Rroxscaffold_1G00005810

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0006832
Physical Location & Seq
Reverse (-)
7843122 .. 7851026
7905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005810.1

Sequence Viewer

Length: 4050 bp
ATGGACACGAATAATTGGAGGCCCCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGAGCCAATTACAGCAGGATTCGCGGCAGAGAATCGTCAATAAGATAATGGATACATTGAAGAGGCATCTTCCCTTCTCCGGTCAAGAGGGATTGCATGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATACTGCTGCTACAAGCCAGTCGGATTATCTACGGAAAATTTCTCTGAAAATGCTCACTATGGAGACCAAGTCTCAGAACACAATTGGCACTTCTTTACAAAACAACTCTGCTGGTAATAGTAACAGGCCCCCTGATCCAGGATCTTTCATGCAAGCCCAAGTTCCCAATCAAGGACAGTCACTCCCTATGCCAGTGCCAGCCAATCAATCTCAACCACGCCAACAACTCTTATCACAGAACATTCAGAATAACATTCCGCCCAATTCTACTGGCTTATCATCTGCATTATCTCCTGCATCTGGGCTCACCCAGACTCCTGTACCGAGTATTGTTGGAAATGCAAACTTGCAAAGTATGTCAGGGACATCACAGAATTCATTGGGGAACTCCATAGGCCAGGGGGTAACTTCAAATATGTTTGCTAATTCCCAAAGGCAAATGCCAGGACGACAACAGGTAGTTCAGCAGCAGCAACAGCAGCAGCCCCAGAATTCACAGCAGTATATCTACCAGCAACAGTTACAACAACAGCTCATGAAGCCAAAATTTCCGCAGCAGGGAAACATCCAACTTGTGCAAAATCATATGCAACAGCAACAACAACAGCAGCAGAACTTATTACAACCACCTCAGTTGCAATCTTCTCAGCAATCTGTTATGCAACCTCCTGCAGTGCAGTCGTCTCTCTCTAGTCTTCAGCAGAATCAACAATCTGCTATGCAACACTCTTCACAAGCCATGCTTCAGCAGCATCCTCAATCTGTTCTGCGGCAGCAACAGCAGCCTCAACAGTCTTCTGTTGTCCATCAGCAGCAAACACCAATCCCACAGCAGCCAATATTGCCCCCACAGCAGCAGCAGCAACAGCCACAGCTTATGGTGCAACAGCCAAATTCAACAAACTTGCAACAGAATCAGCTTATTGGACAACAAAACAATGTAGGGGACATGCAGCAACAGCAAAGGTTACTAAGCCAGCAGAATAATATGTTAAACTTGCAACAGCAGCAGCAGCAGCAGCAACAACAACACCAATTAATGGCTCAGCAAAACAACCTCTCAAATATGCATCAGCAACAGTTGGGCCCTCAGAGTAATGTTACTGGATTACAGCAACAGCAGCACCTTGGTACTCAAACTGGTAACTCAAGCATGCAAACTAATCAGCACTCTGCACACATGCTGCAATCCAAGGTTCAGCAACAACATCAGAGTCCAGCATTACCGATTCAAGGACATCAGTCCCAACCTCAGGCGTCACAGCAACAATTATTATCACAGATTCAATCTCAGCCTGCACAGATACAACAACAGATGGGTATGCAACAGCAATCAAACCCGCTACAACGAGATATGCAGCAAAGGATCCAAGCATCAGGTCAGGTATCAGGATCCATGCTTCAACCACCAAATGTAATGGATCAGCAAAAGCAGTTATATCAAGCTCAAAGAGCCCTTCCTGAGACGTCATCAACGTCTCTAGATTCCACAGCCCAGACTGGACATACTAATGGTGCTGATTGGCAAGAGGAGGTCTATCAAAAGATCAAAGTCATGAAGGATATGTACTACCCCGAACTAAGTGAAATGTACCAAAAAATTGCTACTAAACTTCATCAGCACGACTCCCTTCCACAACAGCCAAAGTCAGATCAGCTTGAGAAGCTGAAGATGTTTAAGACCATGCTGGAGCGCCTGATAACAGTCTTACAAGTTTCCAAAGCTAGCATATCACCTAGTTTGAAGGAGAAGTTGGGTTTATACGAGAAGCAGATAATAAATTTTATTAATACAAATAGACCCAGGAAGCCTGTTTCTTCTCTGCCGCCAGGGCAGCTCCCCCCACCGCATATGCAGTCCATGCAGCAGTCACAATCCCAGATTACGCAAGTGCAAGCTCATGAAAATCAGATGAACCCACAATTGCAATCCCTGAACTTGCAAGGTTCTGTGCCAACAATGCCGCAGAACAATATGACGGGCTTGCAGCAAAATTCTATGTCTTCTTTATCCGCGGTTTCAACAGCACAGCAAAACATGATGAATCCGTTGCAGCCAAGTTCCAATATGGATCCAGGACAAGGAACCGCACTGAACTCACTGCAGCAGGTCCCTGTGGGCTCTATCCAACAGACTCCTGTCAGTGCTCCCCAACAAGCTAACATGAATGCCTTATCATCACAGAATGGGATTAACATGCTGCAGAGCAACATTAATCCCCTTCAGTCAAATTCTGGTATGCTTCATAGCCAACATCTGAAACAGCAGGAACAACAGCAATTGTATCAGAATCAACTCAAGCAACAACAATATCAGCAACGACACATGCAGCAGCAGCAGCAGCAGCAGTTGATGCAGAAGCAGCATATAATTCAACAACAACAGCAGCAACAGCAACAGCAACAGCAACAACAGCTACAACAGCAAGCAAAGCAGCAGCTGCCTGGACAGTTGCAGGCACACCAAATGCCACAGCTTCACCAAATCAGTGATGTTAACGATGTGAAAGTAAGGCCGGGGATGGTTGTTAAGCCAGGGGCCTTTCAGCAACAAATGTCTGTAGGCCAGCGTGCTTATCCTCATTCGCAATTAAAACCAGGATCTCCATATCCCATTACATCAAGCAACCAACTCCTTCAGGCTCAATCTCCTCAACTGTCACAGCATTCTTCTCCACAAGTTGACCAGCAGTTGTTAGTCCCAAAAGCTCAAAATGCTAGTTCACCATTTGTCATCCCATCTCCTTCAACTCCCATAGCTCCATCACCTATGCCAGGAGATTCAGAGAAACCTTCCTCACTCGCAAATGCTGGGAATGTTGGACATCAACAAACAACTGTTGTCGGAGCTCAGGTCCAGTCCCTTGCCATTGGCACCCCTGGGATATCAGCCTCTCCTTTGCTTGCTGAATTTATCGGTCCAGATGGCACTCATGCTAATGCTTTGTCAGCTATATCTGGCAAGTCAAGTGTTACAGAGCAACCTCTTGAACGCTTGATTAAAGCGGTCAAGTCAATATCACCAAAGGCATTAAGTGCATCTGTCAGTGACATAGGCTCAGTAGTGAGTATGATTGATAGGATAGCAGGTTCAGCACCAGGTAATGGATCTAGAGCTGCAGTTGGTGAGGATCTGGTTGCTATGACAAAATGTCGTCTACAGGCGAGAAACTTCATCACTCATGATGGAACAACAAATGGGACAAGGAAAATGAGGCGATACACTAGTGCCATGCCCTTAAATGTTGTGTCATCAGGTGGCAGCATGAATGATAGTTTCAAGCAGTTGACCTGTTCAGAGACATCTGACCTGGAGTCAACTGCAACGTCTCGTATCAAAAGGCCTAAGATTGAGGCTAATCATGCTCTTATGGAAGAAATAAGAGAAATAAACCGGCGGCTTATAGATACAGTTGTTGATATCAGTGATGAAGATGTTGATCCAAGTGCGGCTGCTGCTGAAGGGGGTGAAGGAACCATCGTCAAGTGCTCTTTTGATGCTGTGGCTCTCAGTCCCAACTTGAAATCGCAGTATGCTTCAGCACAAATGTCTCCGATTCAGCCATTAAGACTGCTTGTTCCGACAAATTATCCCAACTGCTCTCCAATACTGCTAGACAAGTTTCCGGTTGAAGTCAGTAAGGAGTATGAAGATCTTTCTGTGAAGGCAAAATCAAGGTTTAGCATCTCTTTACGAAGCATTTCACAGCCCATGTCCCTTGGGGAGATAGCAAGGGCCTGGGATAATTGTGCACGTACAGTTATTTCTGAGCATGCACAGCGAAGTGGTGGAGGCAGCTTCAGCTCAAAGTATGGGACATGGGAGAACTGCCTGAGTGCCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1349

