RLG00000036598

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006832
Physical Location & Seq
Forward (+)
82319949 .. 82330031
10083 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036598

Sequence Viewer

Length: 4506 bp
ATGGACACGAATAATTGGAGGCCTCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGAGCCAATTACAGCAGGATTCACGGCAGAGAATTGTCAATAAGATAATGGACACATTGAAGAGGCATCTTCCCTTCTCTGGTCAAGAGGGATTGCATGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATACTGCTGCTACAAGCCAGTCGGATTATCTACGGAAAATTTCTCTGAAAATGCTTACTATGGAGACCAAGTCTCAGAACACAATTGGCAATTCTTTGCAAAGCAACTCTGCTGGTAATAGTAACAGACCCCCTGATCCAGGATCTTTCGGTATGCAAGCCCAAGTTCCCAATCAAGGACAGTCACTCCCTATGCCAGTGCCAGCCAATCAATCTCAACCACGTCAACAACTCTTATCACAGAACATTCAGAATAACATTCCGCCCAATTCTGCTGGCTTATCATCTGCATTATCTCCTGCATCTGGGCTCACCCAGACTCCTGTACCGAGTATTGTTGGAAATGCAAACTTGCAAAGTATGTCGGGGACATCACAGAATTCATTGGGGAACTCCATGGGCCAGGGGGTAACTTCAAATATGTTTGCTAATTCCCAAAGGCAAATGCCAGGACGACAACAGGTAGTTCCGCAGCAGCAACAGCAGCAACCCCAGAATTCACAGCAGTATATGTACCAGCAACAGTTACAACAACAGCTCATGAAGCCAAAATTTCAGCAGCCGGGAAACATCCAACTTGTGCAAAATCATATGCAACAGCAACAACAGCAGCAGCAGAACTTATTACAATCACCTCAGTTGCAATCTTCTCAGCAATCTGTTATGCAACCTCCTGCAGTGCAGTCGTCTCTCTCTAGTCTTCAGCAGAATCAACAATCTGCTATGCAACACTCTTCACAAGCCATGCTTCAGCAGCATCCTCAATCTGTTCTGCGGCAGCAACAGCAGCCTCAACAGTCTTCTGTTGTCCATCAGCAGCAAACATCAATCCCACAGCAGCCAATATTGGCCCCACAGCAGCAGCAGCAACAGCCACAGCTTATGGTGCAACAGCCAAATTCAACAAACTTGCCGCAGAATCAGCTTATTGGACAACAAAACAATGTAGGGGACATGCAGCAACAGCAACAGCAAAGGTTACTAAGCCAGCAGAATAATATGTTAAACTTGCAACAGCAGCAGCAGCAGCAGCAGCAGCAACAACAACACCAATTAATGGCTCAGCAAAACAACCTCTCAAATATGCATCAGCAACAGTTGGGCCCTCAGAGTAATGTTACTGGATTACAGCAACAGCAGCACCTTGGTACTCAAACTGGTAACTCAAGCATGCAAACTAATCAGCACTCTGCACACATGCTGCAATCCAAGGTTCAGCAACAACATCAGAGTCCAGCATTACCGATTCAAGGACATCAGTCCCAACCTCAGGCGTCACAGCAACAATTATTATCACAGATTCAATCTCAGCCTGCACAGATACAACAACAGATGGGTATGCAACAGCAATCAAACCCGCTACAACGAGATATGCAGCAAAGGATCCAAGCATCAGGTCAGGTATCAGGATCCATGCTTCAACCACCAAATGTAATGGATCAGCAAAAGCAGTTATATCAAGCTCAAAGAGCCCTTCCTGAGACGTCATCAACGTCTCTTGATTCCACAGCCCAGACTGGACATACTAATGGTGCTGATTGGCAAGAGGAGGTCTATCAAAAGATCAAAGTCATGAAGGATATGTACTACCCCGAACTAAGTGAAATGTACCAAAAAATTGCTTCTAAACTTCATCAGCATGACTCCCTTCCACAACAGCCAAAGTCAGATCAGCTTGAGAAGCTGAAGATGTTTAAGACCATGCTGGAGCGCCTGATAACAGTCTTACAAATTTCCAAAACTAACATAACACCTAGTTTGAAGGAGAAGTTGGGTTTATACGAGAAGCAGATAATAAATTTTATTAATACAAATAGACCCAGGAAGCCTGTTTCTTCTCTGCCTGTTTCTTCTCTGCAGCCAGGGCAGCTCCCCCCACCGCATATGCAGTCCATGCAGCAGTCACAATCCCAGATTACTCAAGTGCAATCTCATGAAAATCAGATGAACCCACAATTGCAATCCCTGAACTTGCAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACGGGCTTGCAGCAAAATTCTATGTCTTCTTTATCTGGGGTTTCAACAGCACAGCAAAACATGATGAATCCGTTGCAGCCAAGTTCCAATATGGATCCAGGACAAGGAACTGCACTGAACTCACTGCAGCAGGTCCCTGTGGGCTCTATCCAACAGACTCCTGTCAGTGCTCCCCAACAAGCTAACATGAATGCCTTATCATCTCAGAATGGGATTAACATGCTGCAGAGCAACATTAATCCCCTTCAGTCAAATTCTGGTATGCTTCATAGCCAACATCTGAAACAGCAGGAACAGCAATTGTATCAGAATCAACTCAAGCAACAATATCAACAACGACAGATGCAGCAGCAGCAGATGATGCAGAAGCAGCATATAATTCAACAACAACAGCAGCAACAGCAACAACAGCTACAACAGCAAGCAAAGCAGCAGCTGCCTGGACAGTTGCAGGCACACCAAATGCCACAGCTTCACCAAATCAGTGATGTTAATGATGTGAAAGTAAGGCCGGGGATGGGTGTTAAGCCAGGGGCCTTTCAGCAACAAATGTCTGTAGGCCAGCGTGCTTATCCTCATTCGCAATTAAAACCAGGATCTCCATATCCTATTACATCAAGCAACCAACTCCTTCAGGCTCAATCTCCTCAACTGTCACAGCATTCTTCTCCACAAGTTGAGCAGCCGTTGTTAGTCCCAAAAGCTCAAAATGCTAGTTCACCATTTGTCATCCCATCTCCTTCAACTCCCATAGCTCCATCACCTATGCCAGGAGATTCAGAGAAACCTTCCTCACTCGCAAATGCTGGGAATGTTGGACATCAACAAACAACTGTTGTCGGAGCTCAGGTCCAGTCCCTTGCCATCGGCACCCCTGGGATATCAGCCTCTCCTTTGCTTGCTGAATTTATCGGTCCAGATGGCACTCATGCTAATGCTTTGTCAGCTATATCTGGCAAGTCAAGTGTTACAGAGCAACCTCTTGAACGCTTGATTAAAGCGGTCAAGTCAATATCACCAAAGGCATTAAGTGCATCTGTCAGTGACATAGGCTCAGTAGTGAGTATGATTGATAGGATAGCAGGTTCAGCACCAGGTAATGGATCTAGAGCTGCAGTTGGTGAGGATCTGGTTGCTATGACAAAATGTCGTCTACAGGCGAGAAACTTCATCACTCATGATGGAACAACAAATGGGACAAGGAAAATGAGGCGATATACTAGTGCCATGCCCTTAAATGTTGTGTCATCAGGTGGCAGCATGAATGATAGTTTCAAGCAGTTGACCTGTTCAGAGACATCTGACCTGGAGTCAACTGCAACGTCTCGTATCAAAAGGCCTAGGATTGAGGCTAATCATGCTCTTATGGAAGAAATAAGAGAAATAAACCAGCGGCTTATAGATACCGTTGTTGATATCAGTGATGAAGATGTTGATCCAAGTGCTGCTGCTGCTGAAGGGGGTGAAGGAACCATCGTCAAGTGCTCTTTTGATGCTGTGGCTCTCAGTCCCAACTTGAAATCGCAGTATGCTTCAGCACAAATGTCTCCGATTCAGCCATTAAGACTGCTTGTTCCGACAAATTATCCCAACTGCTCTCCAATACTGTTAGACAAGTTTCCGGTTGAAGTCAGTAAGGAGTATGAAGATCTTTCTGTGAAGGCAAAATCAAGGTTTAGCATCTCTTTACGAAGCATTTCGCAGCCCATGTCCCTTGGGGAGATAGCAAGGGCTTGGGATAATTGTGCACGTACAGTTATTTCTGAGCATGCACAGCGAAGTGGTGGAGGCAGCTTCAGCTCAAAGTTATACCTGGGTGGTATAGTTGATACTGTAAATAATGTCAAAGTGTCTTTCAGAATGAAGATGCGGTATTCTGCTGTCTGGTTTGCGCGCCGAAGCCTTATTCTCTCAAGTCAGGACAGAAGCCGCTCTCCTTCTCAGACGAAACTTGTGCAGAAGGTTGTGAGGCTTGTCGAGGATCAATATGGATGGAATGACGAACAAACTGAGAATCGAACTTTTGTGGAGATTTATTCCATAGCTGCTGGCTCATGGAGTAGTCCTAGTGAGTTATTCCGGGAGATAATGATGCCAAAGAGTTTGGATTGGGATTCATTTAAGTCATGGCATGGAGACTTGTCATTGCGATTGTCTGTTTCAGGAGATGGGAAATCCCTTGCTCTGTTCATGAGATTCGTAGTGCTAGTACATAACTACAATCTTTTGCTTTTAAGTGCTACTGTATCTTCTTTGGATGCAAGTTCTAATCTAAGTGCCTTTGTGTTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1502

Amino Acids

166.1

Weight (kDa)

9.44

Isoelectric Point (pI)

70.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 98 1.5e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1688
Acc36I ACCTGC 2 cut(s) 2332, 3284
AccB1I GGYRCC 1 cut(s) 3080
AccB7I CCANNNNNTGG 2 cut(s) 1258, 3311
AccBSI CCGCTC 1 cut(s) 4143
AccI GTMKAC 1 cut(s) 3364
AccII CGCG 1 cut(s) 4105
AcuI CTGAAG 8 cut(s) 887, 935, 1907, 2441, 2828, 3687, 3729, 3991
AcyI GRCGYC 2 cut(s) 1475, 1685
AfaI GTAC 7 cut(s) 528, 716, 1351, 1787, 1811, 3964, 4422
AhdI GACNNNNNGTC 2 cut(s) 3357, 3520
AhlI ACTAGT 1 cut(s) 3431
AjiI CACGTC 1 cut(s) 425
AjuI GAANNNNNNNTTGG 2 cut(s) 1955, 1987
AloI GAACNNNNNNTCC 2 cut(s) 3280, 3312
Alw21I GWGCWC 4 cut(s) 2383, 3058, 3698, 3961
Alw26I GTCTC 9 cut(s) 260, 279, 894, 1676, 1701, 3500, 3540, 3762, 4341
Alw44I GTGCAC 1 cut(s) 3957
AlwNI CAGNNNCTG 1 cut(s) 2647
AoxI GGCC 8 cut(s) 20, 601, 1050, 1303, 2720, 2745, 2770, 3548
ApaI GGGCCC 1 cut(s) 1307
ApaLI GTGCAC 1 cut(s) 3957
AseI ATTAAT 3 cut(s) 1256, 2007, 2448
Asp700I GAANNNNTTC 1 cut(s) 3907
AspA2I CCTAGG 1 cut(s) 3551
AspLEI GCGC 3 cut(s) 1914, 4105, 4107
AspS9I GGNCC 8 cut(s) 601, 1051, 1303, 1304, 2344, 2745, 3061, 3125
AsuC2I CCSGG 3 cut(s) 765, 2724, 4292
AsuHPI GGTGA 8 cut(s) 505, 825, 2678, 2922, 2964, 3219, 3344, 3686
AvaII GGWCC 3 cut(s) 2344, 3061, 3125
AvrII CCTAGG 1 cut(s) 3551
AxyI CCTNAGG 1 cut(s) 1470
BaeGI GKGCMC 2 cut(s) 1307, 3961
BaeI ACNNNNGTAYC 4 cut(s) 510, 543, 3606, 3639
BamHI GGATCC 3 cut(s) 1584, 1610, 2305
BanI GGYRCC 1 cut(s) 3080
BanII GRGCYC 5 cut(s) 513, 1307, 1675, 2357, 3058
BarI GAAGNNNNNNTAC 2 cut(s) 1769, 1801
BbsI GAAGAC 3 cut(s) 893, 993, 2229
Bbv12I GWGCWC 4 cut(s) 2383, 3058, 3698, 3961
BceAI ACGGC 2 cut(s) 104, 2881
BcgI CGANNNNNNTGC 4 cut(s) 867, 901, 4147, 4181
BcnI CCSGG 3 cut(s) 765, 2724, 4292
BcoDI GTCTC 9 cut(s) 260, 279, 894, 1676, 1701, 3500, 3540, 3762, 4341
BcuI ACTAGT 1 cut(s) 3431
BfaI CTAG 8 cut(s) 897, 1956, 2925, 3318, 3432, 3552, 4278, 4418
BfmI CTRYAG 7 cut(s) 876, 2057, 2336, 2435, 2766, 3324, 3365
BfoI RGCGCY 1 cut(s) 1915
BfuAI ACCTGC 2 cut(s) 2332, 3284
BglII AGATCT 1 cut(s) 3859
BlnI CCTAGG 1 cut(s) 3551
BlpI GCTNAGC 1 cut(s) 1263
Bme18I GGWCC 3 cut(s) 2344, 3061, 3125
BmeRI GACNNNNNGTC 2 cut(s) 3357, 3520
BmgBI CACGTC 1 cut(s) 425
BmgT120I GGNCC 8 cut(s) 601, 1051, 1303, 1304, 2344, 2745, 3061, 3125
BoxI GACNNNNGTC 2 cut(s) 1459, 2372
BpiI GAAGAC 3 cut(s) 893, 993, 2229
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 2 cut(s) 1928, 3539
Bpu10I CCTNAGC 1 cut(s) 3057
Bpu1102I GCTNAGC 1 cut(s) 1263
BpuEI CTTGAG 7 cut(s) 12, 152, 1351, 1898, 2106, 2513, 4108
BpuMI CCSGG 3 cut(s) 765, 2724, 4292
BsaAI YACGTR 1 cut(s) 3962
BsaBI GATNNNNATC 1 cut(s) 3645
BsaHI GRCGYC 2 cut(s) 1475, 1685
BsaI GGTCTC 1 cut(s) 260
BsaWI WCCGGW 1 cut(s) 3832
BsaXI ACNNNNNCTCC 8 cut(s) 257, 287, 372, 402, 505, 535, 3045, 3075
Bse1I ACTGG 6 cut(s) 220, 398, 1327, 1363, 1723, 3064
Bse21I CCTNAGG 1 cut(s) 1470
Bse3DI GCAATG 2 cut(s) 51, 4355
Bse8I GATNNNNATC 1 cut(s) 3645
BseGI GGATG 7 cut(s) 55, 771, 958, 2733, 2940, 4208, 4474
BseJI GATNNNNATC 1 cut(s) 3645
BseMI GCAATG 2 cut(s) 51, 4355
BseNI ACTGG 6 cut(s) 220, 398, 1327, 1363, 1723, 3064
BsePI GCGCGC 1 cut(s) 4103
BseRI GAGGAG 4 cut(s) 15, 79, 1763, 2847
BseSI GKGCMC 2 cut(s) 1307, 3961
BseYI CCCAGC 1 cut(s) 3017
BsgI GTGCAG 5 cut(s) 902, 1377, 1500, 2307, 4187
Bsh1236I CGCG 1 cut(s) 4105
BshFI GGCC 8 cut(s) 22, 603, 1052, 1305, 2722, 2747, 2772, 3550
BshNI GGYRCC 1 cut(s) 3080
BsiHKAI GWGCWC 4 cut(s) 2383, 3058, 3698, 3961
BsiSI CCGG 4 cut(s) 764, 2723, 3833, 4291
BslFI GGGAC 9 cut(s) 583, 1166, 1447, 2330, 2891, 3052, 3421, 3705, 3907
BsmAI GTCTC 9 cut(s) 260, 279, 894, 1676, 1701, 3500, 3540, 3762, 4341
