031, 3090, 3205
NmeAIII GCCGAG 1 cut(s) 2430
NmuCI GTSAC 4 cut(s) 1587, 1620, 3377, 3876
NsbI TGCGCA 1 cut(s) 4104
NsiI ATGCAT 4 cut(s) 1323, 1398, 1558, 3361
NspI RCATGY 5 cut(s) 571, 1483, 1567, 1856, 4103
PaeI GCATGC 2 cut(s) 1483, 4103
PaeR7I CTCGAG 1 cut(s) 907
PaqCI CACCTGC 1 cut(s) 2755
PasI CCCWGGG 1 cut(s) 3209
PceI AGGCCT 3 cut(s) 19, 331, 3670
PciI ACATGT 1 cut(s) 1563
PciSI GCTCTTC 1 cut(s) 3699
PctI GAATGC 5 cut(s) 942, 970, 1558, 2521, 3142
PdmI GAANNNNTTC 1 cut(s) 4027
PflFI GACNNNGTC 1 cut(s) 268
PflMI CCANNNNNTGG 1 cut(s) 3434
PinAI ACCGGT 1 cut(s) 3181
PleI GAGTC 7 cut(s) 72, 526, 826, 918, 3007, 3502, 3649
PmaCI CACGTG 1 cut(s) 4082
PmlI CACGTG 1 cut(s) 4082
PpsI GAGTC 7 cut(s) 72, 526, 826, 918, 3007, 3502, 3649
Ppu21I YACGTR 1 cut(s) 4082
PscI ACATGT 1 cut(s) 1563
PshAI GACNNNNGTC 1 cut(s) 1585
PshBI ATTAAT 2 cut(s) 2136, 2562
Psp1406I AACGTT 1 cut(s) 3501
Psp6I CCWGG 8 cut(s) 340, 618, 661, 2848, 3103, 3208, 3427, 3636
PspCI CACGTG 1 cut(s) 4082
PspFI CCCAGC 4 cut(s) 1062, 2499, 3140, 3167
PspGI CCWGG 8 cut(s) 340, 618, 661, 2848, 3103, 3208, 3427, 3636
PspN4I GGNNCC 8 cut(s) 339, 627, 1338, 1414, 1735, 3031, 3090, 3205
PspOMI GGGCCC 1 cut(s) 1336
PspPI GGNCC 5 cut(s) 616, 1336, 1337, 3088, 3184
PstI CTGCAG 6 cut(s) 75, 208, 843, 2553, 2878, 3451
PstNI CAGNNNCTG 2 cut(s) 2701, 3782
PsuI RGATCY 5 cut(s) 337, 2468, 2912, 3437, 3979
PsyI GACNNNGTC 1 cut(s) 268
PvuII CAGCTG 5 cut(s) 70, 742, 838, 3167, 3779
RsaI GTAC 9 cut(s) 627, 731, 1503, 1916, 2107, 2410, 3249, 3802, 4139
RsaNI GTAC 9 cut(s) 626, 730, 1502, 1915, 2106, 2409, 3248, 3801, 4138
RseI CAYNNNNRTG 2 cut(s) 1551, 3267
SapI GCTCTTC 1 cut(s) 3699
Sau3AI GATC 8 cut(s) 337, 1762, 2468, 2912, 3437, 3518, 3615, 3979
Sau96I GGNCC 5 cut(s) 616, 1336, 1337, 3088, 3184
SbfI CCTGCAGG 1 cut(s) 2878
SchI GAGTC 7 cut(s) 73, 526, 826, 919, 3007, 3502, 3650
ScrFI CCNGG 9 cut(s) 342, 620, 663, 1357, 2850, 3105, 3210, 3429, 3638
SdaI CCTGCAGG 1 cut(s) 2878
SduI GDGCHC 8 cut(s) 1340, 1415, 1804, 2497, 2772, 3181, 3818, 4081
SfcI CTRYAG 6 cut(s) 71, 204, 839, 2549, 2874, 3447
Sfr274I CTCGAG 1 cut(s) 907
SinI GGWCC 1 cut(s) 3184
SlaI CTCGAG 1 cut(s) 907
SmiMI CAYNNNNRTG 2 cut(s) 1551, 3267
SmlI CTYRAG 7 cut(s) 24, 164, 907, 1575, 2006, 2235, 4159
SmoI CTYRAG 7 cut(s) 24, 164, 907, 1575, 2006, 2235, 4159
SpeI ACTAGT 1 cut(s) 3551
SphI GCATGC 2 cut(s) 1483, 4103
Sse8387I CCTGCAGG 1 cut(s) 2878
SseBI AGGCCT 3 cut(s) 19, 331, 3670
SsiI CCGC 9 cut(s) 464, 698, 1014, 1100, 2219, 2267, 3335, 3724, 4113
SspI AATATT 3 cut(s) 1056, 2560, 3148
SspMI CTAG 7 cut(s) 1476, 1829, 3441, 3531, 3552, 3672, 3771
StuI AGGCCT 3 cut(s) 19, 331, 3670
StyD4I CCNGG 9 cut(s) 340, 618, 661, 1355, 2848, 3103, 3208, 3427, 3636
StyI CCWWGG 6 cut(s) 42, 612, 1521, 3084, 3671, 4045
TaaI ACNGT 6 cut(s) 945, 1407, 2652, 2901, 3739, 3805
TaiI ACGT 4 cut(s) 119, 1758, 3504, 4084
TaqI TCGA 7 cut(s) 908, 1820, 2345, 3296, 3344, 3732, 4001
TaqII GACCGA 1 cut(s) 2164
TatI WGTACW 3 cut(s) 1501, 1914, 2408
TauI GCSGC 5 cut(s) 700, 1016, 1102, 2221, 3727
TscAI CASTG 6 cut(s) 1689, 2445, 2497, 3382, 3396, 3757
TseFI GTSAC 4 cut(s) 1587, 1620, 3377, 3876
Tsp45I GTSAC 4 cut(s) 1587, 1620, 3377, 3876
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 6 cut(s) 1689, 2445, 2497, 3382, 3396, 3757
Tth111I GACNNNGTC 1 cut(s) 268
Van91I CCANNNNNTGG 1 cut(s) 3434
VneI GTGCAC 2 cut(s) 2768, 4077
VpaK11BI GGWCC 1 cut(s) 3184
VspI ATTAAT 2 cut(s) 2136, 2562
XagI CCTNNNNNAGG 2 cut(s) 24, 1575
XbaI TCTAGA 2 cut(s) 1828, 3440
XceI RCATGY 5 cut(s) 571, 1483, 1567, 1856, 4103
XcmI CCANNNNNNNNNTGG 1 cut(s) 2579
XhoI CTCGAG 1 cut(s) 907
XmaJI CCTAGG 1 cut(s) 3671
XmnI GAANNNNTTC 1 cut(s) 4027
XspI CTAG 7 cut(s) 1476, 1829, 3441, 3531, 3552, 3672, 3771
Zsp2I ATGCAT 4 cut(s) 1323, 1398, 1558, 3361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.