Amino Acids

149.03

Weight (kDa)

9.39

Isoelectric Point (pI)

71.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 97 2.1e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1673
Acc36I ACCTGC 2 cut(s) 2302, 3281
AccB1I GGYRCC 1 cut(s) 3077
AccB7I CCANNNNNTGG 2 cut(s) 1243, 3308
AccI GTMKAC 1 cut(s) 3361
AccII CGCG 2 cut(s) 88, 2219
AcsI RAATTY 9 cut(s) 240, 577, 694, 749, 1096, 1984, 2197, 2434, 3113
AcuI CTGAAG 8 cut(s) 884, 932, 1892, 2411, 2825, 3684, 3726, 3988
AcyI GRCGYC 2 cut(s) 1460, 1670
AfaI GTAC 5 cut(s) 525, 1336, 1772, 1796, 3961
AhdI GACNNNNNGTC 2 cut(s) 3354, 3517
AhlI ACTAGT 1 cut(s) 3428
AjuI GAANNNNNNNTTGG 2 cut(s) 1940, 1972
AloI GAACNNNNNNTCC 2 cut(s) 3277, 3309
Alw21I GWGCWC 4 cut(s) 2353, 3055, 3695, 3958
Alw26I GTCTC 8 cut(s) 260, 279, 891, 1661, 1686, 3497, 3537, 3759
Alw44I GTGCAC 1 cut(s) 3954
AlwNI CAGNNNCTG 1 cut(s) 2644
AoxI GGCC 9 cut(s) 20, 329, 598, 1288, 2717, 2742, 2767, 3545, 3939
ApaI GGGCCC 1 cut(s) 1292
ApaLI GTGCAC 1 cut(s) 3954
ApoI RAATTY 9 cut(s) 240, 577, 694, 749, 1096, 1984, 2197, 2434, 3113
AseI ATTAAT 3 cut(s) 1241, 1992, 2418
Asp700I GAANNNNTTC 1 cut(s) 3904
AspLEI GCGC 1 cut(s) 1899
AspS9I GGNCC 9 cut(s) 21, 330, 1288, 1289, 2314, 2742, 3058, 3122, 3939
AsuC2I CCSGG 1 cut(s) 2721
AsuHPI GGTGA 8 cut(s) 502, 1929, 2675, 2919, 2961, 3216, 3341, 3683
AsuNHI GCTAGC 1 cut(s) 1928
AvaII GGWCC 3 cut(s) 2314, 3058, 3122
AxyI CCTNAGG 1 cut(s) 1455
BaeGI GKGCMC 2 cut(s) 1292, 3958
BaeI ACNNNNGTAYC 6 cut(s) 507, 540, 3415, 3448, 3603, 3636
BamHI GGATCC 3 cut(s) 1569, 1595, 2275
BanI GGYRCC 1 cut(s) 3077
BanII GRGCYC 5 cut(s) 510, 1292, 1660, 2327, 3055
BarI GAAGNNNNNNTAC 2 cut(s) 1754, 1786
BbsI GAAGAC 3 cut(s) 890, 990, 2199
Bbv12I GWGCWC 4 cut(s) 2353, 3055, 3695, 3958
BccI CCATC 8 cut(s) 1017, 1513, 2719, 2950, 2974, 3122, 3383, 3689