BsmBI CGTCTC 4 cut(s) 894, 1676, 1701, 3540
BsmFI GGGAC 9 cut(s) 583, 1166, 1447, 2330, 2891, 3052, 3421, 3705, 3907
BsmI GAATGC 2 cut(s) 2407, 2872
BsnI GGCC 8 cut(s) 22, 603, 1052, 1305, 2722, 2747, 2772, 3550
Bso31I GGTCTC 1 cut(s) 260
Bsp120I GGGCCC 1 cut(s) 1303
Bsp1286I GDGCHC 8 cut(s) 513, 1307, 1675, 2357, 2383, 3058, 3698, 3961
Bsp1720I GCTNAGC 1 cut(s) 1263
Bsp19I CCATGG 1 cut(s) 597
BspANI GGCC 8 cut(s) 22, 603, 1052, 1305, 2722, 2747, 2772, 3550
BspFNI CGCG 1 cut(s) 4105
BspHI TCATGA 5 cut(s) 741, 1773, 2134, 3388, 4401
BspMAI CTGCAG 5 cut(s) 880, 2061, 2340, 2439, 3328
BspMI ACCTGC 2 cut(s) 2332, 3284
BspT107I GGYRCC 1 cut(s) 3080
BspTNI GGTCTC 1 cut(s) 260
BsrBI CCGCTC 1 cut(s) 4143
BsrDI GCAATG 2 cut(s) 51, 4355
BsrI ACTGG 6 cut(s) 220, 398, 1327, 1363, 1723, 3064
BssHII GCGCGC 1 cut(s) 4103
BssNI GRCGYC 2 cut(s) 1475, 1685
BssT1I CCWWGG 5 cut(s) 597, 1345, 1410, 3551, 3925
Bst6I CTCTTC 2 cut(s) 119, 940
BstACI GRCGYC 2 cut(s) 1475, 1685
BstAPI GCANNNNNTGC 5 cut(s) 50, 2095, 2572, 2647, 3242
BstBAI YACGTR 1 cut(s) 3962
BstDSI CCRYGG 1 cut(s) 597
BstENI CCTNNNNNAGG 3 cut(s) 27, 339, 2979
BstF5I GGATG 7 cut(s) 55, 771, 958, 2733, 2940, 4208, 4474
BstFNI CGCG 1 cut(s) 4105
BstH2I RGCGCY 1 cut(s) 1915
BstHHI GCGC 3 cut(s) 1914, 4105, 4107
BstMAI GTCTC 9 cut(s) 260, 279, 894, 1676, 1701, 3500, 3540, 3762, 4341
BstNSI RCATGY 5 cut(s) 1159, 1375, 1402, 2434, 3983
BstPAI GACNNNNGTC 2 cut(s) 1459, 2372
BstSFI CTRYAG 7 cut(s) 876, 2057, 2336, 2435, 2766, 3324, 3365
BstSLI GKGCMC 2 cut(s) 1307, 3961
BstUI CGCG 1 cut(s) 4105
BstV2I GAAGAC 3 cut(s) 893, 993, 2229
BstX2I RGATCY 8 cut(s) 344, 1584, 1610, 2305, 2807, 3314, 3337, 3859
BstYI RGATCY 8 cut(s) 344, 1584, 1610, 2305, 2807, 3314, 3337, 3859
Bsu36I CCTNAGG 1 cut(s) 1470
BsuRI GGCC 8 cut(s) 22, 603, 1052, 1305, 2722, 2747, 2772, 3550
BtgI CCRYGG 1 cut(s) 597
BtrI CACGTC 1 cut(s) 425
BtsCI GGATG 7 cut(s) 55, 771, 958, 2733, 2940, 4208, 4474
BtsI GCAGTG 2 cut(s) 885, 2333
BtsIMutI CAGTG 8 cut(s) 405, 885, 2324, 2333, 2383, 2701, 3259, 3637
BveI ACCTGC 2 cut(s) 2332, 3284
CaiI CAGNNNCTG 1 cut(s) 2647
CciI TCATGA 5 cut(s) 741, 1773, 2134, 3388, 4401