BcgI CGANNNNNNTGC 2 cut(s) 864, 898
BciVI GTATCC 1 cut(s) 109
BcnI CCSGG 1 cut(s) 2721
BcoDI GTCTC 8 cut(s) 260, 279, 891, 1661, 1686, 3497, 3537, 3759
BcuI ACTAGT 1 cut(s) 3428
BfaI CTAG 8 cut(s) 894, 1685, 1929, 1941, 2922, 3315, 3429, 3818
BfmI CTRYAG 6 cut(s) 873, 2306, 2405, 2763, 3321, 3362
BfoI RGCGCY 1 cut(s) 1900
BfuAI ACCTGC 2 cut(s) 2302, 3281
BfuI GTATCC 1 cut(s) 109
BglI GCCNNNNNGGC 1 cut(s) 2035
BglII AGATCT 1 cut(s) 3856
BlpI GCTNAGC 1 cut(s) 1248
Bme18I GGWCC 3 cut(s) 2314, 3058, 3122
BmeRI GACNNNNNGTC 2 cut(s) 3354, 3517
BmgT120I GGNCC 9 cut(s) 21, 330, 1288, 1289, 2314, 2742, 3058, 3122, 3939
BmtI GCTAGC 1 cut(s) 1932
BoxI GACNNNNGTC 2 cut(s) 1444, 2342
BpiI GAAGAC 3 cut(s) 890, 990, 2199
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpmI CTGGAG 2 cut(s) 1913, 3536
Bpu10I CCTNAGC 1 cut(s) 3054
Bpu1102I GCTNAGC 1 cut(s) 1248
BpuEI CTTGAG 5 cut(s) 12, 152, 1336, 1883, 2486
BpuMI CCSGG 1 cut(s) 2721
BsaAI YACGTR 1 cut(s) 3959
BsaBI GATNNNNATC 1 cut(s) 3642
BsaHI GRCGYC 2 cut(s) 1460, 1670
BsaI GGTCTC 1 cut(s) 260
BsaWI WCCGGW 2 cut(s) 143, 3829
BsaXI ACNNNNNCTCC 8 cut(s) 257, 287, 369, 399, 502, 532, 3042, 3072
Bse118I RCCGGY 1 cut(s) 3597
Bse1I ACTGG 7 cut(s) 220, 395, 478, 1312, 1348, 1708, 3061
Bse21I CCTNAGG 1 cut(s) 1455
Bse3DI GCAATG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 3642
BseGI GGATG 5 cut(s) 55, 768, 955, 2730, 2937
BseJI GATNNNNATC 1 cut(s) 3642
BseMI GCAATG 1 cut(s) 51
BseNI ACTGG 7 cut(s) 220, 395, 478, 1312, 1348, 1708, 3061
BseRI GAGGAG 3 cut(s) 79, 1748, 2844
BseSI GKGCMC 2 cut(s) 1292, 3958
BseYI CCCAGC 1 cut(s) 3014
BsgI GTGCAG 3 cut(s) 899, 1362, 1485
Bsh1236I CGCG 2 cut(s) 88, 2219
BshFI GGCC 9 cut(s) 22, 331, 600, 1290, 2719, 2744, 2769, 3547, 3941
BshNI GGYRCC 1 cut(s) 3077
BsiHKAI GWGCWC 4 cut(s) 2353, 3055, 3695, 3958
BsiSI CCGG 4 cut(s) 144, 2720, 3598, 3830
BsmAI GTCTC 8 cut(s) 260, 279, 891, 1661, 1686, 3497, 3537, 3759
BsmBI CGTCTC 4 cut(s) 891, 1661, 1686, 3537
BsmI GAATGC 2 cut(s) 2377, 2869
BsnI GGCC 9 cut(s) 22, 331, 600, 1290, 2719, 2744, 2769, 3547, 3941