CfoI GCGC 3 cut(s) 1914, 4105, 4107
Cfr13I GGNCC 8 cut(s) 601, 1051, 1303, 1304, 2344, 2745, 3061, 3125
CseI GACGC 1 cut(s) 1464
CsiI ACCWGGT 1 cut(s) 3304
Csp6I GTAC 7 cut(s) 527, 715, 1350, 1786, 1810, 3963, 4421
CviQI GTAC 7 cut(s) 527, 715, 1350, 1786, 1810, 3963, 4421
DriI GACNNNNNGTC 2 cut(s) 3357, 3520
Eam1104I CTCTTC 2 cut(s) 119, 940
Eam1105I GACNNNNNGTC 2 cut(s) 3357, 3520
EarI CTCTTC 2 cut(s) 119, 940
EciI GGCGGA 1 cut(s) 453
Ecl136II GAGCTC 1 cut(s) 3056
Eco130I CCWWGG 5 cut(s) 597, 1345, 1410, 3551, 3925
Eco147I AGGCCT 2 cut(s) 22, 3550
Eco24I GRGCYC 5 cut(s) 513, 1307, 1675, 2357, 3058
Eco31I GGTCTC 1 cut(s) 260
Eco32I GATATC 2 cut(s) 3093, 3628
Eco47I GGWCC 3 cut(s) 2344, 3061, 3125
Eco53kI GAGCTC 1 cut(s) 3056
Eco57I CTGAAG 8 cut(s) 887, 935, 1907, 2441, 2828, 3687, 3729, 3991
Eco81I CCTNAGG 1 cut(s) 1470
EcoICRI GAGCTC 1 cut(s) 3056
EcoNI CCTNNNNNAGG 3 cut(s) 27, 339, 2979
EcoO109I RGGNCCY 3 cut(s) 1304, 2344, 2745
EcoRI GAATTC 2 cut(s) 580, 697
EcoRV GATATC 2 cut(s) 3093, 3628
EcoT14I CCWWGG 5 cut(s) 597, 1345, 1410, 3551, 3925
EcoT22I ATGCAT 1 cut(s) 1290
EcoT38I GRGCYC 5 cut(s) 513, 1307, 1675, 2357, 3058
ErhI CCWWGG 5 cut(s) 597, 1345, 1410, 3551, 3925
Esp3I CGTCTC 4 cut(s) 894, 1676, 1701, 3540
FalI AAGNNNNNCTT 2 cut(s) 933, 965
FaqI GGGAC 9 cut(s) 583, 1166, 1447, 2330, 2891, 3052, 3421, 3705, 3907
FauI CCCGC 1 cut(s) 1566
FauNDI CATATG 2 cut(s) 792, 2085
FblI GTMKAC 1 cut(s) 3364
FokI GGATG 7 cut(s) 62, 758, 945, 2740, 2927, 4215, 4481
FriOI GRGCYC 5 cut(s) 513, 1307, 1675, 2357, 3058
FspBI CTAG 8 cut(s) 897, 1956, 2925, 3318, 3432, 3552, 4278, 4418
GlaI GCGC 3 cut(s) 1913, 4104, 4106
GsaI CCCAGC 1 cut(s) 3021
GsuI CTGGAG 2 cut(s) 1928, 3539
HaeII RGCGCY 1 cut(s) 1915
HaeIII GGCC 8 cut(s) 22, 603, 1052, 1305, 2722, 2747, 2772, 3550
HapII CCGG 4 cut(s) 764, 2723, 3833, 4291
HgaI GACGC 1 cut(s) 1464
HhaI GCGC 3 cut(s) 1914, 4105, 4107
Hin1I GRCGYC 2 cut(s) 1475, 1685
Hin6I GCGC 3 cut(s) 1912, 4103, 4105
HinP1I GCGC 3 cut(s) 1912, 4103, 4105
HincII GTYRAC 3 cut(s) 428, 3495, 3525
HindII GTYRAC 3 cut(s) 428, 3495, 3525
HpaII CCGG 4 cut(s) 764, 2723, 3833, 4291