Bso31I GGTCTC 1 cut(s) 260
Bsp120I GGGCCC 1 cut(s) 1288
Bsp1286I GDGCHC 8 cut(s) 510, 1292, 1660, 2327, 2353, 3055, 3695, 3958
Bsp1720I GCTNAGC 1 cut(s) 1248
BspANI GGCC 9 cut(s) 22, 331, 600, 1290, 2719, 2744, 2769, 3547, 3941
BspFNI CGCG 2 cut(s) 88, 2219
BspHI TCATGA 4 cut(s) 738, 1758, 2104, 3385
BspMAI CTGCAG 4 cut(s) 877, 2310, 2409, 3325
BspMI ACCTGC 2 cut(s) 2302, 3281
BspOI GCTAGC 1 cut(s) 1932
BspT107I GGYRCC 1 cut(s) 3077
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 51
BsrFI RCCGGY 1 cut(s) 3597
BsrI ACTGG 7 cut(s) 220, 395, 478, 1312, 1348, 1708, 3061
BssAI RCCGGY 1 cut(s) 3597
BssNI GRCGYC 2 cut(s) 1460, 1670
BssT1I CCWWGG 3 cut(s) 1330, 1395, 3922
Bst6I CTCTTC 2 cut(s) 119, 937
BstACI GRCGYC 2 cut(s) 1460, 1670
BstAPI GCANNNNNTGC 5 cut(s) 50, 2065, 2557, 2644, 3239
BstBAI YACGTR 1 cut(s) 3959
BstDSI CCRYGG 1 cut(s) 2217
BstENI CCTNNNNNAGG 2 cut(s) 339, 2976
BstF5I GGATG 5 cut(s) 55, 768, 955, 2730, 2937
BstFNI CGCG 2 cut(s) 88, 2219
BstH2I RGCGCY 1 cut(s) 1900
BstHHI GCGC 1 cut(s) 1899
BstMAI GTCTC 8 cut(s) 260, 279, 891, 1661, 1686, 3497, 3537, 3759
BstNSI RCATGY 6 cut(s) 1156, 1360, 1387, 2404, 2533, 3980
BstPAI GACNNNNGTC 2 cut(s) 1444, 2342
BstSFI CTRYAG 6 cut(s) 873, 2306, 2405, 2763, 3321, 3362
BstSLI GKGCMC 2 cut(s) 1292, 3958
BstUI CGCG 2 cut(s) 88, 2219
BstV2I GAAGAC 3 cut(s) 890, 990, 2199
BstX2I RGATCY 8 cut(s) 344, 1569, 1595, 2275, 2804, 3311, 3334, 3856
BstYI RGATCY 8 cut(s) 344, 1569, 1595, 2275, 2804, 3311, 3334, 3856
Bsu36I CCTNAGG 1 cut(s) 1455
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 9 cut(s) 22, 331, 600, 1290, 2719, 2744, 2769, 3547, 3941
BtgI CCRYGG 1 cut(s) 2217
BtsCI GGATG 5 cut(s) 55, 768, 955, 2730, 2937
BtsI GCAGTG 2 cut(s) 882, 2303
BtsIMutI CAGTG 8 cut(s) 402, 882, 2294, 2303, 2353, 2698, 3256, 3634
BveI ACCTGC 2 cut(s) 2302, 3281
CaiI CAGNNNCTG 1 cut(s) 2644
CciI TCATGA 4 cut(s) 738, 1758, 2104, 3385
CfoI GCGC 1 cut(s) 1899
Cfr10I RCCGGY 1 cut(s) 3597
Cfr13I GGNCC 9 cut(s) 21, 330, 1288, 1289, 2314, 2742, 3058, 3122, 3939
Cfr42I CCGCGG 1 cut(s) 2220
CseI GACGC 1 cut(s) 1449