HphI GGTGA 8 cut(s) 505, 825, 2678, 2922, 2964, 3219, 3344, 3686
Hpy166II GTNNAC 6 cut(s) 428, 2930, 3365, 3495, 3525, 3959
Hpy8I GTNNAC 6 cut(s) 428, 2930, 3365, 3495, 3525, 3959
HpyCH4IV ACGT 5 cut(s) 424, 1685, 1694, 3533, 3961
HpySE526I ACGT 5 cut(s) 424, 1685, 1694, 3533, 3961
Hsp92I GRCGYC 2 cut(s) 1475, 1685
HspAI GCGC 3 cut(s) 1912, 4103, 4105
LmnI GCTCC 6 cut(s) 66, 1909, 2076, 2386, 2971, 3053
MabI ACCWGGT 1 cut(s) 3304
MaeI CTAG 8 cut(s) 897, 1956, 2925, 3318, 3432, 3552, 4278, 4418
MaeII ACGT 5 cut(s) 424, 1685, 1694, 3533, 3961
MbiI CCGCTC 1 cut(s) 4143
MfeI CAATTG 3 cut(s) 285, 2156, 2510
MflI RGATCY 8 cut(s) 344, 1584, 1610, 2305, 2807, 3314, 3337, 3859
MhlI GDGCHC 8 cut(s) 513, 1307, 1675, 2357, 2383, 3058, 3698, 3961
MlyI GAGTC 5 cut(s) 514, 1441, 1838, 2362, 3530
MmeI TCCRAC 7 cut(s) 204, 520, 799, 2386, 3007, 3031, 3812
Mph1103I ATGCAT 1 cut(s) 1290
MroXI GAANNNNTTC 1 cut(s) 3907
MslI CAYNNNNRTG 3 cut(s) 1728, 1839, 3144
MspA1I CMGCKG 2 cut(s) 2647, 3604
MspI CCGG 4 cut(s) 764, 2723, 3833, 4291
MunI CAATTG 3 cut(s) 285, 2156, 2510
Mva1269I GAATGC 2 cut(s) 2407, 2872
MvnI CGCG 1 cut(s) 4105
NciI CCSGG 3 cut(s) 765, 2724, 4292
NcoI CCATGG 1 cut(s) 597
NdeI CATATG 2 cut(s) 792, 2085
NmuCI GTSAC 5 cut(s) 384, 1476, 2103, 2865, 3254
NsiI ATGCAT 1 cut(s) 1290
NspI RCATGY 5 cut(s) 1159, 1375, 1402, 2434, 3983
PaeI GCATGC 2 cut(s) 1375, 3983
PagI TCATGA 5 cut(s) 741, 1773, 2134, 3388, 4401
PasI CCCWGGG 1 cut(s) 3086
PauI GCGCGC 1 cut(s) 4103
PceI AGGCCT 2 cut(s) 22, 3550
PcsI WCGNNNNNNNCGW 1 cut(s) 1691
PctI GAATGC 2 cut(s) 2407, 2872
PdmI GAANNNNTTC 1 cut(s) 3907
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 2 cut(s) 1258, 3311
PfoI TCCNGGA 3 cut(s) 340, 2308, 4290
PleI GAGTC 5 cut(s) 514, 1440, 1838, 2362, 3529
PpsI GAGTC 5 cut(s) 514, 1440, 1838, 2362, 3529
Ppu21I YACGTR 1 cut(s) 3962
PpuMI RGGWCCY 1 cut(s) 2344
PshAI GACNNNNGTC 2 cut(s) 1459, 2372
PshBI ATTAAT 3 cut(s) 1256, 2007, 2448
Psp124BI GAGCTC 1 cut(s) 3058
Psp5II RGGWCCY 1 cut(s) 2344
PspFI CCCAGC 1 cut(s) 3017
PspOMI GGGCCC 1 cut(s) 1303
PspPI GGNCC 8 cut(s) 601, 1051, 1303, 1304, 2344, 2745, 3061, 3125
PspPPI RGGWCCY 1 cut(s) 2344