CsiI ACCWGGT 1 cut(s) 3301
Csp6I GTAC 5 cut(s) 524, 1335, 1771, 1795, 3960
CspCI CAANNNNNGTGG 2 cut(s) 819, 854
CviQI GTAC 5 cut(s) 524, 1335, 1771, 1795, 3960
DriI GACNNNNNGTC 2 cut(s) 3354, 3517
Eam1104I CTCTTC 2 cut(s) 119, 937
Eam1105I GACNNNNNGTC 2 cut(s) 3354, 3517
EarI CTCTTC 2 cut(s) 119, 937
EciI GGCGGA 1 cut(s) 450
Ecl136II GAGCTC 1 cut(s) 3053
Eco130I CCWWGG 3 cut(s) 1330, 1395, 3922
Eco147I AGGCCT 1 cut(s) 3547
Eco24I GRGCYC 5 cut(s) 510, 1292, 1660, 2327, 3055
Eco31I GGTCTC 1 cut(s) 260
Eco32I GATATC 2 cut(s) 3090, 3625
Eco47I GGWCC 3 cut(s) 2314, 3058, 3122
Eco53kI GAGCTC 1 cut(s) 3053
Eco57I CTGAAG 8 cut(s) 884, 932, 1892, 2411, 2825, 3684, 3726, 3988
Eco81I CCTNAGG 1 cut(s) 1455
EcoICRI GAGCTC 1 cut(s) 3053
EcoNI CCTNNNNNAGG 2 cut(s) 339, 2976
EcoO109I RGGNCCY 6 cut(s) 21, 330, 1289, 2314, 2742, 3939
EcoRI GAATTC 2 cut(s) 577, 694
EcoRV GATATC 2 cut(s) 3090, 3625
EcoT14I CCWWGG 3 cut(s) 1330, 1395, 3922
EcoT22I ATGCAT 1 cut(s) 1275
EcoT38I GRGCYC 5 cut(s) 510, 1292, 1660, 2327, 3055
ErhI CCWWGG 3 cut(s) 1330, 1395, 3922
Esp3I CGTCTC 4 cut(s) 891, 1661, 1686, 3537
FalI AAGNNNNNCTT 2 cut(s) 930, 962
FauI CCCGC 1 cut(s) 1551
FauNDI CATATG 2 cut(s) 789, 2055
FblI GTMKAC 1 cut(s) 3361
FokI GGATG 5 cut(s) 62, 755, 942, 2737, 2924
FriOI GRGCYC 5 cut(s) 510, 1292, 1660, 2327, 3055
FspBI CTAG 8 cut(s) 894, 1685, 1929, 1941, 2922, 3315, 3429, 3818
GlaI GCGC 1 cut(s) 1898
GsaI CCCAGC 1 cut(s) 3018
GsuI CTGGAG 2 cut(s) 1913, 3536
HaeII RGCGCY 1 cut(s) 1900
HaeIII GGCC 9 cut(s) 22, 331, 600, 1290, 2719, 2744, 2769, 3547, 3941
HapII CCGG 4 cut(s) 144, 2720, 3598, 3830
HgaI GACGC 1 cut(s) 1449
HhaI GCGC 1 cut(s) 1899
Hin1I GRCGYC 2 cut(s) 1460, 1670
Hin6I GCGC 1 cut(s) 1897
HinP1I GCGC 1 cut(s) 1897
HincII GTYRAC 4 cut(s) 2701, 2887, 3492, 3522
HindII GTYRAC 4 cut(s) 2701, 2887, 3492, 3522
HpaI GTTAAC 1 cut(s) 2701
HpaII CCGG 4 cut(s) 144, 2720, 3598, 3830
HphI GGTGA 8 cut(s) 502, 1929, 2675, 2919, 2961, 3216, 3341, 3683
Hpy166II GTNNAC 7 cut(s) 2701, 2887, 2927, 3362, 3492, 3522, 3956
Hpy8I GTNNAC 7 cut(s) 2701, 2887, 2927, 3362, 3492, 3522, 3956
HpyCH4IV ACGT 4 cut(s) 1670, 1679, 3530, 3958
HpySE526I ACGT 4 cut(s) 1670, 1679, 3530, 3958
Hsp92I GRCGYC 2 cut(s) 1460, 1670
HspAI GCGC 1 cut(s) 1897
KspAI GTTAAC 1 cut(s) 2701
KspI CCGCGG 1 cut(s) 2220
LmnI GCTCC 6 cut(s) 66, 1894, 2046, 2356, 2968, 3050
MabI ACCWGGT 1 cut(s) 3301
MaeI CTAG 8 cut(s) 894, 1685, 1929, 1941, 2922, 3315, 3429, 3818
MaeII ACGT 4 cut(s) 1670, 1679, 3530, 3958
MfeI CAATTG 3 cut(s) 285, 2126, 2483
MflI RGATCY 8 cut(s) 344, 1569, 1595, 2275, 2804, 3311, 3334, 3856
MhlI GDGCHC 8 cut(s) 510, 1292, 1660, 2327, 2353, 3055, 3695, 3958
MlyI GAGTC 5 cut(s) 511, 1426, 1823, 2332, 3527
MmeI TCCRAC 7 cut(s) 204, 517, 796, 2356, 3004, 3028, 3809
Mph1103I ATGCAT 1 cut(s) 1275
MroXI GAANNNNTTC 1 cut(s) 3904
MslI CAYNNNNRTG 2 cut(s) 1713, 3141
MspA1I CMGCKG 2 cut(s) 2219, 2644
MspI CCGG 4 cut(s) 144, 2720, 3598, 3830
MunI CAATTG 3 cut(s) 285, 2126, 2483
Mva1269I GAATGC 2 cut(s) 2377, 2869
MvnI CGCG 2 cut(s) 88, 2219
NciI CCSGG 1 cut(s) 2721
NdeI CATATG 2 cut(s) 789, 2055
NheI GCTAGC 1 cut(s) 1928
NmuCI GTSAC 5 cut(s) 381, 1461, 2073, 2862, 3251
NsiI ATGCAT 1 cut(s) 1275
NspI RCATGY 6 cut(s) 1156, 1360, 1387, 2404, 2533, 3980
PaeI GCATGC 2 cut(s) 1360, 3980
PagI TCATGA 4 cut(s) 738, 1758, 2104, 3385
PasI CCCWGGG 1 cut(s) 3083
PceI AGGCCT 1 cut(s) 3547
PcsI WCGNNNNNNNCGW 1 cut(s) 1676
PctI GAATGC 2 cut(s) 2377, 2869
PdmI GAANNNNTTC 1 cut(s) 3904
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 2 cut(s) 1243, 3308
PfoI TCCNGGA 2 cut(s) 340, 2278
PleI GAGTC 5 cut(s) 511, 1425, 1823, 2332, 3526
PpsI GAGTC 5 cut(s) 511, 1425, 1823, 2332, 3526
Ppu21I YACGTR 1 cut(s) 3959
PpuMI RGGWCCY 1 cut(s) 2314
PshAI GACNNNNGTC 2 cut(s) 1444, 2342
PshBI ATTAAT 3 cut(s) 1241, 1992, 2418
Psp124BI GAGCTC 1 cut(s) 3055
Psp5II RGGWCCY 1 cut(s) 2314
PspFI CCCAGC 1 cut(s) 3014
PspOMI GGGCCC 1 cut(s) 1288
PspPI GGNCC 9 cut(s) 21, 330, 1288, 1289, 2314, 2742, 3058, 3122, 3939
PspPPI RGGWCCY 1 cut(s) 2314
PstI CTGCAG 4 cut(s) 877, 2310, 2409, 3325
PstNI CAGNNNCTG 1 cut(s) 2644
PsuI RGATCY 8 cut(s) 344, 1569, 1595, 2275, 2804, 3311, 3334, 3856