PstI CTGCAG 5 cut(s) 880, 2061, 2340, 2439, 3328
PstNI CAGNNNCTG 1 cut(s) 2647
PsuI RGATCY 8 cut(s) 344, 1584, 1610, 2305, 2807, 3314, 3337, 3859
PsyI GACNNNGTC 1 cut(s) 271
PteI GCGCGC 1 cut(s) 4103
PvuII CAGCTG 1 cut(s) 2647
RsaI GTAC 7 cut(s) 528, 716, 1351, 1787, 1811, 3964, 4422
RsaNI GTAC 7 cut(s) 527, 715, 1350, 1786, 1810, 3963, 4421
RseI CAYNNNNRTG 3 cut(s) 1728, 1839, 3144
SacI GAGCTC 1 cut(s) 3058
Sau96I GGNCC 8 cut(s) 601, 1051, 1303, 1304, 2344, 2745, 3061, 3125
SchI GAGTC 5 cut(s) 514, 1441, 1838, 2362, 3530
SduI GDGCHC 8 cut(s) 513, 1307, 1675, 2357, 2383, 3058, 3698, 3961
SexAI ACCWGGT 1 cut(s) 3304
SfcI CTRYAG 7 cut(s) 876, 2057, 2336, 2435, 2766, 3324, 3365
SinI GGWCC 3 cut(s) 2344, 3061, 3125
SmiMI CAYNNNNRTG 3 cut(s) 1728, 1839, 3144
SmlI CTYRAG 7 cut(s) 27, 167, 1366, 1877, 2121, 2528, 4123
SmoI CTYRAG 7 cut(s) 27, 167, 1366, 1877, 2121, 2528, 4123
SpeI ACTAGT 1 cut(s) 3431
SphI GCATGC 2 cut(s) 1375, 3983
SseBI AGGCCT 2 cut(s) 22, 3550
SspI AATATT 1 cut(s) 1047
SspMI CTAG 8 cut(s) 897, 1956, 2925, 3318, 3432, 3552, 4278, 4418
SstI GAGCTC 1 cut(s) 3058
StuI AGGCCT 2 cut(s) 22, 3550
StyI CCWWGG 5 cut(s) 597, 1345, 1410, 3551, 3925
TaiI ACGT 5 cut(s) 427, 1688, 1697, 3536, 3964
TaqI TCGA 2 cut(s) 4188, 4228
TaqII GACCGA 1 cut(s) 3113
TatI WGTACW 2 cut(s) 1785, 4420
TauI GCSGC 4 cut(s) 979, 1117, 3607, 4143
TscAI CASTG 8 cut(s) 405, 885, 2331, 2340, 2383, 2701, 3259, 3637
TseFI GTSAC 5 cut(s) 384, 1476, 2103, 2865, 3254
Tsp45I GTSAC 5 cut(s) 384, 1476, 2103, 2865, 3254
TspGWI ACGGA 2 cut(s) 250, 2271
TspRI CASTG 8 cut(s) 405, 885, 2331, 2340, 2383, 2701, 3259, 3637
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 2 cut(s) 1258, 3311
VneI GTGCAC 1 cut(s) 3957
VpaK11BI GGWCC 3 cut(s) 2344, 3061, 3125
VspI ATTAAT 3 cut(s) 1256, 2007, 2448
XagI CCTNNNNNAGG 3 cut(s) 27, 339, 2979
XbaI TCTAGA 1 cut(s) 3317
XceI RCATGY 5 cut(s) 1159, 1375, 1402, 2434, 3983
XmaJI CCTAGG 1 cut(s) 3551
XmiI GTMKAC 1 cut(s) 3364
XmnI GAANNNNTTC 1 cut(s) 3907
XspI CTAG 8 cut(s) 897, 1956, 2925, 3318, 3432, 3552, 4278, 4418
ZraI GACGTC 1 cut(s) 1686
Zsp2I ATGCAT 1 cut(s) 1290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.