PsyI GACNNNGTC 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 2644
RsaI GTAC 5 cut(s) 525, 1336, 1772, 1796, 3961
RsaNI GTAC 5 cut(s) 524, 1335, 1771, 1795, 3960
RseI CAYNNNNRTG 2 cut(s) 1713, 3141
SacI GAGCTC 1 cut(s) 3055
SacII CCGCGG 1 cut(s) 2220
Sau96I GGNCC 9 cut(s) 21, 330, 1288, 1289, 2314, 2742, 3058, 3122, 3939
SchI GAGTC 5 cut(s) 511, 1426, 1823, 2332, 3527
SduI GDGCHC 8 cut(s) 510, 1292, 1660, 2327, 2353, 3055, 3695, 3958
SexAI ACCWGGT 1 cut(s) 3301
SfcI CTRYAG 6 cut(s) 873, 2306, 2405, 2763, 3321, 3362
Sfr303I CCGCGG 1 cut(s) 2220
SgrBI CCGCGG 1 cut(s) 2220
SinI GGWCC 3 cut(s) 2314, 3058, 3122
SmiMI CAYNNNNRTG 2 cut(s) 1713, 3141
SmlI CTYRAG 5 cut(s) 27, 167, 1351, 1862, 2501
SmoI CTYRAG 5 cut(s) 27, 167, 1351, 1862, 2501
SpeI ACTAGT 1 cut(s) 3428
SphI GCATGC 2 cut(s) 1360, 3980
SseBI AGGCCT 1 cut(s) 3547
SspI AATATT 1 cut(s) 1044
SspMI CTAG 8 cut(s) 894, 1685, 1929, 1941, 2922, 3315, 3429, 3818
SstI GAGCTC 1 cut(s) 3055
StuI AGGCCT 1 cut(s) 3547
StyI CCWWGG 3 cut(s) 1330, 1395, 3922
TaiI ACGT 4 cut(s) 1673, 1682, 3533, 3961
TaqII GACCGA 1 cut(s) 3110
TatI WGTACW 1 cut(s) 1770
TauI GCSGC 7 cut(s) 91, 976, 2032, 2170, 3604, 3656, 4046
TscAI CASTG 8 cut(s) 402, 882, 2301, 2310, 2353, 2698, 3256, 3634
TseFI GTSAC 5 cut(s) 381, 1461, 2073, 2862, 3251
Tsp45I GTSAC 5 cut(s) 381, 1461, 2073, 2862, 3251
TspGWI ACGGA 2 cut(s) 250, 2241
TspRI CASTG 8 cut(s) 402, 882, 2301, 2310, 2353, 2698, 3256, 3634
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 2 cut(s) 1243, 3308
VneI GTGCAC 1 cut(s) 3954
VpaK11BI GGWCC 3 cut(s) 2314, 3058, 3122
VspI ATTAAT 3 cut(s) 1241, 1992, 2418
XagI CCTNNNNNAGG 2 cut(s) 339, 2976
XapI RAATTY 9 cut(s) 240, 577, 694, 749, 1096, 1984, 2197, 2434, 3113
XbaI TCTAGA 2 cut(s) 1684, 3314
XceI RCATGY 6 cut(s) 1156, 1360, 1387, 2404, 2533, 3980
XmiI GTMKAC 1 cut(s) 3361
XmnI GAANNNNTTC 1 cut(s) 3904
XspI CTAG 8 cut(s) 894, 1685, 1929, 1941, 2922, 3315, 3429, 3818
ZraI GACGTC 1 cut(s) 1671
Zsp2I ATGCAT 1 cut(s) 1275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.